Rw5G031400

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0004092
Physical Location & Seq
Reverse (-)
51513024 .. 51521706
8683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031400.1

Sequence Viewer

Length: 1338 bp
ATGGATCTAAAATGCAAAGTCTGTTTGACATTAATAGGAGGAATTGATCCCCATACTCACCTAGCCATGGAGTTCATGGGTACAGAAACTATTGATGATTTCTTCAGTGGTCAAGCTGCAGCGTTAGCAGGTGGAACAACTATGCACATTGACTTTGTTATACCAGTAAATGGAAGTTTAACGGCAGGGTTGGAAGCTTATGAAAAAAAATCTGGGAAGTCCTGTATGGATTATGGCTTCCATATGGCAATTACTAAATGGGATGAAATTGTTTCACAAGAAATGGAAATAATGGTTAAAGAGAAAGGTATAAACTCTTTCAAGTTTTTCATGGCCTACAAGGGGTCTCTTATGATCAACGATGAGCTTCTACTACAAGGATTCAAAAAGTGCAAGTCTCTTGGTGCTTTAGCTATGGTGCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAAAAGAATGAAAGAACTTGGTATTACAGGTCCAGAGGGGCATTCTCTTTCAAGACCCCCAGTGCTGGAAGGTGAGGCAACTGCTCGAGCCATTCGATTAGCAGCTTTTGTGAATACCCCCCTCTATGTAGTTCATGTGATGAGCATTGATGCCATGGAAGAAATTTCTAAAGCTCGCAAATCAGGGCTTAAGGTCATTGGCGAGCCAGTAGTCTCTGGGTTAGTGCTTAATGAATCTTGGCTTTGGGATCCTGACTTTATCACGGCTGCCAAGTATGTCATGAGTCCCCCTATCAGGGCTGCAGGACATGACAGAGCCCTCCAAGCTGCTCTTTCAATGGGAATTCTGCAGCTTGTAGGAACTGATCATTGTGCCTTCAATTCTACACAGAAAGCTCTTGGAATTGATGATTTCCAGAAAATTCCAAATGGTGTTAATGGTATTGAGGAGAGGATGCATGTCCTATGGGATACAATGGTGGAATCAGGTCAAATTTCTGTAACTGACTATGTCCGGTTGACAAGTGCTGAATGTGCTAGGATTTTCAATATATATCCGAGGAAGGGAGCAATTCGTGCTGGATCGGATGCCGATATAATTATATTAAATCCAAACTCAAGCTTTGAAATAAGTGCAAAGTCCCATCACTCTAAATCTGATACCAATGTCTATGAGGGATGGAGAGGGAAGGGAAAGGTTGAAGTCACTATTTCTGGTGGAAGAGTTGTTTGGCAAAATGATGAACTGAAGGTTGTTCCCGGTTCTGGCAAGTACATAGAAATGCCACCATTTAGTTATCTTTTTAATGGAATAGAGAAGGCCGATGCAAAGTACCTATCTTCTCTTAAGGCTCCCGTAAAGCGCTTAAGAACCATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

445

Amino Acids

48.74

Weight (kDa)

6.33

Isoelectric Point (pI)

