Rroxscaffold_1G00040420

CRS2-associated factor 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0003621
Physical Location & Seq
Forward (+)
58106288 .. 58108671
2384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040420.1

Sequence Viewer

Length: 1194 bp
ATGTGGCTCTGGCGCCAATTACGATTTTACCCCTCCTCAAGTTCATCTCAGCTTTCACTTTCAAAAATCCGAGTCCATATGTTAAGCAAACCAAACCAAACCAAACTAAACACTCTGTTCCAAATGCTCAAGCTTCTCTCCTCAGCTCATAAAACCCAAACCCAAACCCTAAACCCTAACAAACCATTCCTCTTCCTCTCTCGCCTCCTCAGCCAATCTCTCTCTAGCAATCCCTACGATTACGACCCTCCGTTTTCTCCAGCTTCCAAACCCCAAAACCCCAAAAACAAAAAGACCCAGAATGCTGACCCGAACAAAGACCCGACCACCCAGCCTCTCAAATCGGACCTTCCCTTCGACTTCAGCTACTCCTACTCGGAGACCCCGTCGGCCGAACCCATTTCTTTCCGGGAGTCCCCAAAGTTCTCTCCTTTCGGGCCGGGACGGCTTGATAGGAAATGGACCGGGACGGTCGCCCCGGCCCAACAGGAAGTGGACTTGAACCGGCTGGCCCAGGAGAGAAAACGGGTTCTCGGAGACCCGCTTTCGGAGGAGGAAGTGGCTGAGCTGGTGGAACGGTATCGGCATAGTGATTGCTCTCGCCAAATCAATTTGGGAAAGGGAGGAGTTACTCATAACATGTTAGATGACATCCACAACCATTGGAAGCGGGCTGAAGCTGTGAGGATCAAGTGTTTGGGGGTGCCAACTCTTGATATGGACAATGTTTGCTTCCACCTTGAGGACAAGTCTGGAGGGGCAATTATCTACCGGCATATTAATGTCCTTATTTTATACCGCGGTCGAAATTATGATCCGAACAATCGGCCAGTAATACCTGTAATGCTCTGGAAGCCCTATCCTCCGATTTATCCAAAGCTTGTGAAGAATGTTGCTGATGGCTTGACATTTGAAGAGACGAAGGAATTACGAAACAGAGGACTAAATTCCTTCCCTCTCATGAAACTTACTAGAAATGGTGTGTATGTGAATGTTGTGGACAGAGTAAAGGAGGCCTTCAAGACTGAAGAGGTAGTGAGACTAGACTGCACCCATGTTGGTACCAGTGATTGCAAAAGAATAGGTGCAAAATTGAGGGACCTTGCACCTTGCATCCCCCTTTTGTTCAAAGATGAGCAGATTATACTGTGGAGGGGGGAAACAAGATCAAGAACAAGACTCCAAGTGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRS1_YhbY

Protein Analysis

397

Amino Acids

45.64

Weight (kDa)

9.62

Isoelectric Point (pI)

