Rh5BG226800

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009552
Physical Location & Seq
Forward (+)
26605539 .. 26612782
7244 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG226800.1

Sequence Viewer

Length: 1572 bp
ATGGAGGAAGAATTCTCGGTCGACGATCCAGCTCAGCTTCTCGAAGCTGCCTCAGAGTTCGCAAACTATCCCGGAGTTCAAACCGACGCTTCAGCCAAGGAATTTCTGGACCGCTTCCCCCTTCCTGTCATCCTCAGTGCTTTGCAGACGAAAGCCGATGTGCCTGGTTTAGAAAATACCTTGGTTGCTGTTTTGGAATGTATTTTCAAAACAAGATATGGTGCTTCTCTCATCCCACAGTATATGCCTTTCATACAAGTTGGTCTGACGGCAGATTCTCAGAAAGTCAAATCTCTAGCTTGTAAAACGGTTACTCGCCTTCTCGAAAGTGTGAATGGAGATGCTGTTTCTGCACATCTTATAATCGACAACAATATATATCCTCTATTGCTTGATTGCCTCATCAATGGAAATGAACAAGTTACAACTGTAGCAACTGAAGCAATAAAGAATATAGCTGGTAGTCCAGCAGGCATAGATATTGTTTTCCCAGCTAATACCAATGAAGCTACACATCTTGGAAAGTTAGCATCTGAATGCTCACCACTGGGACGGGTTCGAATTTTGGCTTTAATTGTGAAGTTATTCTCTGTCTCCCCCCATGTGGCATCAGTGGTTTCAAAGTCAAATCTACTTAGACTTTTCGAAGCAGAAATTAACTCCAATGATACCCTTGCAACCTTAAATGTTATGGAGCTTTTGTATGAGTTATCAGAGATCAAGCATGGTAGAGTTTTTTTGTCAACAAGCACCCTTCTGCAGCTGCTTAGTTCTATAATAAGCAACCAGTCAATGGACTCAATTCTAACATCAAGGGCGATGATGATCAGTGGAAGACTGCTGTCGAATGAGAATTACATTTTGTCTGATGAATCCAGTGCAAAGACTGTGGTATTAGCCATAGATAGAATACTTAGTTCATCAGAAACTCATGATACAGATGAATGTGAATCTGCTCTTGAAGCTCTGGGTCAAATAGGGTCATCAATGCAAGGAGCGCAATTGCTACTGTCAAGCTCACCGCCAGCTGCAAGACATGTTATCTATTCTTCCTTTGATAGGCAGGGACATGGTAGACAACTGGCTGCCTTGCATGCCCTTGCAAACATCGCTGGAGAAACTCGTTCTGAGAACAGCATAATACTGACTAGTGATGCAGAAGAAAGCCTACGACGCTTGATTTATGAAACCGCATCCAAGAGCTCAAAGTTGACACCATCAGGTATATTCCTCTCAGTTTTCCAACAGGATCCAGAGACTCGTTTGGCGGGTTATAGAGTGCTGACCGGGTTGGCGGCTCGCCCATGGTGCCTCATGGAGATTTGCTCAAAGCAAGAGATAATAAATATAGTGACTGATGCCACTACTGAAACCACAAAAATAGGCATGGAAGCAAGATATAATTGTTGCAAGGCTATCCATACGGCTTTTAACATGTCAAGTAAGCTTTCAAGCGACCCAGCGCTTGGTGGGATTGCGGCAAAGTTGCATGAAGCTGTGAGACTGGGTCCCTATCTTGCTAGAAAGCATCATGAAGCTCAACCTACAGTGGTGACAGCCGACAGATTCTAG

Protein Analysis

523

Amino Acids

56.58

Weight (kDa)

5.34

Isoelectric Point (pI)

