MD10G1225200.v1.1

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0011156
Physical Location & Seq
Reverse (-)
32264733 .. 32269649
4917 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1225200.v1.1.491

Sequence Viewer

Length: 3342 bp
ATGGCATCATCACAAGTTGAAATGGCGTCTTCTGCGCCTTTCGGGTGCGTGTTGAGGGATCACAACCGGCGAGACAGGTGCCGAGAGAGAAATGCCAGGGCCACGCAGGCCGCTTTTCAGAAGAATTTGAATAGCTTGGTGAGGAATCACCTCACCACCTGCATTTCTGTCTCTTCAATATCTGCCTCCAGTGACAAGGAAAGCAACAACTCCAAAGACCGAGACATTGAAAATGTCGAAAGAGATGGTGGTTGTTCGACAATGAGCCCGAGGCAGTCTCGGGTTCTTGATCGACAGGCTGCGAGACGAGGAGGGAAAGAAGGAATGGCAATGGTTGAGAGGCAGAGCCAAGAGGCAGAGCTCATGTCAGTGCCTAACTCCGAACCTTCATCATCGAGGACATCGACATCTTTGAAGGAGACTCTTTTCAGAACGGATAGCTTGGCGAGAATTTACAATCTGGGAGTTGGAGCTTCCTCGCTGGTTCAAATATGGGAGAAGAGGCTGAACCAATCAAACAGTTTCAAGATGAGTTCCAATGGCAGCGCAGAGACAAGCAGGTCTAACTCTGGATTCAGCTGCAATGAGAATGAGAACATATTCAATGCTTCCAATGAATCCAGTGCTTCCAATGAGTCCACTGGTTCAAATGCACCAGTTTTCGAGAAACAATCATCGAGAGGGTCTGAAGCAGGGGACTCGGTTTGTGTTGATGAGAGGTATGACACCGGCCCGGTTGCTGAGGATTCATTTGAGGACACACATTCTGAGGAAAGCACCACAAGTGATGCCCTGAGTAATTTAGATGCAAGGGAAAGAGAAAGAGAAAGGGAGAAGGTTCGTGTTGCGGATATCATTAAAAGGTTGAAAATGACAGCACAGTGTCCATTATCTTCTAGTGATGACAATGATCAGCAGTCTTGTACCAATGCATCTCCGTCTAGAGAGCGCGATCATGAGAGGCTTTTCGGGTCTGATCAGACAGAGCAGCTGCACAAAGGGTTCTCTCAGGTTATAAGTTCACCGAGGATGATCAGAGGCAGGCAGGCTTTTGCTGATTTGCTTATGCACCTGGAGCGCGACCGACATAGGGAGCTTGACGCGCTTGTAGACCGCAGAGCAGTTTCAAGATTTTCACACAAAGGGCGAATTCAGTCAATGCTTCGGCTTAAACTGCTACAACGCCGCATGGCAGTGGCAGTAGGTCAAGATGTACCGCGCCGGAAACCAGCAACATCTAAAGTGAACAAACTTCCACAAGGGTCTGCCATCATGCATCTCAGGGAGAGATTTAGCACTGGTGTGGAGCAGGCCTCAACAGATCAGAGTGATGCAGCTATTCCAAAAAGCCCTCAAAAGGAGGTTGTAAAGAACACCAGTGTACTATCAAAAAGCTCCTCCACTCCCAATAAGCCTAGCAAAAACACTCTTAATCGTGACGTTGATAGAGTTGAGCAGAAGAATGCAACCGGGATGCAGACCTCATTGCCGCATGCTAGCGAAGATCTTCGTGAAGAAGTAGGCATGAGTTCAAAAGTCACCTGTCAAGAAACAAGTTCAGTGGCTACAAACACCTCAACGCAAGCTAGTAACGGTCTTCATGAGGAAGGTAGCCCAAGCTCAAAAGTCACCTCGGAAGGAACAAGTTCAGTGGCTACAAACAATTTTCCAAATGCTAGTGAAGATCTTCGTGATGAAGCCAGCCCGAGTTTGAAAGTCGCCTATCAAGGATCGAGTCCAATGGCTAGGAACACCACAACTCATGCTAGCGAAGGTCTTCATGAAGAAGCCAACCCGAGTTTAAAAGTCACCTTGCAGAGAACAAGTCTAATGGCTAGGAGTACCTCCCCTCATGGTAGTGAAGATCTTTGTGAAGAAGCGAGCCCGAGTTCAGAAGTCACCTTGCAAGGAACAAGTCCAATGGCTAGAAACACCTCACCTCATGTTAGTGAAGACCTTCACGAAGAAGTCAGCCCGCGCTCAGAAGTCACTTTGCAAGGAACAAGTTCAGAGGCCAGAAACAACTTACCTCATGCTAGTGAACATCCTCATGAAGAAGCTAGCCCTAGTTCAGAAATTATCTGGCAAGGAACAAGTTCAGAGGCCAGAAACAACTTGCCTCATGTTAGTGAAGATCTTCATGAAGAAGTTAGCCTGAGTTCAGAAGTTATCTGGCAAGGAACAAGTTCGAAGGCTAGAAACCTTGATGCGCAAGAAAATGCAGATGCAACCACATCCTTGGTTGGTTGGGGTGAGAATGAGATAGAAGAACAACTAGAGGATGATTACCAGTACTATGGAGATTATACTTATGACTGGATCAATGAGATTTCTCGCCCACGTAGCTACTGGGAAAACCTGAGAAAGGCATGGTACCAAGAGATGCTGAACTCCAACTCAGGCAAAGGGGAGATACGCCAACTTATCGAAAGAAGAACAGTATCAAATTGTCTTGCAAGTGATTTCCGAGACAGAATAGACAGACTGATGGAATCTCGCTTAGAAATACAAGCGCAACCAGATGGCAGTCAAGAAGGAGAAGACGATGATATCAGCCACGATAGGATGAATCAATTGATGACTTTCTTACAAAAGCATCACCAAAAACAACAACAACAATTGCTGCCAGCATGCAGTCAAGAAGAAAAACAACAAGTGCAAGAACAAGAACACCAAGAAGAAGATCAGAACATGACAGAAGAAGATGATGATGACAATGATGATGAAGGAGGAGAAGAAAGAAGTCTAATTAGCGCTCAATATCAAGAAGCAAGTGATGATTTTTACCAATGCACATCATTGCAGCCATCGCCATCTCACATGACAGCATGGAGCTATCAAGATATAGAAGTGGGGGATGATGCTAATCGAGCTGCTTCTACATCTCCACCCCGACATTTGCCATCTCATTCTTACTATTCAAATAGTCACCAAGGTTCACCATCACCACCTCCATCATACCATCAAAATCACCATCACTTCTCTTCCCCTGAAATGGAATTCATATATGACATGAGAGGGCAGATGGAGCAACTCTATCGTGAGATGTCGGAGCTACGAAAAGTAATTAAGAGCTGTGTAGACATGCAAATGGTAATGCAACAATCCATGAAGCAGGAAGTTCATTCAGGTCAAGAGGAGAGGAAAAGGTCTGCAAATGGATTACCAAAGAAAGGAAATTGTTGCATTTGCCATGAAGTGAAAGTCGACTCACTTTTGTACAGGTGTGGACACATGTGCACCTGTCTCAAGTGTGCTCATGAGTTGCAATGGAATAGTGGAAAATGTCCAATCTGTCGAGCTCCAATTGTGGATGTCGTGAAGGCACAGATGGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

1114

Amino Acids

124.47

Weight (kDa)

5.33

Isoelectric Point (pI)

64.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 1058 - 1105 1e-11 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1016
AarI CACCTGC 1 cut(s) 167
AasI GACNNNNNNGTC 1 cut(s) 559
Acc16I TGCGCA 1 cut(s) 2211
Acc36I ACCTGC 2 cut(s) 167, 549
Acc65I GGTACC 1 cut(s) 2373
AccB1I GGYRCC 2 cut(s) 78, 2373
AccI GTMKAC 3 cut(s) 1110, 3081, 3207
AccII CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
AciI CCGC 7 cut(s) 111, 848, 1114, 1186, 1217, 1490, 1976
AclWI GGATC 3 cut(s) 66, 1738, 2327
AcsI RAATTY 4 cut(s) 124, 450, 1149, 2999
AcuI CTGAAG 1 cut(s) 708
AcyI GRCGYC 1 cut(s) 26
AfaI GTAC 7 cut(s) 925, 1215, 1383, 1843, 2294, 2375, 3221
AfeI AGCGCT 1 cut(s) 2755
AfiI CCNNNNNNNGG 5 cut(s) 1090, 1357, 2365, 2995, 3173
AflIII ACRYGT 1 cut(s) 3234
AjnI CCWGG 2 cut(s) 95, 1071
AleI CACNNNNGTG 1 cut(s) 1301
Alw21I GWGCWC 4 cut(s) 363, 3242, 3259, 3304
Alw26I GTCTC 9 cut(s) 66, 175, 216, 282, 298, 413, 545, 2463, 3251
