Rroxscaffold_1G00067920

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0012982
Physical Location & Seq
Forward (+)
89211565 .. 89212632
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00067920.1

Sequence Viewer

Length: 525 bp
ATGCCGGCTCTCTCTCCCTACCACCACGCTCCCGTTCAGCCCCCGAGCCATCCTCCCACTCACCACGCGCCACCGGCTCACTCCCCTGCCCCGGTCCACCCTCCTACCTACCCTCCCAAGAAGCCCTTCCCGCGTAGCTTCGTGGCCGTTCAAGGCGTCGTCTACTGCAAGTCGTGCAAGTACTCCGGCGTCGACACTCTCCTCAACGCCACCGCCATTGCCGGTGCTACGGTGAAGCTCCAGTGCAACAACACCAAGTACCCGCTTGTCGTGAAGGGCACGACTGACAAGCACGGCTACTTCTTCATCACAGCTCCCAAGACCGTCACAACCTACGGTGCCCACAAGTGCAAGGCCTCGCTAGTCTCCGCTCCGACCACCGCGTGTTCCAAGATCACCGACTTCCACGGCGGACTTACGGGCGCCGTTCTGAGGCCATCCAAGCCGTTCTTGTCGGAGAAGAAGCTCCCCTTCCTTCTGTACACCGTCGGTCCGTTCGCCTTCGAGTCCAAATGCCCCCATTGA

Protein Analysis

174

Amino Acids

18.66

Weight (kDa)

