Rh5CG346900

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0016595
Physical Location & Seq
Forward (+)
39980444 .. 39983749
3306 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG346900.1

Sequence Viewer

Length: 3234 bp
ATGAGTGGGAGTGGGTGTCTCAGGTCCTTGATGCTGCTCACTGTAAACCAATGCCACAACCCTATGTTCTTCCATCGCTACCGGCTTCATTTCAGAACCAAACCCAAACCCACCTCAGTTATCAATCTTCGCAAGCACTTTCGTGGGAGTGTAGTCAGTTTGAGCTTGGCGGAATATAGTAATGCAGCCAGTGCTCGTGTTTCGGATGATTATCTAGTTCATCAAAATGAAGGTGGTCCAAAGGGGATCGATTTGTTGCATTCTATGGAAGCACGTTGCATTCGTGCCAATTCTCAGACATATATTTGGCTTTTAAAAGGGTGCTTGAGTTCTGGGTCTTTGTTAGATGCTAGGAAACTTCACTCTAGGATTTTGAAGTTGGGGTTTGGTGGAGAATATGAAATAAGTGACCTGTTTGTTGGTGTTTATCTTGCAAATGGTGATTCATGCAGCGCAGTAAAGGTTTTCGATGATTTACCATATAGAAGTTTATTCTCATGGAATAACATAATTCATGGGTTTCTTGCAAAGAAGTTGACGGGTCAAGTATTGGGTTTCTTTTCCCAAATGGTAGCAGAAAATGTGCGTCCTGATGAGACCACGTTTGCAGGAGTTTTAAGGGCGTGTGGTGGTGGTAACGCCAGTATCCAATATGTGGAACAGATTCATGCTAGGATTATCCGCCATTGTTTTAGTACTAGTTTACTTGTATGTAATCCCTTGATCGATTTGTATGCTAAGAACGGCTCTGTGGATTCTGCTAAAAAAGTCTTTGATAAGTTGAATTTTTGGGATAGTGTTTCCTGGGTGGCTATAATTTCTGGCTTATCTCGTAATGGACGAGAAGAAGAAGCTGTTCTCCTATTCATCCAGATGCATACTTCAGGAATTTTCCCAACTCCTTATGTTTTCTCAAGCGTGATTAGTGCCTGTGCAAAAATAGAGTTATTTGAGCTGGGTGAGCAGCTGCAATGCCTTGTTTTCAAGGGAGGCTTTTCTTGTGAAACATACGTATGCAATGCTCTTGTAACATTATATTCCCGTTCAGGGAACTTCATATCTGCTGAACAAGTTTTCAATACAATGTGGCAAAGGGATGGAGTATCATATAACTCATTGATATCTGGGCTAGCTCAGTGTGGATTTAGTGATAGAGCTCTGGAATTGTTCAAGAAAATGCAGAGTGACTGTATGGAACCTGACTGTGTAACAATTGCTAGTCTTTTGAGTGCCTGTGCATCAGTTGGTTATCTGTATAAGGGGAAACAACTCCACTCATATGCAATTAAAGCAGGAATGTCATCAGATATAATCCTCGAAGGGTCTCTGCTTGATCTTTATGTGAAATGCTCAGATTTGCAAACCGCCTATGAATTTTTCCTTACAACAGAGACAGAGAATGTTGTCCTGTGGAATGTGATGCTAGTTGCTTATGGGCAGCAAGATGACCTGATAGAATCGTTTCATATTTTTAAACAAATGCATGTTGAGGGTATGATTCCTAATCAATATACCTATCCGAGTATTTTGAGAACCTGTACTTCTGTTGGAGCTCTCAATTTAGGAGAACAGGTTCATACCCAAGCCATAAAAACTGGCTTTCAGTTTAATGCATATGTCTGTAGCGTGCTCATTGATATGTATGCTAAGCATGGAAAACTGGATACTGCCCTTGGAATACTCAGAAGACTCACTGAAGATGATGTTGTTTCGTGGACAGCTATGATTGCAGGGTACGCACAGCATGATTTGTTTGCTGAAGCTCTTTTACTTTTTGAAGAAATGCAAAACCGAGGGATTCGATCTGACAACATAGGATTTTCAAGTGCAATTAGTTCATGTGCTGGTATTCAAGCACTCAATCAAGGTAGACAGATTCATGCTCAGTCATGCATATCTGGTTATTCGAATGATCTTTCCGTTGGCAATGCACTCGTTACCCTTTATGCTAGATGTGGTAGAATATGGGAAGCCTACCAAGCATTTGAGAAAATTGATGTGAAAGATAATATATCATGGAATGGACTGATATCAGGATTTGGACAGAGTGGGTACTGTGAGGAAGCACTGCAGGTATTTTCTCAGATGCATCGAGCTGGAGTAGAAGCTAATTTATTCACATTTGGCTCTGCAGTCAGCGCTGCTGCTAATTTAGCAAATATAAAACAAGGAGAGCAGATTCATGCCATGGTGATAAAAACTGGGAACAATTCAGAAGCTGAGGTTTCCAATGCTTTAATCACATTATACTCAAAGTGTGGTAGTGTTGATGATGCGGAGAGAGAGTTTATTGAAATGCCTGAAAAAAATGAGATTTCTTGGAATGCCATGATCACTGGCTATTCTCAGCATGGGCATGGTATTGAAGCACTACATCTATTTGAGCAGATGAAACAACTTGGTGTGGTGCCAAGCCATGTCACATTTGTGGGAGTACTTTCAGCTTGTAGCCATGTAGGCTTGGTAAGTGAGGGGCTTGCATACTTTGAATCCATGAGCAAAGAGCATGGACTAGTGCCTAAGCCTGAGCATTATGCTTGTGTTGTTGATCTTCTTAGTCGAGCTGGTTCTTTGAGCCAGGCAAGAAAATTCATATCTGAGATGCCAATAAAACCAGATTCAATGATTTGGAGGACTCTCTTAAGTGCTTGTATAGCTAAAAAGAACACAGAAATTGGGGAGGTTGCTGCCCGTCATCTTCTAGAACTGGAACCTGAAGACTCAGCAACTTACGTTCTCATATCAAACATGTATGCAGTGGCAGGAATGTGGGGTTATAGGGATCAGGCAAGGCAATTAATGAAGGAAAGGGGTGTGAAGAAAGAGCCTGGCCGCAGTTGGATTGAGGTAAAGAATTCGGTTCATGCATTCTATGTTGGTGACCGGCTGCACCCACTTGCCAATAAGATATATGAATTCTTAGGGGATTTGAATGAGCGGGCAGCTGAAATTGGTTATGTTGAGGACCGTAACAACCTCTGGAATGACATGGAACAACAGCATAAAGATCCAACAGTGTACATTCATAGCGAGAAATTGGCAATAGCTTTTGGCCTGATTAGCCTATCTAATACGATTCCCATACGTGTAATTAAGAATCTCCGTGTTTGTAATGATTGCCATAATTGGATCAAGCATACGTCGAAGATCTCAAAAAGAACTATCATAGTACGAGATGCGTATCGTTTTCATCATTTTAAAGATGGAGTTTGCTCTTGTAACGATTACTGGTAA

Protein Analysis

1077

Amino Acids

120.01

Weight (kDa)

6.38

Isoelectric Point (pI)

37.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 164 - 209 5.8e-07 PPR repeat family
PPR_2 PF13041 265 - 313 1.5e-07 PPR repeat family
PPR_2 PF13041 366 - 413 1.5e-08 PPR repeat family
PPR_1 PF12854 366 - 394 6.7e-06 PPR repeat
PPR PF01535 368 - 396 5.6e-08 PPR repeat
PPR_2 PF13041 467 - 513 1.5e-07 PPR repeat family
PPR_2 PF13041 568 - 615 2e-09 PPR repeat family
PPR PF01535 570 - 600 9.8e-08 PPR repeat
PPR_2 PF13041 669 - 713 9e-08 PPR repeat family
PPR PF01535 671 - 701 2.3e-07 PPR repeat
