RLG00000032625

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0011156
Physical Location & Seq
Forward (+)
17083167 .. 17088620
5454 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032625

Sequence Viewer

Length: 4065 bp
ATGGCATCTTCCCAAGTGGAAATGGCCTCTGCGCCGTTCGGTTGCGTTTTGAGGGATCACAACCGCCGGGACCGGTGCAGAGAGAGCAATGCCAGAGCCACCATAGGTGTTCTGCAGAAGAATCTCAAGAGTTTGGTGAGGGATCATCTCTGCATTGCAGTGTCTTCAAACTCTGGCTCCAATGACAGCGACTCCGAGAACCAGGGTCGAAACAAGGAGCCAAAAAAGGATGAGAACAGCAACAGAAATGGTGAAAGAGAAGATGGTTCGGGTTCGGTTATGAGCCCGAGGCATGCTAGGGTTCTTGATCGATGGGCTTTCGCAAGACAAATAGTGACGAAACCAATTGAGAGGCAGAGGAGAGAAGAGGCAGAGGTGGTTTCTGTGCCGAACTCGGCTCCTACTTCAACAAGGACGTCGACTTCTGCCAAGGAAGAGACTATTGCAAGAACAGATAGCTTGGCTGATCTCACCAAAAAAGGCGTGGGGGCTTCGTCTCTGGTTCAAATTTGGGAGAAGAGGCTCCACAAATCAGAATCCCTCAAAACCAATTCCAATGGAAATGTAATATCCGAGACAAGTAGGTCAGATTCTGGAGTTAGCTGCCCTGAGAATGAGAATGAGAATGTAATCGTTCCCAATGCTTCACCGTCAAGAGTTTCTGAAGCAGAAAAGGCAGAGTCCCCTGATGAGAAAGCAGGGGATACTGTTTTCTCCATTGAAAAAACAGGGGACTCTGTTTTGGATATGGAAAAAGCAAGGGAGTCTATTGATGAAAGCTATGATGCCGGCGCTGTTACCGACATTGAAGATTCTGATAGGATTGCTGTGAATAGCAGGAATTTAGATTCTAAAGAAAAAGAGAAGGTTCGAGTTGTGGATATTATTAGAAGACTGACTGCACAGGGTCCATCGTCCTCTATGAGTGAGGAAAATGCTAATGATCATGATCAGCAGTTTAGTAGCGCTCACGGATCGCCATGTAGGGATAACCCCCGTGAGCTGTTACGTGATCGGTGTTCTGGGGTTGATCAAGCAGAGCACAGAGGCCTCTCTCATGTTATAAGCTCACCGCGCATCAGAGGGAGGCAGGCATTTGCCGATTTGCTTATGCAGATGGAGCGTGACAGGCATAGGGAACTTGACACACTGGTAAACCGCAAAACAGTTTCTAAGTTCCCACAGCGAGGCCGAACTCGAATTCAGTCATTACTTAGGCTTCGATTGCTTCAACGTGGTGTGGCATTTGCGGGTGTAGATGTGCCAACACTTCCTCCAAAAGCATCTCGTGTGAACAAACCGGCGCAAGGATCTAGCATCATGCATCTCAGGGAGAAATTTCGTACTAGTGCTGAGCATGGTTCAGTAGGGCAGAGTGATGCACCTAATCCAAATAGCCCTCATAAGGAGATCATAAAGAACCAAAGTGTCAATGTAGACAAATCCTCCACCTCCAATAAGCCTAGTGAAAACACTCCTAATCGAGAAGTTAATGTTATGGAGCAGCAGAAAACATTCCCAGTACTGCGTGCTAGTGAAGATGTTTGTGAAGAAGCGACCCCCAATTTACACAAATCCTCCACCTCCAATAAGCCTAGTGAAAGAACTCCTAATCGAGAAGTTAATGTTATGGAACAGCAGAAAGCATCCCCAGTACTGCGTACTAGTGAAGATGTTTGTGAAGAAGCGACCCAGAGTTTACACAAGTCCTCCACCTCCAATAAGCCCAGTGAAAGCACTCCTAATCGAGAAGTTAATGTTATGGAGCAGCAGAAAATATTCCCAGTACTGCGTGCTAGTGAAGATGTTTGTGAAGAAGCGACCCCCAGTTTACACAAATCCTCCACCTCTAATAAGCCTAGTGAAAGCACTCCTAATCGAGAAATTAATGTTATGGAGCAGCAGAAAGCATCCCCAGTACTGCGTGCTAGTGAAGATGTTTGTGAAGAAGCGACCCGGAGTTTACAGAAATCCTCCACCTCCAATAAGCCTAGTGAAAGCACTCCTAATCGAGAAGTTAATGTTATGGAGCAGCAGAAAACATCCCCAGTACTGCGTGCTAGTGAAGAAGCTATCCCCAGTTTACACAAATCCTCCACCTCCAATAAACCTAGTGAAAGCTTCCCTAATCGAGAAGTTAATGATATGGAGCAGCAGAAAGCATCCCCAGTACTGCGTGCTAGTGAGGATGTTTGTGAAGAAGTGACCCCCAGTTTAGAAGTTTGCTTCCAAGAAACTAGTTCAGTTTCTCAAAGCACCTTGCCAAATGTTAGTGAAGATCTTTGTGAAGTAGCCAGCCCAAGTCCGAAGGTCACCTGGCAAGACACAAGTTCAGTAGCTAGAAACCTTGGTTCCCAAGAAACTGCTGATCCAACCACACCCATAAATGGTTCAAATGAGAGTGAGATATGGAATGGAAAAGGTTCAGTGGATAGAAACCTTGATTCACAAGAAACTGTAGATTCAACAACATCCTTGAATGGTCGCGATGGGGATGATGAAATATGGAAGCCCACAAGTTCAGAGGCTAGATACCTTGATTCACAACAAAGTGCAGATACAAGGAAACCCTTGAGTGGTGGGGATGGAACTGGGAATGAAATATGGAAAGAAACAAGTTCAGGGGCTAGAAACCTTGACACACATGAAGCTGCAGATACAACCTCATCCTTGAATGGTTGGAATGAAAATGCGATATGGCATGGAACAAGTTTAGTGGCTAGAAACTTTGACTCACAAGTAGATACAACAACATCCTTGGTCGACTGGGATGAGAATGATGATGATTACCAGTATTACGGAGAAACAAATTATGACTGGATCAGTGAGATTTCTCGCCCACGGAGCTATTGGGAAGACATTAGGAAATCATGGTACCAAGAGATGCTTAACTCCAATTCAGAAAAAGGCGAGATACACCAACTCCTTGAAAGAAGAACAGTATCAAACTTTCTTTCTAGTGATTTCCGAGATAGGATAGATAGATTGATGGTATCTCGCATAGAAAGACAAACATGTCACAATGTCAGTCAAGAAGAAGAAGATGACGAGAGCAGCTATGATAGAATGAATCAAATGGTGTCTTTCTTACAAAAACGCAGCAGCCCATCAGGCACTGAAGAAGTAGAAGAAGTGCAAGAACATGTACAGGTACAAGAACAAAACCAAGTCCTAGAACAAGTGCTAGAACACGTGCAAGTCCAAGAACAGGTGCGAGAACACGTGCAAGTCCAAGAACAGGTGCGAGAACATCAAGACCATGAAGACGAGGAGATGACAGAAGAAGAGGAGGAGCAAGAAGAAGCAGAAGAAGCGAAAGAACATGTGCAGGTACTAGAACAAAACCAAGTCCAAGAACAAGTGCTAGAACAGGTGCAAGAACATCAAGACCACGAGGACGAGGGGATGACAGAAGGAGAAAATGATGACAACTACGATGATGATGAAGAATCAAGGAGCCTAATTAGTGGTCAGTTTCAAGAAGCTAGTGATTATTTTGACCGTGCATCATTGCAGCCTTCACCATCTCACTTTACAACATGGAGTTACCAAGATAATGAAGTTGGGGATGATTCGGACCAAGCCACTTCTCCATCTCCTCGCCAACGGTCACCCTTTCCATCTTACTATCGAAATAGTCCACAACTTTCACCATCTCCATCTTACAACGCTAATCACCGGCAACGTTCTTCCTCCCTCAGTCATCCTTCAATGGAAATGGAGCTCATATACAACTTGAGAGGGCATATGATACAACTCTATCAAGAGATGTCTGAGCTAAGAAAAGAAATAAAGAGCTGTGTAGACATGCAAATGAAAGTGCAACAATCCATGAACCAGGATGTTCATTCAGGTCAAGTGGGGATGAAAAGAAACTCTAGTGGGTTAACAAAGAAAGGCAATTGCCGCATTTGCAATGAAGCAAAAATCGACTCACTTTTGTACAGGTGTGGACACATGTGCACCTGTCTCAAGTGTGCTCATGATTTGCAATGGAACAGCGGAAAATGTCCAATCTGTCGAGCTCCGATTGCAGATGTCGTAAGGGCATACATGGATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

1355

