Rh5CG254300

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009552
Physical Location & Seq
Forward (+)
26953937 .. 26961135
7199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG254300.1

Sequence Viewer

Length: 1257 bp
ATGGAGGAAGAATTCTCGGTCGACGACCCAGCTCAGCTTCTCGAAGCTGCTTCAGAGTTCGCAAACTATCCCGGAGTTCAAACCGACGCTTCAGCCAAGGAATTTCTGGACCGCTTCCCTCTCCCTGTCATCCTCAATGCTTTACAGACGAAAGCCGATGTGCCTGGTTTAGAAAATACCTTGGTTGCTGTTTTGGAATGTATTTTCAAAACAAGATATGGTGCTTCTCTCATCCCACATTATATGCCTTTCATACAAGTTGGTCTGACGGCAGATTCTCAGAAAGTCAAATCTCTAGCTTGTAAAACGGTTACTCGCCTTCTCGAAAGTGTGAATGGAGATGCTGTTTCTGCACATCTTATAATTGACAACAATATATATCCTCTATTGCTTGATTGCCTCATCAATGGAAATGAACAAGTTACAACTGTAGCAACTGAAGCAATAAAGAATATAGCTGGTAGTCCAGCAGGCATGGATATTGTTTTCCCAGCTAATACCAATGAAGCTACACATCTTGGAAACTTAGCATCTGAATGCTCACCACTGGGACGGGTTCGAATTTTGGCTTTAATTGTGAAGTTATTCTCTGTCTCCCCCCATGTGGCATCAGTGGTTTCAAAGTCAAATCTACTTAGACTTTTCGAAGCAGAAATTAACTCCAATGATACCCTTGCAACCTTAAATGTTATGGAGCTTTTGTATGAGTTATCAGAGATCAAGCATGGTAGAGTGTTTTTGTCAACAAGCACCCTTCTGCAGCTGCTTAGTTCTATAATAAGCAACCAGTCAATGGACTCAATTCTAAAATCAAGGGCGATGATGATCAGTGGAAGACTGCTGTCGAATGAGAATTACATTTTGTCTGATGAATCCAGTGCGAAGACTGTGGTATTAGCCATAGATAGAATACTTAGTTCATCAGAAACTCATGATACAGATGAATGTGAATCTGCTCTTGAAGCTCTGGGTCAAATAGGGTCATCAATGCAAGGAGCGCAATTGCTACTGTCAAGCTCACCACCAGCTGCAAGACATGTTATCTATTCTTCCTTTGATAGGCAGGGACATGGTAGACAACTGGCTGCCTTGCATGCCCTTGCAAACATCGCTGGAGAAACTCGTTCTGAGAACAGCATAATACTGACTAGTGATGCAGAAGAAAGCCTACGACGCTTGATTTATGAAACCGCATCCAAGAGCTCAAAGTTGACACCATCAGTGAGCAGTTTTTTTTGTCCTCTCTTACTTTTTTAA

Protein Analysis

418

Amino Acids

45.16

Weight (kDa)

4.96

Isoelectric Point (pI)

