FvH4_3g25260

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0016595
Physical Location & Seq
Reverse (-)
18206647 .. 18212439
5793 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g25260.t1

Sequence Viewer

Length: 3225 bp
ATGAGTGGGAGTGGGTGTCTCAGGTCCTTGATGGTCACTCTAAACCAATGCCACAAGCCCATGTTCTTGCATCGCTACCGCCTTGATTTCAGAACCAAACCCACTTCAGTTATCAATCTTGGCAAGCACTTTCGTGGGAGTGTGATTAGTTTGAGCTTGGCGGAGTATAGTAATGCAGCCAATGCCCATGTCTTGGATGAGGATATAAATCATCAAAATGAAGGTGGTCCAAAGGGGATTGATTTATTGCATTCTATGGAATCACGTTGCATTCGTGCTAATTCTCAGACATATATTTGGCTTTTGAAAGGGTGCTTGAGTTCTGGGTCTCTGTTAGAGGCTAGGAATCTTCACTCTAGAGTATTGAAGTTGGGTTTTGGTGGAGATATTGAAATAAGTAACTTGTTTGTTGGTGTTTACCTTGCAAATGGTGATGCCTGCAGCGCAGTTAAGGTTTTTGATGATCTACCATATAGAAGTTTGTTTTCATGGAATAACATAATTCATGGGTTTCTTGCAAAGAAGTTGACTGGTCAAGTATTAGGTTTCTTTTCGCAAATGATGGCAGAAAATGTGTATCCTGATGAGACTACCTTTGCAGGAGTTTTAAGGGCATGTGGTGGTGGTAATGCCAGTATTCAATATGTGGAACAGATTCATGCTAGGATTATCCGTCATGGTTTTGCTACTAGATTACTTGTATGTAATCCTTTGATCGATTTGTATGCCAAGAACGGCTCTGTGGATTCTGCTAAAAAAGTCTTTGATAGATTGTGTTTTAGGGATAGTGTTTCCTGGGTGGCTATAATTTCTGGCTTATCTCGGAATGGACTCGAAGAAGAAGCTGTTCTCTTATTCATCCAGATGTATACTTCAGGAATTTTCCCAACTCCTTATGTTTTCTCGAGCGTGATTAGTGCCTGTGCAAAAATAGAGTTATTTGAGCTGGGTGAGCAGCTGCAATGCCTTGTTCTCAAGGGAGGCTTTTCTTTTGAAACGTACGTATGCAATGCTCTGGTAACATTATATTCCCGTTCAGGGAACTTCATATCTGCTGAACAAGTTTTCAATACAATGTGGTACAGGGATGGTGTATCATATAACTCATTGATATCTGGGTTAGCTCAGTGTGGATTTAGTGATAGAGCTTTGAAATTGTTCAAGAAAATGCAGAGTGAATGTATGGAACCTGACTGTGTAACAATTGCTAGTCTTTTGAGTGCGTGTGCATCACTTGGTTATCTGTATAAGGGGAAACAACTGCACTCATATGCAATTAAAGCCGGAATGTCATCAGATATTATCCTCGAAGGGGCTCTGCTTGATCTTTATGTGAAATGCTCAGATTTGCAGACTGCCTATGAATTTTTTCTTACAACAGAGACAGAAAATGTTGTCCTGTGGAATGTGATGCTAGTTGCTTATGGGCTGCTAGATGACCTGCAAGAATCGTTTCACATTTTTAAACAAATGCATGTTGAGGGTATGATTCCTAATCAATATACTTACCCGAGTATTTTGAGAACCTGCACTTCTGTTGGAGCTCTTAATTTAGGAGAACAAGTTCATACTCAAGCCATAAAAACTGGTTTTCAGTTTAATGCATATGTCTGTAGCGTGCTCATTGATATGTATGCTAAGCATGGAAAACTGGATACTGCCCTTGGAATACTCAGAAGACTCACTGAAGATGATGCTGTCTCGTGGACAGCTATGATTGCAGGGTATGCACAGCATGATTTATTTGCTGAAGCTCTTTTACTTTTTGAAGAAATGCTAAACCGAGGAATTCGATCTGACACCATAGTTTTGTCAAGTGCAATTAGTTCATGTGCTGGAATTCAAGCACTCAATCAAGGTAGACAGATTCATGCTCAGTCATGCATCTCTGGTTATTCGAATGATCTTTCTGTTGGCAATGCACTTGTTACCCTTTATGCTAGATGTGGTAGAATATGGGAAGCCTACCAAGCATTTGAGAAAATTGATACAAAAGATAATATATCATGGAATGGATTGATATCAGGGTTTGGACAGAGTGGGTACTGTGAGGAAGCACTGCAGGTATTTTCTCAGATGCATCGAGCTGGAGTAGAAGCTAATTTGTTCACATTTGGCTCTGCAGTCAGCGCTGCTGCTAATTTAGCAAATATAAAACAAGGAGAGCAGATTCATGCCTTGGTGATAAAAACTGGGAACAATTCAGAAGCTGAGGTTTCCAATGCTTTGATCACATTATACTCGAAGTGTGGCAGCGTTGATGACGCGAAGAGAGAGTTTATTGAAATGCCTGTTAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAGCATGGGCATGGTATTGAAGCATTACATCTATTTGAGCAGATGAAACAACTTGGTGTGGTGCCAAGCCATGTCACATTTGTGGGAGTACTTTCAGCTTGTAGCCATGTAGGCTTGATAAGTGAAGGGCTTGCATACTTTGAATCCATGAGCAAAGAGCATGGACTAGTTCCTAAGCCTGAGCATTATGCTTGTGTTGTGGATCTTCTTAGTCGTGCTGGTTCTTTGAACTGTGCAAGAAAATTCATAACCGAGATGCCAATAAAACCAGATTCAACAATTTGGAGAACTCTCTTAAGTGCTTGTATTGCTAAGAAGAACACAGAAATTGGGGAGGTTGCTGCCCGTCATCTTCTAAAACTAGAACCTGAAGACTCAGCAACTTACGTTCTCATATCAAACATGTATGCAGTGGCTGGATTGTGGGGTTATAGGGATCAGGCAAGGCAATTAATGAAGGAAAGGGGTGTGAAGAAAGAGCCTGGCCGCAGTTGGATTGAGGTAAAGAATTCTGTTCATGCATTCTATGTTGGCGACCGGCTGCATCCACTTGCCAATAAGATATATGAATTCTTAGGGGATTTGAATGAGCGGGCAGCCGAAATTGGCTATGTGGAGGACCGTAACAACCTTTGGAATGACATGGAACAACAGCATAAAGATCCAACAGTGTACATTCATAGTGAGAAACTGGCAATTACTTTTGGCCTGATTAGCCTATCTAGTACGATTCCGATACGTGTAATTAAGAATCTTCGTGTTTGCAATGATTGCCATAATTGGATCAAGCATACGTCAAAGATCTCAAAAAGAACTATCATAGTACGAGATGCTTATCGATTTCATCATTTCAAAGATGGAGTTTGCTCCTGTAAAGATTACTGGTAA

Protein Analysis

1075

Amino Acids

119.44

Weight (kDa)

6.59

Isoelectric Point (pI)

37.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 206 2.3e-06 PPR repeat family
PPR_2 PF13041 262 - 310 5.5e-07 PPR repeat family
PPR_2 PF13041 363 - 410 4.5e-09 PPR repeat family
PPR_1 PF12854 363 - 391 8.5e-06 PPR repeat
PPR PF01535 365 - 393 7.3e-08 PPR repeat
PPR_2 PF13041 464 - 510 8.9e-07 PPR repeat family
PPR_2 PF13041 565 - 612 1.5e-07 PPR repeat family
PPR PF01535 567 - 597 1.6e-07 PPR repeat
PPR_2 PF13041 666 - 710 8.9e-08 PPR repeat family
PPR PF01535 668 - 698 2.3e-07 PPR repeat
PPR_2 PF13041 767 - 814 4.6e-11 PPR repeat family
PPR PF01535 769 - 799 2.8e-08 PPR repeat
E_motif PF20431 886 - 946 1.2e-17 E motif
DYW_deaminase PF14432 982 - 1074 1.7e-33 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13650
fragaria_vesca FvH4_3g25260
malus_domestica MD12G1002600.v1.1
prunus_persica Prupe.7G002600_v2.0.a1
pyrus_communis pycom12g00230
rosa_chinensis RchiOBHm_Chr5g0046881
rosa_laevigata RLG00000034452
rosa_roxburghii Rroxscaffold_1G00034510
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana Rw5G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 1449, 1535, 2053
AccB1I GGYRCC 1 cut(s) 2398
AccB7I CCANNNNNTGG 1 cut(s) 193
AccBSI CCGCTC 1 cut(s) 2929
