Rw5G029140

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0016595
Physical Location & Seq
Forward (+)
44410316 .. 44413611
3296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G029140.1

Sequence Viewer

Length: 3183 bp
ATGAGTGGGAGTGGGTGTCTCAGGTCCTTGATGCTCACTGTAAACCAATGCCACAACCCTATGTTCTTCCATCCATACCGGCTTCATTTCAGAACCAAACCCACCTCAGTTATCAATCTTCGCAAGCACTTTCGTGGGAGTGTAGTCAGTTTGAGCTTGGCGGAATATAGTAATGCAGCCAATGCTCGTGTTTCGGATGATTATCTAGTTCATCAAAATGAAGGTGGTCCAAAGGGGATCGATTTGTTGCATTCTATGGAAGCACGTTGCATTCGTGCTAATTCTCAGACATATATTTGGCTTTTAAAAGGGTGCTTGAGTTCTGGGTCTTTGTTAGATGCTAGAAAACTTCATTCTAGGATTTTGAAATTGGGTTTTGGTGGAGAATATGAAATAAGTGACCTATTTGTCGGTGTTTATCTTGCGAATGGTGATTCATGCAGTGCAGTAAAGGTTTTCGATGATCTACCATATAGAAGTTTATTCTCATGGAATAACATAATTCATGGGTTTCTTGCAAAGAAGTTGACGGGTCAAGTATTGGGTTTCTTTTCCCGAATGGTAGCAGAAAATGTGCGTCCTGATGAGACCACGTTTGCAGGAGTTTTAAGGGCGTGTGGTGGTGGTAACGCCAGAATCCAATGTGTGGAACAGATTCATGCTAGGATTATCCGCCATTGTTTTGGTACTAGTTTACTTGTATGTAATCCCTTGATCGATTTATATGCTAAGAACGGCTCTGTGGATTCTGCTAAAAAAGTCTTTGATAACTTGAACTTTAGGGATAGTGTTTCCTGGGTGGCTATAATTTCTGGCTTATCTCGTAATGGACGAGAAGAAGAAGCTGTTCTCCTATTCATCCAGATGCATACTTCAGGAATTTTCCCAACTCCTTATGTTTTCTCAAGCGTGATTAGTGCCTGTGCAAAAATAGAGTTATTTGAGCTGGGTGAGCAGCTGCAATGCCTTGTTTTCAAGGGAGGCTTTTCTTGTGAAACATACGTATGCAATGCTCTTGTAACATTATATTCCCGTTCAGGGAACTTCATATCTGCTGAACAAGTTTTTAATACAATGTGGCACAGGGATGGAGTATCATATAACTCATTGATATCTGGGCTAGCTCAGTGTGGATTTAGTGATAGAGCTCTGGAATTGTTCAAGAAAATGCAGAGTGACTGTATGGAACCTGATTGTGTAACAATTGCTAGTCTTTTGAGTGCCTGTGCATCAGTTGGTTATCTGTATAAGGGGAAACAACTCCACTCATATGCAATCAAAGCAGGAATGTCATCAGATATAATCCTTGAAGGGTCTCTGCTTGATCTTTATGTGAAATGCTCAGATTTGCAAACCGCCTATGAATTTTTCCTTACAACAGAGACAGAGAATGTTGTCCTGTGGAATGTGATGCTAGTTGCTTATGGGCAGCAAGATGACCTGATCGAATCGTTTCATATTTTTAAACAAATGCATGTTGAGGGTATGATTCCTAATCAATATACCTATCCGAGTATTTTGAGAACCTGTACTTCTGTTGGAGCTCTCAATTTAGGAGAACAGGTTCATACTCAAGCCATAAAAACTGGCTTTCAGTTTAATGCATATGTCTGTAGCGTGCTCATTGATATGTATGCTAAGCATGGAAAACTGGATACTGCCCTTGGAATACTCAGAAGACTCACTGAAGATGATGTTGTTTCGTGGACAGCTATGATTGCAGGGTATGCACAGCATGATTTGTTTGCTGAAGCTCTTTTACTTTTTGAAGAAATGCAAAACCGAGGGATTCGATCTGACAACATAGGATTTTCAAGTGCAATTAGTTCATGTGCTGGTATTCAAGCACTCAATCAAGGTAGACAGATTCATGCTCAGTCATGCATATCTGGTTATTCGACTGATCTTTCCGTTGGCAATGCACTCGTTACCCTTTATGCTAGATGTGGTAGAATATGGGAAGCCTACCAAGCATTTGAGAAAATTGATGTGAAAGATAATATATCATGGAATGGACTGATATCAGGGTTTGGACAGAGTGGGTACTGTGAGGAAGCACTGCAGGTATTTTCTCAGATGCATCGAGCTGGAGTAGAAGCTAATTTGTTCACATTTGGCTCTGCAGTCAGCGCTGCTGCTAATTTAGCAAATATAAAACAAGGAGAGCAGATTCATGCCATGGTGATTAAAACTGGGAACAATTCAGAAGCTGAGGTTTCCAATGCTTTAATCACGTTATACTCAAAGTGTGGTAGTGTTGATGATGCGAAGAGAGAGTTTATTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAGCATGGGCATGGTATTGAAGCACTACATCTATTTGAGCAGATGAAACAACTTGGTGTGGTGCCAAGCCATGTCACATTTGTGGGAGTACTTTCAGCTTGTAGCCATGTAGGCTTGGTAAGTGAGGGGCTTGCATACTTTGAATCCATGAGCAAAGAGCATGGACTAGTGCCTAAGCTGAGCATTATGCTTGCAAGAAAATTCATAACTGAGATGCCAATAAAACCAGATTCAATGATTTGGAGGACTCTCTTAAGTGCTTGTATAGCCAAAAAGAACACAGAAATTGGGGAGGTTGCTGCCCGTCATCTTCTAGAACTGGAACCTGAAGACTCAGCAACTTACGTTCTCATATCAAACATGTATGCAGTGGCAGGAATGTGGGGTTATAGGGATCAGGCAAGGCAATTAATGAAGGAAAGGGGTGTGAAGAAAGAGCCTGGCCGCAGTTGGATTGAGGTAAAGAATTCGGTTCATGCATTTTATGTTGGTGACCGGCTGCACCCACTTGCCAATAAGATATATGAATTCTTACGGGATTTGAATGAGCAGGCAGCTGAAATTGGTTATGTTGAGGACCGTAACAACCTCTGGAATGACATGGAGCAACAGCATAAAGATCCAACAGTGTACGTTCATAGCGAGAAATTGGCAATAGCTTTTGGCCTGATTAGCCTATCTAATACGATTCCCATACGTGTAATTAAGAATCTCCGTGTTTGTAATGATTGCCATAATTGGATCAAGCATACGTCGAAGATCTCAAAAAGAACTATCATAGTACGAGATGCATATCGTTTTCATCATTTTAAAGATGGTGTTTGCTCTTGTAAAGATTACTGGTAA

Protein Analysis

1060

Amino Acids

118.22

Weight (kDa)

6.61

Isoelectric Point (pI)

37.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 206 1.7e-06 PPR repeat family
PPR_2 PF13041 262 - 310 7.2e-08 PPR repeat family
PPR_2 PF13041 363 - 410 1.5e-08 PPR repeat family
PPR_1 PF12854 363 - 391 6.4e-06 PPR repeat
PPR PF01535 365 - 393 5.5e-08 PPR repeat
PPR_2 PF13041 464 - 510 1.5e-07 PPR repeat family
PPR_2 PF13041 565 - 612 2e-09 PPR repeat family
PPR PF01535 567 - 597 9.6e-08 PPR repeat
PPR_2 PF13041 666 - 710 8.8e-08 PPR repeat family
PPR PF01535 668 - 698 2.2e-07 PPR repeat
PPR_2 PF13041 767 - 814 4.5e-11 PPR repeat family
PPR PF01535 769 - 799 2.8e-08 PPR repeat
E_motif PF20431 872 - 932 4.4e-19 E motif
DYW_deaminase PF14432 968 - 1060 3.1e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13650
fragaria_vesca FvH4_3g25260
malus_domestica MD12G1002600.v1.1
prunus_persica Prupe.7G002600_v2.0.a1
pyrus_communis pycom12g00230
rosa_chinensis RchiOBHm_Chr5g0046881
rosa_laevigata RLG00000034452
rosa_roxburghii Rroxscaffold_1G00034510
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana Rw5G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 2053
AccB1I GGYRCC 1 cut(s) 2398
AccB7I CCANNNNNTGG 1 cut(s) 646
AccI GTMKAC 1 cut(s) 1861
