FvH4_3g28620

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017520
Physical Location & Seq
Reverse (-)
21618806 .. 21622608
3803 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g28620.t1

Sequence Viewer

Length: 1569 bp
ATGGCCAGCAGAGGTCCAAGAATCCAAGCTCTTGACCTCCCATGGCTACTACAACTTATTTTCCTCTTTTCAACCTTCATGATCAGTTCTTGTTATTCTCCTCCCTTGAGAAAAATACCGAGGCTCAGCATCCTACAAAGACCTATTACTTTTCGAAACCACAGCAACTCTGTTGCTGTCCGCCACCGTTTCCGGACATACTTTTATGCTCAAACACTAGACCACTTCAGCTACGAGCCAGCGAGTTATACCACCTTTCTGCAGCGTTACATGGTAAGCTCTATGCATTGGAGAGGTGCCCTTGCAGCCGCTCCTATATTCGTCTACCTTGGTGGGGAGGAGTCCTTGGAAGAAAGTATCGGTTCCATCGGATTTCTCACCGACAATGCGCAAAAATTTGGTGCTCTAATGGTATATTTAGAGCATCGGTACTATGGGAAATCAATTCCTTCTAGAGATAACATGCAAGATATATACAATACTACCAGTCTAGGATACCTGAATTCCGGCCAAGCTCTAGCCGATTATGCAGATATTATACTACACTTGAAGAGGAATTTATCCGCCGAGGCTTCTCCAGTAATTGTCATTGGAGGCTCTTATGGTGGAATGCTTGCAGCATGGTTTCGCCTCAAATACCCGCACATTGCTATGGGGGCATTGGCCTCTTCCGCCCCGCTTCTTTACTTCGATGACATTACACCATCAGATGGGTTTCATTCTGTGGTCTCAGACAACTTCAGGGAAGCGAGTGAGAGTTGCTATAATACCATAAAGAAATCATGGTCTGAGATCGATAGAATGGTTCTGGACCAAGACAATGGTCTTGACATGCTCACTGAGAAATTTCGAACTTGCGAACCTTTGGAGGATGGAGATGAACTCGAGCTAAAGACCTACTTGGAGAACCTATATATCACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAGTCAGATCTGCAACAGTATTGAAGAAGCAGCAGCAAGAGGAGATGATACCATTGATCGAATCGCCTCGGCTGTTAATAATTTAACTCGTCCAGGAGAGAATAGTGGTCGATGCTTGGAACTAAGTAGCGAACTTCGCCCATCAGATGATACAGTTAATGGTTTGAACTGGTTATGGCAGGCATGTACTGAACTACTAATGCCAATTGGTCGAGAAACAAACGAGACTATGTTCCAGGCAGAACCATTTGATGTCGATTCCTACAGTCGAATGTGCCGCCAGCTATATCAAGGTTCCCGCCTGAGACCCAATTGGATCACAACCTATTATGGAGGGCATCATATAAGGATGACCTTGGAGAAGTTTGCCAGTAACATCATCTTCTCAAATGGCCGCCGTGATCCTTTTAGTGCTGCCGGGGTATTGACAAATATATCGCCCAGCGTAGTAGCCGTGACAACATCTCAAGGTTCTCATTGCTTGGACATATTGCCTGCAAGGATAAACGATCCCAGATGGTTAGTTCTGCAGCGGAACATAGAACTTGGGATCATCGCAGGATGGATTCGCAACTACTACAACGATTTGCGTTTCTATTCTACTGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

523

Amino Acids

59.19

Weight (kDa)