Alw44I GTGCAC 1 cut(s) 3238
AlwI GGATC 3 cut(s) 66, 1738, 2327
Ama87I CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
Aor51HI AGCGCT 1 cut(s) 2755
AoxI GGCC 6 cut(s) 99, 108, 730, 1311, 2013, 2103
ApaLI GTGCAC 1 cut(s) 3238
ApeKI GCWGC 9 cut(s) 299, 543, 579, 988, 991, 1334, 2623, 2803, 2873
ApoI RAATTY 4 cut(s) 124, 450, 1149, 2999
Asp718I GGTACC 1 cut(s) 2373
AspS9I GGNCC 2 cut(s) 99, 731
AsuC2I CCSGG 2 cut(s) 734, 1471
AsuII TTCGAA 1 cut(s) 2189
AsuNHI GCTAGC 3 cut(s) 1496, 1766, 2060
AvaI CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
BaeGI GKGCMC 1 cut(s) 3242
BanI GGYRCC 2 cut(s) 78, 2373
BanII GRGCYC 4 cut(s) 269, 363, 1886, 3304
BbsI GAAGAC 5 cut(s) 21, 1589, 1769, 1959, 2547
Bbv12I GWGCWC 4 cut(s) 363, 3242, 3259, 3304
BbvCI CCTCAGC 1 cut(s) 741
BbvI GCAGC 9 cut(s) 286, 555, 566, 978, 1000, 1346, 2610, 2815, 2860
BcgI CGANNNNNNTGC 6 cut(s) 60, 94, 681, 715, 3020, 3054
BciT130I CCWGG 2 cut(s) 97, 1073
BclI TGATCA 3 cut(s) 910, 976, 1032
BcnI CCSGG 2 cut(s) 734, 1471
BcoDI GTCTC 9 cut(s) 66, 175, 216, 282, 298, 413, 545, 2463, 3251
BfoI RGCGCY 1 cut(s) 2757
BfuAI ACCTGC 2 cut(s) 167, 549
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 4 cut(s) 1504, 1684, 1864, 2134
BmcAI AGTACT 1 cut(s) 2294
Bme1390I CCNGG 4 cut(s) 97, 734, 1073, 1471
BmeT110I CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
BmgT120I GGNCC 2 cut(s) 99, 731
BmiI GGNNCC 2 cut(s) 80, 2375
BmrFI CCNGG 4 cut(s) 97, 734, 1073, 1471
BmrI ACTGGG 1 cut(s) 2359
BmtI GCTAGC 3 cut(s) 1500, 1770, 2064
BmuI ACTGGG 1 cut(s) 2359
BpiI GAAGAC 5 cut(s) 21, 1589, 1769, 1959, 2547
BplI GAGNNNNNCTC 4 cut(s) 262, 294, 1298, 1330
BpmI CTGGAG 2 cut(s) 172, 1094
Bpu10I CCTNAGC 1 cut(s) 741
Bpu14I TTCGAA 1 cut(s) 2189
BpuEI CTTGAG 1 cut(s) 3233
BpuMI CCSGG 2 cut(s) 734, 1471
BsaAI YACGTR 1 cut(s) 2342
BsaBI GATNNNNATC 1 cut(s) 2865
BsaHI GRCGYC 1 cut(s) 26
BsaJI CCNNGG 6 cut(s) 96, 269, 1025, 1632, 2238, 2932
Bsc4I CCNNNNNNNGG 5 cut(s) 1090, 1357, 2365, 2995, 3173
Bse118I RCCGGY 2 cut(s) 66, 728
Bse1I ACTGG 9 cut(s) 189, 621, 646, 656, 1303, 1377, 2290, 2321, 2354
Bse3DI GCAATG 5 cut(s) 336, 589, 1484, 2798, 3275
Bse8I GATNNNNATC 1 cut(s) 2865
BseBI CCWGG 2 cut(s) 97, 1073
BseDI CCNNGG 6 cut(s) 96, 269, 1025, 1632, 2238, 2932
BseGI GGATG 8 cut(s) 1035, 1479, 2044, 2234, 2287, 2571, 2863, 3319
