FvH4_3g27770

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0004092
Physical Location & Seq
Reverse (-)
20803218 .. 20814103
10886 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27770.t6

Sequence Viewer

Length: 1602 bp
ATGGATTCCAGCAGCAGCCTTGTTCATCATCAACTGCTCTTCTCTCTCCCCATCATCATCATCCTTCTCTTGGCCCTTCCTTGGACCTCCGAGCCCAGCCAGTTCTGCAACTCTGGAATTAGATATGGTTGTGAGGTTTCGTCCAAGTTGTTGATCAAAGGAGGGTCTGTAGTCAATGCTCATCACATGGAGATTGCTGATGTTTATGTCGAGGATGGAATCATTGTTGCTGTCAGCCCCAATATCAAGGTTGGTGACGATGTTACTGTGCTTGATGCCAGTGGAAAGTTTGTCATGCCAGGAGGAATTGATCCCCATACTCACCTAGCCATGGAGTTCATGGGAACAGAAACTATTGATGATTTCTTCAGTGGTCAGGCTGCAGCATTAGCAGGTGGAACAACTATGCACATTGACTTTGTCATACCAGTTAATGGAAGTTTAACGGGAGGGCTGGAAGCTTATGAAAAAAAATCTGGGAAGTCCTGCATGGATTATGGTTTCCATATGGCAATCACTAAGTGGGATGAAATTGTTTCTCAGGAAATGGAAATAATCGTTAAAGAGAAAGGTATAAACTCTTTCAAGTTTTTCCTGGCCTACAAGGGGTCTCTTATGATAAACGATGAGCTTCTGTTACAAGGATTCAAAAAGTGTAAGTCTCTTGGTGCTTTAGCTATGGTGCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAAAAGAATGAAAGAGCTTGGCATAACTGGTCCAGAGGGACATTCTCTTTCAAGACCCCCAGTGCTGGAAGGTGAGGCCACCGCTCGAGCAATTCGATTAGCAGCTTTTGTGAATACCCCCCTCTATGTAGTTCATGTGATGAGCATTGATGCCATGGAAGAAATTTCTAAAGCTCGCAAATCAGGGATTAAGGTCATTGGCGAACCAGTAGTCTCTGGTTTAGTGCTGAATGAATCTTGGCTTTGGAATCCTGACTTTATCACTGCTGCTAAGTATGTGATGAGTCCCCCTATCAGGGCTGCAGGACATGACAGAGCCCTCCAAGCTGCTCTTTCGATGGGAATTTTACAGCTGGTAGGAACTGATCATTGTACCTTCAATTCTACACAGAAAGCTCTTGGAATTGATGATTTCCAGAAAATTCCGAATGGTGTTAATGGTATTGAGGAGAGGATGCATGTCCTATGGGATACAATGGTGGAATCTGGTCAAATTTCTATCACTGACTATGTCCGCTTGACAAGCGCTGAATGTGCTAAGATTTTCAATATATATCCGAGGAAGGGAGCAATTCGTGCTGGATCGGATGCAGATATAATTATATTAAATCCAAACGCAAGCTTTGAAATAAGTGCAAAGTCCCATCACTCTAGATCTGATACCAATGTCTACGAGGGATGGAGAGGGAAGGGCAAGGTTGAGGTCACTATTGCTGGTGGAAGAGTTGTTTGGCAAAATGAAGAGCTGAATATTGTTCCTGGATCCGGAAAGTACATAGAAATGCCTCCTTTCAGTTATCTTTTTGATGGAATGGATAAGGCCGATGCAAACTACATATCATCTCTCAAGGCTCCGGTAAAGCGTTTAAGAACCGCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

534

Amino Acids

58.02

Weight (kDa)