47.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRS1_YhbY PF01985 182 - 267 2.6e-17 CRS1 / YhbY (CRM) domain
CRS1_YhbY PF01985 302 - 385 2.4e-12 CRS1 / YhbY (CRM) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1061
AccB1I GGYRCC 3 cut(s) 12, 703, 1061
AccII CGCG 1 cut(s) 801
AciI CCGC 4 cut(s) 542, 670, 799, 801
AclWI GGATC 2 cut(s) 695, 809
AcoI YGGCCR 2 cut(s) 390, 827
AcsI RAATTY 1 cut(s) 946
AcuI CTGAAG 3 cut(s) 346, 696, 1047
AcyI GRCGYC 1 cut(s) 13
AfaI GTAC 1 cut(s) 1063
AfiI CCNNNNNNNGG 2 cut(s) 547, 742
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 5 cut(s) 63, 502, 914, 1021, 1129
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 8 cut(s) 52, 133, 146, 263, 366, 568, 680, 880
AluI AGCT 8 cut(s) 52, 133, 146, 263, 366, 568, 680, 880
Alw26I GTCTC 4 cut(s) 374, 531, 911, 1033
AlwI GGATC 2 cut(s) 695, 809
AoxI GGCC 6 cut(s) 390, 437, 480, 510, 827, 1014
ApoI RAATTY 1 cut(s) 946
ArsI GACNNNNNNTTYG 2 cut(s) 338, 370
AseI ATTAAT 1 cut(s) 780
Asp718I GGTACC 1 cut(s) 1061
AspLEI GCGC 1 cut(s) 15
AspS9I GGNCC 6 cut(s) 346, 437, 462, 481, 511, 1099
AsuC2I CCSGG 4 cut(s) 410, 441, 466, 479
AvaII GGWCC 3 cut(s) 346, 462, 1099
BanI GGYRCC 3 cut(s) 12, 703, 1061
BbvCI CCTCAGC 2 cut(s) 142, 209
BccI CCATC 1 cut(s) 893
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 1 cut(s) 515
BcnI CCSGG 4 cut(s) 410, 441, 466, 479
BcoDI GTCTC 4 cut(s) 374, 531, 911, 1033
BfaI CTAG 3 cut(s) 225, 972, 1043
BfoI RGCGCY 1 cut(s) 16
BglI GCCNNNNNGGC 1 cut(s) 445
BlpI GCTNAGC 1 cut(s) 564
Bme1390I CCNGG 5 cut(s) 410, 441, 466, 479, 515
Bme18I GGWCC 3 cut(s) 346, 462, 1099
BmgT120I GGNCC 6 cut(s) 346, 437, 462, 481, 511, 1099
BmiI GGNNCC 4 cut(s) 14, 705, 1063, 1100
BmrFI CCNGG 5 cut(s) 410, 441, 466, 479, 515
BmsI GCATC 1 cut(s) 1122
BpmI CTGGAG 2 cut(s) 243, 774
Bpu10I CCTNAGC 2 cut(s) 142, 209
Bpu1102I GCTNAGC 1 cut(s) 564
BpuEI CTTGAG 3 cut(s) 22, 113, 761
BpuMI CCSGG 4 cut(s) 410, 441, 466, 479
BsaHI GRCGYC 1 cut(s) 13
BsaI GGTCTC 2 cut(s) 374, 531
BsaJI CCNNGG 3 cut(s) 477, 513, 799
BsaXI ACNNNNNCTCC 2 cut(s) 371, 401
Bsc4I CCNNNNNNNGG 2 cut(s) 547, 742
Bse118I RCCGGY 2 cut(s) 504, 771
Bse1I ACTGG 2 cut(s) 830, 1065
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 3 cut(s) 477, 513, 799
BseGI GGATG 2 cut(s) 651, 1113
BseLI CCNNNNNNNGG 2 cut(s) 547, 742
BseMII CTCAG 4 cut(s) 62, 156, 223, 555
BseNI ACTGG 2 cut(s) 830, 1065