AciI CCGC 4 cut(s) 161, 673, 1356, 2782
AclWI GGATC 4 cut(s) 245, 2739, 2951, 3086
AcoI YGGCCR 1 cut(s) 2779
AcsI RAATTY 5 cut(s) 879, 1364, 2537, 2803, 2864
AcuI CTGAAG 4 cut(s) 858, 1707, 1770, 2685
AfaI GTAC 6 cut(s) 688, 1531, 2045, 2427, 2969, 3120
AfeI AGCGCT 1 cut(s) 2131
AfiI CCNNNNNNNGG 2 cut(s) 646, 1038
AflII CTTAAG 1 cut(s) 2590
AflIII ACRYGT 2 cut(s) 2697, 3034
AhlI ACTAGT 2 cut(s) 689, 2503
AjnI CCWGG 2 cut(s) 794, 2776
AleI CACNNNNGTG 2 cut(s) 132, 2417
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 3 cut(s) 1150, 1546, 1623
Alw26I GTCTC 4 cut(s) 23, 581, 1320, 1376
AlwI GGATC 4 cut(s) 245, 2739, 2951, 3086
AlwNI CAGNNNCTG 1 cut(s) 2210
Aor51HI AGCGCT 1 cut(s) 2131
AoxI GGCC 2 cut(s) 2779, 3001
ApeKI GCWGC 9 cut(s) 176, 955, 958, 1429, 2132, 2135, 2636, 2836, 2891
ApoI RAATTY 5 cut(s) 879, 1364, 2537, 2803, 2864
AseI ATTAAT 1 cut(s) 2747
Asp700I GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
AspLEI GCGC 1 cut(s) 2132
AspS9I GGNCC 3 cut(s) 24, 227, 2914
AsuHPI GGTGA 4 cut(s) 443, 962, 2194, 2840
AsuNHI GCTAGC 1 cut(s) 1120
AvaII GGWCC 3 cut(s) 24, 227, 2914
BanI GGYRCC 1 cut(s) 2398
BanII GRGCYC 2 cut(s) 1150, 1546
BarI GAAGNNNNNNTAC 2 cut(s) 1743, 1775
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 1684, 2673
Bbv12I GWGCWC 3 cut(s) 1150, 1546, 1623
BbvCI CCTCAGC 1 cut(s) 2211
BbvI GCAGC 9 cut(s) 188, 945, 967, 1441, 2119, 2122, 2623, 2823, 2903
BccI CCATC 3 cut(s) 78, 1082, 3146
BceAI ACGGC 1 cut(s) 751
BcgI CGANNNNNNTGC 4 cut(s) 707, 741, 1906, 1940
BciT130I CCWGG 2 cut(s) 796, 2778
BciVI GTATCC 1 cut(s) 1648
BclI TGATCA 1 cut(s) 2322
BcoDI GTCTC 4 cut(s) 23, 581, 1320, 1376
BcuI ACTAGT 2 cut(s) 689, 2503
BfmI CTRYAG 3 cut(s) 1612, 2060, 2121
BfoI RGCGCY 1 cut(s) 2133
BfrI CTTAAG 1 cut(s) 2590
BfuAI ACCTGC 1 cut(s) 2053
BfuI GTATCC 1 cut(s) 1648
BglI GCCNNNNNGGC 1 cut(s) 2448
BglII AGATCT 1 cut(s) 3096
BlpI GCTNAGC 2 cut(s) 1638, 2516
BmcAI AGTACT 1 cut(s) 2427
Bme1390I CCNGG 2 cut(s) 796, 2778
Bme18I GGWCC 3 cut(s) 24, 227, 2914
BmgT120I GGNCC 3 cut(s) 24, 227, 2914
BmiI GGNNCC 3 cut(s) 1188, 2400, 2661
BmrFI CCNGG 2 cut(s) 796, 2778
BmrI ACTGGG 1 cut(s) 2202
BmtI GCTAGC 1 cut(s) 1124
BmuI ACTGGG 1 cut(s) 2202
BpiI GAAGAC 2 cut(s) 1684, 2673
BpmI CTGGAG 1 cut(s) 2109
Bpu10I CCTNAGC 2 cut(s) 2211, 2511
Bpu1102I GCTNAGC 2 cut(s) 1638, 2516
BpuEI CTTGAG 3 cut(s) 337, 889, 1557