9.63

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pollen_Ole_e_1 PF01190 50 - 141 1.3e-24 Pollen protein Ole e 1 like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012982)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34700
fragaria_vesca FvH4_3g09040
malus_domestica MD05G1338000.v1.1 MD10G1293100.v1.1
prunus_persica Prupe.4G048800_v2.0.a1
pyrus_communis pycom05g28950 pycom10g24510 pycom14g04310
rosa_chinensis RchiOBHm_Chr5g0008631
rosa_laevigata RLG00000031588
rosa_multiflora Rmu_sc0001665.1_g000026
rosa_roxburghii Rroxscaffold_1G00067920
rosa_rugosa Rorug04G0437500
rosa_samantha Rh5AG066000 Rh5BG063400 Rh5CG073600 Rh5DG062800
rosa_wichuraiana Rw5G006290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 338, 422
AccBSI CCGCTC 1 cut(s) 371
AccI GTMKAC 2 cut(s) 162, 192
AccII CGCG 3 cut(s) 68, 133, 383
AciI CCGC 6 cut(s) 131, 213, 263, 369, 381, 411
AcoI YGGCCR 1 cut(s) 144
AcyI GRCGYC 3 cut(s) 156, 189, 423
AdeI CACNNNGTG 1 cut(s) 384
AfaI GTAC 3 cut(s) 182, 260, 482
AfiI CCNNNNNNNGG 2 cut(s) 91, 432
AgsI TTSAA 1 cut(s) 152
AjuI GAANNNNNNNTTGG 2 cut(s) 110, 142
AluBI AGCT 4 cut(s) 138, 238, 314, 466
AluI AGCT 4 cut(s) 138, 238, 314, 466
Alw26I GTCTC 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 43
AoxI GGCC 3 cut(s) 144, 354, 434
Asp700I GAANNNNTTC 1 cut(s) 125
AspLEI GCGC 2 cut(s) 70, 425
AspS9I GGNCC 2 cut(s) 94, 491
AsuC2I CCSGG 1 cut(s) 92
AsuHPI GGTGA 3 cut(s) 53, 244, 388
AvaI CYCGRG 1 cut(s) 43
AvaII GGWCC 2 cut(s) 94, 491
BaeGI GKGCMC 2 cut(s) 281, 343
BanI GGYRCC 2 cut(s) 338, 422
BccI CCATC 2 cut(s) 57, 445
BceAI ACGGC 5 cut(s) 131, 310, 410, 424, 430
BcnI CCSGG 1 cut(s) 92
BcoDI GTCTC 1 cut(s) 370
BfaI CTAG 1 cut(s) 362
BfoI RGCGCY 1 cut(s) 426
BmcAI AGTACT 1 cut(s) 182
Bme1390I CCNGG 1 cut(s) 92
Bme18I GGWCC 2 cut(s) 94, 491
BmeT110I CYCGRG 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 94, 491
BmiI GGNNCC 2 cut(s) 340, 424
BmrFI CCNGG 1 cut(s) 92
BplI GAGNNNNNCTC 2 cut(s) 37, 69
BpmI CTGGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 92
BsaHI GRCGYC 3 cut(s) 156, 189, 423
BsaJI CCNNGG 2 cut(s) 90, 406
BsaXI ACNNNNNCTCC 2 cut(s) 97, 127
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 432
Bse118I RCCGGY 3 cut(s) 4, 73, 221
Bse1I ACTGG 1 cut(s) 241
Bse3DI GCAATG 1 cut(s) 216
BseDI CCNNGG 2 cut(s) 90, 406
BseGI GGATG 2 cut(s) 49, 437
BseLI CCNNNNNNNGG 2 cut(s) 91, 432
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 422
BseNI ACTGG 1 cut(s) 241
BseRI GAGGAG 1 cut(s) 191
BseSI GKGCMC 2 cut(s) 281, 343
Bsh1236I CGCG 3 cut(s) 68, 133, 383
BshFI GGCC 3 cut(s) 146, 356, 436
BshNI GGYRCC 2 cut(s) 338, 422
BsiHKCI CYCGRG 1 cut(s) 43
BsiSI CCGG 5 cut(s) 5, 74, 92, 186, 222
BslI CCNNNNNNNGG 2 cut(s) 91, 432
BsmAI GTCTC 1 cut(s) 370
BsnI GGCC 3 cut(s) 146, 356, 436
BsoBI CYCGRG 1 cut(s) 43
Bsp1286I GDGCHC 2 cut(s) 281, 343
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 1 cut(s) 393
BspACI CCGC 6 cut(s) 131, 213, 263, 369, 381, 411
BspANI GGCC 3 cut(s) 146, 356, 436
BspCNI CTCAG 1 cut(s) 423
BspFNI CGCG 3 cut(s) 68, 133, 383
BspLI GGNNCC 2 cut(s) 340, 424
BspT107I GGYRCC 2 cut(s) 338, 422
BsrBI CCGCTC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 3 cut(s) 4, 73, 221
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 1 cut(s) 241
BssAI RCCGGY 3 cut(s) 4, 73, 221
BssECI CCNNGG 2 cut(s) 90, 406
BssMI GATC 1 cut(s) 393
BssNI GRCGYC 3 cut(s) 156, 189, 423
Bst4CI ACNGT 4 cut(s) 232, 325, 338, 487
BstACI GRCGYC 3 cut(s) 156, 189, 423
BstAPI GCANNNNNTGC 1 cut(s) 174
BstAUI TGTACA 1 cut(s) 480
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 1 cut(s) 431
BstDSI CCRYGG 1 cut(s) 406
BstF5I GGATG 2 cut(s) 49, 437
BstFNI CGCG 3 cut(s) 68, 133, 383
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 70, 425
BstKTI GATC 1 cut(s) 396
BstMAI GTCTC 1 cut(s) 370
BstMBI GATC 1 cut(s) 393
BstMWI GCNNNNNNNGC 4 cut(s) 74, 130, 174, 442
BstSCI CCNGG 1 cut(s) 90
BstSLI GKGCMC 2 cut(s) 281, 343
BstUI CGCG 3 cut(s) 68, 133, 383
BsuRI GGCC 3 cut(s) 146, 356, 436
