Rroxscaffold_1G00034510

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0016595
Physical Location & Seq
Reverse (-)
50112798 .. 50119502
6705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00034510.1

Sequence Viewer

Length: 3237 bp
ATGAGTGGGAGTGGGTGTCTCAGGTCCTTGATGCTCACTGTAAACCAATGCCACAACCCTATGTTCTTCCATCGCTACCGGCGTCATTTCACAACCAAACCCAAATCCAAACCCACCTCAGTTATCAATCTTCGCAAGCACTTTCGTGGGAGTGTAGTTAGTTTGAGCTTGGCGGAATATAGTAATGCAGCCAATGCTCGTGTTTCGGATGATTATCTAGTTCATCAAAATGAAGGTGGTCCAAAGGGGATCGATTTGTTGCATTCTATGGAAGCACGTTGCATTCGTGCCAATTCTCAGACATATATTTGGCTTTTGAAAGGGTGCTTGAGTTCTGGGTCTTTGTTAGATGCTAGGAAACTTCATTCTAGGATTTTGAAGTTGGGTTTTGGTGGAGAATATGAAATAAGTGACCTATTTGTCGGTGTTTATCTTGCGAATGGTGATTCATGCAGTGCAGTAAAGGTTTTCGAGGATCTACCATATAGAAGTTTATTCTCATGGAATAACATAATTCATGGGTTTCTTGCAAAGAAGTTGACGGGTCAAGTATTGGGTTTCTTTTCCCGAATGGTAGCAGAAAATGTGCGTCCTGATGAGACCATGTTTGCAGGAGTTTTAAGGGCGTGTGGTGGTCTTAACGCCAGTATCCATTATGTGGAACAGATTCATGCTAGGATTATCCGCCGTTGTTTTGGTACTAGTTTACTTGTATGTAATCCCTTGATCGATTTGTATGCTAAGAACGGCTCTGTGGATTCTGCTAAAAAAGTCTTTGATAAGTTGAATTTTAGGGATAGTGTTTCCTGGGTGGCTATAATTTCTGGCTTATCTCGTAATGGACGAGAAGAAGAAGCTGTTCTCCTATTCATCCAGATGCATACTTCAGGAATTTTCCCAACTCCTTATGTTTTCTCAAGCGTGATTAGTGCCTGTGCAAAAATAGAGTTATTTGAGCTGGGTGAGCAGCTGCAATGCCTTGTTTTCAAGGGAGGCTTTTCTTGTGAAACATACGTATGCAATGCTCTTGTAACATTATATTCCCGTTCAGGGAACTTCATATCTGCTGAACAAGTTTTCAATACAATGTGGCACAGGGATGGAGTGTCATATAACTCATTGATATCTGGGCTAGCTCAGTGTGGATTTAGTGATAGAGCTCTGGAATTGTTCAAGAAAATGCAGAGTGACTGTATGGAACCTGACTGTGTAACAATTGCTAGTCTTTTGAGTGCCTGTGCATCAGTTGGTTATCTGTATAAGGGGAAACAACTCCACTCATATGCAATTAAAGCAGGAATGTCATCAGATATAATCCTCGAAGGGTCTCTGCTTGATCTTTATGTGAAATGCTCAGATTTGCAAACCGCCTATGAATTTTTCCTTACAACAGAGACAGAGAATGTTGTCCTGTGGAATGTGATGCTAGTTGCTTATGGGCAGCAAGATGACCTGATCGAATCGTTTCATATTTTTAAACAAATGCATGCTGAGGGTATGATTCCTAATCAATATACCTATCCGAGTATTTTGAGAACCTGTACTTCTGTTGGAGCTCTCAATTTAGGAGAACAGGTTCATACTCAAGCCATAAAAACTGGCTTTCAGTTTAATGCATATGTCTGTAGCGTGCTCATTGATATGTATGCTAAGCATGGAAAACTGGATACTGCCCTTGGAATACTCAGAAGACTCACTGAAGATGATGTTGTTTCGTGGACAGCTATGATTGCAGGGTACGCACAGCATGATTTGTTTGCTGAAGCTCTTTTACTTTTTGAAGAAATGCAAAACCGAGGGATTCGATCTGACAACATAGGATTTTCAAGTGCAATTAGTTCATGTGCTGGTATTCAAGCACTCAATCAAGGTAGACAGATTCATGCTCAGTCATGCATATCTGGTTATTCGACTGATCTTTCCGTTGGCAATGCACTTGTTACCCTTTATGCTAGATGTGGTAGAATATGGGAAGCCTACCAAGCATTTGAGAAAATTGATGTGAAAGATAATATATCATGGAATGGACTGATATCAGGGTTTGGACAGTGTGGGTACTGTGAGGAAGCACTGCAGGTATTTTCTCAGATGCATCGAGCTGGAGTAGAAGCTAATTTGTTCACATTTGGCTCTGCTGTCAGCGCTGCTGCTAATTTAGCAAATATAAAACAAGGAGAGCAGATTCATGCCATGGTGATAAAAACTGGGAACAATTCAGAAGCTGAGGTTTCCAATGCTTTAATCACATTATACTCAAAGTGTGGTAGTGTTGATGATGCGAAGAGAGAGTTTATTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAGCATGGGCATGGTATTGAAGCACTACATCTGTTTGAGCAGATGAAACAACTTGGTGTGGTGCCAAGCCATGTCACATTTGTGGGAGTACTTTCAGCTTGTAGCCATGTAGGCTTGGTAAGTGAGGGGCTTGCATACTTTGAATCCATGAGCAAAGAGCATGGACTAGTGCCTAAGCCTGAGCATTATGCTTGTGTTGTTGATCTTCTTAGTCGAGCTGGTTCTTTGAGCCAGGCAAGAAAATTCATAACTGAGATGCCAATAAAACCAGATTCAATGATTTGGAGGACTCTCTTAAGTGCTTGTATAGCTAAAAAGAACACAGAAATTGGGGAGGTTGCTGCCCGTCATCTTCTAGAACTGGAACCAGAAGACTCAGCAACTTACGTTCTCATATCAAACATGTATGCAGTGGCAGGAATGTGGGGTTATAGGGATCAGGCAAGGCAATTAATGAAGGAAAGGGGTGTGAAGAAAGAGCCTGGCCGCAGTTGGATTGAGGTAAAGAATTTGGTTCATGCATTCTATGTTGGTGACCGGCTGCACCCACTTGCCAATAAGATATATGAATTCTTAGGGGATTTGAATGAGCGGGCAGCTGAAATTGGTTATGTTGAGGACCGTAACAACCTCTGGAATGACATGGAACAACAGCATAAAGATCCAACAGTGTACATTCATAGCGAGAAATTGGCAATAGCTTTTGGCCTGATTAGCCTATCTGATACGATTCCCATACGTGTAATTAAGAATCTCCGTGTTTGTAATGATTGCCATAATTGGATCAAGCATGCGTCTAAGATCTCAAAAAGAACTATCATAGTACGAGATGCATATCGTTTTCATCATTTTAAAGATGGTGTTTGCTCTTGTAAAGATTACTGGTAA

Protein Analysis

1078

Amino Acids

120.23

Weight (kDa)

6.76

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 266 - 314 7.4e-08 PPR repeat family
PPR_2 PF13041 367 - 414 1.5e-08 PPR repeat family
PPR_1 PF12854 367 - 395 6.5e-06 PPR repeat
PPR PF01535 369 - 397 5.6e-08 PPR repeat
PPR_2 PF13041 468 - 514 1.1e-07 PPR repeat family
PPR_2 PF13041 569 - 616 2e-09 PPR repeat family
PPR PF01535 571 - 601 9.8e-08 PPR repeat
PPR_2 PF13041 670 - 714 9.1e-08 PPR repeat family
PPR PF01535 672 - 702 2.1e-07 PPR repeat
PPR_2 PF13041 771 - 818 4.6e-11 PPR repeat family
PPR PF01535 773 - 803 2.9e-08 PPR repeat
E_motif PF20431 890 - 950 4.5e-19 E motif
DYW_deaminase PF14432 986 - 1078 2e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13650