BseRI GAGGAG 5 cut(s) 25, 130, 197, 566, 639
BseX3I CGGCCG 1 cut(s) 390
BseYI CCCAGC 1 cut(s) 330
BsgI GTGCAG 1 cut(s) 1033
Bsh1236I CGCG 1 cut(s) 801
Bsh1285I CGRYCG 3 cut(s) 393, 474, 805
BshFI GGCC 6 cut(s) 392, 439, 482, 512, 829, 1016
BshNI GGYRCC 3 cut(s) 12, 703, 1061
BsiEI CGRYCG 3 cut(s) 393, 474, 805
BsiSI CCGG 6 cut(s) 409, 440, 465, 479, 505, 772
BslFI GGGAC 4 cut(s) 400, 456, 481, 1112
BslI CCNNNNNNNGG 2 cut(s) 547, 742
BsmAI GTCTC 4 cut(s) 374, 531, 911, 1033
BsmBI CGTCTC 1 cut(s) 911
BsmFI GGGAC 4 cut(s) 400, 456, 481, 1112
BsmI GAATGC 1 cut(s) 307
BsnI GGCC 6 cut(s) 392, 439, 482, 512, 829, 1016
Bso31I GGTCTC 2 cut(s) 374, 531
Bsp143I GATC 3 cut(s) 687, 814, 1166
Bsp1720I GCTNAGC 1 cut(s) 564
BspACI CCGC 4 cut(s) 542, 670, 799, 801
BspANI GGCC 6 cut(s) 392, 439, 482, 512, 829, 1016
BspCNI CTCAG 4 cut(s) 61, 155, 222, 556
BspFNI CGCG 1 cut(s) 801
BspHI TCATGA 1 cut(s) 960
BspLI GGNNCC 4 cut(s) 14, 705, 1063, 1100
BspPI GGATC 2 cut(s) 695, 809
BspT107I GGYRCC 3 cut(s) 12, 703, 1061
BspTNI GGTCTC 2 cut(s) 374, 531
BsrFI RCCGGY 2 cut(s) 504, 771
BsrI ACTGG 2 cut(s) 830, 1065
BssAI RCCGGY 2 cut(s) 504, 771
BssECI CCNNGG 3 cut(s) 477, 513, 799
BssMI GATC 3 cut(s) 687, 814, 1166
BssNI GRCGYC 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 3 cut(s) 472, 579, 1149
Bst6I CTCTTC 3 cut(s) 197, 909, 1023
BstACI GRCGYC 1 cut(s) 13
BstC8I GCNNGC 2 cut(s) 510, 672
BstDEI CTNAG 4 cut(s) 48, 142, 209, 564
BstDSI CCRYGG 1 cut(s) 799
BstF5I GGATG 2 cut(s) 651, 1113
BstFNI CGCG 1 cut(s) 801
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 3 cut(s) 690, 817, 1169
BstMAI GTCTC 4 cut(s) 374, 531, 911, 1033
BstMBI GATC 3 cut(s) 687, 814, 1166
BstMCI CGRYCG 3 cut(s) 393, 474, 805
BstMWI GCNNNNNNNGC 3 cut(s) 210, 445, 853
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 1 cut(s) 643
BstSCI CCNGG 5 cut(s) 408, 439, 464, 477, 513
BstUI CGCG 1 cut(s) 801
BstZI CGGCCG 1 cut(s) 390
BsuRI GGCC 6 cut(s) 392, 439, 482, 512, 829, 1016
BtgI CCRYGG 1 cut(s) 799
BtsCI GGATG 2 cut(s) 651, 1113
BtsIMutI CAGTG 1 cut(s) 1072
Cac8I GCNNGC 2 cut(s) 510, 672
CciI TCATGA 1 cut(s) 960
CfoI GCGC 1 cut(s) 15
Cfr10I RCCGGY 2 cut(s) 504, 771
Cfr13I GGNCC 6 cut(s) 346, 437, 462, 481, 511, 1099
Cfr42I CCGCGG 1 cut(s) 802
Csp6I GTAC 1 cut(s) 1062
CspCI CAANNNNNGTGG 2 cut(s) 644, 679
CviAII CATG 3 cut(s) 640, 961, 1055
CviQI GTAC 1 cut(s) 1062
