Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0016595

Physical Location & Seq

Genomic Coordinates Reverse (-)

307738 .. 311107

Gene Structure 3370 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g00230.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3261 bp

ATGAGTGGCTCTGGCTGTCTCAAATCTTTGATGCTCGCTCTGACTCTCCACCAATTCCCCGACCCTCTCTTCTTCCATCGCTTCCCGCCGCTTTATTTCACAACCAAACCCAAACCCAAACCCACCTCAAGTTTCAATCTTCACAAGCGCTTTCGTGGGAGTGTCATCCGGTTGAGCTCGCCAGAAGAATATACTAGTAATGCAGCAGCAGCCAATGCCCCTGTCCTCGACGACTTTCTACTTCATCAAAATGAAGGTAATAATCCAAAGGGGATTGAATTCCTGCATTCCATGGAAACACGTAGTGTTCGTGCTAATTGTCAGACATATATTTGGCTTTTAAAAGGCTGCTCCAATTCCGGTTCCTTGTTAGATTCTAAGAAGCTTCACTCTAGGATTCTGAAGCTGGGTTTTGATGCTGAACATGTAATATGCGATGGCCTTATTGATGCTTACCTTGCTGCTGGTGATTTAGATGGCGCAGTTAGGGTTTTTGATGATGTGCCCTACAGAAGTTTGTTTTCATGGAATAACATAATTCAAGTGTTTCTTGCAAACAAGTTGACAGGTCAAGTATTGGATTTCTTTTCCCGAATGTTAGCAGATAATGTGCATCCTGATGAGACCACGTTTTCCAGGGTTTTAAGGGCATGTGGTGGCAGTAATGTTCATCTTCAATATGTCAAACAGATTCATGCTAGGGTTATCTGTCATGGTTTCGGTACTAGTTTGCTTGTGTGTAATCCCTTGATTGATTTGTATGCCAAAAACGGGTCTGTAGATGCTGCTAAAAAGGTCTTTGATAAATTGTATATAAGGGATAGCGTTTCCTGGGTTGCCATGATTTCTGGTTTATCTCAGAATGGACGTGAAGAAGAAGCAATTCTCTTATTCATTGGGATGCAAACGTCAGATATTTTACCCACTCCTTATGTGTTCTCCAGCGTGCTAAGTGCCTGCACCAAAATAGAGTTATTTAAGATGGGAGAGCAACTGCATGGCCTTATTTTCAAGGGGGGTTTTTCTTGTGAAACATATGTATGCAATGCACTTGTGACGTTATATTCCCGCTCAGGGAACTTTATATCTGCTGAACAAATTTTCAAAACAATGTGGCAGAGGGATGCAGTATCTTATAACTCACTGATCTCTGGGTTAGCTCAGTGCGGATTTAGTGATAGAGCATTGGAATTGTTCAAAAGAATGCAGATTGATTGTTTGAGACCAGACTGTGTTACAATTGCTAGTCTTTTGAGTGCATGCGCGGAAATTGGTGCTCTGCAGAAGGGAAAACAACTCCACTCACTTGCAATAAAAGCGGGAATGTCATCAGATATTATCCTTGAAGGTTCTCTGCTTGATCTTTATGTGAAATGCTCAGATGTGCAAACTGCCTATGAATTTTTCCTTACAACAGAGACAGAAAATGTGGTCCTGTGGAATGTGATGCTAGTTGCTTATGGTCAGTTGGATGATCTGGATCAATCATTTCGGATATTTAGGCAAATGCATGTTGAGGGCATGATTCCTAATCAGTACACCTACCCTAGTATTCTAAGAACCTGTACCTCTGTTGGAGCTCTCAATTTGGGAGAGCAGATACATACTCAAGTCATAAAAACTGGTTTTCATTTTAATGTTTATGTCTGTAGTGTGCTCATCGATATGTATGCTAAGCATGGGGAGCTGGATACTGCCCTGAGAATTCTCAGACGACTCACTGCAGATGACATCGTCTCATGGACAGCTATGATAGCCGGGTATGCACAGCATGATCTATTTTCTGAATCTCTGATACTTTTTGAAGAAATGCAAAGGCGAGGGATTCAGTCTGACAACATAGGATTTTCAAGTGCAATAAGTGCATGTGCTGGTATTCAAGCACTTAGTCAAGGAAGACAAATTCATGCTCAGGCGTGTGTCTTTGGTTATTCAGATGATCTTTCAGTTGGAAATGCACTTGTTACCCTTTACTCTAGATGTGGTAGAATACAGGAAGCATACCGAGCATTTGAGGCAACTGATTCTAAAGATAATATGTCATGGAATGGATTGATATCTGGGTTTGCACAGAGTGGGTACTATGAGGAAGCGCTGCAAGTATTTGCTTGGATGAATAAAGCTGGAATGGAAGCTAACATGTTCACATTTGGCTCTGCAGTTAGTGCTGCTGCTAACTTAGCAAATATAAAACAAGGGCAGCAGATCCATGCCACAATTATAAAAACTGGCAGCAATTCCGAAACTGAGGTTTCCAATGCGTTAATCACATTGTATTCAAAGTGTGGTAGCATTAATGATGCCAAGAGAGAGTTTTCTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAACATGGGCGTGGTATTGAATCAATACACCTATTTGAGCGGATGAAACAACTTGGTATAGCACCAAGCCATGTCACATTTGTGGGGGTACTTACAGCCTGTAGCCATATAGGCTTGGTAAATGAAGGGCTTGGCTACTTTGAATCCATGAGAAAAGAGCATGGGTTAGTGCCTAAGCCTGAACACTATGCTTGCGTTGTTGATCTTCTAGGTAGAGCTGGTTCTCTGAGCCGTGCCAGAAAGTTTATAGAGGAGATGCCAGTGAAACCAGATGCAATGATTTGGAGGACTCTTTTAAGTGCTTGCATAACTCGCAAGAACACTGAAATTGGAGAGTTTGCTGCCAATCATCTTCTAGAACTGGAACCTGAAGACTCAGCAACATACGTGCTGCTATCCAATATGTATGCAGTGTCAGGAATGTGGGATCGTAGGGATCAGACGAGGCAATTGATGAAGGAAAGGGGTGTGAAGAAAGAGCCCGGTCGTAGCTGGATTGAGGTCAAGAATTCAGTTCATGCCTTCTTTGTCGGTGACCGACTGCACCCACTTGCAGATACGATTTATGAATTCTTAGGGGATTTGAATGAGCGAGCAGTTGAAATTGGTTATGTTGAGGATCGTTCCAACCTTTGGAGTGATATAGAACGGAAGCAGAAGGATCCAACAGTATATATTCACAGTGAGAAATTGGCAATTTCTTTTGGCCTACTCAACTTATCTAATGCTGTTCCTGTACGTGTAATGAAGAATCTTCGTGTTTGCAATGATTGCCATAATTGGATCAAGTACACGTCGAAGATTTGTAATCGAACTATCATAGTACGAGATGCTTATCGTTTTCATCATTTCAAAGATGGTGTTTGTTCTTGTAGAGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

1087

Amino Acids

121.58

Weight (kDa)

6.57

Isoelectric Point (pI)

39.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	173 - 218	2e-06	PPR repeat family
PPR_1	PF12854	374 - 403	2.2e-06	PPR repeat
PPR_2	PF13041	375 - 422	6.8e-08	PPR repeat family
PPR	PF01535	377 - 403	2.4e-07	PPR repeat
PPR_2	PF13041	476 - 522	3.2e-08	PPR repeat family
PPR_2	PF13041	577 - 624	9.2e-09	PPR repeat family
PPR	PF01535	579 - 609	1.8e-07	PPR repeat
