MD05G1243100.v1.1

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0011156
Physical Location & Seq
Reverse (-)
37585909 .. 37590583
4675 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1243100.v1.1.491

Sequence Viewer

Length: 3303 bp
ATGGCGTCATCACAAGTTGAAATGGCCTCCACCGCGCCTTTTGGGTGCGTGTTAAGGGATCACAACCGGCGAGACAGGTGCAGAGAAAGCAATGCCAGGGCCACGCAGGCTGCTTTTCAGAAGAATTTGAATAGCTTGGTGAGGAATCACCTCACCACCTGCATTTCTGTCTCATCAACCTCTGCCTCTAATGACCAGGAAAGCAACAACTCCCAAGACCAAAACATAGAGAATGTCGAAAGAGATGATGGTTGTTCGACAATGAGCCCGAGGCAGTCTCGGGTTCTTGATCGATGGGCTGAGAGACAAGGAGGGAAAGAAGCAATGGCAATGGTGGAGAGGCAGAGCCAGGAGGTAGAGCTTATGTCAGTGCCTAGTTCCGCGCCTTCATCAACGAGGACATCGACATCAATGAAGGAAGGGACTCTTTCAAGGACAGATAGCTTGGCGGATATTTCCAATCGGGGAGTTGGAGCTTCTTCGCTGGTTCAAATATGGGAGAAGAGGCTGAACCTATCAAACAGTTTCAAGATGAGTTCCAATGGCGGCACAGAGACGAGCAGGTCTAACTCTGGATTGAGCTTCAATGAGAATGAGAACATTTTTTATACTTTCAATGAATACAGTGCTTCCAATGAGTCTGTTGCTTCGAATCAACCTGTTTTCGAAAAGCAATTATCAAGAGAGTCTGAAGCAGGGGTCTCGGTTTGTGTTGATGAAAGGTATGACACTTGCCCGGTTGCTGAGGATTCCATTGAGGACACACATTCTGATGAAAGCGCCACAAGTGATGCCCGGAGTAATTTAGATGCAAGGGAAAGAGAAAGAGAAAGAGAGAAGGTTCGGGTTGTGGATATCATTAAACGGTTGACAATGACAGCACACTGTCCATTATCATCTCCAAGCGATGATAATGATCATGATCAGCAGTCCTGTGCCAATGCATCTCCGTCTAGAGAGCGCGATCGTGAGAGGTCTTTTGGGGTTGATCAGACAGAGCAGCTGCACAAAGGGTTCTCTCAGGTTATAAGTTCGCCGAGGATGATCAGAGGCAGGCAGGCATTTGCGGATTTGCTTATGCAATTGGAGCGTGACCGGCATAGGGAGCTTGACACACTTGTAGACCGCAGAGCAGTTTCAAGATTTTCACACAAAGGGCGAATTCAATCAATGTTACGGCTTAAACTGCTGCAACGCGGCATGGCAGGGGCAGCAGGTCAAGAAGTACCACGCCGGAAACCAGCAAAATCTCAAGTGAACAAGTTGCCACAAGGCTCTGCTGCCATCATGCATCTCAGGGAGAAATTCAGCGCTTGTTTCGAGCAGGCCTCAACAGCTCAGAGTGATGCAGCTAGCACAAAAATCCCTCAAAGGGAGGTCGTCAAGGACACCAGTGTACATGCACAAAACTCCTCCACCTCCAACATGCCTAGCGAAAACACTCCTAGTTGTAACATTGATATAACTGAGCAGAAGAATGCAACATGGGTGCAGACTCCATTGCCGTGTGCTAGTAATGATCTTCGTGAAGTAGTTAGCATGAGTTCTAAAGTCACTTGTCAAGAAACAAGCTCAGTGGCTACAAATACCTCAACGGATGCTAGTAAAGATCTTCGTGAGGAAGCTAGCCCAAGCTCAAAAGTCACCTCGGAAGGAACAAGTTCAGTGGCTACAAACAATTTGCTAGCTGCTAGTGAAGATCTTCGTGATGAAGCCAGCCTGAGTTTGAAGGTCACTTCTCAAGGATCGAGTCCAATGGCTAGGAACACCACGCCTCATGCTAGCGAAGATCTTCGTGAAGAAGCCAGCCCGAGTTTAAAAGTCACCTTGCAGGGAACAAGTCCAATGGCTAGGAGTACCTCGCCTCATGGTAGTGAAGATCTTCATGAAGAAGCGAGCCCAAGTTTAGAAGTCATCTTGCAAGGAACAAGTCCAATGGCTAGAAAAAACTCGCCTCATGCTAGTGAAGATCTTCACGAAGAAGCCAGCCCAAGCTCAAAAGTCACCTTGCAAGGAACAAGTTCAGAGGCCAGAAACAACTTGCCTCATACTAGTGAATATCTTCATGAAGAAGCTAGCCCGGGTTCAGAAGTTATCTGGCAAGGAACAAGTTCGGAGGCTAGAAACCTTGATGTGCAAGAAACCGCAGATGCAACCACATCCTTGGTTGGTTGGCATGAGAAGGAGATAGAAGAACAACTAGAGGATGATTACCAGTACTATGGAGATTATACTTATGACTGGATCAATGAGATTTCTCGCCCACGTAGCTACTGGGAAGACCTGAGGAAGGCATGGTACCAAGAGATGCTTAACTCCAACTCAGGTAAAGGAGAGATACGCCAACTTATCGAAAGAAGTACAGTATCAAATTTTCTTGCTAGTGATTTCCGAGACAGAATAGACAGGTTGATGGTATCTCGCTTAGAAATACAAGCGCAACCAGATGGCAGTCAAGAAGGAGAAGACAATGACATTAACCACGATAGGACAAATCCATTGGTGTCTTTCTTACAACAGCATCACCAAAATCAACAACAACAAGAATTGCAGCCGGCAGGCAGTCAAGAAGAACAACAAGTGCAAGAACAAAAACACCAAGAAGATCAGAACATGACAGAAGAAGATGATGATGAGGAAGAAGATGATGATGAGGAAGGTGATGACGATAAAGGAGGAGAAGAAAGGAGTCTAATTAGTGCTCAATATCAAGAAGCGAGTGATGATTTTTACCAGTGCACATCATTGCAGCCATCGCCATCTCATATGACATCGTGGAGCTATCAAGATATTGAAGTGGGAGATAATTTTGGTCGAGCTGCTTCTACATCTCCACCCCGACATTTTCCATCTCATTCTTACTATCCAAATAGTCGGCAAGGTTCACAATCACCATCTCTATCATACCATCAAAATCATCACCACGTCTCTTCCCCCTATCATCCTTCAATGGAAATGGAATTCATTTATGACATGAGAGGGCAGATGGAGAAACTCTATCGTGAGATGTCGGAGCTACGAAAAGTAATAAAGAGCTGTGTAGACATGCAACTGGTAATGCAGCAATCCATGAAGCAGGAAGTTCATTCAGGTCAAGCGGAGAGGAAGAGGTCGGCAAATGGATTACCAAAGAAAGGAAATTGTTGCATTTGCCATGAAGTGAAAGTCGACTCACTTTTTTACAGGTGTGGCCACATGTGCACCTGTCTCAAGTGTGCTCATGAGTTGCAATGGAACAGCGGAAAATGTCCAATCTGTCGGGCTCCAATCGTGGATGTCGTGAAGGCACAGATGGATTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

1101

Amino Acids

123.11

Weight (kDa)

5.11

Isoelectric Point (pI)

63.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 1045 - 1092 4.5e-12 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1028
AarI CACCTGC 1 cut(s) 167
AasI GACNNNNNNGTC 1 cut(s) 562
Acc36I ACCTGC 3 cut(s) 167, 552, 1205
Acc65I GGTACC 1 cut(s) 2298
AccB1I GGYRCC 1 cut(s) 2298
AccI GTMKAC 3 cut(s) 1122, 3042, 3168
AccII CGCG 4 cut(s) 35, 383, 963, 1197
AclWI GGATC 3 cut(s) 66, 1753, 2252
AcoI YGGCCR 1 cut(s) 3190
AcsI RAATTY 5 cut(s) 124, 1161, 1304, 2371, 2960
AcuI CTGAAG 1 cut(s) 711
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 1227, 1398, 1858, 2219, 2300, 2362