fragaria_vesca FvH4_3g25260
malus_domestica MD12G1002600.v1.1
prunus_persica Prupe.7G002600_v2.0.a1
pyrus_communis pycom12g00230
rosa_chinensis RchiOBHm_Chr5g0046881
rosa_laevigata RLG00000034452
rosa_roxburghii Rroxscaffold_1G00034510
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana Rw5G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 419
Acc36I ACCTGC 1 cut(s) 2065
AccB1I GGYRCC 1 cut(s) 2410
AccB7I CCANNNNNTGG 1 cut(s) 658
AccBSI CCGCTC 1 cut(s) 2941
AccI GTMKAC 1 cut(s) 1873
AciI CCGC 5 cut(s) 173, 685, 1368, 2836, 2941
AclWI GGATC 5 cut(s) 257, 483, 2793, 3005, 3140
AcoI YGGCCR 1 cut(s) 2833
AcsI RAATTY 6 cut(s) 787, 891, 1376, 2591, 2857, 2918
AcuI CTGAAG 3 cut(s) 870, 1719, 1782
AcyI GRCGYC 1 cut(s) 82
AfaI GTAC 7 cut(s) 700, 1543, 1739, 2057, 2439, 3023, 3174
AfeI AGCGCT 1 cut(s) 2143
AfiI CCNNNNNNNGG 2 cut(s) 658, 1050
AflII CTTAAG 1 cut(s) 2644
AflIII ACRYGT 2 cut(s) 2751, 3088
AhlI ACTAGT 2 cut(s) 701, 2515
AjnI CCWGG 3 cut(s) 806, 2580, 2830
AleI CACNNNNGTG 2 cut(s) 144, 2429
AloI GAACNNNNNNTCC 2 cut(s) 1560, 1592
Alw21I GWGCWC 3 cut(s) 1162, 1558, 1635
Alw26I GTCTC 4 cut(s) 23, 593, 1332, 1388
AlwI GGATC 5 cut(s) 257, 483, 2793, 3005, 3140
AlwNI CAGNNNCTG 1 cut(s) 2222
Aor51HI AGCGCT 1 cut(s) 2143
AoxI GGCC 2 cut(s) 2833, 3055
ApeKI GCWGC 9 cut(s) 188, 967, 970, 1441, 2144, 2147, 2690, 2890, 2945
ApoI RAATTY 6 cut(s) 787, 891, 1376, 2591, 2857, 2918
AseI ATTAAT 1 cut(s) 2801
Asp700I GAANNNNTTC 4 cut(s) 666, 858, 1464, 1575
AspLEI GCGC 1 cut(s) 2144
AspS9I GGNCC 3 cut(s) 24, 239, 2968
AsuHPI GGTGA 4 cut(s) 455, 974, 2206, 2894
AsuNHI GCTAGC 1 cut(s) 1132
AvaII GGWCC 3 cut(s) 24, 239, 2968
BaeI ACNNNNGTAYC 2 cut(s) 631, 664
BanI GGYRCC 1 cut(s) 2410
BanII GRGCYC 2 cut(s) 1162, 1558
BarI GAAGNNNNNNTAC 2 cut(s) 1755, 1787
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 1696, 2727
Bbv12I GWGCWC 3 cut(s) 1162, 1558, 1635
BbvCI CCTCAGC 2 cut(s) 1491, 2223
BbvI GCAGC 9 cut(s) 200, 957, 979, 1453, 2131, 2134, 2677, 2877, 2957
BccI CCATC 3 cut(s) 78, 1094, 3200
BceAI ACGGC 2 cut(s) 672, 763
BcgI CGANNNNNNTGC 2 cut(s) 719, 753