BseJI GATNNNNATC 1 cut(s) 2865
BseLI CCNNNNNNNGG 5 cut(s) 1090, 1357, 2365, 2995, 3173
BseMI GCAATG 5 cut(s) 336, 589, 1484, 2798, 3275
BseMII CTCAG 9 cut(s) 732, 759, 785, 1022, 1294, 1995, 2147, 2351, 2412
BseNI ACTGG 9 cut(s) 189, 621, 646, 656, 1303, 1377, 2290, 2321, 2354
BseRI GAGGAG 4 cut(s) 324, 1387, 2745, 3152
BseSI GKGCMC 1 cut(s) 3242
BseXI GCAGC 9 cut(s) 286, 555, 566, 978, 1000, 1346, 2610, 2815, 2860
BsgI GTGCAG 1 cut(s) 977
Bsh1236I CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
Bsh1285I CGRYCG 1 cut(s) 1084
BshFI GGCC 6 cut(s) 101, 110, 732, 1313, 2015, 2105
BshNI GGYRCC 2 cut(s) 78, 2373
BsiEI CGRYCG 1 cut(s) 1084
BsiHKAI GWGCWC 4 cut(s) 363, 3242, 3259, 3304
BsiHKCI CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
BsiSI CCGG 5 cut(s) 67, 729, 734, 1222, 1470
BslFI GGGAC 1 cut(s) 710
BslI CCNNNNNNNGG 5 cut(s) 1090, 1357, 2365, 2995, 3173
BsmAI GTCTC 9 cut(s) 66, 175, 216, 282, 298, 413, 545, 2463, 3251
BsmBI CGTCTC 1 cut(s) 298
BsmFI GGGAC 1 cut(s) 710
BsmI GAATGC 1 cut(s) 1468
BsnI GGCC 6 cut(s) 101, 110, 732, 1313, 2015, 2105
BsoBI CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
Bsp119I TTCGAA 1 cut(s) 2189
Bsp1286I GDGCHC 6 cut(s) 269, 363, 1886, 3242, 3259, 3304
Bsp1407I TGTACA 1 cut(s) 3219
BspACI CCGC 7 cut(s) 111, 848, 1114, 1186, 1217, 1490, 1976
BspANI GGCC 6 cut(s) 101, 110, 732, 1313, 2015, 2105
BspCNI CTCAG 9 cut(s) 733, 760, 786, 1021, 1293, 1994, 2148, 2352, 2411
BspFNI CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
BspHI TCATGA 6 cut(s) 955, 1600, 1780, 2050, 2140, 3259
BspLI GGNNCC 2 cut(s) 80, 2375
BspMI ACCTGC 2 cut(s) 167, 549
BspOI GCTAGC 3 cut(s) 1500, 1770, 2064
BspPI GGATC 3 cut(s) 66, 1738, 2327
BspT104I TTCGAA 1 cut(s) 2189
BspT107I GGYRCC 2 cut(s) 78, 2373
BsrDI GCAATG 5 cut(s) 336, 589, 1484, 2798, 3275
BsrFI RCCGGY 2 cut(s) 66, 728
BsrGI TGTACA 1 cut(s) 3219
BsrI ACTGG 9 cut(s) 189, 621, 646, 656, 1303, 1377, 2290, 2321, 2354
BssAI RCCGGY 2 cut(s) 66, 728
BssECI CCNNGG 6 cut(s) 96, 269, 1025, 1632, 2238, 2932
BssNI GRCGYC 1 cut(s) 26
BssT1I CCWWGG 2 cut(s) 2238, 2932
Bst2UI CCWGG 2 cut(s) 97, 1073
Bst4CI ACNGT 4 cut(s) 521, 882, 1595, 2440
Bst6I CTCTTC 3 cut(s) 178, 494, 2989
BstACI GRCGYC 1 cut(s) 26
BstAUI TGTACA 1 cut(s) 3219
BstBAI YACGTR 1 cut(s) 2342