AfeI AGCGCT 1 cut(s) 1312
AfiI CCNNNNNNNGG 4 cut(s) 1102, 1372, 2290, 3134
AflIII ACRYGT 1 cut(s) 3195
AhlI ACTAGT 1 cut(s) 2051
AjiI CACGTC 1 cut(s) 2926
AjnI CCWGG 3 cut(s) 95, 195, 348
Alw21I GWGCWC 4 cut(s) 2704, 2741, 3203, 3220
Alw26I GTCTC 9 cut(s) 66, 175, 282, 298, 548, 706, 2388, 2932, 3212
Alw44I GTGCAC 2 cut(s) 2737, 3199
AlwI GGATC 3 cut(s) 66, 1753, 2252
Ama87I CYCGRG 4 cut(s) 268, 279, 1810, 2080
Aor51HI AGCGCT 1 cut(s) 1312
AoxI GGCC 5 cut(s) 24, 99, 1326, 2028, 3190
ApaLI GTGCAC 2 cut(s) 2737, 3199
ApoI RAATTY 5 cut(s) 124, 1161, 1304, 2371, 2960
Asp700I GAANNNNTTC 8 cut(s) 1660, 1701, 1791, 1881, 1971, 2020, 2061, 2110
Asp718I GGTACC 1 cut(s) 2298
AspLEI GCGC 6 cut(s) 37, 385, 782, 963, 1313, 2440
AspS9I GGNCC 1 cut(s) 99
AsuC2I CCSGG 4 cut(s) 737, 796, 2081, 2082
AsuII TTCGAA 2 cut(s) 650, 666
AsuNHI GCTAGC 5 cut(s) 1352, 1625, 1684, 1781, 2075
AvaI CYCGRG 4 cut(s) 268, 279, 1810, 2080
AxyI CCTNAGG 1 cut(s) 2285
BaeGI GKGCMC 2 cut(s) 2741, 3203
BalI TGGCCA 1 cut(s) 3192
BanI GGYRCC 1 cut(s) 2298
BanII GRGCYC 3 cut(s) 269, 1901, 3265
BarI GAAGNNNNNNTAC 2 cut(s) 2282, 2314
BbsI GAAGAC 2 cut(s) 2286, 2472
Bbv12I GWGCWC 4 cut(s) 2704, 2741, 3203, 3220
BbvCI CCTCAGC 1 cut(s) 744
BceAI ACGGC 2 cut(s) 1193, 1489
BcgI CGANNNNNNTGC 4 cut(s) 60, 94, 684, 718
BciT130I CCWGG 3 cut(s) 97, 197, 350
BclI TGATCA 4 cut(s) 916, 922, 988, 1044
BcnI CCSGG 4 cut(s) 737, 796, 2081, 2082
BcoDI GTCTC 9 cut(s) 66, 175, 282, 298, 548, 706, 2388, 2932, 3212
BcuI ACTAGT 1 cut(s) 2051
BfoI RGCGCY 2 cut(s) 783, 1314
BfuAI ACCTGC 3 cut(s) 167, 552, 1205
BglI GCCNNNNNGGC 1 cut(s) 107
BglII AGATCT 5 cut(s) 1609, 1699, 1789, 1879, 1969
BmcAI AGTACT 1 cut(s) 2219
Bme1390I CCNGG 7 cut(s) 97, 197, 350, 737, 796, 2081, 2082
BmeT110I CYCGRG 4 cut(s) 268, 279, 1810, 2080
BmgBI CACGTC 1 cut(s) 2926
BmgT120I GGNCC 1 cut(s) 99
BmiI GGNNCC 2 cut(s) 2300, 3264
BmrFI CCNGG 7 cut(s) 97, 197, 350, 737, 796, 2081, 2082
BmrI ACTGGG 1 cut(s) 2284
BmsI GCATC 9 cut(s) 781, 799, 953, 1300, 1336, 1588, 2140, 2298, 2530
BmtI GCTAGC 5 cut(s) 1356, 1629, 1688, 1785, 2079
