Prupe.4G264000_v2.0.a1

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0004092
Physical Location & Seq
Forward (+)
19443220 .. 19454354
11135 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G264000.6

Sequence Viewer

Length: 1614 bp
ATGGCGTCCAGCTCCAGTCCTCAACGCCTTCTCCATCTGTTATCACTCATCCTTCTTCTCTTCGCCTTTTGTACCATCGCAGAATCAAGCCAGTTTTGCGATGCTGGAATTGGATATGGCGAGTCAACATGTGGCGTTTCTTCGTCTTCGTCGACCAAGTTGTTGATCAAAGGCGGAACTGTAGTGAATGCTCATGAGATGGAGGTCGCTGATGTTTATGTGGAGGATGGAATCATTGTTGCTGTTAGGCCTAATATTAAGGTTGGTGATGATGTCACTGTGCTTGATGCCACTGGAAAGTTTGTCATGCCAGGAGGCATTGATCCTCATACCCACCTAGACTTTGAGTTCATGGGTTCAGAAACCATTGATGATTTCTTCAGTGGTCAGGCTGCAGCGTTAGCTGGTGGAACAACAATGCACATTGACTTTGTTATACCAGTTAATGGAAGTTTATTGTCAGGATTAGAAGCTTATGAAAAAAAGTCTAAGAAGTCCTGCATGGATTATGGTTTCCATATGGCAATTACAAAATGGGATGAAGTTGTTTCAAAAGAAATGGAAATAATGGTCAATGAGAAAGGTATAAATTCTTTCAAGTTTTTCATGGCATACAAGGGGGCTCTTATGATCAATGATGAGCTTCTGCTACAAGGATTCAAAAAGTGCAAGTCTCTTGGTGCGTTAGCTATGGTTCATGCAGAAAATGGAGATGCTGCATATGAAGGACAGAGAAGAATGATAGAACTTGGTATTACTGGTCCAGAGGGACATGCTCTTTCAAGGCCCCCAGTGCTGGAAGGAGAAGCAACTGCTCGAGCAATTAAACTTGCAGGTTTCATGAACACTCCCCTCTATGTAGTTCACGTGATGAGCATTGATGCTATGGAAGAAATTTCTAAAGCTCGCAAATCAGGGCAGAAGGTTATTGGAGAACCAGTAGTTTCTGGGTTAGTGCTGAATGATTCTTGGGTTTGGGATCCTGACTTTATCACTGCCGCCAAGTATGTCATGAGTCCCCCTATAAGGGCCGCAAGACATGGCAAAGCCCTTCAAGCTGCTCTTTCAATGGGAATTCTGCAGCTTGTAGGAACCGATCATTGTCCTTTCAACTCAACGCAGAAAGCTCTTGGAATTGATGATTTCCGGAAAATTCCAAATGGTGTCAATGGTATTGAGGAGAGGATGCATCTTGTATGGGATACAATGGTGGAATCCGGTCAAATTTCTGTTACTGACTATGTCCGGGTGACAAGCACTGAATGTGCTAGGATTTTCAATATATATCCGAGGAAGGGAGCAATTCGTGCCGGTTCTGATGCAGATATAATTATACTCAATCCGAACTCAAGCTTTGAAATAAGTGCAAAGTCCCACCACTCTAGATCTGATACCAATGTCTTCGAGGGATGGAGAGGGAAGGGAAAGGTTGAAGTGACCATTTCTGGTGGAAGGGTTGTTTGGTTAAATGATGAGTTGAAGGTTTTTCCTGGTTCTGGCAAGTACGTAGAAATGCGACCCTTCAGTTATCTTTTCAATGGAATTGGCAAGGCAGATGCAAAGTATCTATCTTCTCTTAAAGCCCCTATAAAGCGTTTTAGAGCCACCACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

538

Amino Acids

58.4

Weight (kDa)

6.19

Isoelectric Point (pI)