Bsa29I ATCGAT 2 cut(s) 240, 717
BsaAI YACGTR 2 cut(s) 1003, 3035
BsaBI GATNNNNATC 2 cut(s) 201, 3129
BsaI GGTCTC 2 cut(s) 581, 1320
BsaJI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BsaXI ACNNNNNCTCC 3 cut(s) 31, 1548, 1578
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 1038
Bse118I RCCGGY 2 cut(s) 78, 2832
Bse1I ACTGG 6 cut(s) 1591, 1656, 2197, 2332, 2661, 3182
Bse3DI GCAATG 3 cut(s) 968, 1015, 1924
Bse8I GATNNNNATC 2 cut(s) 201, 3129
BseBI CCWGG 2 cut(s) 796, 2778
BseCI ATCGAT 2 cut(s) 240, 717
BseDI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BseGI GGATG 4 cut(s) 70, 202, 858, 1093
BseJI GATNNNNATC 2 cut(s) 201, 3129
BseLI CCNNNNNNNGG 2 cut(s) 646, 1038
BseMI GCAATG 3 cut(s) 968, 1015, 1924
BseNI ACTGG 6 cut(s) 1591, 1656, 2197, 2332, 2661, 3182
BseXI GCAGC 9 cut(s) 188, 945, 967, 1441, 2119, 2122, 2623, 2823, 2903
BseYI CCCAGC 1 cut(s) 946
BsgI GTGCAG 2 cut(s) 465, 2822
BshFI GGCC 2 cut(s) 2781, 3003
BshNI GGYRCC 1 cut(s) 2398
BshVI ATCGAT 2 cut(s) 240, 717
BsiHKAI GWGCWC 3 cut(s) 1150, 1546, 1623
BsiSI CCGG 2 cut(s) 79, 2833
BslI CCNNNNNNNGG 2 cut(s) 646, 1038
BsmAI GTCTC 4 cut(s) 23, 581, 1320, 1376
BsmI GAATGC 3 cut(s) 250, 270, 2320
BsnI GGCC 2 cut(s) 2781, 3003
Bso31I GGTCTC 2 cut(s) 581, 1320
Bsp1286I GDGCHC 3 cut(s) 1150, 1546, 1623
Bsp1720I GCTNAGC 2 cut(s) 1638, 2516
Bsp19I CCATGG 1 cut(s) 2178
BspACI CCGC 4 cut(s) 161, 673, 1356, 2782
BspANI GGCC 2 cut(s) 2781, 3003
BspDI ATCGAT 2 cut(s) 240, 717
BspLI GGNNCC 3 cut(s) 1188, 2400, 2661
BspMAI CTGCAG 2 cut(s) 2064, 2125
BspMI ACCTGC 1 cut(s) 2053
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 245, 2739, 2951, 3086
BspT107I GGYRCC 1 cut(s) 2398
BspTI CTTAAG 1 cut(s) 2590
BspTNI GGTCTC 2 cut(s) 581, 1320
BsrDI GCAATG 3 cut(s) 968, 1015, 1924
BsrFI RCCGGY 2 cut(s) 78, 2832
BsrI ACTGG 6 cut(s) 1591, 1656, 2197, 2332, 2661, 3182
BssAI RCCGGY 2 cut(s) 78, 2832
BssECI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 1663, 2178
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 796, 2778
Bst4CI ACNGT 5 cut(s) 40, 1181, 2048, 2918, 2965
Bst6I CTCTTC 1 cut(s) 2264
BstAFI CTTAAG 1 cut(s) 2590
BstAPI GCANNNNNTGC 2 cut(s) 182, 1727
BstBAI YACGTR 2 cut(s) 1003, 3035
BstC8I GCNNGC 6 cut(s) 125, 1122, 1619, 2469, 2529, 2889
BstDSI CCRYGG 1 cut(s) 2178
BstEII GGTNACC 1 cut(s) 2828
BstF5I GGATG 4 cut(s) 70, 202, 858, 1093
