Rh5AG332200

Amidohydrolase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0004092
Physical Location & Seq
Reverse (-)
53012663 .. 53021252
8590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG332200.1

Sequence Viewer

Length: 1296 bp
ATGGACTCCGCTCTTCATCAACTGCTCTTCCCTCTTTTATCTCTCCTCCTCCTTCTCTTCGCCTTTCCTTCGACCTCCGAACCCGGCCAGTTCTGCAATTCTGGATTTGGATATGGCGATTTCGCCTGTGAGGTTTCGTCGTCGTCGTCCAAGTTGTTGATCAAAGGAGGGACTGTGGTGAATGCCCACCACATGGAGATTGCTGATGTTTATGTGGAGGATGGAATCATTGTTGCTGTCAGCCCCAATATCAAGGTTGGTGATGATGTCACTGTGCTTGATGCCAGTGGAAAGTTTGTCATGCCAGGAGGAATCGATCCCCATACTCACCTAGCCATGGAGTTCATGGGTACAGAAACTATTGATGATTTCTTCAGTGGTCAAGCTGCAGCGTTAGCAGGTGGAACAACTATGCACATTGACTTTGTTATACCAGTAAATGGAAGTTTAACGGCAGGGTTGGAAGCTTATGAAAAAAAATCTGGGAAGTCCTGTATGGATTATGGCTTCCATATGGCAATTACTAAATGGGATGAAATTGTTTCACAAGAAATGGAAATAATGGTTAAAGAGAAAGGTATAAACTCTTTCAAGTTTTTCATGGCCTACAAGGGGTCTCTTATGATCAACGATGAGCTTCTACTACAAGGATTCAAAAAGTGCAAGTCTCTTGGTGCTTTAGCTATGGTGCATGCAGAAAATGGAGATGCTGTATATGAAGGACAGAAAAGAATGAAAGAACTTGGTATTACAGGTCCAGAGGGGCATTCTCTTTCAAGACCCCCAGTGCTGGAAGGTGAGGCAACTGCTCGAGCCATTCGATTAGCAGCTTTTGTGAATACCCCCCTCTATGTAGTTCATGTGATGAGCATTGATGCCATGGAAGAAATTTCTAAAGCTCGCAAATCAGGGGTTAAGGTCATTGGCGAGCCAGTAGTCTCTGGGTTAGTGCTTAATGAATCTTGGCTTTGGGATCCTGACTTTATCACTGCTGCCAAGTATGTCATGAGTCCCCCTATCAGGGCTGCAGGACATGACAGAGCCCTCCAAGCTGCTCTTTCAATGGGAATTCTGCAGCTTGTAGGAACTGATCATTGTGCCTTCAATTCTACACAGAAAGCTCTTGGAATTGATGATTTCCAGAAAATTCCAAATGGTGTTAATGGTATTGAGGAGAGGATGCATGTCCTATGGGATACAATGGTGGAATCAGGTCAAATTTCTGTAACTGACTATGTCCGGTTGACAAGTGCTGAATGGTATCTTATCTTTTCTTTGCTTATATCTGAAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100
Pfam Domains
Protein Families
Amidohydro_1

Protein Analysis

431

Amino Acids

46.76

Weight (kDa)

5.02

Isoelectric Point (pI)