BstBI TTCGAA 1 cut(s) 2189
BstENI CCTNNNNNAGG 1 cut(s) 2363
BstF5I GGATG 8 cut(s) 1035, 1479, 2044, 2234, 2287, 2571, 2863, 3319
BstFNI CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
BstH2I RGCGCY 1 cut(s) 2757
BstMAI GTCTC 9 cut(s) 66, 175, 216, 282, 298, 413, 545, 2463, 3251
BstMCI CGRYCG 1 cut(s) 1084
BstMWI GCNNNNNNNGC 9 cut(s) 32, 107, 1075, 1102, 1174, 2343, 2809, 2870, 3028
BstNI CCWGG 2 cut(s) 97, 1073
BstNSI RCATGY 4 cut(s) 1496, 2634, 3088, 3238
BstSCI CCNGG 4 cut(s) 95, 732, 1071, 1469
BstSLI GKGCMC 1 cut(s) 3242
BstUI CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
BstV1I GCAGC 9 cut(s) 286, 555, 566, 978, 1000, 1346, 2610, 2815, 2860
BstV2I GAAGAC 5 cut(s) 21, 1589, 1769, 1959, 2547
BstX2I RGATCY 4 cut(s) 1504, 1684, 1864, 2134
BstXI CCANNNNNNTGG 2 cut(s) 2239, 2297
BstYI RGATCY 4 cut(s) 1504, 1684, 1864, 2134
BsuRI GGCC 6 cut(s) 101, 110, 732, 1313, 2015, 2105
BtgZI GCGATG 1 cut(s) 2793
BtsCI GGATG 8 cut(s) 1035, 1479, 2044, 2234, 2287, 2571, 2863, 3319
BtsI GCAGTG 1 cut(s) 1200
BveI ACCTGC 2 cut(s) 167, 549
CciI TCATGA 6 cut(s) 955, 1600, 1780, 2050, 2140, 3259
Cfr10I RCCGGY 2 cut(s) 66, 728
Cfr13I GGNCC 2 cut(s) 99, 731
CseI GACGC 2 cut(s) 15, 1109
Csp6I GTAC 7 cut(s) 924, 1214, 1382, 1842, 2293, 2374, 3220
CspCI CAANNNNNGTGG 4 cut(s) 1542, 1577, 1632, 1667
CviQI GTAC 7 cut(s) 924, 1214, 1382, 1842, 2293, 2374, 3220
DraI TTTAAA 1 cut(s) 1803
DrdI GACNNNNNNGTC 1 cut(s) 559
DseDI GACNNNNNNGTC 1 cut(s) 559
Eam1104I CTCTTC 3 cut(s) 178, 494, 2989
EarI CTCTTC 3 cut(s) 178, 494, 2989
Ecl136II GAGCTC 2 cut(s) 361, 3302
Eco130I CCWWGG 2 cut(s) 2238, 2932
Eco147I AGGCCT 1 cut(s) 1313
Eco24I GRGCYC 4 cut(s) 269, 363, 1886, 3304
Eco32I GATATC 2 cut(s) 853, 2551
Eco47III AGCGCT 1 cut(s) 2755
Eco53kI GAGCTC 2 cut(s) 361, 3302
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 5 cut(s) 268, 279, 1705, 1795, 1885
EcoICRI GAGCTC 2 cut(s) 361, 3302
EcoNI CCTNNNNNAGG 1 cut(s) 2363
EcoRI GAATTC 2 cut(s) 1149, 2999
EcoRII CCWGG 2 cut(s) 95, 1071
EcoRV GATATC 2 cut(s) 853, 2551
EcoT14I CCWWGG 2 cut(s) 2238, 2932
EcoT22I ATGCAT 2 cut(s) 934, 1278
EcoT38I GRGCYC 4 cut(s) 269, 363, 1886, 3304
ErhI CCWWGG 2 cut(s) 2238, 2932
Esp3I CGTCTC 1 cut(s) 298
FaqI GGGAC 1 cut(s) 710
FauI CCCGC 1 cut(s) 1983
FbaI TGATCA 3 cut(s) 910, 976, 1032
FblI GTMKAC 3 cut(s) 1110, 3081, 3207
