Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0016595

Physical Location & Seq

Genomic Coordinates Reverse (-)

434228 .. 438771

Gene Structure 4544 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1002600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3255 bp

ATGAGTGGCTCTGGCTGTCTCAAATCTTTGATGCTCGCTCTGACTCTCCACCAATTCCCCGACCCCCTCTTCTTCCATCGCTTCCCGCCGCTTCATTTCACAACCAAACCCAAACCCACCTCAAGTTTCAATCTTCACAAGCGCTTTCGTGGGAGTGTCATCCGGTTGAGCTCGCCAGAAGAATATACTAGTAATGCAGCAGCAGCCAATGCCCATGTCCTCGACGACTCTCTACTTCATCAAAATGAAGGTAATAATCCAAAGGGGATTGAATTCCTGCATTCCATGGAAACACGTAGTGTTCGTGCTAATTGTCAGACATATATTTGGCTTTTAAAAGGCTGCTCCAATTCCGGTTCCTTGTTAGATTCTAAAAAGCTTCACTCTAGGATTCTGAAGCTGGGTTTTGATGCTGAACATGTAATATGCGATGGCCTTATTGATGCTTACCTTGCCGCTGGTGATTTAGATTGCGCAGTTAGGGTTTTTGATGATGTGCCCTACAGAAGTTTGTTTTCATGGAATAACATAATTCAAGTGTTTCTTGCAAACAAGTTGACCAGTCAAGTATTGGATTTCTTTTCCCGAATGTTAGCAGATAATGTGCATCCTGATGAGACCACGTTTTCCAGGGTTTTAAGGGCATGTGGTGGCAGTAATGTTCATCTTCAATATGTCAAACAGATTCACGCTAGGGTTATCTGTCATGGTTTTGGTACTAGTTTGCTTGTGTGTAATCCCTTGATTGATTTGTATGCCAAAAACGGGTCTGTAGATGCTGCTAAAAAGGTCTTTGATAAATTGTATATAAGGGATAGCGTTTCCTGGGTTGCCATGATTTCTGGTTTATCTCAGAATGGACGCGAAAAAGAAGCAATTCTCTTATTCATTGAGATGCAAACGTCAGATATTTTACCCACTCCTTATGTGTTCTCCAGCGTGCTAAGTGCCTGCGCCAAAATAGAGTTATTTAAGATGGGAGAGCAACTGCATGGACTTATTTTCAAGGGGGGTTTTTCTTGTGAAACATATGTATGCAATGCACTTGTGACGTTATATTCCCGCTCAGGGAACTTTATATCTGCTGAACAAATTTTCAAAACAATGTGGCACAGGGATGCAGTATCTTATAACTCACTGATCTCTGGGTTAGCTCAGTGCGGATTTAGTGATAGAGCATTGGAATTGTTCAAAAGAATGCAGATTGATTGTTTGAGACCAGACTGTGTTACAATTGCTAGTCTTTTGAGCGCATGCGCCGAAATTGGTGCTCTGCAGAAGGGAAAACAACTCCACTCACTTGCAATAAAAGCGGGAATGTCATCAGATATTATCCTTGAAGGTTCTCTGCTTGATCTTTATGTGAAATGCTCAGATGTGCAAACTGCCTATGATTTTTTCCTTACAACAGAGACAGAAAATGTGGTCCTGTGGAATGTAATGCTAGTTGCTTATGGTCAGTTGGATGATCTGGATCAATCATTTCGGATATTTAGGCAAATGCATGTTGAGGGCATGATTCCTAATCAGTACACCTACCCTAGTATCCTAAGAACCTGTACCTCTGTTGGAGCTCTCAATTTGGGAGAGCAGATACATACTCAAGTCATAAAAACTGGTTTTCATTTTAATGTTTATGTCTGTAGTGTGCTCATTGATATGTATGCTAAGCATGGGGAGCTGGATACCGCCCTGAGAATTCTCAGACGACTCACTGCAGATGACGTCGTCTCATGGACAGCTATGATAGCCGGGTATGCACAGCATGATCTATTTTCTGAATCTCTGATACTTTTTGAAGAAATGCAAAGGCGAGGGATTCAGTCTGACAACATAGGATTTTCAAGTGCAATAAGTGCATGTGCTGGTATTCAAGCACTTCGTCAAGGAAGACAAATTCATGCTCAGTCGTGTGTCTTTGGTTATTCAGATGATCTTTCAGTTGGAAATGCACTTGTTACCCTTTACGCTAGATGTGGTAGAATACAGGAAGCGTACCGAGCATTTGAGGCAACTGATTCTAAAGATAATATGTCATGGAATGGATTGATATCTGGGTTTGCACAGAGTGGGAACTATGAGGAAGCACTGCAGGTATTTACTCGGATGAATAAAGCTGGAATAGAAGCTAACATGTTCACATTTGGCTCTGCAGTTAGCGCTGCTGCTAACTTAACAAATATAAAACAAGGGCAGCAGATTCATGCCACAATTATAAAAACTGGCAGCAATTCCGAAACGGAGGTTTCCAATGCGTTAATCACATTGTATTCAAAGTGTGGTAGCATTGATGATGCCAAGAGAGAGTTTTCTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAACATGGGCGTGGTATTGAATCAATACACCTATTTGAGCAGATGAAACAACTTGGTATAGCACCAAGCCATGTCACATTTGTGGGGGTACTTACAGCCTGTAGCCATATAGGCTTGGTAAATGAAGGGCTTGGCTACTTTGAATCCATGAGAAAAGAGCATGGGTTAGTGCCTAAGCCTGAACACTATGCTTGCGTTGTTGATCTTCTAGGTAGAGCTGGTTCTCTGAGCCGTGCCAGAAAGTTTATAGAGGAGATGCCAGTGAAACCAGATGCAATGATTTGGAGGACTCTTTTAAGTGCTTGCATAACTCGCAAGAACACTGAAATTGGAGAGTTTTCTGCCAACCATCTTCTAGAACTGGAACCTGAAGACTCAGCAACGTACGTGCTGCTATCCAATATGTACGCAGTGTCAGGAATGTGGGATCGTAGGGATCAGACGAGGCAATTGATGAAGGAAAGGGGTGTGAAGAAAGAGCCCGGTCGTAGCTGGATTGAGGTCAAGAATTCAGTTCATGCCTTCTTTGTCGGTGACCGACTGCACCCACTTGCAGATACGATTTATGAATTCTTAGGGGATTTGAATGAGCGGGCAGCTGAAATTGGTTATGTTGAGGATCGTTCCAACCTTTGGAATGATATGGAACAGAAGCAGAAGGATCCAACAGTATATATCCACAGTGAGAAATTGGCAATTTCTTTTGGCCTACTCAACTTATCTAATGCTGTTCCTATACGTGTAATGAAGAATCTTCGTGTTTGCAATGATTGCCATAATTGGATCAAGTACACGTCGAAGATTTGTAATCGAACTATCATAGTACGAGATGCTTATCGTTTTCATCATTTCAAAGATGGTGCTTGTTCTTGTAGAGATTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

1085

Amino Acids

121.4

Weight (kDa)

6.54

Isoelectric Point (pI)

40.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	171 - 216	1.1e-06	PPR repeat family
PPR_2	PF13041	272 - 320	2.4e-07	PPR repeat family
PPR_1	PF12854	372 - 401	2.1e-06	PPR repeat
PPR_2	PF13041	373 - 420	6.8e-08	PPR repeat family
PPR	PF01535	375 - 401	2.4e-07	PPR repeat
PPR_2	PF13041	474 - 520	3.2e-08	PPR repeat family
PPR_2	PF13041	575 - 622	5e-09	PPR repeat family
PPR	PF01535	577 - 607	1.8e-07	PPR repeat
PPR_2	PF13041	676 - 720	1.4e-08	PPR repeat family
PPR	PF01535	679 - 708	6.2e-07	PPR repeat
PPR_2	PF13041	777 - 824	9.5e-10	PPR repeat family