43.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasom_PSMB PF10508 62 - 512 3.5e-20 Proteasome non-ATPase 26S subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 362
AccB1I GGYRCC 1 cut(s) 1308
AccB7I CCANNNNNTGG 2 cut(s) 793, 1466
AccI GTMKAC 2 cut(s) 21, 1075
AciI CCGC 6 cut(s) 112, 1022, 1191, 1268, 1295, 1478
AclWI GGATC 3 cut(s) 20, 1244, 1257
AcsI RAATTY 3 cut(s) 11, 101, 561
AcuI CTGAAG 2 cut(s) 75, 459
AfeI AGCGCT 1 cut(s) 1464
AfiI CCNNNNNNNGG 4 cut(s) 604, 793, 1059, 1466
AflIII ACRYGT 2 cut(s) 1036, 1434
AgsI TTSAA 5 cut(s) 80, 208, 621, 962, 1452
AhlI ACTAGT 1 cut(s) 1148
AjnI CCWGG 1 cut(s) 163
Alw21I GWGCWC 1 cut(s) 1205
Alw26I GTCTC 3 cut(s) 598, 1250, 1495
AlwI GGATC 3 cut(s) 20, 1244, 1257
Aor51HI AGCGCT 1 cut(s) 1464
ApeKI GCWGC 5 cut(s) 47, 760, 763, 1028, 1085
ApoI RAATTY 3 cut(s) 11, 101, 561
Asp700I GAANNNNTTC 1 cut(s) 584
AspLEI GCGC 2 cut(s) 1000, 1465
AspS9I GGNCC 2 cut(s) 109, 1508
AsuC2I CCSGG 2 cut(s) 72, 1288
AsuHPI GGTGA 3 cut(s) 534, 1011, 1564
AsuII TTCGAA 2 cut(s) 559, 645
AvaII GGWCC 2 cut(s) 109, 1508
BamHI GGATCC 1 cut(s) 1249
BanI GGYRCC 1 cut(s) 1308
BanII GRGCYC 1 cut(s) 1205
BarI GAAGNNNNNNTAC 2 cut(s) 1152, 1184
BbsI GAAGAC 1 cut(s) 841
Bbv12I GWGCWC 1 cut(s) 1205
BbvI GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BccI CCATC 1 cut(s) 1225
BceAI ACGGC 2 cut(s) 285, 1440
BciT130I CCWGG 1 cut(s) 165
BclI TGATCA 1 cut(s) 825
BcnI CCSGG 2 cut(s) 72, 1288
BcoDI GTCTC 3 cut(s) 598, 1250, 1495
BcuI ACTAGT 1 cut(s) 1148
BfaI CTAG 4 cut(s) 296, 1149, 1521, 1570
BfmI CTRYAG 3 cut(s) 429, 758, 1545
BfoI RGCGCY 1 cut(s) 1466
BisI GCNGC 7 cut(s) 48, 761, 764, 1029, 1086, 1296, 1479
BlpI GCTNAGC 1 cut(s) 33
BlsI GCNGC 7 cut(s) 49, 762, 765, 1030, 1087, 1297, 1480
Bme1390I CCNGG 3 cut(s) 72, 165, 1288
Bme18I GGWCC 2 cut(s) 109, 1508
BmgT120I GGNCC 2 cut(s) 109, 1508
BmiI GGNNCC 4 cut(s) 1251, 1310, 1509, 1510
BmrFI CCNGG 3 cut(s) 72, 165, 1288
BmrI ACTGGG 2 cut(s) 557, 1514
BmsI GCATC 7 cut(s) 331, 539, 617, 1144, 1202, 1348, 1537
BmuI ACTGGG 2 cut(s) 557, 1514
BoxI GACNNNNGTC 1 cut(s) 841
BpiI GAAGAC 1 cut(s) 841
BplI GAGNNNNNCTC 2 cut(s) 1310, 1342
BpmI CTGGAG 1 cut(s) 1134
Bpu1102I GCTNAGC 1 cut(s) 33
Bpu14I TTCGAA 2 cut(s) 559, 645
BpuMI CCSGG 2 cut(s) 72, 1288
