Rorug02G0625600

Pectate lyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0010647
Physical Location & Seq
Reverse (-)
74172986 .. 74176231
3246 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0625600.1

Sequence Viewer

Length: 2322 bp
ATGGCCCCAAATTTGAGCAGTGGCTCTGCCGTCGTTAACGTCGACAATGGCGCAAACCCAAGTCCTTTTACCTTTCAAGGTTCCAACTTGACGGCCAACGGCCACGTCATCCTCTCCGACGTCCCCCAAAACATAACACTCCGCCAATCACCCTACACCATCAACAAGTCCTCCGCCGCCCCCGGCTCTTTTGTTGGCTTCGACGCCGCCGAGGCCAGCAGCCGCCACGTGGTGCCCGTCGGCCAGCTGAAGGACATCCGCTTCATGAGCATCTTCCGGTTCAAGGTCTGGTGGACCACTCACTGGGTCGGATCCAACGGCCGCGACCTCGAGCAGGAGACCCAGATCGTCATCCTCGAAAACTCCGACGCCGGCAGGCCCTACGTCGTCATCCTCCCGCTCCTCGAAGGCGGCTTCCGCGCTTCCATCCAGCCAGGCTCCGAGGACTACCTCGACGTCTGTGTAGAGAGCGGTTCAACGCAGGTCAACGAAAAGGGATTCTCCGGCGTGCTTTATCTTCACGCCGGAGAAGATCCCTTCACGCTGGTGAAGGACGCCGTCAAGGTGGCCAAGGCGCACTTGGGAACGTTCAATCTGTTGGAGGAGAAGACGCCACCTGGCATCGTCGACAAGTTCGGTTGGTGCACGTGGGATGCTTTTTACCTCACTGTGCACCCTCGCGGCGTCATTGATGGCGTGAGGAAGCTTGTGGAGGGTGGGTGCCCTCCAGGGCTTGTGTTGCTGGACGACGGGTGGCAGTCCATCGGCCACGACTCTGATCCCATCACCAAAGAAGGGATGGGCCAGACCGCCGCCGGTGAGCAAATGCCGTGCAGGCTGCTCAAGTTCAAAGAGAACTACAAGTTCATAGACTATGTCAGCTCCAAGGACCCTAGTAGCCGAGGCATGGGTGCGTTTGTGAAGGACTTGAAGGACGAGTTCAAGAGCGTTGACTACGTGTACGTGTGGCACGCGCTGTGTGGGTACTGGGGTGGGATCCGTCCCAATGTTCCTGGCCTGCCAGAGGCGGTGGTTGTTATGCCGGAACTGTCACCGGGGTTGAAGAACACGATGGAAGATCTGGCCGTGGATAAGATAGTCAACACTGGGATTGGGCTGGTCCCACCGGAAATGGTTAACCAGATGTATGAAGGGCTCCACTCTCACCTGAAGTCCGTCGGGATCGACGGCGTCAAGGTCGACGTTATCCATTTGTTGGAAATGTTGTGTGAGAACTATGGTGGCCGTGTGAACCTGGCTAAAGCATATTACGATGCCCTAACTGCTTCAGTGAGGAAGCACTTCGACGGCAATGGTGTCATTGCTAGCATGGAGCACTGTAACGACTTCATGTTCCTTGGAACCGAAGCCATCACTCTCGGCCGTGTCGGTGACGATTTCTGGTGCACTGACCCCTCTGGTGATCCCAACGGTACCTTTTGGTTGCAAGGGTGTCACATGGTTCATTGTGCTTACAATAGCTTATGGATGGGTAACTTCATACAGCCGGATTGGGACATGTTCCAGTCCACTCATCCCTGTGCCGCTTTCCATGCTGCCTCTAGGGCAATCTCCGGCGGTCCTATTTATGTAAGTGACACTGTGGGGAAGCACAACTTTGACTTGCTCAAAACCCTAGTGGTGCCTGACGGCTCTATCCTTCGCTGCGAGTACTATGCTCTCCCTACCCGCGATTGTCTTTTTGAGGATCCTCTCCATGATGGCCGCACCATGCTCAAAATTTGGAACCTCAACAAGTTCAATGGAGTTCTTGGAGCCTTTAACTGCCAAGGAGGAGGCTGGAGCCGTGAGACTAGGCGAAACCAATGTTTTTCTCAATTCGTGGGAAAGGTGACCTCCAAGGCCAACCCTAAAGACATTGAGTGGAACAGTGGCAAGAGTCCAATTTCCATTGAAGGAGTGCAAGTGTTTGCCTTGTACTATCACCAGGCCAAGAAGCTAGTCCTGTCTAAGCCAACCGAGGATGTGGAGTTGTCACTGGAGCCCTTCAACTTTGAGCTAATTGTCATTTCTCCGGTGACTACTTTGCCTAGCAAGTCTGTCCAATTTGCCCCTATTGGCTTAGTGAACATGCTCAACACCGGCGGTGCTGTCAAGTCGTTGGTTTACAGTGAGGAGGGAAACTCTGTTCAAATTGGGGTGAAGGGCACCGGAGAGATGAGGGTGTTTGCGTCTGAGAAGCCACTGGGTTGCAAGATTGATGGAAGCTGTGTCGCTTTTGAGTATGAAGAGAACATGGTTAGAGTTCATGTGCCATGGACTGGTACATCCACCTTGTCTATTGTGGAGTACATATTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00040 ko02024 map00040 map02024
