MD03G1232700.v1.1

26S proteasome non-ATPase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009552
Physical Location & Seq
Reverse (-)
31749332 .. 31757469
8138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1232700.v1.1.491

Sequence Viewer

Length: 1575 bp
ATGGAGGAGTACTCGGTAGACGACCCAACTCAGCTACTCGAAGCTGCTTCCGATTTCGCAAACTACCCCGGTTTTCAAAACGACGATTCAGCTAAGGAATTCCTCGATCGCTTCCCACTTCCTGTCATTCTCAATGCTTTGCAAACGAAAGCCGATGTGCCGGGTCTGGAAAGCACACTGGTTGCTGTTTTGGAAAGGGTGTTCAAGACCAAATATGGCGCTTCTCTCATCCCCCATTTCATGCCATTTGTACAAGTTGGTCTGACGGCAGAGTCTCAGGAAGTCAAATCTTTAGCTTGTACAACGGTTTCTTGTCTTCTCGAGAATTTGAATGAAGGTTCTGTTTCTGCAACACGCCTTATACTTTACCACAATATATATCCTCTTTTGCTGGATTGCCTCATTCATGGCAATGAAAAAGTTGCAACTCTAGCAATGGATGCAATAGAGAAGATAGCTGGTTCTCCCGAGGGCATAGATATTGTCTTCCCGGCTAATACTAACGAAGCTACACATCTTGGGGCTCTATCAGCCCAATGCTCGTCAGTGGGACGGGTCCGGGTTCTGGCTTTGATAGTGAAGCTAGTCTCTGTGTCCGCTGATGTAGCATCAGTAGTTCAAAAGTCAAATCTACTTGGACTTTTTGAGGCAGAAATCAATAACAGAACTGATACCCTTTCAACCTTGAGCATTTTGGAACTTTTGTATGAGTTGTCAGAGATCGAGCATGATAGAGAGTTTTTGTCTGCAAGCACCCTTCTGCAACTACTTAGTTCTATAATCAGCAACAAATCAATGGAGTCGATTTTAAGAACAAGGGCAATGATGATTAGTGGAAGAATTCTGTCCAAGGAGAATTACATACATGCTGATGAATTAAGTGTAAAAACTGTATTATCGGCCATAGACGGGATACTTAGTTCATCCGAAACTCAAGAAATAGATGAATGTGAATCTGCTATTGAAGCACTGGGTCAAATAGGGTCATCAATTCAAGGAGCGCAGTTTCTATTGTCAAGTTCACCACCAGCTGCAAGACATGTTATCTATGCTGCCTTTGATAGGCACGGACATGGTAAACAACTGGCTGCCTTACATGCCCTTGGAAACATCTCTGGAGAAACTCGGCCTGCGAACAGCATGATACTGACTAGTGATGCAGAAGAAAGCCTTCGACGCTTGATTTATGAAACAGCATCTAAGAGTTCAAAGCTGACACCATCTGGCCTTTTCCTATCAGTTCTCAAACAGGATTCAGAAATTCGTTTGGCGGGATATCGAATGCTGTCTGGGTTGGTTGCTCGTCCATGGTGCCTGATGGAGATTTGCTCAAAGCAAGAGATAATAAATATAGTGACTGATCCAATTACTGAAACCACTAAACTAGGTATGGAAGCCAGATATAATTGCTGCAAGGCAATCCACACCGCTTTTAACATGTCAAGTAAACTTGCCAGCGACCAATCTCTTTCTGGGATTGCGGCAAAGTTGCACGAAGCTGTTAAAATGGGTCCGTACCTTGCTAGTAAGCACCTTGAGGCTCAACCCATGGTGGTGACGGAGGACAGATTCTAG

Protein Analysis

525

Amino Acids

56.92

Weight (kDa)

