FvH4_3g13290

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0011156
Physical Location & Seq
Forward (+)
7935837 .. 7942695
6859 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g13290.t1

Sequence Viewer

Length: 4137 bp
ATGGCATCATCAGTTGAAATGCCCTCTGCTCCATTCGGTTGCGTTTTGAGGGATCACAACCGCCGTGACCGGTGCAGAGAGACCAATGCCAGAGCCACCATGGGTGCTTTTCAGAAGAACCTCAAGAGTTTAGTGAGGGATCATCTCTGCATTGCAGTCTCTCCCAAGTCTGGCTCCAATGACGCCAACTCCGAGAACCAGAGTGTTAACAATGATGATTCTTGGGTTCGAAACGATGAGGCTTCTTGCTGTTCAAAGAAGGATGAGAACACCAACATAAATGGTGTGAGAGAAGATGCTTCGGGTTCGGTTATGACCCCAAGGCATGCTAGGGTGCTTGATCGATGGGCTTTCGCAAGACAAATGGTGTCGAAACCAGTTGAGAGGCAGAGGAGCGCAGAGGCAGAAGTGTTATGTAACAAGGATGAGAACAGCAATAGGAACAGTGACAGAGAAGATGACTCTGGTTCGGTCATGAGCCCGAGGCATGTCAGGGTTCTTGATCGATGGGATTTCGCAAGACAAATGGTGGCGAAACCGATTGAGAGGCAGAGGAGCGCAGACGCAGAGGTGTATTATGTGCCAAACTCGGCACCCTCTTCAGCAAGGACGTCAACTTCGAAGGAAGAGACTATAGCAAGAACAGATAGCTTGGCTGATCTCACCAAAAAAGGAGTGGGGGCTTCGTCTCTGGTTCAAATTTGGGAGCAGAGGCTCCACAAATCAGACACCCTCAAAACCAATTCCAGTGGAAATGAATTATCCGACACAAGTAGGTCTAGTTCTGGATTCAGCTCTACTGAGAATCAGAACGAGAATGAGACCAATGCTTCACCATCAAGACTTTCTGAAGCAGAAAAGGCAGAGTCCCCTGTTGAGAAAGCAGGGGATCCTGTTTTCTCTATTGAAGCAGGGGACTCTGATTTCCAGATGGAAAAAGCAAGGGAGTCTATTGATGAAACTTATGATGCCGGTGATTCCTTTGAAGATGCCAAAGGTATTGCAGATGATAGCCGGAATTTAGATGCTAAAGAAAAAGAAAAGGTTCGAGTTGTGGATATTATTAGAAGACTGACTGCACAGGGTCCATCGTCTTCCTTGAGTGAGGACAATAGTAATGATCATGATCAGCAGTATGGTAGCACGCAGAGTTCACCGTGTAGGGAATTGCTCCGTGAGCGTTCTGGGGTCGATCAGGCAGAACACAAAGGCTTATCAGAGCACAAAGGCTTGTCTCATGTTCTAAACTCGTCTCATGCTACAAGCTCACCACGCTTAAGAGGGAGGCAGGCATTTGCTGATTTGCTTATGCAGTTTGAGCGTGACAGGCATAAGGAACTTGACACGCTGGTGAACCAAAAGTCAGTATCGGAATTCCCACAGCGTGGTCGAACTCGAATTCAGTCATTACTTAGGCTTCGAATGCTTCAACGTGGTATGGCATTCGCTGGTTCAGATGTGCCACCGCTTCCACCAAAACCATCTCGAGTGAACAAACCTGCGCAAGGATCTAGCATCATGCATCTCAGGGAGAAATTTTGTACCAGTGTTGAGCAGGGCACCGAAGTGCAGGATGATGCGCCTAATCCAAATAGCCTTCAGAAGGACATGATAAAGAACACAAGTATTAATGTAGACAAGTTCTCCACCTTCAATAAGCCTACTGAAAGCACTCCTAATCGAGAAGTTAAGGTTATGGAGAAGCAGAAAAAATTCACAGTACTGCGTGTTAGTGAAGATGTTTGTGAAGCGACCCCCAGTTTGGACAAATCCTGCACCTCCAAGCCTGATGAAAGCACTCCTAATCGAGAAGTTAAGGTTATGGAACAGCAGAAAACATCCACAGTACTGCGTACTAGTGAAGATGTTTGTGAAGAAGCAACCCCAAGTTTAGACAAATCCTCCACCTCCAATAAGCCTAGTGAAAGCACTCCTAAGCGAGAAGTTAAGGTTATAGAGCAGCAGAAAGCATCCACAGCACTACGTGATAGCAAAGATGTTTGTGAAAAAGCGACCCCCAGTTTGGACAAATCCTGCACCTCCAATAAGCCTAGTGAAAACACTCGAAATCAAAAAGTTAAGGTTATAGAGCAGCAGAAAGCATCCACAGTACTACGTGCTAGTAAAGATGTTTGTGAAGAAGCGACCCCTAGTTTGGACAAATCCTGCACCTCCAATAAGCCAGGTGAAAGCGCTCCTAATCGAGAAGTCAAGGCTATAGAGCAGCAGAAAACATCCACAGTACTGCGTGCTAGTGAAGATGTTTGTGAAGAAAGGACCCCAAGTTTAGACAAATCCTCCACCTCAAATAAGCCTAATGAAAGAGTTGATGTTTTGGAGCAGCAGAAAACATCACCAGTATTGCGTGCTAGTGAAAATGTTTGTAAAGAAGCGACCCCAAGTTTAGAAGTTACCTTCCAAGAAACTAGCTCAGCTGCTCAAAGCTCCTTGCCAAATGTTAGTGAAGATCTTCGTCAAGTAGCCAGCCCAAGTCCAAAGGTCACCAGGCGAGAAACAAGCTTAGTTTCTACAAACCTTGATGTGCAAGGAACTGCCAATCCAACCACACCAATGTGTGATCCGAATGAGAATGAGCTATGGAATGGAAGAGGTTCAGTGGATAGAAACCTTGATTCACAAGAAACTGCAGATACAACAATATCCTCGAATGGTCATGATGGGGATGAAATATGGAAACCCACATGCTCAGAGGCTAGACACCTTGATTCACAACGAAGTGCAGATACAAGGACACCCTTGAGTGGTGGGGATGGGATTGGGAATGGGAATGGGAATGAAATATGTGAAGGAACAAGTTCAGAGGTTAGAAACCTTGACACACAAGAAACTGCAGATACAACCTCATCCTTGAACAGTTGGAATGAAAATGCATTATGCCATGGAACAAGTTTAGAGGCTGGAAACTTTGACTCACAAGAAAATATAGATACAACAACATCCATGGTCAACTGGGATGAGAATGATGATGATTTCCAGTACTATGGAGAAACAAATTATGACTGGATCAGTGAGATTTCTCGCCCACGGAGCTATTGGGAAGACATTAGGAAATCATGGTACCAAGAGATGCTCAACTCCAATTCAGAAAAAGGCGAGATACACCAACTCCTTGAAAGAAGAACAGTATCAAGCTTTCTTACTAGTGATTTCCGAGATAAGATAGACAGATTGATGGTATCTCGTGTAGAAAGACAAACATGTCACAATGTCAGTCAAGAAGAAGAAGATGACGACAGCAGCTATGATAGAATGAATCAAATGGTGTCTTTCTTACAACATCGCACAATCCCATCAGGCACCGAAGAAGAAGCAGAAGAAGTGCAAGAACATGTGCAGGTACAAGAACAAAACCAAGTCCAAGAACAAGTGATAGAATACGTGCAAGTCCAAGAACAAGTGCTAGACTACGTTCAAGTACAAGAACAGGTGCATGTACATCAAGACCAAGAAGACGAGGAGATGACAGAAGAGGAGGAGGAAGAAAATGATGACAATGACGATGATGATGAAGAATCAAGGAGCCTAATTAGTGGTCAATTTCTAGAAGCAAGTGATTATTTTGACCGTGCATCATTGCAGCCTTCACCATCTCACTTCACAACATGGAGTTACCAAGATAATGAAGTTGGGGATGATTCAGACCGAGCCACTTCTGCATCTCCTCGCCAACAGTCACCCTTTCGATATAGTCCACAACGTTCACCACCTCAATCTTACAACGCTAATCATCGGCAAGGCTCTCCCTCCCTCAGTCATCCTTTAATGGAATTGGAGCTGATATACGACATGAAAGGGCATATGATACAACTCTATCAAGAGATGTCTGAGCTAAGAAAAGCAATAAAGAGCTGCGTAGACATGCAAATGACAATGCAACAATCCATGAATCAGGATGTTCATTCAGGTCTAGTGGGGAAGAAAAGGAATTCTAATGGGTTAACAAAGAAAGGCAATTGCCGCATTTGCAATGAAGCAAAAATCGATTCACTATTGTACAGGTGTGGACACATGTGCACCTGTCTCAAGTGTGCTCATGATTTGCAATGGAACAGCGGAAAATGTCCGATTTGTCAAGCTCCGATTGCAGATGTGGTGAGGGCATACATGGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

1379

Amino Acids

154.39

Weight (kDa)

4.98

Isoelectric Point (pI)