BmuI ACTGGG 1 cut(s) 2284
BpiI GAAGAC 2 cut(s) 2286, 2472
BplI GAGNNNNNCTC 4 cut(s) 262, 294, 1313, 1345
Bpu10I CCTNAGC 1 cut(s) 744
Bpu14I TTCGAA 2 cut(s) 650, 666
BpuEI CTTGAG 3 cut(s) 1236, 1725, 3194
BpuMI CCSGG 4 cut(s) 737, 796, 2081, 2082
Bsa29I ATCGAT 1 cut(s) 292
BsaAI YACGTR 1 cut(s) 2267
BsaBI GATNNNNATC 2 cut(s) 915, 921
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 706
BsaJI CCNNGG 6 cut(s) 96, 269, 1037, 1647, 2080, 2163
Bsc4I CCNNNNNNNGG 4 cut(s) 1102, 1372, 2290, 3134
Bse118I RCCGGY 3 cut(s) 66, 1095, 2554
Bse1I ACTGG 6 cut(s) 1392, 2215, 2246, 2279, 2734, 3057
Bse21I CCTNAGG 1 cut(s) 2285
Bse3DI GCAATG 6 cut(s) 97, 330, 336, 1499, 2744, 3236
Bse8I GATNNNNATC 2 cut(s) 915, 921
BseBI CCWGG 3 cut(s) 97, 197, 350
BseCI ATCGAT 1 cut(s) 292
BseDI CCNNGG 6 cut(s) 96, 269, 1037, 1647, 2080, 2163
BseGI GGATG 6 cut(s) 1047, 1603, 2159, 2212, 2941, 3280
BseJI GATNNNNATC 2 cut(s) 915, 921
BseLI CCNNNNNNNGG 4 cut(s) 1102, 1372, 2290, 3134
BseMI GCAATG 6 cut(s) 97, 330, 336, 1499, 2744, 3236
BseNI ACTGG 6 cut(s) 1392, 2215, 2246, 2279, 2734, 3057
BseRI GAGGAG 2 cut(s) 1402, 2691
BseSI GKGCMC 2 cut(s) 2741, 3203
BsgI GTGCAG 3 cut(s) 100, 989, 1511
Bsh1236I CGCG 4 cut(s) 35, 383, 963, 1197
Bsh1285I CGRYCG 1 cut(s) 967
BshFI GGCC 5 cut(s) 26, 101, 1328, 2030, 3192
BshNI GGYRCC 1 cut(s) 2298
BshVI ATCGAT 1 cut(s) 292
BsiEI CGRYCG 1 cut(s) 967
BsiHKAI GWGCWC 4 cut(s) 2704, 2741, 3203, 3220
BsiHKCI CYCGRG 4 cut(s) 268, 279, 1810, 2080
BsiSI CCGG 7 cut(s) 67, 737, 796, 1096, 1234, 2081, 2555
BslFI GGGAC 1 cut(s) 436
BslI CCNNNNNNNGG 4 cut(s) 1102, 1372, 2290, 3134
BsmAI GTCTC 9 cut(s) 66, 175, 282, 298, 548, 706, 2388, 2932, 3212
BsmBI CGTCTC 2 cut(s) 548, 2932
BsmFI GGGAC 1 cut(s) 436
BsmI GAATGC 1 cut(s) 1483
BsnI GGCC 5 cut(s) 26, 101, 1328, 2030, 3192
Bso31I GGTCTC 1 cut(s) 706
BsoBI CYCGRG 4 cut(s) 268, 279, 1810, 2080
Bsp119I TTCGAA 2 cut(s) 650, 666
Bsp1286I GDGCHC 7 cut(s) 269, 1901, 2704, 2741, 3203, 3220, 3265
Bsp1407I TGTACA 1 cut(s) 1396
BspANI GGCC 5 cut(s) 26, 101, 1328, 2030, 3192
BspDI ATCGAT 1 cut(s) 292
BspFNI CGCG 4 cut(s) 35, 383, 963, 1197
