Rorug05G0069000

GPI-anchored adhesin-like protein PGA55

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0011156
Physical Location & Seq
Reverse (-)
5919734 .. 5920682
949 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0069000.1

Sequence Viewer

Length: 744 bp
ATGGCAGAGCCATATATTACTCTACCGGAGTTGGTCACAGCACTTCAACTTCACCCAGCTAAAACTGGCCATGTGCACCGGCTCATGCGCCTAATGGTACACTCTGGTTTCTTTGCTACAAAACAAGTCTGTATGAATCAAGATGAACAAGAGGAAGATGCATATGATCTTACACCGTCTTCTAGGCTCCTCCTAAAAGACAAGGTTCCGAGCATGTTACCCATTGTTACAGCAATGCTTGATCCAGCTTTTACAACCCCATGGCAGTTTTTGGGAAAATGGTTCCGTGGGAATGAGCTCACACCGTTTGAGACTGCACATGGGATGGGATTGTGGGAATACGGAGATAAAAATCCTGAGTTCAACAGTCTTTTTAATGAAACAATGGCCAGTGATTCCAGAATGATGAACCTGGTCATCAAAGACTGCAAGCCAATCTCTGAGGGGTTGAGTTCTTTAGTTGATGTAGGAGGCGGTACAGGAACAGTTGCTAGGATCCTTTGTGAGGCTTTCCCGAACTTGAAATGCACAGTTCTTGAACTTCCCCAAGTTGTTGCTAACTTGCCGGATAGTGCGAATTTGAAGTTCATTGCAGGTGATATGTTCAATGCTATTCCTCCAGCTGATGCTATTCTGCTCAAGTTAACTTTACATGATTTGAGCGACGAGGAATGCTTGAAGGTTCTGAGGAAATGTAGGGAAGCTATTCCAGACAGTGGTCAAGGAAAGGTTATTATCATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000209 GO:0001654 GO:0001709 GO:0002009 GO:0002064 GO:0002065 GO:0002066 GO:0002165 GO:0002682 GO:0002683 GO:0003002 GO:0003006 GO:0003008 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0006464 GO:0006807 GO:0007275 GO:0007276 GO:0007281 GO:0007292 GO:0007389 GO:0007398 GO:0007399 GO:0007422 GO:0007423 GO:0007444 GO:0007472 GO:0007476 GO:0007498 GO:0007552 GO:0007560 GO:0007610 GO:0007611 GO:0007613 GO:0007616 GO:0008052 GO:0008104 GO:0008150 GO:0008152 GO:0008270 GO:0008289 GO:0008356 GO:0008593 GO:0009653 GO:0009791 GO:0009886 GO:0009887 GO:0009888 GO:0009966 GO:0009967 GO:0009987 GO:0010160 GO:0010646 GO:0010647 GO:0016020 GO:0016043 GO:0016360 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019827 GO:0019953 GO:0022008 GO:0022412 GO:0022414 GO:0022603 GO:0022607 GO:0023051 GO:0023056 GO:0030154 GO:0030707 GO:0030718 GO:0030855 GO:0032446 GO:0032501 GO:0032502 GO:0032504 GO:0033036 GO:0035091 GO:0035107 GO:0035114 GO:0035120 GO:0035203 GO:0035204 GO:0035220 GO:0035239 GO:0035295 GO:0036211 GO:0042478 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043412 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044703 GO:0045165 GO:0045314 GO:0045595 GO:0045596 GO:0045610 GO:0045611 GO:0045613 GO:0045614 GO:0045664 GO:0045747 GO:0046532 GO:0046872 GO:0046914 GO:0048468 GO:0048471 GO:0048477 GO:0048513 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048563 GO:0048569 GO:0048580 GO:0048581 GO:0048583 GO:0048584 GO:0048609 GO:0048645 GO:0048646 GO:0048699 GO:0048707 GO:0048729 GO:0048731 GO:0048736 GO:0048737 GO:0048749 GO:0048856 GO:0048859 GO:0048869 GO:0050767 GO:0050789 GO:0050793 GO:0050794 GO:0050877 GO:0050890 GO:0051093 GO:0051179 GO:0051239 GO:0051241 GO:0051259 GO:0051260 GO:0051301 GO:0051704 GO:0051960 GO:0060284 GO:0060429 GO:0060562 GO:0060581 GO:0060582 GO:0061630 GO:0061659 GO:0065003 GO:0065007 GO:0070647 GO:0071704 GO:0071840 GO:0071944 GO:0098727 GO:0140096 GO:1901564 GO:1901981 GO:1903706 GO:1903707 GO:2000026 GO:2000027
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

247

Amino Acids

27.34

Weight (kDa)

5.15

Isoelectric Point (pI)