Pfam Domains
Protein Families
Pec_lyase_C

Protein Analysis

773

Amino Acids

84.8

Weight (kDa)

5.44

Isoelectric Point (pI)

31.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Raffinose_syn PF05691 27 - 756 0.0 Raffinose synthase or seed imbibition protein Sip1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 123, 459
Acc36I ACCTGC 1 cut(s) 472
Acc65I GGTACC 1 cut(s) 1433
AccB1I GGYRCC 5 cut(s) 232, 720, 1433, 1642, 2168
AccB7I CCANNNNNTGG 3 cut(s) 303, 1216, 2282
AccBSI CCGCTC 2 cut(s) 400, 471
AccI GTMKAC 3 cut(s) 42, 627, 1200
AccII CGCG 5 cut(s) 324, 420, 681, 974, 1692
AclI AACGTT 1 cut(s) 587
AcsI RAATTY 2 cut(s) 10, 1740
AcuI CTGAAG 3 cut(s) 269, 1190, 1272
AcvI CACGTG 2 cut(s) 229, 648
AcyI GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
AdeI CACNNNGTG 1 cut(s) 232
AfaI GTAC 7 cut(s) 962, 986, 1435, 1673, 1940, 2287, 2312
AflIII ACRYGT 3 cut(s) 957, 963, 1518
AjiI CACGTC 1 cut(s) 106
AjnI CCWGG 6 cut(s) 433, 616, 727, 1012, 1254, 1947
AleI CACNNNNGTG 1 cut(s) 545
AloI GAACNNNNNNTCC 2 cut(s) 2133, 2165
AluBI AGCT 7 cut(s) 247, 706, 882, 1482, 1960, 2020, 2229
AluI AGCT 7 cut(s) 247, 706, 882, 1482, 1960, 2020, 2229
Alw21I GWGCWC 4 cut(s) 647, 675, 1338, 1409
Alw26I GTCTC 2 cut(s) 332, 1805
Alw44I GTGCAC 3 cut(s) 643, 671, 1405
Ama87I CYCGRG 1 cut(s) 329
ApaLI GTGCAC 3 cut(s) 643, 671, 1405
ApeKI GCWGC 4 cut(s) 219, 838, 1556, 1665
ApoI RAATTY 2 cut(s) 10, 1740
Asp700I GAANNNNTTC 1 cut(s) 1301
Asp718I GGTACC 1 cut(s) 1433
AspLEI GCGC 4 cut(s) 53, 422, 577, 976
AspS9I GGNCC 7 cut(s) 4, 294, 378, 802, 889, 1120, 1580
AsuC2I CCSGG 2 cut(s) 183, 1056
AsuNHI GCTAGC 1 cut(s) 1325
AvaI CYCGRG 1 cut(s) 329
AvaII GGWCC 4 cut(s) 294, 889, 1120, 1580
BaeGI GKGCMC 6 cut(s) 237, 647, 675, 725, 1409, 2171
BaeI ACNNNNGTAYC 2 cut(s) 976, 1009
BalI TGGCCA 1 cut(s) 569
BamHI GGATCC 3 cut(s) 311, 996, 1708
BanI GGYRCC 5 cut(s) 232, 720, 1433, 1642, 2168
BanII GRGCYC 2 cut(s) 1158, 2007
BbrPI CACGTG 2 cut(s) 229, 648
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 4 cut(s) 647, 675, 1338, 1409
BbvI GCAGC 4 cut(s) 231, 825, 1543, 1652