PPR_2	PF13041	678 - 722	2.3e-08	PPR repeat family
PPR_2	PF13041	779 - 826	2.8e-09	PPR repeat family
PPR	PF01535	781 - 811	1.5e-07	PPR repeat
E_motif	PF20431	898 - 958	8.9e-20	E motif
DYW_deaminase	PF14432	994 - 1086	9e-34	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0016595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13650
fragaria_vesca	FvH4_3g25260
malus_domestica	MD12G1002600.v1.1
prunus_persica	Prupe.7G002600_v2.0.a1
pyrus_communis	pycom12g00230
rosa_chinensis	RchiOBHm_Chr5g0046881
rosa_laevigata	RLG00000034452
rosa_roxburghii	Rroxscaffold_1G00034510
rosa_rugosa	Rorug05G0232400
rosa_samantha	Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana	Rw5G029140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1137, 2222
AccB7I	CCANNNNNTGG	1 cut(s)	3006
AccBSI	CCGCTC	2 cut(s)	1071, 2413
AccII	CGCG	1 cut(s)	1265
AciI	CCGC	7 cut(s)	86, 89, 1069, 1167, 1265, 1319, 2413
AclWI	GGATC	8 cut(s)	1488, 2200, 2808, 2817, 3000, 3029, 3042, 3164
AcsI	RAATTY	7 cut(s)	278, 1098, 1400, 1704, 1902, 2881, 2942
AcuI	CTGAAG	2 cut(s)	422, 2763
AdeI	CACNNNGTG	2 cut(s)	305, 2075
AfaI	GTAC	8 cut(s)	724, 1538, 1567, 2081, 2463, 3111, 3164, 3198
AfeI	AGCGCT	2 cut(s)	149, 2094
AfiI	CCNNNNNNNGG	3 cut(s)	771, 1074, 3006
AflIII	ACRYGT	5 cut(s)	299, 424, 2139, 3112, 3165
AhlI	ACTAGT	2 cut(s)	194, 725
AjiI	CACGTC	2 cut(s)	869, 3168
AjnI	CCWGG	2 cut(s)	635, 830
AleI	CACNNNNGTG	1 cut(s)	2453
Alw21I	GWGCWC	4 cut(s)	179, 1279, 1582, 1659
Alw26I	GTCTC	5 cut(s)	23, 617, 1216, 1412, 1741
AlwI	GGATC	8 cut(s)	1488, 2200, 2808, 2817, 3000, 3029, 3042, 3164
Aor51HI	AGCGCT	2 cut(s)	149, 2094
AoxI	GGCC	3 cut(s)	439, 1000, 3079
ApoI	RAATTY	7 cut(s)	278, 1098, 1400, 1704, 1902, 2881, 2942
AseI	ATTAAT	1 cut(s)	2295
Asp700I	GAANNNNTTC	1 cut(s)	882
AspLEI	GCGC	4 cut(s)	150, 482, 1265, 2095
AspS9I	GGNCC	1 cut(s)	1432
AsuC2I	CCSGG	2 cut(s)	1759, 2856
AsuHPI	GGTGA	2 cut(s)	479, 2918
AvaII	GGWCC	1 cut(s)	1432
BaeGI	GKGCMC	1 cut(s)	507
BamHI	GGATCC	1 cut(s)	3034
BanII	GRGCYC	3 cut(s)	179, 1582, 2856
BbsI	GAAGAC	2 cut(s)	1903, 2751
