Prupe.4G264100_v2.0.a1

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0004092
Physical Location & Seq
Forward (+)
19456496 .. 19465816
9321 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G264100.2

Sequence Viewer

Length: 1611 bp
ATGGCGTACAGCTCCAGTCCTCAACGCCTCCTCCATCTGTTATCTCTCATCCTTCTTCTCTTCGCCTTTTGTTCCATCTCAGAATCAAGCCAGTTTTGCGATGCTGGATTTGGATATGGCGAGTCGTGTGGCGTTTCTTCGTCTTCGTCGACCAAGTTGTTGATCAAAGGCGGAACTGTAGTGAATGCTCATGAGATGGAGGTCGCTGATGTTTATGTGGAGGATGGAATCATTGTTGCTGTTAGGCCTAATATTAAGGTTGGTGATGATGTCACTGTGCTTGAGGCCACTGGAAAGTTTGTCATGCCAGGAGGCATTGATCCTCATACCCACCTAGACATGGAGTTCATGGGTTCAGGAACCATTGATGATTTCTTCAGTGGTCAGGCTGCGGCGTTAGCTGGTGGAACAACAATGCACATTGACTTTGTTATACCAGTTAATGGAAGTTTATTGTCAGGATTAGAAGCTTATGAAAAAAAGTCTAAGAAGTCCTGCATGGATTATGGTTTCCATATGGCAATTACAAAATGGGATGAAGTTGTTTCAAAAGAAATGGAAATAATGGTCAATGAGAAAGGTATAAACTCTTTCAAGTTTTTCATGGCATACAAGGGGTCTTTTATGATCAATGATGAGCTTCTGCTACAAGCATTCAAAAAGTGCAAGTCTCTTGGTGCGTTAGCTATGGTTCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAGAAGAATGATAGAACTTGGTATTACTGGTCCAGAGGGACATGCTCTTTCAAGGCCCCCAGTGCTGGAAGGAGAAGCAACTGCTCGAGCAATTAAACTCGCAGGTTTTGTGAACACTCCCCTCTATGTAGTTCACGTGATGAGCATTGATGCTATGGAAGAAATTTCTAAAGCTCGTAAATCAGGGCTGAAGGTTATTGGAGAACCAGTAGTTTCTGGGTTAGTGCTGAATGATTCTTGGCTTTGGGATCCTGACTTTATCACTGCCGCTAAGTATGTCATGAGTCCCCCTATAAGGGCCGCAGGACATGGCAAAGCCCTTCAAGCTGCTCTTTCAATGGGAACTCTGCAGCTTGTAGGAACCGATCATTGTACCTTCAACTCAACACAGAAAGCTCTTGGAATTGATGATTTCCAGAAAATTCCAAATGGTGTCAATGGTATTGAGGAGAGGATGCATCTTGTATGGGATACAATGGTGGAATCCGGTCAAATTTCTGTTACTGACTATGTCCGGGTGACAAGTACCGAATGTGCTAGGATTTTCAATATATATCCGAGGAAGGGAGCAATTCGTGCCGGTTCGGATGCAGATATAATTATACTCAATCCGAACTCAAGCTTTGAAATAAGTGCAAAGTCCCACCACTCTAGATCTGATACCAATGTCTACGAGGGATGGAGAGGGAAGGGAAAGGTTGAAGTGACCATTTCTGGTGGAAGGGTTGTTTGGTTAAATGATGAGTTGAAGGTTTTTCCTGGTTCTGGCAAGTATGTAGAAATGCGACCTTTCAGTTATCTTTTCAATGGAATTGGCAAGGCAGATGCAAAGTATCTATCTTCTCTTAAAGCCCCTGTAAAGCGTTTCAGAGCTACCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

537

Amino Acids

58.27

Weight (kDa)

6.09

Isoelectric Point (pI)

