FvH4_3g19420

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009552
Physical Location & Seq
Forward (+)
12590227 .. 12597725
7499 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g19420.t1

Sequence Viewer

Length: 1596 bp
ATGGCGGAAGAATACTCAGTCGACGATCCAACTCAGCTTCTCGAAGCTTCTTCCGAGTTCGCAAACTATCCCGGAGTTCAAAATGACGCTTCCGCCCACGAGTTTCTGGACCGCTTCCCCCTCCCTGTCATCCTAAATGCTTTGCAGACGAAAGCCGATGTGCCTGGTTTAGAAAACACCTTGGTTGATGTTTTGGAGAGCATTTTCAAAACAAGATATGGTACTTCGCTCATTCCACAGTATATGCCTTTCATACAAGTTGGTCTGACGGCAAATTCTCAGAAGGTCAAAGCTTTAGCCTGTAAAACGTCTGCCATCTTAACTGGCATTCAGGTTACTCGCCTTCTCGAAAGTGTGAATGACTATGCTGTTTCTGCACATCTTATAATCGACAACAATATATATCCTCTATTGATTGATTGCCTCATCAATGGAAATGAACAAGTTACAACTTTAGCAACTGAAGCGATAGCAAAGATAGCGGGTAGTCCAGCAGGCATAGATATTGTTTTCCCAGCTGATGCCAATGAAGCTACACATCTTGGAAAATTAGCATCTCAATGCTCATCACTGGGACGGGTCCGAATTTTGGCTTTGATTGTGAAGCTATTCTCTGTCTCCCATCATGTGGCATCGGTGGTTTCAAAGTCAAATCTACTTACACTTTTCGAAGCGGAGATTAACTCGAATGATACCCTTGCAACCTTAAATGTTATGGAGCTTTTGTATGAGTTGTCAGAGATCAAGCATGGTAGAGTTTTCTTGTCAACAAGCACCCTCCTGCAGCTGCTTAGTTCTATAATAAGCAACCAGTCAATGGACTCGATTCTAAGATCAAGGGCGATGATAATCAGCGGAAGACTGTTGTCGAATGGAAATTACATTTTGTCTGATGAATCTAGTGCGAAGACTGTAGTATCAGCTATAGATAGAATACTTAGTTCATCAGAAAGTCATGATACAGATGAATGTGAAACTGCTCTTGAAGCCCTGGGTCAAATAGGGTCATCAATGGAAGGAGCACAATTGCTACTGTCAGGCTCACCACCAGCTGCAAGACATGTTATATATTTTGCCTTTGATAGGCAGGGCCATGGTAGAAAATTGGCTGCTTTGCATGCACTTGCAAACATTTCTGGAGAAACTCGATCTGAGAACAGCGTCATACTGACTAATGATGCTGAAGAAAGCCTACGACGCTTGATTTATGAAACTGCATCCAAGAGCTCAAAGCTGACACCATCAGGTCTATTCCTATCAGTTATCCAACAGGATCCAGAGACTCGTTTGGCGGGTTATAGAGTGCTGACTGGGTTGGGGGCTCGCCCATGGTGCCTCACGGAGATTTGCTCAAAGCAAGAGATAATAAATGTAGTGACTGATGCTGCTTCTGAAACCACAAAACTAGGCATGGAAGCAAGATATAATTGTTGCAAGACTATCCATACGGCTTTTAACATGTCCAGTAAACTTTCAAGCGACCCAGCGCTTGGTGGGATTGCAGCAAAGTTGCATGAATCTGTGAGACTGGGTCCCTATCTTGCCAGAAAGCATCGTGAAGCTCAACCTACAGTAGTTACAGCCGACAGATTCTAG

Protein Analysis

532

Amino Acids

57.39

Weight (kDa)

5.52

Isoelectric Point (pI)

