FvH4_3g22500

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003621
Physical Location & Seq
Forward (+)
15517615 .. 15520347
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22500.t3

Sequence Viewer

Length: 1062 bp
ATGCTCAAGCTTCTCTCCTCATCTCACAAAACCCCAACCCTAAACCCTCACAAACCCCTCCTCTTCCTCTCCCGCCTCCTTACCCAATTCTCCCACCTTCCCTCCGATTACGACCCGCCGTTTTCTCCAACTTCCAAACCCCAAACCCCCAAAAACATTACGACCCAGAATGCTGACCCGAAAAAGGACCCGACCCACCAGCCTCTCAAATCGGACCTTCCCTTCGACTTCAGCTACTCCTACTCCGAGATCCCGTCGGCGGAACCCATTTCCTTCCGGGAGTCGCCAAAGTTCTCTCCTTTCGGGCCGGGTCGGCTCGATAGGAAATGGACCGGGACGGCTGCCCCGGCCCAACAAGAACCGGACTTGAACCAGCTGGCGGAGCAGAGAAAACGGGTTCTCGGTGACCCGCTTACGGAGGAGGAGGTGGCTGAGCTGGTGGAACGGTACCGGCATAGCGATTGCTCCCGCCAAATTAATCTGGGGAAGGGAGGAGTTACTCATAACATGTTAGATGATATCCACAACCATTGGAAGCGAGCTGAGGCTGTGAGGATCAAGTGTTTGGGGGTGCCAACTCTTGATATGGACAATGTTTGCTTCCACCTTGAGGACAAGTCTGGAGGGGCGATTATTTACCGGCATATAAATGTTCTTATTTTATACCGCGGTCGAAATTATGATCCGAAAAATCGGCCAGTAATACCTGTGATGCTTTGGAAGCCGTATCCTCCGATATATCCAAAGCTTGTGAAGAATGTTGCTGATGGCTTGACATTTGAAGAGACGAAGGAGTTACGTAACAGAGGACTAAATTCCCTCCCTATCATGAAACTTACTAGAAATGGTGTGTATGTGAATGTTGTAGAAAGTGTAAAGGAGGCGTTCATGACTGAAGAGGTAGTGAGACTAGATTGCACTCATGTTGGTACCAGTGATTGCAAAAGAATAGGCGCGAAATTGAGGGACCTTGTACCTTGCATTCCCATTTTGTTCAAAGACGAGCAGATCATACTGTGGAGGGGGAAACAAGATCATGAACAAGACTCCCAAGTTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

354

Amino Acids

40.29

Weight (kDa)