55.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 1323 - 1370 1.3e-12 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 3219
AatII GACGTC 1 cut(s) 614
Acc16I TGCGCA 1 cut(s) 1503
Acc36I ACCTGC 2 cut(s) 1507, 3346
Acc65I GGTACC 1 cut(s) 3078
AccB1I GGYRCC 4 cut(s) 592, 1559, 3078, 3317
AccB7I CCANNNNNTGG 1 cut(s) 1385
AccI GTMKAC 2 cut(s) 1635, 3876
AciI CCGC 4 cut(s) 61, 1466, 3979, 4074
AclI AACGTT 1 cut(s) 3718
AclWI GGATC 7 cut(s) 60, 147, 884, 897, 1516, 2573, 3032
AcsI RAATTY 7 cut(s) 699, 1018, 1373, 1398, 1535, 1712, 3946
AcuI CTGAAG 3 cut(s) 585, 870, 1583
AcyI GRCGYC 2 cut(s) 183, 611
AdeI CACNNNGTG 2 cut(s) 1385, 1985
AfeI AGCGCT 1 cut(s) 2194
AfiI CCNNNNNNNGG 8 cut(s) 170, 1385, 1505, 1603, 1762, 2023, 2155, 2762
AflII CTTAAG 1 cut(s) 1276
AflIII ACRYGT 3 cut(s) 3218, 3349, 4029
AgeI ACCGGT 1 cut(s) 69
AhlI ACTAGT 2 cut(s) 1856, 3161
AjnI CCWGG 2 cut(s) 2182, 2504
AleI CACNNNNGTG 3 cut(s) 1349, 1565, 2572
AloI GAACNNNNNNTCC 2 cut(s) 2542, 2574
Alw21I GWGCWC 3 cut(s) 1224, 4037, 4054
Alw26I GTCTC 8 cut(s) 74, 163, 623, 693, 815, 1239, 1257, 4046
Alw44I GTGCAC 1 cut(s) 4033
AlwI GGATC 7 cut(s) 60, 147, 884, 897, 1516, 2573, 3032
Ama87I CYCGRG 2 cut(s) 481, 1485
Aor51HI AGCGCT 1 cut(s) 2194
ApaLI GTGCAC 1 cut(s) 4033
ApeKI GCWGC 8 cut(s) 1960, 2092, 2224, 2341, 2435, 3258, 3598, 3870
ApoI RAATTY 7 cut(s) 699, 1018, 1373, 1398, 1535, 1712, 3946
ArsI GACNNNNNNTTYG 8 cut(s) 601, 633, 1744, 1776, 2005, 2037, 2137, 2169
AseI ATTAAT 1 cut(s) 1629
AsiGI ACCGGT 1 cut(s) 69
Asp700I GAANNNNTTC 5 cut(s) 1044, 1712, 2469, 2611, 2815
Asp718I GGTACC 1 cut(s) 3078
AspLEI GCGC 5 cut(s) 398, 560, 1504, 1582, 2195
AspS9I GGNCC 2 cut(s) 1085, 2277
AsuII TTCGAA 3 cut(s) 229, 620, 1420
AvaI CYCGRG 2 cut(s) 481, 1485
AvaII GGWCC 2 cut(s) 1085, 2277
BaeGI GKGCMC 2 cut(s) 1562, 4037
BamHI GGATCC 1 cut(s) 889
BanI GGYRCC 4 cut(s) 592, 1559, 3078, 3317
BanII GRGCYC 1 cut(s) 482
BarI GAAGNNNNNNTAC 2 cut(s) 2728, 2760
BauI CACGAG 1 cut(s) 3201
BbsI GAAGAC 4 cut(s) 1075, 1086, 3066, 3477
Bbv12I GWGCWC 3 cut(s) 1224, 4037, 4054
BbvI GCAGC 8 cut(s) 1972, 2104, 2236, 2353, 2422, 3270, 3610, 3857
BceAI ACGGC 1 cut(s) 48
BciT130I CCWGG 2 cut(s) 2184, 2506
BclI TGATCA 2 cut(s) 1120, 1126
BcoDI GTCTC 8 cut(s) 74, 163, 623, 693, 815, 1239, 1257, 4046