47.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasom_PSMB PF10508 62 - 340 1.1e-12 Proteasome non-ATPase 26S subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AccB7I CCANNNNNTGG 1 cut(s) 793
AccI GTMKAC 2 cut(s) 21, 1075
AciI CCGC 2 cut(s) 112, 1191
AcsI RAATTY 3 cut(s) 11, 101, 561
AcuI CTGAAG 3 cut(s) 36, 75, 459
AfiI CCNNNNNNNGG 3 cut(s) 604, 793, 1059
AflIII ACRYGT 1 cut(s) 1036
AgsI TTSAA 4 cut(s) 80, 208, 621, 962
AhlI ACTAGT 1 cut(s) 1148
AjnI CCWGG 1 cut(s) 163
Alw21I GWGCWC 1 cut(s) 1205
Alw26I GTCTC 1 cut(s) 598
ApeKI GCWGC 5 cut(s) 47, 760, 763, 1028, 1085
ApoI RAATTY 3 cut(s) 11, 101, 561
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 1 cut(s) 1000
AspS9I GGNCC 1 cut(s) 109
AsuC2I CCSGG 1 cut(s) 72
AsuHPI GGTGA 2 cut(s) 534, 1011
AsuII TTCGAA 2 cut(s) 559, 645
AvaII GGWCC 1 cut(s) 109
BanII GRGCYC 1 cut(s) 1205
BarI GAAGNNNNNNTAC 2 cut(s) 1152, 1184
BbsI GAAGAC 2 cut(s) 841, 890
Bbv12I GWGCWC 1 cut(s) 1205
BbvI GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BccI CCATC 1 cut(s) 1225
BceAI ACGGC 1 cut(s) 285
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 1 cut(s) 72
BcoDI GTCTC 1 cut(s) 598
BcuI ACTAGT 1 cut(s) 1148
BfaI CTAG 2 cut(s) 296, 1149
BfmI CTRYAG 2 cut(s) 429, 758
BisI GCNGC 5 cut(s) 48, 761, 764, 1029, 1086
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 5 cut(s) 49, 762, 765, 1030, 1087
Bme1390I CCNGG 2 cut(s) 72, 165
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 109
BmrFI CCNGG 2 cut(s) 72, 165
BmrI ACTGGG 1 cut(s) 557
BmsI GCATC 5 cut(s) 331, 539, 617, 1144, 1202
BmuI ACTGGG 1 cut(s) 557
BoxI GACNNNNGTC 1 cut(s) 841
BpiI GAAGAC 2 cut(s) 841, 890
BpmI CTGGAG 1 cut(s) 1134
Bpu1102I GCTNAGC 1 cut(s) 33
Bpu14I TTCGAA 2 cut(s) 559, 645
BpuMI CCSGG 1 cut(s) 72
BsaBI GATNNNNATC 1 cut(s) 824
BsaJI CCNNGG 2 cut(s) 96, 180
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 686, 716
Bsc4I CCNNNNNNNGG 3 cut(s) 604, 793, 1059
Bse1I ACTGG 4 cut(s) 552, 787, 876, 1086
Bse8I GATNNNNATC 1 cut(s) 824
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 2 cut(s) 96, 180
BseGI GGATG 3 cut(s) 129, 231, 1193
BseJI GATNNNNATC 1 cut(s) 824
BseLI CCNNNNNNNGG 3 cut(s) 604, 793, 1059
BseMII CTCAG 3 cut(s) 47, 293, 1119
BseNI ACTGG 4 cut(s) 552, 787, 876, 1086
BseXI GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BseYI CCCAGC 2 cut(s) 28, 490
BsgI GTGCAG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 1205
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 564, 1080
BslI CCNNNNNNNGG 3 cut(s) 604, 793, 1059
BsmAI GTCTC 1 cut(s) 598
BsmFI GGGAC 2 cut(s) 564, 1080
BsmI GAATGC 1 cut(s) 542
Bsp119I TTCGAA 2 cut(s) 559, 645
Bsp1286I GDGCHC 1 cut(s) 1205
Bsp143I GATC 2 cut(s) 717, 825
Bsp1720I GCTNAGC 1 cut(s) 33
BspACI CCGC 2 cut(s) 112, 1191
BspCNI CTCAG 3 cut(s) 46, 292, 1120
BspHI TCATGA 1 cut(s) 931
BspMAI CTGCAG 1 cut(s) 762
BspT104I TTCGAA 2 cut(s) 559, 645
BsrI ACTGG 4 cut(s) 552, 787, 876, 1086
BssECI CCNNGG 2 cut(s) 96, 180
BssMI GATC 2 cut(s) 717, 825
BssT1I CCWWGG 2 cut(s) 96, 180
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 4 cut(s) 310, 430, 889, 1011
