Rh5DG355300

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0004092
Physical Location & Seq
Reverse (-)
52430993 .. 52439286
8294 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG355300.1

Sequence Viewer

Length: 1608 bp
ATGGACTCCGCTCTTCATCAGCTGCTCTTCCCTCTTTTATCTCTCCTCCTCCTACTCTTGGCCTTTCCTTCGACCTCCGAACCCGGCCAGTTCTGCAATTCTGGATTTGGATATGGCGATTTCGCCTGTGAGGTTTCGTCGTCGTCGGCCAAGTTGTTGATCAAAGGAGGGACTGTGGTGAATGCCCACCACATGGAGATTGCTGATGTTTATGTGGAGGATGGAATCATTGTTGCTGTCAGCCCCAATATCAAGGTTGGTGATGATGTTACTGTGCTTGATGCCAGTGGAAAGTTTGTCATGCCAGGAGGAATCGATCCCCATACTCACCTAGCCATGGAGTTCATGGGTACAGAAACTATTGATGATTTCTTCAGTGGTCAAGCTGCAGCGTTAGCAGGTGGAACAACTATGCACATTGACTTTGTTATACCAGTAAATGGAAGTTTAACGGCAGGGTTGGAAGCTTATGAAAAAAAATCTGGGAAGTCCTGTATGGATTATGGCTTCCATATGGCAATTACTAAATGGGATGAAATTGTTTCACAAGAAATGGAAATAATGGTTAAAGAGAAAGGTATAAACTCTTTCAAGTTTTTCCTGGCCTACAAGGGGTCTCTTATGATCAACGATGAGCTTCTACTACAAGGATTGAAAAAGTGCAAGTCTCTTGGTGCTTTAGCTATGGTGCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAAAAGAATGAAAGAACTTGGTATTACAGGTCCAGAGGGGCATTCTCTTTCAAGACCCCCAGTGCTGGAAGGTGAGGCAACTGCTCGAGCCATTCGATTAGCAGCTTTTGTGAATACCCCCCTCTATGTAGTTCATGTGATGAGCATTGATGCCATGGAAGAAATTTCTAAAGCTCGCAAATCAGGGCTTAAGGTCATTGGCGAGCCAGTAGTCTCTGGGTTAGTGCTTAATGAATCTTGGCTTTGGGATCCTGACTTTATCACGGCTGCCAAGTATGTCATGAGTCCCCCTATCAGGGCTGCAGGACATGACAGAGCCCTCCAAGCTGCTCTTTCAATGGGAATTCTGCAGCTTGTAGGAACTGATCATTGTGCCTTCAATTCTACACAGAAAGCTCTTGGAATTGATGATTTCCAGAAAATTCCAAATGGTGTTAATGGTATTGAGGAGAGGATGCATGTCCTATGGGATACAATGGTGGAATCAGGTCAAATTTCTGTAACTGACTATGTCCGGTTGACAAGTGCTGAATGTGCTAGGATTTTCAATATATATCCGAGGAAGGGAGCAATTCGTGCTGGATCGGATGCCGATATAATTATATTAAATCCAAACTCAAGCTTTGAAATAAGTGCAAAGTCCCATCACTCTAAATCTGATACCAATGTCTACGAGGGATGGAGAGGGAAGGGAAAGGTTGAAGTCACTATTTCTGGTGGAAGAGTTGTTTGGCAAAATGATGAACTGAAGGTTGTTCCTGGTTCTGGCAAGTACATAGAAATGCCACCATTCAGTTATCTTTTTAATGGAATAGAGAAGGCCGATGCAAAGTACCTATCTTCTCTGAAGGCTCCCGTAAAGCGCTTAAGAACCATCACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

535

Amino Acids

58.05

Weight (kDa)

5.71

Isoelectric Point (pI)

