Rroxscaffold_1G00030540

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0004092
Physical Location & Seq
Forward (+)
40524467 .. 40531533
7067 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00030540.1

Sequence Viewer

Length: 810 bp
ATGGACTCCGGCCTTCATCAACTGCTCTTCCCTCTTTTATCTCTCCTCCTTCTCTTCGCCTTTCCTTCGACCTCCGAACCCGGCCAGTTCTGCAATTCTGGATTTGGATATGGCGATTTCGCCTGTGAGGTTTCGTCGTCGTCCAAGTTGTTGATCAAAGGAGGGACTGTAGTGAATGCCCACCACATGGAGATTGCTGATGTTTATGTGGAGGATGGAATCATTGTTGCTGTCAGCCCCAATATCAAGGTTGGTGATGATGTTACTGTGCTTGATGCCAGTAGAAAGTTTGTCATGCCAGGAGGAATCGATCCCCATACTCACCTAGCCATGGAGTTCATGGGTACAGAAACTATTGATGATTTCTTCAGTGGTCAAGCTGCAGCATTAGCAGGTGGAACAACTATGCACATTGACTTTGTTATACCAGTAAATGGAAGTTTAACGGCAGGGTTGGAAGCTTATGAAAAAAAATCTGGGAAGTCCTGTATGGATTATGGCTTCCATATGGCAATTACTAAATGGGATGAAATTGTTTCACAAGAAATGGAAATAATGGTTAAAGAGAAAGGTATAAACTCTTTCAAGTTTTTCCTGGCCTACAAGGGGTCTCTTATGATCAACGATGAGCTTCTACTACAAGGATTCAAAAAGTGCAAGTCTCTTGGTGCTTTAGCTATGGTGCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAAAAGAATGAAAGAACTTGGTATTACTGGTCCAGAGGGGCATTCTCTTTCAAGACCCCCAGTGGGCTTAAGGTCATTGGTGAGCCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

269

Amino Acids

29.04

Weight (kDa)

5.23

Isoelectric Point (pI)