BstBI TTCGAA 2 cut(s) 559, 645
BstC8I GCNNGC 2 cut(s) 472, 1095
BstDEI CTNAG 7 cut(s) 33, 279, 526, 635, 767, 914, 1128
BstENI CCTNNNNNAGG 1 cut(s) 1057
BstF5I GGATG 3 cut(s) 129, 231, 1193
BstHHI GCGC 1 cut(s) 1000
BstKTI GATC 2 cut(s) 720, 828
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 2 cut(s) 717, 825
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 7 cut(s) 350, 440, 962, 997, 1094, 1109, 1173
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 2 cut(s) 1040, 1097
BstPAI GACNNNNGTC 1 cut(s) 841
BstSCI CCNGG 2 cut(s) 70, 163
BstSFI CTRYAG 2 cut(s) 429, 758
BstV1I GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BstV2I GAAGAC 2 cut(s) 841, 890
BtgZI GCGATG 2 cut(s) 833, 1093
BtsCI GGATG 3 cut(s) 129, 231, 1193
BtsIMutI CAGTG 5 cut(s) 545, 618, 835, 883, 1227
Cac8I GCNNGC 2 cut(s) 472, 1095
CciI TCATGA 1 cut(s) 931
CfoI GCGC 1 cut(s) 1000
Cfr13I GGNCC 1 cut(s) 109
CseI GACGC 2 cut(s) 95, 1182
CviAII CATG 7 cut(s) 475, 602, 725, 932, 1037, 1070, 1094
DdeI CTNAG 7 cut(s) 33, 279, 526, 635, 767, 914, 1128
DpnI GATC 2 cut(s) 719, 827
DpnII GATC 2 cut(s) 717, 825
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 2 cut(s) 96, 180
Eco24I GRGCYC 1 cut(s) 1205
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 3 cut(s) 36, 75, 459
EcoICRI GAGCTC 1 cut(s) 1203
EcoNI CCTNNNNNAGG 1 cut(s) 1057
EcoRI GAATTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 2 cut(s) 96, 180
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 2 cut(s) 96, 180
FaeI CATG 7 cut(s) 478, 605, 728, 935, 1040, 1073, 1097
FaqI GGGAC 2 cut(s) 564, 1080
FatI CATG 7 cut(s) 474, 601, 724, 931, 1036, 1069, 1093
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 2 cut(s) 21, 1075
Fnu4HI GCNGC 5 cut(s) 48, 761, 764, 1029, 1086
FokI GGATG 3 cut(s) 116, 218, 1180
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 5 cut(s) 48, 761, 764, 1029, 1086
FspBI CTAG 2 cut(s) 296, 1149
GlaI GCGC 1 cut(s) 999
GluI GCNGC 5 cut(s) 48, 761, 764, 1029, 1086
GsaI CCCAGC 2 cut(s) 32, 494
GsuI CTGGAG 1 cut(s) 1134
HapII CCGG 1 cut(s) 72
HgaI GACGC 2 cut(s) 95, 1182
HhaI GCGC 1 cut(s) 1000
Hin1II CATG 7 cut(s) 478, 605, 728, 935, 1040, 1073, 1097
Hin6I GCGC 1 cut(s) 998
HinP1I GCGC 1 cut(s) 998
HincII GTYRAC 3 cut(s) 22, 744, 1212
HindII GTYRAC 3 cut(s) 22, 744, 1212
HinfI GANTC 4 cut(s) 275, 797, 872, 950
HpaII CCGG 1 cut(s) 72
HphI GGTGA 2 cut(s) 534, 1011
Hpy166II GTNNAC 4 cut(s) 22, 744, 1076, 1212
Hpy188I TCNGA 8 cut(s) 55, 267, 282, 535, 715, 868, 925, 1129
Hpy188III TCNNGA 5 cut(s) 41, 107, 323, 932, 959
Hpy8I GTNNAC 4 cut(s) 22, 744, 1076, 1212
Hpy99I CGWCG 3 cut(s) 26, 89, 1176
HpyAV CCTTC 2 cut(s) 329, 764
HpyCH4III ACNGT 4 cut(s) 310, 430, 889, 1011
HpyCH4V TGCA 8 cut(s) 353, 677, 760, 991, 1031, 1093, 1103, 1157
HpyF10VI GCNNNNNNNGC 7 cut(s) 350, 440, 962, 997, 1094, 1109, 1173
HpyF3I CTNAG 7 cut(s) 33, 279, 526, 635, 767, 914, 1128
Hsp92II CATG 7 cut(s) 478, 605, 728, 935, 1040, 1073, 1097
HspAI GCGC 1 cut(s) 998
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 2 cut(s) 717, 825
LmnI GCTCC 2 cut(s) 694, 995
Lsp1109I GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
