RchiOBHm_Chr5g0013951

phospho-N-acetylmuramoyl-pentapeptide- transferase homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008615
Physical Location & Seq
Reverse (-)
9453668 .. 9457805
4138 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29444

Sequence Viewer

Length: 1551 bp
ATGGCATCTCACTCCCTCAATCTTCGCCACCTCTACCCTCTCCAGCTCCCTCGCCCCCACCGCCGCTCCTCATTTCGCGCCGTCGATCGCCTCCGATCCTGTTCCGGCTTCTCGCCGGTACGTTCCGGAATCAGTTACGATCTTAAGTTGATTGGGTTGCGAAGAGGTGGATGCCGCTTTGGTCATGGTCGTGTTCGTTATGGAGCCTTTGATGTGGATTTTGGTGATGTTTCGCCGGTTGAGGATTGGGGTAATAACCAGGAAGAGACTGCAGAACACGTGAGTTTTTCCAGCGATGGTGACGATAGTGATGCCGAGTTTGTAGTGTCTCCTCAGTCTGATATTGATTTGCCTGCTATTACTAAACAAACTGACGATGCTCTAACTGTGAGTGCTTATCGGCTTGCGATGCTTGGGCATAAGCGCAAGAAACACAGGATCAGACAAGGAGTATTACTCAACACGGGACTGATAATGTTCCTGGTAGTGGTGCTTCTATATGTGGATTGGTGTGCTTGGCGGATTGTTAGACTACCTTTGGCACCCTTCCACTTGACTTGCCCCTTCGTTATATCAGCCATTTTGGCCTCTTGTGCAGGATATGTTTGTGTTCCACTGCTCTATAGCTTGAAGATTCATCAAATTATCAGAAAAGAAGGTCATGTAAGGCACTCCTTGAAAAAAAGAACTCCCACAATGGGTGGACTGTTCTTTGTACCAATTGGTGTCATTGTCGCAAGGTTCACTTCTGGTTTTTCTTCCGTTGAAGTGTGTGGGGCAGCTACAGCAACTCTTGCATTTGCTGCTATTGGACTACTTGATGATATCATATGCTTGATAAAGAATCAGAATAGTGGTCTATCTGCATGGATAAAAATTATTTTGGAGGTAGCTGTTGGAACCTGGTTTTCGTATTGGTTGCATACAACAAATATATCATCACCCTATGGCATGTATATCTTCTTGAAAATGTTGGTTCCTCTACCTTCTCCACTAGGCATTGTTTGGCTGGGAAAACTATATTTAATGCTGGCTTCATTCTGTTTTGTTTCTATGGGAAATGGGGTGAACTTAACAGATGGTCTTGATGGGCTGGCTGGAGGATCTGCTGCATTGGCTTTTATAGGAATGTCGATCGCAGTGCTTCCAATATGTTCAGACCTTGCGATATTTGGAGCGTCAATGGCAGGAGCTTGTGTTGGTTTTCTTTTGCACAACCGATATAAGGCATCAGTGTTCATGGGTGATACTGGATCCTTGGCAATTGGTGGAGGGTTAGCTGCAATGGCTTGTTGCACGGGGATGTTCTTCCCATTGTTCATTTCATCTGGCATCTTTGTCTTGGAAGCCTCATCAGTTATTTTGCAGGTTATGTACTTTAAGACAACCAAGCGCTTGCAAGGTGCGGGTCGTCGGCTCTTCCGAATGGCACCCTTTCATCATCACCTTGAACTATGTGGACTTAAAGAACCAATCATTGTTGGTGGTGCCTATATAATATCGTCCACATTATCGTTAATTGCTGCCTATGTCGGTCTGATATCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00550 ko01100 ko01502 map00550 map01100 map01502
Pfam Domains
Protein Families
Glycos_transf_4 MraY_sig1

Protein Analysis

516

Amino Acids

56.0

Weight (kDa)

9.14

Isoelectric Point (pI)