BstH2I RGCGCY 1 cut(s) 2133
BstHHI GCGC 1 cut(s) 2132
BstMAI GTCTC 4 cut(s) 23, 581, 1320, 1376
BstNI CCWGG 2 cut(s) 796, 2778
BstNSI RCATGY 2 cut(s) 1478, 2701
BstPI GGTNACC 1 cut(s) 2828
BstSCI CCNGG 2 cut(s) 794, 2776
BstSFI CTRYAG 3 cut(s) 1612, 2060, 2121
BstSNI TACGTA 1 cut(s) 1003
BstV1I GCAGC 9 cut(s) 188, 945, 967, 1441, 2119, 2122, 2623, 2823, 2903
BstV2I GAAGAC 2 cut(s) 1684, 2673
BstX2I RGATCY 2 cut(s) 2956, 3096
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 2 cut(s) 2956, 3096
Bsu15I ATCGAT 2 cut(s) 240, 717
BsuI GTATCC 1 cut(s) 1648
BsuRI GGCC 2 cut(s) 2781, 3003
BsuTUI ATCGAT 2 cut(s) 240, 717
BtgI CCRYGG 1 cut(s) 2178
BtsCI GGATG 4 cut(s) 70, 202, 858, 1093
BtsI GCAGTG 3 cut(s) 448, 2057, 2712
BtsIMutI CAGTG 8 cut(s) 36, 448, 1133, 1683, 2057, 2325, 2712, 2970
BveI ACCTGC 1 cut(s) 2053
Cac8I GCNNGC 6 cut(s) 125, 1122, 1619, 2469, 2529, 2889
CaiI CAGNNNCTG 1 cut(s) 2210
CfoI GCGC 1 cut(s) 2132
Cfr10I RCCGGY 2 cut(s) 78, 2832
Cfr13I GGNCC 3 cut(s) 24, 227, 2914
ClaI ATCGAT 2 cut(s) 240, 717
CseI GACGC 1 cut(s) 566
Csp6I GTAC 6 cut(s) 687, 1530, 2044, 2426, 2968, 3119
CviQI GTAC 6 cut(s) 687, 1530, 2044, 2426, 2968, 3119
DraI TTTAAA 3 cut(s) 306, 1465, 3148
DrdI GACNNNNNNGTC 1 cut(s) 407
DseDI GACNNNNNNGTC 1 cut(s) 407
EaeI YGGCCR 1 cut(s) 2779
Eam1104I CTCTTC 1 cut(s) 2264
EarI CTCTTC 1 cut(s) 2264
EciI GGCGGA 2 cut(s) 176, 662
Ecl136II GAGCTC 2 cut(s) 1148, 1544
Eco105I TACGTA 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 1663, 2178
Eco24I GRGCYC 2 cut(s) 1150, 1546
Eco31I GGTCTC 2 cut(s) 581, 1320
Eco32I GATATC 2 cut(s) 1113, 2022
Eco47I GGWCC 3 cut(s) 24, 227, 2914
Eco47III AGCGCT 1 cut(s) 2131
Eco53kI GAGCTC 2 cut(s) 1148, 1544
Eco57I CTGAAG 4 cut(s) 858, 1707, 1770, 2685
Eco91I GGTNACC 1 cut(s) 2828
EcoICRI GAGCTC 2 cut(s) 1148, 1544
EcoO109I RGGNCCY 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 2828
EcoRI GAATTC 2 cut(s) 2803, 2864
EcoRII CCWGG 2 cut(s) 794, 2776
EcoRV GATATC 2 cut(s) 1113, 2022
EcoT14I CCWWGG 2 cut(s) 1663, 2178
EcoT22I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2818, 3130
EcoT38I GRGCYC 2 cut(s) 1150, 1546
ErhI CCWWGG 2 cut(s) 1663, 2178
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FauNDI CATATG 2 cut(s) 1270, 1606
FbaI TGATCA 1 cut(s) 2322
FblI GTMKAC 1 cut(s) 1861
FokI GGATG 4 cut(s) 57, 209, 845, 1100
FriOI GRGCYC 2 cut(s) 1150, 1546