31.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 446
AccI GTMKAC 1 cut(s) 152
AccIII TCCGGA 1 cut(s) 1146
AciI CCGC 3 cut(s) 174, 999, 1032
AclWI GGATC 3 cut(s) 317, 974, 987
AcsI RAATTY 5 cut(s) 589, 894, 1074, 1152, 1224
AcuI CTGAAG 2 cut(s) 364, 1507
AcvI CACGTG 1 cut(s) 868
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 73, 1505
AfiI CCNNNNNNNGG 6 cut(s) 446, 796, 1026, 1027, 1295, 1496
AflIII ACRYGT 1 cut(s) 128
AjnI CCWGG 2 cut(s) 310, 1489
AjuI GAANNNNNNNTTGG 2 cut(s) 1444, 1476
Alw26I GTCTC 1 cut(s) 678
AlwI GGATC 3 cut(s) 317, 974, 987
Ama87I CYCGRG 1 cut(s) 816
Aor13HI TCCGGA 1 cut(s) 1146
AoxI GGCC 3 cut(s) 248, 785, 1029
ApeKI GCWGC 5 cut(s) 392, 395, 716, 1058, 1081
ApoI RAATTY 5 cut(s) 589, 894, 1074, 1152, 1224
AspS9I GGNCC 3 cut(s) 761, 786, 1029
AsuC2I CCSGG 1 cut(s) 1247
AsuHPI GGTGA 2 cut(s) 278, 1261
AvaI CYCGRG 1 cut(s) 816
AvaII GGWCC 1 cut(s) 761
BaeI ACNNNNGTAYC 4 cut(s) 1194, 1227, 1383, 1416
BamHI GGATCC 1 cut(s) 979
BanII GRGCYC 1 cut(s) 625
BbrPI CACGTG 1 cut(s) 868
BbsI GAAGAC 2 cut(s) 138, 1393
BbvI GCAGC 5 cut(s) 379, 407, 703, 1045, 1093
BccI CCATC 5 cut(s) 42, 83, 193, 221, 1404
BciT130I CCWGG 2 cut(s) 312, 1491
BciVI GTATCC 1 cut(s) 1195
BclI TGATCA 2 cut(s) 165, 630
BcnI CCSGG 1 cut(s) 1247
BcoDI GTCTC 1 cut(s) 678
BfaI CTAG 3 cut(s) 338, 1269, 1383
BfmI CTRYAG 3 cut(s) 180, 393, 1079
BfuAI ACCTGC 1 cut(s) 824
BfuI GTATCC 1 cut(s) 1195
BglII AGATCT 1 cut(s) 1385
BisI GCNGC 7 cut(s) 393, 396, 717, 999, 1032, 1059, 1082
BlsI GCNGC 7 cut(s) 394, 397, 718, 1000, 1033, 1060, 1083
Bme1390I CCNGG 3 cut(s) 312, 1247, 1491
Bme18I GGWCC 1 cut(s) 761
BmeT110I CYCGRG 1 cut(s) 816
BmgT120I GGNCC 3 cut(s) 761, 786, 1029
BmiI GGNNCC 3 cut(s) 788, 981, 1093
BmrFI CCNGG 3 cut(s) 312, 1247, 1491
BmrI ACTGGG 1 cut(s) 785
BmsI GCATC 8 cut(s) 91, 277, 703, 871, 1176, 1198, 1309, 1546
BmuI ACTGGG 1 cut(s) 785
BpiI GAAGAC 2 cut(s) 138, 1393
BpuEI CTTGAG 1 cut(s) 1333
BpuMI CCSGG 1 cut(s) 1247
BsaAI YACGTR 2 cut(s) 868, 1507
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 1289
BsaWI WCCGGW 2 cut(s) 1146, 1217
BsaXI ACNNNNNCTCC 4 cut(s) 15, 45, 924, 954
Bsc4I CCNNNNNNNGG 6 cut(s) 446, 796, 1026, 1027, 1295, 1496
Bse118I RCCGGY 1 cut(s) 1310
Bse1I ACTGG 7 cut(s) 15, 91, 298, 440, 763, 791, 938
BseAI TCCGGA 1 cut(s) 1146
BseBI CCWGG 2 cut(s) 312, 1491
BseDI CCNNGG 1 cut(s) 1289
BseGI GGATG 5 cut(s) 48, 232, 544, 1191, 1415
BseLI CCNNNNNNNGG 6 cut(s) 446, 796, 1026, 1027, 1295, 1496
BseNI ACTGG 7 cut(s) 15, 91, 298, 440, 763, 791, 938
BseRI GAGGAG 1 cut(s) 1193