BspHI TCATGA 4 cut(s) 919, 1885, 2065, 3220
BspLI GGNNCC 2 cut(s) 2300, 3264
BspMI ACCTGC 3 cut(s) 167, 552, 1205
BspOI GCTAGC 5 cut(s) 1356, 1629, 1688, 1785, 2079
BspPI GGATC 3 cut(s) 66, 1753, 2252
BspT104I TTCGAA 2 cut(s) 650, 666
BspT107I GGYRCC 1 cut(s) 2298
BspTNI GGTCTC 1 cut(s) 706
BsrDI GCAATG 6 cut(s) 97, 330, 336, 1499, 2744, 3236
BsrFI RCCGGY 3 cut(s) 66, 1095, 2554
BsrGI TGTACA 1 cut(s) 1396
BsrI ACTGG 6 cut(s) 1392, 2215, 2246, 2279, 2734, 3057
BssAI RCCGGY 3 cut(s) 66, 1095, 2554
BssECI CCNNGG 6 cut(s) 96, 269, 1037, 1647, 2080, 2163
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 2163
Bst2UI CCWGG 3 cut(s) 97, 197, 350
Bst4CI ACNGT 5 cut(s) 524, 626, 867, 887, 2365
Bst6I CTCTTC 3 cut(s) 497, 2935, 3101
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 1396
BstBAI YACGTR 1 cut(s) 2267
BstBI TTCGAA 2 cut(s) 650, 666
BstENI CCTNNNNNAGG 1 cut(s) 2288
BstF5I GGATG 6 cut(s) 1047, 1603, 2159, 2212, 2941, 3280
BstFNI CGCG 4 cut(s) 35, 383, 963, 1197
BstH2I RGCGCY 2 cut(s) 783, 1314
BstHHI GCGC 6 cut(s) 37, 385, 782, 963, 1313, 2440
BstMAI GTCTC 9 cut(s) 66, 175, 282, 298, 548, 706, 2388, 2932, 3212
BstMCI CGRYCG 1 cut(s) 967
BstNI CCWGG 3 cut(s) 97, 197, 350
BstNSI RCATGY 4 cut(s) 1403, 1429, 3049, 3199
BstSCI CCNGG 7 cut(s) 95, 195, 348, 735, 794, 2079, 2080
BstSLI GKGCMC 2 cut(s) 2741, 3203
BstUI CGCG 4 cut(s) 35, 383, 963, 1197
BstV2I GAAGAC 2 cut(s) 2286, 2472
BstX2I RGATCY 5 cut(s) 1609, 1699, 1789, 1879, 1969
BstXI CCANNNNNNTGG 2 cut(s) 2164, 2222
BstYI RGATCY 5 cut(s) 1609, 1699, 1789, 1879, 1969
Bsu15I ATCGAT 1 cut(s) 292
Bsu36I CCTNAGG 1 cut(s) 2285
BsuRI GGCC 5 cut(s) 26, 101, 1328, 2030, 3192
BsuTUI ATCGAT 1 cut(s) 292
BtgZI GCGATG 2 cut(s) 921, 2739
BtrI CACGTC 1 cut(s) 2926
BtsCI GGATG 6 cut(s) 1047, 1603, 2159, 2212, 2941, 3280
BtsIMutI CAGTG 7 cut(s) 375, 631, 883, 1399, 1581, 1671, 2741
BveI ACCTGC 3 cut(s) 167, 552, 1205
CciI TCATGA 4 cut(s) 919, 1885, 2065, 3220
CfoI GCGC 6 cut(s) 37, 385, 782, 963, 1313, 2440
Cfr10I RCCGGY 3 cut(s) 66, 1095, 2554
Cfr13I GGNCC 1 cut(s) 99
Cfr9I CCCGGG 1 cut(s) 2080
ClaI ATCGAT 1 cut(s) 292
Csp6I GTAC 6 cut(s) 1226, 1397, 1857, 2218, 2299, 2361