PPR_2 PF13041 770 - 817 4.6e-11 PPR repeat family
PPR PF01535 772 - 802 2.9e-08 PPR repeat
E_motif PF20431 889 - 949 4.5e-19 E motif
DYW_deaminase PF14432 985 - 1077 6.5e-34 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016595)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13650
fragaria_vesca FvH4_3g25260
malus_domestica MD12G1002600.v1.1
prunus_persica Prupe.7G002600_v2.0.a1
pyrus_communis pycom12g00230
rosa_chinensis RchiOBHm_Chr5g0046881
rosa_laevigata RLG00000034452
rosa_roxburghii Rroxscaffold_1G00034510
rosa_rugosa Rorug05G0232400
rosa_samantha Rh5AG311800 Rh5BG320800 Rh5CG346900 Rh5DG331500
rosa_wichuraiana Rw5G029140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 2062
AccB1I GGYRCC 1 cut(s) 2407
AccB7I CCANNNNNTGG 1 cut(s) 655
AccBSI CCGCTC 1 cut(s) 2938
AccI GTMKAC 1 cut(s) 1870
AciI CCGC 6 cut(s) 170, 682, 1365, 2276, 2833, 2938
AclWI GGATC 4 cut(s) 254, 2790, 3002, 3137
AcoI YGGCCR 1 cut(s) 2830
AcsI RAATTY 6 cut(s) 784, 888, 1373, 2588, 2854, 2915
AcuI CTGAAG 4 cut(s) 867, 1716, 1779, 2736
AfaI GTAC 7 cut(s) 697, 1540, 1736, 2054, 2436, 3020, 3171
AfeI AGCGCT 1 cut(s) 2140
AfiI CCNNNNNNNGG 2 cut(s) 655, 1047
AflII CTTAAG 1 cut(s) 2641
AflIII ACRYGT 2 cut(s) 2748, 3085
AhlI ACTAGT 2 cut(s) 698, 2512
AjnI CCWGG 3 cut(s) 803, 2577, 2827
AleI CACNNNNGTG 2 cut(s) 141, 2426
AloI GAACNNNNNNTCC 2 cut(s) 1557, 1589
Alw21I GWGCWC 4 cut(s) 196, 1159, 1555, 1632
Alw26I GTCTC 4 cut(s) 23, 590, 1329, 1385
AlwI GGATC 4 cut(s) 254, 2790, 3002, 3137
AlwNI CAGNNNCTG 1 cut(s) 2219
Aor51HI AGCGCT 1 cut(s) 2140
AoxI GGCC 2 cut(s) 2830, 3052
ApoI RAATTY 6 cut(s) 784, 888, 1373, 2588, 2854, 2915
AseI ATTAAT 1 cut(s) 2798
Asp700I GAANNNNTTC 4 cut(s) 663, 855, 1461, 1572
AspLEI GCGC 2 cut(s) 455, 2141
AspS9I GGNCC 3 cut(s) 24, 236, 2965
AsuHPI GGTGA 4 cut(s) 452, 971, 2203, 2891
AsuII TTCGAA 1 cut(s) 1907
AsuNHI GCTAGC 1 cut(s) 1129
AvaII GGWCC 3 cut(s) 24, 236, 2965
BaeI ACNNNNGTAYC 2 cut(s) 628, 661
BanI GGYRCC 1 cut(s) 2407
BanII GRGCYC 2 cut(s) 1159, 1555
BarI GAAGNNNNNNTAC 2 cut(s) 1752, 1784