37.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasom_PSMB PF10508 61 - 519 4.9e-21 Proteasome non-ATPase 26S subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 386
AccB1I GGYRCC 1 cut(s) 1332
AccB7I CCANNNNNTGG 3 cut(s) 628, 817, 1490
AccI GTMKAC 1 cut(s) 21
AciI CCGC 7 cut(s) 5, 93, 112, 482, 674, 855, 1292
AclWI GGATC 3 cut(s) 20, 1268, 1281
AcsI RAATTY 2 cut(s) 274, 585
AcuI CTGAAG 2 cut(s) 483, 1203
AfaI GTAC 1 cut(s) 223
AfeI AGCGCT 1 cut(s) 1488
AfiI CCNNNNNNNGG 5 cut(s) 589, 628, 817, 1083, 1490
AflIII ACRYGT 2 cut(s) 1060, 1458
AgsI TTSAA 5 cut(s) 80, 208, 645, 986, 1476
AjnI CCWGG 2 cut(s) 163, 990
Alw21I GWGCWC 2 cut(s) 1024, 1229
Alw26I GTCTC 3 cut(s) 622, 1274, 1519
AlwI GGATC 3 cut(s) 20, 1268, 1281
Aor51HI AGCGCT 1 cut(s) 1488
AoxI GGCC 1 cut(s) 1090
ApeKI GCWGC 6 cut(s) 784, 787, 1052, 1109, 1385, 1502
ApoI RAATTY 2 cut(s) 274, 585
Asp700I GAANNNNTTC 1 cut(s) 608
AspLEI GCGC 1 cut(s) 1489
AspS9I GGNCC 4 cut(s) 109, 580, 1090, 1532
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 1 cut(s) 1035
AsuII TTCGAA 1 cut(s) 669
AvaII GGWCC 3 cut(s) 109, 580, 1532
BaeI ACNNNNGTAYC 2 cut(s) 900, 933
BamHI GGATCC 1 cut(s) 1273
BanI GGYRCC 1 cut(s) 1332
BanII GRGCYC 2 cut(s) 1229, 1324
BarI GAAGNNNNNNTAC 2 cut(s) 1176, 1208
BauI CACGAG 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 865, 914
Bbv12I GWGCWC 2 cut(s) 1024, 1229
BbvI GCAGC 6 cut(s) 774, 796, 1039, 1096, 1372, 1514
BccI CCATC 3 cut(s) 323, 630, 1249
BceAI ACGGC 2 cut(s) 285, 1464
BciT130I CCWGG 2 cut(s) 165, 992
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 3 cut(s) 622, 1274, 1519
BfaI CTAG 3 cut(s) 900, 1406, 1594
BfmI CTRYAG 4 cut(s) 782, 912, 924, 1569
BfoI RGCGCY 1 cut(s) 1490
BisI GCNGC 6 cut(s) 785, 788, 1053, 1110, 1386, 1503
BlsI GCNGC 6 cut(s) 786, 789, 1054, 1111, 1387, 1504
Bme1390I CCNGG 3 cut(s) 72, 165, 992
Bme18I GGWCC 3 cut(s) 109, 580, 1532
BmgT120I GGNCC 4 cut(s) 109, 580, 1090, 1532
BmiI GGNNCC 5 cut(s) 581, 1275, 1334, 1533, 1534
BmrFI CCNGG 3 cut(s) 72, 165, 992
BmrI ACTGGG 3 cut(s) 581, 1320, 1538
BmsI GCATC 7 cut(s) 511, 563, 641, 1168, 1226, 1372, 1561
BmuI ACTGGG 3 cut(s) 581, 1320, 1538
BoxI GACNNNNGTC 1 cut(s) 865
BpiI GAAGAC 2 cut(s) 865, 914
BplI GAGNNNNNCTC 4 cut(s) 668, 700, 1334, 1366