41.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycos_transf_4 PF00953 260 - 431 3.1e-25 Glycosyl transferase family 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1357
AccB1I GGYRCC 3 cut(s) 541, 1429, 1487
AccBSI CCGCTC 1 cut(s) 66
AccII CGCG 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 125
AciI CCGC 5 cut(s) 61, 64, 175, 520, 1406
AclWI GGATC 5 cut(s) 90, 446, 1111, 1248, 1261
AcvI CACGTG 1 cut(s) 280
AfaI GTAC 3 cut(s) 120, 717, 1376
AfeI AGCGCT 1 cut(s) 1394
AfiI CCNNNNNNNGG 3 cut(s) 487, 698, 1225
AflII CTTAAG 1 cut(s) 143
AflIII ACRYGT 1 cut(s) 277
AgsI TTSAA 5 cut(s) 631, 679, 767, 967, 1451
AjnI CCWGG 3 cut(s) 258, 480, 902
AluBI AGCT 6 cut(s) 46, 627, 782, 893, 1193, 1280
AluI AGCT 6 cut(s) 46, 627, 782, 893, 1193, 1280
Alw26I GTCTC 2 cut(s) 260, 333
AlwI GGATC 5 cut(s) 90, 446, 1111, 1248, 1261
Aor13HI TCCGGA 1 cut(s) 125
Aor51HI AGCGCT 1 cut(s) 1394
AoxI GGCC 1 cut(s) 585
ApeKI GCWGC 5 cut(s) 779, 803, 1109, 1280, 1523
AspLEI GCGC 3 cut(s) 80, 426, 1395
AsuHPI GGTGA 6 cut(s) 236, 311, 933, 1078, 1256, 1436
BamHI GGATCC 1 cut(s) 1253
BanI GGYRCC 3 cut(s) 541, 1429, 1487
BarI GAAGNNNNNNTAC 2 cut(s) 477, 509
BbrPI CACGTG 1 cut(s) 280
BbvI GCAGC 5 cut(s) 790, 791, 1096, 1267, 1510
BccI CCATC 3 cut(s) 290, 1073, 1082
BceAI ACGGC 1 cut(s) 65
BcgI CGANNNNNNTGC 4 cut(s) 293, 327, 1123, 1157
BciT130I CCWGG 3 cut(s) 260, 482, 904
BcoDI GTCTC 2 cut(s) 260, 333
BfaI CTAG 1 cut(s) 995
BfmI CTRYAG 3 cut(s) 270, 622, 783
BfoI RGCGCY 1 cut(s) 1396
BfrI CTTAAG 1 cut(s) 143
BfuAI ACCTGC 1 cut(s) 1357
BglI GCCNNNNNGGC 1 cut(s) 584
BisI GCNGC 7 cut(s) 64, 175, 780, 804, 1110, 1281, 1524
BlsI GCNGC 7 cut(s) 65, 176, 781, 805, 1111, 1282, 1525
Bme1390I CCNGG 3 cut(s) 260, 482, 904
BmiI GGNNCC 7 cut(s) 205, 543, 901, 978, 1255, 1431, 1489
BmrFI CCNGG 3 cut(s) 260, 482, 904
BmsI GCATC 7 cut(s) 14, 161, 301, 367, 399, 1238, 1341
BplI GAGNNNNNCTC 2 cut(s) 441, 473
BpmI CTGGAG 2 cut(s) 26, 1119
BsaAI YACGTR 1 cut(s) 280
BsaJI CCNNGG 1 cut(s) 1257
BsaWI WCCGGW 1 cut(s) 125
BsaXI ACNNNNNCTCC 4 cut(s) 50, 80, 1182, 1212
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 698, 1225
Bse118I RCCGGY 2 cut(s) 115, 235
Bse1I ACTGG 1 cut(s) 1255
Bse3DI GCAATG 1 cut(s) 1290
BseAI TCCGGA 1 cut(s) 125
BseBI CCWGG 3 cut(s) 260, 482, 904
BseDI CCNNGG 1 cut(s) 1257
BseGI GGATG 2 cut(s) 176, 1308
BseLI CCNNNNNNNGG 3 cut(s) 487, 698, 1225
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 1 cut(s) 347
BseNI ACTGG 1 cut(s) 1255
BseRI GAGGAG 2 cut(s) 58, 321
BseXI GCAGC 5 cut(s) 790, 791, 1096, 1267, 1510
BseYI CCCAGC 1 cut(s) 1009