FokI GGATG 8 cut(s) 1042, 1486, 2031, 2221, 2294, 2578, 2870, 3326
FriOI GRGCYC 4 cut(s) 269, 363, 1886, 3304
FspI TGCGCA 1 cut(s) 2211
GsuI CTGGAG 2 cut(s) 172, 1094
HaeII RGCGCY 1 cut(s) 2757
HaeIII GGCC 6 cut(s) 101, 110, 732, 1313, 2015, 2105
HapII CCGG 5 cut(s) 67, 729, 734, 1222, 1470
HgaI GACGC 2 cut(s) 15, 1109
Hin1I GRCGYC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 3208
HindII GTYRAC 1 cut(s) 3208
HpaII CCGG 5 cut(s) 67, 729, 734, 1222, 1470
HpyCH4III ACNGT 4 cut(s) 521, 882, 1595, 2440
HpyCH4IV ACGT 2 cut(s) 1440, 2341
HpyF10VI GCNNNNNNNGC 9 cut(s) 32, 107, 1075, 1102, 1174, 2343, 2809, 2870, 3028
HpySE526I ACGT 2 cut(s) 1440, 2341
Hsp92I GRCGYC 1 cut(s) 26
KpnI GGTACC 1 cut(s) 2377
Ksp22I TGATCA 3 cut(s) 910, 976, 1032
LmnI GCTCC 9 cut(s) 470, 1075, 1093, 1306, 1400, 2832, 3028, 3052, 3307
Lsp1109I GCAGC 9 cut(s) 286, 555, 566, 978, 1000, 1346, 2610, 2815, 2860
MaeII ACGT 2 cut(s) 1440, 2341
MaeIII GTNAC 9 cut(s) 191, 1436, 1537, 1589, 1627, 1807, 1897, 1987, 2927
MfeI CAATTG 3 cut(s) 2573, 2618, 3306
MflI RGATCY 4 cut(s) 1504, 1684, 1864, 2134
MhlI GDGCHC 6 cut(s) 269, 363, 1886, 3242, 3259, 3304
MlyI GAGTC 5 cut(s) 415, 644, 692, 1744, 3203
MmeI TCCRAC 3 cut(s) 448, 2418, 3030
Mph1103I ATGCAT 2 cut(s) 934, 1278
MseI TTAA 5 cut(s) 858, 1170, 1431, 1802, 3069
MslI CAYNNNNRTG 9 cut(s) 368, 1193, 1301, 1857, 1947, 2037, 2049, 2127, 3089
MspA1I CMGCKG 2 cut(s) 579, 991
MspI CCGG 5 cut(s) 67, 729, 734, 1222, 1470
MspR9I CCNGG 4 cut(s) 97, 734, 1073, 1471
MunI CAATTG 3 cut(s) 2573, 2618, 3306
Mva1269I GAATGC 1 cut(s) 1468
MvaI CCWGG 2 cut(s) 97, 1073
MvnI CGCG 5 cut(s) 951, 1080, 1103, 1219, 1978
MwoI GCNNNNNNNGC 9 cut(s) 32, 107, 1075, 1102, 1174, 2343, 2809, 2870, 3028
NciI CCSGG 2 cut(s) 734, 1471
NheI GCTAGC 3 cut(s) 1496, 1766, 2060
NlaIV GGNNCC 2 cut(s) 80, 2375
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 8 cut(s) 191, 1436, 1537, 1627, 1807, 1897, 1987, 2927
NsbI TGCGCA 1 cut(s) 2211
NsiI ATGCAT 2 cut(s) 934, 1278
NspI RCATGY 4 cut(s) 1496, 2634, 3088, 3238
NspV TTCGAA 1 cut(s) 2189
OliI CACNNNNGTG 1 cut(s) 1301
PaeI GCATGC 2 cut(s) 1496, 2634
PagI TCATGA 6 cut(s) 955, 1600, 1780, 2050, 2140, 3259
PaqCI CACCTGC 1 cut(s) 167
PceI AGGCCT 1 cut(s) 1313
PciI ACATGT 1 cut(s) 3234
PcsI WCGNNNNNNNCGW 1 cut(s) 401
PctI GAATGC 1 cut(s) 1468