BsaBI GATNNNNATC 1 cut(s) 824
BsaJI CCNNGG 3 cut(s) 96, 180, 1304
BsaXI ACNNNNNCTCC 4 cut(s) 330, 360, 686, 716
Bsc4I CCNNNNNNNGG 4 cut(s) 604, 793, 1059, 1466
Bse1I ACTGG 5 cut(s) 552, 787, 876, 1086, 1509
Bse8I GATNNNNATC 1 cut(s) 824
BseBI CCWGG 1 cut(s) 165
BseDI CCNNGG 3 cut(s) 96, 180, 1304
BseGI GGATG 3 cut(s) 129, 231, 1193
BseJI GATNNNNATC 1 cut(s) 824
BseLI CCNNNNNNNGG 4 cut(s) 604, 793, 1059, 1466
BseMII CTCAG 6 cut(s) 47, 66, 148, 293, 1119, 1248
BseNI ACTGG 5 cut(s) 552, 787, 876, 1086, 1509
BseXI GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BseYI CCCAGC 2 cut(s) 490, 1459
BsgI GTGCAG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 21
BshNI GGYRCC 1 cut(s) 1308
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 1205
BsiSI CCGG 2 cut(s) 72, 1287
BslFI GGGAC 3 cut(s) 564, 1080, 1494
BslI CCNNNNNNNGG 4 cut(s) 604, 793, 1059, 1466
BsmAI GTCTC 3 cut(s) 598, 1250, 1495
BsmFI GGGAC 3 cut(s) 564, 1080, 1494
BsmI GAATGC 1 cut(s) 542
Bsp119I TTCGAA 2 cut(s) 559, 645
Bsp1286I GDGCHC 1 cut(s) 1205
Bsp143I GATC 4 cut(s) 25, 717, 825, 1249
Bsp1720I GCTNAGC 1 cut(s) 33
Bsp19I CCATGG 1 cut(s) 1304
BspACI CCGC 6 cut(s) 112, 1022, 1191, 1268, 1295, 1478
BspCNI CTCAG 6 cut(s) 46, 65, 147, 292, 1120, 1247
BspHI TCATGA 2 cut(s) 931, 1531
BspLI GGNNCC 4 cut(s) 1251, 1310, 1509, 1510
BspMAI CTGCAG 1 cut(s) 762
BspPI GGATC 3 cut(s) 20, 1244, 1257
BspT104I TTCGAA 2 cut(s) 559, 645
BspT107I GGYRCC 1 cut(s) 1308
BsrI ACTGG 5 cut(s) 552, 787, 876, 1086, 1509
BssECI CCNNGG 3 cut(s) 96, 180, 1304
BssMI GATC 4 cut(s) 25, 717, 825, 1249
BssT1I CCWWGG 3 cut(s) 96, 180, 1304
Bst2UI CCWGG 1 cut(s) 165
Bst4CI ACNGT 6 cut(s) 240, 310, 430, 889, 1011, 1549
BstBI TTCGAA 2 cut(s) 559, 645
BstC8I GCNNGC 4 cut(s) 472, 1026, 1095, 1300
BstDEI CTNAG 9 cut(s) 33, 52, 134, 279, 635, 767, 914, 1128, 1234
BstDSI CCRYGG 1 cut(s) 1304
BstENI CCTNNNNNAGG 1 cut(s) 1057
BstF5I GGATG 3 cut(s) 129, 231, 1193
BstH2I RGCGCY 1 cut(s) 1466
BstHHI GCGC 2 cut(s) 1000, 1465
BstKTI GATC 4 cut(s) 28, 720, 828, 1252
BstMAI GTCTC 3 cut(s) 598, 1250, 1495
BstMBI GATC 4 cut(s) 25, 717, 825, 1249
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 8 cut(s) 350, 440, 962, 997, 1094, 1109, 1173, 1308
BstNI CCWGG 1 cut(s) 165