CspCI CAANNNNNGTGG 4 cut(s) 1557, 1592, 1647, 1682
CviQI GTAC 6 cut(s) 1226, 1397, 1857, 2218, 2299, 2361
DraI TTTAAA 1 cut(s) 1818
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 3190
Eam1104I CTCTTC 3 cut(s) 497, 2935, 3101
EarI CTCTTC 3 cut(s) 497, 2935, 3101
EciI GGCGGA 1 cut(s) 464
Eco130I CCWWGG 1 cut(s) 2163
Eco147I AGGCCT 1 cut(s) 1328
Eco24I GRGCYC 3 cut(s) 269, 1901, 3265
Eco31I GGTCTC 1 cut(s) 706
Eco32I GATATC 1 cut(s) 856
Eco47III AGCGCT 1 cut(s) 1312
Eco57I CTGAAG 1 cut(s) 711
Eco81I CCTNAGG 1 cut(s) 2285
Eco88I CYCGRG 4 cut(s) 268, 279, 1810, 2080
EcoNI CCTNNNNNAGG 1 cut(s) 2288
EcoRI GAATTC 2 cut(s) 1161, 2960
EcoRII CCWGG 3 cut(s) 95, 195, 348
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 2163
EcoT22I ATGCAT 2 cut(s) 946, 1293
EcoT38I GRGCYC 3 cut(s) 269, 1901, 3265
ErhI CCWWGG 1 cut(s) 2163
Esp3I CGTCTC 2 cut(s) 548, 2932
FalI AAGNNNNNCTT 4 cut(s) 411, 443, 2295, 2327
FaqI GGGAC 1 cut(s) 436
FauNDI CATATG 1 cut(s) 2766
FbaI TGATCA 4 cut(s) 916, 922, 988, 1044
FblI GTMKAC 3 cut(s) 1122, 3042, 3168
FokI GGATG 6 cut(s) 1054, 1610, 2146, 2219, 2928, 3287
FriOI GRGCYC 3 cut(s) 269, 1901, 3265
GlaI GCGC 6 cut(s) 36, 384, 781, 962, 1312, 2439
HaeII RGCGCY 2 cut(s) 783, 1314
HaeIII GGCC 5 cut(s) 26, 101, 1328, 2030, 3192
HapII CCGG 7 cut(s) 67, 737, 796, 1096, 1234, 2081, 2555
HhaI GCGC 6 cut(s) 37, 385, 782, 963, 1313, 2440
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 6 cut(s) 35, 383, 780, 961, 1311, 2438
HinP1I GCGC 6 cut(s) 35, 383, 780, 961, 1311, 2438
HincII GTYRAC 2 cut(s) 870, 3169
HindII GTYRAC 2 cut(s) 870, 3169
HpaII CCGG 7 cut(s) 67, 737, 796, 1096, 1234, 2081, 2555
Hpy166II GTNNAC 9 cut(s) 870, 1123, 1258, 1397, 2739, 2885, 3043, 3169, 3201
Hpy8I GTNNAC 9 cut(s) 870, 1123, 1258, 1397, 2739, 2885, 3043, 3169, 3201
HpyCH4III ACNGT 5 cut(s) 524, 626, 867, 887, 2365
HpyCH4IV ACGT 2 cut(s) 2266, 2925
HpySE526I ACGT 2 cut(s) 2266, 2925
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 6 cut(s) 35, 383, 780, 961, 1311, 2438
KpnI GGTACC 1 cut(s) 2302
KroI GCCGGC 1 cut(s) 2554
KroNI GCCGGC 1 cut(s) 2556