BauI CACGAG 1 cut(s) 195
BbsI GAAGAC 2 cut(s) 1693, 2724
Bbv12I GWGCWC 4 cut(s) 196, 1159, 1555, 1632
BbvCI CCTCAGC 1 cut(s) 2220
BccI CCATC 3 cut(s) 81, 1091, 3197
BceAI ACGGC 1 cut(s) 760
BcgI CGANNNNNNTGC 4 cut(s) 716, 750, 1915, 1949
BciT130I CCWGG 3 cut(s) 805, 2579, 2829
BciVI GTATCC 2 cut(s) 656, 1657
BclI TGATCA 1 cut(s) 2331
BcoDI GTCTC 4 cut(s) 23, 590, 1329, 1385
BcuI ACTAGT 2 cut(s) 698, 2512
BfmI CTRYAG 3 cut(s) 1621, 2069, 2130
BfoI RGCGCY 1 cut(s) 2142
BfrI CTTAAG 1 cut(s) 2641
BfuAI ACCTGC 1 cut(s) 2062
BfuI GTATCC 2 cut(s) 656, 1657
BglI GCCNNNNNGGC 1 cut(s) 2457
BglII AGATCT 1 cut(s) 3147
BlpI GCTNAGC 1 cut(s) 1647
BmcAI AGTACT 2 cut(s) 697, 2436
Bme1390I CCNGG 3 cut(s) 805, 2579, 2829
Bme18I GGWCC 3 cut(s) 24, 236, 2965
BmgT120I GGNCC 3 cut(s) 24, 236, 2965
BmiI GGNNCC 3 cut(s) 1197, 2409, 2712
BmrFI CCNGG 3 cut(s) 805, 2579, 2829
BmrI ACTGGG 1 cut(s) 2211
BmtI GCTAGC 1 cut(s) 1133
BmuI ACTGGG 1 cut(s) 2211
BpiI GAAGAC 2 cut(s) 1693, 2724
BpmI CTGGAG 1 cut(s) 2118
Bpu10I CCTNAGC 3 cut(s) 2220, 2520, 2526
Bpu1102I GCTNAGC 1 cut(s) 1647
Bpu14I TTCGAA 1 cut(s) 1907
BpuEI CTTGAG 2 cut(s) 346, 898
Bsa29I ATCGAT 2 cut(s) 249, 726
BsaAI YACGTR 2 cut(s) 1012, 3086
BsaBI GATNNNNATC 2 cut(s) 210, 3180
BsaI GGTCTC 2 cut(s) 590, 1329
BsaJI CCNNGG 4 cut(s) 804, 1672, 1792, 2187
BsaXI ACNNNNNCTCC 5 cut(s) 31, 1557, 1587, 2270, 2300
Bsc4I CCNNNNNNNGG 2 cut(s) 655, 1047
Bse118I RCCGGY 2 cut(s) 81, 2883
Bse1I ACTGG 8 cut(s) 189, 642, 1600, 1665, 2206, 2341, 2712, 3233
Bse3DI GCAATG 3 cut(s) 977, 1024, 1933
Bse8I GATNNNNATC 2 cut(s) 210, 3180
BseBI CCWGG 3 cut(s) 805, 2579, 2829
BseCI ATCGAT 2 cut(s) 249, 726
BseDI CCNNGG 4 cut(s) 804, 1672, 1792, 2187
BseGI GGATG 3 cut(s) 211, 867, 1102
BseJI GATNNNNATC 2 cut(s) 210, 3180
BseLI CCNNNNNNNGG 2 cut(s) 655, 1047
BseMI GCAATG 3 cut(s) 977, 1024, 1933
BseNI ACTGG 8 cut(s) 189, 642, 1600, 1665, 2206, 2341, 2712, 3233
BseYI CCCAGC 1 cut(s) 955
BsgI GTGCAG 1 cut(s) 2873
BshFI GGCC 2 cut(s) 2832, 3054
BshNI GGYRCC 1 cut(s) 2407
BshVI ATCGAT 2 cut(s) 249, 726