BciT130I CCWGG 3 cut(s) 808, 2582, 2832
BciVI GTATCC 2 cut(s) 659, 1660
BcoDI GTCTC 4 cut(s) 23, 593, 1332, 1388
BcuI ACTAGT 2 cut(s) 701, 2515
BfmI CTRYAG 2 cut(s) 1624, 2072
BfoI RGCGCY 1 cut(s) 2145
BfrI CTTAAG 1 cut(s) 2644
BfuAI ACCTGC 1 cut(s) 2065
BfuI GTATCC 2 cut(s) 659, 1660
BglI GCCNNNNNGGC 1 cut(s) 2460
BglII AGATCT 1 cut(s) 3150
BlpI GCTNAGC 1 cut(s) 1650
BmcAI AGTACT 1 cut(s) 2439
Bme1390I CCNGG 3 cut(s) 808, 2582, 2832
Bme18I GGWCC 3 cut(s) 24, 239, 2968
BmgT120I GGNCC 3 cut(s) 24, 239, 2968
BmiI GGNNCC 3 cut(s) 1200, 2412, 2715
BmrFI CCNGG 3 cut(s) 808, 2582, 2832
BmrI ACTGGG 1 cut(s) 2214
BmtI GCTAGC 1 cut(s) 1136
BmuI ACTGGG 1 cut(s) 2214
BpiI GAAGAC 2 cut(s) 1696, 2727
BpmI CTGGAG 1 cut(s) 2121
Bpu10I CCTNAGC 4 cut(s) 1491, 2223, 2523, 2529
Bpu1102I GCTNAGC 1 cut(s) 1650
BpuEI CTTGAG 3 cut(s) 349, 901, 1569
Bsa29I ATCGAT 2 cut(s) 252, 729
BsaAI YACGTR 2 cut(s) 1015, 3089
BsaBI GATNNNNATC 2 cut(s) 213, 3183
BsaHI GRCGYC 1 cut(s) 82
BsaI GGTCTC 2 cut(s) 593, 1332
BsaJI CCNNGG 4 cut(s) 807, 1675, 1795, 2190
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1560, 1590
Bsc4I CCNNNNNNNGG 2 cut(s) 658, 1050
Bse118I RCCGGY 2 cut(s) 78, 2886
Bse1I ACTGG 7 cut(s) 645, 1603, 1668, 2209, 2344, 2715, 3236
Bse3DI GCAATG 3 cut(s) 980, 1027, 1936
Bse8I GATNNNNATC 2 cut(s) 213, 3183
BseBI CCWGG 3 cut(s) 808, 2582, 2832
BseCI ATCGAT 2 cut(s) 252, 729
BseDI CCNNGG 4 cut(s) 807, 1675, 1795, 2190
BseGI GGATG 3 cut(s) 214, 870, 1105
BseJI GATNNNNATC 2 cut(s) 213, 3183
BseLI CCNNNNNNNGG 2 cut(s) 658, 1050
BseMI GCAATG 3 cut(s) 980, 1027, 1936
BseNI ACTGG 7 cut(s) 645, 1603, 1668, 2209, 2344, 2715, 3236
BseXI GCAGC 9 cut(s) 200, 957, 979, 1453, 2131, 2134, 2677, 2877, 2957
BseYI CCCAGC 1 cut(s) 958
BsgI GTGCAG 2 cut(s) 477, 2876
BshFI GGCC 2 cut(s) 2835, 3057
BshNI GGYRCC 1 cut(s) 2410
BshVI ATCGAT 2 cut(s) 252, 729
BsiHKAI GWGCWC 3 cut(s) 1162, 1558, 1635
BsiSI CCGG 2 cut(s) 79, 2887
BslI CCNNNNNNNGG 2 cut(s) 658, 1050
BsmAI GTCTC 4 cut(s) 23, 593, 1332, 1388
BsmI GAATGC 4 cut(s) 262, 282, 2332, 2870
BsnI GGCC 2 cut(s) 2835, 3057
Bso31I GGTCTC 2 cut(s) 593, 1332