BpmI CTGGAG 1 cut(s) 1158
Bpu14I TTCGAA 1 cut(s) 669
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 848
BsaJI CCNNGG 5 cut(s) 180, 990, 991, 1093, 1328
BsaXI ACNNNNNCTCC 2 cut(s) 710, 740
Bsc4I CCNNNNNNNGG 5 cut(s) 589, 628, 817, 1083, 1490
Bse1I ACTGG 6 cut(s) 328, 576, 811, 1315, 1464, 1533
Bse8I GATNNNNATC 1 cut(s) 848
BseBI CCWGG 2 cut(s) 165, 992
BseDI CCNNGG 5 cut(s) 180, 990, 991, 1093, 1328
BseGI GGATG 2 cut(s) 129, 1217
BseJI GATNNNNATC 1 cut(s) 848
BseLI CCNNNNNNNGG 5 cut(s) 589, 628, 817, 1083, 1490
BseMII CTCAG 4 cut(s) 30, 47, 293, 1143
BseNI ACTGG 6 cut(s) 328, 576, 811, 1315, 1464, 1533
BseXI GCAGC 6 cut(s) 774, 796, 1039, 1096, 1372, 1514
BseYI CCCAGC 2 cut(s) 514, 1483
BsgI GTGCAG 1 cut(s) 360
BshFI GGCC 1 cut(s) 1092
BshNI GGYRCC 1 cut(s) 1332
BsiHKAI GWGCWC 2 cut(s) 1024, 1229
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 588, 1518
BslI CCNNNNNNNGG 5 cut(s) 589, 628, 817, 1083, 1490
BsmAI GTCTC 3 cut(s) 622, 1274, 1519
BsmFI GGGAC 2 cut(s) 588, 1518
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 1 cut(s) 1092
Bsp119I TTCGAA 1 cut(s) 669
Bsp1286I GDGCHC 3 cut(s) 1024, 1229, 1324
Bsp143I GATC 5 cut(s) 25, 741, 833, 1148, 1273
Bsp19I CCATGG 2 cut(s) 1093, 1328
BspACI CCGC 7 cut(s) 5, 93, 112, 482, 674, 855, 1292
BspANI GGCC 1 cut(s) 1092
BspCNI CTCAG 4 cut(s) 29, 46, 292, 1144
BspHI TCATGA 1 cut(s) 955
BspLI GGNNCC 5 cut(s) 581, 1275, 1334, 1533, 1534
BspMAI CTGCAG 1 cut(s) 786
BspPI GGATC 3 cut(s) 20, 1268, 1281
BspT104I TTCGAA 1 cut(s) 669
BspT107I GGYRCC 1 cut(s) 1332
BsrI ACTGG 6 cut(s) 328, 576, 811, 1315, 1464, 1533
BssECI CCNNGG 5 cut(s) 180, 990, 991, 1093, 1328
BssMI GATC 5 cut(s) 25, 741, 833, 1148, 1273
BssSI CACGAG 1 cut(s) 98
BssT1I CCWWGG 3 cut(s) 180, 1093, 1328
Bst2BI CACGAG 1 cut(s) 98
Bst2UI CCWGG 2 cut(s) 165, 992
Bst4CI ACNGT 5 cut(s) 240, 864, 913, 1035, 1573
BstBI TTCGAA 1 cut(s) 669
BstC8I GCNNGC 3 cut(s) 496, 1119, 1324
BstDEI CTNAG 7 cut(s) 16, 33, 279, 791, 830, 938, 1152
BstDSI CCRYGG 2 cut(s) 1093, 1328
BstENI CCTNNNNNAGG 1 cut(s) 1081
BstF5I GGATG 2 cut(s) 129, 1217
BstH2I RGCGCY 1 cut(s) 1490
BstHHI GCGC 1 cut(s) 1489
BstKTI GATC 5 cut(s) 28, 744, 836, 1151, 1276
BstMAI GTCTC 3 cut(s) 622, 1274, 1519
BstMBI GATC 5 cut(s) 25, 741, 833, 1148, 1273