35.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dimerisation PF08100 5 - 67 9.1e-08 O-methyltransferase dimerisation domain
Methyltransf_2 PF00891 88 - 247 1.3e-39 O-methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G34920
fragaria_vesca FvH4_3g13290 FvH4_3g13290
malus_domestica MD05G1243100.v1.1 MD10G1225200.v1.1
prunus_persica Prupe.4G120300_v2.0.a1 Prupe.4G120300_v2.0.a1
pyrus_communis pycom10g18910
rosa_chinensis RchiOBHm_Chr5g0021761
rosa_laevigata RLG00000032625
rosa_multiflora Rmu_ssc0000233.1_g000006
rosa_roxburghii Rroxscaffold_1G00056950
rosa_rugosa Rorug05G0069000 Rorug05G0069100 Rorug05G0069200
rosa_samantha Rh5AG157700 Rh5BG157300 Rh5CG171500 Rh5DG157600
rosa_wichuraiana Rw5G014050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 584
Acc36I ACCTGC 1 cut(s) 584
AccB7I CCANNNNNTGG 1 cut(s) 716
AciI CCGC 1 cut(s) 474
AclWI GGATC 3 cut(s) 236, 490, 503
AcoI YGGCCR 2 cut(s) 67, 387
AcsI RAATTY 1 cut(s) 577
AfaI GTAC 2 cut(s) 99, 478
AfiI CCNNNNNNNGG 2 cut(s) 505, 716
AgsI TTSAA 7 cut(s) 47, 364, 523, 539, 583, 607, 679
AjnI CCWGG 1 cut(s) 411
AluBI AGCT 5 cut(s) 59, 248, 298, 623, 704
AluI AGCT 5 cut(s) 59, 248, 298, 623, 704
Alw21I GWGCWC 2 cut(s) 78, 300
Alw26I GTCTC 1 cut(s) 305
Alw44I GTGCAC 1 cut(s) 74
AlwI GGATC 3 cut(s) 236, 490, 503
AoxI GGCC 2 cut(s) 67, 387
ApaLI GTGCAC 1 cut(s) 74
ApoI RAATTY 1 cut(s) 577
Asp700I GAANNNNTTC 1 cut(s) 705
AspLEI GCGC 1 cut(s) 90
AsuHPI GGTGA 2 cut(s) 44, 608
BaeGI GKGCMC 1 cut(s) 78
BalI TGGCCA 2 cut(s) 69, 389
BamHI GGATCC 1 cut(s) 495
BanII GRGCYC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 78, 300
BccI CCATC 1 cut(s) 319
BciT130I CCWGG 1 cut(s) 413
BcoDI GTCTC 1 cut(s) 305
BfaI CTAG 2 cut(s) 183, 492
BfuAI ACCTGC 1 cut(s) 584
Bme1390I CCNGG 1 cut(s) 413
BmiI GGNNCC 4 cut(s) 188, 207, 284, 497
BmrFI CCNGG 1 cut(s) 413
BmsI GCATC 2 cut(s) 148, 616
BoxI GACNNNNGTC 1 cut(s) 717
BpiI GAAGAC 1 cut(s) 171
BpmI CTGGAG 1 cut(s) 603
BpuEI CTTGAG 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 260, 286
BsaWI WCCGGW 1 cut(s) 25
Bsc4I CCNNNNNNNGG 2 cut(s) 505, 716
Bse118I RCCGGY 1 cut(s) 78
Bse1I ACTGG 2 cut(s) 70, 390
Bse3DI GCAATG 2 cut(s) 240, 588
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 1 cut(s) 413
BseDI CCNNGG 2 cut(s) 260, 286
BseGI GGATG 1 cut(s) 330
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 2 cut(s) 505, 716
BseMI GCAATG 2 cut(s) 240, 588
BseMII CTCAG 3 cut(s) 348, 432, 677
BseNI ACTGG 2 cut(s) 70, 390
BseRI GAGGAG 1 cut(s) 179
BseSI GKGCMC 1 cut(s) 78
BseYI CCCAGC 1 cut(s) 55
BsgI GTGCAG 1 cut(s) 300
BshFI GGCC 2 cut(s) 69, 389
BsiHKAI GWGCWC 2 cut(s) 78, 300
BsiSI CCGG 3 cut(s) 26, 79, 566
BslI CCNNNNNNNGG 2 cut(s) 505, 716
BsmAI GTCTC 1 cut(s) 305
BsmI GAATGC 1 cut(s) 677
BsnI GGCC 2 cut(s) 69, 389
