FvH4_3g05720

phospho-N-acetylmuramoyl-pentapeptide- transferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008615
Physical Location & Seq
Forward (+)
3339418 .. 3342834
3417 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g05720.t1

Sequence Viewer

Length: 1545 bp
ATGGCATCATCTTATTATTCCTTCAATCTCCGCCACCTCTACCCTCTCCAGCTCCCTCGCCCCCACCGCCGCTCCTCATTTCCCTCCGTCGATCGCCTCCGATCCGGTTCCGGATTCCCTCCGGTATGTTCCGGAATCAGTTACGATCTTAAGTTGATCGGGTTACGAAGCTGTGGACGCCGCTTTGGTCATGGTCCTGTCCACTATGGAGCCTTTGATGTGGATTTTGGTGATTTTTCGCCGGTTGAGGATTGGGGAAATAACCAGGAAGAGACTGCAAACTACATGAGTTTTTCGAGTGATGGTGACGATAGTGATGCCGAGTATGTAGTTTCTCCTCAGTCTGATGTTGATTTGCCTGCTATTACTAAGCCAACTGATGATGCTCTAACTGTTAGTGCTTATCGGCTTGCAATGCTCGGGCGCCAGCGGAAGAGACACAGGATCAGACAAGGAGTACTACTCAACGCAGGACTGATAATGTTCTTACTAGTGGTACTTCTATATGTGGATTGGTGTGCTTGGCGCATTGTTAAACTACCTTTGGCACCCTTCCACTTGACTTGCCCGTTCGTTATATCAGCCGCTTTAGCCTCTTGTGCGGGATATGTTTGTGTTCCACTGCTCTATAGCTTGAAGATTCATCAAATTATCAGAAAAGAAGGTTATGTAAGGCACTCCTTGAAGAAAAGAACTCCCACAATGGGTGGATTGTTCTTTGTACCAGTTGGTGTTACTGTTGCAAGGTTCACTGCCGGTTTTTCTTCTGTTGAAGTAGCAGGAGCAGCTACAGCAACTCTTGCTTTTTCTGCTATTGGATTACTTGATGATATCATTTGCTTGATCAAGAATCATAATAGTGGTCTATCTGCATGGATTAAAATCATTTTGGAGGTAGCTGTTGGAACATGGTTTTCATATTGGTTGCATACGACAAATATATCATCACCCTATGGCATGAAAATGTTGGTTCCTCTACCTGCTCCAATAGGCATAGTGTGGCTGGGAAAATTATATCTAATGCTGGCTTCATTCTGTTTTGTTTCTATGGGAAATGGGGTGGACTTAACTGATGGCCTTGATGGGCTGGCTGGAGGATCTGCTGCATTGGCTTTTATAGGAATGTCAATCGCAGTACTTCCAATATGTTCAGACCTTGCAATATTTGGAGCGTCAATGGCAGGAGCTTGTGTTGGTTTTCTTTTGCACAACAGATACAAGGCATCAGTGTTCATGGGTAATACTGGGTCCTTGGCACTTGGTGGAGGGTTAGCTGCAATGGCTTGTTGCACAGGGATGTTCTTCCCATTGTTCATTTCATCCGGCGTCTTTGTCTTGGAAGCCTCATCAGTTATTTTGCAGGTAATGTACTTTAAGACAACCAAGCACTTGCAAGGTGCGGGTCGCCGGCTCTTCCGAATGGCACCATTTCATCATCACCTTGAACTATGTGGACTTAAAGAACCGGTCATTGTTGGTGGTGCCTATATTATATCGTCCACATTGTCGTTAGCTGCTGCCTATATCGGTCTGATATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00550 ko01100 ko01502 map00550 map01100 map01502
Pfam Domains
Protein Families
Glycos_transf_4 MraY_sig1

Protein Analysis

515

Amino Acids

55.5

Weight (kDa)

8.88

Isoelectric Point (pI)