PPR	PF01535	779 - 809	2.1e-07	PPR repeat
E_motif	PF20431	896 - 956	8.7e-19	E motif
DYW_deaminase	PF14432	992 - 1084	2.7e-34	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016595)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13650
fragaria_vesca	FvH4_3g25260
malus_domestica	MD12G1002600.v1.1
prunus_persica	Prupe.7G002600_v2.0.a1
pyrus_communis	pycom12g00230
rosa_chinensis	RchiOBHm_Chr5g0046881
rosa_laevigata	RLG00000034452
rosa_roxburghii	Rroxscaffold_1G00034510
rosa_rugosa	Rorug05G0232400
rosa_samantha	Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana	Rw5G029140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1131, 2216
AatII	GACGTC	1 cut(s)	1728
Acc16I	TGCGCA	1 cut(s)	475
Acc36I	ACCTGC	1 cut(s)	2083
AccB7I	CCANNNNNTGG	1 cut(s)	3000
AccBSI	CCGCTC	2 cut(s)	1065, 2959
AccII	CGCG	1 cut(s)	864
AciI	CCGC	8 cut(s)	86, 89, 456, 1063, 1161, 1313, 1689, 2959
AclWI	GGATC	7 cut(s)	1482, 2802, 2811, 2994, 3023, 3036, 3158
AcsI	RAATTY	6 cut(s)	272, 1092, 1698, 1896, 2875, 2936
AcuI	CTGAAG	2 cut(s)	416, 2757
AcyI	GRCGYC	1 cut(s)	1725
AdeI	CACNNNGTG	2 cut(s)	299, 2069
AfaI	GTAC	9 cut(s)	718, 1532, 1561, 1997, 2457, 2753, 2774, 3158, 3192
AfeI	AGCGCT	2 cut(s)	143, 2161
AfiI	CCNNNNNNNGG	3 cut(s)	765, 1068, 3000
AflIII	ACRYGT	5 cut(s)	293, 418, 2133, 3106, 3159
AhlI	ACTAGT	2 cut(s)	188, 719
AjiI	CACGTC	1 cut(s)	3162
AjnI	CCWGG	2 cut(s)	629, 824
AleI	CACNNNNGTG	1 cut(s)	2447
Alw21I	GWGCWC	4 cut(s)	173, 1273, 1576, 1653
Alw26I	GTCTC	5 cut(s)	23, 611, 1210, 1406, 1735
AlwI	GGATC	7 cut(s)	1482, 2802, 2811, 2994, 3023, 3036, 3158
Aor51HI	AGCGCT	2 cut(s)	143, 2161
AoxI	GGCC	2 cut(s)	433, 3073
ApoI	RAATTY	6 cut(s)	272, 1092, 1698, 1896, 2875, 2936
Asp700I	GAANNNNTTC	1 cut(s)	876
AspLEI	GCGC	6 cut(s)	144, 476, 956, 1253, 1259, 2162
AspS9I	GGNCC	1 cut(s)	1426
AsuC2I	CCSGG	2 cut(s)	1753, 2850
AsuHPI	GGTGA	2 cut(s)	473, 2912
AvaII	GGWCC	1 cut(s)	1426
BaeGI	GKGCMC	1 cut(s)	501
BaeI	ACNNNNGTAYC	2 cut(s)	1528, 1561
BamHI	GGATCC	1 cut(s)	3028
BanII	GRGCYC	3 cut(s)	173, 1576, 2850
BbsI	GAAGAC	2 cut(s)	1897, 2745
Bbv12I	GWGCWC	4 cut(s)	173, 1273, 1576, 1653
