FvH4_3g09040

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012982
Physical Location & Seq
Forward (+)
5284446 .. 5286073
1628 bp
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UTR
Exon/CDS
Intron
FvH4_3g09040.t1

Sequence Viewer

Length: 831 bp
ATGGGTTTTGCAAAAGCTCTAGTTTTTGTTCAGCTCTCTATTCTCCTCCTCAACTCCTTCACCGTTCTTGCTGACTTCTCCGCTATCGAAGCTCAGTCACCGGTGCCGCCGCGCCACCACGGCTACTATGCACACCCAATAGCCGCCCCTGCCCACCCTCCCACTCACGGCCACCACCACCACCACCCCAAACCTCACCCTCACCCTCCAACCGTCGCCCCGGTTCACCCTCCGGCTCACGCTCCTGCCCACCCTCCCTCTCACCACCACCACCACGCTCACCCACCTTCCGTCGCCCCGGCTCACTCTCCTTACCACCACGCTCCTGTTAAGCCTCCAAGCCACCCTCCTGTTAAGCCCCCGAGCCACCCTCCTGTTCACCCCGCACCACCTGCTCACGCTCCTGCCCCAGTTCACCCTCCTAAGAAGCCCTTCCCTCGTAGCTTCGTCGCCGTTCAAGGCGTCGTCTACTGCAAGTCTTGCAAGTACTCCGGCGTCGACACTCTCCTCAACGCCACCGCCATCGCCGGTGCCACTGTGAAGCTGCAGTGCAACAACACCAAGTACCCGCTCGTCGTTAAAGGCACGACGGACAAGAACGGCTACTTCTTCATCTCAGCTCCCAAGACCATCACCACTTTCGGTGCACACAAATGCAAGGCCTTCCTTGTCTCCGCTCCCACCACCGCGTGTTCCAAGATCAGCGACTTCCACGGCGGTCTCACCGGCGCCGTTCTAAGGCCAACAAAGCCATTCGTGTCCGAGAAGAAGCTCCCCTTCCTTCTCTACACCGTCGGTCCTTTCGCCTTCGAGTCCAAATGCCCTCGTTGA

Protein Analysis

277

Amino Acids

29.57

Weight (kDa)