BstMWI GCNNNNNNNGC 8 cut(s) 374, 464, 479, 530, 986, 1118, 1197, 1332
BstNI CCWGG 2 cut(s) 165, 992
BstNSI RCATGY 3 cut(s) 1064, 1121, 1462
BstPAI GACNNNNGTC 1 cut(s) 865
BstSCI CCNGG 3 cut(s) 70, 163, 990
BstSFI CTRYAG 4 cut(s) 782, 912, 924, 1569
BstV1I GCAGC 6 cut(s) 774, 796, 1039, 1096, 1372, 1514
BstV2I GAAGAC 2 cut(s) 865, 914
BstX2I RGATCY 1 cut(s) 1273
BstYI RGATCY 1 cut(s) 1273
BsuRI GGCC 1 cut(s) 1092
BtgI CCRYGG 2 cut(s) 1093, 1328
BtgZI GCGATG 1 cut(s) 857
BtsCI GGATG 2 cut(s) 129, 1217
BtsIMutI CAGTG 1 cut(s) 569
Cac8I GCNNGC 3 cut(s) 496, 1119, 1324
CciI TCATGA 1 cut(s) 955
CfoI GCGC 1 cut(s) 1489
Cfr13I GGNCC 4 cut(s) 109, 580, 1090, 1532
CseI GACGC 3 cut(s) 95, 1150, 1206
Csp6I GTAC 1 cut(s) 222
CviQI GTAC 1 cut(s) 222
DdeI CTNAG 7 cut(s) 16, 33, 279, 791, 830, 938, 1152
DpnI GATC 5 cut(s) 27, 743, 835, 1150, 1275
DpnII GATC 5 cut(s) 25, 741, 833, 1148, 1273
EciI GGCGGA 2 cut(s) 20, 82
Ecl136II GAGCTC 1 cut(s) 1227
Eco130I CCWWGG 3 cut(s) 180, 1093, 1328
Eco24I GRGCYC 2 cut(s) 1229, 1324
Eco47I GGWCC 3 cut(s) 109, 580, 1532
Eco47III AGCGCT 1 cut(s) 1488
Eco53kI GAGCTC 1 cut(s) 1227
Eco57I CTGAAG 2 cut(s) 483, 1203
EcoICRI GAGCTC 1 cut(s) 1227
EcoNI CCTNNNNNAGG 1 cut(s) 1081
EcoO109I RGGNCCY 1 cut(s) 1532
EcoRII CCWGG 2 cut(s) 163, 990
EcoT14I CCWWGG 3 cut(s) 180, 1093, 1328
EcoT38I GRGCYC 2 cut(s) 1229, 1324
ErhI CCWWGG 3 cut(s) 180, 1093, 1328
FaqI GGGAC 2 cut(s) 588, 1518
FauI CCCGC 2 cut(s) 475, 1285
FblI GTMKAC 1 cut(s) 21
Fnu4HI GCNGC 6 cut(s) 785, 788, 1053, 1110, 1386, 1503
FokI GGATG 2 cut(s) 116, 1204
FriOI GRGCYC 2 cut(s) 1229, 1324
Fsp4HI GCNGC 6 cut(s) 785, 788, 1053, 1110, 1386, 1503
FspBI CTAG 3 cut(s) 900, 1406, 1594
GlaI GCGC 1 cut(s) 1488
GluI GCNGC 6 cut(s) 785, 788, 1053, 1110, 1386, 1503
GsaI CCCAGC 2 cut(s) 518, 1487
GsuI CTGGAG 1 cut(s) 1158
HaeII RGCGCY 1 cut(s) 1490
HaeIII GGCC 1 cut(s) 1092
HapII CCGG 1 cut(s) 72
HgaI GACGC 3 cut(s) 95, 1150, 1206
HhaI GCGC 1 cut(s) 1489
Hin6I GCGC 1 cut(s) 1487
HinP1I GCGC 1 cut(s) 1487
HincII GTYRAC 2 cut(s) 22, 768
HindII GTYRAC 2 cut(s) 22, 768
HindIII AAGCTT 2 cut(s) 45, 291
HinfI GANTC 6 cut(s) 821, 826, 896, 1282, 1517, 1590
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 1035
Hpy166II GTNNAC 3 cut(s) 22, 768, 1469