BccI	CCATC	5 cut(s)	84, 425, 970, 2724, 3218
BceAI	ACGGC	1 cut(s)	2583
BcgI	CGANNNNNNTGC	2 cut(s)	1250, 1284
BciT130I	CCWGG	2 cut(s)	631, 826
BciVI	GTATCC	2 cut(s)	1556, 1678
BclI	TGATCA	1 cut(s)	2352
BcnI	CCSGG	2 cut(s)	1753, 2850
BcoDI	GTCTC	5 cut(s)	23, 611, 1210, 1406, 1735
BcuI	ACTAGT	2 cut(s)	188, 719
BfmI	CTRYAG	8 cut(s)	502, 771, 1274, 1642, 1716, 2090, 2151, 2467
BfoI	RGCGCY	2 cut(s)	145, 2163
BfuAI	ACCTGC	1 cut(s)	2083
BfuI	GTATCC	2 cut(s)	1556, 1678
BglI	GCCNNNNNGGC	1 cut(s)	2478
BlpI	GCTNAGC	1 cut(s)	1668
Bme1390I	CCNGG	4 cut(s)	631, 826, 1753, 2850
Bme18I	GGWCC	1 cut(s)	1426
BmgBI	CACGTC	1 cut(s)	3162
BmgT120I	GGNCC	1 cut(s)	1426
BmiI	GGNNCC	3 cut(s)	358, 2733, 3030
BmrFI	CCNGG	4 cut(s)	631, 826, 1753, 2850
BpiI	GAAGAC	2 cut(s)	1897, 2745
BpmI	CTGGAG	1 cut(s)	919
Bpu10I	CCTNAGC	2 cut(s)	1066, 2541
Bpu1102I	GCTNAGC	1 cut(s)	1668
BpuEI	CTTGAG	2 cut(s)	106, 1587
BpuMI	CCSGG	2 cut(s)	1753, 2850
BsaAI	YACGTR	3 cut(s)	296, 2755, 3107
BsaBI	GATNNNNATC	3 cut(s)	1473, 2050, 3201
BsaHI	GRCGYC	1 cut(s)	1725
BsaI	GGTCTC	2 cut(s)	611, 1210
BsaJI	CCNNGG	3 cut(s)	285, 630, 825
BsaWI	WCCGGW	2 cut(s)	162, 353
Bsc4I	CCNNNNNNNGG	3 cut(s)	765, 1068, 3000
Bse1I	ACTGG	7 cut(s)	561, 1621, 2227, 2362, 2627, 2733, 3254
Bse3DI	GCAATG	3 cut(s)	1045, 2649, 3139
Bse8I	GATNNNNATC	3 cut(s)	1473, 2050, 3201
BseBI	CCWGG	2 cut(s)	631, 826
BseDI	CCNNGG	3 cut(s)	285, 630, 825
BseGI	GGATG	5 cut(s)	159, 607, 1123, 1471, 2112
BseJI	GATNNNNATC	3 cut(s)	1473, 2050, 3201
BseLI	CCNNNNNNNGG	3 cut(s)	765, 1068, 3000
BseMI	GCAATG	3 cut(s)	1045, 2649, 3139
BseMII	CTCAG	9 cut(s)	866, 1080, 1169, 1386, 1685, 1717, 1919, 2585, 2757
BseNI	ACTGG	7 cut(s)	561, 1621, 2227, 2362, 2627, 2733, 3254
BseRI	GAGGAG	1 cut(s)	2633
BseSI	GKGCMC	1 cut(s)	501
BseYI	CCCAGC	1 cut(s)	400
BsgI	GTGCAG	1 cut(s)	2894
Bsh1236I	CGCG	1 cut(s)	864
Bsh1285I	CGRYCG	1 cut(s)	2854
BshFI	GGCC	2 cut(s)	435, 3075
BsiEI	CGRYCG	1 cut(s)	2854
BsiHKAI	GWGCWC	4 cut(s)	173, 1273, 1576, 1653
BsiSI	CCGG	4 cut(s)	163, 354, 1752, 2850
BsiWI	CGTACG	1 cut(s)	2751
BslI	CCNNNNNNNGG	3 cut(s)	765, 1068, 3000
BsmAI	GTCTC	5 cut(s)	23, 611, 1210, 1406, 1735
BsmBI	CGTCTC	1 cut(s)	1735
BsmI	GAATGC	3 cut(s)	280, 1203, 2350
BsnI	GGCC	2 cut(s)	435, 3075
Bso31I	GGTCTC	2 cut(s)	611, 1210
Bsp1286I	GDGCHC	6 cut(s)	173, 501, 1273, 1576, 1653, 2850
Bsp1720I	GCTNAGC	1 cut(s)	1668
Bsp19I	CCATGG	1 cut(s)	285
BspACI	CCGC	8 cut(s)	86, 89, 456, 1063, 1161, 1313, 1689, 2959
BspANI	GGCC	2 cut(s)	435, 3075
BspCNI	CTCAG	9 cut(s)	865, 1079, 1168, 1385, 1686, 1716, 1918, 2586, 2756
BspFNI	CGCG	1 cut(s)	864
BspLI	GGNNCC	3 cut(s)	358, 2733, 3030
BspMAI	CTGCAG	4 cut(s)	1278, 1720, 2094, 2155
BspMI	ACCTGC	1 cut(s)	2083
BspPI	GGATC	7 cut(s)	1482, 2802, 2811, 2994, 3023, 3036, 3158
BspTNI	GGTCTC	2 cut(s)	611, 1210
BsrBI	CCGCTC	2 cut(s)	1065, 2959
BsrDI	GCAATG	3 cut(s)	1045, 2649, 3139
BsrI	ACTGG	7 cut(s)	561, 1621, 2227, 2362, 2627, 2733, 3254
BssECI	CCNNGG	3 cut(s)	285, 630, 825
BssNI	GRCGYC	1 cut(s)	1725
BssT1I	CCWWGG	1 cut(s)	285
Bst2UI	CCWGG	2 cut(s)	631, 826
Bst4CI	ACNGT	3 cut(s)	1226, 3037, 3050
Bst6I	CTCTTC	1 cut(s)	74
BstACI	GRCGYC	1 cut(s)	1725
BstAPI	GCANNNNNTGC	3 cut(s)	209, 1856, 3138
BstBAI	YACGTR	3 cut(s)	296, 2755, 3107
BstC8I	GCNNGC	8 cut(s)	36, 173, 941, 952, 1255, 2560, 2671, 2961
BstDSI	CCRYGG	1 cut(s)	285
BstEII	GGTNACC	1 cut(s)	2900
BstF5I	GGATG	5 cut(s)	159, 607, 1123, 1471, 2112
BstFNI	CGCG	1 cut(s)	864
BstH2I	RGCGCY	2 cut(s)	145, 2163
BstHHI	GCGC	6 cut(s)	144, 476, 956, 1253, 1259, 2162
BstMAI	GTCTC	5 cut(s)	23, 611, 1210, 1406, 1735
BstMCI	CGRYCG	1 cut(s)	2854
BstNI	CCWGG	2 cut(s)	631, 826
BstNSI	RCATGY	6 cut(s)	422, 648, 1257, 1508, 1863, 2137
BstPI	GGTNACC	1 cut(s)	2900
BstSCI	CCNGG	4 cut(s)	629, 824, 1751, 2848
BstSFI	CTRYAG	8 cut(s)	502, 771, 1274, 1642, 1716, 2090, 2151, 2467
BstSLI	GKGCMC	1 cut(s)	501
BstUI	CGCG	1 cut(s)	864
BstV2I	GAAGAC	2 cut(s)	1897, 2745
BstX2I	RGATCY	1 cut(s)	3028
BstYI	RGATCY	1 cut(s)	3028
BsuI	GTATCC	2 cut(s)	1556, 1678
BsuRI	GGCC	2 cut(s)	435, 3075
BtgI	CCRYGG	1 cut(s)	285
BtgZI	GCGATG	2 cut(s)	62, 444
BtrI	CACGTC	1 cut(s)	3162
BtsCI	GGATG	5 cut(s)	159, 607, 1123, 1471, 2112
BtsI	GCAGTG	3 cut(s)	1713, 2087, 2784
BtsIMutI	CAGTG	9 cut(s)	1136, 1163, 1713, 2087, 2355, 2634, 2688, 2784, 3055
BveI	ACCTGC	1 cut(s)	2083