Bsp1286I GDGCHC 2 cut(s) 78, 300
Bsp143I GATC 3 cut(s) 166, 241, 495
Bsp19I CCATGG 1 cut(s) 260
BspACI CCGC 1 cut(s) 474
BspANI GGCC 2 cut(s) 69, 389
BspCNI CTCAG 3 cut(s) 349, 433, 678
BspLI GGNNCC 4 cut(s) 188, 207, 284, 497
BspMI ACCTGC 1 cut(s) 584
BspPI GGATC 3 cut(s) 236, 490, 503
BsrDI GCAATG 2 cut(s) 240, 588
BsrFI RCCGGY 1 cut(s) 78
BsrI ACTGG 2 cut(s) 70, 390
BssAI RCCGGY 1 cut(s) 78
BssECI CCNNGG 2 cut(s) 260, 286
BssMI GATC 3 cut(s) 166, 241, 495
BssT1I CCWWGG 1 cut(s) 260
Bst2UI CCWGG 1 cut(s) 413
Bst4CI ACNGT 6 cut(s) 177, 306, 368, 487, 532, 716
BstC8I GCNNGC 1 cut(s) 431
BstDEI CTNAG 3 cut(s) 357, 441, 686
BstDSI CCRYGG 2 cut(s) 260, 286
BstENI CCTNNNNNAGG 1 cut(s) 503
BstF5I GGATG 1 cut(s) 330
BstHHI GCGC 1 cut(s) 90
BstKTI GATC 3 cut(s) 169, 244, 498
BstMAI GTCTC 1 cut(s) 305
BstMBI GATC 3 cut(s) 166, 241, 495
BstNI CCWGG 1 cut(s) 413
BstNSI RCATGY 1 cut(s) 217
BstPAI GACNNNNGTC 1 cut(s) 717
BstSCI CCNGG 1 cut(s) 411
BstSLI GKGCMC 1 cut(s) 78
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 495
BstYI RGATCY 1 cut(s) 495
BsuRI GGCC 2 cut(s) 69, 389
BtgI CCRYGG 2 cut(s) 260, 286
BtsCI GGATG 1 cut(s) 330
BtsIMutI CAGTG 2 cut(s) 397, 721
BveI ACCTGC 1 cut(s) 584
Cac8I GCNNGC 1 cut(s) 431
CfoI GCGC 1 cut(s) 90
Cfr10I RCCGGY 1 cut(s) 78
CsiI ACCWGGT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 98, 477
CviAII CATG 6 cut(s) 71, 85, 214, 261, 320, 653
CviQI GTAC 2 cut(s) 98, 477
DdeI CTNAG 3 cut(s) 357, 441, 686
DpnI GATC 3 cut(s) 168, 243, 497
DpnII GATC 3 cut(s) 166, 241, 495
EaeI YGGCCR 2 cut(s) 67, 387
Ecl136II GAGCTC 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 260
Eco24I GRGCYC 1 cut(s) 300
Eco53kI GAGCTC 1 cut(s) 298
EcoICRI GAGCTC 1 cut(s) 298
EcoNI CCTNNNNNAGG 1 cut(s) 503
EcoRII CCWGG 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 260
EcoT22I ATGCAT 1 cut(s) 163
EcoT38I GRGCYC 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 260
FaeI CATG 6 cut(s) 74, 88, 217, 264, 323, 656
FatI CATG 6 cut(s) 70, 84, 213, 260, 319, 652
FauNDI CATATG 1 cut(s) 163
FokI GGATG 1 cut(s) 337
FriOI GRGCYC 1 cut(s) 300
FspBI CTAG 2 cut(s) 183, 492
GlaI GCGC 1 cut(s) 89
GsaI CCCAGC 1 cut(s) 59
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 2 cut(s) 69, 389
HapII CCGG 3 cut(s) 26, 79, 566
HhaI GCGC 1 cut(s) 90
Hin1II CATG 6 cut(s) 74, 88, 217, 264, 323, 656
Hin6I GCGC 1 cut(s) 88
HinP1I GCGC 1 cut(s) 88
HincII GTYRAC 1 cut(s) 645
HindII GTYRAC 1 cut(s) 645
HinfI GANTC 2 cut(s) 136, 395
HpaI GTTAAC 1 cut(s) 645
HpaII CCGG 3 cut(s) 26, 79, 566
HphI GGTGA 2 cut(s) 44, 608
Hpy166II GTNNAC 3 cut(s) 76, 100, 645
Hpy188I TCNGA 3 cut(s) 210, 442, 687
Hpy188III TCNNGA 6 cut(s) 140, 356, 399, 514, 536, 710
Hpy8I GTNNAC 3 cut(s) 76, 100, 645
Hpy99I CGWCG 1 cut(s) 668