30.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 11 - 395 1.4e-22 Amidohydrolase family
Amidohydro_3 PF07969 253 - 395 2.3e-07 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 119
Acc36I ACCTGC 1 cut(s) 119
AccB7I CCANNNNNTGG 1 cut(s) 170
AclWI GGATC 5 cut(s) 12, 41, 698, 711, 1045
AcsI RAATTY 4 cut(s) 618, 798, 876, 948
AcuI CTGAAG 2 cut(s) 88, 1223
AfaI GTAC 3 cut(s) 82, 1229, 1289
AfeI AGCGCT 1 cut(s) 1319
AfiI CCNNNNNNNGG 8 cut(s) 67, 170, 342, 520, 750, 751, 1019, 1220
AflII CTTAAG 3 cut(s) 644, 1301, 1321
AgsI TTSAA 8 cut(s) 322, 385, 507, 792, 835, 1003, 1082, 1157
AjuI GAANNNNNNNTTGG 2 cut(s) 1168, 1200
Alw26I GTCTC 3 cut(s) 351, 402, 673
AlwI GGATC 5 cut(s) 12, 41, 698, 711, 1045
Ama87I CYCGRG 1 cut(s) 540
Aor51HI AGCGCT 1 cut(s) 1319
AoxI GGCC 2 cut(s) 333, 1275
ApeKI GCWGC 7 cut(s) 116, 119, 557, 722, 755, 782, 805
ApoI RAATTY 4 cut(s) 618, 798, 876, 948
AseI ATTAAT 1 cut(s) 32
AspLEI GCGC 1 cut(s) 1320
AspS9I GGNCC 1 cut(s) 485
AsuC2I CCSGG 1 cut(s) 1215
AsuHPI GGTGA 2 cut(s) 50, 539
AvaI CYCGRG 1 cut(s) 540
AvaII GGWCC 1 cut(s) 485
BaeI ACNNNNGTAYC 4 cut(s) 918, 951, 1107, 1140
BamHI GGATCC 1 cut(s) 703
BanII GRGCYC 1 cut(s) 775
BbvI GCAGC 7 cut(s) 103, 131, 569, 709, 742, 769, 817
BccI CCATC 3 cut(s) 1107, 1128, 1337
BceAI ACGGC 2 cut(s) 198, 735
BciVI GTATCC 1 cut(s) 919
BclI TGATCA 2 cut(s) 354, 820
BcnI CCSGG 1 cut(s) 1215
BcoDI GTCTC 3 cut(s) 351, 402, 673
BfaI CTAG 2 cut(s) 62, 993
BfmI CTRYAG 3 cut(s) 117, 756, 803
BfoI RGCGCY 1 cut(s) 1321
BfrI CTTAAG 3 cut(s) 644, 1301, 1321
BfuAI ACCTGC 1 cut(s) 119
BfuI GTATCC 1 cut(s) 919
BisI GCNGC 7 cut(s) 117, 120, 558, 723, 756, 783, 806
BlsI GCNGC 7 cut(s) 118, 121, 559, 724, 757, 784, 807
Bme1390I CCNGG 1 cut(s) 1215
Bme18I GGWCC 1 cut(s) 485
BmeT110I CYCGRG 1 cut(s) 540
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 2 cut(s) 705, 1308
BmrFI CCNGG 1 cut(s) 1215
BmrI ACTGGG 1 cut(s) 509
BmsI GCATC 5 cut(s) 427, 595, 900, 1033, 1270
BmuI ACTGGG 1 cut(s) 509
BpuEI CTTGAG 1 cut(s) 1057
BpuMI CCSGG 1 cut(s) 1215
BsaI GGTCTC 1 cut(s) 351
BsaJI CCNNGG 3 cut(s) 66, 609, 1013
BsaWI WCCGGW 1 cut(s) 969
BsaXI ACNNNNNCTCC 2 cut(s) 426, 456
Bsc4I CCNNNNNNNGG 8 cut(s) 67, 170, 342, 520, 750, 751, 1019, 1220
Bse1I ACTGG 3 cut(s) 164, 515, 662
BseDI CCNNGG 3 cut(s) 66, 609, 1013
BseGI GGATG 4 cut(s) 268, 915, 1048, 1139