Cac8I	GCNNGC	8 cut(s)	36, 173, 941, 952, 1255, 2560, 2671, 2961
CfoI	GCGC	6 cut(s)	144, 476, 956, 1253, 1259, 2162
Cfr13I	GGNCC	1 cut(s)	1426
CseI	GACGC	1 cut(s)	870
Csp6I	GTAC	9 cut(s)	717, 1531, 1560, 1996, 2456, 2752, 2773, 3157, 3191
CviQI	GTAC	9 cut(s)	717, 1531, 1560, 1996, 2456, 2752, 2773, 3157, 3191
DraI	TTTAAA	1 cut(s)	336
DraIII	CACNNNGTG	2 cut(s)	299, 2069
Eam1104I	CTCTTC	1 cut(s)	74
EarI	CTCTTC	1 cut(s)	74
Ecl136II	GAGCTC	2 cut(s)	171, 1574
Eco130I	CCWWGG	1 cut(s)	285
Eco24I	GRGCYC	3 cut(s)	173, 1576, 2850
Eco31I	GGTCTC	2 cut(s)	611, 1210
Eco32I	GATATC	1 cut(s)	2052
Eco47I	GGWCC	1 cut(s)	1426
Eco47III	AGCGCT	2 cut(s)	143, 2161
Eco53kI	GAGCTC	2 cut(s)	171, 1574
Eco57I	CTGAAG	2 cut(s)	416, 2757
Eco91I	GGTNACC	1 cut(s)	2900
EcoICRI	GAGCTC	2 cut(s)	171, 1574
EcoO65I	GGTNACC	1 cut(s)	2900
EcoRI	GAATTC	4 cut(s)	272, 1698, 2875, 2936
EcoRII	CCWGG	2 cut(s)	629, 824
EcoRV	GATATC	1 cut(s)	2052
EcoT14I	CCWWGG	1 cut(s)	285
EcoT22I	ATGCAT	1 cut(s)	1506
EcoT38I	GRGCYC	3 cut(s)	173, 1576, 2850
ErhI	CCWWGG	1 cut(s)	285
Esp3I	CGTCTC	1 cut(s)	1735
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FauI	CCCGC	4 cut(s)	93, 1070, 1306, 2952
FauNDI	CATATG	1 cut(s)	1030
FbaI	TGATCA	1 cut(s)	2352
FokI	GGATG	5 cut(s)	146, 594, 1130, 1478, 2119
FriOI	GRGCYC	3 cut(s)	173, 1576, 2850
FspI	TGCGCA	1 cut(s)	475
GlaI	GCGC	6 cut(s)	143, 475, 955, 1252, 1258, 2161
GsaI	CCCAGC	1 cut(s)	404
GsuI	CTGGAG	1 cut(s)	919
HaeII	RGCGCY	2 cut(s)	145, 2163
HaeIII	GGCC	2 cut(s)	435, 3075
HapII	CCGG	4 cut(s)	163, 354, 1752, 2850
HgaI	GACGC	1 cut(s)	870
HhaI	GCGC	6 cut(s)	144, 476, 956, 1253, 1259, 2162
Hin1I	GRCGYC	1 cut(s)	1725
Hin6I	GCGC	6 cut(s)	142, 474, 954, 1251, 1257, 2160
HinP1I	GCGC	6 cut(s)	142, 474, 954, 1251, 1257, 2160
HincII	GTYRAC	1 cut(s)	558
HindII	GTYRAC	1 cut(s)	558
HindIII	AAGCTT	1 cut(s)	377
HpaII	CCGG	4 cut(s)	163, 354, 1752, 2850
HphI	GGTGA	2 cut(s)	473, 2912
Hpy166II	GTNNAC	4 cut(s)	558, 1533, 2139, 3159
Hpy188III	TCNNGA	6 cut(s)	585, 611, 1472, 2723, 2784, 2872
Hpy8I	GTNNAC	4 cut(s)	558, 1533, 2139, 3159
Hpy99I	CGWCG	3 cut(s)	227, 1730, 3166
HpyAV	CCTTC	7 cut(s)	242, 1273, 1334, 2486, 2818, 2899, 3019