5.76

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 97 - 452 1.3e-23 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 383
Acc36I ACCTGC 1 cut(s) 383
AccB7I CCANNNNNTGG 1 cut(s) 434
AccBSI CCGCTC 1 cut(s) 803
AccI GTMKAC 1 cut(s) 1389
AccIII TCCGGA 1 cut(s) 1484
AciI CCGC 3 cut(s) 801, 1234, 1593
AclWI GGATC 4 cut(s) 305, 1309, 1476, 1489
AcsI RAATTY 4 cut(s) 882, 1062, 1140, 1212
AcuI CTGAAG 1 cut(s) 352
AdeI CACNNNGTG 1 cut(s) 522
AfaI GTAC 2 cut(s) 1093, 1493
AfeI AGCGCT 1 cut(s) 1246
AgsI TTSAA 6 cut(s) 586, 649, 771, 1099, 1267, 1346
AjnI CCWGG 3 cut(s) 298, 594, 1477
AjuI GAANNNNNNNTTGG 2 cut(s) 1432, 1464
Alw26I GTCTC 3 cut(s) 615, 666, 937
AlwI GGATC 4 cut(s) 305, 1309, 1476, 1489
Ama87I CYCGRG 1 cut(s) 804
Aor13HI TCCGGA 1 cut(s) 1484
Aor51HI AGCGCT 1 cut(s) 1246
AoxI GGCC 4 cut(s) 72, 597, 795, 1539
ApeKI GCWGC 8 cut(s) 12, 15, 380, 383, 821, 986, 1019, 1046
ApoI RAATTY 4 cut(s) 882, 1062, 1140, 1212
ArsI GACNNNNNNTTYG 2 cut(s) 915, 947
AspLEI GCGC 1 cut(s) 1247
AspS9I GGNCC 3 cut(s) 73, 84, 749
AsuHPI GGTGA 3 cut(s) 266, 314, 803
AvaI CYCGRG 1 cut(s) 804
AvaII GGWCC 2 cut(s) 84, 749
BaeI ACNNNNGTAYC 4 cut(s) 1182, 1215, 1371, 1404
BamHI GGATCC 1 cut(s) 1481
BanII GRGCYC 2 cut(s) 96, 1039
BbvI GCAGC 8 cut(s) 24, 27, 367, 395, 833, 973, 1006, 1033
BccI CCATC 6 cut(s) 59, 209, 1051, 1371, 1392, 1520
BciT130I CCWGG 3 cut(s) 300, 596, 1479
BciVI GTATCC 1 cut(s) 1183
BclI TGATCA 2 cut(s) 153, 1084
BcoDI GTCTC 3 cut(s) 615, 666, 937
BfaI CTAG 2 cut(s) 326, 1371
BfmI CTRYAG 3 cut(s) 168, 381, 1020
BfoI RGCGCY 1 cut(s) 1248
BfuAI ACCTGC 1 cut(s) 383
BfuI GTATCC 1 cut(s) 1183
BglII AGATCT 1 cut(s) 1373
BisI GCNGC 8 cut(s) 13, 16, 381, 384, 822, 987, 1020, 1047
BlsI GCNGC 8 cut(s) 14, 17, 382, 385, 823, 988, 1021, 1048
Bme1390I CCNGG 3 cut(s) 300, 596, 1479
Bme18I GGWCC 2 cut(s) 84, 749
BmeT110I CYCGRG 1 cut(s) 804
BmgT120I GGNCC 3 cut(s) 73, 84, 749
BmiI GGNNCC 2 cut(s) 1483, 1572
BmrFI CCNGG 3 cut(s) 300, 596, 1479
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 6 cut(s) 265, 691, 859, 1164, 1297, 1534
BmuI ACTGGG 1 cut(s) 773
BpuEI CTTGAG 1 cut(s) 1550
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 4 cut(s) 80, 330, 873, 1277
BsaWI WCCGGW 2 cut(s) 1484, 1573
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 690, 720
Bse1I ACTGG 6 cut(s) 100, 279, 428, 751, 779, 926
BseAI TCCGGA 1 cut(s) 1484
BseBI CCWGG 3 cut(s) 300, 596, 1479
BseDI CCNNGG 4 cut(s) 80, 330, 873, 1277
BseGI GGATG 6 cut(s) 60, 220, 532, 1179, 1312, 1403
BseMII CTCAG 1 cut(s) 554
BseNI ACTGG 6 cut(s) 100, 279, 428, 751, 779, 926
BseRI GAGGAG 1 cut(s) 1181
BseXI GCAGC 8 cut(s) 24, 27, 367, 395, 833, 973, 1006, 1033
