Rh5BG320800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0016595
Physical Location & Seq
Forward (+)
42960291 .. 42963587
3297 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG320800.1

Sequence Viewer

Length: 3225 bp
ATGAGTGGGTGTCTCAGGTCCTTGATGCTCACTGTAAACCAATGCCACAACCCTATGTTCTTCCATCGCTACCGGCTTCATTTCAGAACCAAACCCAAACCCACCTCAGTTATCAATCTTCGCAAGCACTTTTGTGGGAGTGTAGTCAGTTTGAGCTTGGCGGAATATAGTAATGCAGCCAATGCTCGTGTTTCGGATGATTATCTAGTTCATCAAAATGAAGGTGGTCCAAAGGGGATCGATTTGTTGCATTCTATGGAAGCACGTTGCATTCGTGCCAATTCTCAGACGTATATTTGGCTTTTAAAAGGGTGCTTGAGTTCTGGGTCTTTGTTAGATGCTAGGAAACTTCACTCTAGGATTTTGAAGTTGGGGTTTGGTGGAGAATATGAAATAAGTGACCTGTTTGTTGGTGTTTATCTTGCAAATGATGACTCATGCAGCGCAGTCAAGGTTTTCGATGATCTACCATATAGAAGTTTATTCTCATGGAATAACATAATTCATGGGTTTCTTGCAAAGAAGTTGACGGGTCAAGTATTGGGTTTCTTTTCCCGAATGGTAGCAGAAAATGTGCGTCCTGATGAGACCACGTTTGCAGGAGTTTTAAGGGCGTGTGGTGGTGGTAATGCCAGTATCCAATATGTGGAACAGATTCATGCTAGGATTATCCGCCATTGTTTTGGTACTAGTTTACTTGTATGTAATCCCTTGATCGATTTGTATGCTAAGAACAGCTCTGTGGATTCTGCTAAAAAAGTCTTTGATAAATTGAATTTTAGGGATAGTGTTTCCTGGGTGGCTATAATTTCTGGCTTATCTCGTAATGGACGAGAAGAAGAAGCTGTTCTCCTATTCATCCAGATGCATACGTCAGGAATTTTCCCAACTCCTTATGTTTTCTCAAGCGTGATTAGTGCCTGTGCAAAAATAGAGTTATTTGAGCTGGGTGAGCAGCTGCAATGCCTTGTTTTCAAGGGAGGCTTTTCTTGTGAAACATACGTATGCAATGCTCTTGTAACATTATATTCCCGTTCAGGGAACTTCATATCTGCTGAACAAGTTTTCAATACAATGTGGTACAGGGATGGAGTATCATATAACTCATTGATATCTGGGCTAGCTCAGTGTGGGTTTAGTGATAGGGCTCTGGAATTGTTCAAGAAAATGCAGAGTGACTGTATGGAACCTGACTGTGTAACAATTGCTAGTCTTTTGAGTGCCTGTGCATCAGTTGGTTATCTGTATAAGGGGAAACAACTCCACTCATATGCAATTAAAGCAGGAATGTCATCAGATATAATCCTCGAAGGGTCTCTGCTTGATCTTTATGTGAAATGCTCAGATTTGCAAACCGCCTATGAATTTTTCCTTACAACAGAGACAGAGAATGTTGTCCTGTGGAATGTGATGCTAGTTGCTTATGGGCAGCAAGATGACCTGATCGAATCGTTTCATATTTTTAAACAAATGCATGTTGAGGGTATGATTCCTAATCAATATACCTATCCGAGTATTTTGAGAACCTGTACTTCTGTTGGAGCTCTCAATTTAGGAGAACAGGTTCATACTCAAGCCATAAAAACTGGCTTTCAGTTTAATGCATATGTCTGTAGCGTGCTCATTGATATGTATGCTAAGCATGGAAAACTGGATACTGCCCTTGGAATACTCAGAAGACTCACTGAAGATGATGTTGTTTCGTGGACAGCTATGATTGCAGGGTATGCACAGCATGATTTGTTTGCTGAAGCTCTTTTACTTTTTGAAGAAATGCAAAACCGAGGGATTCGATCTGACAACATAGGATTTTCAAGTGCAATTAGTTCATGTGCTGGTATTCAAGCACTCAATCAAGGTAGACAGATTCATGCTCAGTCATGCATATCTGGTTATTCGACTGATCTTTCCGTTGGCAATGCACTCGTTACCCTTTATGCTAGATGTGGTAGAATATGGGAAGCCTACCAAGCATTTGAGAAAATTGATGTGAAAGATAATATATCATGGAATGGACTGATATCAGGGTTTGGACAGAGTGGGTACTGTGAGGAAGCACTGCAGTTATTTTCTCAGATGCATCGAGCTGGAGTAGAAGCTAATTTGTTCACATTTGGCTCTGCAGTCAGCGCTGCTGCTAATTTAGCAAATATAAAACAAGGAGAGCAGATTCATGCCATGGTGATAAAAACTGGGAACAATTCAGAAGCTGAGGTTTCCAATGCTTTAATCACATTATACTCAAAGTGTGGTAGTGTTGATGATGCAAAGAGAGAGTTTATTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACCGGCTATTCTCAGCATGGGCATGGTATTGAAGCACTACATCTATTTGAGCAGATGAAACAACTTGGTGTGGTGCCAAGCCATGTCACATTTGTGGGAGTACTTTCAGCTTGTAGCCATGTAGGCTTGGTAAGTGAGGGGCTTGCATACTTTGAATCCATGAGCAAAGAGCATGGACTACTGCCTAAGGCTGAGCATTATGCTTGTGTTGTTGATCTTCTTAGTCGAGCTGGTTCTTTGAGCCAGGCAAGAAAATTCATAACTGAGATGCCAATAAAACCAGATTCAATGATTTGGAGGACTCTCTTAAGTGCTTGTATAGCTAAAAAGAACACAGAAATTGGGGAGGTTGCTGCCCGTCATCTTCTAGAACTGGAACCTGAAGACTCAGCAACTTACGTTCTCATATCAAACATGTATGCAGTGGCAGGAATGTGGGGTTATAGGGATCAGGCAAGGCAATTAATGAAGGAAAGGGGTGTGAAGAAAGAGCCTGGCCGCAGTTGGATTGAGGTAAAGAATTCGGTTCATGCATTCTATGTTGGTGACCGGCTGCACCCACTTGCCAATAAGATATATGAATTCTTAGGGGATTTGAATGAGCGGGCAGCTGAAATTGGTTATGTTGAGGACCGTAACAACCTCTGGAATGACATGGAGCAACAGCATAAAGATCCAACAGTGTACATTCATAGCGAGAAATTGGCAATAGCTTTTGGCCTGATTAGCCTATCTAATACGATTCCCATACGTGTAATTAAGAATCTCCGTGTTTGTAATGATTGCCATAATTGGATCAAGCATACGTCGAAGATCTCAAAAAGAACTATCATAGTACGAGATGCATATCGTTTTCATCATTTTAAAGATGGTGTTTGCTCTTGTAAAGATTACTGGTAA

Protein Analysis

1074

Amino Acids

119.84

Weight (kDa)

6.54

Isoelectric Point (pI)

38.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 161 - 206 1.7e-06 PPR repeat family
PPR_2 PF13041 262 - 310 7.3e-08 PPR repeat family
PPR_2 PF13041 363 - 410 1.5e-08 PPR repeat family
PPR_1 PF12854 363 - 391 8.5e-06 PPR repeat
PPR PF01535 365 - 393 5.6e-08 PPR repeat
PPR_2 PF13041 464 - 510 1.5e-07 PPR repeat family
PPR_2 PF13041 565 - 612 2e-09 PPR repeat family
PPR PF01535 567 - 597 9.8e-08 PPR repeat
PPR_2 PF13041 666 - 710 4.2e-08 PPR repeat family
PPR PF01535 668 - 698 1.4e-07 PPR repeat
PPR_2 PF13041 767 - 814 4.6e-11 PPR repeat family
PPR PF01535 769 - 799 2.8e-08 PPR repeat
E_motif PF20431 886 - 946 4.5e-19 E motif
DYW_deaminase PF14432 982 - 1074 4e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13650
fragaria_vesca FvH4_3g25260
malus_domestica MD12G1002600.v1.1
prunus_persica Prupe.7G002600_v2.0.a1
pyrus_communis pycom12g00230
rosa_chinensis RchiOBHm_Chr5g0046881
rosa_laevigata RLG00000034452
rosa_roxburghii Rroxscaffold_1G00034510
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana Rw5G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2398
