MD11G1253400.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009552
Physical Location & Seq
Reverse (-)
36548461 .. 36555833
7373 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1253400.v1.1.491

Sequence Viewer

Length: 1515 bp
ATGGAGGAGTACTCGGTGGACGACCCAACTCAGCTACTCGAAGCTGCTTCCGATTTCGCAAGCTACCCCGGTTTTCAAAACGACGCATCAGCTAAGGAATTTCTCGATCGCTTCCCTCTTCCCGTCATTCTCAATGCTTTGCAAACCAAAGCCGATGTGCCCCGTCTGGAAAGCACGTTGGTTGCTGTCTTGGAAAGGGTGTTCAAGACGAAATATGGGGCTTCTCTCATCCCCCAGTTCATGCCATTTGTACAAGTTGGTCTGACGGCAGAGTCTCAAGAAGTCAAATCTTTAGCTTGTAAAACGGTTTCTTGCCTTCTCCAGAATTTGAATGAAGGTTCTGTTTCTGCAACACGCCTTATACTAGACCACAATATATATCCTCTTTTGCTTGATTGCCTCATTCATGGCAATGAAACAGTTGCAACTCTAGCAATGGATGCAATAGCGAAGATAGCTGGTTCTCCTGAAGGCATAGATATTGTTTTCCCAGCTAATAATAAAGAGGCTACACATCTTGGAGCTCTAGCAGACCAATGCTCTTCAGTGGGACGGGTCCGAGTTCTGGCTTTGATAGTGAAGCTATTCTCTATCTCCACCAATGTGGCATCAGTAGTTCAAAAGTCAAATCTACTTGGACTTTTTGAGGCAGAAATCAACAACCCAAATGATACCCTTTCAACCTTGAGCATTTTGGAGCTTTTGTATGAGTTGACAGAGATCGAGCATGGTAGAGAGTTTTTGTCTGCAAGCACCCTTCTGCAACTGCTTAGTTCTATAATCAGCAACAAATCAATGGAGTCGATTTTAAGAACAAGGGCAATGATGATTAGTGGAAGAATTCTGTCCAAGGAGAATTACATACTTGCAGATGAATTGAACTTAAAAACTGTATTATCAGCCATAGACAGGATACTTAGTTCATCCGAAACTCAAGAAATAGATGAATGTGAATCTGCACTTGAAGCACTCGGTCAAATAGGGTCATCAACTCAAGGAGCTCAGCGTTTATTGTCGAGTTCACCACCAGTTGCAAGACATGTTATCTATGCAGCCTTTGATAGACATGGACGTGGTAAACAACTGGCTGCCTTACATGCCCTTGGAAACATTTCTGGGGAAACTCGATCTGCAAACAGCATGATACTGACTAGTGATGCAGAAGAAAGCCTTCGACGCTTGATTTATGAAACAGCATCTAAGAGTTCAAAGCTGACACCATCTGGTCTTTTCCTATCAGTTCTCAAACAGGATTTGGAAATTCGTTTGGCGGGATATAGAATGCTGTCTGGATTGGTGGCTCGCCCATGGTGCCTGATGGAGATTTGCTCAAAGCAAGAGATAATAAATATAGTGACTGATCCAATTACTGAAACCACTAAACTAGGTCTGTTTCACTTTTTCTCCGTAATCATTCAATTTATTACGTTGCAAGTTTATAAATATACAGAATTAACTACATGGAATTGGTTTATACTTTTCTTACAATTTATAACAGTTATTCTTTTCATGTGA

Protein Analysis

505

Amino Acids

55.51

Weight (kDa)