8.59

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 138 - 207 7.2e-12 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 447, 929
AccB1I GGYRCC 3 cut(s) 447, 571, 929
AccII CGCG 2 cut(s) 669, 956
AciI CCGC 8 cut(s) 73, 116, 260, 380, 410, 469, 667, 669
AclWI GGATC 3 cut(s) 244, 563, 677
AcoI YGGCCR 1 cut(s) 695
AcsI RAATTY 1 cut(s) 814
AcuI CTGAAG 2 cut(s) 214, 915
AfaI GTAC 3 cut(s) 449, 931, 975
AfiI CCNNNNNNNGG 5 cut(s) 184, 259, 379, 415, 610
AflIII ACRYGT 1 cut(s) 507
AgsI TTSAA 3 cut(s) 370, 782, 997
AluBI AGCT 6 cut(s) 10, 234, 376, 436, 542, 748
AluI AGCT 6 cut(s) 10, 234, 376, 436, 542, 748
Alw26I GTCTC 2 cut(s) 779, 901
AlwI GGATC 3 cut(s) 244, 563, 677
AoxI GGCC 3 cut(s) 305, 348, 695
ApeKI GCWGC 1 cut(s) 341
ApoI RAATTY 1 cut(s) 814
ArsI GACNNNNNNTTYG 2 cut(s) 206, 238
AseI ATTAAT 1 cut(s) 477
Asp718I GGTACC 2 cut(s) 447, 929
AspLEI GCGC 1 cut(s) 956
AspS9I GGNCC 6 cut(s) 187, 214, 305, 330, 349, 967
AsuC2I CCSGG 4 cut(s) 278, 309, 334, 347
AsuHPI GGTGA 1 cut(s) 416
AvaII GGWCC 4 cut(s) 187, 214, 330, 967
BanI GGYRCC 3 cut(s) 447, 571, 929
BbvCI CCTCAGC 1 cut(s) 543
BbvI GCAGC 1 cut(s) 328
BccI CCATC 1 cut(s) 761
BceAI ACGGC 3 cut(s) 103, 354, 709
BciVI GTATCC 1 cut(s) 738
BcnI CCSGG 4 cut(s) 278, 309, 334, 347
BcoDI GTCTC 2 cut(s) 779, 901
BfaI CTAG 2 cut(s) 840, 911
BfuI GTATCC 1 cut(s) 738
BglI GCCNNNNNGGC 1 cut(s) 313
BisI GCNGC 1 cut(s) 342
BlpI GCTNAGC 1 cut(s) 432
BlsI GCNGC 1 cut(s) 343
Bme1390I CCNGG 4 cut(s) 278, 309, 334, 347
Bme18I GGWCC 4 cut(s) 187, 214, 330, 967
BmgT120I GGNCC 6 cut(s) 187, 214, 305, 330, 349, 967
BmiI GGNNCC 6 cut(s) 189, 264, 449, 573, 931, 968
BmrFI CCNGG 4 cut(s) 278, 309, 334, 347
BmsI GCATC 1 cut(s) 702
BpmI CTGGAG 1 cut(s) 642
Bpu10I CCTNAGC 1 cut(s) 543
Bpu1102I GCTNAGC 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 4 cut(s) 278, 309, 334, 347
BsaAI YACGTR 1 cut(s) 800
BsaJI CCNNGG 2 cut(s) 345, 667
BsaWI WCCGGW 1 cut(s) 361
BsaXI ACNNNNNCTCC 4 cut(s) 86, 116, 227, 257
Bsc4I CCNNNNNNNGG 5 cut(s) 184, 259, 379, 415, 610
Bse118I RCCGGY 2 cut(s) 450, 639
Bse1I ACTGG 2 cut(s) 698, 933
BseDI CCNNGG 2 cut(s) 345, 667
BseLI CCNNNNNNNGG 5 cut(s) 184, 259, 379, 415, 610
BseMII CTCAG 2 cut(s) 423, 534
BseNI ACTGG 2 cut(s) 698, 933
BseRI GAGGAG 5 cut(s) 7, 50, 434, 437, 507
BseXI GCAGC 1 cut(s) 328
Bsh1236I CGCG 2 cut(s) 669, 956
Bsh1285I CGRYCG 1 cut(s) 673
BshFI GGCC 3 cut(s) 307, 350, 697
BshNI GGYRCC 3 cut(s) 447, 571, 929
BsiEI CGRYCG 1 cut(s) 673
BsiSI CCGG 7 cut(s) 277, 308, 333, 347, 362, 451, 640
BslFI GGGAC 2 cut(s) 349, 980
BslI CCNNNNNNNGG 5 cut(s) 184, 259, 379, 415, 610
BsmAI GTCTC 2 cut(s) 779, 901
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 2 cut(s) 349, 980
BsmI GAATGC 2 cut(s) 175, 981
BsnI GGCC 3 cut(s) 307, 350, 697
Bsp143I GATC 5 cut(s) 249, 555, 682, 1008, 1033
Bsp1720I GCTNAGC 1 cut(s) 432
BspACI CCGC 8 cut(s) 73, 116, 260, 380, 410, 469, 667, 669
BspANI GGCC 3 cut(s) 307, 350, 697
BspCNI CTCAG 2 cut(s) 424, 535
BspFNI CGCG 2 cut(s) 669, 956
BspHI TCATGA 3 cut(s) 828, 888, 1036