AccB7I CCANNNNNTGG 1 cut(s) 646
AccBSI CCGCTC 1 cut(s) 2929
AccI GTMKAC 1 cut(s) 1861
AciI CCGC 5 cut(s) 161, 673, 1356, 2824, 2929
AclWI GGATC 4 cut(s) 245, 2781, 2993, 3128
AcoI YGGCCR 1 cut(s) 2821
AcsI RAATTY 6 cut(s) 775, 879, 1364, 2579, 2845, 2906
AcuI CTGAAG 3 cut(s) 1707, 1770, 2727
AfaI GTAC 7 cut(s) 688, 1082, 1531, 2045, 2427, 3011, 3162
AfeI AGCGCT 1 cut(s) 2131
AfiI CCNNNNNNNGG 2 cut(s) 646, 1038
AflII CTTAAG 1 cut(s) 2632
AflIII ACRYGT 2 cut(s) 2739, 3076
AhlI ACTAGT 1 cut(s) 689
AjnI CCWGG 3 cut(s) 794, 2568, 2818
AleI CACNNNNGTG 2 cut(s) 132, 2417
AloI GAACNNNNNNTCC 2 cut(s) 1548, 1580
Alw21I GWGCWC 2 cut(s) 1546, 1623
Alw26I GTCTC 4 cut(s) 17, 581, 1320, 1376
AlwI GGATC 4 cut(s) 245, 2781, 2993, 3128
AlwNI CAGNNNCTG 1 cut(s) 2210
Aor51HI AGCGCT 1 cut(s) 2131
AoxI GGCC 2 cut(s) 2821, 3043
ApoI RAATTY 6 cut(s) 775, 879, 1364, 2579, 2845, 2906
AseI ATTAAT 1 cut(s) 2789
Asp700I GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
AspLEI GCGC 2 cut(s) 446, 2132
AspS9I GGNCC 3 cut(s) 18, 227, 2956
AsuHPI GGTGA 4 cut(s) 962, 2194, 2317, 2882
AsuNHI GCTAGC 1 cut(s) 1120
AvaII GGWCC 3 cut(s) 18, 227, 2956
AxyI CCTNAGG 1 cut(s) 2511
BanI GGYRCC 1 cut(s) 2398
BanII GRGCYC 2 cut(s) 1150, 1546
BarI GAAGNNNNNNTAC 2 cut(s) 1743, 1775
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 1684, 2715
Bbv12I GWGCWC 2 cut(s) 1546, 1623
BbvCI CCTCAGC 1 cut(s) 2211
BccI CCATC 3 cut(s) 72, 1082, 3188
BcgI CGANNNNNNTGC 4 cut(s) 707, 741, 1906, 1940
BciT130I CCWGG 3 cut(s) 796, 2570, 2820
BciVI GTATCC 2 cut(s) 647, 1648
BclI TGATCA 1 cut(s) 2322
BcoDI GTCTC 4 cut(s) 17, 581, 1320, 1376
BcuI ACTAGT 1 cut(s) 689
BfmI CTRYAG 3 cut(s) 1612, 2060, 2121
BfoI RGCGCY 1 cut(s) 2133
BfrI CTTAAG 1 cut(s) 2632
BfuI GTATCC 2 cut(s) 647, 1648
BglI GCCNNNNNGGC 1 cut(s) 2448
BglII AGATCT 1 cut(s) 3138
BlpI GCTNAGC 2 cut(s) 1638, 2517
BmcAI AGTACT 1 cut(s) 2427
Bme1390I CCNGG 3 cut(s) 796, 2570, 2820
Bme18I GGWCC 3 cut(s) 18, 227, 2956
BmgT120I GGNCC 3 cut(s) 18, 227, 2956
BmiI GGNNCC 3 cut(s) 1188, 2400, 2703
BmrFI CCNGG 3 cut(s) 796, 2570, 2820
BmrI ACTGGG 1 cut(s) 2202
BmtI GCTAGC 1 cut(s) 1124
BmuI ACTGGG 1 cut(s) 2202
BpiI GAAGAC 2 cut(s) 1684, 2715
BpmI CTGGAG 1 cut(s) 2109
Bpu10I CCTNAGC 1 cut(s) 2211