BtgI CCRYGG 1 cut(s) 406
BtsCI GGATG 2 cut(s) 49, 437
BtsIMutI CAGTG 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 70, 425
Cfr10I RCCGGY 3 cut(s) 4, 73, 221
Cfr13I GGNCC 2 cut(s) 94, 491
CpoI CGGWCCG 1 cut(s) 491
CseI GACGC 2 cut(s) 145, 178
Csp6I GTAC 3 cut(s) 181, 259, 481
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CspI CGGWCCG 1 cut(s) 491
CviQI GTAC 3 cut(s) 181, 259, 481
DdeI CTNAG 1 cut(s) 431
DinI GGCGCC 1 cut(s) 424
DpnI GATC 1 cut(s) 395
DpnII GATC 1 cut(s) 393
DraIII CACNNNGTG 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 144
EciI GGCGGA 1 cut(s) 426
Eco147I AGGCCT 1 cut(s) 356
Eco47I GGWCC 2 cut(s) 94, 491
Eco88I CYCGRG 1 cut(s) 43
EgeI GGCGCC 1 cut(s) 424
EheI GGCGCC 1 cut(s) 424
FalI AAGNNNNNCTT 6 cut(s) 110, 142, 434, 466, 455, 487
FauI CCCGC 2 cut(s) 138, 270
FblI GTMKAC 2 cut(s) 162, 192
FokI GGATG 2 cut(s) 36, 424
FspBI CTAG 1 cut(s) 362
GlaI GCGC 2 cut(s) 69, 424
GsuI CTGGAG 1 cut(s) 224
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 3 cut(s) 146, 356, 436
HapII CCGG 5 cut(s) 5, 74, 92, 186, 222
HgaI GACGC 2 cut(s) 145, 178
HhaI GCGC 2 cut(s) 70, 425
Hin1I GRCGYC 3 cut(s) 156, 189, 423
Hin6I GCGC 2 cut(s) 68, 423
HinP1I GCGC 2 cut(s) 68, 423
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 1 cut(s) 506
HpaII CCGG 5 cut(s) 5, 74, 92, 186, 222
HphI GGTGA 3 cut(s) 53, 244, 388
Hpy166II GTNNAC 4 cut(s) 97, 163, 193, 483
Hpy188I TCNGA 3 cut(s) 375, 432, 457
Hpy188III TCNNGA 1 cut(s) 271
Hpy8I GTNNAC 4 cut(s) 97, 163, 193, 483
Hpy99I CGWCG 3 cut(s) 161, 194, 491
HpyAV CCTTC 5 cut(s) 136, 268, 481, 485, 511
HpyCH4III ACNGT 4 cut(s) 232, 325, 338, 487
HpyCH4V TGCA 4 cut(s) 168, 177, 246, 351
HpyF10VI GCNNNNNNNGC 4 cut(s) 74, 130, 174, 442
HpyF3I CTNAG 1 cut(s) 431
Hsp92I GRCGYC 3 cut(s) 156, 189, 423
HspAI GCGC 2 cut(s) 68, 423
KasI GGCGCC 1 cut(s) 422
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Kzo9I GATC 1 cut(s) 393
LmnI GCTCC 5 cut(s) 34, 243, 319, 376, 471
LpnPI CCDG 7 cut(s) 18, 87, 99, 105, 199, 235, 254
MaeI CTAG 1 cut(s) 362
MaeIII GTNAC 1 cut(s) 325
MalI GATC 1 cut(s) 395
MbiI CCGCTC 1 cut(s) 371
MboI GATC 1 cut(s) 393
MboII GAAGA 2 cut(s) 295, 472
MhlI GDGCHC 2 cut(s) 281, 343
Mly113I GGCGCC 1 cut(s) 423
MlyI GAGTC 1 cut(s) 515
MmeI TCCRAC 2 cut(s) 398, 435
MnlI CCTC 6 cut(s) 63, 111, 123, 212, 367, 426
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 1 cut(s) 125
MspI CCGG 5 cut(s) 5, 74, 92, 186, 222
MspR9I CCNGG 1 cut(s) 92
MvnI CGCG 3 cut(s) 68, 133, 383
MwoI GCNNNNNNNGC 4 cut(s) 74, 130, 174, 442
NaeI GCCGGC 1 cut(s) 6
NarI GGCGCC 1 cut(s) 423
NciI CCSGG 1 cut(s) 92
NdeII GATC 1 cut(s) 393
NgoMIV GCCGGC 1 cut(s) 4
NlaIV GGNNCC 2 cut(s) 340, 424
NmuCI GTSAC 1 cut(s) 325
PceI AGGCCT 1 cut(s) 356
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 1 cut(s) 125
PleI GAGTC 1 cut(s) 514
PluTI GGCGCC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 514
PspN4I GGNNCC 2 cut(s) 340, 424
PspPI GGNCC 2 cut(s) 94, 491
RsaI GTAC 3 cut(s) 182, 260, 482
RsaNI GTAC 3 cut(s) 181, 259, 481
Rsr2I CGGWCCG 1 cut(s) 491
RsrII CGGWCCG 1 cut(s) 491
SalI GTCGAC 1 cut(s) 191
Sau3AI GATC 1 cut(s) 393
Sau96I GGNCC 2 cut(s) 94, 491
ScaI AGTACT 1 cut(s) 182
SchI GAGTC 1 cut(s) 515
ScrFI CCNGG 1 cut(s) 92
SduI GDGCHC 2 cut(s) 281, 343
SetI ASST 6 cut(s) 110, 140, 240, 316, 335, 468
SfoI GGCGCC 1 cut(s) 424
SinI GGWCC 2 cut(s) 94, 491
SseBI AGGCCT 1 cut(s) 356
SsiI CCGC 6 cut(s) 131, 213, 263, 369, 381, 411
SspDI GGCGCC 1 cut(s) 422
SspMI CTAG 1 cut(s) 362
StuI AGGCCT 1 cut(s) 356
StyD4I CCNGG 1 cut(s) 90
TaaI ACNGT 4 cut(s) 232, 325, 338, 487
TaqI TCGA 2 cut(s) 192, 504
TaqII GACCGA 1 cut(s) 479
TatI WGTACW 2 cut(s) 180, 480
TscAI CASTG 1 cut(s) 248
TseFI GTSAC 1 cut(s) 325
Tsp45I GTSAC 1 cut(s) 325
TspDTI ATGAA 1 cut(s) 295
TspGWI ACGGA 1 cut(s) 483
TspRI CASTG 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 94, 491
XmiI GTMKAC 2 cut(s) 162, 192
XmnI GAANNNNTTC 1 cut(s) 125
XspI CTAG 1 cut(s) 362
ZrmI AGTACT 1 cut(s) 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.