BspLI GGNNCC 6 cut(s) 189, 264, 449, 573, 931, 968
BspPI GGATC 3 cut(s) 244, 563, 677
BspT107I GGYRCC 3 cut(s) 447, 571, 929
BsrFI RCCGGY 2 cut(s) 450, 639
BsrI ACTGG 2 cut(s) 698, 933
BssAI RCCGGY 2 cut(s) 450, 639
BssECI CCNNGG 2 cut(s) 345, 667
BssMI GATC 5 cut(s) 249, 555, 682, 1008, 1033
Bst4CI ACNGT 2 cut(s) 447, 1017
Bst6I CTCTTC 3 cut(s) 68, 777, 891
BstBAI YACGTR 1 cut(s) 800
BstC8I GCNNGC 2 cut(s) 378, 540
BstDEI CTNAG 2 cut(s) 432, 543
BstDSI CCRYGG 1 cut(s) 667
BstEII GGTNACC 1 cut(s) 404
BstFNI CGCG 2 cut(s) 669, 956
BstHHI GCGC 1 cut(s) 956
BstKTI GATC 5 cut(s) 252, 558, 685, 1011, 1036
BstMAI GTCTC 2 cut(s) 779, 901
BstMBI GATC 5 cut(s) 249, 555, 682, 1008, 1033
BstMCI CGRYCG 1 cut(s) 673
BstMWI GCNNNNNNNGC 4 cut(s) 313, 347, 382, 721
BstNSI RCATGY 1 cut(s) 511
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 4 cut(s) 276, 307, 332, 345
BstSNI TACGTA 1 cut(s) 800
BstUI CGCG 2 cut(s) 669, 956
BstV1I GCAGC 1 cut(s) 328
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BsuI GTATCC 1 cut(s) 738
BsuRI GGCC 3 cut(s) 307, 350, 697
BtgI CCRYGG 1 cut(s) 667
BtsIMutI CAGTG 1 cut(s) 940
Cac8I GCNNGC 2 cut(s) 378, 540
CciI TCATGA 3 cut(s) 828, 888, 1036
CfoI GCGC 1 cut(s) 956
Cfr10I RCCGGY 2 cut(s) 450, 639
Cfr13I GGNCC 6 cut(s) 187, 214, 305, 330, 349, 967
Cfr42I CCGCGG 1 cut(s) 670
Csp6I GTAC 3 cut(s) 448, 930, 974
CspCI CAANNNNNGTGG 2 cut(s) 512, 547
CviAII CATG 5 cut(s) 508, 829, 889, 923, 1037
CviQI GTAC 3 cut(s) 448, 930, 974
DdeI CTNAG 2 cut(s) 432, 543
DpnI GATC 5 cut(s) 251, 557, 684, 1010, 1035
DpnII GATC 5 cut(s) 249, 555, 682, 1008, 1033
EaeI YGGCCR 1 cut(s) 695
Eam1104I CTCTTC 3 cut(s) 68, 777, 891
EarI CTCTTC 3 cut(s) 68, 777, 891
EciI GGCGGA 2 cut(s) 275, 395
Eco105I TACGTA 1 cut(s) 800
Eco32I GATATC 1 cut(s) 520
Eco47I GGWCC 4 cut(s) 187, 214, 330, 967
Eco57I CTGAAG 2 cut(s) 214, 915
Eco91I GGTNACC 1 cut(s) 404
EcoO109I RGGNCCY 2 cut(s) 187, 967
EcoO65I GGTNACC 1 cut(s) 404
EcoRV GATATC 1 cut(s) 520
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 5 cut(s) 511, 832, 892, 926, 1040
FaqI GGGAC 2 cut(s) 349, 980
FatI CATG 5 cut(s) 507, 828, 888, 922, 1036
FauI CCCGC 4 cut(s) 80, 123, 417, 476
Fnu4HI GCNGC 1 cut(s) 342
Fsp4HI GCNGC 1 cut(s) 342
FspBI CTAG 2 cut(s) 840, 911
GlaI GCGC 1 cut(s) 955
GluI GCNGC 1 cut(s) 342
GsuI CTGGAG 1 cut(s) 642
HaeIII GGCC 3 cut(s) 307, 350, 697
HapII CCGG 7 cut(s) 277, 308, 333, 347, 362, 451, 640
HhaI GCGC 1 cut(s) 956
Hin1II CATG 5 cut(s) 511, 832, 892, 926, 1040
Hin6I GCGC 1 cut(s) 954
HinP1I GCGC 1 cut(s) 954
HindIII AAGCTT 2 cut(s) 8, 746
HinfI GANTC 2 cut(s) 281, 1046
HpaII CCGG 7 cut(s) 277, 308, 333, 347, 362, 451, 640
HphI GGTGA 1 cut(s) 416
Hpy188I TCNGA 5 cut(s) 106, 214, 247, 687, 735
Hpy188III TCNNGA 5 cut(s) 581, 621, 829, 889, 1037
Hpy99I CGWCG 1 cut(s) 259
HpyAV CCTTC 6 cut(s) 107, 227, 232, 283, 481, 784
HpyCH4III ACNGT 2 cut(s) 447, 1017
HpyCH4IV ACGT 1 cut(s) 799
HpyCH4V TGCA 3 cut(s) 918, 942, 981
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 347, 382, 721
HpyF3I CTNAG 2 cut(s) 432, 543
HpySE526I ACGT 1 cut(s) 799