32.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 97 - 238 1.2e-08 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 383
Acc36I ACCTGC 1 cut(s) 383
AccB7I CCANNNNNTGG 2 cut(s) 187, 434
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 1 cut(s) 82
AcuI CTGAAG 1 cut(s) 352
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 5 cut(s) 187, 331, 434, 606, 782
AflII CTTAAG 1 cut(s) 787
AgsI TTSAA 3 cut(s) 586, 649, 771
AjnI CCWGG 2 cut(s) 298, 594
AluBI AGCT 4 cut(s) 380, 461, 631, 677
AluI AGCT 4 cut(s) 380, 461, 631, 677
Alw26I GTCTC 2 cut(s) 615, 666
AlwI GGATC 1 cut(s) 305
AoxI GGCC 3 cut(s) 10, 82, 597
ApeKI GCWGC 2 cut(s) 380, 383
AspS9I GGNCC 1 cut(s) 749
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 266, 314
AvaII GGWCC 1 cut(s) 749
BbvI GCAGC 2 cut(s) 367, 395
BccI CCATC 1 cut(s) 209
BceAI ACGGC 1 cut(s) 462
BciT130I CCWGG 2 cut(s) 300, 596
BclI TGATCA 2 cut(s) 153, 618
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 2 cut(s) 615, 666
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 168, 381
BfrI CTTAAG 1 cut(s) 787
BfuAI ACCTGC 1 cut(s) 383
BisI GCNGC 2 cut(s) 381, 384
BlsI GCNGC 2 cut(s) 382, 385
Bme1390I CCNGG 3 cut(s) 81, 300, 596
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 1 cut(s) 749
BmrFI CCNGG 3 cut(s) 81, 300, 596
BmrI ACTGGG 1 cut(s) 773
BmsI GCATC 2 cut(s) 265, 691
BmuI ACTGGG 1 cut(s) 773
BpuMI CCSGG 1 cut(s) 81
Bsa29I ATCGAT 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 615
BsaJI CCNNGG 1 cut(s) 330
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 690, 720
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 331, 434, 606, 782
Bse1I ACTGG 6 cut(s) 85, 279, 428, 751, 779, 805
BseBI CCWGG 2 cut(s) 300, 596
BseCI ATCGAT 1 cut(s) 309
BseDI CCNNGG 1 cut(s) 330
BseGI GGATG 2 cut(s) 220, 532
BseLI CCNNNNNNNGG 5 cut(s) 187, 331, 434, 606, 782
BseNI ACTGG 6 cut(s) 85, 279, 428, 751, 779, 805
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 2 cut(s) 367, 395
BshFI GGCC 3 cut(s) 12, 84, 599
BshVI ATCGAT 1 cut(s) 309
BsiSI CCGG 2 cut(s) 9, 81
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 5 cut(s) 187, 331, 434, 606, 782
BsmAI GTCTC 2 cut(s) 615, 666
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 2 cut(s) 181, 760
BsnI GGCC 3 cut(s) 12, 84, 599
Bso31I GGTCTC 1 cut(s) 615
Bsp143I GATC 3 cut(s) 153, 310, 618
Bsp19I CCATGG 1 cut(s) 330
BspANI GGCC 3 cut(s) 12, 84, 599
BspDI ATCGAT 1 cut(s) 309
BspMAI CTGCAG 1 cut(s) 385
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 1 cut(s) 305
BspQI GCTCTTC 1 cut(s) 32
BspTI CTTAAG 1 cut(s) 787
BspTNI GGTCTC 1 cut(s) 615
BsrI ACTGG 6 cut(s) 85, 279, 428, 751, 779, 805
BssECI CCNNGG 1 cut(s) 330
BssMI GATC 3 cut(s) 153, 310, 618
BssT1I CCWWGG 1 cut(s) 330
Bst2UI CCWGG 2 cut(s) 300, 596
Bst4CI ACNGT 2 cut(s) 169, 268
Bst6I CTCTTC 2 cut(s) 32, 59
BstAFI CTTAAG 1 cut(s) 787
BstC8I GCNNGC 1 cut(s) 687
BstDSI CCRYGG 1 cut(s) 330
BstF5I GGATG 2 cut(s) 220, 532
BstKTI GATC 3 cut(s) 156, 313, 621
BstMAI GTCTC 2 cut(s) 615, 666
BstMBI GATC 3 cut(s) 153, 310, 618
BstMWI GCNNNNNNNGC 2 cut(s) 90, 389
BstNI CCWGG 2 cut(s) 300, 596
BstNSI RCATGY 1 cut(s) 689
BstSCI CCNGG 3 cut(s) 79, 298, 594
BstSFI CTRYAG 2 cut(s) 168, 381
BstV1I GCAGC 2 cut(s) 367, 395
Bsu15I ATCGAT 1 cut(s) 309
BsuRI GGCC 3 cut(s) 12, 84, 599
BsuTUI ATCGAT 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 330
BtsCI GGATG 2 cut(s) 220, 532
BtsIMutI CAGTG 2 cut(s) 376, 786
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 1 cut(s) 687