5.17

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasom_PSMB PF10508 61 - 475 1.1e-11 Proteasome non-ATPase 26S subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1440, 1493
AasI GACNNNNNNGTC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 1311
AciI CCGC 1 cut(s) 1271
AclWI GGATC 1 cut(s) 1355
AcsI RAATTY 4 cut(s) 98, 325, 840, 1260
AcuI CTGAAG 2 cut(s) 489, 528
AfaI GTAC 2 cut(s) 11, 252
AfiI CCNNNNNNNGG 2 cut(s) 565, 909
AflIII ACRYGT 1 cut(s) 1039
AgsI TTSAA 9 cut(s) 77, 205, 331, 620, 681, 880, 965, 1209, 1418
AhlI ACTAGT 1 cut(s) 1151
AjiI CACGTC 1 cut(s) 1073
AleI CACNNNNGTG 1 cut(s) 602
AloI GAACNNNNNNTCC 4 cut(s) 185, 217, 904, 936
Alw21I GWGCWC 2 cut(s) 526, 1003
Alw26I GTCTC 1 cut(s) 279
AlwI GGATC 1 cut(s) 1355
ApeKI GCWGC 3 cut(s) 44, 1052, 1088
ApoI RAATTY 4 cut(s) 98, 325, 840, 1260
Asp700I GAANNNNTTC 3 cut(s) 584, 1111, 1170
AspS9I GGNCC 1 cut(s) 556
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 1 cut(s) 1014
AvaII GGWCC 1 cut(s) 556
BaeGI GKGCMC 1 cut(s) 162
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 2 cut(s) 526, 1003
Bbv12I GWGCWC 2 cut(s) 526, 1003
BbvI GCAGC 3 cut(s) 31, 1064, 1075
BccI CCATC 2 cut(s) 1228, 1312
BceAI ACGGC 1 cut(s) 282
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 279
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 5 cut(s) 365, 431, 527, 1152, 1385
BfuI GTATCC 1 cut(s) 906
BisI GCNGC 3 cut(s) 45, 1053, 1089
BlpI GCTNAGC 1 cut(s) 1002
BlsI GCNGC 3 cut(s) 46, 1054, 1090
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 556
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 1 cut(s) 556
BmiI GGNNCC 2 cut(s) 557, 1313
BmrFI CCNGG 1 cut(s) 69
BmrI ACTGGG 1 cut(s) 229
BmsI GCATC 5 cut(s) 95, 430, 617, 1147, 1205
BmuI ACTGGG 1 cut(s) 229
BplI GAGNNNNNCTC 3 cut(s) 28, 1313, 1345
BpmI CTGGAG 1 cut(s) 305
Bpu10I CCTNAGC 1 cut(s) 93
Bpu1102I GCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 4 cut(s) 261, 706, 918, 978
BpuMI CCSGG 1 cut(s) 69
BsaJI CCNNGG 4 cut(s) 67, 849, 1102, 1307
BsaXI ACNNNNNCTCC 4 cut(s) 689, 719, 1388, 1418
Bsc4I CCNNNNNNNGG 2 cut(s) 565, 909
Bse1I ACTGG 3 cut(s) 235, 1028, 1089
Bse3DI GCAATG 3 cut(s) 418, 441, 828
BseDI CCNNGG 4 cut(s) 67, 849, 1102, 1307
BseGI GGATG 3 cut(s) 228, 445, 923
BseLI CCNNNNNNNGG 2 cut(s) 565, 909
BseMI GCAATG 3 cut(s) 418, 441, 828
BseMII CTCAG 2 cut(s) 44, 1016
BseNI ACTGG 3 cut(s) 235, 1028, 1089
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 3 cut(s) 31, 1064, 1075
BseYI CCCAGC 1 cut(s) 490
BsgI GTGCAG 1 cut(s) 942
Bsh1285I CGRYCG 1 cut(s) 109
BshNI GGYRCC 1 cut(s) 1311
BsiEI CGRYCG 1 cut(s) 109
BsiHKAI GWGCWC 2 cut(s) 526, 1003
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 2 cut(s) 565, 909
BsmAI GTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 1287
Bsp1286I GDGCHC 3 cut(s) 162, 526, 1003
Bsp1407I TGTACA 1 cut(s) 250
Bsp143I GATC 4 cut(s) 106, 720, 1127, 1360
Bsp1720I GCTNAGC 1 cut(s) 1002
Bsp19I CCATGG 1 cut(s) 1307
BspACI CCGC 1 cut(s) 1271
BspCNI CTCAG 2 cut(s) 43, 1015
BspLI GGNNCC 2 cut(s) 557, 1313