AccI GTMKAC 2 cut(s) 869, 1861
AccII CGCG 1 cut(s) 2267
AciI CCGC 4 cut(s) 79, 161, 2824, 2929
AclWI GGATC 4 cut(s) 2547, 2781, 2993, 3128
AcoI YGGCCR 1 cut(s) 2821
AcsI RAATTY 7 cut(s) 879, 1364, 1788, 1839, 2579, 2845, 2906
AcuI CTGAAG 5 cut(s) 90, 858, 1707, 1770, 2727
AfaI GTAC 7 cut(s) 1001, 1082, 2045, 2427, 3011, 3064, 3162
AfeI AGCGCT 1 cut(s) 2131
AfiI CCNNNNNNNGG 3 cut(s) 193, 1038, 1312
AflII CTTAAG 1 cut(s) 2632
AflIII ACRYGT 2 cut(s) 2739, 3076
AhlI ACTAGT 1 cut(s) 2503
AjnI CCWGG 2 cut(s) 794, 2818
AleI CACNNNNGTG 2 cut(s) 132, 2417
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 2 cut(s) 1546, 1623
Alw26I GTCTC 5 cut(s) 23, 333, 581, 1376, 1705
AlwI GGATC 4 cut(s) 2547, 2781, 2993, 3128
AlwNI CAGNNNCTG 2 cut(s) 2210, 2753
Ama87I CYCGRG 2 cut(s) 904, 1510
Aor51HI AGCGCT 1 cut(s) 2131
AoxI GGCC 2 cut(s) 2821, 3043
ApoI RAATTY 7 cut(s) 879, 1364, 1788, 1839, 2579, 2845, 2906
AseI ATTAAT 1 cut(s) 2789
Asp700I GAANNNNTTC 6 cut(s) 654, 846, 1157, 1368, 1452, 1563
AspLEI GCGC 2 cut(s) 446, 2132
AspS9I GGNCC 3 cut(s) 24, 227, 2956
AsuHPI GGTGA 3 cut(s) 443, 962, 2194
AsuII TTCGAA 1 cut(s) 1898
AvaI CYCGRG 2 cut(s) 904, 1510
AvaII GGWCC 3 cut(s) 24, 227, 2956
BanI GGYRCC 1 cut(s) 2398
BanII GRGCYC 2 cut(s) 1318, 1546
BarI GAAGNNNNNNTAC 2 cut(s) 1743, 1775
BauI CACGAG 1 cut(s) 1702
BbsI GAAGAC 2 cut(s) 1684, 2715
Bbv12I GWGCWC 2 cut(s) 1546, 1623
BbvCI CCTCAGC 1 cut(s) 2211
BccI CCATC 4 cut(s) 25, 556, 1082, 3188
BceAI ACGGC 1 cut(s) 751
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 2 cut(s) 796, 2820
BciVI GTATCC 2 cut(s) 588, 1648
BclI TGATCA 2 cut(s) 2229, 2322
BcoDI GTCTC 5 cut(s) 23, 333, 581, 1376, 1705
BcuI ACTAGT 1 cut(s) 2503
BfmI CTRYAG 4 cut(s) 439, 1612, 2060, 2121
BfoI RGCGCY 1 cut(s) 2133
BfrI CTTAAG 1 cut(s) 2632
BfuAI ACCTGC 3 cut(s) 1449, 1535, 2053
BfuI GTATCC 2 cut(s) 588, 1648
BglI GCCNNNNNGGC 1 cut(s) 2448
BglII AGATCT 1 cut(s) 3138
BlpI GCTNAGC 1 cut(s) 1638
BmcAI AGTACT 1 cut(s) 2427
Bme1390I CCNGG 2 cut(s) 796, 2820
Bme18I GGWCC 3 cut(s) 24, 227, 2956
BmeT110I CYCGRG 2 cut(s) 904, 1510
BmgT120I GGNCC 3 cut(s) 24, 227, 2956
BmiI GGNNCC 2 cut(s) 1188, 2400
BmrFI CCNGG 2 cut(s) 796, 2820
BmrI ACTGGG 1 cut(s) 2202
BmuI ACTGGG 1 cut(s) 2202
BpiI GAAGAC 2 cut(s) 1684, 2715
BpmI CTGGAG 1 cut(s) 2109
Bpu10I CCTNAGC 3 cut(s) 2211, 2511, 2517
Bpu1102I GCTNAGC 1 cut(s) 1638
Bpu14I TTCGAA 1 cut(s) 1898