BstNSI RCATGY 3 cut(s) 1040, 1097, 1438
BstPAI GACNNNNGTC 1 cut(s) 841
BstSCI CCNGG 3 cut(s) 70, 163, 1286
BstSFI CTRYAG 3 cut(s) 429, 758, 1545
BstV1I GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
BstV2I GAAGAC 1 cut(s) 841
BstX2I RGATCY 1 cut(s) 1249
BstYI RGATCY 1 cut(s) 1249
BtgI CCRYGG 1 cut(s) 1304
BtgZI GCGATG 2 cut(s) 833, 1093
BtsCI GGATG 3 cut(s) 129, 231, 1193
BtsIMutI CAGTG 6 cut(s) 142, 545, 618, 835, 883, 1554
Cac8I GCNNGC 4 cut(s) 472, 1026, 1095, 1300
CciI TCATGA 2 cut(s) 931, 1531
CfoI GCGC 2 cut(s) 1000, 1465
Cfr13I GGNCC 2 cut(s) 109, 1508
CseI GACGC 2 cut(s) 95, 1182
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
DdeI CTNAG 9 cut(s) 33, 52, 134, 279, 635, 767, 914, 1128, 1234
DpnI GATC 4 cut(s) 27, 719, 827, 1251
DpnII GATC 4 cut(s) 25, 717, 825, 1249
Ecl136II GAGCTC 1 cut(s) 1203
Eco130I CCWWGG 3 cut(s) 96, 180, 1304
Eco24I GRGCYC 1 cut(s) 1205
Eco47I GGWCC 2 cut(s) 109, 1508
Eco47III AGCGCT 1 cut(s) 1464
Eco53kI GAGCTC 1 cut(s) 1203
Eco57I CTGAAG 2 cut(s) 75, 459
EcoICRI GAGCTC 1 cut(s) 1203
EcoNI CCTNNNNNAGG 1 cut(s) 1057
EcoO109I RGGNCCY 1 cut(s) 1508
EcoRI GAATTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 163
EcoT14I CCWWGG 3 cut(s) 96, 180, 1304
EcoT38I GRGCYC 1 cut(s) 1205
ErhI CCWWGG 3 cut(s) 96, 180, 1304
FaqI GGGAC 3 cut(s) 564, 1080, 1494
FauI CCCGC 1 cut(s) 1261
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 2 cut(s) 21, 1075
Fnu4HI GCNGC 7 cut(s) 48, 761, 764, 1029, 1086, 1296, 1479
FokI GGATG 3 cut(s) 116, 218, 1180
FriOI GRGCYC 1 cut(s) 1205
Fsp4HI GCNGC 7 cut(s) 48, 761, 764, 1029, 1086, 1296, 1479
FspBI CTAG 4 cut(s) 296, 1149, 1521, 1570
GlaI GCGC 2 cut(s) 999, 1464
GluI GCNGC 7 cut(s) 48, 761, 764, 1029, 1086, 1296, 1479
GsaI CCCAGC 2 cut(s) 494, 1463
GsuI CTGGAG 1 cut(s) 1134
HaeII RGCGCY 1 cut(s) 1466
HapII CCGG 2 cut(s) 72, 1287
HgaI GACGC 2 cut(s) 95, 1182
HhaI GCGC 2 cut(s) 1000, 1465
Hin6I GCGC 2 cut(s) 998, 1463
HinP1I GCGC 2 cut(s) 998, 1463
HincII GTYRAC 3 cut(s) 22, 744, 1212
HindII GTYRAC 3 cut(s) 22, 744, 1212
HindIII AAGCTT 1 cut(s) 1445
HinfI GANTC 6 cut(s) 275, 797, 872, 950, 1258, 1566
HpaII CCGG 2 cut(s) 72, 1287
HphI GGTGA 3 cut(s) 534, 1011, 1564
Hpy166II GTNNAC 4 cut(s) 22, 744, 1076, 1212