BsgI GTGCAG 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 78
Bsh1285I CGRYCG 2 cut(s) 88, 1137
BshFI GGCC 1 cut(s) 587
BshNI GGYRCC 3 cut(s) 541, 1429, 1487
BsiEI CGRYCG 2 cut(s) 88, 1137
BsiSI CCGG 4 cut(s) 105, 116, 126, 236
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 3 cut(s) 487, 698, 1225
BsmAI GTCTC 2 cut(s) 260, 333
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 1 cut(s) 587
Bsp13I TCCGGA 1 cut(s) 125
Bsp143I GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
BspACI CCGC 5 cut(s) 61, 64, 175, 520, 1406
BspANI GGCC 1 cut(s) 587
BspCNI CTCAG 1 cut(s) 346
BspEI TCCGGA 1 cut(s) 125
BspFNI CGCG 1 cut(s) 78
BspLI GGNNCC 7 cut(s) 205, 543, 901, 978, 1255, 1431, 1489
BspMAI CTGCAG 1 cut(s) 274
BspMI ACCTGC 1 cut(s) 1357
BspPI GGATC 5 cut(s) 90, 446, 1111, 1248, 1261
BspQI GCTCTTC 1 cut(s) 1424
BspT107I GGYRCC 3 cut(s) 541, 1429, 1487
BspTI CTTAAG 1 cut(s) 143
BsrBI CCGCTC 1 cut(s) 66
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 2 cut(s) 115, 235
BsrI ACTGG 1 cut(s) 1255
BssAI RCCGGY 2 cut(s) 115, 235
BssECI CCNNGG 1 cut(s) 1257
BssMI GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
BssT1I CCWWGG 1 cut(s) 1257
Bst2UI CCWGG 3 cut(s) 260, 482, 904
Bst4CI ACNGT 2 cut(s) 388, 708
Bst6I CTCTTC 3 cut(s) 157, 258, 1424
BstAFI CTTAAG 1 cut(s) 143
BstAPI GCANNNNNTGC 2 cut(s) 794, 803
BstBAI YACGTR 1 cut(s) 280
BstC8I GCNNGC 5 cut(s) 354, 405, 1032, 1095, 1397
BstDEI CTNAG 2 cut(s) 333, 1548
BstF5I GGATG 2 cut(s) 176, 1308
BstFNI CGCG 1 cut(s) 78
BstH2I RGCGCY 1 cut(s) 1396
BstHHI GCGC 3 cut(s) 80, 426, 1395
BstKTI GATC 7 cut(s) 88, 98, 142, 441, 1106, 1137, 1256
BstMAI GTCTC 2 cut(s) 260, 333
BstMBI GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
BstMCI CGRYCG 2 cut(s) 88, 1137
BstNI CCWGG 3 cut(s) 260, 482, 904
BstNSI RCATGY 1 cut(s) 955
BstSCI CCNGG 3 cut(s) 258, 480, 902
BstSFI CTRYAG 3 cut(s) 270, 622, 783
BstUI CGCG 1 cut(s) 78
BstV1I GCAGC 5 cut(s) 790, 791, 1096, 1267, 1510
BstX2I RGATCY 2 cut(s) 1103, 1253
BstYI RGATCY 2 cut(s) 1103, 1253
BsuRI GGCC 1 cut(s) 587
BtgZI GCGATG 2 cut(s) 309, 422
BtsCI GGATG 2 cut(s) 176, 1308
BtsI GCAGTG 2 cut(s) 614, 1146
BtsIMutI CAGTG 3 cut(s) 614, 1146, 1239
BveI ACCTGC 1 cut(s) 1357
Cac8I GCNNGC 5 cut(s) 354, 405, 1032, 1095, 1397
CfoI GCGC 3 cut(s) 80, 426, 1395
Cfr10I RCCGGY 2 cut(s) 115, 235
CseI GACGC 1 cut(s) 1167
CsiI ACCWGGT 1 cut(s) 902
Csp6I GTAC 3 cut(s) 119, 716, 1375
CspCI CAANNNNNGTGG 2 cut(s) 539, 574
CviAII CATG 5 cut(s) 185, 662, 867, 952, 1240
CviQI GTAC 3 cut(s) 119, 716, 1375
DdeI CTNAG 2 cut(s) 333, 1548