BpuEI CTTGAG 3 cut(s) 337, 959, 1557
Bsa29I ATCGAT 2 cut(s) 717, 3175
BsaAI YACGTR 2 cut(s) 1003, 3077
BsaBI GATNNNNATC 3 cut(s) 207, 2020, 3171
BsaI GGTCTC 1 cut(s) 333
BsaJI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1548, 1578
Bsc4I CCNNNNNNNGG 3 cut(s) 193, 1038, 1312
Bse118I RCCGGY 1 cut(s) 2874
Bse1I ACTGG 8 cut(s) 535, 633, 1591, 1656, 2197, 2332, 3033, 3224
Bse3DI GCAATG 4 cut(s) 968, 1015, 1924, 3109
Bse8I GATNNNNATC 3 cut(s) 207, 2020, 3171
BseBI CCWGG 2 cut(s) 796, 2820
BseCI ATCGAT 2 cut(s) 717, 3175
BseDI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BseGI GGATG 4 cut(s) 202, 858, 1093, 2881
BseJI GATNNNNATC 3 cut(s) 207, 2020, 3171
BseLI CCNNNNNNNGG 3 cut(s) 193, 1038, 1312
BseMI GCAATG 4 cut(s) 968, 1015, 1924, 3109
BseNI ACTGG 8 cut(s) 535, 633, 1591, 1656, 2197, 2332, 3033, 3224
BseYI CCCAGC 1 cut(s) 946
BsgI GTGCAG 2 cut(s) 1247, 1513
Bsh1236I CGCG 1 cut(s) 2267
Bsh1285I CGRYCG 1 cut(s) 2875
BshFI GGCC 2 cut(s) 2823, 3045
BshNI GGYRCC 1 cut(s) 2398
BshVI ATCGAT 2 cut(s) 717, 3175
BsiEI CGRYCG 1 cut(s) 2875
BsiHKAI GWGCWC 2 cut(s) 1546, 1623
BsiHKCI CYCGRG 2 cut(s) 904, 1510
BsiSI CCGG 2 cut(s) 1284, 2875
BsiWI CGTACG 1 cut(s) 999
BslI CCNNNNNNNGG 3 cut(s) 193, 1038, 1312
BsmAI GTCTC 5 cut(s) 23, 333, 581, 1376, 1705
BsmI GAATGC 4 cut(s) 250, 270, 2320, 2858
BsnI GGCC 2 cut(s) 2823, 3045
Bso31I GGTCTC 1 cut(s) 333
BsoBI CYCGRG 2 cut(s) 904, 1510
Bsp119I TTCGAA 1 cut(s) 1898
Bsp1286I GDGCHC 3 cut(s) 1318, 1546, 1623
Bsp1407I TGTACA 1 cut(s) 3009
Bsp1720I GCTNAGC 1 cut(s) 1638
BspACI CCGC 4 cut(s) 79, 161, 2824, 2929
BspANI GGCC 2 cut(s) 2823, 3045
BspDI ATCGAT 2 cut(s) 717, 3175
BspFNI CGCG 1 cut(s) 2267
BspLI GGNNCC 2 cut(s) 1188, 2400
BspMAI CTGCAG 3 cut(s) 443, 2064, 2125
BspMI ACCTGC 3 cut(s) 1449, 1535, 2053
BspPI GGATC 4 cut(s) 2547, 2781, 2993, 3128
BspT104I TTCGAA 1 cut(s) 1898
BspT107I GGYRCC 1 cut(s) 2398
BspTI CTTAAG 1 cut(s) 2632
BspTNI GGTCTC 1 cut(s) 333
BsrBI CCGCTC 1 cut(s) 2929
BsrDI GCAATG 4 cut(s) 968, 1015, 1924, 3109
BsrFI RCCGGY 1 cut(s) 2874
BsrGI TGTACA 1 cut(s) 3009
BsrI ACTGG 8 cut(s) 535, 633, 1591, 1656, 2197, 2332, 3033, 3224
BssAI RCCGGY 1 cut(s) 2874
BssECI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BssNAI GTATAC 1 cut(s) 870
BssSI CACGAG 1 cut(s) 1702
BssT1I CCWWGG 2 cut(s) 1663, 2178
Bst1107I GTATAC 1 cut(s) 870
Bst2BI CACGAG 1 cut(s) 1702
Bst2UI CCWGG 2 cut(s) 796, 2820