5.14

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proteasom_PSMB PF10508 61 - 513 1.2e-13 Proteasome non-ATPase 26S subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 1311
AccI GTMKAC 1 cut(s) 18
AciI CCGC 4 cut(s) 597, 1271, 1428, 1481
AclWI GGATC 1 cut(s) 1355
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 4 cut(s) 98, 325, 840, 1260
AfaI GTAC 4 cut(s) 11, 252, 301, 1517
AfiI CCNNNNNNNGG 3 cut(s) 565, 909, 1062
AflIII ACRYGT 2 cut(s) 1039, 1437
AgsI TTSAA 8 cut(s) 77, 205, 331, 620, 681, 965, 995, 1209
AhlI ACTAGT 1 cut(s) 1151
AloI GAACNNNNNNTCC 4 cut(s) 185, 217, 904, 936
Alw26I GTCTC 2 cut(s) 279, 592
AlwI GGATC 1 cut(s) 1355
Ama87I CYCGRG 2 cut(s) 320, 467
AoxI GGCC 3 cut(s) 900, 1127, 1225
ApeKI GCWGC 5 cut(s) 44, 1031, 1052, 1088, 1410
ApoI RAATTY 4 cut(s) 98, 325, 840, 1260
Asp700I GAANNNNTTC 1 cut(s) 1170
AspLEI GCGC 2 cut(s) 221, 1003
AspS9I GGNCC 2 cut(s) 556, 1511
AsuC2I CCSGG 4 cut(s) 69, 162, 491, 560
AsuHPI GGTGA 2 cut(s) 1014, 1567
AvaI CYCGRG 2 cut(s) 320, 467
AvaII GGWCC 2 cut(s) 556, 1511
BanI GGYRCC 1 cut(s) 1311
BanII GRGCYC 1 cut(s) 526
BbsI GAAGAC 2 cut(s) 308, 478
BbvI GCAGC 5 cut(s) 31, 1018, 1039, 1075, 1397
BccI CCATC 2 cut(s) 1228, 1312
BceAI ACGGC 1 cut(s) 282
BciVI GTATCC 1 cut(s) 906
BcnI CCSGG 4 cut(s) 69, 162, 491, 560
BcoDI GTCTC 2 cut(s) 279, 592
BcuI ACTAGT 1 cut(s) 1151
BfaI CTAG 6 cut(s) 431, 584, 1152, 1385, 1524, 1573
BfoI RGCGCY 1 cut(s) 222
BfuI GTATCC 1 cut(s) 906
BisI GCNGC 6 cut(s) 45, 1032, 1053, 1089, 1411, 1482
BlsI GCNGC 6 cut(s) 46, 1033, 1054, 1090, 1412, 1483
BmcAI AGTACT 1 cut(s) 11
Bme1390I CCNGG 4 cut(s) 69, 162, 491, 560
Bme18I GGWCC 2 cut(s) 556, 1511
BmeT110I CYCGRG 2 cut(s) 320, 467
BmgT120I GGNCC 2 cut(s) 556, 1511
BmiI GGNNCC 3 cut(s) 557, 1313, 1512
BmrFI CCNGG 4 cut(s) 69, 162, 491, 560
BmrI ACTGGG 1 cut(s) 980
BmsI GCATC 4 cut(s) 430, 617, 1147, 1205
BmuI ACTGGG 1 cut(s) 980
BpiI GAAGAC 2 cut(s) 308, 478
BplI GAGNNNNNCTC 3 cut(s) 28, 1313, 1345
BpmI CTGGAG 1 cut(s) 1137
Bpu10I CCTNAGC 1 cut(s) 93
BpuEI CTTGAG 3 cut(s) 706, 918, 1556
BpuMI CCSGG 4 cut(s) 69, 162, 491, 560
BsaJI CCNNGG 6 cut(s) 67, 468, 849, 1102, 1307, 1548
Bsc4I CCNNNNNNNGG 3 cut(s) 565, 909, 1062
Bse1I ACTGG 3 cut(s) 183, 975, 1089
Bse3DI GCAATG 3 cut(s) 418, 441, 828
BseDI CCNNGG 6 cut(s) 67, 468, 849, 1102, 1307, 1548
BseGI GGATG 3 cut(s) 228, 445, 923
BseLI CCNNNNNNNGG 3 cut(s) 565, 909, 1062
BseMI GCAATG 3 cut(s) 418, 441, 828
BseMII CTCAG 2 cut(s) 44, 290
BseNI ACTGG 3 cut(s) 183, 975, 1089