BcgI CGANNNNNNTGC 2 cut(s) 1658, 1692
BciT130I CCWGG 6 cut(s) 435, 618, 729, 1014, 1256, 1949
BcnI CCSGG 2 cut(s) 183, 1056
BcoDI GTCTC 2 cut(s) 332, 1805
BfaI CTAG 8 cut(s) 894, 1326, 1563, 1637, 1815, 1961, 2052, 2320
BfuAI ACCTGC 1 cut(s) 472
BglI GCCNNNNNGGC 3 cut(s) 212, 835, 1565
BglII AGATCT 1 cut(s) 1078
BmcAI AGTACT 1 cut(s) 1673
Bme1390I CCNGG 8 cut(s) 183, 435, 618, 729, 1014, 1056, 1256, 1949
Bme18I GGWCC 4 cut(s) 294, 889, 1120, 1580
BmeT110I CYCGRG 1 cut(s) 329
BmgBI CACGTC 1 cut(s) 106
BmgT120I GGNCC 7 cut(s) 4, 294, 378, 802, 889, 1120, 1580
BmrFI CCNGG 8 cut(s) 183, 435, 618, 729, 1014, 1056, 1256, 1949
BmrI ACTGGG 4 cut(s) 313, 997, 1116, 2216
BmsI GCATC 4 cut(s) 279, 630, 643, 1264
BmtI GCTAGC 1 cut(s) 1329
BmuI ACTGGG 4 cut(s) 313, 997, 1116, 2216
BpiI GAAGAC 1 cut(s) 614
BplI GAGNNNNNCTC 2 cut(s) 2129, 2161
BpmI CTGGAG 3 cut(s) 711, 1822, 2021
BpuEI CTTGAG 1 cut(s) 827
BpuMI CCSGG 2 cut(s) 183, 1056
BsaAI YACGTR 4 cut(s) 229, 648, 958, 964
BsaBI GATNNNNATC 1 cut(s) 350
BsaHI GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
BsaI GGTCTC 1 cut(s) 332
BsaWI WCCGGW 4 cut(s) 276, 1126, 2035, 2171
BsaXI ACNNNNNCTCC 6 cut(s) 155, 185, 704, 734, 1911, 1941
Bse118I RCCGGY 3 cut(s) 371, 815, 2102
Bse1I ACTGG 7 cut(s) 308, 992, 1111, 1525, 2004, 2211, 2287
Bse3DI GCAATG 2 cut(s) 1318, 1320
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 6 cut(s) 435, 618, 729, 1014, 1256, 1949
BseJI GATNNNNATC 1 cut(s) 350
BseMI GCAATG 2 cut(s) 1318, 1320
BseMII CTCAG 1 cut(s) 2187
BseNI ACTGG 7 cut(s) 308, 992, 1111, 1525, 2004, 2211, 2287
BseRI GAGGAG 4 cut(s) 392, 617, 1809, 2150
BseSI GKGCMC 6 cut(s) 237, 647, 675, 725, 1409, 2171
BseX3I CGGCCG 2 cut(s) 319, 1381
BseXI GCAGC 4 cut(s) 231, 825, 1543, 1652
BsgI GTGCAG 1 cut(s) 853
Bsh1236I CGCG 5 cut(s) 324, 420, 681, 974, 1692
Bsh1285I CGRYCG 2 cut(s) 322, 1384
BshNI GGYRCC 5 cut(s) 232, 720, 1433, 1642, 2168
BsiEI CGRYCG 2 cut(s) 322, 1384
BsiHKAI GWGCWC 4 cut(s) 647, 675, 1338, 1409
BsiHKCI CYCGRG 1 cut(s) 329
BslFI GGGAC 4 cut(s) 107, 987, 1106, 1529
BsmAI GTCTC 2 cut(s) 332, 1805
BsmFI GGGAC 4 cut(s) 107, 987, 1106, 1529
Bso31I GGTCTC 1 cut(s) 332
BsoBI CYCGRG 1 cut(s) 329
Bsp1286I GDGCHC 9 cut(s) 237, 647, 675, 725, 1158, 1338, 1409, 2007, 2171
Bsp143I GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
Bsp19I CCATGG 1 cut(s) 2276
BspCNI CTCAG 1 cut(s) 2188
BspFNI CGCG 5 cut(s) 324, 420, 681, 974, 1692