Bst4CI ACNGT 5 cut(s) 1196, 2048, 2570, 2960, 3007
Bst6I CTCTTC 1 cut(s) 2264
BstAFI CTTAAG 1 cut(s) 2632
BstAPI GCANNNNNTGC 3 cut(s) 182, 1727, 3108
BstAUI TGTACA 1 cut(s) 3009
BstBAI YACGTR 2 cut(s) 1003, 3077
BstBI TTCGAA 1 cut(s) 1898
BstC8I GCNNGC 5 cut(s) 125, 439, 1619, 2469, 2931
BstF5I GGATG 4 cut(s) 202, 858, 1093, 2881
BstFNI CGCG 1 cut(s) 2267
BstH2I RGCGCY 1 cut(s) 2133
BstHHI GCGC 2 cut(s) 446, 2132
BstMAI GTCTC 5 cut(s) 23, 333, 581, 1376, 1705
BstMCI CGRYCG 1 cut(s) 2875
BstNI CCWGG 2 cut(s) 796, 2820
BstNSI RCATGY 3 cut(s) 618, 1478, 2743
BstSCI CCNGG 2 cut(s) 794, 2818
BstSFI CTRYAG 4 cut(s) 439, 1612, 2060, 2121
BstSNI TACGTA 1 cut(s) 1003
BstUI CGCG 1 cut(s) 2267
BstV2I GAAGAC 2 cut(s) 1684, 2715
BstX2I RGATCY 3 cut(s) 2539, 2998, 3138
BstYI RGATCY 3 cut(s) 2539, 2998, 3138
BstZ17I GTATAC 1 cut(s) 870
Bsu15I ATCGAT 2 cut(s) 717, 3175
BsuI GTATCC 2 cut(s) 588, 1648
BsuRI GGCC 2 cut(s) 2823, 3045
BsuTUI ATCGAT 2 cut(s) 717, 3175
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 4 cut(s) 202, 858, 1093, 2881
BtsI GCAGTG 2 cut(s) 2057, 2754
BtsIMutI CAGTG 6 cut(s) 1133, 1683, 2057, 2325, 2754, 3012
BveI ACCTGC 3 cut(s) 1449, 1535, 2053
Cac8I GCNNGC 5 cut(s) 125, 439, 1619, 2469, 2931
CaiI CAGNNNCTG 2 cut(s) 2210, 2753
CfoI GCGC 2 cut(s) 446, 2132
Cfr10I RCCGGY 1 cut(s) 2874
Cfr13I GGNCC 3 cut(s) 24, 227, 2956
ClaI ATCGAT 2 cut(s) 717, 3175
CseI GACGC 1 cut(s) 2273
Csp6I GTAC 7 cut(s) 1000, 1081, 2044, 2426, 3010, 3063, 3161
CviQI GTAC 7 cut(s) 1000, 1081, 2044, 2426, 3010, 3063, 3161
DraI TTTAAA 1 cut(s) 1465
EaeI YGGCCR 1 cut(s) 2821
Eam1104I CTCTTC 1 cut(s) 2264
EarI CTCTTC 1 cut(s) 2264
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 1544
Eco105I TACGTA 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 1663, 2178
Eco24I GRGCYC 2 cut(s) 1318, 1546
Eco31I GGTCTC 1 cut(s) 333
Eco32I GATATC 2 cut(s) 1113, 2022
Eco47I GGWCC 3 cut(s) 24, 227, 2956
Eco47III AGCGCT 1 cut(s) 2131
Eco53kI GAGCTC 1 cut(s) 1544
Eco57I CTGAAG 5 cut(s) 90, 858, 1707, 1770, 2727
Eco88I CYCGRG 2 cut(s) 904, 1510
EcoICRI GAGCTC 1 cut(s) 1544
EcoO109I RGGNCCY 1 cut(s) 24
EcoRI GAATTC 4 cut(s) 1788, 1839, 2845, 2906
EcoRII CCWGG 2 cut(s) 794, 2818
EcoRV GATATC 2 cut(s) 1113, 2022
EcoT14I CCWWGG 2 cut(s) 1663, 2178
EcoT22I ATGCAT 5 cut(s) 1476, 1606, 1886, 2082, 2860
EcoT38I GRGCYC 2 cut(s) 1318, 1546
ErhI CCWWGG 2 cut(s) 1663, 2178