44.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_4 PF00953 261 - 429 4.5e-23 Glycosyl transferase family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 988, 1351
AccB1I GGYRCC 4 cut(s) 423, 547, 1423, 1481
AccBSI CCGCTC 1 cut(s) 72
AccIII TCCGGA 2 cut(s) 110, 131
AciI CCGC 8 cut(s) 31, 67, 70, 181, 430, 585, 602, 1400
AclWI GGATC 3 cut(s) 96, 452, 1105
AcyI GRCGYC 3 cut(s) 178, 424, 1326
AdeI CACNNNGTG 1 cut(s) 1262
AfaI GTAC 5 cut(s) 459, 498, 723, 1137, 1370
AfiI CCNNNNNNNGG 1 cut(s) 704
AflII CTTAAG 1 cut(s) 149
AgeI ACCGGT 1 cut(s) 1465
AgsI TTSAA 5 cut(s) 25, 637, 685, 773, 1445
AhlI ACTAGT 1 cut(s) 490
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 8 cut(s) 52, 171, 633, 788, 899, 1187, 1274, 1514
AluI AGCT 8 cut(s) 52, 171, 633, 788, 899, 1187, 1274, 1514
Alw26I GTCTC 2 cut(s) 266, 430
AlwI GGATC 3 cut(s) 96, 452, 1105
Ama87I CYCGRG 1 cut(s) 419
Aor13HI TCCGGA 2 cut(s) 110, 131
AoxI GGCC 1 cut(s) 1075
ApeKI GCWGC 5 cut(s) 785, 1103, 1274, 1514, 1517
AsiGI ACCGGT 1 cut(s) 1465
AspLEI GCGC 2 cut(s) 426, 528
AspS9I GGNCC 2 cut(s) 194, 1248
AsuHPI GGTGA 4 cut(s) 242, 317, 939, 1430
AvaI CYCGRG 1 cut(s) 419
AvaII GGWCC 2 cut(s) 194, 1248
BanI GGYRCC 4 cut(s) 423, 547, 1423, 1481
BbvI GCAGC 5 cut(s) 797, 1090, 1261, 1501, 1504
BccI CCATC 3 cut(s) 296, 1067, 1076
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 1 cut(s) 266
BclI TGATCA 1 cut(s) 843
BcoDI GTCTC 2 cut(s) 266, 430
BcuI ACTAGT 1 cut(s) 490
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 2 cut(s) 628, 789
BfoI RGCGCY 1 cut(s) 427
BfrI CTTAAG 1 cut(s) 149
BfuAI ACCTGC 2 cut(s) 988, 1351
BisI GCNGC 8 cut(s) 70, 181, 585, 786, 1104, 1275, 1515, 1518
BlsI GCNGC 8 cut(s) 71, 182, 586, 787, 1105, 1276, 1516, 1519
BmcAI AGTACT 2 cut(s) 459, 1137
Bme1390I CCNGG 1 cut(s) 266
Bme18I GGWCC 2 cut(s) 194, 1248
BmeT110I CYCGRG 1 cut(s) 419
BmgT120I GGNCC 2 cut(s) 194, 1248
BmiI GGNNCC 8 cut(s) 109, 211, 425, 549, 972, 1249, 1425, 1483
BmrFI CCNGG 1 cut(s) 266
BmrI ACTGGG 1 cut(s) 1254
BmsI GCATC 4 cut(s) 14, 307, 373, 1232
BmuI ACTGGG 1 cut(s) 1254
BplI GAGNNNNNCTC 2 cut(s) 447, 479
BpmI CTGGAG 2 cut(s) 32, 1113
BsaBI GATNNNNATC 1 cut(s) 881
BsaHI GRCGYC 3 cut(s) 178, 424, 1326
BsaJI CCNNGG 1 cut(s) 1251
BsaWI WCCGGW 5 cut(s) 104, 110, 121, 131, 1465
BsaXI ACNNNNNCTCC 4 cut(s) 56, 86, 1176, 1206
Bsc4I CCNNNNNNNGG 1 cut(s) 704
Bse118I RCCGGY 4 cut(s) 241, 755, 1407, 1465
Bse1I ACTGG 2 cut(s) 725, 1249
Bse3DI GCAATG 2 cut(s) 420, 1284
Bse8I GATNNNNATC 1 cut(s) 881
BseAI TCCGGA 2 cut(s) 110, 131
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 1251
BseGI GGATG 2 cut(s) 1302, 1319
BseJI GATNNNNATC 1 cut(s) 881
BseLI CCNNNNNNNGG 1 cut(s) 704
BseMI GCAATG 2 cut(s) 420, 1284
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 2 cut(s) 725, 1249