BsiHKAI GWGCWC 4 cut(s) 196, 1159, 1555, 1632
BsiSI CCGG 2 cut(s) 82, 2884
BslI CCNNNNNNNGG 2 cut(s) 655, 1047
BsmAI GTCTC 4 cut(s) 23, 590, 1329, 1385
BsmI GAATGC 4 cut(s) 259, 279, 2329, 2867
BsnI GGCC 2 cut(s) 2832, 3054
Bso31I GGTCTC 2 cut(s) 590, 1329
Bsp119I TTCGAA 1 cut(s) 1907
Bsp1286I GDGCHC 4 cut(s) 196, 1159, 1555, 1632
Bsp1407I TGTACA 1 cut(s) 3018
Bsp1720I GCTNAGC 1 cut(s) 1647
Bsp19I CCATGG 1 cut(s) 2187
BspACI CCGC 6 cut(s) 170, 682, 1365, 2276, 2833, 2938
BspANI GGCC 2 cut(s) 2832, 3054
BspDI ATCGAT 2 cut(s) 249, 726
BspLI GGNNCC 3 cut(s) 1197, 2409, 2712
BspMAI CTGCAG 2 cut(s) 2073, 2134
BspMI ACCTGC 1 cut(s) 2062
BspOI GCTAGC 1 cut(s) 1133
BspPI GGATC 4 cut(s) 254, 2790, 3002, 3137
BspT104I TTCGAA 1 cut(s) 1907
BspT107I GGYRCC 1 cut(s) 2407
BspTI CTTAAG 1 cut(s) 2641
BspTNI GGTCTC 2 cut(s) 590, 1329
BsrBI CCGCTC 1 cut(s) 2938
BsrDI GCAATG 3 cut(s) 977, 1024, 1933
BsrFI RCCGGY 2 cut(s) 81, 2883
BsrGI TGTACA 1 cut(s) 3018
BsrI ACTGG 8 cut(s) 189, 642, 1600, 1665, 2206, 2341, 2712, 3233
BssAI RCCGGY 2 cut(s) 81, 2883
BssECI CCNNGG 4 cut(s) 804, 1672, 1792, 2187
BssSI CACGAG 1 cut(s) 195
BssT1I CCWWGG 2 cut(s) 1672, 2187
Bst2BI CACGAG 1 cut(s) 195
Bst2UI CCWGG 3 cut(s) 805, 2579, 2829
Bst4CI ACNGT 6 cut(s) 43, 1190, 1205, 2057, 2969, 3016
BstAFI CTTAAG 1 cut(s) 2641
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 3018
BstBAI YACGTR 2 cut(s) 1012, 3086
BstBI TTCGAA 1 cut(s) 1907
BstC8I GCNNGC 5 cut(s) 134, 1131, 1628, 2478, 2940
BstDSI CCRYGG 1 cut(s) 2187
BstEII GGTNACC 1 cut(s) 2879
BstF5I GGATG 3 cut(s) 211, 867, 1102
BstH2I RGCGCY 1 cut(s) 2142
BstHHI GCGC 2 cut(s) 455, 2141
BstMAI GTCTC 4 cut(s) 23, 590, 1329, 1385
BstNI CCWGG 3 cut(s) 805, 2579, 2829
BstNSI RCATGY 2 cut(s) 1487, 2752
BstPI GGTNACC 1 cut(s) 2879
BstSCI CCNGG 3 cut(s) 803, 2577, 2827
BstSFI CTRYAG 3 cut(s) 1621, 2069, 2130
BstSNI TACGTA 1 cut(s) 1012
BstV2I GAAGAC 2 cut(s) 1693, 2724
BstX2I RGATCY 2 cut(s) 3007, 3147
BstYI RGATCY 2 cut(s) 3007, 3147
Bsu15I ATCGAT 2 cut(s) 249, 726
BsuI GTATCC 2 cut(s) 656, 1657
BsuRI GGCC 2 cut(s) 2832, 3054