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 5 cut(s) 31, 1018, 1039, 1075, 1397
Bsh1285I CGRYCG 1 cut(s) 109
BshFI GGCC 3 cut(s) 902, 1129, 1227
BshNI GGYRCC 1 cut(s) 1311
BsiEI CGRYCG 1 cut(s) 109
BsiHKCI CYCGRG 2 cut(s) 320, 467
BsiSI CCGG 4 cut(s) 69, 161, 491, 559
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 3 cut(s) 565, 909, 1062
BsmAI GTCTC 2 cut(s) 279, 592
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 1287
BsnI GGCC 3 cut(s) 902, 1129, 1227
BsoBI CYCGRG 2 cut(s) 320, 467
Bsp1286I GDGCHC 1 cut(s) 526
Bsp1407I TGTACA 2 cut(s) 250, 299
Bsp143I GATC 3 cut(s) 106, 720, 1360
Bsp19I CCATGG 2 cut(s) 1307, 1548
BspACI CCGC 4 cut(s) 597, 1271, 1428, 1481
BspANI GGCC 3 cut(s) 902, 1129, 1227
BspCNI CTCAG 2 cut(s) 43, 289
BspLI GGNNCC 3 cut(s) 557, 1313, 1512
BspPI GGATC 1 cut(s) 1355
BspT107I GGYRCC 1 cut(s) 1311
BsrDI GCAATG 3 cut(s) 418, 441, 828
BsrGI TGTACA 2 cut(s) 250, 299
BsrI ACTGG 3 cut(s) 183, 975, 1089
BssECI CCNNGG 6 cut(s) 67, 468, 849, 1102, 1307, 1548
BssMI GATC 3 cut(s) 106, 720, 1360
BssT1I CCWWGG 4 cut(s) 849, 1102, 1307, 1548
Bst4CI ACNGT 2 cut(s) 307, 892
BstAPI GCANNNNNTGC 1 cut(s) 440
BstAUI TGTACA 2 cut(s) 250, 299
BstC8I GCNNGC 3 cut(s) 751, 1131, 1456
BstDEI CTNAG 6 cut(s) 30, 93, 276, 770, 917, 1200
BstDSI CCRYGG 2 cut(s) 1307, 1548
BstENI CCTNNNNNAGG 1 cut(s) 1060
BstF5I GGATG 3 cut(s) 228, 445, 923
BstH2I RGCGCY 1 cut(s) 222
BstHHI GCGC 2 cut(s) 221, 1003
BstKTI GATC 3 cut(s) 109, 723, 1363
BstMAI GTCTC 2 cut(s) 279, 592
BstMBI GATC 3 cut(s) 106, 720, 1360
BstMCI CGRYCG 1 cut(s) 109
BstMWI GCNNNNNNNGC 7 cut(s) 431, 440, 530, 605, 965, 1097, 1176
BstNSI RCATGY 4 cut(s) 869, 1043, 1100, 1441
BstSCI CCNGG 4 cut(s) 67, 160, 489, 558
BstV1I GCAGC 5 cut(s) 31, 1018, 1039, 1075, 1397
BstV2I GAAGAC 2 cut(s) 308, 478
BsuI GTATCC 1 cut(s) 906
BsuRI GGCC 3 cut(s) 902, 1129, 1227
BtgI CCRYGG 2 cut(s) 1307, 1548
BtsCI GGATG 3 cut(s) 228, 445, 923
BtsIMutI CAGTG 3 cut(s) 176, 552, 968
Cac8I GCNNGC 3 cut(s) 751, 1131, 1456
CfoI GCGC 2 cut(s) 221, 1003
Cfr13I GGNCC 2 cut(s) 556, 1511
CseI GACGC 1 cut(s) 1185
Csp6I GTAC 4 cut(s) 10, 251, 300, 1516
CviQI GTAC 4 cut(s) 10, 251, 300, 1516
DdeI CTNAG 6 cut(s) 30, 93, 276, 770, 917, 1200
DpnI GATC 3 cut(s) 108, 722, 1362
DpnII GATC 3 cut(s) 106, 720, 1360
DrdI GACNNNNNNGTC 1 cut(s) 271
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 900
Eco130I CCWWGG 4 cut(s) 849, 1102, 1307, 1548
Eco24I GRGCYC 1 cut(s) 526
Eco32I GATATC 1 cut(s) 1277