HpyAV CCTTC 1 cut(s) 673
HpyCH4III ACNGT 6 cut(s) 177, 306, 368, 487, 532, 716
HpyCH4V TGCA 6 cut(s) 76, 161, 317, 429, 528, 593
HpyF3I CTNAG 3 cut(s) 357, 441, 686
Hsp92II CATG 6 cut(s) 74, 88, 217, 264, 323, 656
HspAI GCGC 1 cut(s) 88
KspAI GTTAAC 1 cut(s) 645
Kzo9I GATC 3 cut(s) 166, 241, 495
LmnI GCTCC 1 cut(s) 192
LweI GCATC 2 cut(s) 148, 616
MabI ACCWGGT 1 cut(s) 411
MaeI CTAG 2 cut(s) 183, 492
MaeIII GTNAC 3 cut(s) 34, 216, 226
MalI GATC 3 cut(s) 168, 243, 497
MboI GATC 3 cut(s) 166, 241, 495
MboII GAAGA 2 cut(s) 167, 171
MflI RGATCY 1 cut(s) 495
MhlI GDGCHC 2 cut(s) 78, 300
MlsI TGGCCA 2 cut(s) 69, 389
MluCI AATT 1 cut(s) 577
MluNI TGGCCA 2 cut(s) 69, 389
MnlI CCTC 8 cut(s) 145, 200, 436, 464, 499, 627, 661, 681
Mox20I TGGCCA 2 cut(s) 69, 389
Mph1103I ATGCAT 1 cut(s) 163
MroXI GAANNNNTTC 1 cut(s) 705
MscI TGGCCA 2 cut(s) 69, 389
MseI TTAA 2 cut(s) 375, 644
Msp20I TGGCCA 2 cut(s) 69, 389
MspA1I CMGCKG 1 cut(s) 623
MspI CCGG 3 cut(s) 26, 79, 566
MspR9I CCNGG 1 cut(s) 413
Mva1269I GAATGC 1 cut(s) 677
MvaI CCWGG 1 cut(s) 413
NcoI CCATGG 1 cut(s) 260
NdeI CATATG 1 cut(s) 163
NdeII GATC 3 cut(s) 166, 241, 495
NlaIII CATG 6 cut(s) 74, 88, 217, 264, 323, 656
NlaIV GGNNCC 4 cut(s) 188, 207, 284, 497
NmuCI GTSAC 1 cut(s) 34
NsiI ATGCAT 1 cut(s) 163
NspI RCATGY 1 cut(s) 217
PaqCI CACCTGC 1 cut(s) 584
PctI GAATGC 1 cut(s) 677
PdmI GAANNNNTTC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 136, 395
PflMI CCANNNNNTGG 1 cut(s) 716
PshAI GACNNNNGTC 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 411
PspFI CCCAGC 1 cut(s) 55
PspGI CCWGG 1 cut(s) 411
PspN4I GGNNCC 4 cut(s) 188, 207, 284, 497
PsuI RGATCY 1 cut(s) 495
PvuII CAGCTG 1 cut(s) 623
RsaI GTAC 2 cut(s) 99, 478
RsaNI GTAC 2 cut(s) 98, 477
SacI GAGCTC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 375, 644
Sau3AI GATC 3 cut(s) 166, 241, 495
ScrFI CCNGG 1 cut(s) 413
SduI GDGCHC 2 cut(s) 78, 300
SexAI ACCWGGT 1 cut(s) 411
SfaNI GCATC 2 cut(s) 148, 616
SmlI CTYRAG 1 cut(s) 638
SmoI CTYRAG 1 cut(s) 638
Sse9I AATT 1 cut(s) 577
SsiI CCGC 1 cut(s) 474
SspMI CTAG 2 cut(s) 183, 492
SstI GAGCTC 1 cut(s) 300
StyD4I CCNGG 1 cut(s) 411
StyI CCWWGG 1 cut(s) 260
TaaI ACNGT 6 cut(s) 177, 306, 368, 487, 532, 716
TasI AATT 1 cut(s) 577
TfiI GAWTC 2 cut(s) 136, 395
Tru1I TTAA 2 cut(s) 375, 644
Tru9I TTAA 2 cut(s) 375, 644
TscAI CASTG 2 cut(s) 397, 721
TseFI GTSAC 1 cut(s) 34
Tsp45I GTSAC 1 cut(s) 34
TspDTI ATGAA 5 cut(s) 149, 159, 393, 422, 577
TspGWI ACGGA 2 cut(s) 275, 357
TspRI CASTG 2 cut(s) 397, 721
Van91I CCANNNNNTGG 1 cut(s) 716
VneI GTGCAC 1 cut(s) 74
XagI CCTNNNNNAGG 1 cut(s) 503
XapI RAATTY 1 cut(s) 577
XceI RCATGY 1 cut(s) 217
XmnI GAANNNNTTC 1 cut(s) 705
XspI CTAG 2 cut(s) 183, 492
Zsp2I ATGCAT 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.