Ksp22I TGATCA 4 cut(s) 916, 922, 988, 1044
LmnI GCTCC 6 cut(s) 473, 1087, 1105, 2778, 3013, 3268
LweI GCATC 9 cut(s) 781, 799, 953, 1300, 1336, 1588, 2140, 2298, 2530
MaeII ACGT 2 cut(s) 2266, 2925
MaeIII GTNAC 8 cut(s) 1091, 1173, 1451, 1552, 1642, 1732, 1822, 2002
MfeI CAATTG 1 cut(s) 1082
MflI RGATCY 5 cut(s) 1609, 1699, 1789, 1879, 1969
MhlI GDGCHC 7 cut(s) 269, 1901, 2704, 2741, 3203, 3220, 3265
MlsI TGGCCA 1 cut(s) 3192
MluNI TGGCCA 1 cut(s) 3192
MlyI GAGTC 7 cut(s) 418, 647, 695, 1489, 1759, 2698, 3164
MmeI TCCRAC 4 cut(s) 451, 1446, 2343, 2991
Mox20I TGGCCA 1 cut(s) 3192
Mph1103I ATGCAT 2 cut(s) 946, 1293
MroNI GCCGGC 1 cut(s) 2554
MroXI GAANNNNTTC 8 cut(s) 1660, 1701, 1791, 1881, 1971, 2020, 2061, 2110
MscI TGGCCA 1 cut(s) 3192
MseI TTAA 6 cut(s) 53, 861, 1182, 1817, 2313, 2478
MslI CAYNNNNRTG 5 cut(s) 771, 1504, 1872, 1962, 2052
Msp20I TGGCCA 1 cut(s) 3192
MspA1I CMGCKG 2 cut(s) 1003, 3240
MspI CCGG 7 cut(s) 67, 737, 796, 1096, 1234, 2081, 2555
MspR9I CCNGG 7 cut(s) 97, 197, 350, 737, 796, 2081, 2082
MunI CAATTG 1 cut(s) 1082
Mva1269I GAATGC 1 cut(s) 1483
MvaI CCWGG 3 cut(s) 97, 197, 350
MvnI CGCG 4 cut(s) 35, 383, 963, 1197
NaeI GCCGGC 1 cut(s) 2556
NciI CCSGG 4 cut(s) 737, 796, 2081, 2082
NdeI CATATG 1 cut(s) 2766
NgoMIV GCCGGC 1 cut(s) 2554
NheI GCTAGC 5 cut(s) 1352, 1625, 1684, 1781, 2075
NlaIV GGNNCC 2 cut(s) 2300, 3264
NmeAIII GCCGAG 1 cut(s) 1062
NmuCI GTSAC 6 cut(s) 1091, 1552, 1642, 1732, 1822, 2002
NsiI ATGCAT 2 cut(s) 946, 1293
NspI RCATGY 4 cut(s) 1403, 1429, 3049, 3199
NspV TTCGAA 2 cut(s) 650, 666
PagI TCATGA 4 cut(s) 919, 1885, 2065, 3220
PaqCI CACCTGC 1 cut(s) 167
PceI AGGCCT 1 cut(s) 1328
PciI ACATGT 1 cut(s) 3195
PcsI WCGNNNNNNNCGW 2 cut(s) 401, 3276
PctI GAATGC 1 cut(s) 1483
PdiI GCCGGC 1 cut(s) 2556
PdmI GAANNNNTTC 8 cut(s) 1660, 1701, 1791, 1881, 1971, 2020, 2061, 2110
PfeI GAWTC 3 cut(s) 145, 652, 749
Ple19I CGATCG 1 cut(s) 967
PleI GAGTC 7 cut(s) 418, 646, 694, 1489, 1758, 2697, 3164
PpsI GAGTC 7 cut(s) 418, 646, 694, 1489, 1758, 2697, 3164
Ppu21I YACGTR 1 cut(s) 2267
PscI ACATGT 1 cut(s) 3195
PsiI TTATAA 1 cut(s) 1028
Psp6I CCWGG 3 cut(s) 95, 195, 348