Bpu1102I GCTNAGC 2 cut(s) 1638, 2517
BpuEI CTTGAG 3 cut(s) 337, 889, 1557
Bsa29I ATCGAT 2 cut(s) 240, 717
BsaAI YACGTR 2 cut(s) 1003, 3077
BsaBI GATNNNNATC 2 cut(s) 201, 3171
BsaI GGTCTC 2 cut(s) 581, 1320
BsaJI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BsaXI ACNNNNNCTCC 2 cut(s) 1548, 1578
Bsc4I CCNNNNNNNGG 2 cut(s) 646, 1038
Bse118I RCCGGY 3 cut(s) 72, 2327, 2874
Bse1I ACTGG 6 cut(s) 633, 1591, 1656, 2197, 2703, 3224
Bse21I CCTNAGG 1 cut(s) 2511
Bse3DI GCAATG 3 cut(s) 968, 1015, 1924
Bse8I GATNNNNATC 2 cut(s) 201, 3171
BseBI CCWGG 3 cut(s) 796, 2570, 2820
BseCI ATCGAT 2 cut(s) 240, 717
BseDI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BseGI GGATG 3 cut(s) 202, 858, 1093
BseJI GATNNNNATC 2 cut(s) 201, 3171
BseLI CCNNNNNNNGG 2 cut(s) 646, 1038
BseMI GCAATG 3 cut(s) 968, 1015, 1924
BseNI ACTGG 6 cut(s) 633, 1591, 1656, 2197, 2703, 3224
BseYI CCCAGC 1 cut(s) 946
BsgI GTGCAG 1 cut(s) 2864
BshFI GGCC 2 cut(s) 2823, 3045
BshNI GGYRCC 1 cut(s) 2398
BshVI ATCGAT 2 cut(s) 240, 717
BsiHKAI GWGCWC 2 cut(s) 1546, 1623
BsiSI CCGG 3 cut(s) 73, 2328, 2875
BslI CCNNNNNNNGG 2 cut(s) 646, 1038
BsmAI GTCTC 4 cut(s) 17, 581, 1320, 1376
BsmI GAATGC 4 cut(s) 250, 270, 2320, 2858
BsnI GGCC 2 cut(s) 2823, 3045
Bso31I GGTCTC 2 cut(s) 581, 1320
Bsp1286I GDGCHC 3 cut(s) 1150, 1546, 1623
Bsp1407I TGTACA 1 cut(s) 3009
Bsp1720I GCTNAGC 2 cut(s) 1638, 2517
Bsp19I CCATGG 1 cut(s) 2178
BspACI CCGC 5 cut(s) 161, 673, 1356, 2824, 2929
BspANI GGCC 2 cut(s) 2823, 3045
BspDI ATCGAT 2 cut(s) 240, 717
BspLI GGNNCC 3 cut(s) 1188, 2400, 2703
BspMAI CTGCAG 2 cut(s) 2064, 2125
BspOI GCTAGC 1 cut(s) 1124
BspPI GGATC 4 cut(s) 245, 2781, 2993, 3128
BspT107I GGYRCC 1 cut(s) 2398
BspTI CTTAAG 1 cut(s) 2632
BspTNI GGTCTC 2 cut(s) 581, 1320
BsrBI CCGCTC 1 cut(s) 2929
BsrDI GCAATG 3 cut(s) 968, 1015, 1924
BsrFI RCCGGY 3 cut(s) 72, 2327, 2874
BsrGI TGTACA 1 cut(s) 3009
BsrI ACTGG 6 cut(s) 633, 1591, 1656, 2197, 2703, 3224
BssAI RCCGGY 3 cut(s) 72, 2327, 2874
BssECI CCNNGG 4 cut(s) 795, 1663, 1783, 2178
BssSI CACGAG 1 cut(s) 186
BssT1I CCWWGG 2 cut(s) 1663, 2178
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 3 cut(s) 796, 2570, 2820
Bst4CI ACNGT 6 cut(s) 34, 1181, 1196, 2048, 2960, 3007
BstAFI CTTAAG 1 cut(s) 2632
BstAPI GCANNNNNTGC 2 cut(s) 182, 1727
BstAUI TGTACA 1 cut(s) 3009
BstBAI YACGTR 2 cut(s) 1003, 3077