Bbv12I	GWGCWC	4 cut(s)	179, 1279, 1582, 1659
BccI	CCATC	5 cut(s)	84, 431, 470, 976, 3224
BceAI	ACGGC	1 cut(s)	2589
BcgI	CGANNNNNNTGC	2 cut(s)	1652, 1686
BciT130I	CCWGG	2 cut(s)	637, 832
BciVI	GTATCC	1 cut(s)	1684
BclI	TGATCA	1 cut(s)	2358
BcnI	CCSGG	2 cut(s)	1759, 2856
BcoDI	GTCTC	5 cut(s)	23, 617, 1216, 1412, 1741
BcuI	ACTAGT	2 cut(s)	194, 725
BfmI	CTRYAG	7 cut(s)	508, 777, 1280, 1648, 1722, 2157, 2473
BfoI	RGCGCY	2 cut(s)	151, 2096
BfuI	GTATCC	1 cut(s)	1684
BglI	GCCNNNNNGGC	1 cut(s)	2484
BlpI	GCTNAGC	1 cut(s)	1674
Bme1390I	CCNGG	4 cut(s)	637, 832, 1759, 2856
Bme18I	GGWCC	1 cut(s)	1432
BmgBI	CACGTC	2 cut(s)	869, 3168
BmgT120I	GGNCC	1 cut(s)	1432
BmiI	GGNNCC	3 cut(s)	364, 2739, 3036
BmrFI	CCNGG	4 cut(s)	637, 832, 1759, 2856
BpiI	GAAGAC	2 cut(s)	1903, 2751
BpmI	CTGGAG	1 cut(s)	925
Bpu10I	CCTNAGC	3 cut(s)	1072, 1911, 2547
Bpu1102I	GCTNAGC	1 cut(s)	1674
BpuEI	CTTGAG	2 cut(s)	112, 1593
BpuMI	CCSGG	2 cut(s)	1759, 2856
Bsa29I	ATCGAT	1 cut(s)	1662
BsaAI	YACGTR	3 cut(s)	302, 2761, 3113
BsaBI	GATNNNNATC	3 cut(s)	1479, 2056, 3207
BsaI	GGTCTC	2 cut(s)	617, 1216
BsaJI	CCNNGG	3 cut(s)	291, 636, 831
BsaWI	WCCGGW	2 cut(s)	168, 359
Bsc4I	CCNNNNNNNGG	3 cut(s)	771, 1074, 3006
Bse1I	ACTGG	6 cut(s)	1627, 2233, 2368, 2633, 2739, 3260
Bse3DI	GCAATG	3 cut(s)	1051, 2655, 3145
Bse8I	GATNNNNATC	3 cut(s)	1479, 2056, 3207
BseBI	CCWGG	2 cut(s)	637, 832
BseCI	ATCGAT	1 cut(s)	1662
BseDI	CCNNGG	3 cut(s)	291, 636, 831
BseGI	GGATG	7 cut(s)	165, 613, 906, 1129, 1477, 2118, 2421
BseJI	GATNNNNATC	3 cut(s)	1479, 2056, 3207
BseLI	CCNNNNNNNGG	3 cut(s)	771, 1074, 3006
BseMI	GCAATG	3 cut(s)	1051, 2655, 3145
BseNI	ACTGG	6 cut(s)	1627, 2233, 2368, 2633, 2739, 3260
BseRI	GAGGAG	1 cut(s)	2639
BseSI	GKGCMC	1 cut(s)	507
BseYI	CCCAGC	1 cut(s)	406
BsgI	GTGCAG	2 cut(s)	943, 2900
Bsh1236I	CGCG	1 cut(s)	1265
Bsh1285I	CGRYCG	1 cut(s)	2860
BshFI	GGCC	3 cut(s)	441, 1002, 3081
BshVI	ATCGAT	1 cut(s)	1662
BsiEI	CGRYCG	1 cut(s)	2860
BsiHKAI	GWGCWC	4 cut(s)	179, 1279, 1582, 1659
BsiSI	CCGG	4 cut(s)	169, 360, 1758, 2856