HpyCH4III	ACNGT	3 cut(s)	1226, 3037, 3050
HpyCH4IV	ACGT	9 cut(s)	295, 623, 902, 1052, 1725, 2750, 2754, 3106, 3161
HpySE526I	ACGT	9 cut(s)	295, 623, 902, 1052, 1725, 2750, 2754, 3106, 3161
Hsp92I	GRCGYC	1 cut(s)	1725
HspAI	GCGC	6 cut(s)	142, 474, 954, 1251, 1257, 2160
Ksp22I	TGATCA	1 cut(s)	2352
LmnI	GCTCC	3 cut(s)	350, 1571, 1678
MaeII	ACGT	9 cut(s)	295, 623, 902, 1052, 1725, 2750, 2754, 3106, 3161
MaeIII	GTNAC	5 cut(s)	1048, 1228, 1957, 2440, 2900
MbiI	CCGCTC	2 cut(s)	1065, 2959
MfeI	CAATTG	2 cut(s)	1233, 2816
MflI	RGATCY	1 cut(s)	3028
MhlI	GDGCHC	6 cut(s)	173, 501, 1273, 1576, 1653, 2850
MlyI	GAGTC	5 cut(s)	37, 221, 1704, 2650, 2735
MmeI	TCCRAC	5 cut(s)	1443, 1549, 1924, 3018, 3056
Mph1103I	ATGCAT	1 cut(s)	1506
MroXI	GAANNNNTTC	1 cut(s)	876
MseI	TTAA	7 cut(s)	335, 638, 972, 1629, 2174, 2258, 2663
MslI	CAYNNNNRTG	9 cut(s)	243, 612, 893, 1033, 1116, 1629, 1658, 2376, 2447
MspA1I	CMGCKG	2 cut(s)	458, 2966
MspI	CCGG	4 cut(s)	163, 354, 1752, 2850
MspR9I	CCNGG	4 cut(s)	631, 826, 1753, 2850
MunI	CAATTG	2 cut(s)	1233, 2816
Mva1269I	GAATGC	3 cut(s)	280, 1203, 2350
MvaI	CCWGG	2 cut(s)	631, 826
MvnI	CGCG	1 cut(s)	864
NciI	CCSGG	2 cut(s)	1753, 2850
NcoI	CCATGG	1 cut(s)	285
NdeI	CATATG	1 cut(s)	1030
NlaIV	GGNNCC	3 cut(s)	358, 2733, 3030
NmuCI	GTSAC	3 cut(s)	1048, 2440, 2900
NsbI	TGCGCA	1 cut(s)	475
NsiI	ATGCAT	1 cut(s)	1506
NspI	RCATGY	6 cut(s)	422, 648, 1257, 1508, 1863, 2137
OliI	CACNNNNGTG	1 cut(s)	2447
PaeI	GCATGC	1 cut(s)	1257
PciI	ACATGT	2 cut(s)	418, 2133
PcsI	WCGNNNNNNNCGW	1 cut(s)	301
PctI	GAATGC	3 cut(s)	280, 1203, 2350
PdmI	GAANNNNTTC	1 cut(s)	876
Pfl23II	CGTACG	1 cut(s)	2751
PflFI	GACNNNGTC	1 cut(s)	1727
PflMI	CCANNNNNTGG	1 cut(s)	3000
PleI	GAGTC	5 cut(s)	37, 221, 1704, 2650, 2735
PpsI	GAGTC	5 cut(s)	37, 221, 1704, 2650, 2735
Ppu21I	YACGTR	3 cut(s)	296, 2755, 3107
PscI	ACATGT	2 cut(s)	418, 2133
PsiI	TTATAA	2 cut(s)	1131, 2216
Psp124BI	GAGCTC	2 cut(s)	173, 1576
Psp6I	CCWGG	2 cut(s)	629, 824
PspEI	GGTNACC	1 cut(s)	2900
PspFI	CCCAGC	1 cut(s)	400
PspGI	CCWGG	2 cut(s)	629, 824
PspLI	CGTACG	1 cut(s)	2751
PspN4I	GGNNCC	3 cut(s)	358, 2733, 3030