Eco47I GGWCC 2 cut(s) 556, 1511
Eco88I CYCGRG 2 cut(s) 320, 467
EcoNI CCTNNNNNAGG 1 cut(s) 1060
EcoRI GAATTC 2 cut(s) 98, 840
EcoRV GATATC 1 cut(s) 1277
EcoT14I CCWWGG 4 cut(s) 849, 1102, 1307, 1548
EcoT38I GRGCYC 1 cut(s) 526
ErhI CCWWGG 4 cut(s) 849, 1102, 1307, 1548
FalI AAGNNNNNCTT 2 cut(s) 1155, 1187
FaqI GGGAC 1 cut(s) 564
FauI CCCGC 1 cut(s) 1264
FblI GTMKAC 1 cut(s) 18
Fnu4HI GCNGC 6 cut(s) 45, 1032, 1053, 1089, 1411, 1482
FokI GGATG 3 cut(s) 215, 452, 910
FriOI GRGCYC 1 cut(s) 526
Fsp4HI GCNGC 6 cut(s) 45, 1032, 1053, 1089, 1411, 1482
FspBI CTAG 6 cut(s) 431, 584, 1152, 1385, 1524, 1573
GlaI GCGC 2 cut(s) 220, 1002
GluI GCNGC 6 cut(s) 45, 1032, 1053, 1089, 1411, 1482
GsuI CTGGAG 1 cut(s) 1137
HaeII RGCGCY 1 cut(s) 222
HaeIII GGCC 3 cut(s) 902, 1129, 1227
HapII CCGG 4 cut(s) 69, 161, 491, 559
HgaI GACGC 1 cut(s) 1185
HhaI GCGC 2 cut(s) 221, 1003
Hin6I GCGC 2 cut(s) 219, 1001
HinP1I GCGC 2 cut(s) 219, 1001
HinfI GANTC 6 cut(s) 86, 272, 800, 953, 1253, 1569
HpaII CCGG 4 cut(s) 69, 161, 491, 559
HphI GGTGA 2 cut(s) 1014, 1567
Hpy166II GTNNAC 4 cut(s) 19, 1022, 1079, 1448
Hpy188I TCNGA 5 cut(s) 52, 264, 718, 928, 1258
Hpy188III TCNNGA 8 cut(s) 167, 205, 278, 320, 322, 467, 935, 1116
Hpy8I GTNNAC 4 cut(s) 19, 1022, 1079, 1448
Hpy99I CGWCG 2 cut(s) 86, 1179
HpyAV CCTTC 3 cut(s) 329, 767, 1181
HpyCH4III ACNGT 2 cut(s) 307, 892
HpyF10VI GCNNNNNNNGC 7 cut(s) 431, 440, 530, 605, 965, 1097, 1176
HpyF3I CTNAG 6 cut(s) 30, 93, 276, 770, 917, 1200
HspAI GCGC 2 cut(s) 219, 1001
Kzo9I GATC 3 cut(s) 106, 720, 1360
LmnI GCTCC 1 cut(s) 998
Lsp1109I GCAGC 5 cut(s) 31, 1018, 1039, 1075, 1397
LweI GCATC 4 cut(s) 430, 617, 1147, 1205
MaeI CTAG 6 cut(s) 431, 584, 1152, 1385, 1524, 1573
MaeIII GTNAC 2 cut(s) 1354, 1555
MalI GATC 3 cut(s) 108, 722, 1362
MboI GATC 3 cut(s) 106, 720, 1360
MboII GAAGA 5 cut(s) 308, 463, 478, 849, 1175
MhlI GDGCHC 1 cut(s) 526
MluCI AATT 9 cut(s) 98, 325, 840, 856, 875, 990, 1260, 1365, 1405
MlyI GAGTC 2 cut(s) 281, 809
MnlI CCTC 7 cut(s) 113, 393, 410, 463, 640, 1531, 1555
MroXI GAANNNNTTC 1 cut(s) 1170
MseI TTAA 4 cut(s) 809, 878, 1434, 1503
MslI CAYNNNNRTG 4 cut(s) 411, 870, 1071, 1553
MspA1I CMGCKG 2 cut(s) 599, 1031
MspI CCGG 4 cut(s) 69, 161, 491, 559
MspR9I CCNGG 4 cut(s) 69, 162, 491, 560
Mva1269I GAATGC 1 cut(s) 1287
MwoI GCNNNNNNNGC 7 cut(s) 431, 440, 530, 605, 965, 1097, 1176
NciI CCSGG 4 cut(s) 69, 162, 491, 560
NcoI CCATGG 2 cut(s) 1307, 1548
NdeII GATC 3 cut(s) 106, 720, 1360
NlaIV GGNNCC 3 cut(s) 557, 1313, 1512