BslI	CCNNNNNNNGG	3 cut(s)	771, 1074, 3006
BsmAI	GTCTC	5 cut(s)	23, 617, 1216, 1412, 1741
BsmBI	CGTCTC	1 cut(s)	1741
BsmI	GAATGC	3 cut(s)	286, 1209, 2356
BsnI	GGCC	3 cut(s)	441, 1002, 3081
Bso31I	GGTCTC	2 cut(s)	617, 1216
Bsp1286I	GDGCHC	6 cut(s)	179, 507, 1279, 1582, 1659, 2856
Bsp1720I	GCTNAGC	1 cut(s)	1674
Bsp19I	CCATGG	1 cut(s)	291
BspACI	CCGC	7 cut(s)	86, 89, 1069, 1167, 1265, 1319, 2413
BspANI	GGCC	3 cut(s)	441, 1002, 3081
BspDI	ATCGAT	1 cut(s)	1662
BspFNI	CGCG	1 cut(s)	1265
BspLI	GGNNCC	3 cut(s)	364, 2739, 3036
BspMAI	CTGCAG	3 cut(s)	1284, 1726, 2161
BspPI	GGATC	8 cut(s)	1488, 2200, 2808, 2817, 3000, 3029, 3042, 3164
BspTNI	GGTCTC	2 cut(s)	617, 1216
BsrBI	CCGCTC	2 cut(s)	1071, 2413
BsrDI	GCAATG	3 cut(s)	1051, 2655, 3145
BsrI	ACTGG	6 cut(s)	1627, 2233, 2368, 2633, 2739, 3260
BssECI	CCNNGG	3 cut(s)	291, 636, 831
BssT1I	CCWWGG	1 cut(s)	291
Bst2UI	CCWGG	2 cut(s)	637, 832
Bst4CI	ACNGT	3 cut(s)	1232, 3043, 3056
Bst6I	CTCTTC	1 cut(s)	74
BstAPI	GCANNNNNTGC	4 cut(s)	215, 1862, 2165, 3144
BstBAI	YACGTR	3 cut(s)	302, 2761, 3113
BstC8I	GCNNGC	8 cut(s)	36, 179, 947, 958, 1261, 2566, 2677, 2967
BstDSI	CCRYGG	1 cut(s)	291
BstEII	GGTNACC	1 cut(s)	2906
BstF5I	GGATG	7 cut(s)	165, 613, 906, 1129, 1477, 2118, 2421
BstFNI	CGCG	1 cut(s)	1265
BstH2I	RGCGCY	2 cut(s)	151, 2096
BstHHI	GCGC	4 cut(s)	150, 482, 1265, 2095
BstMAI	GTCTC	5 cut(s)	23, 617, 1216, 1412, 1741
BstMCI	CGRYCG	1 cut(s)	2860
BstNI	CCWGG	2 cut(s)	637, 832
BstNSI	RCATGY	6 cut(s)	428, 654, 1263, 1514, 1869, 2143
BstPI	GGTNACC	1 cut(s)	2906
BstSCI	CCNGG	4 cut(s)	635, 830, 1757, 2854
BstSFI	CTRYAG	7 cut(s)	508, 777, 1280, 1648, 1722, 2157, 2473
BstSLI	GKGCMC	1 cut(s)	507
BstUI	CGCG	1 cut(s)	1265
BstV2I	GAAGAC	2 cut(s)	1903, 2751
BstX2I	RGATCY	2 cut(s)	2205, 3034
BstYI	RGATCY	2 cut(s)	2205, 3034
Bsu15I	ATCGAT	1 cut(s)	1662
BsuI	GTATCC	1 cut(s)	1684
BsuRI	GGCC	3 cut(s)	441, 1002, 3081
BsuTUI	ATCGAT	1 cut(s)	1662
BtgI	CCRYGG	1 cut(s)	291
BtgZI	GCGATG	2 cut(s)	62, 450
BtrI	CACGTC	2 cut(s)	869, 3168
BtsCI	GGATG	7 cut(s)	165, 613, 906, 1129, 1477, 2118, 2421