9.8

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 152 - 243 1.7e-23 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34700
fragaria_vesca FvH4_3g09040
malus_domestica MD05G1338000.v1.1 MD10G1293100.v1.1
prunus_persica Prupe.4G048800_v2.0.a1
pyrus_communis pycom05g28950 pycom10g24510 pycom14g04310
rosa_chinensis RchiOBHm_Chr5g0008631
rosa_laevigata RLG00000031588
rosa_multiflora Rmu_sc0001665.1_g000026
rosa_roxburghii Rroxscaffold_1G00067920
rosa_rugosa Rorug04G0437500
rosa_samantha Rh5AG066000 Rh5BG063400 Rh5CG073600 Rh5DG062800
rosa_wichuraiana Rw5G006290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 400
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 3 cut(s) 103, 530, 728
AccBSI CCGCTC 2 cut(s) 571, 677
AccI GTMKAC 2 cut(s) 468, 498
AccII CGCG 2 cut(s) 112, 689
AcoI YGGCCR 1 cut(s) 169
AcyI GRCGYC 3 cut(s) 462, 495, 729
AdeI CACNNNGTG 1 cut(s) 690
AfaI GTAC 2 cut(s) 488, 566
AfiI CCNNNNNNNGG 2 cut(s) 167, 738
AgeI ACCGGT 1 cut(s) 100
AgsI TTSAA 1 cut(s) 458
AluBI AGCT 7 cut(s) 17, 34, 92, 444, 544, 620, 772
AluI AGCT 7 cut(s) 17, 34, 92, 444, 544, 620, 772
Alw21I GWGCWC 1 cut(s) 649
Alw26I GTCTC 2 cut(s) 676, 725
Alw44I GTGCAC 1 cut(s) 645
Ama87I CYCGRG 1 cut(s) 361
AoxI GGCC 3 cut(s) 169, 660, 740
ApaLI GTGCAC 1 cut(s) 645
ApeKI GCWGC 1 cut(s) 544
AsiGI ACCGGT 1 cut(s) 100
Asp700I GAANNNNTTC 1 cut(s) 431
AspLEI GCGC 2 cut(s) 114, 731
AspS9I GGNCC 1 cut(s) 797
AsuC2I CCSGG 2 cut(s) 221, 299
AvaI CYCGRG 1 cut(s) 361
AvaII GGWCC 1 cut(s) 797
BaeGI GKGCMC 1 cut(s) 649
BanI GGYRCC 3 cut(s) 103, 530, 728
Bbv12I GWGCWC 1 cut(s) 649
BbvI GCAGC 1 cut(s) 531
BccI CCATC 2 cut(s) 530, 638
BceAI ACGGC 6 cut(s) 136, 184, 437, 616, 716, 730
BcnI CCSGG 2 cut(s) 221, 299
BcoDI GTCTC 2 cut(s) 676, 725
BfaI CTAG 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 545
BfoI RGCGCY 1 cut(s) 732
BfuAI ACCTGC 1 cut(s) 400
BglI GCCNNNNNGGC 1 cut(s) 120
BisI GCNGC 4 cut(s) 107, 110, 144, 545
BlsI GCNGC 4 cut(s) 108, 111, 145, 546
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 221, 299
Bme18I GGWCC 1 cut(s) 797
BmeT110I CYCGRG 1 cut(s) 361
BmgT120I GGNCC 1 cut(s) 797
BmiI GGNNCC 3 cut(s) 105, 532, 730
BmrFI CCNGG 2 cut(s) 221, 299
BmrI ACTGGG 1 cut(s) 404
BmuI ACTGGG 1 cut(s) 404
BplI GAGNNNNNCTC 2 cut(s) 355, 387
BpuMI CCSGG 2 cut(s) 221, 299
BsaHI GRCGYC 3 cut(s) 462, 495, 729
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 4 cut(s) 118, 219, 297, 712
BsaWI WCCGGW 1 cut(s) 100
Bsc4I CCNNNNNNNGG 2 cut(s) 167, 738
Bse118I RCCGGY 3 cut(s) 100, 527, 725
Bse1I ACTGG 1 cut(s) 410
BseDI CCNNGG 4 cut(s) 118, 219, 297, 712
BseLI CCNNNNNNNGG 2 cut(s) 167, 738
BseMII CTCAG 2 cut(s) 107, 630
BseNI ACTGG 1 cut(s) 410
BseRI GAGGAG 3 cut(s) 35, 38, 497
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 1 cut(s) 531
Bsh1236I CGCG 2 cut(s) 112, 689
BshFI GGCC 3 cut(s) 171, 662, 742