Hpy188I TCNGA 8 cut(s) 55, 267, 282, 535, 715, 868, 925, 1129
Hpy188III TCNNGA 7 cut(s) 41, 107, 323, 932, 959, 1253, 1532
Hpy8I GTNNAC 4 cut(s) 22, 744, 1076, 1212
Hpy99I CGWCG 3 cut(s) 26, 89, 1176
HpyAV CCTTC 3 cut(s) 131, 329, 764
HpyCH4III ACNGT 6 cut(s) 240, 310, 430, 889, 1011, 1549
HpyF10VI GCNNNNNNNGC 8 cut(s) 350, 440, 962, 997, 1094, 1109, 1173, 1308
HpyF3I CTNAG 9 cut(s) 33, 52, 134, 279, 635, 767, 914, 1128, 1234
HspAI GCGC 2 cut(s) 998, 1463
KflI GGGWCCC 1 cut(s) 1508
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 4 cut(s) 25, 717, 825, 1249
LmnI GCTCC 2 cut(s) 694, 995
Lsp1109I GCAGC 5 cut(s) 34, 750, 772, 1015, 1072
LweI GCATC 7 cut(s) 331, 539, 617, 1144, 1202, 1348, 1537
MaeI CTAG 4 cut(s) 296, 1149, 1521, 1570
MaeIII GTNAC 4 cut(s) 310, 421, 1351, 1552
MalI GATC 4 cut(s) 27, 719, 827, 1251
MboI GATC 4 cut(s) 25, 717, 825, 1249
MboII GAAGA 4 cut(s) 20, 846, 1041, 1172
MfeI CAATTG 1 cut(s) 1001
MflI RGATCY 1 cut(s) 1249
MhlI GDGCHC 1 cut(s) 1205
MluCI AATT 9 cut(s) 11, 101, 561, 573, 654, 801, 853, 1001, 1402
MlyI GAGTC 2 cut(s) 791, 1252
MmeI TCCRAC 1 cut(s) 1267
MnlI CCTC 6 cut(s) 61, 143, 393, 410, 1241, 1322
MroXI GAANNNNTTC 1 cut(s) 584
MseI TTAA 4 cut(s) 572, 657, 683, 1431
MslI CAYNNNNRTG 1 cut(s) 535
MspA1I CMGCKG 2 cut(s) 763, 1028
MspI CCGG 2 cut(s) 72, 1287
MspR9I CCNGG 3 cut(s) 72, 165, 1288
MunI CAATTG 1 cut(s) 1001
Mva1269I GAATGC 1 cut(s) 542
MvaI CCWGG 1 cut(s) 165
MwoI GCNNNNNNNGC 8 cut(s) 350, 440, 962, 997, 1094, 1109, 1173, 1308
NciI CCSGG 2 cut(s) 72, 1288
NcoI CCATGG 1 cut(s) 1304
NdeII GATC 4 cut(s) 25, 717, 825, 1249
NlaIV GGNNCC 4 cut(s) 1251, 1310, 1509, 1510
NmuCI GTSAC 2 cut(s) 1351, 1552
NspI RCATGY 3 cut(s) 1040, 1097, 1438
NspV TTCGAA 2 cut(s) 559, 645
PaeI GCATGC 1 cut(s) 1097
PagI TCATGA 2 cut(s) 931, 1531
PciI ACATGT 2 cut(s) 1036, 1434
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 584
PfeI GAWTC 4 cut(s) 275, 872, 950, 1566
PflFI GACNNNGTC 1 cut(s) 1506
PflMI CCANNNNNTGG 2 cut(s) 793, 1466
PfoI TCCNGGA 1 cut(s) 70
PkrI GCNGC 7 cut(s) 49, 762, 765, 1030, 1087, 1297, 1480
PleI GAGTC 2 cut(s) 791, 1252
PpsI GAGTC 2 cut(s) 791, 1252
PpuMI RGGWCCY 1 cut(s) 1508
PscI ACATGT 2 cut(s) 1036, 1434
PshAI GACNNNNGTC 1 cut(s) 841
PsiI TTATAA 1 cut(s) 362