NmeAIII GCCGAG 1 cut(s) 1105
NmuCI GTSAC 2 cut(s) 1354, 1555
NspI RCATGY 4 cut(s) 869, 1043, 1100, 1441
PaeR7I CTCGAG 1 cut(s) 320
PciI ACATGT 2 cut(s) 1039, 1437
PctI GAATGC 1 cut(s) 1287
PdmI GAANNNNTTC 1 cut(s) 1170
PfeI GAWTC 4 cut(s) 86, 953, 1253, 1569
PkrI GCNGC 6 cut(s) 46, 1033, 1054, 1090, 1412, 1483
Ple19I CGATCG 1 cut(s) 109
PleI GAGTC 2 cut(s) 280, 808
PpsI GAGTC 2 cut(s) 280, 808
PscI ACATGT 2 cut(s) 1039, 1437
PspN4I GGNNCC 3 cut(s) 557, 1313, 1512
PspPI GGNCC 2 cut(s) 556, 1511
PvuI CGATCG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 11, 252, 301, 1517
RsaNI GTAC 4 cut(s) 10, 251, 300, 1516
RseI CAYNNNNRTG 4 cut(s) 411, 870, 1071, 1553
SaqAI TTAA 4 cut(s) 809, 878, 1434, 1503
SatI GCNGC 6 cut(s) 45, 1032, 1053, 1089, 1411, 1482
Sau3AI GATC 3 cut(s) 106, 720, 1360
Sau96I GGNCC 2 cut(s) 556, 1511
ScaI AGTACT 1 cut(s) 11
SchI GAGTC 2 cut(s) 281, 809
ScrFI CCNGG 4 cut(s) 69, 162, 491, 560
SduI GDGCHC 1 cut(s) 526
SfaNI GCATC 4 cut(s) 430, 617, 1147, 1205
Sfr274I CTCGAG 1 cut(s) 320
SinI GGWCC 2 cut(s) 556, 1511
SlaI CTCGAG 1 cut(s) 320
SmiMI CAYNNNNRTG 4 cut(s) 411, 870, 1071, 1553
SmlI CTYRAG 4 cut(s) 320, 685, 933, 1535
SmoI CTYRAG 4 cut(s) 320, 685, 933, 1535
SpeI ACTAGT 1 cut(s) 1151
Sse9I AATT 9 cut(s) 98, 325, 840, 856, 875, 990, 1260, 1365, 1405
SsiI CCGC 4 cut(s) 597, 1271, 1428, 1481
SspMI CTAG 6 cut(s) 431, 584, 1152, 1385, 1524, 1573
StyD4I CCNGG 4 cut(s) 67, 160, 489, 558
StyI CCWWGG 4 cut(s) 849, 1102, 1307, 1548
TaaI ACNGT 2 cut(s) 307, 892
TaqI TCGA 7 cut(s) 39, 105, 321, 723, 803, 1174, 1279
TasI AATT 9 cut(s) 98, 325, 840, 856, 875, 990, 1260, 1365, 1405
TatI WGTACW 3 cut(s) 9, 250, 299
TauI GCSGC 1 cut(s) 1484
TfiI GAWTC 4 cut(s) 86, 953, 1253, 1569
Tru1I TTAA 4 cut(s) 809, 878, 1434, 1503
Tru9I TTAA 4 cut(s) 809, 878, 1434, 1503
TscAI CASTG 3 cut(s) 183, 552, 975
TseFI GTSAC 2 cut(s) 1354, 1555
TseI GCWGC 5 cut(s) 44, 1031, 1052, 1088, 1410
Tsp45I GTSAC 2 cut(s) 1354, 1555
TspDTI ATGAA 8 cut(s) 229, 348, 395, 429, 888, 912, 960, 1203
TspGWI ACGGA 3 cut(s) 1083, 1503, 1574
TspRI CASTG 3 cut(s) 183, 552, 975
VpaK11BI GGWCC 2 cut(s) 556, 1511
XagI CCTNNNNNAGG 1 cut(s) 1060
XapI RAATTY 4 cut(s) 98, 325, 840, 1260
XceI RCATGY 4 cut(s) 869, 1043, 1100, 1441
XcmI CCANNNNNNNNNTGG 1 cut(s) 1469
XhoI CTCGAG 1 cut(s) 320
XmiI GTMKAC 1 cut(s) 18
XmnI GAANNNNTTC 1 cut(s) 1170
XspI CTAG 6 cut(s) 431, 584, 1152, 1385, 1524, 1573
ZrmI AGTACT 1 cut(s) 11
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.