BseXI GCAGC 5 cut(s) 379, 407, 703, 1045, 1093
BshFI GGCC 3 cut(s) 250, 787, 1031
BsiHKCI CYCGRG 1 cut(s) 816
BsiSI CCGG 4 cut(s) 1147, 1218, 1246, 1311
BslFI GGGAC 3 cut(s) 783, 1002, 1357
BslI CCNNNNNNNGG 6 cut(s) 446, 796, 1026, 1027, 1295, 1496
BsmAI GTCTC 1 cut(s) 678
BsmFI GGGAC 3 cut(s) 783, 1002, 1357
BsmI GAATGC 1 cut(s) 193
BsnI GGCC 3 cut(s) 250, 787, 1031
BsoBI CYCGRG 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 625
Bsp13I TCCGGA 1 cut(s) 1146
Bsp143I GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
BspACI CCGC 3 cut(s) 174, 999, 1032
BspANI GGCC 3 cut(s) 250, 787, 1031
BspEI TCCGGA 1 cut(s) 1146
BspHI TCATGA 3 cut(s) 193, 840, 1011
BspLI GGNNCC 3 cut(s) 788, 981, 1093
BspMAI CTGCAG 2 cut(s) 397, 1083
BspMI ACCTGC 1 cut(s) 824
BspPI GGATC 3 cut(s) 317, 974, 987
BsrFI RCCGGY 1 cut(s) 1310
BsrI ACTGG 7 cut(s) 15, 91, 298, 440, 763, 791, 938
BssAI RCCGGY 1 cut(s) 1310
BssECI CCNNGG 1 cut(s) 1289
BssMI GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 312, 1491
Bst4CI ACNGT 2 cut(s) 181, 280
Bst6I CTCTTC 1 cut(s) 65
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1307
BstBAI YACGTR 2 cut(s) 868, 1507
BstC8I GCNNGC 1 cut(s) 907
BstDEI CTNAG 1 cut(s) 489
BstF5I GGATG 5 cut(s) 48, 232, 544, 1191, 1415
BstKTI GATC 6 cut(s) 168, 325, 633, 982, 1099, 1388
BstMAI GTCTC 1 cut(s) 678
BstMBI GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
BstMWI GCNNNNNNNGC 5 cut(s) 96, 401, 793, 1055, 1307
BstNI CCWGG 2 cut(s) 312, 1491
BstNSI RCATGY 2 cut(s) 132, 776
BstSCI CCNGG 3 cut(s) 310, 1245, 1489
BstSFI CTRYAG 3 cut(s) 180, 393, 1079
BstSNI TACGTA 1 cut(s) 1507
BstV1I GCAGC 5 cut(s) 379, 407, 703, 1045, 1093
BstV2I GAAGAC 2 cut(s) 138, 1393
BstX2I RGATCY 2 cut(s) 979, 1385
BstYI RGATCY 2 cut(s) 979, 1385
BsuI GTATCC 1 cut(s) 1195
BsuRI GGCC 3 cut(s) 250, 787, 1031
BtgZI GCGATG 2 cut(s) 61, 114
BtsCI GGATG 5 cut(s) 48, 232, 544, 1191, 1415
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 6 cut(s) 276, 291, 388, 798, 993, 1257
BveI ACCTGC 1 cut(s) 824
Cac8I GCNNGC 1 cut(s) 907
CciI TCATGA 3 cut(s) 193, 840, 1011
Cfr10I RCCGGY 1 cut(s) 1310
Cfr13I GGNCC 3 cut(s) 761, 786, 1029
Csp6I GTAC 2 cut(s) 72, 1504
CviQI GTAC 2 cut(s) 72, 1504
DdeI CTNAG 1 cut(s) 489
DpnI GATC 6 cut(s) 167, 324, 632, 981, 1098, 1387
DpnII GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
EciI GGCGGA 1 cut(s) 189
Eco105I TACGTA 1 cut(s) 1507
Eco147I AGGCCT 1 cut(s) 250
Eco24I GRGCYC 1 cut(s) 625
Eco47I GGWCC 1 cut(s) 761
Eco57I CTGAAG 2 cut(s) 364, 1507
Eco72I CACGTG 1 cut(s) 868
Eco88I CYCGRG 1 cut(s) 816
EcoO109I RGGNCCY 1 cut(s) 786