Bsp1286I GDGCHC 3 cut(s) 1162, 1558, 1635
Bsp1407I TGTACA 1 cut(s) 3021
Bsp1720I GCTNAGC 1 cut(s) 1650
Bsp19I CCATGG 1 cut(s) 2190
BspACI CCGC 5 cut(s) 173, 685, 1368, 2836, 2941
BspANI GGCC 2 cut(s) 2835, 3057
BspDI ATCGAT 2 cut(s) 252, 729
BspLI GGNNCC 3 cut(s) 1200, 2412, 2715
BspMAI CTGCAG 1 cut(s) 2076
BspMI ACCTGC 1 cut(s) 2065
BspOI GCTAGC 1 cut(s) 1136
BspPI GGATC 5 cut(s) 257, 483, 2793, 3005, 3140
BspT107I GGYRCC 1 cut(s) 2410
BspTI CTTAAG 1 cut(s) 2644
BspTNI GGTCTC 2 cut(s) 593, 1332
BsrBI CCGCTC 1 cut(s) 2941
BsrDI GCAATG 3 cut(s) 980, 1027, 1936
BsrFI RCCGGY 2 cut(s) 78, 2886
BsrGI TGTACA 1 cut(s) 3021
BsrI ACTGG 7 cut(s) 645, 1603, 1668, 2209, 2344, 2715, 3236
BssAI RCCGGY 2 cut(s) 78, 2886
BssECI CCNNGG 4 cut(s) 807, 1675, 1795, 2190
BssNI GRCGYC 1 cut(s) 82
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 1675, 2190
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 3 cut(s) 808, 2582, 2832
Bst4CI ACNGT 7 cut(s) 40, 1193, 1208, 2049, 2060, 2972, 3019
Bst6I CTCTTC 1 cut(s) 2276
BstACI GRCGYC 1 cut(s) 82
BstAFI CTTAAG 1 cut(s) 2644
BstAPI GCANNNNNTGC 1 cut(s) 194
BstAUI TGTACA 1 cut(s) 3021
BstBAI YACGTR 2 cut(s) 1015, 3089
BstC8I GCNNGC 7 cut(s) 137, 1134, 1488, 1631, 2481, 2943, 3141
BstDSI CCRYGG 1 cut(s) 2190
BstEII GGTNACC 1 cut(s) 2882
BstF5I GGATG 3 cut(s) 214, 870, 1105
BstH2I RGCGCY 1 cut(s) 2145
BstHHI GCGC 1 cut(s) 2144
BstMAI GTCTC 4 cut(s) 23, 593, 1332, 1388
BstNI CCWGG 3 cut(s) 808, 2582, 2832
BstNSI RCATGY 3 cut(s) 1490, 2755, 3143
BstPI GGTNACC 1 cut(s) 2882
BstSCI CCNGG 3 cut(s) 806, 2580, 2830
BstSFI CTRYAG 2 cut(s) 1624, 2072
BstSNI TACGTA 1 cut(s) 1015
BstV1I GCAGC 9 cut(s) 200, 957, 979, 1453, 2131, 2134, 2677, 2877, 2957
BstV2I GAAGAC 2 cut(s) 1696, 2727
BstX2I RGATCY 3 cut(s) 475, 3010, 3150
BstYI RGATCY 3 cut(s) 475, 3010, 3150
Bsu15I ATCGAT 2 cut(s) 252, 729
BsuI GTATCC 2 cut(s) 659, 1660
BsuRI GGCC 2 cut(s) 2835, 3057
BsuTUI ATCGAT 2 cut(s) 252, 729
BtgI CCRYGG 1 cut(s) 2190
BtgZI GCGATG 1 cut(s) 56
BtsCI GGATG 3 cut(s) 214, 870, 1105
BtsI GCAGTG 3 cut(s) 460, 2069, 2766
BtsIMutI CAGTG 9 cut(s) 36, 460, 1145, 1695, 2054, 2069, 2337, 2766, 3024