BcuI ACTAGT 2 cut(s) 1856, 3161
BfmI CTRYAG 4 cut(s) 633, 2217, 2646, 2850
BfoI RGCGCY 1 cut(s) 2196
BfrI CTTAAG 1 cut(s) 1276
BfuAI ACCTGC 2 cut(s) 1507, 3346
BglII AGATCT 1 cut(s) 2467
BisI GCNGC 9 cut(s) 1961, 2093, 2225, 2342, 2436, 3259, 3599, 3871, 3979
BlpI GCTNAGC 1 cut(s) 2431
BlsI GCNGC 9 cut(s) 1962, 2094, 2226, 2343, 2437, 3260, 3600, 3872, 3980
BmcAI AGTACT 5 cut(s) 1722, 1848, 2112, 2244, 2999
Bme1390I CCNGG 2 cut(s) 2184, 2506
Bme18I GGWCC 2 cut(s) 1085, 2277
BmeT110I CYCGRG 2 cut(s) 481, 1485
BmgT120I GGNCC 2 cut(s) 1085, 2277
BmrFI CCNGG 2 cut(s) 2184, 2506
BmrI ACTGGG 3 cut(s) 1752, 2013, 2980
BmuI ACTGGG 3 cut(s) 1752, 2013, 2980
BpiI GAAGAC 4 cut(s) 1075, 1086, 3066, 3477
Bpu10I CCTNAGC 1 cut(s) 1935
Bpu1102I GCTNAGC 1 cut(s) 2431
Bpu14I TTCGAA 3 cut(s) 229, 620, 1420
BpuEI CTTGAG 4 cut(s) 107, 1120, 2779, 4028
Bsa29I ATCGAT 3 cut(s) 343, 505, 4002
BsaAI YACGTR 3 cut(s) 1985, 2117, 3400
BsaBI GATNNNNATC 1 cut(s) 1125
BsaHI GRCGYC 2 cut(s) 183, 611
BsaI GGTCTC 2 cut(s) 74, 815
BsaJI CCNNGG 6 cut(s) 99, 320, 482, 2899, 2961, 3044
BsaWI WCCGGW 1 cut(s) 69
Bsc4I CCNNNNNNNGG 8 cut(s) 170, 1385, 1505, 1603, 1762, 2023, 2155, 2762
Bse118I RCCGGY 2 cut(s) 69, 971
Bse1I ACTGG 9 cut(s) 377, 747, 1545, 1758, 2019, 2357, 2975, 2995, 3026
Bse3DI GCAATG 4 cut(s) 150, 3593, 3994, 4070
Bse8I GATNNNNATC 1 cut(s) 1125
BseBI CCWGG 2 cut(s) 2184, 2506
BseCI ATCGAT 3 cut(s) 343, 505, 4002
BseDI CCNNGG 6 cut(s) 99, 320, 482, 2899, 2961, 3044
BseJI GATNNNNATC 1 cut(s) 1125
BseLI CCNNNNNNNGG 8 cut(s) 170, 1385, 1505, 1603, 1762, 2023, 2155, 2762
BseMI GCAATG 4 cut(s) 150, 3593, 3994, 4070
BseMII CTCAG 6 cut(s) 792, 1540, 2445, 2721, 3784, 3837
BseNI ACTGG 9 cut(s) 377, 747, 1545, 1758, 2019, 2357, 2975, 2995, 3026
BseRI GAGGAG 6 cut(s) 406, 568, 3491, 3506, 3509, 3672
BseSI GKGCMC 2 cut(s) 1562, 4037
BseXI GCAGC 8 cut(s) 1972, 2104, 2236, 2353, 2422, 3270, 3610, 3857
BsgI GTGCAG 8 cut(s) 94, 1062, 1589, 1759, 2020, 2152, 2760, 3374
BshNI GGYRCC 4 cut(s) 592, 1559, 3078, 3317
BshTI ACCGGT 1 cut(s) 69
BshVI ATCGAT 3 cut(s) 343, 505, 4002
BsiHKAI GWGCWC 3 cut(s) 1224, 4037, 4054
BsiHKCI CYCGRG 2 cut(s) 481, 1485
BsiSI CCGG 3 cut(s) 70, 972, 1015
BslFI GGGAC 2 cut(s) 853, 929