PspGI CCWGG 3 cut(s) 95, 195, 348
PspN4I GGNNCC 2 cut(s) 2300, 3264
PspPI GGNCC 1 cut(s) 99
PsuI RGATCY 5 cut(s) 1609, 1699, 1789, 1879, 1969
PvuI CGATCG 1 cut(s) 967
PvuII CAGCTG 1 cut(s) 1003
RsaI GTAC 6 cut(s) 1227, 1398, 1858, 2219, 2300, 2362
RsaNI GTAC 6 cut(s) 1226, 1397, 1857, 2218, 2299, 2361
RseI CAYNNNNRTG 5 cut(s) 771, 1504, 1872, 1962, 2052
SalI GTCGAC 1 cut(s) 3167
SaqAI TTAA 6 cut(s) 53, 861, 1182, 1817, 2313, 2478
Sau96I GGNCC 1 cut(s) 99
ScaI AGTACT 1 cut(s) 2219
SchI GAGTC 7 cut(s) 418, 647, 695, 1489, 1759, 2698, 3164
ScrFI CCNGG 7 cut(s) 97, 197, 350, 737, 796, 2081, 2082
SduI GDGCHC 7 cut(s) 269, 1901, 2704, 2741, 3203, 3220, 3265
SfaNI GCATC 9 cut(s) 781, 799, 953, 1300, 1336, 1588, 2140, 2298, 2530
SfuI TTCGAA 2 cut(s) 650, 666
SmaI CCCGGG 1 cut(s) 2082
SmiMI CAYNNNNRTG 5 cut(s) 771, 1504, 1872, 1962, 2052
SmlI CTYRAG 3 cut(s) 1251, 1740, 3209
SmoI CTYRAG 3 cut(s) 1251, 1740, 3209
SpeI ACTAGT 1 cut(s) 2051
SseBI AGGCCT 1 cut(s) 1328
StuI AGGCCT 1 cut(s) 1328
StyD4I CCNGG 7 cut(s) 95, 195, 348, 735, 794, 2079, 2080
StyI CCWWGG 1 cut(s) 2163
TaaI ACNGT 5 cut(s) 524, 626, 867, 887, 2365
TaiI ACGT 2 cut(s) 2269, 2928
TatI WGTACW 3 cut(s) 1396, 2217, 2360
TauI GCSGC 2 cut(s) 549, 1200
TfiI GAWTC 3 cut(s) 145, 652, 749
Tru1I TTAA 6 cut(s) 53, 861, 1182, 1817, 2313, 2478
Tru9I TTAA 6 cut(s) 53, 861, 1182, 1817, 2313, 2478
TscAI CASTG 7 cut(s) 375, 631, 890, 1399, 1581, 1671, 2741
TseFI GTSAC 6 cut(s) 1091, 1552, 1642, 1732, 1822, 2002
Tsp45I GTSAC 6 cut(s) 1091, 1552, 1642, 1732, 1822, 2002
TspGWI ACGGA 2 cut(s) 939, 1610
TspMI CCCGGG 1 cut(s) 2080
TspRI CASTG 7 cut(s) 375, 631, 890, 1399, 1581, 1671, 2741
VneI GTGCAC 2 cut(s) 2737, 3199
XagI CCTNNNNNAGG 1 cut(s) 2288
XapI RAATTY 5 cut(s) 124, 1161, 1304, 2371, 2960
XbaI TCTAGA 1 cut(s) 953
XceI RCATGY 4 cut(s) 1403, 1429, 3049, 3199
XcmI CCANNNNNNNNNTGG 1 cut(s) 2271
XmaI CCCGGG 1 cut(s) 2080
XmiI GTMKAC 3 cut(s) 1122, 3042, 3168
XmnI GAANNNNTTC 8 cut(s) 1660, 1701, 1791, 1881, 1971, 2020, 2061, 2110
ZrmI AGTACT 1 cut(s) 2219
Zsp2I ATGCAT 2 cut(s) 946, 1293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.