Amino Acids

152.18

Weight (kDa)

4.99

Isoelectric Point (pI)

61.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 1299 - 1346 3.2e-13 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1064
AasI GACNNNNNNGTC 2 cut(s) 583, 3015
AatII GACGTC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 3319
Acc65I GGTACC 1 cut(s) 2874
AccB1I GGYRCC 1 cut(s) 2874
AccI GTMKAC 4 cut(s) 419, 1437, 2763, 3804
AccII CGCG 2 cut(s) 1075, 2487
AciI CCGC 6 cut(s) 64, 1073, 1159, 1250, 3907, 4002
AclI AACGTT 1 cut(s) 3685
AclWI GGATC 6 cut(s) 63, 150, 982, 1318, 2363, 2828
AcsI RAATTY 4 cut(s) 507, 841, 1200, 1337
AcuI CTGAAG 2 cut(s) 684, 3138
AcvI CACGTG 2 cut(s) 3193, 3223
AcyI GRCGYC 1 cut(s) 416
AfeI AGCGCT 1 cut(s) 967
AfiI CCNNNNNNNGG 7 cut(s) 1187, 1307, 1405, 2387, 2576, 3208, 3238
AflIII ACRYGT 6 cut(s) 3014, 3142, 3190, 3220, 3322, 3957
AgeI ACCGGT 1 cut(s) 72
AhlI ACTAGT 3 cut(s) 1346, 1664, 2237
AjnI CCWGG 3 cut(s) 201, 2315, 3837
Alw21I GWGCWC 5 cut(s) 1044, 3726, 3965, 3982, 4027
Alw26I GTCTC 4 cut(s) 431, 501, 569, 3974
Alw44I GTGCAC 1 cut(s) 3961
AlwI GGATC 6 cut(s) 63, 150, 982, 1318, 2363, 2828
AlwNI CAGNNNCTG 2 cut(s) 593, 3116
Ama87I CYCGRG 1 cut(s) 286
Aor51HI AGCGCT 1 cut(s) 967
AoxI GGCC 3 cut(s) 24, 1048, 1189
ApaLI GTGCAC 1 cut(s) 3961
ApoI RAATTY 4 cut(s) 507, 841, 1200, 1337
AseI ATTAAT 1 cut(s) 1887
AsiGI ACCGGT 1 cut(s) 72
Asp700I GAANNNNTTC 3 cut(s) 1514, 1778, 2422
Asp718I GGTACC 1 cut(s) 2874
AspLEI GCGC 5 cut(s) 34, 794, 968, 1077, 1306
AspS9I GGNCC 3 cut(s) 70, 908, 3577
AsuC2I CCSGG 2 cut(s) 68, 1957
AvaI CYCGRG 1 cut(s) 286
AvaII GGWCC 3 cut(s) 70, 908, 3577
BaeGI GKGCMC 1 cut(s) 3965
BanI GGYRCC 1 cut(s) 2874
BanII GRGCYC 3 cut(s) 287, 3726, 4027
BauI CACGAG 2 cut(s) 1287, 3392
BbrPI CACGTG 2 cut(s) 3193, 3223
BbsI GAAGAC 4 cut(s) 156, 898, 2862, 3270
Bbv12I GWGCWC 5 cut(s) 1044, 3726, 3965, 3982, 4027
BceAI ACGGC 1 cut(s) 19
BciT130I CCWGG 3 cut(s) 203, 2317, 3839
BciVI GTATCC 1 cut(s) 697
BclI TGATCA 3 cut(s) 943, 949, 1030
BcnI CCSGG 2 cut(s) 68, 1957
BcoDI GTCTC 4 cut(s) 431, 501, 569, 3974
BcuI ACTAGT 3 cut(s) 1346, 1664, 2237
BfmI CTRYAG 3 cut(s) 113, 2457, 2652
BfoI RGCGCY 2 cut(s) 795, 969
BfuAI ACCTGC 1 cut(s) 3319