BsuTUI ATCGAT 2 cut(s) 249, 726
BtgI CCRYGG 1 cut(s) 2187
BtgZI GCGATG 1 cut(s) 59
BtsCI GGATG 3 cut(s) 211, 867, 1102
BtsI GCAGTG 2 cut(s) 2066, 2763
BtsIMutI CAGTG 8 cut(s) 39, 196, 1142, 1692, 2066, 2334, 2763, 3021
BveI ACCTGC 1 cut(s) 2062
Cac8I GCNNGC 5 cut(s) 134, 1131, 1628, 2478, 2940
CaiI CAGNNNCTG 1 cut(s) 2219
CfoI GCGC 2 cut(s) 455, 2141
Cfr10I RCCGGY 2 cut(s) 81, 2883
Cfr13I GGNCC 3 cut(s) 24, 236, 2965
ClaI ATCGAT 2 cut(s) 249, 726
CseI GACGC 1 cut(s) 575
Csp6I GTAC 7 cut(s) 696, 1539, 1735, 2053, 2435, 3019, 3170
CviQI GTAC 7 cut(s) 696, 1539, 1735, 2053, 2435, 3019, 3170
DraI TTTAAA 3 cut(s) 315, 1474, 3199
EaeI YGGCCR 1 cut(s) 2830
EciI GGCGGA 2 cut(s) 185, 671
Ecl136II GAGCTC 2 cut(s) 1157, 1553
Eco105I TACGTA 1 cut(s) 1012
Eco130I CCWWGG 2 cut(s) 1672, 2187
Eco24I GRGCYC 2 cut(s) 1159, 1555
Eco31I GGTCTC 2 cut(s) 590, 1329
Eco32I GATATC 2 cut(s) 1122, 2031
Eco47I GGWCC 3 cut(s) 24, 236, 2965
Eco47III AGCGCT 1 cut(s) 2140
Eco53kI GAGCTC 2 cut(s) 1157, 1553
Eco57I CTGAAG 4 cut(s) 867, 1716, 1779, 2736
Eco91I GGTNACC 1 cut(s) 2879
EcoICRI GAGCTC 2 cut(s) 1157, 1553
EcoO109I RGGNCCY 1 cut(s) 24
EcoO65I GGTNACC 1 cut(s) 2879
EcoRI GAATTC 2 cut(s) 2854, 2915
EcoRII CCWGG 3 cut(s) 803, 2577, 2827
EcoRV GATATC 2 cut(s) 1122, 2031
EcoT14I CCWWGG 2 cut(s) 1672, 2187
EcoT22I ATGCAT 6 cut(s) 879, 1485, 1615, 1895, 2091, 2869
EcoT38I GRGCYC 2 cut(s) 1159, 1555
ErhI CCWWGG 2 cut(s) 1672, 2187
FalI AAGNNNNNCTT 2 cut(s) 977, 1009
FauI CCCGC 1 cut(s) 2931
FauNDI CATATG 2 cut(s) 1279, 1615
FbaI TGATCA 1 cut(s) 2331
FblI GTMKAC 1 cut(s) 1870
FokI GGATG 3 cut(s) 218, 854, 1109
FriOI GRGCYC 2 cut(s) 1159, 1555
GlaI GCGC 2 cut(s) 454, 2140
GsaI CCCAGC 1 cut(s) 959
GsuI CTGGAG 1 cut(s) 2118
HaeII RGCGCY 1 cut(s) 2142
HaeIII GGCC 2 cut(s) 2832, 3054
HapII CCGG 2 cut(s) 82, 2884
HgaI GACGC 1 cut(s) 575
HhaI GCGC 2 cut(s) 455, 2141
Hin6I GCGC 2 cut(s) 453, 2139
HinP1I GCGC 2 cut(s) 453, 2139
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HpaII CCGG 2 cut(s) 82, 2884
HphI GGTGA 4 cut(s) 452, 971, 2203, 2891