LweI GCATC 5 cut(s) 331, 539, 617, 1144, 1202
MaeI CTAG 2 cut(s) 296, 1149
MaeIII GTNAC 2 cut(s) 310, 421
MalI GATC 2 cut(s) 719, 827
MboI GATC 2 cut(s) 717, 825
MboII GAAGA 5 cut(s) 20, 846, 895, 1041, 1172
MfeI CAATTG 1 cut(s) 1001
MhlI GDGCHC 1 cut(s) 1205
MluCI AATT 9 cut(s) 11, 101, 363, 561, 573, 654, 801, 853, 1001
MlyI GAGTC 1 cut(s) 791
MnlI CCTC 5 cut(s) 129, 143, 393, 410, 1251
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 4 cut(s) 572, 657, 683, 1255
MslI CAYNNNNRTG 1 cut(s) 535
MspA1I CMGCKG 2 cut(s) 763, 1028
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 72, 165
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 7 cut(s) 350, 440, 962, 997, 1094, 1109, 1173
NciI CCSGG 1 cut(s) 72
NdeII GATC 2 cut(s) 717, 825
NlaIII CATG 7 cut(s) 478, 605, 728, 935, 1040, 1073, 1097
NspI RCATGY 2 cut(s) 1040, 1097
NspV TTCGAA 2 cut(s) 559, 645
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 1 cut(s) 931
PciI ACATGT 1 cut(s) 1036
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 3 cut(s) 275, 872, 950
PflMI CCANNNNNTGG 1 cut(s) 793
PfoI TCCNGGA 1 cut(s) 70
PkrI GCNGC 5 cut(s) 49, 762, 765, 1030, 1087
PleI GAGTC 1 cut(s) 791
PpsI GAGTC 1 cut(s) 791
PscI ACATGT 1 cut(s) 1036
PshAI GACNNNNGTC 1 cut(s) 841
PsiI TTATAA 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 1205
Psp6I CCWGG 1 cut(s) 163
PspFI CCCAGC 2 cut(s) 28, 490
PspGI CCWGG 1 cut(s) 163
PspPI GGNCC 1 cut(s) 109
PstI CTGCAG 1 cut(s) 762
PvuII CAGCTG 2 cut(s) 763, 1028
RseI CAYNNNNRTG 1 cut(s) 535
SacI GAGCTC 1 cut(s) 1205
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 572, 657, 683, 1255
SatI GCNGC 5 cut(s) 48, 761, 764, 1029, 1086
Sau3AI GATC 2 cut(s) 717, 825
Sau96I GGNCC 1 cut(s) 109
SchI GAGTC 1 cut(s) 791
ScrFI CCNGG 2 cut(s) 72, 165
SduI GDGCHC 1 cut(s) 1205
SfaNI GCATC 5 cut(s) 331, 539, 617, 1144, 1202
SfcI CTRYAG 2 cut(s) 429, 758
SfuI TTCGAA 2 cut(s) 559, 645
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 1 cut(s) 535
SpeI ACTAGT 1 cut(s) 1148
SphI GCATGC 1 cut(s) 1097
Sse9I AATT 9 cut(s) 11, 101, 363, 561, 573, 654, 801, 853, 1001
SsiI CCGC 2 cut(s) 112, 1191
SspMI CTAG 2 cut(s) 296, 1149
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 2 cut(s) 70, 163
StyI CCWWGG 2 cut(s) 96, 180
TaaI ACNGT 4 cut(s) 310, 430, 889, 1011
TaqI TCGA 6 cut(s) 21, 42, 324, 559, 645, 845
TaqII GACCGA 1 cut(s) 7
TasI AATT 9 cut(s) 11, 101, 363, 561, 573, 654, 801, 853, 1001
TfiI GAWTC 3 cut(s) 275, 872, 950
Tru1I TTAA 4 cut(s) 572, 657, 683, 1255
Tru9I TTAA 4 cut(s) 572, 657, 683, 1255
TscAI CASTG 5 cut(s) 552, 618, 835, 883, 1227
TseI GCWGC 5 cut(s) 47, 760, 763, 1028, 1085
TspDTI ATGAA 7 cut(s) 241, 429, 519, 885, 909, 957, 1200
TspRI CASTG 5 cut(s) 552, 618, 835, 883, 1227
Van91I CCANNNNNTGG 1 cut(s) 793
VpaK11BI GGWCC 1 cut(s) 109
XagI CCTNNNNNAGG 1 cut(s) 1057
XapI RAATTY 3 cut(s) 11, 101, 561
XceI RCATGY 2 cut(s) 1040, 1097
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmiI GTMKAC 2 cut(s) 21, 1075
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 2 cut(s) 296, 1149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.