BseRI GAGGAG 2 cut(s) 64, 327
BseXI GCAGC 5 cut(s) 797, 1090, 1261, 1501, 1504
BseYI CCCAGC 1 cut(s) 1003
Bsh1285I CGRYCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 1077
BshNI GGYRCC 4 cut(s) 423, 547, 1423, 1481
BshTI ACCGGT 1 cut(s) 1465
BsiEI CGRYCG 1 cut(s) 94
BsiHKCI CYCGRG 1 cut(s) 419
BsiSI CCGG 9 cut(s) 105, 111, 122, 132, 242, 756, 1323, 1408, 1466
BslI CCNNNNNNNGG 1 cut(s) 704
BsmAI GTCTC 2 cut(s) 266, 430
BsnI GGCC 1 cut(s) 1077
BsoBI CYCGRG 1 cut(s) 419
Bsp13I TCCGGA 2 cut(s) 110, 131
Bsp143I GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
BspACI CCGC 8 cut(s) 31, 67, 70, 181, 430, 585, 602, 1400
BspANI GGCC 1 cut(s) 1077
BspCNI CTCAG 1 cut(s) 352
BspEI TCCGGA 2 cut(s) 110, 131
BspLI GGNNCC 8 cut(s) 109, 211, 425, 549, 972, 1249, 1425, 1483
BspMI ACCTGC 2 cut(s) 988, 1351
BspPI GGATC 3 cut(s) 96, 452, 1105
BspQI GCTCTTC 1 cut(s) 1418
BspT107I GGYRCC 4 cut(s) 423, 547, 1423, 1481
BspTI CTTAAG 1 cut(s) 149
BsrBI CCGCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 420, 1284
BsrFI RCCGGY 4 cut(s) 241, 755, 1407, 1465
BsrI ACTGG 2 cut(s) 725, 1249
BssAI RCCGGY 4 cut(s) 241, 755, 1407, 1465
BssECI CCNNGG 1 cut(s) 1251
BssMI GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
BssNI GRCGYC 3 cut(s) 178, 424, 1326
BssT1I CCWWGG 1 cut(s) 1251
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 2 cut(s) 394, 739
Bst6I CTCTTC 3 cut(s) 264, 428, 1418
BstACI GRCGYC 3 cut(s) 178, 424, 1326
BstAFI CTTAAG 1 cut(s) 149
BstAPI GCANNNNNTGC 1 cut(s) 800
BstC8I GCNNGC 6 cut(s) 360, 411, 428, 1026, 1089, 1409
BstDEI CTNAG 3 cut(s) 339, 369, 1542
BstF5I GGATG 2 cut(s) 1302, 1319
BstH2I RGCGCY 1 cut(s) 427
BstHHI GCGC 2 cut(s) 426, 528
BstKTI GATC 7 cut(s) 94, 104, 148, 159, 447, 846, 1100
BstMAI GTCTC 2 cut(s) 266, 430
BstMBI GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
BstMCI CGRYCG 1 cut(s) 94
BstNI CCWGG 1 cut(s) 266
BstSCI CCNGG 1 cut(s) 264
BstSFI CTRYAG 2 cut(s) 628, 789
BstV1I GCAGC 5 cut(s) 797, 1090, 1261, 1501, 1504
BstX2I RGATCY 1 cut(s) 1097
BstYI RGATCY 1 cut(s) 1097
BsuRI GGCC 1 cut(s) 1077
BtsCI GGATG 2 cut(s) 1302, 1319
BtsI GCAGTG 2 cut(s) 620, 750
BtsIMutI CAGTG 3 cut(s) 620, 750, 1233
BveI ACCTGC 2 cut(s) 988, 1351
Cac8I GCNNGC 6 cut(s) 360, 411, 428, 1026, 1089, 1409
CfoI GCGC 2 cut(s) 426, 528
Cfr10I RCCGGY 4 cut(s) 241, 755, 1407, 1465
Cfr13I GGNCC 2 cut(s) 194, 1248
CseI GACGC 3 cut(s) 186, 1161, 1315
Csp6I GTAC 5 cut(s) 458, 497, 722, 1136, 1369
CspAI ACCGGT 1 cut(s) 1465
CspCI CAANNNNNGTGG 2 cut(s) 545, 580
CviAII CATG 6 cut(s) 191, 286, 873, 909, 958, 1234
CviQI GTAC 5 cut(s) 458, 497, 722, 1136, 1369
DdeI CTNAG 3 cut(s) 339, 369, 1542
DinI GGCGCC 1 cut(s) 425
DpnI GATC 7 cut(s) 93, 103, 147, 158, 446, 845, 1099