5.55

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 71 - 498 1.2e-83 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 390
AccB1I GGYRCC 1 cut(s) 296
AccB7I CCANNNNNTGG 1 cut(s) 710
AccBSI CCGCTC 1 cut(s) 311
AccI GTMKAC 1 cut(s) 324
AccIII TCCGGA 1 cut(s) 192
AclWI GGATC 4 cut(s) 1280, 1352, 1460, 1514
AcoI YGGCCR 3 cut(s) 3, 508, 1348
AcsI RAATTY 4 cut(s) 395, 502, 556, 845
AcuI CTGAAG 2 cut(s) 211, 724
AfaI GTAC 2 cut(s) 431, 1144
AfiI CCNNNNNNNGG 2 cut(s) 334, 710
AgsI TTSAA 4 cut(s) 72, 550, 980, 1123
AjnI CCWGG 2 cut(s) 1048, 1191
AluBI AGCT 6 cut(s) 29, 231, 279, 515, 889, 1240
AluI AGCT 6 cut(s) 29, 231, 279, 515, 889, 1240
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 3 cut(s) 733, 1175, 1255
AlwI GGATC 4 cut(s) 1280, 1352, 1460, 1514
Ama87I CYCGRG 1 cut(s) 884
Aor13HI TCCGGA 1 cut(s) 192
AoxI GGCC 4 cut(s) 3, 508, 663, 1348
ApeKI GCWGC 7 cut(s) 262, 305, 617, 986, 989, 1370, 1486
ApoI RAATTY 4 cut(s) 395, 502, 556, 845
AspLEI GCGC 1 cut(s) 391
AspS9I GGNCC 2 cut(s) 14, 811
AsuC2I CCSGG 1 cut(s) 1375
AsuHPI GGTGA 1 cut(s) 370
AsuII TTCGAA 2 cut(s) 154, 850
AvaI CYCGRG 1 cut(s) 884
AvaII GGWCC 2 cut(s) 14, 811
BaeGI GKGCMC 1 cut(s) 301
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 296
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 7 cut(s) 274, 317, 629, 998, 1001, 1357, 1498
BccI CCATC 7 cut(s) 374, 704, 712, 866, 1105, 1467, 1512
BceAI ACGGC 2 cut(s) 1338, 1394
BciT130I CCWGG 2 cut(s) 1050, 1193
BciVI GTATCC 1 cut(s) 488
BclI TGATCA 1 cut(s) 81
BcnI CCSGG 1 cut(s) 1375
BcoDI GTCTC 3 cut(s) 733, 1175, 1255
BfaI CTAG 4 cut(s) 218, 453, 491, 518
BfmI CTRYAG 3 cut(s) 260, 1219, 1484
BfuI GTATCC 1 cut(s) 488
BglII AGATCT 1 cut(s) 963
BlpI GCTNAGC 1 cut(s) 125
Bme1390I CCNGG 3 cut(s) 1050, 1193, 1375
Bme18I GGWCC 2 cut(s) 14, 811
BmeT110I CYCGRG 1 cut(s) 884
BmgT120I GGNCC 2 cut(s) 14, 811
BmiI GGNNCC 3 cut(s) 298, 364, 1252
BmrFI CCNGG 3 cut(s) 1050, 1193, 1375
BmsI GCATC 5 cut(s) 138, 433, 932, 1058, 1303
BoxI GACNNNNGTC 1 cut(s) 822
BplI GAGNNNNNCTC 2 cut(s) 867, 899
BpmI CTGGAG 1 cut(s) 561
Bpu1102I GCTNAGC 1 cut(s) 125
Bpu14I TTCGAA 2 cut(s) 154, 850
BpuEI CTTGAG 2 cut(s) 127, 1407
BpuMI CCSGG 1 cut(s) 1375
Bsa29I ATCGAT 1 cut(s) 795
BsaI GGTCTC 2 cut(s) 733, 1255
BsaJI CCNNGG 8 cut(s) 41, 119, 328, 345, 567, 1023, 1311, 1374
BsaWI WCCGGW 1 cut(s) 192
Bsc4I CCNNNNNNNGG 2 cut(s) 334, 710
Bse1I ACTGG 5 cut(s) 486, 578, 953, 1130, 1326
Bse3DI GCAATG 2 cut(s) 645, 1432
BseAI TCCGGA 1 cut(s) 192
BseBI CCWGG 2 cut(s) 1050, 1193
BseCI ATCGAT 1 cut(s) 795
BseDI CCNNGG 8 cut(s) 41, 119, 328, 345, 567, 1023, 1311, 1374
BseGI GGATG 4 cut(s) 129, 877, 1311, 1523
BseLI CCNNNNNNNGG 2 cut(s) 334, 710
BseMI GCAATG 2 cut(s) 645, 1432
BseMII CTCAG 6 cut(s) 139, 744, 780, 831, 941, 1250
BseNI ACTGG 5 cut(s) 486, 578, 953, 1130, 1326
BseRI GAGGAG 3 cut(s) 90, 353, 1011