BveI ACCTGC 1 cut(s) 2065
Cac8I GCNNGC 7 cut(s) 137, 1134, 1488, 1631, 2481, 2943, 3141
CaiI CAGNNNCTG 1 cut(s) 2222
CfoI GCGC 1 cut(s) 2144
Cfr10I RCCGGY 2 cut(s) 78, 2886
Cfr13I GGNCC 3 cut(s) 24, 239, 2968
ClaI ATCGAT 2 cut(s) 252, 729
CseI GACGC 3 cut(s) 71, 578, 3132
Csp6I GTAC 7 cut(s) 699, 1542, 1738, 2056, 2438, 3022, 3173
CviQI GTAC 7 cut(s) 699, 1542, 1738, 2056, 2438, 3022, 3173
DraI TTTAAA 2 cut(s) 1477, 3202
DrdI GACNNNNNNGTC 1 cut(s) 419
DseDI GACNNNNNNGTC 1 cut(s) 419
EaeI YGGCCR 1 cut(s) 2833
Eam1104I CTCTTC 1 cut(s) 2276
EarI CTCTTC 1 cut(s) 2276
EciI GGCGGA 2 cut(s) 188, 674
Ecl136II GAGCTC 2 cut(s) 1160, 1556
Eco105I TACGTA 1 cut(s) 1015
Eco130I CCWWGG 2 cut(s) 1675, 2190
Eco24I GRGCYC 2 cut(s) 1162, 1558
Eco31I GGTCTC 2 cut(s) 593, 1332
Eco32I GATATC 2 cut(s) 1125, 2034
Eco47I GGWCC 3 cut(s) 24, 239, 2968
Eco47III AGCGCT 1 cut(s) 2143
Eco53kI GAGCTC 2 cut(s) 1160, 1556
Eco57I CTGAAG 3 cut(s) 870, 1719, 1782
Eco91I GGTNACC 1 cut(s) 2882
EcoICRI GAGCTC 2 cut(s) 1160, 1556
EcoO109I RGGNCCY 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 2882
EcoRI GAATTC 1 cut(s) 2918
EcoRII CCWGG 3 cut(s) 806, 2580, 2830
EcoRV GATATC 2 cut(s) 1125, 2034
EcoT14I CCWWGG 2 cut(s) 1675, 2190
EcoT22I ATGCAT 7 cut(s) 882, 1488, 1618, 1898, 2094, 2872, 3184
EcoT38I GRGCYC 2 cut(s) 1162, 1558
ErhI CCWWGG 2 cut(s) 1675, 2190
FalI AAGNNNNNCTT 2 cut(s) 980, 1012
FauI CCCGC 1 cut(s) 2934
FauNDI CATATG 2 cut(s) 1282, 1618
FbaI TGATCA 1 cut(s) 2334
FblI GTMKAC 1 cut(s) 1873
FokI GGATG 3 cut(s) 221, 857, 1112
FriOI GRGCYC 2 cut(s) 1162, 1558
GlaI GCGC 1 cut(s) 2143
GsaI CCCAGC 1 cut(s) 962
GsuI CTGGAG 1 cut(s) 2121
HaeII RGCGCY 1 cut(s) 2145
HaeIII GGCC 2 cut(s) 2835, 3057
HapII CCGG 2 cut(s) 79, 2887
HgaI GACGC 3 cut(s) 71, 578, 3132
HhaI GCGC 1 cut(s) 2144
Hin1I GRCGYC 1 cut(s) 82
Hin6I GCGC 1 cut(s) 2142
HinP1I GCGC 1 cut(s) 2142
HincII GTYRAC 1 cut(s) 540
HindII GTYRAC 1 cut(s) 540
HpaII CCGG 2 cut(s) 79, 2887
HphI GGTGA 4 cut(s) 455, 974, 2206, 2894
Hpy166II GTNNAC 7 cut(s) 43, 540, 707, 1719, 1874, 2121, 3022
Hpy188III TCNNGA 8 cut(s) 567, 593, 874, 888, 1163, 1174, 2705, 2983