31.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 99 - 485 1.5e-24 Amidohydrolase family
Amidohydro_3 PF07969 343 - 485 3.3e-07 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
AccB7I CCANNNNNTGG 2 cut(s) 193, 440
AccBSI CCGCTC 1 cut(s) 11
AccI GTMKAC 1 cut(s) 1395
AciI CCGC 1 cut(s) 9
AclWI GGATC 4 cut(s) 311, 968, 981, 1315
AcoI YGGCCR 2 cut(s) 85, 147
AcsI RAATTY 4 cut(s) 888, 1068, 1146, 1218
AcuI CTGAAG 3 cut(s) 358, 1493, 1592
AfaI GTAC 3 cut(s) 352, 1499, 1559
AfeI AGCGCT 1 cut(s) 1589
AflII CTTAAG 2 cut(s) 914, 1591
AgsI TTSAA 8 cut(s) 592, 655, 777, 1062, 1105, 1273, 1352, 1427
AjnI CCWGG 3 cut(s) 304, 600, 1483
AjuI GAANNNNNNNTTGG 2 cut(s) 1438, 1470
Alw26I GTCTC 3 cut(s) 621, 672, 943
AlwI GGATC 4 cut(s) 311, 968, 981, 1315
Ama87I CYCGRG 1 cut(s) 810
Aor51HI AGCGCT 1 cut(s) 1589
AoxI GGCC 5 cut(s) 60, 85, 147, 603, 1545
ApeKI GCWGC 8 cut(s) 22, 386, 389, 827, 992, 1025, 1052, 1075
ApoI RAATTY 4 cut(s) 888, 1068, 1146, 1218
AspLEI GCGC 1 cut(s) 1590
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 190, 272, 320, 809
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 1 cut(s) 755
BaeI ACNNNNGTAYC 4 cut(s) 1188, 1221, 1377, 1410
BamHI GGATCC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 1045
BbvI GCAGC 8 cut(s) 9, 373, 401, 839, 979, 1012, 1039, 1087
BccI CCATC 4 cut(s) 215, 1377, 1398, 1607
BceAI ACGGC 2 cut(s) 468, 1005
BciT130I CCWGG 3 cut(s) 306, 602, 1485
BciVI GTATCC 1 cut(s) 1189
BclI TGATCA 3 cut(s) 159, 624, 1090
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 621, 672, 943
BfaI CTAG 2 cut(s) 332, 1263
BfmI CTRYAG 3 cut(s) 387, 1026, 1073
BfoI RGCGCY 1 cut(s) 1591
BfrI CTTAAG 2 cut(s) 914, 1591
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 8 cut(s) 23, 387, 390, 828, 993, 1026, 1053, 1076
BlsI GCNGC 8 cut(s) 24, 388, 391, 829, 994, 1027, 1054, 1077
Bme1390I CCNGG 4 cut(s) 84, 306, 602, 1485
Bme18I GGWCC 1 cut(s) 755
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 2 cut(s) 975, 1578
BmrFI CCNGG 4 cut(s) 84, 306, 602, 1485
BmrI ACTGGG 1 cut(s) 779
BmsI GCATC 6 cut(s) 271, 697, 865, 1170, 1303, 1540
BmuI ACTGGG 1 cut(s) 779
BpuEI CTTGAG 1 cut(s) 1327
BpuMI CCSGG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 3 cut(s) 336, 879, 1283
BsaWI WCCGGW 1 cut(s) 1239
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 696, 726
Bse1I ACTGG 5 cut(s) 88, 285, 434, 785, 932
BseBI CCWGG 3 cut(s) 306, 602, 1485
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 3 cut(s) 336, 879, 1283
BseGI GGATG 5 cut(s) 226, 538, 1185, 1318, 1409
BseNI ACTGG 5 cut(s) 88, 285, 434, 785, 932
BseRI GAGGAG 3 cut(s) 35, 38, 1187
BseXI GCAGC 8 cut(s) 9, 373, 401, 839, 979, 1012, 1039, 1087