BfuI GTATCC 1 cut(s) 697
BglI GCCNNNNNGGC 1 cut(s) 3111
BglII AGATCT 1 cut(s) 2278
BlpI GCTNAGC 1 cut(s) 1353
BmcAI AGTACT 6 cut(s) 1524, 1656, 1788, 1920, 2052, 2172
Bme1390I CCNGG 5 cut(s) 68, 203, 1957, 2317, 3839
Bme18I GGWCC 3 cut(s) 70, 908, 3577
BmeT110I CYCGRG 1 cut(s) 286
BmgT120I GGNCC 3 cut(s) 70, 908, 3577
BmiI GGNNCC 9 cut(s) 71, 178, 219, 399, 524, 909, 2353, 2876, 3458
BmrFI CCNGG 5 cut(s) 68, 203, 1957, 2317, 3839
BpiI GAAGAC 4 cut(s) 156, 898, 2862, 3270
BpmI CTGGAG 1 cut(s) 615
Bpu1102I GCTNAGC 1 cut(s) 1353
BpuEI CTTGAG 4 cut(s) 110, 2593, 3757, 3956
BpuMI CCSGG 2 cut(s) 68, 1957
Bsa29I ATCGAT 1 cut(s) 310
BsaAI YACGTR 3 cut(s) 1010, 3193, 3223
BsaBI GATNNNNATC 1 cut(s) 948
BsaHI GRCGYC 1 cut(s) 416
BsaJI CCNNGG 6 cut(s) 202, 287, 429, 2347, 2757, 2840
BsaWI WCCGGW 1 cut(s) 72
Bsc4I CCNNNNNNNGG 7 cut(s) 1187, 1307, 1405, 2387, 2576, 3208, 3238
Bse118I RCCGGY 4 cut(s) 72, 788, 1300, 3678
Bse3DI GCAATG 5 cut(s) 94, 153, 3509, 3922, 3998
Bse8I GATNNNNATC 1 cut(s) 948
BseBI CCWGG 3 cut(s) 203, 2317, 3839
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 6 cut(s) 202, 287, 429, 2347, 2757, 2840
BseJI GATNNNNATC 1 cut(s) 948
BseLI CCNNNNNNNGG 7 cut(s) 1187, 1307, 1405, 2387, 2576, 3208, 3238
BseMI GCAATG 5 cut(s) 94, 153, 3509, 3922, 3998
BseMII CTCAG 5 cut(s) 600, 1342, 1344, 3712, 3765
BseRI GAGGAG 5 cut(s) 373, 3284, 3302, 3305, 3588
BseSI GKGCMC 1 cut(s) 3965
BsgI GTGCAG 4 cut(s) 97, 885, 2574, 3347
Bsh1236I CGCG 2 cut(s) 1075, 2487
BshFI GGCC 3 cut(s) 26, 1050, 1191
BshNI GGYRCC 1 cut(s) 2874
BshTI ACCGGT 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 5 cut(s) 1044, 3726, 3965, 3982, 4027
BsiHKCI CYCGRG 1 cut(s) 286
BsiSI CCGG 6 cut(s) 67, 73, 789, 1301, 1957, 3679
BslFI GGGAC 3 cut(s) 83, 667, 746
BslI CCNNNNNNNGG 7 cut(s) 1187, 1307, 1405, 2387, 2576, 3208, 3238
BsmAI GTCTC 4 cut(s) 431, 501, 569, 3974
BsmBI CGTCTC 1 cut(s) 501
BsmFI GGGAC 3 cut(s) 83, 667, 746
BsnI GGCC 3 cut(s) 26, 1050, 1191
BsoBI CYCGRG 1 cut(s) 286
Bsp1286I GDGCHC 6 cut(s) 287, 1044, 3726, 3965, 3982, 4027
Bsp1407I TGTACA 2 cut(s) 3145, 3942
Bsp1720I GCTNAGC 1 cut(s) 1353
Bsp68I TCGCGA 1 cut(s) 2487
BspACI CCGC 6 cut(s) 64, 1073, 1159, 1250, 3907, 4002
