MD05G1338000.v1.1

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0012982
Physical Location & Seq
Forward (+)
45941000 .. 45942250
1251 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1338000.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGGTTCTCCTGCTGTGTTTGTGCAGCTCTCAGTCCTCCTACTGAGCTGGTTCACTGTCTTCGGTGATGTAATTACCACTTTACCCGCCATTCCTCCACACCACAGCGTTCACTATTCCCATCCAGTAGCTTCCCCAACCCACCCACCGGCTTACCCACCCACTCACGGCCAGCACCACCGCCACCCCCACCCACACCCACCCACTGCCTCCCCGGTGCACCCACCAGCCCACTCTCCGTTTCACCCACCAAGCCCCCACCACCACCACGGCCACCCACCGGTTCACCCTCCTATGTACCCACCTAAGAAACCTTTCCCAAGGAGCTTTGTAGCGGTTCAAGGCGTCGTTTACTGCAAATCTTGCAACTACTCCGGCGTCGACACCCTTAACGGCGCCAAGCCAGTTCTTGGTGCTACAGTAAAGCTACAGTGCAACAACAGAAAGTTCCCATTGGTTGTGAAGGAAACCACAGATAAAAATGGCTACTTTTTTATCACGGCACCTAAGACCATCACCACCTTTGGAGCTCACAAGTGCAAGGTGTCACTCGTCTCCTCTCCTTCCGTCGCCTGCTCCAAGCCGTCCGATCTGCATGGTGGACTGAGCGGTGCTCTCATGAAGCCTGCGAAGCCATTTATGTCCCAGAAGCTCCCATTCCTTCTCTACAATGTCGGTCCGTTCGCCTTTGAGCCTACATGTCCTCGTTAA

Protein Analysis

237

Amino Acids

25.61

Weight (kDa)

9.67

Isoelectric Point (pI)