DpnI GATC 7 cut(s) 87, 97, 141, 440, 1105, 1136, 1255
DpnII GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
Eam1104I CTCTTC 3 cut(s) 157, 258, 1424
EarI CTCTTC 3 cut(s) 157, 258, 1424
EciI GGCGGA 1 cut(s) 535
Eco130I CCWWGG 1 cut(s) 1257
Eco32I GATATC 2 cut(s) 826, 1542
Eco47III AGCGCT 1 cut(s) 1394
Eco72I CACGTG 1 cut(s) 280
EcoRII CCWGG 3 cut(s) 258, 480, 902
EcoRV GATATC 2 cut(s) 826, 1542
EcoT14I CCWWGG 1 cut(s) 1257
ErhI CCWWGG 1 cut(s) 1257
FaeI CATG 5 cut(s) 188, 665, 870, 955, 1243
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FaqI GGGAC 1 cut(s) 480
FatI CATG 5 cut(s) 184, 661, 866, 951, 1239
FauI CCCGC 1 cut(s) 1399
FauNDI CATATG 1 cut(s) 830
Fnu4HI GCNGC 7 cut(s) 64, 175, 780, 804, 1110, 1281, 1524
FokI GGATG 2 cut(s) 183, 1315
Fsp4HI GCNGC 7 cut(s) 64, 175, 780, 804, 1110, 1281, 1524
FspBI CTAG 1 cut(s) 995
GlaI GCGC 3 cut(s) 79, 425, 1394
GluI GCNGC 7 cut(s) 64, 175, 780, 804, 1110, 1281, 1524
GsaI CCCAGC 1 cut(s) 1013
GsuI CTGGAG 2 cut(s) 26, 1119
HaeII RGCGCY 1 cut(s) 1396
HaeIII GGCC 1 cut(s) 587
HapII CCGG 4 cut(s) 105, 116, 126, 236
HgaI GACGC 1 cut(s) 1167
HhaI GCGC 3 cut(s) 80, 426, 1395
Hin1II CATG 5 cut(s) 188, 665, 870, 955, 1243
Hin6I GCGC 3 cut(s) 78, 424, 1393
HinP1I GCGC 3 cut(s) 78, 424, 1393
HinfI GANTC 3 cut(s) 129, 634, 844
HpaII CCGG 4 cut(s) 105, 116, 126, 236
HphI GGTGA 6 cut(s) 236, 311, 933, 1078, 1256, 1436
Hpy166II GTNNAC 5 cut(s) 704, 744, 1069, 1460, 1506
Hpy188I TCNGA 8 cut(s) 95, 340, 443, 650, 849, 1159, 1424, 1539
Hpy188III TCNNGA 3 cut(s) 126, 964, 1085
Hpy8I GTNNAC 5 cut(s) 704, 744, 1069, 1460, 1506
Hpy99I CGWCG 2 cut(s) 86, 1416
HpyAV CCTTC 4 cut(s) 556, 574, 650, 996
HpyCH4III ACNGT 2 cut(s) 388, 708
HpyCH4IV ACGT 2 cut(s) 121, 279
HpyF3I CTNAG 2 cut(s) 333, 1548
HpySE526I ACGT 2 cut(s) 121, 279
Hsp92II CATG 5 cut(s) 188, 665, 870, 955, 1243
HspAI GCGC 3 cut(s) 78, 424, 1393
Kpn2I TCCGGA 1 cut(s) 125
Kzo9I GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
LguI GCTCTTC 1 cut(s) 1424
LmnI GCTCC 5 cut(s) 51, 71, 203, 1175, 1190
Lsp1109I GCAGC 5 cut(s) 790, 791, 1096, 1267, 1510
LweI GCATC 7 cut(s) 14, 161, 301, 367, 399, 1238, 1341
MabI ACCWGGT 1 cut(s) 902
MaeI CTAG 1 cut(s) 995
MaeII ACGT 2 cut(s) 121, 279
MaeIII GTNAC 2 cut(s) 134, 299
MalI GATC 7 cut(s) 87, 97, 141, 440, 1105, 1136, 1255
MbiI CCGCTC 1 cut(s) 66
MboI GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
MboII GAAGA 8 cut(s) 14, 174, 275, 643, 750, 952, 1300, 1411
MfeI CAATTG 2 cut(s) 720, 1263
MflI RGATCY 2 cut(s) 1103, 1253
MluCI AATT 5 cut(s) 642, 720, 876, 1263, 1518
MmeI TCCRAC 1 cut(s) 877