BstC8I GCNNGC 5 cut(s) 125, 1122, 1619, 2469, 2931
BstDSI CCRYGG 1 cut(s) 2178
BstEII GGTNACC 1 cut(s) 2870
BstF5I GGATG 3 cut(s) 202, 858, 1093
BstH2I RGCGCY 1 cut(s) 2133
BstHHI GCGC 2 cut(s) 446, 2132
BstMAI GTCTC 4 cut(s) 17, 581, 1320, 1376
BstNI CCWGG 3 cut(s) 796, 2570, 2820
BstNSI RCATGY 2 cut(s) 1478, 2743
BstPI GGTNACC 1 cut(s) 2870
BstSCI CCNGG 3 cut(s) 794, 2568, 2818
BstSFI CTRYAG 3 cut(s) 1612, 2060, 2121
BstSNI TACGTA 1 cut(s) 1003
BstV2I GAAGAC 2 cut(s) 1684, 2715
BstX2I RGATCY 2 cut(s) 2998, 3138
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 2 cut(s) 2998, 3138
Bsu15I ATCGAT 2 cut(s) 240, 717
Bsu36I CCTNAGG 1 cut(s) 2511
BsuI GTATCC 2 cut(s) 647, 1648
BsuRI GGCC 2 cut(s) 2823, 3045
BsuTUI ATCGAT 2 cut(s) 240, 717
BtgI CCRYGG 1 cut(s) 2178
BtgZI GCGATG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 202, 858, 1093
BtsI GCAGTG 2 cut(s) 2057, 2754
BtsIMutI CAGTG 6 cut(s) 30, 1133, 1683, 2057, 2754, 3012
Cac8I GCNNGC 5 cut(s) 125, 1122, 1619, 2469, 2931
CaiI CAGNNNCTG 1 cut(s) 2210
CfoI GCGC 2 cut(s) 446, 2132
Cfr10I RCCGGY 3 cut(s) 72, 2327, 2874
Cfr13I GGNCC 3 cut(s) 18, 227, 2956
ClaI ATCGAT 2 cut(s) 240, 717
CseI GACGC 1 cut(s) 566
Csp6I GTAC 7 cut(s) 687, 1081, 1530, 2044, 2426, 3010, 3161
CviQI GTAC 7 cut(s) 687, 1081, 1530, 2044, 2426, 3010, 3161
DraI TTTAAA 3 cut(s) 306, 1465, 3190
EaeI YGGCCR 1 cut(s) 2821
EciI GGCGGA 2 cut(s) 176, 662
Ecl136II GAGCTC 1 cut(s) 1544
Eco105I TACGTA 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 1663, 2178
Eco24I GRGCYC 2 cut(s) 1150, 1546
Eco31I GGTCTC 2 cut(s) 581, 1320
Eco32I GATATC 2 cut(s) 1113, 2022
Eco47I GGWCC 3 cut(s) 18, 227, 2956
Eco47III AGCGCT 1 cut(s) 2131
Eco53kI GAGCTC 1 cut(s) 1544
Eco57I CTGAAG 3 cut(s) 1707, 1770, 2727
Eco81I CCTNAGG 1 cut(s) 2511
Eco91I GGTNACC 1 cut(s) 2870
EcoICRI GAGCTC 1 cut(s) 1544
EcoO109I RGGNCCY 1 cut(s) 18
EcoO65I GGTNACC 1 cut(s) 2870
EcoRI GAATTC 2 cut(s) 2845, 2906
EcoRII CCWGG 3 cut(s) 794, 2568, 2818
EcoRV GATATC 2 cut(s) 1113, 2022
EcoT14I CCWWGG 2 cut(s) 1663, 2178
EcoT22I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2860, 3172
EcoT38I GRGCYC 2 cut(s) 1150, 1546
ErhI CCWWGG 2 cut(s) 1663, 2178
FalI AAGNNNNNCTT 2 cut(s) 968, 1000
FauI CCCGC 1 cut(s) 2922
FauNDI CATATG 2 cut(s) 1270, 1606
FbaI TGATCA 1 cut(s) 2322