Hsp92II CATG 5 cut(s) 511, 832, 892, 926, 1040
HspAI GCGC 1 cut(s) 954
KpnI GGTACC 2 cut(s) 451, 933
KspI CCGCGG 1 cut(s) 670
Kzo9I GATC 5 cut(s) 249, 555, 682, 1008, 1033
LmnI GCTCC 2 cut(s) 382, 470
Lsp1109I GCAGC 1 cut(s) 328
LweI GCATC 1 cut(s) 702
MaeI CTAG 2 cut(s) 840, 911
MaeII ACGT 1 cut(s) 799
MaeIII GTNAC 4 cut(s) 404, 496, 795, 800
MalI GATC 5 cut(s) 251, 557, 684, 1010, 1035
MboI GATC 5 cut(s) 249, 555, 682, 1008, 1033
MboII GAAGA 4 cut(s) 55, 766, 794, 908
MflI RGATCY 1 cut(s) 249
MluCI AATT 5 cut(s) 86, 474, 676, 814, 959
MlyI GAGTC 2 cut(s) 290, 1040
MmeI TCCRAC 1 cut(s) 152
MseI TTAA 1 cut(s) 477
MslI CAYNNNNRTG 1 cut(s) 648
MspA1I CMGCKG 2 cut(s) 376, 669
MspI CCGG 7 cut(s) 277, 308, 333, 347, 362, 451, 640
MspR9I CCNGG 4 cut(s) 278, 309, 334, 347
Mva1269I GAATGC 2 cut(s) 175, 981
MvnI CGCG 2 cut(s) 669, 956
MwoI GCNNNNNNNGC 4 cut(s) 313, 347, 382, 721
NciI CCSGG 4 cut(s) 278, 309, 334, 347
NdeII GATC 5 cut(s) 249, 555, 682, 1008, 1033
NlaIII CATG 5 cut(s) 511, 832, 892, 926, 1040
NlaIV GGNNCC 6 cut(s) 189, 264, 449, 573, 931, 968
NmuCI GTSAC 1 cut(s) 404
NspI RCATGY 1 cut(s) 511
PagI TCATGA 3 cut(s) 828, 888, 1036
PciI ACATGT 1 cut(s) 507
PctI GAATGC 2 cut(s) 175, 981
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 1 cut(s) 343
PleI GAGTC 2 cut(s) 289, 1040
PpsI GAGTC 2 cut(s) 289, 1040
Ppu21I YACGTR 1 cut(s) 800
PpuMI RGGWCCY 2 cut(s) 187, 967
PscI ACATGT 1 cut(s) 507
PshBI ATTAAT 1 cut(s) 477
Psp5II RGGWCCY 2 cut(s) 187, 967
PspEI GGTNACC 1 cut(s) 404
PspN4I GGNNCC 6 cut(s) 189, 264, 449, 573, 931, 968
PspPI GGNCC 6 cut(s) 187, 214, 305, 330, 349, 967
PspPPI RGGWCCY 2 cut(s) 187, 967
PsuI RGATCY 1 cut(s) 249
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 3 cut(s) 449, 931, 975
RsaNI GTAC 3 cut(s) 448, 930, 974
RseI CAYNNNNRTG 1 cut(s) 648
SacII CCGCGG 1 cut(s) 670
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 342
Sau3AI GATC 5 cut(s) 249, 555, 682, 1008, 1033
Sau96I GGNCC 6 cut(s) 187, 214, 305, 330, 349, 967
SchI GAGTC 2 cut(s) 290, 1040
ScrFI CCNGG 4 cut(s) 278, 309, 334, 347
SfaNI GCATC 1 cut(s) 702
Sfr303I CCGCGG 1 cut(s) 670
SgrBI CCGCGG 1 cut(s) 670
SinI GGWCC 4 cut(s) 187, 214, 330, 967
SmiMI CAYNNNNRTG 1 cut(s) 648
SmlI CTYRAG 2 cut(s) 5, 608
SmoI CTYRAG 2 cut(s) 5, 608
SnaBI TACGTA 1 cut(s) 800
Sse9I AATT 5 cut(s) 86, 474, 676, 814, 959
SsiI CCGC 8 cut(s) 73, 116, 260, 380, 410, 469, 667, 669
SspMI CTAG 2 cut(s) 840, 911
StyD4I CCNGG 4 cut(s) 276, 307, 332, 345
TaaI ACNGT 2 cut(s) 447, 1017
TaiI ACGT 1 cut(s) 802
TaqI TCGA 3 cut(s) 225, 318, 673
TasI AATT 5 cut(s) 86, 474, 676, 814, 959
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TscAI CASTG 1 cut(s) 940
TseFI GTSAC 1 cut(s) 404
TseI GCWGC 1 cut(s) 341
Tsp45I GTSAC 1 cut(s) 404
TspDTI ATGAA 3 cut(s) 845, 877, 1053
TspGWI ACGGA 1 cut(s) 431
TspRI CASTG 1 cut(s) 940
VpaK11BI GGWCC 4 cut(s) 187, 214, 330, 967
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 1 cut(s) 814
XceI RCATGY 1 cut(s) 511
XspI CTAG 2 cut(s) 840, 911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.