BslI CCNNNNNNNGG 8 cut(s) 170, 1385, 1505, 1603, 1762, 2023, 2155, 2762
BsmAI GTCTC 8 cut(s) 74, 163, 623, 693, 815, 1239, 1257, 4046
BsmBI CGTCTC 2 cut(s) 693, 1257
BsmFI GGGAC 2 cut(s) 853, 929
BsmI GAATGC 2 cut(s) 1428, 1442
Bso31I GGTCTC 2 cut(s) 74, 815
BsoBI CYCGRG 2 cut(s) 481, 1485
Bsp119I TTCGAA 3 cut(s) 229, 620, 1420
Bsp1286I GDGCHC 5 cut(s) 482, 1224, 1562, 4037, 4054
Bsp1407I TGTACA 2 cut(s) 3454, 4014
Bsp1720I GCTNAGC 1 cut(s) 2431
Bsp19I CCATGG 3 cut(s) 99, 2899, 2961
BspACI CCGC 4 cut(s) 61, 1466, 3979, 4074
BspCNI CTCAG 6 cut(s) 793, 1539, 2444, 2720, 3783, 3838
BspDI ATCGAT 3 cut(s) 343, 505, 4002
BspHI TCATGA 4 cut(s) 474, 1123, 2674, 4054
BspMAI CTGCAG 2 cut(s) 2650, 2854
BspMI ACCTGC 2 cut(s) 1507, 3346
BspPI GGATC 7 cut(s) 60, 147, 884, 897, 1516, 2573, 3032
BspT104I TTCGAA 3 cut(s) 229, 620, 1420
BspT107I GGYRCC 4 cut(s) 592, 1559, 3078, 3317
BspTI CTTAAG 1 cut(s) 1276
BspTNI GGTCTC 2 cut(s) 74, 815
BsrDI GCAATG 4 cut(s) 150, 3593, 3994, 4070
BsrFI RCCGGY 2 cut(s) 69, 971
BsrGI TGTACA 2 cut(s) 3454, 4014
BsrI ACTGG 9 cut(s) 377, 747, 1545, 1758, 2019, 2357, 2975, 2995, 3026
BssAI RCCGGY 2 cut(s) 69, 971
BssECI CCNNGG 6 cut(s) 99, 320, 482, 2899, 2961, 3044
BssNI GRCGYC 2 cut(s) 183, 611
BssSI CACGAG 1 cut(s) 3201
BssT1I CCWWGG 4 cut(s) 99, 320, 2899, 2961
Bst2BI CACGAG 1 cut(s) 3201
Bst2UI CCWGG 2 cut(s) 2184, 2506
Bst6I CTCTTC 4 cut(s) 604, 621, 2602, 3483
BstACI GRCGYC 2 cut(s) 183, 611
BstAFI CTTAAG 1 cut(s) 1276
BstAUI TGTACA 2 cut(s) 3454, 4014
BstBAI YACGTR 3 cut(s) 1985, 2117, 3400
BstBI TTCGAA 3 cut(s) 229, 620, 1420
BstC8I GCNNGC 6 cut(s) 327, 1145, 1290, 2250, 2367, 2485
BstDSI CCRYGG 4 cut(s) 99, 2899, 2961, 3044
BstEII GGTNACC 1 cut(s) 2500
BstENI CCTNNNNNAGG 2 cut(s) 1503, 1601
BstH2I RGCGCY 1 cut(s) 2196
BstHHI GCGC 5 cut(s) 398, 560, 1504, 1582, 2195
BstMAI GTCTC 8 cut(s) 74, 163, 623, 693, 815, 1239, 1257, 4046
BstNI CCWGG 2 cut(s) 2184, 2506
BstNSI RCATGY 8 cut(s) 329, 491, 2706, 3222, 3353, 3455, 3883, 4033
BstPI GGTNACC 1 cut(s) 2500
BstSCI CCNGG 2 cut(s) 2182, 2504
BstSFI CTRYAG 4 cut(s) 633, 2217, 2646, 2850
BstSLI GKGCMC 2 cut(s) 1562, 4037
BstV1I GCAGC 8 cut(s) 1972, 2104, 2236, 2353, 2422, 3270, 3610, 3857
BstV2I GAAGAC 4 cut(s) 1075, 1086, 3066, 3477
BstX2I RGATCY 3 cut(s) 889, 1508, 2467