BspPI GGATC 1 cut(s) 1355
BspQI GCTCTTC 1 cut(s) 547
BspT107I GGYRCC 1 cut(s) 1311
BsrDI GCAATG 3 cut(s) 418, 441, 828
BsrGI TGTACA 1 cut(s) 250
BsrI ACTGG 3 cut(s) 235, 1028, 1089
BssECI CCNNGG 4 cut(s) 67, 849, 1102, 1307
BssMI GATC 4 cut(s) 106, 720, 1127, 1360
BssT1I CCWWGG 3 cut(s) 849, 1102, 1307
Bst4CI ACNGT 4 cut(s) 307, 421, 892, 1498
Bst6I CTCTTC 2 cut(s) 123, 547
BstAPI GCANNNNNTGC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 250
BstC8I GCNNGC 3 cut(s) 61, 751, 1303
BstDEI CTNAG 6 cut(s) 30, 93, 770, 917, 1002, 1200
BstDSI CCRYGG 1 cut(s) 1307
BstF5I GGATG 3 cut(s) 228, 445, 923
BstKTI GATC 4 cut(s) 109, 723, 1130, 1363
BstMAI GTCTC 1 cut(s) 279
BstMBI GATC 4 cut(s) 106, 720, 1127, 1360
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 7 cut(s) 431, 440, 455, 965, 1097, 1176, 1311
BstNSI RCATGY 2 cut(s) 1043, 1100
BstSCI CCNGG 1 cut(s) 67
BstSLI GKGCMC 1 cut(s) 162
BstV1I GCAGC 3 cut(s) 31, 1064, 1075
BstXI CCANNNNNNTGG 1 cut(s) 604
BsuI GTATCC 1 cut(s) 906
BtgI CCRYGG 1 cut(s) 1307
BtrI CACGTC 1 cut(s) 1073
BtsCI GGATG 3 cut(s) 228, 445, 923
BtsIMutI CAGTG 1 cut(s) 552
Cac8I GCNNGC 3 cut(s) 61, 751, 1303
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 2 cut(s) 92, 1185
Csp6I GTAC 2 cut(s) 10, 251
CviQI GTAC 2 cut(s) 10, 251
DdeI CTNAG 6 cut(s) 30, 93, 770, 917, 1002, 1200
DpnI GATC 4 cut(s) 108, 722, 1129, 1362
DpnII GATC 4 cut(s) 106, 720, 1127, 1360
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
Eam1104I CTCTTC 2 cut(s) 123, 547
EarI CTCTTC 2 cut(s) 123, 547
Ecl136II GAGCTC 2 cut(s) 524, 1001
Eco130I CCWWGG 3 cut(s) 849, 1102, 1307
Eco24I GRGCYC 2 cut(s) 526, 1003
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 2 cut(s) 524, 1001
Eco57I CTGAAG 2 cut(s) 489, 528
EcoICRI GAGCTC 2 cut(s) 524, 1001
EcoRI GAATTC 1 cut(s) 840
EcoT14I CCWWGG 3 cut(s) 849, 1102, 1307
EcoT38I GRGCYC 2 cut(s) 526, 1003
ErhI CCWWGG 3 cut(s) 849, 1102, 1307
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FaqI GGGAC 1 cut(s) 564
FauI CCCGC 1 cut(s) 1264
Fnu4HI GCNGC 3 cut(s) 45, 1053, 1089
FokI GGATG 3 cut(s) 215, 452, 910
FriOI GRGCYC 2 cut(s) 526, 1003
Fsp4HI GCNGC 3 cut(s) 45, 1053, 1089
FspBI CTAG 5 cut(s) 365, 431, 527, 1152, 1385
GluI GCNGC 3 cut(s) 45, 1053, 1089
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 1 cut(s) 305
HapII CCGG 1 cut(s) 69
HgaI GACGC 2 cut(s) 92, 1185
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HinfI GANTC 3 cut(s) 272, 800, 953
HpaII CCGG 1 cut(s) 69
HphI GGTGA 1 cut(s) 1014
Hpy166II GTNNAC 4 cut(s) 19, 714, 1022, 1079
Hpy188I TCNGA 4 cut(s) 52, 264, 560, 928
Hpy188III TCNNGA 8 cut(s) 104, 167, 205, 278, 322, 467, 935, 1290
Hpy8I GTNNAC 4 cut(s) 19, 714, 1022, 1079
Hpy99I CGWCG 2 cut(s) 86, 1179
HpyAV CCTTC 5 cut(s) 326, 329, 464, 767, 1181
HpyCH4III ACNGT 4 cut(s) 307, 421, 892, 1498
HpyCH4IV ACGT 3 cut(s) 176, 1072, 1427
HpyF10VI GCNNNNNNNGC 7 cut(s) 431, 440, 455, 965, 1097, 1176, 1311
HpyF3I CTNAG 6 cut(s) 30, 93, 770, 917, 1002, 1200
HpySE526I ACGT 3 cut(s) 176, 1072, 1427
Kzo9I GATC 4 cut(s) 106, 720, 1127, 1360