Psp124BI GAGCTC 1 cut(s) 1205
Psp5II RGGWCCY 1 cut(s) 1508
Psp6I CCWGG 1 cut(s) 163
PspFI CCCAGC 2 cut(s) 490, 1459
PspGI CCWGG 1 cut(s) 163
PspN4I GGNNCC 4 cut(s) 1251, 1310, 1509, 1510
PspPI GGNCC 2 cut(s) 109, 1508
PspPPI RGGWCCY 1 cut(s) 1508
PstI CTGCAG 1 cut(s) 762
PsuI RGATCY 1 cut(s) 1249
PsyI GACNNNGTC 1 cut(s) 1506
PvuII CAGCTG 2 cut(s) 763, 1028
RseI CAYNNNNRTG 1 cut(s) 535
SacI GAGCTC 1 cut(s) 1205
SalI GTCGAC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 572, 657, 683, 1431
SatI GCNGC 7 cut(s) 48, 761, 764, 1029, 1086, 1296, 1479
Sau3AI GATC 4 cut(s) 25, 717, 825, 1249
Sau96I GGNCC 2 cut(s) 109, 1508
SchI GAGTC 2 cut(s) 791, 1252
ScrFI CCNGG 3 cut(s) 72, 165, 1288
SduI GDGCHC 1 cut(s) 1205
SfaNI GCATC 7 cut(s) 331, 539, 617, 1144, 1202, 1348, 1537
SfcI CTRYAG 3 cut(s) 429, 758, 1545
SfuI TTCGAA 2 cut(s) 559, 645
SinI GGWCC 2 cut(s) 109, 1508
SmiMI CAYNNNNRTG 1 cut(s) 535
SpeI ACTAGT 1 cut(s) 1148
SphI GCATGC 1 cut(s) 1097
Sse9I AATT 9 cut(s) 11, 101, 561, 573, 654, 801, 853, 1001, 1402
SsiI CCGC 6 cut(s) 112, 1022, 1191, 1268, 1295, 1478
SspMI CTAG 4 cut(s) 296, 1149, 1521, 1570
SstI GAGCTC 1 cut(s) 1205
StyD4I CCNGG 3 cut(s) 70, 163, 1286
StyI CCWWGG 3 cut(s) 96, 180, 1304
TaaI ACNGT 6 cut(s) 240, 310, 430, 889, 1011, 1549
TaqI TCGA 7 cut(s) 21, 42, 324, 366, 559, 645, 845
TaqII GACCGA 1 cut(s) 7
TasI AATT 9 cut(s) 11, 101, 561, 573, 654, 801, 853, 1001, 1402
TauI GCSGC 2 cut(s) 1298, 1481
TfiI GAWTC 4 cut(s) 275, 872, 950, 1566
Tru1I TTAA 4 cut(s) 572, 657, 683, 1431
Tru9I TTAA 4 cut(s) 572, 657, 683, 1431
TscAI CASTG 6 cut(s) 142, 552, 618, 835, 883, 1554
TseFI GTSAC 2 cut(s) 1351, 1552
TseI GCWGC 5 cut(s) 47, 760, 763, 1028, 1085
Tsp45I GTSAC 2 cut(s) 1351, 1552
TspDTI ATGAA 9 cut(s) 241, 429, 519, 885, 909, 957, 1200, 1506, 1548
TspRI CASTG 6 cut(s) 142, 552, 618, 835, 883, 1554
Tth111I GACNNNGTC 1 cut(s) 1506
Van91I CCANNNNNTGG 2 cut(s) 793, 1466
VpaK11BI GGWCC 2 cut(s) 109, 1508
XagI CCTNNNNNAGG 1 cut(s) 1057
XapI RAATTY 3 cut(s) 11, 101, 561
XceI RCATGY 3 cut(s) 1040, 1097, 1438
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmiI GTMKAC 2 cut(s) 21, 1075
XmnI GAANNNNTTC 1 cut(s) 584
XspI CTAG 4 cut(s) 296, 1149, 1521, 1570
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.