BshFI GGCC 5 cut(s) 62, 87, 149, 605, 1547
BshVI ATCGAT 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 2 cut(s) 84, 1240
BslFI GGGAC 3 cut(s) 184, 996, 1351
BsmAI GTCTC 3 cut(s) 621, 672, 943
BsmFI GGGAC 3 cut(s) 184, 996, 1351
BsmI GAATGC 2 cut(s) 187, 766
BsnI GGCC 5 cut(s) 62, 87, 149, 605, 1547
Bso31I GGTCTC 1 cut(s) 621
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 1 cut(s) 1045
Bsp143I GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
Bsp19I CCATGG 2 cut(s) 336, 879
BspACI CCGC 1 cut(s) 9
BspANI GGCC 5 cut(s) 62, 87, 149, 605, 1547
BspDI ATCGAT 1 cut(s) 315
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 2 cut(s) 975, 1578
BspMAI CTGCAG 3 cut(s) 391, 1030, 1077
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 4 cut(s) 311, 968, 981, 1315
BspQI GCTCTTC 2 cut(s) 18, 32
BspTI CTTAAG 2 cut(s) 914, 1591
BspTNI GGTCTC 1 cut(s) 621
BsrBI CCGCTC 1 cut(s) 11
BsrI ACTGG 5 cut(s) 88, 285, 434, 785, 932
BssECI CCNNGG 3 cut(s) 336, 879, 1283
BssMI GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
BssT1I CCWWGG 2 cut(s) 336, 879
Bst2UI CCWGG 3 cut(s) 306, 602, 1485
Bst4CI ACNGT 2 cut(s) 175, 274
Bst6I CTCTTC 3 cut(s) 18, 32, 1441
BstAFI CTTAAG 2 cut(s) 914, 1591
BstAPI GCANNNNNTGC 1 cut(s) 1301
BstC8I GCNNGC 3 cut(s) 693, 901, 929
BstDSI CCRYGG 2 cut(s) 336, 879
BstF5I GGATG 5 cut(s) 226, 538, 1185, 1318, 1409
BstH2I RGCGCY 1 cut(s) 1591
BstHHI GCGC 1 cut(s) 1590
BstKTI GATC 6 cut(s) 162, 319, 627, 976, 1093, 1310
BstMAI GTCTC 3 cut(s) 621, 672, 943
BstMBI GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
BstMWI GCNNNNNNNGC 5 cut(s) 93, 395, 1049, 1259, 1301
BstNI CCWGG 3 cut(s) 306, 602, 1485
BstNSI RCATGY 2 cut(s) 695, 1187
BstSCI CCNGG 4 cut(s) 82, 304, 600, 1483
BstSFI CTRYAG 3 cut(s) 387, 1026, 1073
BstV1I GCAGC 8 cut(s) 9, 373, 401, 839, 979, 1012, 1039, 1087
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
Bsu15I ATCGAT 1 cut(s) 315
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 5 cut(s) 62, 87, 149, 605, 1547
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 2 cut(s) 336, 879
BtsCI GGATG 5 cut(s) 226, 538, 1185, 1318, 1409
BtsIMutI CAGTG 3 cut(s) 292, 382, 792
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 3 cut(s) 693, 901, 929
CciI TCATGA 1 cut(s) 1005
CfoI GCGC 1 cut(s) 1590
Cfr13I GGNCC 1 cut(s) 755
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 3 cut(s) 351, 1498, 1558
CviQI GTAC 3 cut(s) 351, 1498, 1558
DpnI GATC 6 cut(s) 161, 318, 626, 975, 1092, 1309
DpnII GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
EaeI YGGCCR 2 cut(s) 85, 147
Eam1104I CTCTTC 3 cut(s) 18, 32, 1441
EarI CTCTTC 3 cut(s) 18, 32, 1441
Eco130I CCWWGG 2 cut(s) 336, 879