BstXI CCANNNNNNTGG 1 cut(s) 3002
BstYI RGATCY 3 cut(s) 889, 1508, 2467
Bsu15I ATCGAT 3 cut(s) 343, 505, 4002
BsuTUI ATCGAT 3 cut(s) 343, 505, 4002
BtgI CCRYGG 4 cut(s) 99, 2899, 2961, 3044
BtgZI GCGATG 1 cut(s) 3284
BtsIMutI CAGTG 5 cut(s) 451, 754, 1552, 2622, 3034
BveI ACCTGC 2 cut(s) 1507, 3346
Cac8I GCNNGC 6 cut(s) 327, 1145, 1290, 2250, 2367, 2485
CciI TCATGA 4 cut(s) 474, 1123, 2674, 4054
CfoI GCGC 5 cut(s) 398, 560, 1504, 1582, 2195
Cfr10I RCCGGY 2 cut(s) 69, 971
Cfr13I GGNCC 2 cut(s) 1085, 2277
ClaI ATCGAT 3 cut(s) 343, 505, 4002
CseI GACGC 2 cut(s) 191, 572
CspAI ACCGGT 1 cut(s) 69
CspCI CAANNNNNGTGG 2 cut(s) 730, 765
DraIII CACNNNGTG 2 cut(s) 1385, 1985
DrdI GACNNNNNNGTC 1 cut(s) 3219
DseDI GACNNNNNNGTC 1 cut(s) 3219
Eam1104I CTCTTC 4 cut(s) 604, 621, 2602, 3483
EarI CTCTTC 4 cut(s) 604, 621, 2602, 3483
Eco130I CCWWGG 4 cut(s) 99, 320, 2899, 2961
Eco24I GRGCYC 1 cut(s) 482
Eco31I GGTCTC 2 cut(s) 74, 815
Eco47I GGWCC 2 cut(s) 1085, 2277
Eco47III AGCGCT 1 cut(s) 2194
Eco57I CTGAAG 3 cut(s) 585, 870, 1583
Eco88I CYCGRG 2 cut(s) 481, 1485
Eco91I GGTNACC 1 cut(s) 2500
EcoNI CCTNNNNNAGG 2 cut(s) 1503, 1601
EcoO109I RGGNCCY 1 cut(s) 2277
EcoO65I GGTNACC 1 cut(s) 2500
EcoRI GAATTC 3 cut(s) 1373, 1398, 3946
EcoRII CCWGG 2 cut(s) 2182, 2504
EcoT14I CCWWGG 4 cut(s) 99, 320, 2899, 2961
EcoT22I ATGCAT 2 cut(s) 1524, 2893
EcoT38I GRGCYC 1 cut(s) 482
ErhI CCWWGG 4 cut(s) 99, 320, 2899, 2961
Esp3I CGTCTC 2 cut(s) 693, 1257
FaqI GGGAC 2 cut(s) 853, 929
FauNDI CATATG 1 cut(s) 3819
FbaI TGATCA 2 cut(s) 1120, 1126
FblI GTMKAC 2 cut(s) 1635, 3876
Fnu4HI GCNGC 9 cut(s) 1961, 2093, 2225, 2342, 2436, 3259, 3599, 3871, 3979
FriOI GRGCYC 1 cut(s) 482
Fsp4HI GCNGC 9 cut(s) 1961, 2093, 2225, 2342, 2436, 3259, 3599, 3871, 3979
FspI TGCGCA 1 cut(s) 1503
GlaI GCGC 5 cut(s) 397, 559, 1503, 1581, 2194
GluI GCNGC 9 cut(s) 1961, 2093, 2225, 2342, 2436, 3259, 3599, 3871, 3979
HaeII RGCGCY 1 cut(s) 2196
HapII CCGG 3 cut(s) 70, 972, 1015
HgaI GACGC 2 cut(s) 191, 572
HhaI GCGC 5 cut(s) 398, 560, 1504, 1582, 2195
Hin1I GRCGYC 2 cut(s) 183, 611
Hin6I GCGC 5 cut(s) 396, 558, 1502, 1580, 2193
HinP1I GCGC 5 cut(s) 396, 558, 1502, 1580, 2193
HincII GTYRAC 4 cut(s) 208, 615, 2968, 3960
HindII GTYRAC 4 cut(s) 208, 615, 2968, 3960
HindIII AAGCTT 2 cut(s) 2518, 3151