FblI GTMKAC 1 cut(s) 1861
FokI GGATG 3 cut(s) 209, 845, 1100
FriOI GRGCYC 2 cut(s) 1150, 1546
GlaI GCGC 2 cut(s) 445, 2131
GsaI CCCAGC 1 cut(s) 950
GsuI CTGGAG 1 cut(s) 2109
HaeII RGCGCY 1 cut(s) 2133
HaeIII GGCC 2 cut(s) 2823, 3045
HapII CCGG 3 cut(s) 73, 2328, 2875
HgaI GACGC 1 cut(s) 566
HhaI GCGC 2 cut(s) 446, 2132
Hin6I GCGC 2 cut(s) 444, 2130
HinP1I GCGC 2 cut(s) 444, 2130
HincII GTYRAC 1 cut(s) 528
HindII GTYRAC 1 cut(s) 528
HpaII CCGG 3 cut(s) 73, 2328, 2875
HphI GGTGA 4 cut(s) 962, 2194, 2317, 2882
Hpy166II GTNNAC 7 cut(s) 37, 528, 695, 1707, 1862, 2109, 3010
Hpy188III TCNNGA 8 cut(s) 555, 581, 862, 876, 1151, 1162, 2693, 2971
Hpy8I GTNNAC 7 cut(s) 37, 528, 695, 1707, 1862, 2109, 3010
Hpy99I CGWCG 1 cut(s) 3136
HpyAV CCTTC 3 cut(s) 215, 1304, 2788
HpyCH4III ACNGT 6 cut(s) 34, 1181, 1196, 2048, 2960, 3007
HpyCH4IV ACGT 8 cut(s) 265, 290, 593, 872, 1002, 2724, 3076, 3131
HpySE526I ACGT 8 cut(s) 265, 290, 593, 872, 1002, 2724, 3076, 3131
HspAI GCGC 2 cut(s) 444, 2130
Ksp22I TGATCA 1 cut(s) 2322
LmnI GCTCC 2 cut(s) 1541, 2983
MaeII ACGT 8 cut(s) 265, 290, 593, 872, 1002, 2724, 3076, 3131
MaeIII GTNAC 8 cut(s) 398, 1018, 1175, 1198, 1927, 2410, 2870, 2960
MbiI CCGCTC 1 cut(s) 2929
MfeI CAATTG 1 cut(s) 1203
MflI RGATCY 2 cut(s) 2998, 3138
MhlI GDGCHC 3 cut(s) 1150, 1546, 1623
MlyI GAGTC 4 cut(s) 428, 1674, 2620, 2705
MmeI TCCRAC 3 cut(s) 1519, 2810, 3026
Mph1103I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2860, 3172
MroXI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
MslI CAYNNNNRTG 8 cut(s) 132, 216, 863, 1003, 1269, 1628, 2346, 2417
MspA1I CMGCKG 2 cut(s) 958, 2936
MspCI CTTAAG 1 cut(s) 2632
MspI CCGG 3 cut(s) 73, 2328, 2875
MspR9I CCNGG 3 cut(s) 796, 2570, 2820
MunI CAATTG 1 cut(s) 1203
Mva1269I GAATGC 4 cut(s) 250, 270, 2320, 2858
MvaI CCWGG 3 cut(s) 796, 2570, 2820
NcoI CCATGG 1 cut(s) 2178
NdeI CATATG 2 cut(s) 1270, 1606
NheI GCTAGC 1 cut(s) 1120
NlaIV GGNNCC 3 cut(s) 1188, 2400, 2703
NmuCI GTSAC 4 cut(s) 398, 1175, 2410, 2870
NsiI ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2860, 3172
NspI RCATGY 2 cut(s) 1478, 2743
OliI CACNNNNGTG 2 cut(s) 132, 2417
PciI ACATGT 1 cut(s) 2739
PcsI WCGNNNNNNNCGW 2 cut(s) 271, 829
PctI GAATGC 4 cut(s) 250, 270, 2320, 2858
PdmI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