Hpy166II GTNNAC 6 cut(s) 46, 537, 704, 1716, 1871, 3019
Hpy188III TCNNGA 8 cut(s) 590, 871, 885, 1160, 1171, 2034, 2702, 2980
Hpy8I GTNNAC 6 cut(s) 46, 537, 704, 1716, 1871, 3019
Hpy99I CGWCG 1 cut(s) 3145
HpyAV CCTTC 3 cut(s) 224, 1313, 2797
HpyCH4III ACNGT 6 cut(s) 43, 1190, 1205, 2057, 2969, 3016
HpyCH4IV ACGT 6 cut(s) 274, 602, 1011, 2733, 3085, 3140
HpySE526I ACGT 6 cut(s) 274, 602, 1011, 2733, 3085, 3140
HspAI GCGC 2 cut(s) 453, 2139
Ksp22I TGATCA 1 cut(s) 2331
LmnI GCTCC 1 cut(s) 1550
MaeII ACGT 6 cut(s) 274, 602, 1011, 2733, 3085, 3140
MbiI CCGCTC 1 cut(s) 2938
MfeI CAATTG 1 cut(s) 1212
MflI RGATCY 2 cut(s) 3007, 3147
MhlI GDGCHC 4 cut(s) 196, 1159, 1555, 1632
MlyI GAGTC 3 cut(s) 1683, 2629, 2714
MmeI TCCRAC 3 cut(s) 1528, 2819, 3035
Mph1103I ATGCAT 6 cut(s) 879, 1485, 1615, 1895, 2091, 2869
MroXI GAANNNNTTC 4 cut(s) 663, 855, 1461, 1572
MslI CAYNNNNRTG 8 cut(s) 141, 225, 872, 1012, 1278, 1637, 2355, 2426
MspA1I CMGCKG 2 cut(s) 967, 2945
MspCI CTTAAG 1 cut(s) 2641
MspI CCGG 2 cut(s) 82, 2884
MspR9I CCNGG 3 cut(s) 805, 2579, 2829
MunI CAATTG 1 cut(s) 1212
Mva1269I GAATGC 4 cut(s) 259, 279, 2329, 2867
MvaI CCWGG 3 cut(s) 805, 2579, 2829
NcoI CCATGG 1 cut(s) 2187
NdeI CATATG 2 cut(s) 1279, 1615
NheI GCTAGC 1 cut(s) 1129
NlaIV GGNNCC 3 cut(s) 1197, 2409, 2712
NmuCI GTSAC 4 cut(s) 407, 1184, 2419, 2879
NsiI ATGCAT 6 cut(s) 879, 1485, 1615, 1895, 2091, 2869
NspI RCATGY 2 cut(s) 1487, 2752
NspV TTCGAA 1 cut(s) 1907
OliI CACNNNNGTG 2 cut(s) 141, 2426
PciI ACATGT 1 cut(s) 2748
PcsI WCGNNNNNNNCGW 2 cut(s) 280, 838
PctI GAATGC 4 cut(s) 259, 279, 2329, 2867
PdmI GAANNNNTTC 4 cut(s) 663, 855, 1461, 1572
PflMI CCANNNNNTGG 1 cut(s) 655
PleI GAGTC 3 cut(s) 1683, 2629, 2714
PpsI GAGTC 3 cut(s) 1683, 2629, 2714
Ppu21I YACGTR 2 cut(s) 1012, 3086
PpuMI RGGWCCY 1 cut(s) 24
PscI ACATGT 1 cut(s) 2748
PshBI ATTAAT 1 cut(s) 2798
Psp124BI GAGCTC 2 cut(s) 1159, 1555
Psp5II RGGWCCY 1 cut(s) 24
Psp6I CCWGG 3 cut(s) 803, 2577, 2827
PspEI GGTNACC 1 cut(s) 2879
PspFI CCCAGC 1 cut(s) 955
PspGI CCWGG 3 cut(s) 803, 2577, 2827