31.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 821
AccB7I CCANNNNNTGG 1 cut(s) 443
AccI GTMKAC 2 cut(s) 149, 1398
AciI CCGC 4 cut(s) 171, 392, 996, 1029
AclWI GGATC 3 cut(s) 314, 971, 984
AcsI RAATTY 3 cut(s) 891, 1149, 1221
AcuI CTGAAG 2 cut(s) 361, 938
AcvI CACGTG 1 cut(s) 865
AfaI GTAC 3 cut(s) 8, 1102, 1255
AfiI CCNNNNNNNGG 7 cut(s) 340, 443, 793, 1023, 1024, 1292, 1493
AjnI CCWGG 2 cut(s) 307, 1486
AjuI GAANNNNNNNTTGG 2 cut(s) 1441, 1473
Alw26I GTCTC 1 cut(s) 675
AlwI GGATC 3 cut(s) 314, 971, 984
Ama87I CYCGRG 1 cut(s) 813
AoxI GGCC 4 cut(s) 245, 285, 782, 1026
ApeKI GCWGC 3 cut(s) 389, 1055, 1078
ApoI RAATTY 3 cut(s) 891, 1149, 1221
AspS9I GGNCC 3 cut(s) 758, 783, 1026
AsuC2I CCSGG 1 cut(s) 1244
AsuHPI GGTGA 2 cut(s) 275, 1258
AvaI CYCGRG 1 cut(s) 813
AvaII GGWCC 1 cut(s) 758
BaeI ACNNNNGTAYC 4 cut(s) 1191, 1224, 1380, 1413
BamHI GGATCC 1 cut(s) 976
BbrPI CACGTG 1 cut(s) 865
BbsI GAAGAC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 376, 1042, 1090
BccI CCATC 5 cut(s) 42, 83, 190, 218, 1401
BciT130I CCWGG 2 cut(s) 309, 1488
BciVI GTATCC 1 cut(s) 1192
BclI TGATCA 2 cut(s) 162, 627
BcnI CCSGG 1 cut(s) 1244
BcoDI GTCTC 1 cut(s) 675
BfaI CTAG 3 cut(s) 335, 1266, 1380
BfmI CTRYAG 2 cut(s) 177, 1076
BfuAI ACCTGC 1 cut(s) 821
BfuI GTATCC 1 cut(s) 1192
BglII AGATCT 1 cut(s) 1382
BisI GCNGC 6 cut(s) 390, 393, 996, 1029, 1056, 1079
BlsI GCNGC 6 cut(s) 391, 394, 997, 1030, 1057, 1080
Bme1390I CCNGG 3 cut(s) 309, 1244, 1488
Bme18I GGWCC 1 cut(s) 758
BmeT110I CYCGRG 1 cut(s) 813
BmgT120I GGNCC 3 cut(s) 758, 783, 1026
BmiI GGNNCC 4 cut(s) 361, 785, 978, 1090
BmrFI CCNGG 3 cut(s) 309, 1244, 1488
BmrI ACTGGG 1 cut(s) 782
BmsI GCATC 7 cut(s) 91, 700, 868, 1173, 1195, 1306, 1543
BmuI ACTGGG 1 cut(s) 782
BpiI GAAGAC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 302, 1330
BpuMI CCSGG 1 cut(s) 1244
BsaAI YACGTR 1 cut(s) 865
BsaJI CCNNGG 1 cut(s) 1286
BsaWI WCCGGW 1 cut(s) 1214
BsaXI ACNNNNNCTCC 6 cut(s) 15, 45, 699, 729, 921, 951
Bsc4I CCNNNNNNNGG 7 cut(s) 340, 443, 793, 1023, 1024, 1292, 1493
Bse118I RCCGGY 1 cut(s) 1307
Bse1I ACTGG 7 cut(s) 15, 91, 295, 437, 760, 788, 935
BseBI CCWGG 2 cut(s) 309, 1488
BseDI CCNNGG 1 cut(s) 1286
BseGI GGATG 6 cut(s) 48, 229, 541, 1188, 1321, 1412
BseLI CCNNNNNNNGG 7 cut(s) 340, 443, 793, 1023, 1024, 1292, 1493
BseMII CTCAG 1 cut(s) 93
BseNI ACTGG 7 cut(s) 15, 91, 295, 437, 760, 788, 935
BseRI GAGGAG 2 cut(s) 20, 1190