Eco24I GRGCYC 1 cut(s) 1045
Eco31I GGTCTC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 755
Eco47III AGCGCT 1 cut(s) 1589
Eco57I CTGAAG 3 cut(s) 358, 1493, 1592
Eco88I CYCGRG 1 cut(s) 810
EcoRI GAATTC 1 cut(s) 1068
EcoRII CCWGG 3 cut(s) 304, 600, 1483
EcoT14I CCWWGG 2 cut(s) 336, 879
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 336, 879
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 3 cut(s) 184, 996, 1351
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 3 cut(s) 159, 624, 1090
FblI GTMKAC 1 cut(s) 1395
Fnu4HI GCNGC 8 cut(s) 23, 387, 390, 828, 993, 1026, 1053, 1076
FokI GGATG 5 cut(s) 233, 545, 1192, 1325, 1416
FriOI GRGCYC 1 cut(s) 1045
Fsp4HI GCNGC 8 cut(s) 23, 387, 390, 828, 993, 1026, 1053, 1076
FspBI CTAG 2 cut(s) 332, 1263
GlaI GCGC 1 cut(s) 1589
GluI GCNGC 8 cut(s) 23, 387, 390, 828, 993, 1026, 1053, 1076
HaeII RGCGCY 1 cut(s) 1591
HaeIII GGCC 5 cut(s) 62, 87, 149, 605, 1547
HapII CCGG 2 cut(s) 84, 1240
HhaI GCGC 1 cut(s) 1590
Hin6I GCGC 1 cut(s) 1588
HinP1I GCGC 1 cut(s) 1588
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 2 cut(s) 465, 1345
HinfI GANTC 6 cut(s) 5, 225, 312, 959, 1009, 1208
HpaII CCGG 2 cut(s) 84, 1240
HphI GGTGA 4 cut(s) 190, 272, 320, 809
Hpy166II GTNNAC 2 cut(s) 1245, 1396
Hpy188I TCNGA 5 cut(s) 79, 1284, 1312, 1384, 1572
Hpy188III TCNNGA 6 cut(s) 102, 758, 777, 977, 1006, 1141
Hpy8I GTNNAC 2 cut(s) 1245, 1396
Hpy99I CGWCG 3 cut(s) 142, 145, 148
HpyAV CCTTC 9 cut(s) 78, 713, 788, 1111, 1282, 1408, 1468, 1537, 1567
HpyCH4III ACNGT 2 cut(s) 175, 274
HpyF10VI GCNNNNNNNGC 5 cut(s) 93, 395, 1049, 1259, 1301
HspAI GCGC 1 cut(s) 1588
Ksp22I TGATCA 3 cut(s) 159, 624, 1090
Kzo9I GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
LguI GCTCTTC 2 cut(s) 18, 32
LmnI GCTCC 2 cut(s) 1292, 1582
Lsp1109I GCAGC 8 cut(s) 9, 373, 401, 839, 979, 1012, 1039, 1087
LweI GCATC 6 cut(s) 271, 697, 865, 1170, 1303, 1540
MaeI CTAG 2 cut(s) 332, 1263
MaeIII GTNAC 3 cut(s) 268, 1225, 1429
MalI GATC 6 cut(s) 161, 318, 626, 975, 1092, 1309
MbiI CCGCTC 1 cut(s) 11
MboI GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
MboII GAAGA 6 cut(s) 5, 19, 364, 896, 1458, 1557
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 1 cut(s) 1045
MlyI GAGTC 1 cut(s) 1018
MmeI TCCRAC 1 cut(s) 441
Mph1103I ATGCAT 1 cut(s) 1185
MseI TTAA 8 cut(s) 449, 567, 915, 954, 1161, 1331, 1530, 1592
MslI CAYNNNNRTG 1 cut(s) 1505
MspA1I CMGCKG 1 cut(s) 22
MspCI CTTAAG 2 cut(s) 914, 1591
MspI CCGG 2 cut(s) 84, 1240
MspR9I CCNGG 4 cut(s) 84, 306, 602, 1485
Mva1269I GAATGC 2 cut(s) 187, 766
MvaI CCWGG 3 cut(s) 306, 602, 1485
MwoI GCNNNNNNNGC 5 cut(s) 93, 395, 1049, 1259, 1301
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 2 cut(s) 336, 879