PspN4I GGNNCC 3 cut(s) 1197, 2409, 2712
PspPI GGNCC 3 cut(s) 24, 236, 2965
PspPPI RGGWCCY 1 cut(s) 24
PstI CTGCAG 2 cut(s) 2073, 2134
PstNI CAGNNNCTG 1 cut(s) 2219
PsuI RGATCY 2 cut(s) 3007, 3147
PvuII CAGCTG 2 cut(s) 967, 2945
RsaI GTAC 7 cut(s) 697, 1540, 1736, 2054, 2436, 3020, 3171
RsaNI GTAC 7 cut(s) 696, 1539, 1735, 2053, 2435, 3019, 3170
RseI CAYNNNNRTG 8 cut(s) 141, 225, 872, 1012, 1278, 1637, 2355, 2426
SacI GAGCTC 2 cut(s) 1159, 1555
Sau96I GGNCC 3 cut(s) 24, 236, 2965
ScaI AGTACT 2 cut(s) 697, 2436
SchI GAGTC 3 cut(s) 1683, 2629, 2714
ScrFI CCNGG 3 cut(s) 805, 2579, 2829
SduI GDGCHC 4 cut(s) 196, 1159, 1555, 1632
SfcI CTRYAG 3 cut(s) 1621, 2069, 2130
SfuI TTCGAA 1 cut(s) 1907
SinI GGWCC 3 cut(s) 24, 236, 2965
SmiMI CAYNNNNRTG 8 cut(s) 141, 225, 872, 1012, 1278, 1637, 2355, 2426
SmlI CTYRAG 3 cut(s) 325, 913, 2641
SmoI CTYRAG 3 cut(s) 325, 913, 2641
SnaBI TACGTA 1 cut(s) 1012
SpeI ACTAGT 2 cut(s) 698, 2512
SsiI CCGC 6 cut(s) 170, 682, 1365, 2276, 2833, 2938
SstI GAGCTC 2 cut(s) 1159, 1555
StyD4I CCNGG 3 cut(s) 803, 2577, 2827
StyI CCWWGG 2 cut(s) 1672, 2187
TaaI ACNGT 6 cut(s) 43, 1190, 1205, 2057, 2969, 3016
TaiI ACGT 6 cut(s) 277, 605, 1014, 2736, 3088, 3143
TaqI TCGA 9 cut(s) 249, 468, 726, 1317, 1801, 1907, 2092, 2560, 3143
TatI WGTACW 4 cut(s) 695, 1538, 2434, 3018
TauI GCSGC 1 cut(s) 2835
TscAI CASTG 8 cut(s) 46, 196, 1142, 1699, 2073, 2341, 2763, 3021
TseFI GTSAC 4 cut(s) 407, 1184, 2419, 2879
Tsp45I GTSAC 4 cut(s) 407, 1184, 2419, 2879
TspGWI ACGGA 2 cut(s) 1909, 3092
TspRI CASTG 8 cut(s) 46, 196, 1142, 1699, 2073, 2341, 2763, 3021
Van91I CCANNNNNTGG 1 cut(s) 655
Vha464I CTTAAG 1 cut(s) 2641
VpaK11BI GGWCC 3 cut(s) 24, 236, 2965
VspI ATTAAT 1 cut(s) 2798
XapI RAATTY 6 cut(s) 784, 888, 1373, 2588, 2854, 2915
XbaI TCTAGA 1 cut(s) 2701
XceI RCATGY 2 cut(s) 1487, 2752
XmiI GTMKAC 1 cut(s) 1870
XmnI GAANNNNTTC 4 cut(s) 663, 855, 1461, 1572
ZrmI AGTACT 2 cut(s) 697, 2436
Zsp2I ATGCAT 6 cut(s) 879, 1485, 1615, 1895, 2091, 2869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.