BtsI	GCAGTG	2 cut(s)	1719, 2790
BtsIMutI	CAGTG	8 cut(s)	1142, 1169, 1719, 2361, 2640, 2694, 2790, 3061
Cac8I	GCNNGC	8 cut(s)	36, 179, 947, 958, 1261, 2566, 2677, 2967
CfoI	GCGC	4 cut(s)	150, 482, 1265, 2095
Cfr13I	GGNCC	1 cut(s)	1432
ClaI	ATCGAT	1 cut(s)	1662
Csp6I	GTAC	8 cut(s)	723, 1537, 1566, 2080, 2462, 3110, 3163, 3197
CviQI	GTAC	8 cut(s)	723, 1537, 1566, 2080, 2462, 3110, 3163, 3197
DraI	TTTAAA	1 cut(s)	342
DraIII	CACNNNGTG	2 cut(s)	305, 2075
Eam1104I	CTCTTC	1 cut(s)	74
EarI	CTCTTC	1 cut(s)	74
Ecl136II	GAGCTC	2 cut(s)	177, 1580
Eco130I	CCWWGG	1 cut(s)	291
Eco24I	GRGCYC	3 cut(s)	179, 1582, 2856
Eco31I	GGTCTC	2 cut(s)	617, 1216
Eco32I	GATATC	1 cut(s)	2058
Eco47I	GGWCC	1 cut(s)	1432
Eco47III	AGCGCT	2 cut(s)	149, 2094
Eco53kI	GAGCTC	2 cut(s)	177, 1580
Eco57I	CTGAAG	2 cut(s)	422, 2763
Eco91I	GGTNACC	1 cut(s)	2906
EcoICRI	GAGCTC	2 cut(s)	177, 1580
EcoO65I	GGTNACC	1 cut(s)	2906
EcoRI	GAATTC	4 cut(s)	278, 1704, 2881, 2942
EcoRII	CCWGG	2 cut(s)	635, 830
EcoRV	GATATC	1 cut(s)	2058
EcoT14I	CCWWGG	1 cut(s)	291
EcoT22I	ATGCAT	1 cut(s)	1512
EcoT38I	GRGCYC	3 cut(s)	179, 1582, 2856
ErhI	CCWWGG	1 cut(s)	291
Esp3I	CGTCTC	1 cut(s)	1741
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FauI	CCCGC	3 cut(s)	93, 1076, 1312
FauNDI	CATATG	1 cut(s)	1036
FbaI	TGATCA	1 cut(s)	2358
FokI	GGATG	7 cut(s)	152, 600, 913, 1136, 1484, 2125, 2428
FriOI	GRGCYC	3 cut(s)	179, 1582, 2856
GlaI	GCGC	4 cut(s)	149, 481, 1264, 2094
GsaI	CCCAGC	1 cut(s)	410
GsuI	CTGGAG	1 cut(s)	925
HaeII	RGCGCY	2 cut(s)	151, 2096
HaeIII	GGCC	3 cut(s)	441, 1002, 3081
HapII	CCGG	4 cut(s)	169, 360, 1758, 2856
HhaI	GCGC	4 cut(s)	150, 482, 1265, 2095
Hin6I	GCGC	4 cut(s)	148, 480, 1263, 2093
HinP1I	GCGC	4 cut(s)	148, 480, 1263, 2093
HincII	GTYRAC	1 cut(s)	564
HindII	GTYRAC	1 cut(s)	564
HindIII	AAGCTT	1 cut(s)	383
HpaII	CCGG	4 cut(s)	169, 360, 1758, 2856
HphI	GGTGA	2 cut(s)	479, 2918
Hpy166II	GTNNAC	4 cut(s)	564, 1539, 2145, 3165
Hpy188III	TCNNGA	7 cut(s)	591, 617, 1478, 1977, 2729, 2790, 2878
Hpy8I	GTNNAC	4 cut(s)	564, 1539, 2145, 3165