PfeI GAWTC 6 cut(s) 145, 573, 617, 746, 2492, 2569
PleI GAGTC 5 cut(s) 415, 643, 692, 1743, 3203
PpsI GAGTC 5 cut(s) 415, 643, 692, 1743, 3203
Ppu21I YACGTR 1 cut(s) 2342
PscI ACATGT 1 cut(s) 3234
PsiI TTATAA 1 cut(s) 1016
Psp124BI GAGCTC 2 cut(s) 363, 3304
Psp6I CCWGG 2 cut(s) 95, 1071
PspGI CCWGG 2 cut(s) 95, 1071
PspN4I GGNNCC 2 cut(s) 80, 2375
PspPI GGNCC 2 cut(s) 99, 731
PsuI RGATCY 4 cut(s) 1504, 1684, 1864, 2134
PvuII CAGCTG 2 cut(s) 579, 991
RsaI GTAC 7 cut(s) 925, 1215, 1383, 1843, 2294, 2375, 3221
RsaNI GTAC 7 cut(s) 924, 1214, 1382, 1842, 2293, 2374, 3220
RseI CAYNNNNRTG 9 cut(s) 368, 1193, 1301, 1857, 1947, 2037, 2049, 2127, 3089
SacI GAGCTC 2 cut(s) 363, 3304
SalI GTCGAC 1 cut(s) 3206
SaqAI TTAA 5 cut(s) 858, 1170, 1431, 1802, 3069
Sau96I GGNCC 2 cut(s) 99, 731
ScaI AGTACT 1 cut(s) 2294
SchI GAGTC 5 cut(s) 415, 644, 692, 1744, 3203
ScrFI CCNGG 4 cut(s) 97, 734, 1073, 1471
SduI GDGCHC 6 cut(s) 269, 363, 1886, 3242, 3259, 3304
SfiI GGCCNNNNNGGCC 1 cut(s) 107
SfuI TTCGAA 1 cut(s) 2189
SmiMI CAYNNNNRTG 9 cut(s) 368, 1193, 1301, 1857, 1947, 2037, 2049, 2127, 3089
SmlI CTYRAG 1 cut(s) 3248
SmoI CTYRAG 1 cut(s) 3248
SphI GCATGC 2 cut(s) 1496, 2634
SseBI AGGCCT 1 cut(s) 1313
SsiI CCGC 7 cut(s) 111, 848, 1114, 1186, 1217, 1490, 1976
SstI GAGCTC 2 cut(s) 363, 3304
StuI AGGCCT 1 cut(s) 1313
StyD4I CCNGG 4 cut(s) 95, 732, 1071, 1469
StyI CCWWGG 2 cut(s) 2238, 2932
TaaI ACNGT 4 cut(s) 521, 882, 1595, 2440
TaiI ACGT 2 cut(s) 1443, 2344
TaqII GACCGA 2 cut(s) 234, 1098
TatI WGTACW 3 cut(s) 1381, 2292, 3219
TauI GCSGC 3 cut(s) 113, 1188, 1492
TfiI GAWTC 6 cut(s) 145, 573, 617, 746, 2492, 2569
Tru1I TTAA 5 cut(s) 858, 1170, 1431, 1802, 3069
Tru9I TTAA 5 cut(s) 858, 1170, 1431, 1802, 3069
TseFI GTSAC 8 cut(s) 191, 1436, 1537, 1627, 1807, 1897, 1987, 2927
TseI GCWGC 9 cut(s) 299, 543, 579, 988, 991, 1334, 2623, 2803, 2873
Tsp45I GTSAC 8 cut(s) 191, 1436, 1537, 1627, 1807, 1897, 1987, 2927
TspGWI ACGGA 2 cut(s) 449, 927
VneI GTGCAC 1 cut(s) 3238
XagI CCTNNNNNAGG 1 cut(s) 2363
XapI RAATTY 4 cut(s) 124, 450, 1149, 2999
XbaI TCTAGA 1 cut(s) 941
XceI RCATGY 4 cut(s) 1496, 2634, 3088, 3238
XcmI CCANNNNNNNNNTGG 1 cut(s) 2346
XmiI GTMKAC 3 cut(s) 1110, 3081, 3207
ZrmI AGTACT 1 cut(s) 2294
Zsp2I ATGCAT 2 cut(s) 934, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.