BspANI GGCC 3 cut(s) 26, 1050, 1191
BspCNI CTCAG 5 cut(s) 601, 1341, 1345, 3711, 3766
BspDI ATCGAT 1 cut(s) 310
BspFNI CGCG 2 cut(s) 1075, 2487
BspHI TCATGA 2 cut(s) 946, 3982
BspLI GGNNCC 9 cut(s) 71, 178, 219, 399, 524, 909, 2353, 2876, 3458
BspMAI CTGCAG 2 cut(s) 117, 2656
BspMI ACCTGC 1 cut(s) 3319
BspPI GGATC 6 cut(s) 63, 150, 982, 1318, 2363, 2828
BspT107I GGYRCC 1 cut(s) 2874
BsrDI GCAATG 5 cut(s) 94, 153, 3509, 3922, 3998
BsrFI RCCGGY 4 cut(s) 72, 788, 1300, 3678
BsrGI TGTACA 2 cut(s) 3145, 3942
BssAI RCCGGY 4 cut(s) 72, 788, 1300, 3678
BssECI CCNNGG 6 cut(s) 202, 287, 429, 2347, 2757, 2840
BssNI GRCGYC 1 cut(s) 416
BssSI CACGAG 2 cut(s) 1287, 3392
BssT1I CCWWGG 3 cut(s) 429, 2347, 2757
Bst2BI CACGAG 2 cut(s) 1287, 3392
Bst2UI CCWGG 3 cut(s) 203, 2317, 3839
Bst4CI ACNGT 7 cut(s) 651, 709, 1168, 2458, 2941, 3503, 3609
Bst6I CTCTTC 4 cut(s) 360, 429, 512, 3279
BstACI GRCGYC 1 cut(s) 416
BstAUI TGTACA 2 cut(s) 3145, 3942
BstBAI YACGTR 3 cut(s) 1010, 3193, 3223
BstC8I GCNNGC 9 cut(s) 294, 790, 1092, 1530, 1794, 1926, 2058, 2178, 2296
BstDEI CTNAG 8 cut(s) 609, 1173, 1214, 1328, 1353, 3698, 3774, 3779
BstDSI CCRYGG 1 cut(s) 2840
BstEII GGTNACC 2 cut(s) 2311, 3609
BstFNI CGCG 2 cut(s) 1075, 2487
BstH2I RGCGCY 2 cut(s) 795, 969
BstHHI GCGC 5 cut(s) 34, 794, 968, 1077, 1306
BstMAI GTCTC 4 cut(s) 431, 501, 569, 3974
BstNI CCWGG 3 cut(s) 203, 2317, 3839
BstNSI RCATGY 6 cut(s) 296, 3018, 3146, 3326, 3811, 3961
BstPI GGTNACC 2 cut(s) 2311, 3609
BstSCI CCNGG 5 cut(s) 66, 201, 1955, 2315, 3837
BstSFI CTRYAG 3 cut(s) 113, 2457, 2652
BstSLI GKGCMC 1 cut(s) 3965
BstUI CGCG 2 cut(s) 1075, 2487
BstV2I GAAGAC 4 cut(s) 156, 898, 2862, 3270
BstX2I RGATCY 2 cut(s) 1310, 2278
BstYI RGATCY 2 cut(s) 1310, 2278
Bsu15I ATCGAT 1 cut(s) 310
BsuI GTATCC 1 cut(s) 697
BsuRI GGCC 3 cut(s) 26, 1050, 1191
BsuTUI ATCGAT 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 2840
BtgZI GCGATG 1 cut(s) 2502
BtsI GCAGTG 1 cut(s) 165
BtsIMutI CAGTG 6 cut(s) 165, 1148, 1735, 2433, 2830, 3114
BtuMI TCGCGA 1 cut(s) 2487
BveI ACCTGC 1 cut(s) 3319
Cac8I GCNNGC 9 cut(s) 294, 790, 1092, 1530, 1794, 1926, 2058, 2178, 2296
CaiI CAGNNNCTG 2 cut(s) 593, 3116