BseSI GKGCMC 1 cut(s) 301
BseXI GCAGC 7 cut(s) 274, 317, 629, 998, 1001, 1357, 1498
BseYI CCCAGC 1 cut(s) 1397
BshFI GGCC 4 cut(s) 5, 510, 665, 1350
BshNI GGYRCC 1 cut(s) 296
BshVI ATCGAT 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 406
BsiHKCI CYCGRG 1 cut(s) 884
BsiSI CCGG 3 cut(s) 193, 507, 1374
BslI CCNNNNNNNGG 2 cut(s) 334, 710
BsmAI GTCTC 3 cut(s) 733, 1175, 1255
BsmI GAATGC 1 cut(s) 615
BsnI GGCC 4 cut(s) 5, 510, 665, 1350
Bso31I GGTCTC 2 cut(s) 733, 1255
BsoBI CYCGRG 1 cut(s) 884
Bsp119I TTCGAA 2 cut(s) 154, 850
Bsp1286I GDGCHC 2 cut(s) 301, 406
Bsp13I TCCGGA 1 cut(s) 192
Bsp143I GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
Bsp1720I GCTNAGC 1 cut(s) 125
Bsp19I CCATGG 1 cut(s) 41
BspANI GGCC 4 cut(s) 5, 510, 665, 1350
BspCNI CTCAG 6 cut(s) 138, 743, 781, 832, 940, 1251
BspDI ATCGAT 1 cut(s) 795
BspEI TCCGGA 1 cut(s) 192
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 3 cut(s) 298, 364, 1252
BspMAI CTGCAG 2 cut(s) 264, 1488
BspPI GGATC 4 cut(s) 1280, 1352, 1460, 1514
BspT104I TTCGAA 2 cut(s) 154, 850
BspT107I GGYRCC 1 cut(s) 296
BspTNI GGTCTC 2 cut(s) 733, 1255
BsrBI CCGCTC 1 cut(s) 311
BsrDI GCAATG 2 cut(s) 645, 1432
BsrI ACTGG 5 cut(s) 486, 578, 953, 1130, 1326
BssECI CCNNGG 8 cut(s) 41, 119, 328, 345, 567, 1023, 1311, 1374
BssMI GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
BssT1I CCWWGG 4 cut(s) 41, 328, 345, 1311
Bst2UI CCWGG 2 cut(s) 1050, 1193
Bst4CI ACNGT 4 cut(s) 188, 974, 1111, 1223
Bst6I CTCTTC 2 cut(s) 545, 673
BstBI TTCGAA 2 cut(s) 154, 850
BstC8I GCNNGC 6 cut(s) 7, 240, 615, 1137, 1238, 1452
BstDEI CTNAG 7 cut(s) 125, 730, 789, 840, 927, 1079, 1259
BstDSI CCRYGG 1 cut(s) 41
BstF5I GGATG 4 cut(s) 129, 877, 1311, 1523
BstHHI GCGC 1 cut(s) 391
BstKTI GATC 8 cut(s) 84, 795, 966, 1015, 1275, 1360, 1468, 1509
BstMAI GTCTC 3 cut(s) 733, 1175, 1255
BstMBI GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
BstMWI GCNNNNNNNGC 5 cut(s) 305, 527, 656, 671, 1092
BstNI CCWGG 2 cut(s) 1050, 1193
BstNSI RCATGY 3 cut(s) 466, 835, 1143
BstPAI GACNNNNGTC 1 cut(s) 822
BstSCI CCNGG 3 cut(s) 1048, 1191, 1373
BstSFI CTRYAG 3 cut(s) 260, 1219, 1484
BstSLI GKGCMC 1 cut(s) 301
BstV1I GCAGC 7 cut(s) 274, 317, 629, 998, 1001, 1357, 1498
BstX2I RGATCY 1 cut(s) 963
BstXI CCANNNNNNTGG 1 cut(s) 821
BstYI RGATCY 1 cut(s) 963
Bsu15I ATCGAT 1 cut(s) 795
BsuI GTATCC 1 cut(s) 488
BsuRI GGCC 4 cut(s) 5, 510, 665, 1350
BsuTUI ATCGAT 1 cut(s) 795
BtgI CCRYGG 1 cut(s) 41
BtgZI GCGATG 1 cut(s) 1495
BtsCI GGATG 4 cut(s) 129, 877, 1311, 1523
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 2 cut(s) 837, 918
Cac8I GCNNGC 6 cut(s) 7, 240, 615, 1137, 1238, 1452
CciI TCATGA 1 cut(s) 78
CfoI GCGC 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 14, 811
ClaI ATCGAT 1 cut(s) 795
Csp6I GTAC 2 cut(s) 430, 1143
CviAII CATG 8 cut(s) 42, 79, 271, 463, 621, 783, 832, 1140