51.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 113 - 204 2.7e-24 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34700
fragaria_vesca FvH4_3g09040
malus_domestica MD05G1338000.v1.1 MD10G1293100.v1.1
prunus_persica Prupe.4G048800_v2.0.a1
pyrus_communis pycom05g28950 pycom10g24510 pycom14g04310
rosa_chinensis RchiOBHm_Chr5g0008631
rosa_laevigata RLG00000031588
rosa_multiflora Rmu_sc0001665.1_g000026
rosa_roxburghii Rroxscaffold_1G00067920
rosa_rugosa Rorug04G0437500
rosa_samantha Rh5AG066000 Rh5BG063400 Rh5CG073600 Rh5DG062800
rosa_wichuraiana Rw5G006290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 502
AccB7I CCANNNNNTGG 1 cut(s) 410
AccBSI CCGCTC 1 cut(s) 609
AccI GTMKAC 1 cut(s) 381
AciI CCGC 4 cut(s) 87, 181, 335, 609
AcoI YGGCCR 2 cut(s) 169, 271
AcyI GRCGYC 3 cut(s) 345, 378, 396
AfaI GTAC 1 cut(s) 299
AfiI CCNNNNNNNGG 4 cut(s) 148, 167, 280, 410
AflIII ACRYGT 1 cut(s) 698
AgeI ACCGGT 1 cut(s) 280
AgsI TTSAA 1 cut(s) 341
AluBI AGCT 7 cut(s) 28, 48, 131, 327, 427, 530, 652
AluI AGCT 7 cut(s) 28, 48, 131, 327, 427, 530, 652
Alw21I GWGCWC 3 cut(s) 222, 532, 616
Alw26I GTCTC 1 cut(s) 559
Alw44I GTGCAC 1 cut(s) 218
AoxI GGCC 2 cut(s) 169, 271
ApaLI GTGCAC 1 cut(s) 218
ApeKI GCWGC 1 cut(s) 25
AsiGI ACCGGT 1 cut(s) 280
Asp700I GAANNNNTTC 1 cut(s) 314
AspLEI GCGC 1 cut(s) 398
AspS9I GGNCC 1 cut(s) 677
AsuC2I CCSGG 1 cut(s) 215
AsuHPI GGTGA 4 cut(s) 77, 236, 278, 508
AvaII GGWCC 1 cut(s) 677
BaeGI GKGCMC 1 cut(s) 222
BanI GGYRCC 2 cut(s) 395, 502
BanII GRGCYC 1 cut(s) 532
BbsI GAAGAC 1 cut(s) 52
Bbv12I GWGCWC 3 cut(s) 222, 532, 616
BbvI GCAGC 1 cut(s) 37
BccI CCATC 2 cut(s) 129, 521
BceAI ACGGC 5 cut(s) 184, 286, 409, 516, 568
BcnI CCSGG 1 cut(s) 215
BcoDI GTCTC 1 cut(s) 559
BfmI CTRYAG 2 cut(s) 417, 428
BfoI RGCGCY 1 cut(s) 399
BisI GCNGC 1 cut(s) 26
BlsI GCNGC 1 cut(s) 27
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 1 cut(s) 677
BmgT120I GGNCC 1 cut(s) 677
BmiI GGNNCC 2 cut(s) 397, 504
BmrFI CCNGG 1 cut(s) 215
BpiI GAAGAC 1 cut(s) 52
BplI GAGNNNNNCTC 2 cut(s) 598, 630
BpuMI CCSGG 1 cut(s) 215
BsaHI GRCGYC 3 cut(s) 345, 378, 396
BsaJI CCNNGG 3 cut(s) 213, 268, 320
BsaWI WCCGGW 1 cut(s) 280
Bsc4I CCNNNNNNNGG 4 cut(s) 148, 167, 280, 410
Bse118I RCCGGY 2 cut(s) 148, 280
Bse1I ACTGG 2 cut(s) 125, 404
BseDI CCNNGG 3 cut(s) 213, 268, 320
BseGI GGATG 1 cut(s) 121
BseLI CCNNNNNNNGG 4 cut(s) 148, 167, 280, 410
BseMII CTCAG 3 cut(s) 35, 45, 596
BseNI ACTGG 2 cut(s) 125, 404
BseRI GAGGAG 1 cut(s) 547
BseSI GKGCMC 1 cut(s) 222
BseXI GCAGC 1 cut(s) 37
BsgI GTGCAG 1 cut(s) 44
BshFI GGCC 2 cut(s) 171, 273
BshNI GGYRCC 2 cut(s) 395, 502
BshTI ACCGGT 1 cut(s) 280
BsiHKAI GWGCWC 3 cut(s) 222, 532, 616
BsiSI CCGG 4 cut(s) 149, 215, 281, 375
BslFI GGGAC 1 cut(s) 628
BslI CCNNNNNNNGG 4 cut(s) 148, 167, 280, 410
BsmAI GTCTC 1 cut(s) 559