MroI TCCGGA 1 cut(s) 125
MseI TTAA 6 cut(s) 144, 1025, 1073, 1380, 1464, 1517
MslI CAYNNNNRTG 2 cut(s) 189, 1301
MspCI CTTAAG 1 cut(s) 143
MspI CCGG 4 cut(s) 105, 116, 126, 236
MspR9I CCNGG 3 cut(s) 260, 482, 904
MunI CAATTG 2 cut(s) 720, 1263
MvaI CCWGG 3 cut(s) 260, 482, 904
MvnI CGCG 1 cut(s) 78
NdeI CATATG 1 cut(s) 830
NdeII GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
NlaIII CATG 5 cut(s) 188, 665, 870, 955, 1243
NlaIV GGNNCC 7 cut(s) 205, 543, 901, 978, 1255, 1431, 1489
NmeAIII GCCGAG 1 cut(s) 340
NmuCI GTSAC 1 cut(s) 299
NspI RCATGY 1 cut(s) 955
PciSI GCTCTTC 1 cut(s) 1424
PcsI WCGNNNNNNNCGW 1 cut(s) 1420
PfeI GAWTC 3 cut(s) 129, 634, 844
PkrI GCNGC 7 cut(s) 65, 176, 781, 805, 1111, 1282, 1525
Ple19I CGATCG 2 cut(s) 88, 1137
PmaCI CACGTG 1 cut(s) 280
PmlI CACGTG 1 cut(s) 280
Ppu21I YACGTR 1 cut(s) 280
Psp6I CCWGG 3 cut(s) 258, 480, 902
PspCI CACGTG 1 cut(s) 280
PspFI CCCAGC 1 cut(s) 1009
PspGI CCWGG 3 cut(s) 258, 480, 902
PspN4I GGNNCC 7 cut(s) 205, 543, 901, 978, 1255, 1431, 1489
PstI CTGCAG 1 cut(s) 274
PsuI RGATCY 2 cut(s) 1103, 1253
PvuI CGATCG 2 cut(s) 88, 1137
RsaI GTAC 3 cut(s) 120, 717, 1376
RsaNI GTAC 3 cut(s) 119, 716, 1375
RseI CAYNNNNRTG 2 cut(s) 189, 1301
SapI GCTCTTC 1 cut(s) 1424
SaqAI TTAA 6 cut(s) 144, 1025, 1073, 1380, 1464, 1517
SatI GCNGC 7 cut(s) 64, 175, 780, 804, 1110, 1281, 1524
Sau3AI GATC 7 cut(s) 85, 95, 139, 438, 1103, 1134, 1253
ScrFI CCNGG 3 cut(s) 260, 482, 904
SexAI ACCWGGT 1 cut(s) 902
SfaNI GCATC 7 cut(s) 14, 161, 301, 367, 399, 1238, 1341
SfcI CTRYAG 3 cut(s) 270, 622, 783
SmiMI CAYNNNNRTG 2 cut(s) 189, 1301
SmlI CTYRAG 1 cut(s) 143
SmoI CTYRAG 1 cut(s) 143
Sse9I AATT 5 cut(s) 642, 720, 876, 1263, 1518
SsiI CCGC 5 cut(s) 61, 64, 175, 520, 1406
SspMI CTAG 1 cut(s) 995
StyD4I CCNGG 3 cut(s) 258, 480, 902
StyI CCWWGG 1 cut(s) 1257
TaaI ACNGT 2 cut(s) 388, 708
TaiI ACGT 2 cut(s) 124, 282
TaqI TCGA 2 cut(s) 84, 1133
TaqII GACCGA 1 cut(s) 1523
TasI AATT 5 cut(s) 642, 720, 876, 1263, 1518
TatI WGTACW 1 cut(s) 1374
TauI GCSGC 2 cut(s) 66, 177
TfiI GAWTC 3 cut(s) 129, 634, 844
Tru1I TTAA 6 cut(s) 144, 1025, 1073, 1380, 1464, 1517
Tru9I TTAA 6 cut(s) 144, 1025, 1073, 1380, 1464, 1517
TscAI CASTG 3 cut(s) 621, 1146, 1239
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 5 cut(s) 779, 803, 1109, 1280, 1523
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 6 cut(s) 626, 1026, 1228, 1309, 1314, 1427
TspGWI ACGGA 1 cut(s) 751
TspRI CASTG 3 cut(s) 621, 1146, 1239
Vha464I CTTAAG 1 cut(s) 143
XceI RCATGY 1 cut(s) 955
XspI CTAG 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.