31.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidohydro_1 PF01979 99 - 417 7.8e-13 Amidohydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 389
Acc36I ACCTGC 1 cut(s) 389
AccB7I CCANNNNNTGG 2 cut(s) 193, 440
AccBSI CCGCTC 1 cut(s) 11
AciI CCGC 1 cut(s) 9
AclWI GGATC 3 cut(s) 311, 968, 981
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 4 cut(s) 888, 1068, 1146, 1218
AcuI CTGAAG 1 cut(s) 358
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 7 cut(s) 193, 337, 440, 612, 790, 1020, 1021
AgsI TTSAA 5 cut(s) 592, 655, 777, 1062, 1105
AjnI CCWGG 1 cut(s) 304
AluBI AGCT 9 cut(s) 386, 467, 637, 683, 830, 899, 1052, 1078, 1121
AluI AGCT 9 cut(s) 386, 467, 637, 683, 830, 899, 1052, 1078, 1121
Alw26I GTCTC 3 cut(s) 621, 672, 943
AlwI GGATC 3 cut(s) 311, 968, 981
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 2 cut(s) 85, 603
ApeKI GCWGC 7 cut(s) 386, 389, 827, 992, 1025, 1052, 1075
ApoI RAATTY 4 cut(s) 888, 1068, 1146, 1218
AspS9I GGNCC 1 cut(s) 755
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 190, 272, 320, 809
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 1 cut(s) 755
BaeI ACNNNNGTAYC 2 cut(s) 1188, 1221
BamHI GGATCC 1 cut(s) 973
BanII GRGCYC 1 cut(s) 1045
BbvI GCAGC 7 cut(s) 373, 401, 839, 979, 1012, 1039, 1087
BccI CCATC 1 cut(s) 215
BceAI ACGGC 1 cut(s) 468
BciT130I CCWGG 1 cut(s) 306
BciVI GTATCC 1 cut(s) 1189
BclI TGATCA 3 cut(s) 159, 624, 1090
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 3 cut(s) 621, 672, 943
BfaI CTAG 1 cut(s) 332
BfmI CTRYAG 3 cut(s) 387, 1026, 1073
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 1189
BisI GCNGC 7 cut(s) 387, 390, 828, 993, 1026, 1053, 1076
BlsI GCNGC 7 cut(s) 388, 391, 829, 994, 1027, 1054, 1077
Bme1390I CCNGG 2 cut(s) 84, 306
Bme18I GGWCC 1 cut(s) 755
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 1 cut(s) 755
BmiI GGNNCC 1 cut(s) 975
BmrFI CCNGG 2 cut(s) 84, 306
BmrI ACTGGG 1 cut(s) 779
BmsI GCATC 4 cut(s) 271, 697, 865, 1170
BmuI ACTGGG 1 cut(s) 779
BpuMI CCSGG 1 cut(s) 84
Bsa29I ATCGAT 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 621
BsaJI CCNNGG 2 cut(s) 336, 879
BsaWI WCCGGW 1 cut(s) 1239
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 696, 726
Bsc4I CCNNNNNNNGG 7 cut(s) 193, 337, 440, 612, 790, 1020, 1021
Bse1I ACTGG 5 cut(s) 88, 285, 434, 785, 932
BseBI CCWGG 1 cut(s) 306
BseCI ATCGAT 1 cut(s) 315
BseDI CCNNGG 2 cut(s) 336, 879
BseGI GGATG 3 cut(s) 226, 538, 1185
BseLI CCNNNNNNNGG 7 cut(s) 193, 337, 440, 612, 790, 1020, 1021
BseNI ACTGG 5 cut(s) 88, 285, 434, 785, 932
BseRI GAGGAG 3 cut(s) 35, 38, 1187
BseXI GCAGC 7 cut(s) 373, 401, 839, 979, 1012, 1039, 1087
BshFI GGCC 2 cut(s) 87, 605
BshVI ATCGAT 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 2 cut(s) 84, 1240
BslFI GGGAC 2 cut(s) 184, 996
BslI CCNNNNNNNGG 7 cut(s) 193, 337, 440, 612, 790, 1020, 1021
BsmAI GTCTC 3 cut(s) 621, 672, 943
BsmFI GGGAC 2 cut(s) 184, 996
BsmI GAATGC 2 cut(s) 187, 766
BsnI GGCC 2 cut(s) 87, 605
Bso31I GGTCTC 1 cut(s) 621
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 1 cut(s) 1045
Bsp143I GATC 5 cut(s) 159, 316, 624, 973, 1090
Bsp19I CCATGG 2 cut(s) 336, 879
BspACI CCGC 1 cut(s) 9
BspANI GGCC 2 cut(s) 87, 605
BspDI ATCGAT 1 cut(s) 315