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FauI CCCGC 1 cut(s) 2922
FauNDI CATATG 2 cut(s) 1270, 1606
FbaI TGATCA 2 cut(s) 2229, 2322
FblI GTMKAC 2 cut(s) 869, 1861
FokI GGATG 4 cut(s) 209, 845, 1100, 2868
FriOI GRGCYC 2 cut(s) 1318, 1546
GlaI GCGC 2 cut(s) 445, 2131
GsaI CCCAGC 1 cut(s) 950
GsuI CTGGAG 1 cut(s) 2109
HaeII RGCGCY 1 cut(s) 2133
HaeIII GGCC 2 cut(s) 2823, 3045
HapII CCGG 2 cut(s) 1284, 2875
HgaI GACGC 1 cut(s) 2273
HhaI GCGC 2 cut(s) 446, 2132
Hin6I GCGC 2 cut(s) 444, 2130
HinP1I GCGC 2 cut(s) 444, 2130
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HpaII CCGG 2 cut(s) 1284, 2875
HphI GGTGA 3 cut(s) 443, 962, 2194
Hpy166II GTNNAC 7 cut(s) 418, 528, 870, 1707, 1862, 2109, 3010
Hpy188III TCNNGA 6 cut(s) 357, 581, 862, 876, 904, 1162
Hpy8I GTNNAC 7 cut(s) 418, 528, 870, 1707, 1862, 2109, 3010
HpyAV CCTTC 4 cut(s) 215, 1304, 2456, 2788
HpyCH4III ACNGT 5 cut(s) 1196, 2048, 2570, 2960, 3007
HpyCH4IV ACGT 6 cut(s) 265, 998, 1002, 2724, 3076, 3131
HpySE526I ACGT 6 cut(s) 265, 998, 1002, 2724, 3076, 3131
HspAI GCGC 2 cut(s) 444, 2130
Ksp22I TGATCA 2 cut(s) 2229, 2322
LmnI GCTCC 2 cut(s) 1541, 3209
MaeII ACGT 6 cut(s) 265, 998, 1002, 2724, 3076, 3131
MaeIII GTNAC 7 cut(s) 34, 398, 1018, 1198, 1927, 2410, 2960
MbiI CCGCTC 1 cut(s) 2929
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 3 cut(s) 2539, 2998, 3138
MhlI GDGCHC 3 cut(s) 1318, 1546, 1623
MlyI GAGTC 3 cut(s) 825, 1674, 2705
MmeI TCCRAC 3 cut(s) 1519, 2810, 3026
Mph1103I ATGCAT 5 cut(s) 1476, 1606, 1886, 2082, 2860
MroXI GAANNNNTTC 6 cut(s) 654, 846, 1157, 1368, 1452, 1563
MslI CAYNNNNRTG 7 cut(s) 132, 216, 863, 1269, 1628, 2346, 2417
MspA1I CMGCKG 1 cut(s) 958
MspCI CTTAAG 1 cut(s) 2632
MspI CCGG 2 cut(s) 1284, 2875
MspR9I CCNGG 2 cut(s) 796, 2820
MunI CAATTG 1 cut(s) 1203
Mva1269I GAATGC 4 cut(s) 250, 270, 2320, 2858
MvaI CCWGG 2 cut(s) 796, 2820
MvnI CGCG 1 cut(s) 2267
NdeI CATATG 2 cut(s) 1270, 1606
NlaIV GGNNCC 2 cut(s) 1188, 2400
NmuCI GTSAC 2 cut(s) 34, 2410
NsiI ATGCAT 5 cut(s) 1476, 1606, 1886, 2082, 2860
NspI RCATGY 3 cut(s) 618, 1478, 2743
NspV TTCGAA 1 cut(s) 1898
OliI CACNNNNGTG 2 cut(s) 132, 2417
PaeR7I CTCGAG 1 cut(s) 904
PciI ACATGT 1 cut(s) 2739
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 4 cut(s) 250, 270, 2320, 2858
PdmI GAANNNNTTC 6 cut(s) 654, 846, 1157, 1368, 1452, 1563
Pfl23II CGTACG 1 cut(s) 999
PflMI CCANNNNNTGG 1 cut(s) 193
PleI GAGTC 3 cut(s) 825, 1674, 2705