CciI TCATGA 2 cut(s) 946, 3982
CfoI GCGC 5 cut(s) 34, 794, 968, 1077, 1306
Cfr10I RCCGGY 4 cut(s) 72, 788, 1300, 3678
Cfr13I GGNCC 3 cut(s) 70, 908, 3577
ClaI ATCGAT 1 cut(s) 310
CspAI ACCGGT 1 cut(s) 72
CspCI CAANNNNNGTGG 2 cut(s) 2697, 2732
DdeI CTNAG 8 cut(s) 609, 1173, 1214, 1328, 1353, 3698, 3774, 3779
DrdI GACNNNNNNGTC 2 cut(s) 583, 3015
DseDI GACNNNNNNGTC 2 cut(s) 583, 3015
Eam1104I CTCTTC 4 cut(s) 360, 429, 512, 3279
EarI CTCTTC 4 cut(s) 360, 429, 512, 3279
Ecl136II GAGCTC 2 cut(s) 3724, 4025
Eco130I CCWWGG 3 cut(s) 429, 2347, 2757
Eco147I AGGCCT 1 cut(s) 1050
Eco24I GRGCYC 3 cut(s) 287, 3726, 4027
Eco47I GGWCC 3 cut(s) 70, 908, 3577
Eco47III AGCGCT 1 cut(s) 967
Eco53kI GAGCTC 2 cut(s) 3724, 4025
Eco57I CTGAAG 2 cut(s) 684, 3138
Eco72I CACGTG 2 cut(s) 3193, 3223
Eco88I CYCGRG 1 cut(s) 286
Eco91I GGTNACC 2 cut(s) 2311, 3609
EcoICRI GAGCTC 2 cut(s) 3724, 4025
EcoO65I GGTNACC 2 cut(s) 2311, 3609
EcoRI GAATTC 1 cut(s) 1200
EcoRII CCWGG 3 cut(s) 201, 2315, 3837
EcoT14I CCWWGG 3 cut(s) 429, 2347, 2757
EcoT22I ATGCAT 1 cut(s) 1326
EcoT38I GRGCYC 3 cut(s) 287, 3726, 4027
ErhI CCWWGG 3 cut(s) 429, 2347, 2757
Esp3I CGTCTC 1 cut(s) 501
FalI AAGNNNNNCTT 2 cut(s) 2871, 2903
FaqI GGGAC 3 cut(s) 83, 667, 746
FauI CCCGC 1 cut(s) 1243
FauNDI CATATG 1 cut(s) 3747
FbaI TGATCA 3 cut(s) 943, 949, 1030
FblI GTMKAC 4 cut(s) 419, 1437, 2763, 3804
FriOI GRGCYC 3 cut(s) 287, 3726, 4027
GlaI GCGC 5 cut(s) 33, 793, 967, 1076, 1305
GsuI CTGGAG 1 cut(s) 615
HaeII RGCGCY 2 cut(s) 795, 969
HaeIII GGCC 3 cut(s) 26, 1050, 1191
HapII CCGG 6 cut(s) 67, 73, 789, 1301, 1957, 3679
HhaI GCGC 5 cut(s) 34, 794, 968, 1077, 1306
Hin1I GRCGYC 1 cut(s) 416
Hin6I GCGC 5 cut(s) 32, 792, 966, 1075, 1304
HinP1I GCGC 5 cut(s) 32, 792, 966, 1075, 1304
HincII GTYRAC 3 cut(s) 420, 2764, 3888
HindII GTYRAC 3 cut(s) 420, 2764, 3888
HindIII AAGCTT 1 cut(s) 2119
HpaI GTTAAC 1 cut(s) 3888
HpaII CCGG 6 cut(s) 67, 73, 789, 1301, 1957, 3679
Hpy99I CGWCG 1 cut(s) 421
HpyAV CCTTC 5 cut(s) 859, 2302, 3407, 3528, 3717
HpyCH4III ACNGT 7 cut(s) 651, 709, 1168, 2458, 2941, 3503, 3609
HpyCH4IV ACGT 6 cut(s) 416, 1009, 1234, 3192, 3222, 3685
HpyF3I CTNAG 8 cut(s) 609, 1173, 1214, 1328, 1353, 3698, 3774, 3779