BsmBI CGTCTC 1 cut(s) 559
BsmFI GGGAC 1 cut(s) 628
BsnI GGCC 2 cut(s) 171, 273
Bsp1286I GDGCHC 3 cut(s) 222, 532, 616
Bsp143I GATC 1 cut(s) 589
BspACI CCGC 4 cut(s) 87, 181, 335, 609
BspANI GGCC 2 cut(s) 171, 273
BspCNI CTCAG 3 cut(s) 36, 44, 597
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 397, 504
BspT107I GGYRCC 2 cut(s) 395, 502
BsrBI CCGCTC 1 cut(s) 609
BsrFI RCCGGY 2 cut(s) 148, 280
BsrI ACTGG 2 cut(s) 125, 404
BssAI RCCGGY 2 cut(s) 148, 280
BssECI CCNNGG 3 cut(s) 213, 268, 320
BssMI GATC 1 cut(s) 589
BssNI GRCGYC 3 cut(s) 345, 378, 396
BssT1I CCWWGG 1 cut(s) 320
Bst4CI ACNGT 3 cut(s) 58, 421, 432
BstACI GRCGYC 3 cut(s) 345, 378, 396
BstAPI GCANNNNNTGC 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 173, 574, 627
BstDEI CTNAG 5 cut(s) 31, 44, 306, 507, 605
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 1 cut(s) 121
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 1 cut(s) 398
BstKTI GATC 1 cut(s) 592
BstMAI GTCTC 1 cut(s) 559
BstMBI GATC 1 cut(s) 589
BstMWI GCNNNNNNNGC 2 cut(s) 363, 631
BstNSI RCATGY 1 cut(s) 702
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 2 cut(s) 417, 428
BstSLI GKGCMC 1 cut(s) 222
BstV1I GCAGC 1 cut(s) 37
BstV2I GAAGAC 1 cut(s) 52
BsuRI GGCC 2 cut(s) 171, 273
BtgI CCRYGG 1 cut(s) 268
BtsCI GGATG 1 cut(s) 121
BtsI GCAGTG 1 cut(s) 204
BtsIMutI CAGTG 3 cut(s) 54, 204, 437
Cac8I GCNNGC 3 cut(s) 173, 574, 627
CciI TCATGA 1 cut(s) 618
CfoI GCGC 1 cut(s) 398
Cfr10I RCCGGY 2 cut(s) 148, 280
Cfr13I GGNCC 1 cut(s) 677
CpoI CGGWCCG 1 cut(s) 677
CseI GACGC 2 cut(s) 334, 367
Csp6I GTAC 1 cut(s) 298
CspAI ACCGGT 1 cut(s) 280
CspI CGGWCCG 1 cut(s) 677
CviAII CATG 3 cut(s) 596, 619, 699
CviQI GTAC 1 cut(s) 298
DdeI CTNAG 5 cut(s) 31, 44, 306, 507, 605
DinI GGCGCC 1 cut(s) 397
DpnI GATC 1 cut(s) 591
DpnII GATC 1 cut(s) 589
EaeI YGGCCR 2 cut(s) 169, 271
Ecl136II GAGCTC 1 cut(s) 530
Eco130I CCWWGG 1 cut(s) 320
Eco24I GRGCYC 1 cut(s) 532
Eco47I GGWCC 1 cut(s) 677
Eco53kI GAGCTC 1 cut(s) 530
EcoICRI GAGCTC 1 cut(s) 530
EcoT14I CCWWGG 1 cut(s) 320
EcoT38I GRGCYC 1 cut(s) 532
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 320
Esp3I CGTCTC 1 cut(s) 559
FaeI CATG 3 cut(s) 599, 622, 702
FaiI YATR 5 cut(s) 296, 597, 620, 641, 700
FaqI GGGAC 1 cut(s) 628
FatI CATG 3 cut(s) 595, 618, 698
FauI CCCGC 1 cut(s) 94
FblI GTMKAC 1 cut(s) 381
Fnu4HI GCNGC 1 cut(s) 26
FokI GGATG 1 cut(s) 108
FriOI GRGCYC 1 cut(s) 532
Fsp4HI GCNGC 1 cut(s) 26
GlaI GCGC 1 cut(s) 397
GluI GCNGC 1 cut(s) 26
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 2 cut(s) 171, 273
HapII CCGG 4 cut(s) 149, 215, 281, 375
HgaI GACGC 2 cut(s) 334, 367
HhaI GCGC 1 cut(s) 398
Hin1I GRCGYC 3 cut(s) 345, 378, 396
Hin1II CATG 3 cut(s) 599, 622, 702
Hin6I GCGC 1 cut(s) 396
HinP1I GCGC 1 cut(s) 396
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HpaII CCGG 4 cut(s) 149, 215, 281, 375