BseYI CCCAGC 1 cut(s) 95
BshFI GGCC 4 cut(s) 74, 599, 797, 1541
BsiHKCI CYCGRG 1 cut(s) 804
BsiSI CCGG 2 cut(s) 1485, 1574
BslFI GGGAC 3 cut(s) 771, 990, 1345
BsmAI GTCTC 3 cut(s) 615, 666, 937
BsmFI GGGAC 3 cut(s) 771, 990, 1345
BsnI GGCC 4 cut(s) 74, 599, 797, 1541
Bso31I GGTCTC 1 cut(s) 615
BsoBI CYCGRG 1 cut(s) 804
Bsp1286I GDGCHC 2 cut(s) 96, 1039
Bsp13I TCCGGA 1 cut(s) 1484
Bsp143I GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
Bsp19I CCATGG 2 cut(s) 330, 873
BspACI CCGC 3 cut(s) 801, 1234, 1593
BspANI GGCC 4 cut(s) 74, 599, 797, 1541
BspCNI CTCAG 1 cut(s) 553
BspEI TCCGGA 1 cut(s) 1484
BspLI GGNNCC 2 cut(s) 1483, 1572
BspMAI CTGCAG 2 cut(s) 385, 1024
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 4 cut(s) 305, 1309, 1476, 1489
BspQI GCTCTTC 2 cut(s) 44, 1455
BspTNI GGTCTC 1 cut(s) 615
BsrBI CCGCTC 1 cut(s) 803
BsrI ACTGG 6 cut(s) 100, 279, 428, 751, 779, 926
BssECI CCNNGG 4 cut(s) 80, 330, 873, 1277
BssMI GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
BssT1I CCWWGG 3 cut(s) 80, 330, 873
Bst2UI CCWGG 3 cut(s) 300, 596, 1479
Bst4CI ACNGT 1 cut(s) 268
Bst6I CTCTTC 3 cut(s) 44, 1435, 1455
BstAPI GCANNNNNTGC 1 cut(s) 1295
BstC8I GCNNGC 3 cut(s) 687, 895, 1339
BstDEI CTNAG 5 cut(s) 519, 540, 990, 1257, 1599
BstDSI CCRYGG 2 cut(s) 330, 873
BstF5I GGATG 6 cut(s) 60, 220, 532, 1179, 1312, 1403
BstH2I RGCGCY 1 cut(s) 1248
BstHHI GCGC 1 cut(s) 1247
BstKTI GATC 6 cut(s) 156, 313, 1087, 1304, 1376, 1484
BstMAI GTCTC 3 cut(s) 615, 666, 937
BstMBI GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
BstMWI GCNNNNNNNGC 6 cut(s) 105, 389, 1043, 1242, 1253, 1295
BstNI CCWGG 3 cut(s) 300, 596, 1479
BstNSI RCATGY 2 cut(s) 689, 1181
BstSCI CCNGG 3 cut(s) 298, 594, 1477
BstSFI CTRYAG 3 cut(s) 168, 381, 1020
BstV1I GCAGC 8 cut(s) 24, 27, 367, 395, 833, 973, 1006, 1033
BstX2I RGATCY 2 cut(s) 1373, 1481
BstYI RGATCY 2 cut(s) 1373, 1481
BsuI GTATCC 1 cut(s) 1183
BsuRI GGCC 4 cut(s) 74, 599, 797, 1541
BtgI CCRYGG 2 cut(s) 330, 873
BtsCI GGATG 6 cut(s) 60, 220, 532, 1179, 1312, 1403
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 5 cut(s) 286, 376, 786, 981, 1221
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 3 cut(s) 687, 895, 1339
CfoI GCGC 1 cut(s) 1247
Cfr13I GGNCC 3 cut(s) 73, 84, 749
Csp6I GTAC 2 cut(s) 1092, 1492
CviQI GTAC 2 cut(s) 1092, 1492
DdeI CTNAG 5 cut(s) 519, 540, 990, 1257, 1599
DpnI GATC 6 cut(s) 155, 312, 1086, 1303, 1375, 1483
DpnII GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
DraIII CACNNNGTG 1 cut(s) 522
Eam1104I CTCTTC 3 cut(s) 44, 1435, 1455
EarI CTCTTC 3 cut(s) 44, 1435, 1455
Eco130I CCWWGG 3 cut(s) 80, 330, 873
Eco24I GRGCYC 2 cut(s) 96, 1039