Hpy8I GTNNAC 7 cut(s) 43, 540, 707, 1719, 1874, 2121, 3022
HpyAV CCTTC 3 cut(s) 227, 1316, 2800
HpyCH4III ACNGT 7 cut(s) 40, 1193, 1208, 2049, 2060, 2972, 3019
HpyCH4IV ACGT 4 cut(s) 277, 1014, 2736, 3088
HpySE526I ACGT 4 cut(s) 277, 1014, 2736, 3088
Hsp92I GRCGYC 1 cut(s) 82
HspAI GCGC 1 cut(s) 2142
Ksp22I TGATCA 1 cut(s) 2334
LmnI GCTCC 1 cut(s) 1553
Lsp1109I GCAGC 9 cut(s) 200, 957, 979, 1453, 2131, 2134, 2677, 2877, 2957
MaeII ACGT 4 cut(s) 277, 1014, 2736, 3088
MaeIII GTNAC 8 cut(s) 410, 1030, 1187, 1210, 1939, 2422, 2882, 2972
MbiI CCGCTC 1 cut(s) 2941
MfeI CAATTG 1 cut(s) 1215
MflI RGATCY 3 cut(s) 475, 3010, 3150
MhlI GDGCHC 3 cut(s) 1162, 1558, 1635
MlyI GAGTC 3 cut(s) 1686, 2632, 2717
MmeI TCCRAC 3 cut(s) 1531, 2822, 3038
Mph1103I ATGCAT 7 cut(s) 882, 1488, 1618, 1898, 2094, 2872, 3184
MroXI GAANNNNTTC 4 cut(s) 666, 858, 1464, 1575
MslI CAYNNNNRTG 9 cut(s) 144, 228, 875, 1015, 1098, 1281, 1640, 2358, 2429
MspA1I CMGCKG 2 cut(s) 970, 2948
MspCI CTTAAG 1 cut(s) 2644
MspI CCGG 2 cut(s) 79, 2887
MspR9I CCNGG 3 cut(s) 808, 2582, 2832
MunI CAATTG 1 cut(s) 1215
Mva1269I GAATGC 4 cut(s) 262, 282, 2332, 2870
MvaI CCWGG 3 cut(s) 808, 2582, 2832
NcoI CCATGG 1 cut(s) 2190
NdeI CATATG 2 cut(s) 1282, 1618
NheI GCTAGC 1 cut(s) 1132
NlaIV GGNNCC 3 cut(s) 1200, 2412, 2715
NmuCI GTSAC 4 cut(s) 410, 1187, 2422, 2882
NsiI ATGCAT 7 cut(s) 882, 1488, 1618, 1898, 2094, 2872, 3184
NspI RCATGY 3 cut(s) 1490, 2755, 3143
OliI CACNNNNGTG 2 cut(s) 144, 2429
PaeI GCATGC 2 cut(s) 1490, 3143
PciI ACATGT 1 cut(s) 2751
PcsI WCGNNNNNNNCGW 3 cut(s) 79, 283, 841
PctI GAATGC 4 cut(s) 262, 282, 2332, 2870
PdmI GAANNNNTTC 4 cut(s) 666, 858, 1464, 1575
PflMI CCANNNNNTGG 1 cut(s) 658
PleI GAGTC 3 cut(s) 1686, 2632, 2717
PpsI GAGTC 3 cut(s) 1686, 2632, 2717
Ppu21I YACGTR 2 cut(s) 1015, 3089
PpuMI RGGWCCY 1 cut(s) 24
PscI ACATGT 1 cut(s) 2751
PshBI ATTAAT 1 cut(s) 2801
Psp124BI GAGCTC 2 cut(s) 1162, 1558
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 3 cut(s) 806, 2580, 2830
PspEI GGTNACC 1 cut(s) 2882
PspFI CCCAGC 1 cut(s) 958
PspGI CCWGG 3 cut(s) 806, 2580, 2830