DpnII GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
DraIII CACNNNGTG 1 cut(s) 1262
Eam1104I CTCTTC 3 cut(s) 264, 428, 1418
EarI CTCTTC 3 cut(s) 264, 428, 1418
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 1 cut(s) 1251
Eco32I GATATC 2 cut(s) 832, 1536
Eco47I GGWCC 2 cut(s) 194, 1248
Eco88I CYCGRG 1 cut(s) 419
EcoO109I RGGNCCY 1 cut(s) 1248
EcoRII CCWGG 1 cut(s) 264
EcoRV GATATC 2 cut(s) 832, 1536
EcoT14I CCWWGG 1 cut(s) 1251
EgeI GGCGCC 1 cut(s) 425
EheI GGCGCC 1 cut(s) 425
ErhI CCWWGG 1 cut(s) 1251
FaeI CATG 6 cut(s) 194, 289, 876, 912, 961, 1237
FatI CATG 6 cut(s) 190, 285, 872, 908, 957, 1233
FauI CCCGC 2 cut(s) 595, 1393
FbaI TGATCA 1 cut(s) 843
Fnu4HI GCNGC 8 cut(s) 70, 181, 585, 786, 1104, 1275, 1515, 1518
FokI GGATG 2 cut(s) 1306, 1309
Fsp4HI GCNGC 8 cut(s) 70, 181, 585, 786, 1104, 1275, 1515, 1518
FspBI CTAG 1 cut(s) 491
GlaI GCGC 2 cut(s) 425, 527
GluI GCNGC 8 cut(s) 70, 181, 585, 786, 1104, 1275, 1515, 1518
GsaI CCCAGC 1 cut(s) 1007
GsuI CTGGAG 2 cut(s) 32, 1113
HaeII RGCGCY 1 cut(s) 427
HaeIII GGCC 1 cut(s) 1077
HapII CCGG 9 cut(s) 105, 111, 122, 132, 242, 756, 1323, 1408, 1466
HgaI GACGC 3 cut(s) 186, 1161, 1315
HhaI GCGC 2 cut(s) 426, 528
Hin1I GRCGYC 3 cut(s) 178, 424, 1326
Hin1II CATG 6 cut(s) 194, 289, 876, 912, 961, 1237
Hin6I GCGC 2 cut(s) 424, 526
HinP1I GCGC 2 cut(s) 424, 526
HinfI GANTC 4 cut(s) 114, 135, 640, 850
HpaII CCGG 9 cut(s) 105, 111, 122, 132, 242, 756, 1323, 1408, 1466
HphI GGTGA 4 cut(s) 242, 317, 939, 1430
Hpy166II GTNNAC 6 cut(s) 176, 202, 750, 1063, 1454, 1500
Hpy188I TCNGA 7 cut(s) 101, 346, 449, 656, 1153, 1418, 1533
Hpy188III TCNNGA 3 cut(s) 111, 132, 847
Hpy8I GTNNAC 6 cut(s) 176, 202, 750, 1063, 1454, 1500
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 3 cut(s) 31, 562, 656
HpyCH4III ACNGT 2 cut(s) 394, 739
HpyF3I CTNAG 3 cut(s) 339, 369, 1542
Hsp92I GRCGYC 3 cut(s) 178, 424, 1326
Hsp92II CATG 6 cut(s) 194, 289, 876, 912, 961, 1237
HspAI GCGC 2 cut(s) 424, 526
KasI GGCGCC 1 cut(s) 423
Kpn2I TCCGGA 2 cut(s) 110, 131
KroI GCCGGC 1 cut(s) 1407
KroNI GCCGGC 1 cut(s) 1409
Ksp22I TGATCA 1 cut(s) 843
Kzo9I GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
LguI GCTCTTC 1 cut(s) 1418
LmnI GCTCC 7 cut(s) 57, 77, 209, 782, 988, 1169, 1184
Lsp1109I GCAGC 5 cut(s) 797, 1090, 1261, 1501, 1504
LweI GCATC 4 cut(s) 14, 307, 373, 1232
MaeI CTAG 1 cut(s) 491
MaeIII GTNAC 4 cut(s) 140, 162, 305, 733
MalI GATC 7 cut(s) 93, 103, 147, 158, 446, 845, 1099
MbiI CCGCTC 1 cut(s) 72
MboI GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
MboII GAAGA 7 cut(s) 281, 445, 649, 697, 756, 1294, 1405
MflI RGATCY 1 cut(s) 1097
MluCI AATT 2 cut(s) 648, 1010
Mly113I GGCGCC 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 883
MroI TCCGGA 2 cut(s) 110, 131
MroNI GCCGGC 1 cut(s) 1407