PspPI	GGNCC	1 cut(s)	1426
PsrI	GAACNNNNNNTAC	2 cut(s)	2572, 2604
PstI	CTGCAG	4 cut(s)	1278, 1720, 2094, 2155
PsuI	RGATCY	1 cut(s)	3028
PsyI	GACNNNGTC	1 cut(s)	1727
PvuII	CAGCTG	1 cut(s)	2966
RsaI	GTAC	9 cut(s)	718, 1532, 1561, 1997, 2457, 2753, 2774, 3158, 3192
RsaNI	GTAC	9 cut(s)	717, 1531, 1560, 1996, 2456, 2752, 2773, 3157, 3191
RseI	CAYNNNNRTG	9 cut(s)	243, 612, 893, 1033, 1116, 1629, 1658, 2376, 2447
SacI	GAGCTC	2 cut(s)	173, 1576
SaqAI	TTAA	7 cut(s)	335, 638, 972, 1629, 2174, 2258, 2663
Sau96I	GGNCC	1 cut(s)	1426
SchI	GAGTC	5 cut(s)	37, 221, 1704, 2650, 2735
ScrFI	CCNGG	4 cut(s)	631, 826, 1753, 2850
SduI	GDGCHC	6 cut(s)	173, 501, 1273, 1576, 1653, 2850
SfcI	CTRYAG	8 cut(s)	502, 771, 1274, 1642, 1716, 2090, 2151, 2467
SinI	GGWCC	1 cut(s)	1426
SmiMI	CAYNNNNRTG	9 cut(s)	243, 612, 893, 1033, 1116, 1629, 1658, 2376, 2447
SmlI	CTYRAG	2 cut(s)	121, 1602
SmoI	CTYRAG	2 cut(s)	121, 1602
SpeI	ACTAGT	2 cut(s)	188, 719
SphI	GCATGC	1 cut(s)	1257
SsiI	CCGC	8 cut(s)	86, 89, 456, 1063, 1161, 1313, 1689, 2959
SstI	GAGCTC	2 cut(s)	173, 1576
StyD4I	CCNGG	4 cut(s)	629, 824, 1751, 2848
StyI	CCWWGG	1 cut(s)	285
TaaI	ACNGT	3 cut(s)	1226, 3037, 3050
TaiI	ACGT	9 cut(s)	298, 626, 905, 1055, 1728, 2753, 2757, 3109, 3164
TaqI	TCGA	3 cut(s)	222, 3164, 3178
TaqII	GACCGA	1 cut(s)	2919
TatI	WGTACW	2 cut(s)	1530, 3156
TauI	GCSGC	2 cut(s)	91, 458
Tru1I	TTAA	7 cut(s)	335, 638, 972, 1629, 2174, 2258, 2663
Tru9I	TTAA	7 cut(s)	335, 638, 972, 1629, 2174, 2258, 2663
TscAI	CASTG	9 cut(s)	1143, 1163, 1720, 2094, 2362, 2634, 2695, 2784, 3055
TseFI	GTSAC	3 cut(s)	1048, 2440, 2900
Tsp45I	GTSAC	3 cut(s)	1048, 2440, 2900
TspGWI	ACGGA	1 cut(s)	2255
TspRI	CASTG	9 cut(s)	1143, 1163, 1720, 2094, 2362, 2634, 2695, 2784, 3055
Tth111I	GACNNNGTC	1 cut(s)	1727
Van91I	CCANNNNNTGG	1 cut(s)	3000
VpaK11BI	GGWCC	1 cut(s)	1426
XapI	RAATTY	6 cut(s)	272, 1092, 1698, 1896, 2875, 2936
XbaI	TCTAGA	1 cut(s)	2722
XceI	RCATGY	6 cut(s)	422, 648, 1257, 1508, 1863, 2137
XcmI	CCANNNNNNNNNTGG	1 cut(s)	568
XmnI	GAANNNNTTC	1 cut(s)	876
ZraI	GACGTC	1 cut(s)	1726
Zsp2I	ATGCAT	1 cut(s)	1506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.