HphI GGTGA 4 cut(s) 77, 236, 278, 508
Hpy166II GTNNAC 7 cut(s) 54, 112, 220, 286, 352, 382, 602
Hpy188I TCNGA 1 cut(s) 589
Hpy188III TCNNGA 1 cut(s) 619
Hpy8I GTNNAC 7 cut(s) 54, 112, 220, 286, 352, 382, 602
Hpy99I CGWCG 3 cut(s) 350, 383, 572
HpyAV CCTTC 3 cut(s) 457, 573, 671
HpyCH4III ACNGT 3 cut(s) 58, 421, 432
HpyCH4V TGCA 7 cut(s) 25, 220, 357, 366, 435, 540, 595
HpyF10VI GCNNNNNNNGC 2 cut(s) 363, 631
HpyF3I CTNAG 5 cut(s) 31, 44, 306, 507, 605
Hsp92I GRCGYC 3 cut(s) 345, 378, 396
Hsp92II CATG 3 cut(s) 599, 622, 702
HspAI GCGC 1 cut(s) 396
KasI GGCGCC 1 cut(s) 395
Kzo9I GATC 1 cut(s) 589
LmnI GCTCC 4 cut(s) 324, 527, 581, 657
Lsp1109I GCAGC 1 cut(s) 37
MaeIII GTNAC 1 cut(s) 546
MalI GATC 1 cut(s) 591
MbiI CCGCTC 1 cut(s) 609
MboI GATC 1 cut(s) 589
MboII GAAGA 1 cut(s) 52
MhlI GDGCHC 3 cut(s) 222, 532, 616
MluCI AATT 1 cut(s) 72
Mly113I GGCGCC 1 cut(s) 396
MnlI CCTC 5 cut(s) 47, 105, 220, 300, 568
MroXI GAANNNNTTC 1 cut(s) 314
MseI TTAA 2 cut(s) 390, 709
MspI CCGG 4 cut(s) 149, 215, 281, 375
MspR9I CCNGG 1 cut(s) 215
MwoI GCNNNNNNNGC 2 cut(s) 363, 631
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 1 cut(s) 215
NdeII GATC 1 cut(s) 589
NlaIII CATG 3 cut(s) 599, 622, 702
NlaIV GGNNCC 2 cut(s) 397, 504
NmuCI GTSAC 1 cut(s) 546
NspI RCATGY 1 cut(s) 702
PagI TCATGA 1 cut(s) 618
PciI ACATGT 1 cut(s) 698
PdmI GAANNNNTTC 1 cut(s) 314
PflMI CCANNNNNTGG 1 cut(s) 410
PinAI ACCGGT 1 cut(s) 280
PkrI GCNGC 1 cut(s) 27
PluTI GGCGCC 1 cut(s) 399
PscI ACATGT 1 cut(s) 698
Psp124BI GAGCTC 1 cut(s) 532
PspN4I GGNNCC 2 cut(s) 397, 504
PspPI GGNCC 1 cut(s) 677
RsaI GTAC 1 cut(s) 299
RsaNI GTAC 1 cut(s) 298
Rsr2I CGGWCCG 1 cut(s) 677
RsrII CGGWCCG 1 cut(s) 677
SacI GAGCTC 1 cut(s) 532
SalI GTCGAC 1 cut(s) 380
SaqAI TTAA 2 cut(s) 390, 709
SatI GCNGC 1 cut(s) 26
Sau3AI GATC 1 cut(s) 589
Sau96I GGNCC 1 cut(s) 677
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 3 cut(s) 222, 532, 616
SfcI CTRYAG 2 cut(s) 417, 428
SfoI GGCGCC 1 cut(s) 397
SinI GGWCC 1 cut(s) 677
Sse9I AATT 1 cut(s) 72
SsiI CCGC 4 cut(s) 87, 181, 335, 609
SspDI GGCGCC 1 cut(s) 395
SstI GAGCTC 1 cut(s) 532
StyD4I CCNGG 1 cut(s) 213
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 3 cut(s) 58, 421, 432
TaqI TCGA 1 cut(s) 381
TaqII GACCGA 1 cut(s) 665
TasI AATT 1 cut(s) 72
Tru1I TTAA 2 cut(s) 390, 709
Tru9I TTAA 2 cut(s) 390, 709
TscAI CASTG 3 cut(s) 61, 211, 437
TseFI GTSAC 1 cut(s) 546
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 546
TspDTI ATGAA 1 cut(s) 635
TspGWI ACGGA 3 cut(s) 228, 556, 669
TspRI CASTG 3 cut(s) 61, 211, 437
Van91I CCANNNNNTGG 1 cut(s) 410
VneI GTGCAC 1 cut(s) 218
VpaK11BI GGWCC 1 cut(s) 677
XceI RCATGY 1 cut(s) 702
XmiI GTMKAC 1 cut(s) 381
XmnI GAANNNNTTC 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.