BshNI GGYRCC 3 cut(s) 103, 530, 728
BshTI ACCGGT 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 649
BsiHKCI CYCGRG 1 cut(s) 361
BsiSI CCGG 7 cut(s) 101, 221, 233, 299, 492, 528, 726
BslI CCNNNNNNNGG 2 cut(s) 167, 738
BsmAI GTCTC 2 cut(s) 676, 725
BsnI GGCC 3 cut(s) 171, 662, 742
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 1 cut(s) 361
Bsp1286I GDGCHC 1 cut(s) 649
Bsp143I GATC 1 cut(s) 699
BspANI GGCC 3 cut(s) 171, 662, 742
BspCNI CTCAG 2 cut(s) 106, 629
BspFNI CGCG 2 cut(s) 112, 689
BspLI GGNNCC 3 cut(s) 105, 532, 730
BspMAI CTGCAG 1 cut(s) 549
BspMI ACCTGC 1 cut(s) 400
BspT107I GGYRCC 3 cut(s) 103, 530, 728
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 2 cut(s) 571, 677
BsrFI RCCGGY 3 cut(s) 100, 527, 725
BsrI ACTGG 1 cut(s) 410
BssAI RCCGGY 3 cut(s) 100, 527, 725
BssECI CCNNGG 4 cut(s) 118, 219, 297, 712
BssMI GATC 1 cut(s) 699
BssNI GRCGYC 3 cut(s) 462, 495, 729
Bst4CI ACNGT 4 cut(s) 64, 214, 538, 793
BstACI GRCGYC 3 cut(s) 462, 495, 729
BstAPI GCANNNNNTGC 2 cut(s) 392, 480
BstDEI CTNAG 4 cut(s) 93, 423, 616, 737
BstDSI CCRYGG 2 cut(s) 118, 712
BstFNI CGCG 2 cut(s) 112, 689
BstH2I RGCGCY 1 cut(s) 732
BstHHI GCGC 2 cut(s) 114, 731
BstKTI GATC 1 cut(s) 702
BstMAI GTCTC 2 cut(s) 676, 725
BstMBI GATC 1 cut(s) 699
BstMWI GCNNNNNNNGC 6 cut(s) 89, 120, 149, 392, 480, 748
BstSCI CCNGG 2 cut(s) 219, 297
BstSFI CTRYAG 1 cut(s) 545
BstSLI GKGCMC 1 cut(s) 649
BstUI CGCG 2 cut(s) 112, 689
BstV1I GCAGC 1 cut(s) 531
BsuRI GGCC 3 cut(s) 171, 662, 742
BtgI CCRYGG 2 cut(s) 118, 712
BtgZI GCGATG 1 cut(s) 508
BtsI GCAGTG 1 cut(s) 554
BtsIMutI CAGTG 2 cut(s) 534, 554
BveI ACCTGC 1 cut(s) 400
CfoI GCGC 2 cut(s) 114, 731
Cfr10I RCCGGY 3 cut(s) 100, 527, 725
Cfr13I GGNCC 1 cut(s) 797
CseI GACGC 2 cut(s) 451, 484
Csp6I GTAC 2 cut(s) 487, 565
CspAI ACCGGT 1 cut(s) 100
CviQI GTAC 2 cut(s) 487, 565
DdeI CTNAG 4 cut(s) 93, 423, 616, 737
DinI GGCGCC 1 cut(s) 730
DpnI GATC 1 cut(s) 701
DpnII GATC 1 cut(s) 699
DraIII CACNNNGTG 1 cut(s) 690
EaeI YGGCCR 1 cut(s) 169
Eco147I AGGCCT 1 cut(s) 662
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 797
Eco88I CYCGRG 1 cut(s) 361
EgeI GGCGCC 1 cut(s) 730
EheI GGCGCC 1 cut(s) 730
FaiI YATR 1 cut(s) 129
FalI AAGNNNNNCTT 4 cut(s) 416, 448, 761, 793
FauI CCCGC 2 cut(s) 391, 576
FblI GTMKAC 2 cut(s) 468, 498
Fnu4HI GCNGC 4 cut(s) 107, 110, 144, 545
Fsp4HI GCNGC 4 cut(s) 107, 110, 144, 545
FspBI CTAG 1 cut(s) 20
GlaI GCGC 2 cut(s) 113, 730
GluI GCNGC 4 cut(s) 107, 110, 144, 545
HaeII RGCGCY 1 cut(s) 732
HaeIII GGCC 3 cut(s) 171, 662, 742
HapII CCGG 7 cut(s) 101, 221, 233, 299, 492, 528, 726
HgaI GACGC 2 cut(s) 451, 484
HhaI GCGC 2 cut(s) 114, 731
Hin1I GRCGYC 3 cut(s) 462, 495, 729
Hin6I GCGC 2 cut(s) 112, 729
HinP1I GCGC 2 cut(s) 112, 729
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HinfI GANTC 1 cut(s) 812
HpaII CCGG 7 cut(s) 101, 221, 233, 299, 492, 528, 726