BseLI CCNNNNNNNGG 8 cut(s) 67, 170, 342, 520, 750, 751, 1019, 1220
BseNI ACTGG 3 cut(s) 164, 515, 662
BseRI GAGGAG 1 cut(s) 917
BseXI GCAGC 7 cut(s) 103, 131, 569, 709, 742, 769, 817
BshFI GGCC 2 cut(s) 335, 1277
BsiHKCI CYCGRG 1 cut(s) 540
BsiSI CCGG 2 cut(s) 970, 1215
BslFI GGGAC 2 cut(s) 726, 1081
BslI CCNNNNNNNGG 8 cut(s) 67, 170, 342, 520, 750, 751, 1019, 1220
BsmAI GTCTC 3 cut(s) 351, 402, 673
BsmFI GGGAC 2 cut(s) 726, 1081
BsmI GAATGC 1 cut(s) 496
BsnI GGCC 2 cut(s) 335, 1277
Bso31I GGTCTC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 540
Bsp1286I GDGCHC 1 cut(s) 775
Bsp143I GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
Bsp19I CCATGG 2 cut(s) 66, 609
BspANI GGCC 2 cut(s) 335, 1277
BspHI TCATGA 1 cut(s) 735
BspLI GGNNCC 2 cut(s) 705, 1308
BspMAI CTGCAG 3 cut(s) 121, 760, 807
BspMI ACCTGC 1 cut(s) 119
BspPI GGATC 5 cut(s) 12, 41, 698, 711, 1045
BspTI CTTAAG 3 cut(s) 644, 1301, 1321
BspTNI GGTCTC 1 cut(s) 351
BsrI ACTGG 3 cut(s) 164, 515, 662
BssECI CCNNGG 3 cut(s) 66, 609, 1013
BssMI GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
BssT1I CCWWGG 2 cut(s) 66, 609
Bst6I CTCTTC 1 cut(s) 1171
BstAFI CTTAAG 3 cut(s) 644, 1301, 1321
BstAPI GCANNNNNTGC 1 cut(s) 1031
BstC8I GCNNGC 3 cut(s) 423, 631, 659
BstDSI CCRYGG 2 cut(s) 66, 609
BstF5I GGATG 4 cut(s) 268, 915, 1048, 1139
BstH2I RGCGCY 1 cut(s) 1321
BstHHI GCGC 1 cut(s) 1320
BstKTI GATC 6 cut(s) 7, 49, 357, 706, 823, 1040
BstMAI GTCTC 3 cut(s) 351, 402, 673
BstMBI GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
BstMWI GCNNNNNNNGC 4 cut(s) 125, 779, 989, 1031
BstNSI RCATGY 2 cut(s) 425, 917
BstSCI CCNGG 1 cut(s) 1213
BstSFI CTRYAG 3 cut(s) 117, 756, 803
BstV1I GCAGC 7 cut(s) 103, 131, 569, 709, 742, 769, 817
BstX2I RGATCY 2 cut(s) 4, 703
BstYI RGATCY 2 cut(s) 4, 703
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 2 cut(s) 335, 1277
BtgI CCRYGG 2 cut(s) 66, 609
BtsCI GGATG 4 cut(s) 268, 915, 1048, 1139
BtsIMutI CAGTG 2 cut(s) 112, 522
BveI ACCTGC 1 cut(s) 119
Cac8I GCNNGC 3 cut(s) 423, 631, 659
CciI TCATGA 1 cut(s) 735
CfoI GCGC 1 cut(s) 1320
Cfr13I GGNCC 1 cut(s) 485
Csp6I GTAC 3 cut(s) 81, 1228, 1288
CviAII CATG 9 cut(s) 67, 76, 331, 422, 590, 610, 736, 764, 914
CviQI GTAC 3 cut(s) 81, 1228, 1288
DpnI GATC 6 cut(s) 6, 48, 356, 705, 822, 1039
DpnII GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
Eam1104I CTCTTC 1 cut(s) 1171
EarI CTCTTC 1 cut(s) 1171