DdeI CTNAG 4 cut(s) 48, 142, 209, 564
DinI GGCGCC 1 cut(s) 14
DpnI GATC 3 cut(s) 689, 816, 1168
DpnII GATC 3 cut(s) 687, 814, 1166
EaeI YGGCCR 2 cut(s) 390, 827
EagI CGGCCG 1 cut(s) 390
Eam1104I CTCTTC 3 cut(s) 197, 909, 1023
EarI CTCTTC 3 cut(s) 197, 909, 1023
EclXI CGGCCG 1 cut(s) 390
Eco147I AGGCCT 1 cut(s) 1016
Eco31I GGTCTC 2 cut(s) 374, 531
Eco47I GGWCC 3 cut(s) 346, 462, 1099
Eco52I CGGCCG 1 cut(s) 390
Eco57I CTGAAG 3 cut(s) 346, 696, 1047
EcoO109I RGGNCCY 1 cut(s) 1099
EcoRII CCWGG 1 cut(s) 513
EgeI GGCGCC 1 cut(s) 14
EheI GGCGCC 1 cut(s) 14
Esp3I CGTCTC 1 cut(s) 911
FaeI CATG 3 cut(s) 643, 964, 1058
FalI AAGNNNNNCTT 2 cut(s) 1001, 1033
FaqI GGGAC 4 cut(s) 400, 456, 481, 1112
FatI CATG 3 cut(s) 639, 960, 1054
FauI CCCGC 2 cut(s) 549, 663
FauNDI CATATG 1 cut(s) 78
FokI GGATG 2 cut(s) 638, 1100
FspBI CTAG 3 cut(s) 225, 972, 1043
GlaI GCGC 1 cut(s) 14
GsaI CCCAGC 1 cut(s) 334
GsuI CTGGAG 2 cut(s) 243, 774
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 6 cut(s) 392, 439, 482, 512, 829, 1016
HapII CCGG 6 cut(s) 409, 440, 465, 479, 505, 772
HhaI GCGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 13
Hin1II CATG 3 cut(s) 643, 964, 1058
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HindIII AAGCTT 2 cut(s) 131, 878
HinfI GANTC 3 cut(s) 72, 413, 1179
HpaII CCGG 6 cut(s) 409, 440, 465, 479, 505, 772
Hpy166II GTNNAC 2 cut(s) 496, 1000
Hpy188I TCNGA 7 cut(s) 71, 346, 379, 536, 550, 819, 867
Hpy188III TCNNGA 6 cut(s) 713, 753, 850, 961, 1021, 1170
Hpy8I GTNNAC 2 cut(s) 496, 1000
Hpy99I CGWCG 1 cut(s) 391
HpyAV CCTTC 5 cut(s) 359, 364, 916, 961, 1027
HpyCH4III ACNGT 3 cut(s) 472, 579, 1149
HpyCH4V TGCA 5 cut(s) 1050, 1074, 1088, 1106, 1113
HpyF10VI GCNNNNNNNGC 3 cut(s) 210, 445, 853
HpyF3I CTNAG 4 cut(s) 48, 142, 209, 564
Hsp92I GRCGYC 1 cut(s) 13
Hsp92II CATG 3 cut(s) 643, 964, 1058
HspAI GCGC 1 cut(s) 13
KasI GGCGCC 1 cut(s) 12
KpnI GGTACC 1 cut(s) 1065
KspI CCGCGG 1 cut(s) 802
Kzo9I GATC 3 cut(s) 687, 814, 1166
LweI GCATC 1 cut(s) 1122
MaeI CTAG 3 cut(s) 225, 972, 1043
MaeIII GTNAC 1 cut(s) 628
MalI GATC 3 cut(s) 689, 816, 1168
MboI GATC 3 cut(s) 687, 814, 1166
MboII GAAGA 4 cut(s) 184, 898, 926, 1040
MluCI AATT 7 cut(s) 17, 610, 762, 808, 926, 946, 1091
Mly113I GGCGCC 1 cut(s) 13
MlyI GAGTC 3 cut(s) 81, 422, 1173
MseI TTAA 2 cut(s) 83, 780
MslI CAYNNNNRTG 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 801