NdeI CATATG 1 cut(s) 513
NdeII GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
NlaIV GGNNCC 2 cut(s) 975, 1578
NmuCI GTSAC 1 cut(s) 1429
NsiI ATGCAT 1 cut(s) 1185
NspI RCATGY 2 cut(s) 695, 1187
PaeI GCATGC 1 cut(s) 695
PaeR7I CTCGAG 1 cut(s) 810
PagI TCATGA 1 cut(s) 1005
PaqCI CACCTGC 1 cut(s) 389
PciSI GCTCTTC 2 cut(s) 18, 32
PcsI WCGNNNNNNNCGW 1 cut(s) 817
PctI GAATGC 2 cut(s) 187, 766
PfeI GAWTC 4 cut(s) 225, 312, 959, 1208
PflFI GACNNNGTC 1 cut(s) 1235
PflMI CCANNNNNTGG 2 cut(s) 193, 440
PkrI GCNGC 8 cut(s) 24, 388, 391, 829, 994, 1027, 1054, 1077
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
Psp6I CCWGG 3 cut(s) 304, 600, 1483
PspGI CCWGG 3 cut(s) 304, 600, 1483
PspN4I GGNNCC 2 cut(s) 975, 1578
PspPI GGNCC 1 cut(s) 755
PspXI VCTCGAGB 1 cut(s) 810
PstI CTGCAG 3 cut(s) 391, 1030, 1077
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 1235
PvuII CAGCTG 1 cut(s) 22
RsaI GTAC 3 cut(s) 352, 1499, 1559
RsaNI GTAC 3 cut(s) 351, 1498, 1558
RseI CAYNNNNRTG 1 cut(s) 1505
SapI GCTCTTC 2 cut(s) 18, 32
SaqAI TTAA 8 cut(s) 449, 567, 915, 954, 1161, 1331, 1530, 1592
SatI GCNGC 8 cut(s) 23, 387, 390, 828, 993, 1026, 1053, 1076
Sau3AI GATC 6 cut(s) 159, 316, 624, 973, 1090, 1307
Sau96I GGNCC 1 cut(s) 755
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 4 cut(s) 84, 306, 602, 1485
SduI GDGCHC 1 cut(s) 1045
SfaNI GCATC 6 cut(s) 271, 697, 865, 1170, 1303, 1540
SfcI CTRYAG 3 cut(s) 387, 1026, 1073
Sfr274I CTCGAG 1 cut(s) 810
SinI GGWCC 1 cut(s) 755
SlaI CTCGAG 1 cut(s) 810
SmiMI CAYNNNNRTG 1 cut(s) 1505
SmlI CTYRAG 4 cut(s) 810, 914, 1342, 1591
SmoI CTYRAG 4 cut(s) 810, 914, 1342, 1591
SphI GCATGC 1 cut(s) 695
SsiI CCGC 1 cut(s) 9
SspMI CTAG 2 cut(s) 332, 1263
StyD4I CCNGG 4 cut(s) 82, 304, 600, 1483
StyI CCWWGG 2 cut(s) 336, 879
TaaI ACNGT 2 cut(s) 175, 274
TaqI TCGA 4 cut(s) 71, 315, 811, 820
TatI WGTACW 1 cut(s) 1497
TfiI GAWTC 4 cut(s) 225, 312, 959, 1208
Tru1I TTAA 8 cut(s) 449, 567, 915, 954, 1161, 1331, 1530, 1592
Tru9I TTAA 8 cut(s) 449, 567, 915, 954, 1161, 1331, 1530, 1592
TscAI CASTG 3 cut(s) 292, 382, 792
TseFI GTSAC 1 cut(s) 1429
TseI GCWGC 8 cut(s) 22, 386, 389, 827, 992, 1025, 1052, 1075
Tsp45I GTSAC 1 cut(s) 1429
TspDTI ATGAA 9 cut(s) 5, 334, 486, 549, 732, 749, 848, 972, 1482
TspRI CASTG 3 cut(s) 292, 382, 792
Tth111I GACNNNGTC 1 cut(s) 1235
Van91I CCANNNNNTGG 2 cut(s) 193, 440
Vha464I CTTAAG 2 cut(s) 914, 1591
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 4 cut(s) 888, 1068, 1146, 1218
XceI RCATGY 2 cut(s) 695, 1187
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 810
XmiI GTMKAC 1 cut(s) 1395
XspI CTAG 2 cut(s) 332, 1263
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.