Hpy99I	CGWCG	2 cut(s)	233, 3172
HpyAV	CCTTC	7 cut(s)	248, 1279, 1340, 2492, 2824, 2905, 3025
HpyCH4III	ACNGT	3 cut(s)	1232, 3043, 3056
HpyCH4IV	ACGT	8 cut(s)	301, 629, 868, 908, 1058, 2760, 3112, 3167
HpySE526I	ACGT	8 cut(s)	301, 629, 868, 908, 1058, 2760, 3112, 3167
HspAI	GCGC	4 cut(s)	148, 480, 1263, 2093
Ksp22I	TGATCA	1 cut(s)	2358
LmnI	GCTCC	3 cut(s)	356, 1577, 1684
MaeII	ACGT	8 cut(s)	301, 629, 868, 908, 1058, 2760, 3112, 3167
MaeIII	GTNAC	5 cut(s)	1054, 1234, 1963, 2446, 2906
MbiI	CCGCTC	2 cut(s)	1071, 2413
MfeI	CAATTG	2 cut(s)	1239, 2822
MflI	RGATCY	2 cut(s)	2205, 3034
MhlI	GDGCHC	6 cut(s)	179, 507, 1279, 1582, 1659, 2856
MlyI	GAGTC	4 cut(s)	37, 1710, 2656, 2741
MmeI	TCCRAC	5 cut(s)	1449, 1555, 1930, 3024, 3062
Mph1103I	ATGCAT	1 cut(s)	1512
MroXI	GAANNNNTTC	1 cut(s)	882
MseI	TTAA	7 cut(s)	341, 644, 978, 1635, 2264, 2295, 2669
MslI	CAYNNNNRTG	8 cut(s)	249, 618, 899, 1039, 1635, 1664, 2382, 2453
MspI	CCGG	4 cut(s)	169, 360, 1758, 2856
MspR9I	CCNGG	4 cut(s)	637, 832, 1759, 2856
MunI	CAATTG	2 cut(s)	1239, 2822
Mva1269I	GAATGC	3 cut(s)	286, 1209, 2356
MvaI	CCWGG	2 cut(s)	637, 832
MvnI	CGCG	1 cut(s)	1265
NciI	CCSGG	2 cut(s)	1759, 2856
NcoI	CCATGG	1 cut(s)	291
NdeI	CATATG	1 cut(s)	1036
NlaIV	GGNNCC	3 cut(s)	364, 2739, 3036
NmuCI	GTSAC	3 cut(s)	1054, 2446, 2906
NsiI	ATGCAT	1 cut(s)	1512
NspI	RCATGY	6 cut(s)	428, 654, 1263, 1514, 1869, 2143
OliI	CACNNNNGTG	1 cut(s)	2453
PaeI	GCATGC	1 cut(s)	1263
PciI	ACATGT	2 cut(s)	424, 2139
PcsI	WCGNNNNNNNCGW	1 cut(s)	307
PctI	GAATGC	3 cut(s)	286, 1209, 2356
PdmI	GAANNNNTTC	1 cut(s)	882
PflFI	GACNNNGTC	1 cut(s)	1733
PflMI	CCANNNNNTGG	1 cut(s)	3006
PleI	GAGTC	4 cut(s)	37, 1710, 2656, 2741
PpsI	GAGTC	4 cut(s)	37, 1710, 2656, 2741
Ppu21I	YACGTR	3 cut(s)	302, 2761, 3113
PscI	ACATGT	2 cut(s)	424, 2139
PshBI	ATTAAT	1 cut(s)	2295
PsiI	TTATAA	2 cut(s)	1137, 2222
Psp124BI	GAGCTC	2 cut(s)	179, 1582
Psp6I	CCWGG	2 cut(s)	635, 830
PspEI	GGTNACC	1 cut(s)	2906
PspFI	CCCAGC	1 cut(s)	406
PspGI	CCWGG	2 cut(s)	635, 830
PspN4I	GGNNCC	3 cut(s)	364, 2739, 3036