Hpy188I TCNGA 9 cut(s) 55, 267, 282, 584, 739, 892, 949, 1153, 1393
Hpy188III TCNNGA 8 cut(s) 41, 107, 347, 956, 983, 1137, 1277, 1556
Hpy8I GTNNAC 3 cut(s) 22, 768, 1469
Hpy99I CGWCG 2 cut(s) 26, 1200
HpyAV CCTTC 3 cut(s) 277, 353, 1010
HpyCH4III ACNGT 5 cut(s) 240, 864, 913, 1035, 1573
HpyCH4IV ACGT 1 cut(s) 308
HpyF10VI GCNNNNNNNGC 8 cut(s) 374, 464, 479, 530, 986, 1118, 1197, 1332
HpyF3I CTNAG 7 cut(s) 16, 33, 279, 791, 830, 938, 1152
HpySE526I ACGT 1 cut(s) 308
HspAI GCGC 1 cut(s) 1487
KflI GGGWCCC 1 cut(s) 1532
Kzo9I GATC 5 cut(s) 25, 741, 833, 1148, 1273
LmnI GCTCC 2 cut(s) 718, 1019
Lsp1109I GCAGC 6 cut(s) 774, 796, 1039, 1096, 1372, 1514
LweI GCATC 7 cut(s) 511, 563, 641, 1168, 1226, 1372, 1561
MaeI CTAG 3 cut(s) 900, 1406, 1594
MaeII ACGT 1 cut(s) 308
MaeIII GTNAC 4 cut(s) 334, 445, 1375, 1576
MalI GATC 5 cut(s) 27, 743, 835, 1150, 1275
MboI GATC 5 cut(s) 25, 741, 833, 1148, 1273
MboII GAAGA 5 cut(s) 20, 42, 870, 919, 1196
MfeI CAATTG 1 cut(s) 1025
MflI RGATCY 1 cut(s) 1273
MhlI GDGCHC 3 cut(s) 1024, 1229, 1324
MluCI AATT 7 cut(s) 274, 548, 585, 877, 1025, 1103, 1426
MlyI GAGTC 2 cut(s) 815, 1276
MmeI TCCRAC 2 cut(s) 53, 1291
MnlI CCTC 5 cut(s) 131, 417, 434, 788, 1346
MroXI GAANNNNTTC 1 cut(s) 608
MseI TTAA 4 cut(s) 320, 681, 707, 1455
MslI CAYNNNNRTG 1 cut(s) 559
MspA1I CMGCKG 4 cut(s) 518, 787, 855, 1052
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 3 cut(s) 72, 165, 992
MunI CAATTG 1 cut(s) 1025
Mva1269I GAATGC 1 cut(s) 327
MvaI CCWGG 2 cut(s) 165, 992
MwoI GCNNNNNNNGC 8 cut(s) 374, 464, 479, 530, 986, 1118, 1197, 1332
NciI CCSGG 1 cut(s) 72
NcoI CCATGG 2 cut(s) 1093, 1328
NdeII GATC 5 cut(s) 25, 741, 833, 1148, 1273
NlaIV GGNNCC 5 cut(s) 581, 1275, 1334, 1533, 1534
NmuCI GTSAC 1 cut(s) 1375
NspI RCATGY 3 cut(s) 1064, 1121, 1462
NspV TTCGAA 1 cut(s) 669
PaeI GCATGC 1 cut(s) 1121
PagI TCATGA 1 cut(s) 955
PasI CCCWGGG 1 cut(s) 991
PciI ACATGT 2 cut(s) 1060, 1458
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 4 cut(s) 826, 896, 1517, 1590
PflFI GACNNNGTC 1 cut(s) 1530
PflMI CCANNNNNTGG 3 cut(s) 628, 817, 1490
PfoI TCCNGGA 1 cut(s) 70
PkrI GCNGC 6 cut(s) 786, 789, 1054, 1111, 1387, 1504
PleI GAGTC 2 cut(s) 815, 1276
PpsI GAGTC 2 cut(s) 815, 1276
PpuMI RGGWCCY 1 cut(s) 1532
PscI ACATGT 2 cut(s) 1060, 1458