Cfr13I GGNCC 1 cut(s) 749
ClaI ATCGAT 1 cut(s) 309
Csp6I GTAC 1 cut(s) 345
CviAII CATG 5 cut(s) 187, 295, 331, 340, 686
CviQI GTAC 1 cut(s) 345
DpnI GATC 3 cut(s) 155, 312, 620
DpnII GATC 3 cut(s) 153, 310, 618
EaeI YGGCCR 1 cut(s) 82
Eam1104I CTCTTC 2 cut(s) 32, 59
EarI CTCTTC 2 cut(s) 32, 59
Eco130I CCWWGG 1 cut(s) 330
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 352
EcoRII CCWGG 2 cut(s) 298, 594
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 5 cut(s) 190, 298, 334, 343, 689
FaqI GGGAC 1 cut(s) 178
FatI CATG 5 cut(s) 186, 294, 330, 339, 685
FauNDI CATATG 1 cut(s) 507
FbaI TGATCA 2 cut(s) 153, 618
Fnu4HI GCNGC 2 cut(s) 381, 384
FokI GGATG 2 cut(s) 227, 539
Fsp4HI GCNGC 2 cut(s) 381, 384
FspBI CTAG 1 cut(s) 326
GluI GCNGC 2 cut(s) 381, 384
HaeIII GGCC 3 cut(s) 12, 84, 599
HapII CCGG 2 cut(s) 9, 81
Hin1II CATG 5 cut(s) 190, 298, 334, 343, 689
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 4 cut(s) 5, 219, 306, 645
HpaII CCGG 2 cut(s) 9, 81
HphI GGTGA 2 cut(s) 266, 314
Hpy188I TCNGA 1 cut(s) 76
Hpy188III TCNNGA 3 cut(s) 99, 752, 771
Hpy99I CGWCG 2 cut(s) 139, 142
HpyAV CCTTC 4 cut(s) 23, 59, 75, 707
HpyCH4III ACNGT 2 cut(s) 169, 268
HpyCH4V TGCA 6 cut(s) 93, 383, 409, 657, 685, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 90, 389
Hsp92II CATG 5 cut(s) 190, 298, 334, 343, 689
Ksp22I TGATCA 2 cut(s) 153, 618
Kzo9I GATC 3 cut(s) 153, 310, 618
LguI GCTCTTC 1 cut(s) 32
Lsp1109I GCAGC 2 cut(s) 367, 395
LweI GCATC 2 cut(s) 265, 691
MaeI CTAG 1 cut(s) 326
MaeIII GTNAC 1 cut(s) 262
MalI GATC 3 cut(s) 155, 312, 620
MboI GATC 3 cut(s) 153, 310, 618
MboII GAAGA 3 cut(s) 19, 46, 358
MluCI AATT 3 cut(s) 94, 513, 531
MmeI TCCRAC 1 cut(s) 435
MnlI CCTC 8 cut(s) 42, 56, 82, 121, 155, 205, 296, 748
MseI TTAA 3 cut(s) 443, 561, 788
MspCI CTTAAG 1 cut(s) 787
MspI CCGG 2 cut(s) 9, 81
MspR9I CCNGG 3 cut(s) 81, 300, 596
Mva1269I GAATGC 2 cut(s) 181, 760
MvaI CCWGG 2 cut(s) 300, 596
MwoI GCNNNNNNNGC 2 cut(s) 90, 389
NciI CCSGG 1 cut(s) 81
NcoI CCATGG 1 cut(s) 330
NdeI CATATG 1 cut(s) 507
NdeII GATC 3 cut(s) 153, 310, 618
NlaIII CATG 5 cut(s) 190, 298, 334, 343, 689
NspI RCATGY 1 cut(s) 689
PaeI GCATGC 1 cut(s) 689
PaqCI CACCTGC 1 cut(s) 383
PciSI GCTCTTC 1 cut(s) 32
PctI GAATGC 2 cut(s) 181, 760
PfeI GAWTC 3 cut(s) 219, 306, 645
PflMI CCANNNNNTGG 2 cut(s) 187, 434
PkrI GCNGC 2 cut(s) 382, 385
Psp6I CCWGG 2 cut(s) 298, 594
PspGI CCWGG 2 cut(s) 298, 594
PspPI GGNCC 1 cut(s) 749
PstI CTGCAG 1 cut(s) 385
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 3 cut(s) 443, 561, 788
SatI GCNGC 2 cut(s) 381, 384
Sau3AI GATC 3 cut(s) 153, 310, 618
Sau96I GGNCC 1 cut(s) 749
ScrFI CCNGG 3 cut(s) 81, 300, 596
SfaNI GCATC 2 cut(s) 265, 691
SfcI CTRYAG 2 cut(s) 168, 381
SinI GGWCC 1 cut(s) 749
SmlI CTYRAG 1 cut(s) 787
SmoI CTYRAG 1 cut(s) 787
SphI GCATGC 1 cut(s) 689
Sse9I AATT 3 cut(s) 94, 513, 531
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 79, 298, 594
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 2 cut(s) 169, 268
TaqI TCGA 2 cut(s) 68, 309
TasI AATT 3 cut(s) 94, 513, 531
TfiI GAWTC 3 cut(s) 219, 306, 645
Tru1I TTAA 3 cut(s) 443, 561, 788
Tru9I TTAA 3 cut(s) 443, 561, 788
TscAI CASTG 2 cut(s) 376, 786
TseI GCWGC 2 cut(s) 380, 383
TspDTI ATGAA 6 cut(s) 5, 328, 480, 543, 726, 743
TspRI CASTG 2 cut(s) 376, 786
Van91I CCANNNNNTGG 2 cut(s) 187, 434
Vha464I CTTAAG 1 cut(s) 787
VpaK11BI GGWCC 1 cut(s) 749
XceI RCATGY 1 cut(s) 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 337
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.