BspHI TCATGA 1 cut(s) 264
BspMI ACCTGC 1 cut(s) 472
BspOI GCTAGC 1 cut(s) 1329
BspT107I GGYRCC 5 cut(s) 232, 720, 1433, 1642, 2168
BspTNI GGTCTC 1 cut(s) 332
BsrBI CCGCTC 2 cut(s) 400, 471
BsrDI GCAATG 2 cut(s) 1318, 1320
BsrFI RCCGGY 3 cut(s) 371, 815, 2102
BsrI ACTGG 7 cut(s) 308, 992, 1111, 1525, 2004, 2211, 2287
BssAI RCCGGY 3 cut(s) 371, 815, 2102
BssMI GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
BssNI GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
BssT1I CCWWGG 6 cut(s) 570, 885, 1357, 1789, 1860, 2276
Bst2UI CCWGG 6 cut(s) 435, 618, 729, 1014, 1256, 1949
Bst4CI ACNGT 7 cut(s) 670, 1050, 1340, 1433, 1603, 1892, 2132
Bst6I CTCTTC 1 cut(s) 2244
BstACI GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
BstBAI YACGTR 4 cut(s) 229, 648, 958, 964
BstC8I GCNNGC 9 cut(s) 217, 245, 373, 377, 509, 836, 972, 1019, 1327
BstDEI CTNAG 3 cut(s) 1971, 2083, 2196
BstDSI CCRYGG 2 cut(s) 1086, 2276
BstEII GGTNACC 1 cut(s) 1852
BstENI CCTNNNNNAGG 2 cut(s) 332, 1022
BstFNI CGCG 5 cut(s) 324, 420, 681, 974, 1692
BstHHI GCGC 4 cut(s) 53, 422, 577, 976
BstKTI GATC 9 cut(s) 314, 348, 535, 781, 999, 1081, 1185, 1426, 1711
BstMAI GTCTC 2 cut(s) 332, 1805
BstMBI GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
BstMCI CGRYCG 2 cut(s) 322, 1384
BstMWI GCNNNNNNNGC 8 cut(s) 212, 267, 417, 739, 835, 1283, 1553, 1565
BstNI CCWGG 6 cut(s) 435, 618, 729, 1014, 1256, 1949
BstNSI RCATGY 2 cut(s) 1522, 2095
BstPI GGTNACC 1 cut(s) 1852
BstSCI CCNGG 8 cut(s) 181, 433, 616, 727, 1012, 1054, 1254, 1947
BstSLI GKGCMC 6 cut(s) 237, 647, 675, 725, 1409, 2171
BstUI CGCG 5 cut(s) 324, 420, 681, 974, 1692
BstV1I GCAGC 4 cut(s) 231, 825, 1543, 1652
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 5 cut(s) 311, 532, 996, 1078, 1708
BstYI RGATCY 5 cut(s) 311, 532, 996, 1078, 1708
BstZI CGGCCG 2 cut(s) 319, 1381
BtgI CCRYGG 2 cut(s) 1086, 2276
BtrI CACGTC 1 cut(s) 106
BtsI GCAGTG 1 cut(s) 25
BveI ACCTGC 1 cut(s) 472
Cac8I GCNNGC 9 cut(s) 217, 245, 373, 377, 509, 836, 972, 1019, 1327
CciI TCATGA 1 cut(s) 264
CfoI GCGC 4 cut(s) 53, 422, 577, 976
Cfr10I RCCGGY 3 cut(s) 371, 815, 2102
Cfr13I GGNCC 7 cut(s) 4, 294, 378, 802, 889, 1120, 1580
CseI GACGC 7 cut(s) 212, 377, 563, 619, 673, 1180, 2181
Csp6I GTAC 7 cut(s) 961, 985, 1434, 1672, 1939, 2286, 2311
CviQI GTAC 7 cut(s) 961, 985, 1434, 1672, 1939, 2286, 2311
DdeI CTNAG 3 cut(s) 1971, 2083, 2196