BseXI GCAGC 3 cut(s) 376, 1042, 1090
BshFI GGCC 4 cut(s) 247, 287, 784, 1028
BsiHKCI CYCGRG 1 cut(s) 813
BsiSI CCGG 3 cut(s) 1215, 1243, 1308
BslFI GGGAC 3 cut(s) 780, 999, 1354
BslI CCNNNNNNNGG 7 cut(s) 340, 443, 793, 1023, 1024, 1292, 1493
BsmAI GTCTC 1 cut(s) 675
BsmFI GGGAC 3 cut(s) 780, 999, 1354
BsmI GAATGC 2 cut(s) 190, 653
BsnI GGCC 4 cut(s) 247, 287, 784, 1028
BsoBI CYCGRG 1 cut(s) 813
Bsp143I GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
BspACI CCGC 4 cut(s) 171, 392, 996, 1029
BspANI GGCC 4 cut(s) 247, 287, 784, 1028
BspCNI CTCAG 1 cut(s) 92
BspHI TCATGA 2 cut(s) 190, 1008
BspLI GGNNCC 4 cut(s) 361, 785, 978, 1090
BspMAI CTGCAG 1 cut(s) 1080
BspMI ACCTGC 1 cut(s) 821
BspPI GGATC 3 cut(s) 314, 971, 984
BsrFI RCCGGY 1 cut(s) 1307
BsrI ACTGG 7 cut(s) 15, 91, 295, 437, 760, 788, 935
BssAI RCCGGY 1 cut(s) 1307
BssECI CCNNGG 1 cut(s) 1286
BssMI GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
Bst2UI CCWGG 2 cut(s) 309, 1488
Bst4CI ACNGT 2 cut(s) 178, 277
Bst6I CTCTTC 1 cut(s) 65
BstAPI GCANNNNNTGC 1 cut(s) 1304
BstBAI YACGTR 1 cut(s) 865
BstDEI CTNAG 3 cut(s) 79, 486, 999
BstF5I GGATG 6 cut(s) 48, 229, 541, 1188, 1321, 1412
BstKTI GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
BstMAI GTCTC 1 cut(s) 675
BstMBI GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
BstMWI GCNNNNNNNGC 5 cut(s) 96, 398, 790, 1052, 1304
BstNI CCWGG 2 cut(s) 309, 1488
BstNSI RCATGY 1 cut(s) 773
BstSCI CCNGG 3 cut(s) 307, 1242, 1486
BstSFI CTRYAG 2 cut(s) 177, 1076
BstV1I GCAGC 3 cut(s) 376, 1042, 1090
BstV2I GAAGAC 1 cut(s) 135
BstX2I RGATCY 2 cut(s) 976, 1382
BstYI RGATCY 2 cut(s) 976, 1382
BsuI GTATCC 1 cut(s) 1192
BsuRI GGCC 4 cut(s) 247, 287, 784, 1028
BtgZI GCGATG 1 cut(s) 114
BtsCI GGATG 6 cut(s) 48, 229, 541, 1188, 1321, 1412
BtsI GCAGTG 1 cut(s) 990
BtsIMutI CAGTG 5 cut(s) 273, 288, 385, 795, 990
BveI ACCTGC 1 cut(s) 821
CciI TCATGA 2 cut(s) 190, 1008
Cfr10I RCCGGY 1 cut(s) 1307
Cfr13I GGNCC 3 cut(s) 758, 783, 1026
Csp6I GTAC 3 cut(s) 7, 1101, 1254
CviQI GTAC 3 cut(s) 7, 1101, 1254
DdeI CTNAG 3 cut(s) 79, 486, 999
DpnI GATC 6 cut(s) 164, 321, 629, 978, 1095, 1384
DpnII GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 186
Eco147I AGGCCT 1 cut(s) 247
Eco47I GGWCC 1 cut(s) 758
Eco57I CTGAAG 2 cut(s) 361, 938
Eco72I CACGTG 1 cut(s) 865
Eco88I CYCGRG 1 cut(s) 813
EcoO109I RGGNCCY 1 cut(s) 783
EcoRII CCWGG 2 cut(s) 307, 1486
EcoT22I ATGCAT 1 cut(s) 1188
FalI AAGNNNNNCTT 2 cut(s) 1044, 1076