CviQI GTAC 2 cut(s) 430, 1143
DdeI CTNAG 7 cut(s) 125, 730, 789, 840, 927, 1079, 1259
DpnI GATC 8 cut(s) 83, 794, 965, 1014, 1274, 1359, 1467, 1508
DpnII GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
EaeI YGGCCR 3 cut(s) 3, 508, 1348
Eam1104I CTCTTC 2 cut(s) 545, 673
EarI CTCTTC 2 cut(s) 545, 673
EciI GGCGGA 3 cut(s) 170, 553, 661
Eco130I CCWWGG 4 cut(s) 41, 328, 345, 1311
Eco31I GGTCTC 2 cut(s) 733, 1255
Eco47I GGWCC 2 cut(s) 14, 811
Eco57I CTGAAG 2 cut(s) 211, 724
Eco88I CYCGRG 1 cut(s) 884
EcoRI GAATTC 1 cut(s) 502
EcoRII CCWGG 2 cut(s) 1048, 1191
EcoT14I CCWWGG 4 cut(s) 41, 328, 345, 1311
EcoT22I ATGCAT 1 cut(s) 288
ErhI CCWWGG 4 cut(s) 41, 328, 345, 1311
FaeI CATG 8 cut(s) 45, 82, 274, 466, 624, 786, 835, 1143
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FatI CATG 8 cut(s) 41, 78, 270, 462, 620, 782, 831, 1139
FauI CCCGC 3 cut(s) 648, 684, 1262
FbaI TGATCA 1 cut(s) 81
FblI GTMKAC 1 cut(s) 324
FokI GGATG 4 cut(s) 116, 884, 1318, 1530
FspBI CTAG 4 cut(s) 218, 453, 491, 518
FspI TGCGCA 1 cut(s) 390
GlaI GCGC 1 cut(s) 390
GsaI CCCAGC 1 cut(s) 1401
GsuI CTGGAG 1 cut(s) 561
HaeIII GGCC 4 cut(s) 5, 510, 665, 1350
HapII CCGG 3 cut(s) 193, 507, 1374
HhaI GCGC 1 cut(s) 391
Hin1II CATG 8 cut(s) 45, 82, 274, 466, 624, 786, 835, 1143
Hin6I GCGC 1 cut(s) 389
HinP1I GCGC 1 cut(s) 389
HinfI GANTC 5 cut(s) 21, 341, 1017, 1214, 1522
HpaII CCGG 3 cut(s) 193, 507, 1374
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 1 cut(s) 325
Hpy188I TCNGA 6 cut(s) 371, 709, 733, 790, 963, 1102
Hpy188III TCNNGA 7 cut(s) 32, 79, 193, 453, 809, 827, 1169
Hpy8I GTNNAC 1 cut(s) 325
HpyAV CCTTC 2 cut(s) 85, 459
HpyCH4III ACNGT 4 cut(s) 188, 974, 1111, 1223
HpyF10VI GCNNNNNNNGC 5 cut(s) 305, 527, 656, 671, 1092
HpyF3I CTNAG 7 cut(s) 125, 730, 789, 840, 927, 1079, 1259
Hsp92II CATG 8 cut(s) 45, 82, 274, 466, 624, 786, 835, 1143
HspAI GCGC 1 cut(s) 389
Kpn2I TCCGGA 1 cut(s) 192
Ksp22I TGATCA 1 cut(s) 81
Kzo9I GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
LmnI GCTCC 1 cut(s) 316
Lsp1109I GCAGC 7 cut(s) 274, 317, 629, 998, 1001, 1357, 1498
LweI GCATC 5 cut(s) 138, 433, 932, 1058, 1303
MaeI CTAG 4 cut(s) 218, 453, 491, 518
MaeIII GTNAC 3 cut(s) 266, 1328, 1411
MalI GATC 8 cut(s) 83, 794, 965, 1014, 1274, 1359, 1467, 1508
MbiI CCGCTC 1 cut(s) 311
MboI GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
MboII GAAGA 5 cut(s) 362, 562, 660, 992, 1330
MfeI CAATTG 2 cut(s) 1161, 1267
MflI RGATCY 1 cut(s) 963
MhlI GDGCHC 2 cut(s) 301, 406
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 395, 444, 502, 556, 582, 845, 1036, 1161, 1267
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 350
MmeI TCCRAC 1 cut(s) 968
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 288
MroI TCCGGA 1 cut(s) 192
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 1032, 1040, 1113