HpySE526I ACGT 6 cut(s) 416, 1009, 1234, 3192, 3222, 3685
Hsp92I GRCGYC 1 cut(s) 416
HspAI GCGC 5 cut(s) 32, 792, 966, 1075, 1304
KpnI GGTACC 1 cut(s) 2878
KroI GCCGGC 1 cut(s) 788
KroNI GCCGGC 1 cut(s) 790
Ksp22I TGATCA 3 cut(s) 943, 949, 1030
KspAI GTTAAC 1 cut(s) 3888
MaeII ACGT 6 cut(s) 416, 1009, 1234, 3192, 3222, 3685
MaeIII GTNAC 9 cut(s) 334, 796, 1005, 1124, 2203, 2311, 3017, 3545, 3609
MfeI CAATTG 2 cut(s) 345, 3901
MflI RGATCY 2 cut(s) 1310, 2278
MhlI GDGCHC 6 cut(s) 287, 1044, 3726, 3965, 3982, 4027
MlyI GAGTC 6 cut(s) 185, 689, 728, 773, 2726, 3926
MmeI TCCRAC 2 cut(s) 2396, 2660
Mph1103I ATGCAT 1 cut(s) 1326
MroNI GCCGGC 1 cut(s) 788
MroXI GAANNNNTTC 3 cut(s) 1514, 1778, 2422
MseI TTAA 9 cut(s) 1491, 1623, 1755, 1887, 2019, 2139, 2889, 3887, 4063
MslI CAYNNNNRTG 2 cut(s) 158, 3812
MspA1I CMGCKG 1 cut(s) 4002
MspI CCGG 6 cut(s) 67, 73, 789, 1301, 1957, 3679
MspR9I CCNGG 5 cut(s) 68, 203, 1957, 2317, 3839
MunI CAATTG 2 cut(s) 345, 3901
MvaI CCWGG 3 cut(s) 203, 2317, 3839
MvnI CGCG 2 cut(s) 1075, 2487
NaeI GCCGGC 1 cut(s) 790
NciI CCSGG 2 cut(s) 68, 1957
NdeI CATATG 1 cut(s) 3747
NgoMIV GCCGGC 1 cut(s) 788
NlaIV GGNNCC 9 cut(s) 71, 178, 219, 399, 524, 909, 2353, 2876, 3458
NmeAIII GCCGAG 1 cut(s) 374
NmuCI GTSAC 6 cut(s) 334, 1124, 2203, 2311, 3017, 3609
NruI TCGCGA 1 cut(s) 2487
NsiI ATGCAT 1 cut(s) 1326
NspI RCATGY 6 cut(s) 296, 3018, 3146, 3326, 3811, 3961
PaeI GCATGC 1 cut(s) 296
PagI TCATGA 2 cut(s) 946, 3982
PceI AGGCCT 1 cut(s) 1050
PciI ACATGT 4 cut(s) 3014, 3142, 3322, 3957
PdiI GCCGGC 1 cut(s) 790
PdmI GAANNNNTTC 3 cut(s) 1514, 1778, 2422
PinAI ACCGGT 1 cut(s) 72
PleI GAGTC 6 cut(s) 185, 688, 728, 772, 2726, 3926
PmaCI CACGTG 2 cut(s) 3193, 3223
PmlI CACGTG 2 cut(s) 3193, 3223
PpsI GAGTC 6 cut(s) 185, 688, 728, 772, 2726, 3926
Ppu21I YACGTR 3 cut(s) 1010, 3193, 3223
PscI ACATGT 4 cut(s) 3014, 3142, 3322, 3957
PshBI ATTAAT 1 cut(s) 1887
PsiI TTATAA 1 cut(s) 1064
Psp124BI GAGCTC 2 cut(s) 3726, 4027
Psp1406I AACGTT 1 cut(s) 3685
Psp6I CCWGG 3 cut(s) 201, 2315, 3837
PspCI CACGTG 2 cut(s) 3193, 3223
PspEI GGTNACC 2 cut(s) 2311, 3609
PspGI CCWGG 3 cut(s) 201, 2315, 3837
PspN4I GGNNCC 9 cut(s) 71, 178, 219, 399, 524, 909, 2353, 2876, 3458