DpnI GATC 9 cut(s) 313, 347, 534, 780, 998, 1080, 1184, 1425, 1710
DpnII GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
DraIII CACNNNGTG 1 cut(s) 232
EagI CGGCCG 2 cut(s) 319, 1381
Eam1104I CTCTTC 1 cut(s) 2244
EarI CTCTTC 1 cut(s) 2244
EciI GGCGGA 2 cut(s) 131, 163
EclXI CGGCCG 2 cut(s) 319, 1381
Eco130I CCWWGG 6 cut(s) 570, 885, 1357, 1789, 1860, 2276
Eco24I GRGCYC 2 cut(s) 1158, 2007
Eco31I GGTCTC 1 cut(s) 332
Eco47I GGWCC 4 cut(s) 294, 889, 1120, 1580
Eco52I CGGCCG 2 cut(s) 319, 1381
Eco57I CTGAAG 3 cut(s) 269, 1190, 1272
Eco72I CACGTG 2 cut(s) 229, 648
Eco88I CYCGRG 1 cut(s) 329
Eco91I GGTNACC 1 cut(s) 1852
EcoNI CCTNNNNNAGG 2 cut(s) 332, 1022
EcoO109I RGGNCCY 2 cut(s) 378, 889
EcoO65I GGTNACC 1 cut(s) 1852
EcoRII CCWGG 6 cut(s) 433, 616, 727, 1012, 1254, 1947
EcoT14I CCWWGG 6 cut(s) 570, 885, 1357, 1789, 1860, 2276
EcoT38I GRGCYC 2 cut(s) 1158, 2007
ErhI CCWWGG 6 cut(s) 570, 885, 1357, 1789, 1860, 2276
FalI AAGNNNNNCTT 4 cut(s) 563, 595, 1601, 1633
FaqI GGGAC 4 cut(s) 107, 987, 1106, 1529
FauI CCCGC 2 cut(s) 405, 1697
FblI GTMKAC 3 cut(s) 42, 627, 1200
FriOI GRGCYC 2 cut(s) 1158, 2007
FspBI CTAG 8 cut(s) 894, 1326, 1563, 1637, 1815, 1961, 2052, 2320
GlaI GCGC 4 cut(s) 52, 421, 576, 975
GsuI CTGGAG 3 cut(s) 711, 1822, 2021
HgaI GACGC 7 cut(s) 212, 377, 563, 619, 673, 1180, 2181
HhaI GCGC 4 cut(s) 53, 422, 577, 976
Hin1I GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
Hin6I GCGC 4 cut(s) 51, 420, 575, 974
HinP1I GCGC 4 cut(s) 51, 420, 575, 974
HincII GTYRAC 8 cut(s) 37, 43, 487, 628, 952, 1102, 1138, 1201
HindII GTYRAC 8 cut(s) 37, 43, 487, 628, 952, 1102, 1138, 1201
HindIII AAGCTT 1 cut(s) 704
HinfI GANTC 3 cut(s) 498, 773, 1900
HpaI GTTAAC 2 cut(s) 37, 1138
Hpy188I TCNGA 6 cut(s) 118, 311, 367, 442, 778, 2197
Hpy188III TCNNGA 3 cut(s) 265, 943, 1180
HpyCH4III ACNGT 7 cut(s) 670, 1050, 1340, 1433, 1603, 1892, 2132
HpyCH4V TGCA 7 cut(s) 645, 673, 834, 1407, 1447, 1924, 2214
HpyF10VI GCNNNNNNNGC 8 cut(s) 212, 267, 417, 739, 835, 1283, 1553, 1565
HpyF3I CTNAG 3 cut(s) 1971, 2083, 2196
Hsp92I GRCGYC 8 cut(s) 120, 204, 369, 456, 555, 611, 684, 1191
HspAI GCGC 4 cut(s) 51, 420, 575, 974
KpnI GGTACC 1 cut(s) 1437
KroI GCCGGC 1 cut(s) 371
KroNI GCCGGC 1 cut(s) 373
KspAI GTTAAC 2 cut(s) 37, 1138
Kzo9I GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
LmnI GCTCC 8 cut(s) 405, 443, 887, 1161, 1333, 1775, 1803, 2002