MseI TTAA 6 cut(s) 150, 534, 879, 1067, 1374, 1458
MslI CAYNNNNRTG 3 cut(s) 858, 962, 1295
MspA1I CMGCKG 1 cut(s) 430
MspCI CTTAAG 1 cut(s) 149
MspI CCGG 9 cut(s) 105, 111, 122, 132, 242, 756, 1323, 1408, 1466
MspR9I CCNGG 1 cut(s) 266
MvaI CCWGG 1 cut(s) 266
NaeI GCCGGC 1 cut(s) 1409
NarI GGCGCC 1 cut(s) 424
NdeII GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
NgoMIV GCCGGC 1 cut(s) 1407
NlaIII CATG 6 cut(s) 194, 289, 876, 912, 961, 1237
NlaIV GGNNCC 8 cut(s) 109, 211, 425, 549, 972, 1249, 1425, 1483
NmeAIII GCCGAG 1 cut(s) 346
NmuCI GTSAC 1 cut(s) 305
PciSI GCTCTTC 1 cut(s) 1418
PdiI GCCGGC 1 cut(s) 1409
PfeI GAWTC 4 cut(s) 114, 135, 640, 850
PinAI ACCGGT 1 cut(s) 1465
PkrI GCNGC 8 cut(s) 71, 182, 586, 787, 1105, 1276, 1516, 1519
Ple19I CGATCG 1 cut(s) 94
PluTI GGCGCC 1 cut(s) 427
PpuMI RGGWCCY 1 cut(s) 1248
Psp5II RGGWCCY 1 cut(s) 1248
Psp6I CCWGG 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 1003
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 8 cut(s) 109, 211, 425, 549, 972, 1249, 1425, 1483
PspPI GGNCC 2 cut(s) 194, 1248
PspPPI RGGWCCY 1 cut(s) 1248
PsuI RGATCY 1 cut(s) 1097
PvuI CGATCG 1 cut(s) 94
RsaI GTAC 5 cut(s) 459, 498, 723, 1137, 1370
RsaNI GTAC 5 cut(s) 458, 497, 722, 1136, 1369
RseI CAYNNNNRTG 3 cut(s) 858, 962, 1295
SapI GCTCTTC 1 cut(s) 1418
SaqAI TTAA 6 cut(s) 150, 534, 879, 1067, 1374, 1458
SatI GCNGC 8 cut(s) 70, 181, 585, 786, 1104, 1275, 1515, 1518
Sau3AI GATC 7 cut(s) 91, 101, 145, 156, 444, 843, 1097
Sau96I GGNCC 2 cut(s) 194, 1248
ScaI AGTACT 2 cut(s) 459, 1137
ScrFI CCNGG 1 cut(s) 266
SfaNI GCATC 4 cut(s) 14, 307, 373, 1232
SfcI CTRYAG 2 cut(s) 628, 789
SfoI GGCGCC 1 cut(s) 425
SinI GGWCC 2 cut(s) 194, 1248
SmiMI CAYNNNNRTG 3 cut(s) 858, 962, 1295
SmlI CTYRAG 1 cut(s) 149
SmoI CTYRAG 1 cut(s) 149
SpeI ACTAGT 1 cut(s) 490
Sse9I AATT 2 cut(s) 648, 1010
SsiI CCGC 8 cut(s) 31, 67, 70, 181, 430, 585, 602, 1400
SspDI GGCGCC 1 cut(s) 423
SspI AATATT 1 cut(s) 1164
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 1251
TaaI ACNGT 2 cut(s) 394, 739
TaqI TCGA 2 cut(s) 90, 296
TaqII GACCGA 1 cut(s) 1517
TasI AATT 2 cut(s) 648, 1010
TatI WGTACW 3 cut(s) 457, 1135, 1368
TauI GCSGC 3 cut(s) 72, 183, 587
TfiI GAWTC 4 cut(s) 114, 135, 640, 850
Tru1I TTAA 6 cut(s) 150, 534, 879, 1067, 1374, 1458
Tru9I TTAA 6 cut(s) 150, 534, 879, 1067, 1374, 1458
TscAI CASTG 3 cut(s) 627, 757, 1233
TseFI GTSAC 1 cut(s) 305
TseI GCWGC 5 cut(s) 785, 1103, 1274, 1514, 1517
Tsp45I GTSAC 1 cut(s) 305
TspDTI ATGAA 8 cut(s) 632, 906, 974, 1020, 1222, 1303, 1308, 1421
TspGWI ACGGA 1 cut(s) 76
TspRI CASTG 3 cut(s) 627, 757, 1233
Vha464I CTTAAG 1 cut(s) 149
VpaK11BI GGWCC 2 cut(s) 194, 1248
XspI CTAG 1 cut(s) 491
ZrmI AGTACT 2 cut(s) 459, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.