MspI CCGG 6 cut(s) 409, 440, 465, 479, 505, 772
MspR9I CCNGG 5 cut(s) 410, 441, 466, 479, 515
Mva1269I GAATGC 1 cut(s) 307
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 801
MwoI GCNNNNNNNGC 3 cut(s) 210, 445, 853
NarI GGCGCC 1 cut(s) 13
NciI CCSGG 4 cut(s) 410, 441, 466, 479
NdeI CATATG 1 cut(s) 78
NdeII GATC 3 cut(s) 687, 814, 1166
NlaIII CATG 3 cut(s) 643, 964, 1058
NlaIV GGNNCC 4 cut(s) 14, 705, 1063, 1100
NspI RCATGY 1 cut(s) 643
PagI TCATGA 1 cut(s) 960
PceI AGGCCT 1 cut(s) 1016
PciI ACATGT 1 cut(s) 639
PcsI WCGNNNNNNNCGW 1 cut(s) 383
PctI GAATGC 1 cut(s) 307
PflFI GACNNNGTC 1 cut(s) 385
PfoI TCCNGGA 1 cut(s) 408
PleI GAGTC 3 cut(s) 80, 421, 1173
PluTI GGCGCC 1 cut(s) 16
PpsI GAGTC 3 cut(s) 80, 421, 1173
PpuMI RGGWCCY 1 cut(s) 1099
PscI ACATGT 1 cut(s) 639
PshBI ATTAAT 1 cut(s) 780
Psp5II RGGWCCY 1 cut(s) 1099
Psp6I CCWGG 1 cut(s) 513
PspFI CCCAGC 1 cut(s) 330
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 4 cut(s) 14, 705, 1063, 1100
PspPI GGNCC 6 cut(s) 346, 437, 462, 481, 511, 1099
PspPPI RGGWCCY 1 cut(s) 1099
PsyI GACNNNGTC 1 cut(s) 385
RsaI GTAC 1 cut(s) 1063
RsaNI GTAC 1 cut(s) 1062
RseI CAYNNNNRTG 1 cut(s) 780
SacII CCGCGG 1 cut(s) 802
SaqAI TTAA 2 cut(s) 83, 780
Sau3AI GATC 3 cut(s) 687, 814, 1166
Sau96I GGNCC 6 cut(s) 346, 437, 462, 481, 511, 1099
SchI GAGTC 3 cut(s) 81, 422, 1173
ScrFI CCNGG 5 cut(s) 410, 441, 466, 479, 515
SfaNI GCATC 1 cut(s) 1122
SfoI GGCGCC 1 cut(s) 14
Sfr303I CCGCGG 1 cut(s) 802
SgrBI CCGCGG 1 cut(s) 802
SinI GGWCC 3 cut(s) 346, 462, 1099
SmiMI CAYNNNNRTG 1 cut(s) 780
SmlI CTYRAG 3 cut(s) 37, 128, 740
SmoI CTYRAG 3 cut(s) 37, 128, 740
Sse9I AATT 7 cut(s) 17, 610, 762, 808, 926, 946, 1091
SseBI AGGCCT 1 cut(s) 1016
SsiI CCGC 4 cut(s) 542, 670, 799, 801
SspDI GGCGCC 1 cut(s) 12
SspMI CTAG 3 cut(s) 225, 972, 1043
StuI AGGCCT 1 cut(s) 1016
StyD4I CCNGG 5 cut(s) 408, 439, 464, 477, 513
TaaI ACNGT 3 cut(s) 472, 579, 1149
TaqI TCGA 2 cut(s) 357, 805
TasI AATT 7 cut(s) 17, 610, 762, 808, 926, 946, 1091
Tru1I TTAA 2 cut(s) 83, 780
Tru9I TTAA 2 cut(s) 83, 780
TscAI CASTG 1 cut(s) 1072
TspDTI ATGAA 2 cut(s) 33, 977
TspGWI ACGGA 1 cut(s) 240
TspRI CASTG 1 cut(s) 1072
Tth111I GACNNNGTC 1 cut(s) 385
VpaK11BI GGWCC 3 cut(s) 346, 462, 1099
VspI ATTAAT 1 cut(s) 780
XapI RAATTY 1 cut(s) 946
XceI RCATGY 1 cut(s) 643
XspI CTAG 3 cut(s) 225, 972, 1043
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.