Lsp1109I GCAGC 4 cut(s) 231, 825, 1543, 1652
LweI GCATC 4 cut(s) 279, 630, 643, 1264
MaeI CTAG 8 cut(s) 894, 1326, 1563, 1637, 1815, 1961, 2052, 2320
MaeIII GTNAC 9 cut(s) 1050, 1340, 1391, 1454, 1493, 1595, 1852, 1995, 2038
MalI GATC 9 cut(s) 313, 347, 534, 780, 998, 1080, 1184, 1425, 1710
MbiI CCGCTC 2 cut(s) 400, 471
MboI GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
MboII GAAGA 7 cut(s) 265, 509, 542, 619, 1075, 1088, 2261
MflI RGATCY 5 cut(s) 311, 532, 996, 1078, 1708
MhlI GDGCHC 9 cut(s) 237, 647, 675, 725, 1158, 1338, 1409, 2007, 2171
MlsI TGGCCA 1 cut(s) 569
MluCI AATT 7 cut(s) 10, 1740, 1838, 1905, 2022, 2066, 2154
MluNI TGGCCA 1 cut(s) 569
MlyI GAGTC 2 cut(s) 767, 1909
MmeI TCCRAC 7 cut(s) 108, 141, 289, 339, 390, 579, 1197
Mox20I TGGCCA 1 cut(s) 569
MroNI GCCGGC 1 cut(s) 371
MroXI GAANNNNTTC 1 cut(s) 1301
MscI TGGCCA 1 cut(s) 569
MseI TTAA 3 cut(s) 36, 1137, 1782
MslI CAYNNNNRTG 2 cut(s) 545, 1539
Msp20I TGGCCA 1 cut(s) 569
MspA1I CMGCKG 1 cut(s) 247
MspR9I CCNGG 8 cut(s) 183, 435, 618, 729, 1014, 1056, 1256, 1949
MvaI CCWGG 6 cut(s) 435, 618, 729, 1014, 1256, 1949
MvnI CGCG 5 cut(s) 324, 420, 681, 974, 1692
MwoI GCNNNNNNNGC 8 cut(s) 212, 267, 417, 739, 835, 1283, 1553, 1565
NaeI GCCGGC 1 cut(s) 373
NciI CCSGG 2 cut(s) 183, 1056
NcoI CCATGG 1 cut(s) 2276
NdeII GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
NgoMIV GCCGGC 1 cut(s) 371
NheI GCTAGC 1 cut(s) 1325
NmeAIII GCCGAG 3 cut(s) 235, 926, 1359
NmuCI GTSAC 7 cut(s) 1050, 1391, 1454, 1595, 1852, 1995, 2038
NspI RCATGY 2 cut(s) 1522, 2095
OliI CACNNNNGTG 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 329
PagI TCATGA 1 cut(s) 264
PciI ACATGT 1 cut(s) 1518
PcsI WCGNNNNNNNCGW 5 cut(s) 39, 207, 234, 354, 363
PdiI GCCGGC 1 cut(s) 373
PdmI GAANNNNTTC 1 cut(s) 1301
PfeI GAWTC 1 cut(s) 498
PflFI GACNNNGTC 3 cut(s) 557, 875, 1190
PflMI CCANNNNNTGG 3 cut(s) 303, 1216, 2282
PleI GAGTC 2 cut(s) 767, 1908
PmaCI CACGTG 2 cut(s) 229, 648
PmlI CACGTG 2 cut(s) 229, 648
PpsI GAGTC 2 cut(s) 767, 1908
Ppu21I YACGTR 4 cut(s) 229, 648, 958, 964
PpuMI RGGWCCY 1 cut(s) 889
PscI ACATGT 1 cut(s) 1518
Psp1406I AACGTT 1 cut(s) 587
Psp5II RGGWCCY 1 cut(s) 889
Psp6I CCWGG 6 cut(s) 433, 616, 727, 1012, 1254, 1947
PspCI CACGTG 2 cut(s) 229, 648
PspEI GGTNACC 1 cut(s) 1852
PspGI CCWGG 6 cut(s) 433, 616, 727, 1012, 1254, 1947
PspPI GGNCC 7 cut(s) 4, 294, 378, 802, 889, 1120, 1580