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 1 cut(s) 975
BspMAI CTGCAG 3 cut(s) 391, 1030, 1077
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 3 cut(s) 311, 968, 981
BspQI GCTCTTC 2 cut(s) 18, 32
BspTNI GGTCTC 1 cut(s) 621
BsrBI CCGCTC 1 cut(s) 11
BsrI ACTGG 5 cut(s) 88, 285, 434, 785, 932
BssECI CCNNGG 2 cut(s) 336, 879
BssMI GATC 5 cut(s) 159, 316, 624, 973, 1090
BssT1I CCWWGG 2 cut(s) 336, 879
Bst2UI CCWGG 1 cut(s) 306
Bst4CI ACNGT 2 cut(s) 175, 274
Bst6I CTCTTC 3 cut(s) 18, 32, 62
BstC8I GCNNGC 3 cut(s) 693, 901, 929
BstDSI CCRYGG 2 cut(s) 336, 879
BstF5I GGATG 3 cut(s) 226, 538, 1185
BstKTI GATC 5 cut(s) 162, 319, 627, 976, 1093
BstMAI GTCTC 3 cut(s) 621, 672, 943
BstMBI GATC 5 cut(s) 159, 316, 624, 973, 1090
BstMWI GCNNNNNNNGC 3 cut(s) 93, 395, 1049
BstNI CCWGG 1 cut(s) 306
BstNSI RCATGY 2 cut(s) 695, 1187
BstSCI CCNGG 2 cut(s) 82, 304
BstSFI CTRYAG 3 cut(s) 387, 1026, 1073
BstV1I GCAGC 7 cut(s) 373, 401, 839, 979, 1012, 1039, 1087
BstX2I RGATCY 1 cut(s) 973
BstYI RGATCY 1 cut(s) 973
Bsu15I ATCGAT 1 cut(s) 315
BsuI GTATCC 1 cut(s) 1189
BsuRI GGCC 2 cut(s) 87, 605
BsuTUI ATCGAT 1 cut(s) 315
BtgI CCRYGG 2 cut(s) 336, 879
BtsCI GGATG 3 cut(s) 226, 538, 1185
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 5 cut(s) 270, 292, 382, 792, 987
BveI ACCTGC 1 cut(s) 389
Cac8I GCNNGC 3 cut(s) 693, 901, 929
CciI TCATGA 1 cut(s) 1005
Cfr13I GGNCC 1 cut(s) 755
ClaI ATCGAT 1 cut(s) 315
Csp6I GTAC 1 cut(s) 351
CviQI GTAC 1 cut(s) 351
DpnI GATC 5 cut(s) 161, 318, 626, 975, 1092
DpnII GATC 5 cut(s) 159, 316, 624, 973, 1090
EaeI YGGCCR 1 cut(s) 85
Eam1104I CTCTTC 3 cut(s) 18, 32, 62
EarI CTCTTC 3 cut(s) 18, 32, 62
Eco130I CCWWGG 2 cut(s) 336, 879
Eco24I GRGCYC 1 cut(s) 1045
Eco31I GGTCTC 1 cut(s) 621
Eco47I GGWCC 1 cut(s) 755
Eco57I CTGAAG 1 cut(s) 358
Eco88I CYCGRG 1 cut(s) 810
EcoRI GAATTC 1 cut(s) 1068
EcoRII CCWGG 1 cut(s) 304
EcoT14I CCWWGG 2 cut(s) 336, 879
EcoT22I ATGCAT 1 cut(s) 1185
EcoT38I GRGCYC 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 336, 879
FalI AAGNNNNNCTT 2 cut(s) 1041, 1073
FaqI GGGAC 2 cut(s) 184, 996
FauNDI CATATG 1 cut(s) 513
FbaI TGATCA 3 cut(s) 159, 624, 1090
Fnu4HI GCNGC 7 cut(s) 387, 390, 828, 993, 1026, 1053, 1076
FokI GGATG 3 cut(s) 233, 545, 1192
FriOI GRGCYC 1 cut(s) 1045
Fsp4HI GCNGC 7 cut(s) 387, 390, 828, 993, 1026, 1053, 1076
FspBI CTAG 1 cut(s) 332
GluI GCNGC 7 cut(s) 387, 390, 828, 993, 1026, 1053, 1076
HaeIII GGCC 2 cut(s) 87, 605
HapII CCGG 2 cut(s) 84, 1240
HincII GTYRAC 1 cut(s) 1245
HindII GTYRAC 1 cut(s) 1245
HindIII AAGCTT 1 cut(s) 465
HinfI GANTC 7 cut(s) 5, 225, 312, 651, 959, 1009, 1208
HpaII CCGG 2 cut(s) 84, 1240
HphI GGTGA 4 cut(s) 190, 272, 320, 809
Hpy166II GTNNAC 1 cut(s) 1245
Hpy188I TCNGA 2 cut(s) 79, 1288
Hpy188III TCNNGA 6 cut(s) 102, 758, 777, 977, 1006, 1141
Hpy8I GTNNAC 1 cut(s) 1245
Hpy99I CGWCG 3 cut(s) 142, 145, 148
HpyAV CCTTC 5 cut(s) 62, 78, 713, 788, 1111
HpyCH4III ACNGT 2 cut(s) 175, 274
HpyCH4V TGCA 9 cut(s) 96, 389, 415, 663, 691, 695, 1028, 1075, 1183
HpyF10VI GCNNNNNNNGC 3 cut(s) 93, 395, 1049
Ksp22I TGATCA 3 cut(s) 159, 624, 1090
Kzo9I GATC 5 cut(s) 159, 316, 624, 973, 1090
LguI GCTCTTC 2 cut(s) 18, 32
Lsp1109I GCAGC 7 cut(s) 373, 401, 839, 979, 1012, 1039, 1087
LweI GCATC 4 cut(s) 271, 697, 865, 1170