PpsI GAGTC 3 cut(s) 825, 1674, 2705
Ppu21I YACGTR 2 cut(s) 1003, 3077
PpuMI RGGWCCY 1 cut(s) 24
PscI ACATGT 1 cut(s) 2739
PshBI ATTAAT 1 cut(s) 2789
Psp124BI GAGCTC 1 cut(s) 1546
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 794, 2818
PspFI CCCAGC 1 cut(s) 946
PspGI CCWGG 2 cut(s) 794, 2818
PspLI CGTACG 1 cut(s) 999
PspN4I GGNNCC 2 cut(s) 1188, 2400
PspPI GGNCC 3 cut(s) 24, 227, 2956
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 3 cut(s) 443, 2064, 2125
PstNI CAGNNNCTG 2 cut(s) 2210, 2753
PsuI RGATCY 3 cut(s) 2539, 2998, 3138
PvuII CAGCTG 1 cut(s) 958
RsaI GTAC 7 cut(s) 1001, 1082, 2045, 2427, 3011, 3064, 3162
RsaNI GTAC 7 cut(s) 1000, 1081, 2044, 2426, 3010, 3063, 3161
RseI CAYNNNNRTG 7 cut(s) 132, 216, 863, 1269, 1628, 2346, 2417
SacI GAGCTC 1 cut(s) 1546
Sau96I GGNCC 3 cut(s) 24, 227, 2956
ScaI AGTACT 1 cut(s) 2427
SchI GAGTC 3 cut(s) 825, 1674, 2705
ScrFI CCNGG 2 cut(s) 796, 2820
SduI GDGCHC 3 cut(s) 1318, 1546, 1623
SfcI CTRYAG 4 cut(s) 439, 1612, 2060, 2121
Sfr274I CTCGAG 1 cut(s) 904
SfuI TTCGAA 1 cut(s) 1898
SinI GGWCC 3 cut(s) 24, 227, 2956
SlaI CTCGAG 1 cut(s) 904
SmiMI CAYNNNNRTG 7 cut(s) 132, 216, 863, 1269, 1628, 2346, 2417
SmlI CTYRAG 5 cut(s) 316, 904, 974, 1572, 2632
SmoI CTYRAG 5 cut(s) 316, 904, 974, 1572, 2632
SnaBI TACGTA 1 cut(s) 1003
SpeI ACTAGT 1 cut(s) 2503
SsiI CCGC 4 cut(s) 79, 161, 2824, 2929
SstI GAGCTC 1 cut(s) 1546
StyD4I CCNGG 2 cut(s) 794, 2818
StyI CCWWGG 2 cut(s) 1663, 2178
TaaI ACNGT 5 cut(s) 1196, 2048, 2570, 2960, 3007
TaiI ACGT 6 cut(s) 268, 1001, 1005, 2727, 3079, 3134
TaqI TCGA 9 cut(s) 717, 834, 905, 1308, 1792, 1898, 2083, 2243, 3175
TatI WGTACW 2 cut(s) 2425, 3009
TauI GCSGC 1 cut(s) 2826
TscAI CASTG 6 cut(s) 1133, 1690, 2064, 2332, 2754, 3012
TseFI GTSAC 2 cut(s) 34, 2410
Tsp45I GTSAC 2 cut(s) 34, 2410
TspGWI ACGGA 1 cut(s) 662
TspRI CASTG 6 cut(s) 1133, 1690, 2064, 2332, 2754, 3012
Van91I CCANNNNNTGG 1 cut(s) 193
Vha464I CTTAAG 1 cut(s) 2632
VpaK11BI GGWCC 3 cut(s) 24, 227, 2956
VspI ATTAAT 1 cut(s) 2789
XapI RAATTY 7 cut(s) 879, 1364, 1788, 1839, 2579, 2845, 2906
XbaI TCTAGA 1 cut(s) 356
XceI RCATGY 3 cut(s) 618, 1478, 2743
XhoI CTCGAG 1 cut(s) 904
XmiI GTMKAC 2 cut(s) 869, 1861
XmnI GAANNNNTTC 6 cut(s) 654, 846, 1157, 1368, 1452, 1563
ZrmI AGTACT 1 cut(s) 2427
Zsp2I ATGCAT 5 cut(s) 1476, 1606, 1886, 2082, 2860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.