HpaI GTTAAC 2 cut(s) 208, 3960
HpaII CCGG 3 cut(s) 70, 972, 1015
HpyAV CCTTC 8 cut(s) 253, 616, 1597, 1607, 1660, 2425, 2801, 3612
HpyCH4IV ACGT 7 cut(s) 611, 1432, 1984, 2116, 3399, 3429, 3718
HpySE526I ACGT 7 cut(s) 611, 1432, 1984, 2116, 3399, 3429, 3718
Hsp92I GRCGYC 2 cut(s) 183, 611
HspAI GCGC 5 cut(s) 396, 558, 1502, 1580, 2193
KpnI GGTACC 1 cut(s) 3082
Ksp22I TGATCA 2 cut(s) 1120, 1126
KspAI GTTAAC 2 cut(s) 208, 3960
Lsp1109I GCAGC 8 cut(s) 1972, 2104, 2236, 2353, 2422, 3270, 3610, 3857
MaeII ACGT 7 cut(s) 611, 1432, 1984, 2116, 3399, 3429, 3718
MaeIII GTNAC 9 cut(s) 65, 416, 446, 1322, 2410, 2500, 3221, 3629, 3693
MfeI CAATTG 1 cut(s) 3973
MflI RGATCY 3 cut(s) 889, 1508, 2467
MhlI GDGCHC 5 cut(s) 482, 1224, 1562, 4037, 4054
MlyI GAGTC 5 cut(s) 455, 875, 911, 956, 2924
MmeI TCCRAC 3 cut(s) 789, 2585, 2858
Mph1103I ATGCAT 2 cut(s) 1524, 2893
MroXI GAANNNNTTC 5 cut(s) 1044, 1712, 2469, 2611, 2815
MslI CAYNNNNRTG 5 cut(s) 1349, 1565, 2570, 2572, 3884
MspA1I CMGCKG 2 cut(s) 2435, 4074
MspCI CTTAAG 1 cut(s) 1276
MspI CCGG 3 cut(s) 70, 972, 1015
MspR9I CCNGG 2 cut(s) 2184, 2506
MunI CAATTG 1 cut(s) 3973
Mva1269I GAATGC 2 cut(s) 1428, 1442
MvaI CCWGG 2 cut(s) 2184, 2506
NcoI CCATGG 3 cut(s) 99, 2899, 2961
NdeI CATATG 1 cut(s) 3819
NmeAIII GCCGAG 1 cut(s) 569
NmuCI GTSAC 6 cut(s) 65, 446, 1322, 2500, 3221, 3693
NsbI TGCGCA 1 cut(s) 1503
NsiI ATGCAT 2 cut(s) 1524, 2893
NspI RCATGY 8 cut(s) 329, 491, 2706, 3222, 3353, 3455, 3883, 4033
NspV TTCGAA 3 cut(s) 229, 620, 1420
OliI CACNNNNGTG 3 cut(s) 1349, 1565, 2572
PaeI GCATGC 1 cut(s) 329
PaeR7I CTCGAG 1 cut(s) 1485
PagI TCATGA 4 cut(s) 474, 1123, 2674, 4054
PciI ACATGT 3 cut(s) 3218, 3349, 4029
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 617
PctI GAATGC 2 cut(s) 1428, 1442
PdmI GAANNNNTTC 5 cut(s) 1044, 1712, 2469, 2611, 2815
PflMI CCANNNNNTGG 1 cut(s) 1385
PinAI ACCGGT 1 cut(s) 69
PkrI GCNGC 9 cut(s) 1962, 2094, 2226, 2343, 2437, 3260, 3600, 3872, 3980
PleI GAGTC 5 cut(s) 455, 874, 911, 955, 2924
PpsI GAGTC 5 cut(s) 455, 874, 911, 955, 2924
Ppu21I YACGTR 3 cut(s) 1985, 2117, 3400
PpuMI RGGWCCY 1 cut(s) 2277
PscI ACATGT 3 cut(s) 3218, 3349, 4029
PshBI ATTAAT 1 cut(s) 1629
Psp1406I AACGTT 1 cut(s) 3718
Psp5II RGGWCCY 1 cut(s) 2277
Psp6I CCWGG 2 cut(s) 2182, 2504