Hpy166II GTNNAC 6 cut(s) 226, 379, 415, 469, 499, 647
Hpy188I TCNGA 1 cut(s) 763
Hpy8I GTNNAC 6 cut(s) 226, 379, 415, 469, 499, 647
Hpy99I CGWCG 8 cut(s) 218, 296, 452, 467, 500, 578, 592, 797
HpyAV CCTTC 7 cut(s) 67, 297, 442, 673, 787, 791, 817
HpyCH4III ACNGT 4 cut(s) 64, 214, 538, 793
HpyCH4V TGCA 8 cut(s) 11, 131, 474, 483, 547, 552, 647, 657
HpyF10VI GCNNNNNNNGC 6 cut(s) 89, 120, 149, 392, 480, 748
HpyF3I CTNAG 4 cut(s) 93, 423, 616, 737
Hsp92I GRCGYC 3 cut(s) 462, 495, 729
HspAI GCGC 2 cut(s) 112, 729
KasI GGCGCC 1 cut(s) 728
Kzo9I GATC 1 cut(s) 699
LmnI GCTCC 6 cut(s) 247, 328, 406, 625, 682, 777
Lsp1109I GCAGC 1 cut(s) 531
MaeI CTAG 1 cut(s) 20
MaeIII GTNAC 1 cut(s) 96
MalI GATC 1 cut(s) 701
MbiI CCGCTC 2 cut(s) 571, 677
MboI GATC 1 cut(s) 699
MboII GAAGA 2 cut(s) 601, 778
MhlI GDGCHC 1 cut(s) 649
Mly113I GGCGCC 1 cut(s) 729
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 1 cut(s) 233
MroXI GAANNNNTTC 1 cut(s) 431
MseI TTAA 3 cut(s) 330, 354, 579
MslI CAYNNNNRTG 1 cut(s) 652
MspI CCGG 7 cut(s) 101, 221, 233, 299, 492, 528, 726
MspR9I CCNGG 2 cut(s) 221, 299
MvnI CGCG 2 cut(s) 112, 689
MwoI GCNNNNNNNGC 6 cut(s) 89, 120, 149, 392, 480, 748
NarI GGCGCC 1 cut(s) 729
NciI CCSGG 2 cut(s) 221, 299
NdeII GATC 1 cut(s) 699
NlaIV GGNNCC 3 cut(s) 105, 532, 730
NmuCI GTSAC 1 cut(s) 96
PaqCI CACCTGC 1 cut(s) 400
PceI AGGCCT 1 cut(s) 662
PdmI GAANNNNTTC 1 cut(s) 431
PinAI ACCGGT 1 cut(s) 100
PkrI GCNGC 4 cut(s) 108, 111, 145, 546
PleI GAGTC 1 cut(s) 820
PluTI GGCGCC 1 cut(s) 732
PpsI GAGTC 1 cut(s) 820
PspN4I GGNNCC 3 cut(s) 105, 532, 730
PspPI GGNCC 1 cut(s) 797
PstI CTGCAG 1 cut(s) 549
RsaI GTAC 2 cut(s) 488, 566
RsaNI GTAC 2 cut(s) 487, 565
RseI CAYNNNNRTG 1 cut(s) 652
SalI GTCGAC 1 cut(s) 497
SaqAI TTAA 3 cut(s) 330, 354, 579
SatI GCNGC 4 cut(s) 107, 110, 144, 545
Sau3AI GATC 1 cut(s) 699
Sau96I GGNCC 1 cut(s) 797
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 1 cut(s) 821
ScrFI CCNGG 2 cut(s) 221, 299
SduI GDGCHC 1 cut(s) 649
SfcI CTRYAG 1 cut(s) 545
SfoI GGCGCC 1 cut(s) 730
SgrAI CRCCGGYG 3 cut(s) 100, 527, 725
SinI GGWCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 652
SseBI AGGCCT 1 cut(s) 662
SspDI GGCGCC 1 cut(s) 728
SspMI CTAG 1 cut(s) 20
StuI AGGCCT 1 cut(s) 662
StyD4I CCNGG 2 cut(s) 219, 297
TaaI ACNGT 4 cut(s) 64, 214, 538, 793
TaqI TCGA 3 cut(s) 87, 498, 810
TaqII GACCGA 1 cut(s) 785
TatI WGTACW 1 cut(s) 486
TauI GCSGC 3 cut(s) 109, 112, 146
Tru1I TTAA 3 cut(s) 330, 354, 579
Tru9I TTAA 3 cut(s) 330, 354, 579
TscAI CASTG 2 cut(s) 541, 554
TseFI GTSAC 1 cut(s) 96
TseI GCWGC 1 cut(s) 544
Tsp45I GTSAC 1 cut(s) 96
TspDTI ATGAA 1 cut(s) 601
TspGWI ACGGA 2 cut(s) 280, 605
TspRI CASTG 2 cut(s) 541, 554
VneI GTGCAC 1 cut(s) 645
VpaK11BI GGWCC 1 cut(s) 797
XmiI GTMKAC 2 cut(s) 468, 498
XmnI GAANNNNTTC 1 cut(s) 431
XspI CTAG 1 cut(s) 20
ZrmI AGTACT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.