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 2 cut(s) 84, 749
Eco47III AGCGCT 1 cut(s) 1246
Eco57I CTGAAG 1 cut(s) 352
Eco88I CYCGRG 1 cut(s) 804
EcoRII CCWGG 3 cut(s) 298, 594, 1477
EcoT14I CCWWGG 3 cut(s) 80, 330, 873
EcoT22I ATGCAT 1 cut(s) 1179
EcoT38I GRGCYC 2 cut(s) 96, 1039
ErhI CCWWGG 3 cut(s) 80, 330, 873
FalI AAGNNNNNCTT 2 cut(s) 1035, 1067
FaqI GGGAC 3 cut(s) 771, 990, 1345
FauNDI CATATG 1 cut(s) 507
FbaI TGATCA 2 cut(s) 153, 1084
FblI GTMKAC 1 cut(s) 1389
Fnu4HI GCNGC 8 cut(s) 13, 16, 381, 384, 822, 987, 1020, 1047
FokI GGATG 6 cut(s) 47, 227, 539, 1186, 1319, 1410
FriOI GRGCYC 2 cut(s) 96, 1039
Fsp4HI GCNGC 8 cut(s) 13, 16, 381, 384, 822, 987, 1020, 1047
FspBI CTAG 2 cut(s) 326, 1371
GlaI GCGC 1 cut(s) 1246
GluI GCNGC 8 cut(s) 13, 16, 381, 384, 822, 987, 1020, 1047
GsaI CCCAGC 1 cut(s) 99
HaeII RGCGCY 1 cut(s) 1248
HaeIII GGCC 4 cut(s) 74, 599, 797, 1541
HapII CCGG 2 cut(s) 1485, 1574
HhaI GCGC 1 cut(s) 1247
Hin6I GCGC 1 cut(s) 1245
HinP1I GCGC 1 cut(s) 1245
HindIII AAGCTT 2 cut(s) 459, 1339
HinfI GANTC 7 cut(s) 5, 219, 645, 953, 967, 1003, 1202
HpaII CCGG 2 cut(s) 1485, 1574
HphI GGTGA 3 cut(s) 266, 314, 803
Hpy166II GTNNAC 1 cut(s) 1390
Hpy188I TCNGA 5 cut(s) 91, 1146, 1278, 1306, 1378
Hpy188III TCNNGA 8 cut(s) 114, 542, 752, 771, 971, 1135, 1371, 1485
Hpy8I GTNNAC 1 cut(s) 1390
HpyAV CCTTC 7 cut(s) 74, 86, 707, 782, 1105, 1276, 1402
HpyCH4III ACNGT 1 cut(s) 268
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 389, 1043, 1242, 1253, 1295
HpyF3I CTNAG 5 cut(s) 519, 540, 990, 1257, 1599
HspAI GCGC 1 cut(s) 1245
Kpn2I TCCGGA 1 cut(s) 1484
Ksp22I TGATCA 2 cut(s) 153, 1084
Kzo9I GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
LguI GCTCTTC 2 cut(s) 44, 1455
LmnI GCTCC 2 cut(s) 1286, 1576
Lsp1109I GCAGC 8 cut(s) 24, 27, 367, 395, 833, 973, 1006, 1033
LweI GCATC 6 cut(s) 265, 691, 859, 1164, 1297, 1534
MaeI CTAG 2 cut(s) 326, 1371
MaeIII GTNAC 4 cut(s) 254, 262, 636, 1423
MalI GATC 6 cut(s) 155, 312, 1086, 1303, 1375, 1483
MbiI CCGCTC 1 cut(s) 803
MboI GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
MboII GAAGA 5 cut(s) 31, 358, 890, 1452, 1472
MflI RGATCY 2 cut(s) 1373, 1481
MhlI GDGCHC 2 cut(s) 96, 1039
MlyI GAGTC 1 cut(s) 1012
Mph1103I ATGCAT 1 cut(s) 1179
MroI TCCGGA 1 cut(s) 1484
MseI TTAA 7 cut(s) 432, 443, 561, 909, 1155, 1325, 1586
MslI CAYNNNNRTG 1 cut(s) 1499
MspA1I CMGCKG 1 cut(s) 1072
MspI CCGG 2 cut(s) 1485, 1574
MspR9I CCNGG 3 cut(s) 300, 596, 1479
MvaI CCWGG 3 cut(s) 300, 596, 1479
MwoI GCNNNNNNNGC 6 cut(s) 105, 389, 1043, 1242, 1253, 1295
NcoI CCATGG 2 cut(s) 330, 873
NdeI CATATG 1 cut(s) 507
NdeII GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
NlaIV GGNNCC 2 cut(s) 1483, 1572