GlaI GCGC 1 cut(s) 2131
GsaI CCCAGC 1 cut(s) 950
GsuI CTGGAG 1 cut(s) 2109
HaeII RGCGCY 1 cut(s) 2133
HaeIII GGCC 2 cut(s) 2781, 3003
HapII CCGG 2 cut(s) 79, 2833
HgaI GACGC 1 cut(s) 566
HhaI GCGC 1 cut(s) 2132
Hin6I GCGC 1 cut(s) 2130
HinP1I GCGC 1 cut(s) 2130
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HpaII CCGG 2 cut(s) 79, 2833
HphI GGTGA 4 cut(s) 443, 962, 2194, 2840
Hpy166II GTNNAC 7 cut(s) 43, 528, 695, 1707, 1862, 2109, 2968
Hpy188III TCNNGA 8 cut(s) 555, 581, 862, 876, 1151, 1162, 2651, 2929
Hpy8I GTNNAC 7 cut(s) 43, 528, 695, 1707, 1862, 2109, 2968
Hpy99I CGWCG 1 cut(s) 3094
HpyAV CCTTC 3 cut(s) 215, 1304, 2746
HpyCH4III ACNGT 5 cut(s) 40, 1181, 2048, 2918, 2965
HpyCH4IV ACGT 8 cut(s) 265, 593, 1002, 2234, 2682, 2970, 3034, 3089
HpySE526I ACGT 8 cut(s) 265, 593, 1002, 2234, 2682, 2970, 3034, 3089
HspAI GCGC 1 cut(s) 2130
Ksp22I TGATCA 1 cut(s) 2322
LmnI GCTCC 2 cut(s) 1541, 2941
Lsp1109I GCAGC 9 cut(s) 188, 945, 967, 1441, 2119, 2122, 2623, 2823, 2903
MaeII ACGT 8 cut(s) 265, 593, 1002, 2234, 2682, 2970, 3034, 3089
MaeIII GTNAC 9 cut(s) 398, 626, 1018, 1175, 1198, 1927, 2410, 2828, 2918
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 2 cut(s) 2956, 3096
MhlI GDGCHC 3 cut(s) 1150, 1546, 1623
MlyI GAGTC 3 cut(s) 1674, 2578, 2663
MmeI TCCRAC 3 cut(s) 1519, 2768, 2984
Mph1103I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2818, 3130
MroXI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
MslI CAYNNNNRTG 9 cut(s) 132, 216, 863, 1003, 1086, 1269, 1628, 2346, 2417
MspA1I CMGCKG 2 cut(s) 958, 2894
MspCI CTTAAG 1 cut(s) 2590
MspI CCGG 2 cut(s) 79, 2833
MspR9I CCNGG 2 cut(s) 796, 2778
MunI CAATTG 1 cut(s) 1203
Mva1269I GAATGC 3 cut(s) 250, 270, 2320
MvaI CCWGG 2 cut(s) 796, 2778
NcoI CCATGG 1 cut(s) 2178
NdeI CATATG 2 cut(s) 1270, 1606
NheI GCTAGC 1 cut(s) 1120
NlaIV GGNNCC 3 cut(s) 1188, 2400, 2661
NmuCI GTSAC 4 cut(s) 398, 1175, 2410, 2828
NsiI ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2818, 3130
NspI RCATGY 2 cut(s) 1478, 2701
OliI CACNNNNGTG 2 cut(s) 132, 2417
PciI ACATGT 1 cut(s) 2697
PcsI WCGNNNNNNNCGW 3 cut(s) 271, 829, 2976
PctI GAATGC 3 cut(s) 250, 270, 2320
PdmI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
PflMI CCANNNNNTGG 1 cut(s) 646
PleI GAGTC 3 cut(s) 1674, 2578, 2663
PpsI GAGTC 3 cut(s) 1674, 2578, 2663
Ppu21I YACGTR 2 cut(s) 1003, 3035