PflMI CCANNNNNTGG 1 cut(s) 646
PleI GAGTC 4 cut(s) 428, 1674, 2620, 2705
PpsI GAGTC 4 cut(s) 428, 1674, 2620, 2705
Ppu21I YACGTR 2 cut(s) 1003, 3077
PpuMI RGGWCCY 1 cut(s) 18
PscI ACATGT 1 cut(s) 2739
PshBI ATTAAT 1 cut(s) 2789
Psp124BI GAGCTC 1 cut(s) 1546
Psp5II RGGWCCY 1 cut(s) 18
Psp6I CCWGG 3 cut(s) 794, 2568, 2818
PspEI GGTNACC 1 cut(s) 2870
PspFI CCCAGC 1 cut(s) 946
PspGI CCWGG 3 cut(s) 794, 2568, 2818
PspN4I GGNNCC 3 cut(s) 1188, 2400, 2703
PspPI GGNCC 3 cut(s) 18, 227, 2956
PspPPI RGGWCCY 1 cut(s) 18
PstI CTGCAG 2 cut(s) 2064, 2125
PstNI CAGNNNCTG 1 cut(s) 2210
PsuI RGATCY 2 cut(s) 2998, 3138
PvuII CAGCTG 2 cut(s) 958, 2936
RsaI GTAC 7 cut(s) 688, 1082, 1531, 2045, 2427, 3011, 3162
RsaNI GTAC 7 cut(s) 687, 1081, 1530, 2044, 2426, 3010, 3161
RseI CAYNNNNRTG 8 cut(s) 132, 216, 863, 1003, 1269, 1628, 2346, 2417
SacI GAGCTC 1 cut(s) 1546
Sau96I GGNCC 3 cut(s) 18, 227, 2956
ScaI AGTACT 1 cut(s) 2427
SchI GAGTC 4 cut(s) 428, 1674, 2620, 2705
ScrFI CCNGG 3 cut(s) 796, 2570, 2820
SduI GDGCHC 3 cut(s) 1150, 1546, 1623
SfcI CTRYAG 3 cut(s) 1612, 2060, 2121
SinI GGWCC 3 cut(s) 18, 227, 2956
SmiMI CAYNNNNRTG 8 cut(s) 132, 216, 863, 1003, 1269, 1628, 2346, 2417
SmlI CTYRAG 4 cut(s) 316, 904, 1572, 2632
SmoI CTYRAG 4 cut(s) 316, 904, 1572, 2632
SnaBI TACGTA 1 cut(s) 1003
SpeI ACTAGT 1 cut(s) 689
SsiI CCGC 5 cut(s) 161, 673, 1356, 2824, 2929
SstI GAGCTC 1 cut(s) 1546
StyD4I CCNGG 3 cut(s) 794, 2568, 2818
StyI CCWWGG 2 cut(s) 1663, 2178
TaaI ACNGT 6 cut(s) 34, 1181, 1196, 2048, 2960, 3007
TaiI ACGT 8 cut(s) 268, 293, 596, 875, 1005, 2727, 3079, 3134
TatI WGTACW 3 cut(s) 1529, 2425, 3009
TauI GCSGC 1 cut(s) 2826
TscAI CASTG 6 cut(s) 37, 1133, 1690, 2064, 2754, 3012
TseFI GTSAC 4 cut(s) 398, 1175, 2410, 2870
Tsp45I GTSAC 4 cut(s) 398, 1175, 2410, 2870
TspGWI ACGGA 2 cut(s) 1900, 3083
TspRI CASTG 6 cut(s) 37, 1133, 1690, 2064, 2754, 3012
Van91I CCANNNNNTGG 1 cut(s) 646
Vha464I CTTAAG 1 cut(s) 2632
VpaK11BI GGWCC 3 cut(s) 18, 227, 2956
VspI ATTAAT 1 cut(s) 2789
XapI RAATTY 6 cut(s) 775, 879, 1364, 2579, 2845, 2906
XbaI TCTAGA 1 cut(s) 2692
XceI RCATGY 2 cut(s) 1478, 2743
XmiI GTMKAC 1 cut(s) 1861
XmnI GAANNNNTTC 4 cut(s) 654, 846, 1452, 1563
ZrmI AGTACT 1 cut(s) 2427
Zsp2I ATGCAT 7 cut(s) 870, 1476, 1606, 1886, 2082, 2860, 3172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.