FaqI GGGAC 3 cut(s) 780, 999, 1354
FauNDI CATATG 1 cut(s) 516
FbaI TGATCA 2 cut(s) 162, 627
FblI GTMKAC 2 cut(s) 149, 1398
Fnu4HI GCNGC 6 cut(s) 390, 393, 996, 1029, 1056, 1079
FokI GGATG 6 cut(s) 35, 236, 548, 1195, 1328, 1419
Fsp4HI GCNGC 6 cut(s) 390, 393, 996, 1029, 1056, 1079
FspBI CTAG 3 cut(s) 335, 1266, 1380
GluI GCNGC 6 cut(s) 390, 393, 996, 1029, 1056, 1079
HaeIII GGCC 4 cut(s) 247, 287, 784, 1028
HapII CCGG 3 cut(s) 1215, 1243, 1308
HincII GTYRAC 1 cut(s) 150
HindII GTYRAC 1 cut(s) 150
HindIII AAGCTT 2 cut(s) 468, 1348
HinfI GANTC 6 cut(s) 83, 122, 228, 962, 1012, 1211
HpaII CCGG 3 cut(s) 1215, 1243, 1308
HphI GGTGA 2 cut(s) 275, 1258
Hpy166II GTNNAC 4 cut(s) 150, 841, 862, 1399
Hpy188I TCNGA 6 cut(s) 82, 1287, 1315, 1341, 1387, 1598
Hpy188III TCNNGA 8 cut(s) 191, 357, 459, 761, 980, 1009, 1144, 1380
Hpy8I GTNNAC 4 cut(s) 150, 841, 862, 1399
Hpy99I CGWCG 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 178, 277
HpyCH4IV ACGT 1 cut(s) 864
HpyCH4V TGCA 9 cut(s) 418, 498, 666, 698, 1078, 1186, 1319, 1364, 1556
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 398, 790, 1052, 1304
HpyF3I CTNAG 3 cut(s) 79, 486, 999
HpySE526I ACGT 1 cut(s) 864
Ksp22I TGATCA 2 cut(s) 162, 627
Kzo9I GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
LmnI GCTCC 2 cut(s) 17, 1295
Lsp1109I GCAGC 3 cut(s) 376, 1042, 1090
LweI GCATC 7 cut(s) 91, 700, 868, 1173, 1195, 1306, 1543
MaeI CTAG 3 cut(s) 335, 1266, 1380
MaeII ACGT 1 cut(s) 864
MaeIII GTNAC 4 cut(s) 271, 1228, 1246, 1432
MalI GATC 6 cut(s) 164, 321, 629, 978, 1095, 1384
MboI GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
MboII GAAGA 8 cut(s) 47, 52, 129, 135, 367, 744, 899, 1560
MflI RGATCY 2 cut(s) 976, 1382
MluCI AATT 9 cut(s) 522, 819, 891, 1131, 1149, 1221, 1299, 1326, 1539
MlyI GAGTC 2 cut(s) 131, 1021
Mph1103I ATGCAT 1 cut(s) 1188
MseI TTAA 5 cut(s) 255, 441, 822, 1463, 1575
MspI CCGG 3 cut(s) 1215, 1243, 1308
MspR9I CCNGG 3 cut(s) 309, 1244, 1488
Mva1269I GAATGC 2 cut(s) 190, 653
MvaI CCWGG 2 cut(s) 309, 1488
MwoI GCNNNNNNNGC 5 cut(s) 96, 398, 790, 1052, 1304
NciI CCSGG 1 cut(s) 1244
NdeI CATATG 1 cut(s) 516
NdeII GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
NlaIV GGNNCC 4 cut(s) 361, 785, 978, 1090
NmuCI GTSAC 3 cut(s) 271, 1246, 1432
NsiI ATGCAT 1 cut(s) 1188
NspI RCATGY 1 cut(s) 773
PaeR7I CTCGAG 1 cut(s) 813
PagI TCATGA 2 cut(s) 190, 1008
PceI AGGCCT 1 cut(s) 247
PcsI WCGNNNNNNNCGW 1 cut(s) 146