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 2 cut(s) 268, 1225
MalI GATC 5 cut(s) 161, 318, 626, 975, 1092
MbiI CCGCTC 1 cut(s) 11
MboI GATC 5 cut(s) 159, 316, 624, 973, 1090
MboII GAAGA 5 cut(s) 5, 19, 49, 364, 896
MflI RGATCY 1 cut(s) 973
MhlI GDGCHC 1 cut(s) 1045
MluCI AATT 9 cut(s) 97, 519, 537, 888, 1068, 1105, 1128, 1146, 1218
MlyI GAGTC 1 cut(s) 1018
MmeI TCCRAC 1 cut(s) 441
Mph1103I ATGCAT 1 cut(s) 1185
MseI TTAA 5 cut(s) 449, 567, 915, 954, 1161
MspI CCGG 2 cut(s) 84, 1240
MspR9I CCNGG 2 cut(s) 84, 306
Mva1269I GAATGC 2 cut(s) 187, 766
MvaI CCWGG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 93, 395, 1049
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 2 cut(s) 336, 879
NdeI CATATG 1 cut(s) 513
NdeII GATC 5 cut(s) 159, 316, 624, 973, 1090
NlaIV GGNNCC 1 cut(s) 975
NmuCI GTSAC 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 1185
NspI RCATGY 2 cut(s) 695, 1187
PaeI GCATGC 1 cut(s) 695
PaeR7I CTCGAG 1 cut(s) 810
PagI TCATGA 1 cut(s) 1005
PaqCI CACCTGC 1 cut(s) 389
PciSI GCTCTTC 2 cut(s) 18, 32
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 817
PctI GAATGC 2 cut(s) 187, 766
PfeI GAWTC 5 cut(s) 225, 312, 651, 959, 1208
PflFI GACNNNGTC 1 cut(s) 1235
PflMI CCANNNNNTGG 2 cut(s) 193, 440
PkrI GCNGC 7 cut(s) 388, 391, 829, 994, 1027, 1054, 1077
PleI GAGTC 1 cut(s) 1017
PpsI GAGTC 1 cut(s) 1017
Psp6I CCWGG 1 cut(s) 304
PspGI CCWGG 1 cut(s) 304
PspN4I GGNNCC 1 cut(s) 975
PspPI GGNCC 1 cut(s) 755
PspXI VCTCGAGB 1 cut(s) 810
PstI CTGCAG 3 cut(s) 391, 1030, 1077
PsuI RGATCY 1 cut(s) 973
PsyI GACNNNGTC 1 cut(s) 1235
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SapI GCTCTTC 2 cut(s) 18, 32
SaqAI TTAA 5 cut(s) 449, 567, 915, 954, 1161
SatI GCNGC 7 cut(s) 387, 390, 828, 993, 1026, 1053, 1076
Sau3AI GATC 5 cut(s) 159, 316, 624, 973, 1090
Sau96I GGNCC 1 cut(s) 755
SchI GAGTC 1 cut(s) 1018
ScrFI CCNGG 2 cut(s) 84, 306
SduI GDGCHC 1 cut(s) 1045
SfaNI GCATC 4 cut(s) 271, 697, 865, 1170
SfcI CTRYAG 3 cut(s) 387, 1026, 1073
Sfr274I CTCGAG 1 cut(s) 810
SinI GGWCC 1 cut(s) 755
SlaI CTCGAG 1 cut(s) 810
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
SphI GCATGC 1 cut(s) 695
Sse9I AATT 9 cut(s) 97, 519, 537, 888, 1068, 1105, 1128, 1146, 1218
SsiI CCGC 1 cut(s) 9
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 82, 304
StyI CCWWGG 2 cut(s) 336, 879
TaaI ACNGT 2 cut(s) 175, 274
TaqI TCGA 4 cut(s) 71, 315, 811, 820
TasI AATT 9 cut(s) 97, 519, 537, 888, 1068, 1105, 1128, 1146, 1218
TfiI GAWTC 5 cut(s) 225, 312, 651, 959, 1208
Tru1I TTAA 5 cut(s) 449, 567, 915, 954, 1161
Tru9I TTAA 5 cut(s) 449, 567, 915, 954, 1161
TscAI CASTG 5 cut(s) 277, 292, 382, 792, 994
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 7 cut(s) 386, 389, 827, 992, 1025, 1052, 1075
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 9 cut(s) 5, 334, 486, 549, 589, 732, 749, 848, 972
TspRI CASTG 5 cut(s) 277, 292, 382, 792, 994
Tth111I GACNNNGTC 1 cut(s) 1235
Van91I CCANNNNNTGG 2 cut(s) 193, 440
VpaK11BI GGWCC 1 cut(s) 755
XapI RAATTY 4 cut(s) 888, 1068, 1146, 1218
XceI RCATGY 2 cut(s) 695, 1187
XcmI CCANNNNNNNNNTGG 1 cut(s) 343
XhoI CTCGAG 1 cut(s) 810
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 1 cut(s) 1185
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.