LguI GCTCTTC 1 cut(s) 547
LmnI GCTCC 3 cut(s) 521, 697, 998
Lsp1109I GCAGC 3 cut(s) 31, 1064, 1075
LweI GCATC 5 cut(s) 95, 430, 617, 1147, 1205
MaeI CTAG 5 cut(s) 365, 431, 527, 1152, 1385
MaeII ACGT 3 cut(s) 176, 1072, 1427
MaeIII GTNAC 1 cut(s) 1354
MalI GATC 4 cut(s) 108, 722, 1129, 1362
MboI GATC 4 cut(s) 106, 720, 1127, 1360
MboII GAAGA 5 cut(s) 110, 463, 534, 849, 1175
MhlI GDGCHC 3 cut(s) 162, 526, 1003
MlyI GAGTC 2 cut(s) 281, 809
MnlI CCTC 5 cut(s) 126, 393, 410, 499, 640
MroXI GAANNNNTTC 3 cut(s) 584, 1111, 1170
MseI TTAA 3 cut(s) 809, 884, 1454
MslI CAYNNNNRTG 3 cut(s) 411, 602, 1071
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 1287
MwoI GCNNNNNNNGC 7 cut(s) 431, 440, 455, 965, 1097, 1176, 1311
NciI CCSGG 1 cut(s) 69
NcoI CCATGG 1 cut(s) 1307
NdeII GATC 4 cut(s) 106, 720, 1127, 1360
NlaIV GGNNCC 2 cut(s) 557, 1313
NmuCI GTSAC 1 cut(s) 1354
NspI RCATGY 2 cut(s) 1043, 1100
OliI CACNNNNGTG 1 cut(s) 602
PciI ACATGT 1 cut(s) 1039
PciSI GCTCTTC 1 cut(s) 547
PctI GAATGC 1 cut(s) 1287
PdmI GAANNNNTTC 3 cut(s) 584, 1111, 1170
PfeI GAWTC 1 cut(s) 953
PkrI GCNGC 3 cut(s) 46, 1054, 1090
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 2 cut(s) 280, 808
PpsI GAGTC 2 cut(s) 280, 808
PscI ACATGT 1 cut(s) 1039
PsiI TTATAA 2 cut(s) 1440, 1493
Psp124BI GAGCTC 2 cut(s) 526, 1003
PspFI CCCAGC 1 cut(s) 490
PspN4I GGNNCC 2 cut(s) 557, 1313
PspPI GGNCC 1 cut(s) 556
PvuI CGATCG 1 cut(s) 109
RsaI GTAC 2 cut(s) 11, 252
RsaNI GTAC 2 cut(s) 10, 251
RseI CAYNNNNRTG 3 cut(s) 411, 602, 1071
SacI GAGCTC 2 cut(s) 526, 1003
SapI GCTCTTC 1 cut(s) 547
SaqAI TTAA 3 cut(s) 809, 884, 1454
SatI GCNGC 3 cut(s) 45, 1053, 1089
Sau3AI GATC 4 cut(s) 106, 720, 1127, 1360
Sau96I GGNCC 1 cut(s) 556
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 281, 809
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 3 cut(s) 162, 526, 1003
SfaNI GCATC 5 cut(s) 95, 430, 617, 1147, 1205
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 3 cut(s) 411, 602, 1071
SmlI CTYRAG 4 cut(s) 276, 685, 933, 993
SmoI CTYRAG 4 cut(s) 276, 685, 933, 993
SpeI ACTAGT 1 cut(s) 1151
SsiI CCGC 1 cut(s) 1271
SspMI CTAG 5 cut(s) 365, 431, 527, 1152, 1385
SstI GAGCTC 2 cut(s) 526, 1003
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 3 cut(s) 849, 1102, 1307
TaaI ACNGT 4 cut(s) 307, 421, 892, 1498
TaiI ACGT 3 cut(s) 179, 1075, 1430
TaqI TCGA 7 cut(s) 39, 105, 723, 803, 1016, 1126, 1174
TaqII GACCGA 1 cut(s) 962
TatI WGTACW 2 cut(s) 9, 250
TfiI GAWTC 1 cut(s) 953
Tru1I TTAA 3 cut(s) 809, 884, 1454
Tru9I TTAA 3 cut(s) 809, 884, 1454
TscAI CASTG 1 cut(s) 552
TseFI GTSAC 1 cut(s) 1354
TseI GCWGC 3 cut(s) 44, 1052, 1088
Tsp45I GTSAC 1 cut(s) 1354
TspDTI ATGAA 9 cut(s) 229, 348, 395, 429, 888, 912, 960, 1203, 1498
TspGWI ACGGA 1 cut(s) 1396
TspRI CASTG 1 cut(s) 552
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 4 cut(s) 98, 325, 840, 1260
XceI RCATGY 2 cut(s) 1043, 1100
XmnI GAANNNNTTC 3 cut(s) 584, 1111, 1170
XspI CTAG 5 cut(s) 365, 431, 527, 1152, 1385
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.