PspEI GGTNACC 1 cut(s) 2500
PspGI CCWGG 2 cut(s) 2182, 2504
PspPI GGNCC 2 cut(s) 1085, 2277
PspPPI RGGWCCY 1 cut(s) 2277
PsrI GAACNNNNNNTAC 2 cut(s) 766, 798
PstI CTGCAG 2 cut(s) 2650, 2854
PsuI RGATCY 3 cut(s) 889, 1508, 2467
PvuII CAGCTG 1 cut(s) 2435
RseI CAYNNNNRTG 5 cut(s) 1349, 1565, 2570, 2572, 3884
SatI GCNGC 9 cut(s) 1961, 2093, 2225, 2342, 2436, 3259, 3599, 3871, 3979
Sau96I GGNCC 2 cut(s) 1085, 2277
ScaI AGTACT 5 cut(s) 1722, 1848, 2112, 2244, 2999
SchI GAGTC 5 cut(s) 455, 875, 911, 956, 2924
ScrFI CCNGG 2 cut(s) 2184, 2506
SduI GDGCHC 5 cut(s) 482, 1224, 1562, 4037, 4054
SfcI CTRYAG 4 cut(s) 633, 2217, 2646, 2850
Sfr274I CTCGAG 1 cut(s) 1485
SfuI TTCGAA 3 cut(s) 229, 620, 1420
SinI GGWCC 2 cut(s) 1085, 2277
SlaI CTCGAG 1 cut(s) 1485
SmiMI CAYNNNNRTG 5 cut(s) 1349, 1565, 2570, 2572, 3884
SmlI CTYRAG 6 cut(s) 122, 1099, 1276, 1485, 2758, 4043
SmoI CTYRAG 6 cut(s) 122, 1099, 1276, 1485, 2758, 4043
SpeI ACTAGT 2 cut(s) 1856, 3161
SphI GCATGC 1 cut(s) 329
SsiI CCGC 4 cut(s) 61, 1466, 3979, 4074
StyD4I CCNGG 2 cut(s) 2182, 2504
StyI CCWWGG 4 cut(s) 99, 320, 2899, 2961
TaiI ACGT 7 cut(s) 614, 1435, 1987, 2119, 3402, 3432, 3721
TaqII GACCGA 2 cut(s) 460, 3678
TatI WGTACW 8 cut(s) 1720, 1846, 2110, 2242, 2997, 3436, 3454, 4014
TauI GCSGC 1 cut(s) 3981
TscAI CASTG 5 cut(s) 451, 754, 1552, 2622, 3034
TseFI GTSAC 6 cut(s) 65, 446, 1322, 2500, 3221, 3693
TseI GCWGC 8 cut(s) 1960, 2092, 2224, 2341, 2435, 3258, 3598, 3870
Tsp45I GTSAC 6 cut(s) 65, 446, 1322, 2500, 3221, 3693
TspGWI ACGGA 2 cut(s) 1163, 3061
TspRI CASTG 5 cut(s) 451, 754, 1552, 2622, 3034
Van91I CCANNNNNTGG 1 cut(s) 1385
Vha464I CTTAAG 1 cut(s) 1276
VneI GTGCAC 1 cut(s) 4033
VpaK11BI GGWCC 2 cut(s) 1085, 2277
VspI ATTAAT 1 cut(s) 1629
XagI CCTNNNNNAGG 2 cut(s) 1503, 1601
XapI RAATTY 7 cut(s) 699, 1018, 1373, 1398, 1535, 1712, 3946
XbaI TCTAGA 1 cut(s) 3562
XceI RCATGY 8 cut(s) 329, 491, 2706, 3222, 3353, 3455, 3883, 4033
XcmI CCANNNNNNNNNTGG 3 cut(s) 97, 673, 3051
XhoI CTCGAG 1 cut(s) 1485
XmiI GTMKAC 2 cut(s) 1635, 3876
XmnI GAANNNNTTC 5 cut(s) 1044, 1712, 2469, 2611, 2815
ZraI GACGTC 1 cut(s) 612
ZrmI AGTACT 5 cut(s) 1722, 1848, 2112, 2244, 2999
Zsp2I ATGCAT 2 cut(s) 1524, 2893
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.