PspPI GGNCC 3 cut(s) 70, 908, 3577
PstI CTGCAG 2 cut(s) 117, 2656
PstNI CAGNNNCTG 2 cut(s) 593, 3116
PsuI RGATCY 2 cut(s) 1310, 2278
RruI TCGCGA 1 cut(s) 2487
RseI CAYNNNNRTG 2 cut(s) 158, 3812
SacI GAGCTC 2 cut(s) 3726, 4027
SalI GTCGAC 2 cut(s) 418, 2762
SaqAI TTAA 9 cut(s) 1491, 1623, 1755, 1887, 2019, 2139, 2889, 3887, 4063
Sau96I GGNCC 3 cut(s) 70, 908, 3577
ScaI AGTACT 6 cut(s) 1524, 1656, 1788, 1920, 2052, 2172
SchI GAGTC 6 cut(s) 185, 689, 728, 773, 2726, 3926
ScrFI CCNGG 5 cut(s) 68, 203, 1957, 2317, 3839
SduI GDGCHC 6 cut(s) 287, 1044, 3726, 3965, 3982, 4027
SfcI CTRYAG 3 cut(s) 113, 2457, 2652
SinI GGWCC 3 cut(s) 70, 908, 3577
SmiMI CAYNNNNRTG 2 cut(s) 158, 3812
SmlI CTYRAG 4 cut(s) 125, 2572, 3736, 3971
SmoI CTYRAG 4 cut(s) 125, 2572, 3736, 3971
SpeI ACTAGT 3 cut(s) 1346, 1664, 2237
SphI GCATGC 1 cut(s) 296
SseBI AGGCCT 1 cut(s) 1050
SsiI CCGC 6 cut(s) 64, 1073, 1159, 1250, 3907, 4002
SspI AATATT 1 cut(s) 1779
SstI GAGCTC 2 cut(s) 3726, 4027
StuI AGGCCT 1 cut(s) 1050
StyD4I CCNGG 5 cut(s) 66, 201, 1955, 2315, 3837
StyI CCWWGG 3 cut(s) 429, 2347, 2757
TaaI ACNGT 7 cut(s) 651, 709, 1168, 2458, 2941, 3503, 3609
TaiI ACGT 6 cut(s) 419, 1012, 1237, 3195, 3225, 3688
TatI WGTACW 8 cut(s) 1522, 1654, 1786, 1918, 2050, 2170, 3145, 3942
TauI GCSGC 1 cut(s) 3909
Tru1I TTAA 9 cut(s) 1491, 1623, 1755, 1887, 2019, 2139, 2889, 3887, 4063
Tru9I TTAA 9 cut(s) 1491, 1623, 1755, 1887, 2019, 2139, 2889, 3887, 4063
TscAI CASTG 6 cut(s) 165, 1155, 1735, 2433, 2830, 3121
TseFI GTSAC 6 cut(s) 334, 1124, 2203, 2311, 3017, 3609
Tsp45I GTSAC 6 cut(s) 334, 1124, 2203, 2311, 3017, 3609
TspGWI ACGGA 3 cut(s) 987, 2814, 2857
TspRI CASTG 6 cut(s) 165, 1155, 1735, 2433, 2830, 3121
VneI GTGCAC 1 cut(s) 3961
VpaK11BI GGWCC 3 cut(s) 70, 908, 3577
VspI ATTAAT 1 cut(s) 1887
XapI RAATTY 4 cut(s) 507, 841, 1200, 1337
XceI RCATGY 6 cut(s) 296, 3018, 3146, 3326, 3811, 3961
XcmI CCANNNNNNNNNTGG 2 cut(s) 481, 2847
XmiI GTMKAC 4 cut(s) 419, 1437, 2763, 3804
XmnI GAANNNNTTC 3 cut(s) 1514, 1778, 2422
ZraI GACGTC 1 cut(s) 417
ZrmI AGTACT 6 cut(s) 1524, 1656, 1788, 1920, 2052, 2172
Zsp2I ATGCAT 1 cut(s) 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.