PspPPI RGGWCCY 1 cut(s) 889
PspXI VCTCGAGB 1 cut(s) 329
PsuI RGATCY 5 cut(s) 311, 532, 996, 1078, 1708
PsyI GACNNNGTC 3 cut(s) 557, 875, 1190
PvuII CAGCTG 1 cut(s) 247
RsaI GTAC 7 cut(s) 962, 986, 1435, 1673, 1940, 2287, 2312
RsaNI GTAC 7 cut(s) 961, 985, 1434, 1672, 1939, 2286, 2311
RseI CAYNNNNRTG 2 cut(s) 545, 1539
SalI GTCGAC 3 cut(s) 41, 626, 1199
SaqAI TTAA 3 cut(s) 36, 1137, 1782
Sau3AI GATC 9 cut(s) 311, 345, 532, 778, 996, 1078, 1182, 1423, 1708
Sau96I GGNCC 7 cut(s) 4, 294, 378, 802, 889, 1120, 1580
ScaI AGTACT 1 cut(s) 1673
SchI GAGTC 2 cut(s) 767, 1909
ScrFI CCNGG 8 cut(s) 183, 435, 618, 729, 1014, 1056, 1256, 1949
SduI GDGCHC 9 cut(s) 237, 647, 675, 725, 1158, 1338, 1409, 2007, 2171
SfaNI GCATC 4 cut(s) 279, 630, 643, 1264
Sfr274I CTCGAG 1 cut(s) 329
SgrAI CRCCGGYG 2 cut(s) 815, 2102
SinI GGWCC 4 cut(s) 294, 889, 1120, 1580
SlaI CTCGAG 1 cut(s) 329
SmiMI CAYNNNNRTG 2 cut(s) 545, 1539
SmlI CTYRAG 2 cut(s) 329, 842
SmoI CTYRAG 2 cut(s) 329, 842
Sse9I AATT 7 cut(s) 10, 1740, 1838, 1905, 2022, 2066, 2154
SspMI CTAG 8 cut(s) 894, 1326, 1563, 1637, 1815, 1961, 2052, 2320
StyD4I CCNGG 8 cut(s) 181, 433, 616, 727, 1012, 1054, 1254, 1947
StyI CCWWGG 6 cut(s) 570, 885, 1357, 1789, 1860, 2276
TaaI ACNGT 7 cut(s) 670, 1050, 1340, 1433, 1603, 1892, 2132
TasI AATT 7 cut(s) 10, 1740, 1838, 1905, 2022, 2066, 2154
TatI WGTACW 3 cut(s) 1671, 1938, 2310
TauI GCSGC 9 cut(s) 179, 209, 225, 324, 414, 684, 815, 1547, 1728
TfiI GAWTC 1 cut(s) 498
Tru1I TTAA 3 cut(s) 36, 1137, 1782
Tru9I TTAA 3 cut(s) 36, 1137, 1782
TseFI GTSAC 7 cut(s) 1050, 1391, 1454, 1595, 1852, 1995, 2038
TseI GCWGC 4 cut(s) 219, 838, 1556, 1665
Tsp45I GTSAC 7 cut(s) 1050, 1391, 1454, 1595, 1852, 1995, 2038
TspDTI ATGAA 8 cut(s) 253, 856, 1164, 1339, 1454, 1489, 2258, 2262
TspGWI ACGGA 2 cut(s) 989, 1165
Tth111I GACNNNGTC 3 cut(s) 557, 875, 1190
Van91I CCANNNNNTGG 3 cut(s) 303, 1216, 2282
VneI GTGCAC 3 cut(s) 643, 671, 1405
VpaK11BI GGWCC 4 cut(s) 294, 889, 1120, 1580
XagI CCTNNNNNAGG 2 cut(s) 332, 1022
XapI RAATTY 2 cut(s) 10, 1740
XceI RCATGY 2 cut(s) 1522, 2095
XcmI CCANNNNNNNNNTGG 2 cut(s) 577, 796
XhoI CTCGAG 1 cut(s) 329
XmiI GTMKAC 3 cut(s) 42, 627, 1200
XmnI GAANNNNTTC 1 cut(s) 1301
XspI CTAG 8 cut(s) 894, 1326, 1563, 1637, 1815, 1961, 2052, 2320
ZraI GACGTC 2 cut(s) 121, 457
ZrmI AGTACT 1 cut(s) 1673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.