PctI GAATGC 2 cut(s) 190, 653
PfeI GAWTC 4 cut(s) 83, 228, 962, 1211
PflFI GACNNNGTC 1 cut(s) 1238
PflMI CCANNNNNTGG 1 cut(s) 443
PkrI GCNGC 6 cut(s) 391, 394, 997, 1030, 1057, 1080
PleI GAGTC 2 cut(s) 130, 1020
PmaCI CACGTG 1 cut(s) 865
PmlI CACGTG 1 cut(s) 865
PpsI GAGTC 2 cut(s) 130, 1020
Ppu21I YACGTR 1 cut(s) 865
Psp6I CCWGG 2 cut(s) 307, 1486
PspCI CACGTG 1 cut(s) 865
PspGI CCWGG 2 cut(s) 307, 1486
PspN4I GGNNCC 4 cut(s) 361, 785, 978, 1090
PspPI GGNCC 3 cut(s) 758, 783, 1026
PspXI VCTCGAGB 1 cut(s) 813
PstI CTGCAG 1 cut(s) 1080
PsuI RGATCY 2 cut(s) 976, 1382
PsyI GACNNNGTC 1 cut(s) 1238
RsaI GTAC 3 cut(s) 8, 1102, 1255
RsaNI GTAC 3 cut(s) 7, 1101, 1254
SalI GTCGAC 1 cut(s) 148
SaqAI TTAA 5 cut(s) 255, 441, 822, 1463, 1575
SatI GCNGC 6 cut(s) 390, 393, 996, 1029, 1056, 1079
Sau3AI GATC 6 cut(s) 162, 319, 627, 976, 1093, 1382
Sau96I GGNCC 3 cut(s) 758, 783, 1026
SchI GAGTC 2 cut(s) 131, 1021
ScrFI CCNGG 3 cut(s) 309, 1244, 1488
SfaNI GCATC 7 cut(s) 91, 700, 868, 1173, 1195, 1306, 1543
SfcI CTRYAG 2 cut(s) 177, 1076
Sfr274I CTCGAG 1 cut(s) 813
SinI GGWCC 1 cut(s) 758
SlaI CTCGAG 1 cut(s) 813
SmlI CTYRAG 3 cut(s) 281, 813, 1345
SmoI CTYRAG 3 cut(s) 281, 813, 1345
Sse9I AATT 9 cut(s) 522, 819, 891, 1131, 1149, 1221, 1299, 1326, 1539
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 4 cut(s) 171, 392, 996, 1029
SspI AATATT 1 cut(s) 253
SspMI CTAG 3 cut(s) 335, 1266, 1380
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 3 cut(s) 307, 1242, 1486
TaaI ACNGT 2 cut(s) 178, 277
TaiI ACGT 1 cut(s) 867
TaqI TCGA 2 cut(s) 149, 814
TasI AATT 9 cut(s) 522, 819, 891, 1131, 1149, 1221, 1299, 1326, 1539
TauI GCSGC 3 cut(s) 395, 998, 1031
TfiI GAWTC 4 cut(s) 83, 228, 962, 1211
Tru1I TTAA 5 cut(s) 255, 441, 822, 1463, 1575
Tru9I TTAA 5 cut(s) 255, 441, 822, 1463, 1575
TscAI CASTG 5 cut(s) 280, 295, 385, 795, 997
TseFI GTSAC 3 cut(s) 271, 1246, 1432
TseI GCWGC 3 cut(s) 389, 1055, 1078
Tsp45I GTSAC 3 cut(s) 271, 1246, 1432
TspDTI ATGAA 6 cut(s) 337, 489, 552, 592, 683, 735
TspRI CASTG 5 cut(s) 280, 295, 385, 795, 997
Tth111I GACNNNGTC 1 cut(s) 1238
Van91I CCANNNNNTGG 1 cut(s) 443
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 3 cut(s) 891, 1149, 1221
XbaI TCTAGA 1 cut(s) 1379
XceI RCATGY 1 cut(s) 773
XhoI CTCGAG 1 cut(s) 813
XmiI GTMKAC 2 cut(s) 149, 1398
XspI CTAG 3 cut(s) 335, 1266, 1380
Zsp2I ATGCAT 1 cut(s) 1188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.