MslI CAYNNNNRTG 1 cut(s) 650
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 1489
MspI CCGG 3 cut(s) 193, 507, 1374
MspR9I CCNGG 3 cut(s) 1050, 1193, 1375
MunI CAATTG 2 cut(s) 1161, 1267
Mva1269I GAATGC 1 cut(s) 615
MvaI CCWGG 2 cut(s) 1050, 1193
MwoI GCNNNNNNNGC 5 cut(s) 305, 527, 656, 671, 1092
NciI CCSGG 1 cut(s) 1375
NcoI CCATGG 1 cut(s) 41
NdeII GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
NlaIII CATG 8 cut(s) 45, 82, 274, 466, 624, 786, 835, 1143
NlaIV GGNNCC 3 cut(s) 298, 364, 1252
NmeAIII GCCGAG 2 cut(s) 592, 1004
NmuCI GTSAC 1 cut(s) 1411
NsbI TGCGCA 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 288
NspI RCATGY 3 cut(s) 466, 835, 1143
NspV TTCGAA 2 cut(s) 154, 850
PaeR7I CTCGAG 1 cut(s) 884
PagI TCATGA 1 cut(s) 78
PctI GAATGC 1 cut(s) 615
PfeI GAWTC 4 cut(s) 21, 1017, 1214, 1522
PflMI CCANNNNNTGG 1 cut(s) 710
PfoI TCCNGGA 1 cut(s) 1048
PleI GAGTC 1 cut(s) 349
PpsI GAGTC 1 cut(s) 349
PshAI GACNNNNGTC 1 cut(s) 822
Psp6I CCWGG 2 cut(s) 1048, 1191
PspFI CCCAGC 1 cut(s) 1397
PspGI CCWGG 2 cut(s) 1048, 1191
PspN4I GGNNCC 3 cut(s) 298, 364, 1252
PspPI GGNCC 2 cut(s) 14, 811
PspXI VCTCGAGB 1 cut(s) 884
PstI CTGCAG 2 cut(s) 264, 1488
PsuI RGATCY 1 cut(s) 963
RsaI GTAC 2 cut(s) 431, 1144
RsaNI GTAC 2 cut(s) 430, 1143
RseI CAYNNNNRTG 1 cut(s) 650
SaqAI TTAA 3 cut(s) 1032, 1040, 1113
Sau3AI GATC 8 cut(s) 81, 792, 963, 1012, 1272, 1357, 1465, 1506
Sau96I GGNCC 2 cut(s) 14, 811
SchI GAGTC 1 cut(s) 350
ScrFI CCNGG 3 cut(s) 1050, 1193, 1375
SduI GDGCHC 2 cut(s) 301, 406
SfaNI GCATC 5 cut(s) 138, 433, 932, 1058, 1303
SfcI CTRYAG 3 cut(s) 260, 1219, 1484
Sfr274I CTCGAG 1 cut(s) 884
SfuI TTCGAA 2 cut(s) 154, 850
SinI GGWCC 2 cut(s) 14, 811
SlaI CTCGAG 1 cut(s) 884
SmiMI CAYNNNNRTG 1 cut(s) 650
SmlI CTYRAG 3 cut(s) 106, 884, 1422
SmoI CTYRAG 3 cut(s) 106, 884, 1422
Sse9I AATT 9 cut(s) 395, 444, 502, 556, 582, 845, 1036, 1161, 1267
SspMI CTAG 4 cut(s) 218, 453, 491, 518
StyD4I CCNGG 3 cut(s) 1048, 1191, 1373
StyI CCWWGG 4 cut(s) 41, 328, 345, 1311
TaaI ACNGT 4 cut(s) 188, 974, 1111, 1223
TasI AATT 9 cut(s) 395, 444, 502, 556, 582, 845, 1036, 1161, 1267
TatI WGTACW 1 cut(s) 1142
TauI GCSGC 3 cut(s) 311, 1236, 1353
TfiI GAWTC 4 cut(s) 21, 1017, 1214, 1522
Tru1I TTAA 3 cut(s) 1032, 1040, 1113
Tru9I TTAA 3 cut(s) 1032, 1040, 1113
TscAI CASTG 2 cut(s) 844, 925
TseFI GTSAC 1 cut(s) 1411
TseI GCWGC 7 cut(s) 262, 305, 617, 986, 989, 1370, 1486
Tsp45I GTSAC 1 cut(s) 1411
TspDTI ATGAA 3 cut(s) 67, 707, 894
TspRI CASTG 2 cut(s) 844, 925
Van91I CCANNNNNTGG 1 cut(s) 710
VpaK11BI GGWCC 2 cut(s) 14, 811
XapI RAATTY 4 cut(s) 395, 502, 556, 845
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 3 cut(s) 466, 835, 1143
XhoI CTCGAG 1 cut(s) 884
XmiI GTMKAC 1 cut(s) 324
XspI CTAG 4 cut(s) 218, 453, 491, 518
Zsp2I ATGCAT 1 cut(s) 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.