PspN4I GGNNCC 3 cut(s) 1200, 2412, 2715
PspPI GGNCC 3 cut(s) 24, 239, 2968
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 1 cut(s) 2076
PstNI CAGNNNCTG 1 cut(s) 2222
PsuI RGATCY 3 cut(s) 475, 3010, 3150
PvuII CAGCTG 2 cut(s) 970, 2948
RsaI GTAC 7 cut(s) 700, 1543, 1739, 2057, 2439, 3023, 3174
RsaNI GTAC 7 cut(s) 699, 1542, 1738, 2056, 2438, 3022, 3173
RseI CAYNNNNRTG 9 cut(s) 144, 228, 875, 1015, 1098, 1281, 1640, 2358, 2429
SacI GAGCTC 2 cut(s) 1162, 1558
Sau96I GGNCC 3 cut(s) 24, 239, 2968
ScaI AGTACT 1 cut(s) 2439
SchI GAGTC 3 cut(s) 1686, 2632, 2717
ScrFI CCNGG 3 cut(s) 808, 2582, 2832
SduI GDGCHC 3 cut(s) 1162, 1558, 1635
SfcI CTRYAG 2 cut(s) 1624, 2072
SinI GGWCC 3 cut(s) 24, 239, 2968
SmiMI CAYNNNNRTG 9 cut(s) 144, 228, 875, 1015, 1098, 1281, 1640, 2358, 2429
SmlI CTYRAG 4 cut(s) 328, 916, 1584, 2644
SmoI CTYRAG 4 cut(s) 328, 916, 1584, 2644
SnaBI TACGTA 1 cut(s) 1015
SpeI ACTAGT 2 cut(s) 701, 2515
SphI GCATGC 2 cut(s) 1490, 3143
SsiI CCGC 5 cut(s) 173, 685, 1368, 2836, 2941
SstI GAGCTC 2 cut(s) 1162, 1558
StyD4I CCNGG 3 cut(s) 806, 2580, 2830
StyI CCWWGG 2 cut(s) 1675, 2190
TaaI ACNGT 7 cut(s) 40, 1193, 1208, 2049, 2060, 2972, 3019
TaiI ACGT 4 cut(s) 280, 1017, 2739, 3091
TaqI TCGA 9 cut(s) 252, 471, 729, 1320, 1458, 1804, 1910, 2095, 2563
TatI WGTACW 3 cut(s) 1541, 2437, 3021
TauI GCSGC 1 cut(s) 2838
TscAI CASTG 9 cut(s) 43, 460, 1145, 1702, 2054, 2076, 2344, 2766, 3024
TseFI GTSAC 4 cut(s) 410, 1187, 2422, 2882
TseI GCWGC 9 cut(s) 188, 967, 970, 1441, 2144, 2147, 2690, 2890, 2945
Tsp45I GTSAC 4 cut(s) 410, 1187, 2422, 2882
TspGWI ACGGA 2 cut(s) 1912, 3095
TspRI CASTG 9 cut(s) 43, 460, 1145, 1702, 2054, 2076, 2344, 2766, 3024
Van91I CCANNNNNTGG 1 cut(s) 658
Vha464I CTTAAG 1 cut(s) 2644
VpaK11BI GGWCC 3 cut(s) 24, 239, 2968
VspI ATTAAT 1 cut(s) 2801
XapI RAATTY 6 cut(s) 787, 891, 1376, 2591, 2857, 2918
XbaI TCTAGA 1 cut(s) 2704
XceI RCATGY 3 cut(s) 1490, 2755, 3143
XmiI GTMKAC 1 cut(s) 1873
XmnI GAANNNNTTC 4 cut(s) 666, 858, 1464, 1575
ZrmI AGTACT 1 cut(s) 2439
Zsp2I ATGCAT 7 cut(s) 882, 1488, 1618, 1898, 2094, 2872, 3184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.