EcoRI GAATTC 1 cut(s) 1074
EcoRII CCWGG 2 cut(s) 310, 1489
EcoT22I ATGCAT 1 cut(s) 1191
EcoT38I GRGCYC 1 cut(s) 625
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 3 cut(s) 783, 1002, 1357
FauNDI CATATG 2 cut(s) 519, 721
FbaI TGATCA 2 cut(s) 165, 630
FblI GTMKAC 1 cut(s) 152
Fnu4HI GCNGC 7 cut(s) 393, 396, 717, 999, 1032, 1059, 1082
FokI GGATG 5 cut(s) 35, 239, 551, 1198, 1422
FriOI GRGCYC 1 cut(s) 625
Fsp4HI GCNGC 7 cut(s) 393, 396, 717, 999, 1032, 1059, 1082
FspBI CTAG 3 cut(s) 338, 1269, 1383
GluI GCNGC 7 cut(s) 393, 396, 717, 999, 1032, 1059, 1082
HaeIII GGCC 3 cut(s) 250, 787, 1031
HapII CCGG 4 cut(s) 1147, 1218, 1246, 1311
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 2 cut(s) 126, 153
HindII GTYRAC 2 cut(s) 126, 153
HindIII AAGCTT 2 cut(s) 471, 1351
HinfI GANTC 7 cut(s) 83, 122, 231, 657, 965, 1015, 1214
HpaII CCGG 4 cut(s) 1147, 1218, 1246, 1311
HphI GGTGA 2 cut(s) 278, 1261
Hpy166II GTNNAC 3 cut(s) 126, 153, 865
Hpy188I TCNGA 5 cut(s) 361, 1290, 1318, 1344, 1390
Hpy188III TCNNGA 8 cut(s) 194, 462, 764, 841, 983, 1012, 1147, 1383
Hpy8I GTNNAC 3 cut(s) 126, 153, 865
Hpy99I CGWCG 1 cut(s) 154
HpyCH4III ACNGT 2 cut(s) 181, 280
HpyCH4IV ACGT 2 cut(s) 867, 1506
HpyF10VI GCNNNNNNNGC 5 cut(s) 96, 401, 793, 1055, 1307
HpyF3I CTNAG 1 cut(s) 489
HpySE526I ACGT 2 cut(s) 867, 1506
Hsp92I GRCGYC 1 cut(s) 5
Kpn2I TCCGGA 1 cut(s) 1146
Ksp22I TGATCA 2 cut(s) 165, 630
Kzo9I GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
LmnI GCTCC 2 cut(s) 17, 1298
Lsp1109I GCAGC 5 cut(s) 379, 407, 703, 1045, 1093
LweI GCATC 8 cut(s) 91, 277, 703, 871, 1176, 1198, 1309, 1546
MaeI CTAG 3 cut(s) 338, 1269, 1383
MaeII ACGT 2 cut(s) 867, 1506
MaeIII GTNAC 4 cut(s) 274, 1231, 1249, 1435
MalI GATC 6 cut(s) 167, 324, 632, 981, 1098, 1387
MboI GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
MboII GAAGA 9 cut(s) 47, 52, 132, 138, 370, 747, 902, 1393, 1563
MflI RGATCY 2 cut(s) 979, 1385
MhlI GDGCHC 1 cut(s) 625
MlyI GAGTC 2 cut(s) 131, 1024
Mph1103I ATGCAT 1 cut(s) 1191
MroI TCCGGA 1 cut(s) 1146
MseI TTAA 6 cut(s) 258, 444, 825, 1466, 1578, 1612
MspI CCGG 4 cut(s) 1147, 1218, 1246, 1311
MspR9I CCNGG 3 cut(s) 312, 1247, 1491
Mva1269I GAATGC 1 cut(s) 193
MvaI CCWGG 2 cut(s) 312, 1491
MwoI GCNNNNNNNGC 5 cut(s) 96, 401, 793, 1055, 1307
NciI CCSGG 1 cut(s) 1247
NdeI CATATG 2 cut(s) 519, 721
NdeII GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
NlaIV GGNNCC 3 cut(s) 788, 981, 1093
NmuCI GTSAC 3 cut(s) 274, 1249, 1435
NsiI ATGCAT 1 cut(s) 1191
NspI RCATGY 2 cut(s) 132, 776
PaeR7I CTCGAG 1 cut(s) 816