Eco130I CCWWGG 2 cut(s) 66, 609
Eco24I GRGCYC 1 cut(s) 775
Eco31I GGTCTC 1 cut(s) 351
Eco47I GGWCC 1 cut(s) 485
Eco47III AGCGCT 1 cut(s) 1319
Eco57I CTGAAG 2 cut(s) 88, 1223
Eco88I CYCGRG 1 cut(s) 540
EcoRI GAATTC 1 cut(s) 798
EcoT14I CCWWGG 2 cut(s) 66, 609
EcoT22I ATGCAT 1 cut(s) 915
EcoT38I GRGCYC 1 cut(s) 775
ErhI CCWWGG 2 cut(s) 66, 609
FaeI CATG 9 cut(s) 70, 79, 334, 425, 593, 613, 739, 767, 917
FalI AAGNNNNNCTT 2 cut(s) 771, 803
FaqI GGGAC 2 cut(s) 726, 1081
FatI CATG 9 cut(s) 66, 75, 330, 421, 589, 609, 735, 763, 913
FauNDI CATATG 1 cut(s) 243
FbaI TGATCA 2 cut(s) 354, 820
Fnu4HI GCNGC 7 cut(s) 117, 120, 558, 723, 756, 783, 806
FokI GGATG 4 cut(s) 275, 922, 1055, 1146
FriOI GRGCYC 1 cut(s) 775
Fsp4HI GCNGC 7 cut(s) 117, 120, 558, 723, 756, 783, 806
FspBI CTAG 2 cut(s) 62, 993
GlaI GCGC 1 cut(s) 1319
GluI GCNGC 7 cut(s) 117, 120, 558, 723, 756, 783, 806
HaeII RGCGCY 1 cut(s) 1321
HaeIII GGCC 2 cut(s) 335, 1277
HapII CCGG 2 cut(s) 970, 1215
HhaI GCGC 1 cut(s) 1320
Hin1II CATG 9 cut(s) 70, 79, 334, 425, 593, 613, 739, 767, 917
Hin6I GCGC 1 cut(s) 1318
HinP1I GCGC 1 cut(s) 1318
HincII GTYRAC 1 cut(s) 975
HindII GTYRAC 1 cut(s) 975
HindIII AAGCTT 2 cut(s) 195, 1075
HinfI GANTC 4 cut(s) 381, 689, 739, 938
HpaII CCGG 2 cut(s) 970, 1215
HphI GGTGA 2 cut(s) 50, 539
Hpy166II GTNNAC 1 cut(s) 975
Hpy188I TCNGA 3 cut(s) 1014, 1042, 1114
Hpy188III TCNNGA 5 cut(s) 488, 507, 707, 736, 871
Hpy8I GTNNAC 1 cut(s) 975
HpyAV CCTTC 7 cut(s) 443, 518, 841, 1012, 1138, 1198, 1267
HpyF10VI GCNNNNNNNGC 4 cut(s) 125, 779, 989, 1031
Hsp92II CATG 9 cut(s) 70, 79, 334, 425, 593, 613, 739, 767, 917
HspAI GCGC 1 cut(s) 1318
Ksp22I TGATCA 2 cut(s) 354, 820
Kzo9I GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
LmnI GCTCC 2 cut(s) 1022, 1312
Lsp1109I GCAGC 7 cut(s) 103, 131, 569, 709, 742, 769, 817
LweI GCATC 5 cut(s) 427, 595, 900, 1033, 1270
MaeI CTAG 2 cut(s) 62, 993
MaeIII GTNAC 2 cut(s) 955, 1159
MalI GATC 6 cut(s) 6, 48, 356, 705, 822, 1039
MboI GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
MboII GAAGA 4 cut(s) 94, 626, 1188, 1287
MflI RGATCY 2 cut(s) 4, 703
MhlI GDGCHC 1 cut(s) 775
MlyI GAGTC 1 cut(s) 748
MmeI TCCRAC 1 cut(s) 171
Mph1103I ATGCAT 1 cut(s) 915
MslI CAYNNNNRTG 1 cut(s) 1235
MspCI CTTAAG 3 cut(s) 644, 1301, 1321
MspI CCGG 2 cut(s) 970, 1215
MspR9I CCNGG 1 cut(s) 1215
Mva1269I GAATGC 1 cut(s) 496