PpuMI RGGWCCY 1 cut(s) 24
PscI ACATGT 1 cut(s) 2697
PshBI ATTAAT 1 cut(s) 2747
Psp124BI GAGCTC 2 cut(s) 1150, 1546
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 2 cut(s) 794, 2776
PspEI GGTNACC 1 cut(s) 2828
PspFI CCCAGC 1 cut(s) 946
PspGI CCWGG 2 cut(s) 794, 2776
PspN4I GGNNCC 3 cut(s) 1188, 2400, 2661
PspPI GGNCC 3 cut(s) 24, 227, 2914
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 2 cut(s) 2064, 2125
PstNI CAGNNNCTG 1 cut(s) 2210
PsuI RGATCY 2 cut(s) 2956, 3096
PvuII CAGCTG 2 cut(s) 958, 2894
RsaI GTAC 6 cut(s) 688, 1531, 2045, 2427, 2969, 3120
RsaNI GTAC 6 cut(s) 687, 1530, 2044, 2426, 2968, 3119
RseI CAYNNNNRTG 9 cut(s) 132, 216, 863, 1003, 1086, 1269, 1628, 2346, 2417
SacI GAGCTC 2 cut(s) 1150, 1546
Sau96I GGNCC 3 cut(s) 24, 227, 2914
ScaI AGTACT 1 cut(s) 2427
SchI GAGTC 3 cut(s) 1674, 2578, 2663
ScrFI CCNGG 2 cut(s) 796, 2778
SduI GDGCHC 3 cut(s) 1150, 1546, 1623
SfcI CTRYAG 3 cut(s) 1612, 2060, 2121
SinI GGWCC 3 cut(s) 24, 227, 2914
SmiMI CAYNNNNRTG 9 cut(s) 132, 216, 863, 1003, 1086, 1269, 1628, 2346, 2417
SmlI CTYRAG 4 cut(s) 316, 904, 1572, 2590
SmoI CTYRAG 4 cut(s) 316, 904, 1572, 2590
SnaBI TACGTA 1 cut(s) 1003
SpeI ACTAGT 2 cut(s) 689, 2503
SsiI CCGC 4 cut(s) 161, 673, 1356, 2782
SstI GAGCTC 2 cut(s) 1150, 1546
StyD4I CCNGG 2 cut(s) 794, 2776
StyI CCWWGG 2 cut(s) 1663, 2178
TaaI ACNGT 5 cut(s) 40, 1181, 2048, 2918, 2965
TaiI ACGT 8 cut(s) 268, 596, 1005, 2237, 2685, 2973, 3037, 3092
TaqI TCGA 8 cut(s) 240, 459, 717, 1446, 1792, 1898, 2083, 3092
TatI WGTACW 2 cut(s) 1529, 2425
TauI GCSGC 1 cut(s) 2784
TscAI CASTG 8 cut(s) 43, 448, 1133, 1690, 2064, 2332, 2712, 2970
TseFI GTSAC 4 cut(s) 398, 1175, 2410, 2828
TseI GCWGC 9 cut(s) 176, 955, 958, 1429, 2132, 2135, 2636, 2836, 2891
Tsp45I GTSAC 4 cut(s) 398, 1175, 2410, 2828
TspGWI ACGGA 2 cut(s) 1900, 3041
TspRI CASTG 8 cut(s) 43, 448, 1133, 1690, 2064, 2332, 2712, 2970
Van91I CCANNNNNTGG 1 cut(s) 646
Vha464I CTTAAG 1 cut(s) 2590
VpaK11BI GGWCC 3 cut(s) 24, 227, 2914
VspI ATTAAT 1 cut(s) 2747
XapI RAATTY 5 cut(s) 879, 1364, 2537, 2803, 2864
XbaI TCTAGA 1 cut(s) 2650
XceI RCATGY 2 cut(s) 1478, 2701
XmiI GTMKAC 1 cut(s) 1861
XmnI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
ZrmI AGTACT 1 cut(s) 2427
Zsp2I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2818, 3130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.