PspPI	GGNCC	1 cut(s)	1432
PsrI	GAACNNNNNNTAC	2 cut(s)	2578, 2610
PstI	CTGCAG	3 cut(s)	1284, 1726, 2161
PsuI	RGATCY	2 cut(s)	2205, 3034
PsyI	GACNNNGTC	1 cut(s)	1733
RsaI	GTAC	8 cut(s)	724, 1538, 1567, 2081, 2463, 3111, 3164, 3198
RsaNI	GTAC	8 cut(s)	723, 1537, 1566, 2080, 2462, 3110, 3163, 3197
RseI	CAYNNNNRTG	8 cut(s)	249, 618, 899, 1039, 1635, 1664, 2382, 2453
SacI	GAGCTC	2 cut(s)	179, 1582
SaqAI	TTAA	7 cut(s)	341, 644, 978, 1635, 2264, 2295, 2669
Sau96I	GGNCC	1 cut(s)	1432
SchI	GAGTC	4 cut(s)	37, 1710, 2656, 2741
ScrFI	CCNGG	4 cut(s)	637, 832, 1759, 2856
SduI	GDGCHC	6 cut(s)	179, 507, 1279, 1582, 1659, 2856
SfcI	CTRYAG	7 cut(s)	508, 777, 1280, 1648, 1722, 2157, 2473
SinI	GGWCC	1 cut(s)	1432
SmiMI	CAYNNNNRTG	8 cut(s)	249, 618, 899, 1039, 1635, 1664, 2382, 2453
SmlI	CTYRAG	2 cut(s)	127, 1608
SmoI	CTYRAG	2 cut(s)	127, 1608
SpeI	ACTAGT	2 cut(s)	194, 725
SphI	GCATGC	1 cut(s)	1263
SsiI	CCGC	7 cut(s)	86, 89, 1069, 1167, 1265, 1319, 2413
SstI	GAGCTC	2 cut(s)	179, 1582
StyD4I	CCNGG	4 cut(s)	635, 830, 1757, 2854
StyI	CCWWGG	1 cut(s)	291
TaaI	ACNGT	3 cut(s)	1232, 3043, 3056
TaiI	ACGT	8 cut(s)	304, 632, 871, 911, 1061, 2763, 3115, 3170
TaqI	TCGA	4 cut(s)	228, 1662, 3170, 3184
TaqII	GACCGA	1 cut(s)	2925
TatI	WGTACW	2 cut(s)	1536, 3162
TauI	GCSGC	1 cut(s)	91
Tru1I	TTAA	7 cut(s)	341, 644, 978, 1635, 2264, 2295, 2669
Tru9I	TTAA	7 cut(s)	341, 644, 978, 1635, 2264, 2295, 2669
TscAI	CASTG	8 cut(s)	1149, 1169, 1726, 2368, 2640, 2701, 2790, 3061
TseFI	GTSAC	3 cut(s)	1054, 2446, 2906
Tsp45I	GTSAC	3 cut(s)	1054, 2446, 2906
TspGWI	ACGGA	1 cut(s)	3037
TspRI	CASTG	8 cut(s)	1149, 1169, 1726, 2368, 2640, 2701, 2790, 3061
Tth111I	GACNNNGTC	1 cut(s)	1733
Van91I	CCANNNNNTGG	1 cut(s)	3006
VpaK11BI	GGWCC	1 cut(s)	1432
VspI	ATTAAT	1 cut(s)	2295
XapI	RAATTY	7 cut(s)	278, 1098, 1400, 1704, 1902, 2881, 2942
XbaI	TCTAGA	2 cut(s)	1976, 2728
XceI	RCATGY	6 cut(s)	428, 654, 1263, 1514, 1869, 2143
XmnI	GAANNNNTTC	1 cut(s)	882
Zsp2I	ATGCAT	1 cut(s)	1512

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.