MwoI GCNNNNNNNGC 4 cut(s) 125, 779, 989, 1031
NciI CCSGG 1 cut(s) 1215
NcoI CCATGG 2 cut(s) 66, 609
NdeI CATATG 1 cut(s) 243
NdeII GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
NlaIII CATG 9 cut(s) 70, 79, 334, 425, 593, 613, 739, 767, 917
NlaIV GGNNCC 2 cut(s) 705, 1308
NmuCI GTSAC 1 cut(s) 1159
NsiI ATGCAT 1 cut(s) 915
NspI RCATGY 2 cut(s) 425, 917
PaeI GCATGC 1 cut(s) 425
PaeR7I CTCGAG 1 cut(s) 540
PagI TCATGA 1 cut(s) 735
PaqCI CACCTGC 1 cut(s) 119
PcsI WCGNNNNNNNCGW 1 cut(s) 547
PctI GAATGC 1 cut(s) 496
PfeI GAWTC 3 cut(s) 381, 689, 938
PflFI GACNNNGTC 1 cut(s) 965
PflMI CCANNNNNTGG 1 cut(s) 170
PkrI GCNGC 7 cut(s) 118, 121, 559, 724, 757, 784, 807
PleI GAGTC 1 cut(s) 747
PpsI GAGTC 1 cut(s) 747
PshBI ATTAAT 1 cut(s) 32
PspN4I GGNNCC 2 cut(s) 705, 1308
PspPI GGNCC 1 cut(s) 485
PspXI VCTCGAGB 1 cut(s) 540
PstI CTGCAG 3 cut(s) 121, 760, 807
PsuI RGATCY 2 cut(s) 4, 703
PsyI GACNNNGTC 1 cut(s) 965
RsaI GTAC 3 cut(s) 82, 1229, 1289
RsaNI GTAC 3 cut(s) 81, 1228, 1288
RseI CAYNNNNRTG 1 cut(s) 1235
SatI GCNGC 7 cut(s) 117, 120, 558, 723, 756, 783, 806
Sau3AI GATC 6 cut(s) 4, 46, 354, 703, 820, 1037
Sau96I GGNCC 1 cut(s) 485
SchI GAGTC 1 cut(s) 748
ScrFI CCNGG 1 cut(s) 1215
SduI GDGCHC 1 cut(s) 775
SfaNI GCATC 5 cut(s) 427, 595, 900, 1033, 1270
SfcI CTRYAG 3 cut(s) 117, 756, 803
Sfr274I CTCGAG 1 cut(s) 540
SinI GGWCC 1 cut(s) 485
SlaI CTCGAG 1 cut(s) 540
SmiMI CAYNNNNRTG 1 cut(s) 1235
SmlI CTYRAG 5 cut(s) 540, 644, 1072, 1301, 1321
SmoI CTYRAG 5 cut(s) 540, 644, 1072, 1301, 1321
SphI GCATGC 1 cut(s) 425
SspMI CTAG 2 cut(s) 62, 993
StyD4I CCNGG 1 cut(s) 1213
StyI CCWWGG 2 cut(s) 66, 609
TaqI TCGA 2 cut(s) 541, 550
TatI WGTACW 1 cut(s) 1227
TfiI GAWTC 3 cut(s) 381, 689, 938
TscAI CASTG 2 cut(s) 112, 522
TseFI GTSAC 1 cut(s) 1159
TseI GCWGC 7 cut(s) 116, 119, 557, 722, 755, 782, 805
Tsp45I GTSAC 1 cut(s) 1159
TspDTI ATGAA 9 cut(s) 64, 216, 279, 319, 462, 479, 578, 702, 1212
TspRI CASTG 2 cut(s) 112, 522
Tth111I GACNNNGTC 1 cut(s) 965
Van91I CCANNNNNTGG 1 cut(s) 170
Vha464I CTTAAG 3 cut(s) 644, 1301, 1321
VpaK11BI GGWCC 1 cut(s) 485
VspI ATTAAT 1 cut(s) 32
XapI RAATTY 4 cut(s) 618, 798, 876, 948
XceI RCATGY 2 cut(s) 425, 917
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XhoI CTCGAG 1 cut(s) 540
XspI CTAG 2 cut(s) 62, 993
Zsp2I ATGCAT 1 cut(s) 915
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.