PshAI GACNNNNGTC 1 cut(s) 865
PsiI TTATAA 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 1229
Psp5II RGGWCCY 1 cut(s) 1532
Psp6I CCWGG 2 cut(s) 163, 990
PspFI CCCAGC 2 cut(s) 514, 1483
PspGI CCWGG 2 cut(s) 163, 990
PspN4I GGNNCC 5 cut(s) 581, 1275, 1334, 1533, 1534
PspPI GGNCC 4 cut(s) 109, 580, 1090, 1532
PspPPI RGGWCCY 1 cut(s) 1532
PstI CTGCAG 1 cut(s) 786
PsuI RGATCY 1 cut(s) 1273
PsyI GACNNNGTC 1 cut(s) 1530
PvuII CAGCTG 3 cut(s) 518, 787, 1052
RsaI GTAC 1 cut(s) 223
RsaNI GTAC 1 cut(s) 222
RseI CAYNNNNRTG 1 cut(s) 559
SacI GAGCTC 1 cut(s) 1229
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 320, 681, 707, 1455
SatI GCNGC 6 cut(s) 785, 788, 1053, 1110, 1386, 1503
Sau3AI GATC 5 cut(s) 25, 741, 833, 1148, 1273
Sau96I GGNCC 4 cut(s) 109, 580, 1090, 1532
SchI GAGTC 2 cut(s) 815, 1276
ScrFI CCNGG 3 cut(s) 72, 165, 992
SduI GDGCHC 3 cut(s) 1024, 1229, 1324
SfaNI GCATC 7 cut(s) 511, 563, 641, 1168, 1226, 1372, 1561
SfcI CTRYAG 4 cut(s) 782, 912, 924, 1569
SfuI TTCGAA 1 cut(s) 669
SinI GGWCC 3 cut(s) 109, 580, 1532
SmiMI CAYNNNNRTG 1 cut(s) 559
SphI GCATGC 1 cut(s) 1121
Sse9I AATT 7 cut(s) 274, 548, 585, 877, 1025, 1103, 1426
SsiI CCGC 7 cut(s) 5, 93, 112, 482, 674, 855, 1292
SspMI CTAG 3 cut(s) 900, 1406, 1594
SstI GAGCTC 1 cut(s) 1229
StyD4I CCNGG 3 cut(s) 70, 163, 990
StyI CCWWGG 3 cut(s) 180, 1093, 1328
TaaI ACNGT 5 cut(s) 240, 864, 913, 1035, 1573
TaiI ACGT 1 cut(s) 311
TaqI TCGA 9 cut(s) 21, 42, 348, 390, 669, 686, 824, 869, 1147
TasI AATT 7 cut(s) 274, 548, 585, 877, 1025, 1103, 1426
TfiI GAWTC 4 cut(s) 826, 896, 1517, 1590
Tru1I TTAA 4 cut(s) 320, 681, 707, 1455
Tru9I TTAA 4 cut(s) 320, 681, 707, 1455
TscAI CASTG 1 cut(s) 576
TseFI GTSAC 1 cut(s) 1375
TseI GCWGC 6 cut(s) 784, 787, 1052, 1109, 1385, 1502
Tsp45I GTSAC 1 cut(s) 1375
TspDTI ATGAA 8 cut(s) 241, 453, 543, 909, 933, 981, 1224, 1530
TspGWI ACGGA 1 cut(s) 1355
TspRI CASTG 1 cut(s) 576
Tth111I GACNNNGTC 1 cut(s) 1530
Van91I CCANNNNNTGG 3 cut(s) 628, 817, 1490
VpaK11BI GGWCC 3 cut(s) 109, 580, 1532
XagI CCTNNNNNAGG 1 cut(s) 1081
XapI RAATTY 2 cut(s) 274, 585
XceI RCATGY 3 cut(s) 1064, 1121, 1462
XmiI GTMKAC 1 cut(s) 21
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 3 cut(s) 900, 1406, 1594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.