NmuCI GTSAC 2 cut(s) 254, 1423
NsiI ATGCAT 1 cut(s) 1179
NspI RCATGY 2 cut(s) 689, 1181
PaeI GCATGC 1 cut(s) 689
PaeR7I CTCGAG 1 cut(s) 804
PaqCI CACCTGC 1 cut(s) 383
PciSI GCTCTTC 2 cut(s) 44, 1455
PcsI WCGNNNNNNNCGW 1 cut(s) 811
PfeI GAWTC 6 cut(s) 5, 219, 645, 953, 967, 1202
PflFI GACNNNGTC 2 cut(s) 419, 1229
PflMI CCANNNNNTGG 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 1477
PkrI GCNGC 8 cut(s) 14, 17, 382, 385, 823, 988, 1021, 1048
PleI GAGTC 1 cut(s) 1011
PpsI GAGTC 1 cut(s) 1011
Psp6I CCWGG 3 cut(s) 298, 594, 1477
PspFI CCCAGC 1 cut(s) 95
PspGI CCWGG 3 cut(s) 298, 594, 1477
PspN4I GGNNCC 2 cut(s) 1483, 1572
PspPI GGNCC 3 cut(s) 73, 84, 749
PspXI VCTCGAGB 1 cut(s) 804
PstI CTGCAG 2 cut(s) 385, 1024
PsuI RGATCY 2 cut(s) 1373, 1481
PsyI GACNNNGTC 2 cut(s) 419, 1229
PvuII CAGCTG 1 cut(s) 1072
RsaI GTAC 2 cut(s) 1093, 1493
RsaNI GTAC 2 cut(s) 1092, 1492
RseI CAYNNNNRTG 1 cut(s) 1499
SapI GCTCTTC 2 cut(s) 44, 1455
SaqAI TTAA 7 cut(s) 432, 443, 561, 909, 1155, 1325, 1586
SatI GCNGC 8 cut(s) 13, 16, 381, 384, 822, 987, 1020, 1047
Sau3AI GATC 6 cut(s) 153, 310, 1084, 1301, 1373, 1481
Sau96I GGNCC 3 cut(s) 73, 84, 749
SchI GAGTC 1 cut(s) 1012
ScrFI CCNGG 3 cut(s) 300, 596, 1479
SduI GDGCHC 2 cut(s) 96, 1039
SfaNI GCATC 6 cut(s) 265, 691, 859, 1164, 1297, 1534
SfcI CTRYAG 3 cut(s) 168, 381, 1020
Sfr274I CTCGAG 1 cut(s) 804
SinI GGWCC 2 cut(s) 84, 749
SlaI CTCGAG 1 cut(s) 804
SmiMI CAYNNNNRTG 1 cut(s) 1499
SmlI CTYRAG 2 cut(s) 804, 1565
SmoI CTYRAG 2 cut(s) 804, 1565
SphI GCATGC 1 cut(s) 689
SsiI CCGC 3 cut(s) 801, 1234, 1593
SspI AATATT 1 cut(s) 1471
SspMI CTAG 2 cut(s) 326, 1371
StyD4I CCNGG 3 cut(s) 298, 594, 1477
StyI CCWWGG 3 cut(s) 80, 330, 873
TaaI ACNGT 1 cut(s) 268
TaqI TCGA 4 cut(s) 210, 805, 814, 1055
TatI WGTACW 1 cut(s) 1491
TfiI GAWTC 6 cut(s) 5, 219, 645, 953, 967, 1202
Tru1I TTAA 7 cut(s) 432, 443, 561, 909, 1155, 1325, 1586
Tru9I TTAA 7 cut(s) 432, 443, 561, 909, 1155, 1325, 1586
TscAI CASTG 5 cut(s) 286, 376, 786, 988, 1228
TseFI GTSAC 2 cut(s) 254, 1423
TseI GCWGC 8 cut(s) 12, 15, 380, 383, 821, 986, 1019, 1046
Tsp45I GTSAC 2 cut(s) 254, 1423
TspDTI ATGAA 9 cut(s) 14, 328, 480, 543, 726, 743, 842, 966, 1473
TspRI CASTG 5 cut(s) 286, 376, 786, 988, 1228
Tth111I GACNNNGTC 2 cut(s) 419, 1229
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 2 cut(s) 84, 749
XapI RAATTY 4 cut(s) 882, 1062, 1140, 1212
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 2 cut(s) 689, 1181
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XhoI CTCGAG 1 cut(s) 804
XmiI GTMKAC 1 cut(s) 1389
XspI CTAG 2 cut(s) 326, 1371
Zsp2I ATGCAT 1 cut(s) 1179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.