PagI TCATGA 3 cut(s) 193, 840, 1011
PceI AGGCCT 1 cut(s) 250
PciI ACATGT 1 cut(s) 128
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PctI GAATGC 1 cut(s) 193
PfeI GAWTC 5 cut(s) 83, 231, 657, 965, 1214
PflFI GACNNNGTC 1 cut(s) 1241
PflMI CCANNNNNTGG 1 cut(s) 446
PkrI GCNGC 7 cut(s) 394, 397, 718, 1000, 1033, 1060, 1083
PleI GAGTC 2 cut(s) 130, 1023
PmaCI CACGTG 1 cut(s) 868
PmlI CACGTG 1 cut(s) 868
PpsI GAGTC 2 cut(s) 130, 1023
Ppu21I YACGTR 2 cut(s) 868, 1507
PscI ACATGT 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 310, 1489
PspCI CACGTG 1 cut(s) 868
PspGI CCWGG 2 cut(s) 310, 1489
PspN4I GGNNCC 3 cut(s) 788, 981, 1093
PspPI GGNCC 3 cut(s) 761, 786, 1029
PspXI VCTCGAGB 1 cut(s) 816
PstI CTGCAG 2 cut(s) 397, 1083
PsuI RGATCY 2 cut(s) 979, 1385
PsyI GACNNNGTC 1 cut(s) 1241
RsaI GTAC 2 cut(s) 73, 1505
RsaNI GTAC 2 cut(s) 72, 1504
SalI GTCGAC 1 cut(s) 151
SaqAI TTAA 6 cut(s) 258, 444, 825, 1466, 1578, 1612
SatI GCNGC 7 cut(s) 393, 396, 717, 999, 1032, 1059, 1082
Sau3AI GATC 6 cut(s) 165, 322, 630, 979, 1096, 1385
Sau96I GGNCC 3 cut(s) 761, 786, 1029
SchI GAGTC 2 cut(s) 131, 1024
ScrFI CCNGG 3 cut(s) 312, 1247, 1491
SduI GDGCHC 1 cut(s) 625
SfaNI GCATC 8 cut(s) 91, 277, 703, 871, 1176, 1198, 1309, 1546
SfcI CTRYAG 3 cut(s) 180, 393, 1079
Sfr274I CTCGAG 1 cut(s) 816
SinI GGWCC 1 cut(s) 761
SlaI CTCGAG 1 cut(s) 816
SmlI CTYRAG 2 cut(s) 816, 1348
SmoI CTYRAG 2 cut(s) 816, 1348
SnaBI TACGTA 1 cut(s) 1507
SseBI AGGCCT 1 cut(s) 250
SsiI CCGC 3 cut(s) 174, 999, 1032
SspI AATATT 1 cut(s) 256
SspMI CTAG 3 cut(s) 338, 1269, 1383
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 3 cut(s) 310, 1245, 1489
TaaI ACNGT 2 cut(s) 181, 280
TaiI ACGT 2 cut(s) 870, 1509
TaqI TCGA 3 cut(s) 152, 817, 1404
TauI GCSGC 2 cut(s) 1001, 1034
TfiI GAWTC 5 cut(s) 83, 231, 657, 965, 1214
Tru1I TTAA 6 cut(s) 258, 444, 825, 1466, 1578, 1612
Tru9I TTAA 6 cut(s) 258, 444, 825, 1466, 1578, 1612
TscAI CASTG 6 cut(s) 283, 298, 388, 798, 1000, 1264
TseFI GTSAC 3 cut(s) 274, 1249, 1435
TseI GCWGC 5 cut(s) 392, 395, 716, 1058, 1081
Tsp45I GTSAC 3 cut(s) 274, 1249, 1435
TspDTI ATGAA 8 cut(s) 340, 492, 555, 595, 686, 738, 829, 857
TspRI CASTG 6 cut(s) 283, 298, 388, 798, 1000, 1264
Tth111I GACNNNGTC 1 cut(s) 1241
Van91I CCANNNNNTGG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 761
XapI RAATTY 5 cut(s) 589, 894, 1074, 1152, 1224
XbaI TCTAGA 1 cut(s) 1382
XceI RCATGY 2 cut(s) 132, 776
XhoI CTCGAG 1 cut(s) 816
XmiI GTMKAC 1 cut(s) 152
XspI CTAG 3 cut(s) 338, 1269, 1383
Zsp2I ATGCAT 1 cut(s) 1191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.