Rroxscaffold_1G00028350

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0017520
Physical Location & Seq
Forward (+)
36125195 .. 36127628
2434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028350.1

Sequence Viewer

Length: 1404 bp
ATGTCCAGCACAGCTCCAAGAATCCAAGCTCTTGACCTTCCATGGCTACTACTACTGTTTTTCGTCTTTTCAACCTTCATCATCAGTTCTTGTTCTTCTCCCTTGAGAAAAATACCGAGGCTCCGCATCCTACAAAGACCTATAACTTTTCGAAACCACAGCACTTCTGTTGCTGCCCGCCACCATTTCAGGACGTACTATTATGCTCAAACGCTAGACCACTTCAACTACTGGCCAGGGAGTTATGCCACCTTTCTGCAGCGTTACATGGTAAGTTCTATGCACTGGAGAGGTGCCGGTGCAGCCGCTCCTATGTTCGTGTACCTTGGTGGGGAGGAGTCCTTGGACGAAAGTATTGGCTCCATCGGATTTCTCACTGATAATGCGCCGAAATTTGGTGCTTTAATGGTATATATAGAGCATCGGTACTATGGGAAATCAATTCCATCTAGACCGAACATGCAGGATATATACAATGCTACCAGCCTCGGATATCTGAACTCCGCGCAAGCTCTGGCCGATTATGCAGAAATTATACTACACTTGAAGAGGAATTTATCGGCCGATGCTTCTCCAGTAATTGTCATTGGAGGCTCTTATGGGGGAATGCTTGCAGCATGGTTTCGCCTCAAATACCCGCACATTGCTGTGGGGGCATTGGCCTCTTCCGCGCCACTTCTTTACTTTGATGATATTACACCATCAGATGGGCTTCATTCCGTGGTCACAAAGAATTTCAGGGAAGCGAGTGAGAACTGCTACAATACCATAAAGAAATCTTGGTCTGAAATCGATAGAATCTCTGTTCACCAAGACGATGGTCTTGACATCCTCACTGAGAAATTTCAAACTTGCGAACCTTTGGAGGATGGAGATGAACTCGAGCTAAAGGCCTACTTGGAGTTCATATATATAACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAATCAGATCTGCAACAGTATAAATGCAGCAGCAGACAGAGGAGATGATATCATTGATCGAATTGCCTCGGCTGTTAATTATACTCGTCGGGGAGAGAATAGAGGTCAATGCTTGGACCTAAGTAGCGAACTTCGCCCATCAGATGATGCAGTGGATGGTTTGAACTGGTTATGGCAGCATATAAGGATGACCTTGGAGAGGTTTGCCAGTAACATCATCTTCTCAAATGGCCAGCGTGATCCTTTTAGCGTTGCCGGAGTATTGACAAATATATCGCCCAGCATAGTAGCCGTGACTACACGTCAAGGTTCTCATTGCTTGGACTTATTACCTGCGAGGATAAACGATCCCAGATGGTTAGTTGTGCAGCGACACATAGAAGTTGGGATTATCACAGGATGGATTCGCAACTACTACAATGATTTGCGTATCTTTTCTTCTGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

467

Amino Acids

52.71

Weight (kDa)

6.01

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 70 - 377 2.9e-59 Serine carboxypeptidase S28
Peptidase_S28 PF05577 387 - 443 2.5e-09 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1294
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 707
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 2 cut(s) 506, 671
AciI CCGC 6 cut(s) 124, 178, 306, 504, 638, 669
AclWI GGATC 2 cut(s) 1187, 1295
AcoI YGGCCR 4 cut(s) 233, 516, 561, 1183
AcsI RAATTY 4 cut(s) 392, 553, 733, 842
AfaI GTAC 3 cut(s) 197, 323, 428
AfiI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1152
AflIII ACRYGT 1 cut(s) 1253
AgsI TTSAA 5 cut(s) 72, 226, 547, 848, 1117
AhdI GACNNNNNGTC 1 cut(s) 1254
AjiI CACGTC 1 cut(s) 1256
AjnI CCWGG 1 cut(s) 235
AluBI AGCT 4 cut(s) 14, 29, 512, 886
AluI AGCT 4 cut(s) 14, 29, 512, 886
AlwI GGATC 2 cut(s) 1187, 1295
Ama87I CYCGRG 1 cut(s) 881
AoxI GGCC 6 cut(s) 233, 516, 561, 660, 891, 1183
ApeKI GCWGC 8 cut(s) 173, 259, 302, 614, 980, 983, 1129, 1321
ApoI RAATTY 4 cut(s) 392, 553, 733, 842
AspLEI GCGC 3 cut(s) 388, 508, 673
AspS9I GGNCC 1 cut(s) 1069
AsuHPI GGTGA 1 cut(s) 800
AsuII TTCGAA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 1 cut(s) 1069
BalI TGGCCA 2 cut(s) 235, 1185
BanI GGYRCC 1 cut(s) 293
BarI GAAGNNNNNNTAC 1 cut(s) 1375
BbvI GCAGC 8 cut(s) 160, 271, 314, 626, 992, 995, 1141, 1333
BceAI ACGGC 1 cut(s) 1229
BciT130I CCWGG 1 cut(s) 237
BfaI CTAG 2 cut(s) 215, 450
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 1294
BglII AGATCT 1 cut(s) 960
BisI GCNGC 9 cut(s) 174, 260, 303, 306, 615, 981, 984, 1130, 1322
BlsI GCNGC 9 cut(s) 175, 261, 304, 307, 616, 982, 985, 1131, 1323
Bme1390I CCNGG 1 cut(s) 237
Bme18I GGWCC 1 cut(s) 1069
BmeRI GACNNNNNGTC 1 cut(s) 1254
BmeT110I CYCGRG 1 cut(s) 881
BmgBI CACGTC 1 cut(s) 1256
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 3 cut(s) 122, 295, 361
BmrFI CCNGG 1 cut(s) 237
BmsI GCATC 5 cut(s) 135, 430, 556, 929, 1090
BoxI GACNNNNGTC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 2 cut(s) 307, 558
Bpu14I TTCGAA 1 cut(s) 151
BpuEI CTTGAG 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 792
BsaJI CCNNGG 9 cut(s) 41, 116, 236, 325, 342, 487, 720, 1020, 1146
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1152
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 6 cut(s) 236, 290, 575, 950, 1124, 1161
Bse3DI GCAATG 2 cut(s) 642, 1267
BseBI CCWGG 1 cut(s) 237
BseCI ATCGAT 1 cut(s) 792
BseDI CCNNGG 9 cut(s) 41, 116, 236, 325, 342, 487, 720, 1020, 1146
BseGI GGATG 6 cut(s) 126, 828, 874, 1114, 1146, 1358
BseLI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1152
BseMI GCAATG 2 cut(s) 642, 1267
BseMII CTCAG 3 cut(s) 828, 938, 1386
BseNI ACTGG 6 cut(s) 236, 290, 575, 950, 1124, 1161
BseRI GAGGAG 2 cut(s) 350, 1008
BseX3I CGGCCG 1 cut(s) 561
BseXI GCAGC 8 cut(s) 160, 271, 314, 626, 992, 995, 1141, 1333
BseYI CCCAGC 1 cut(s) 1232
BsgI GTGCAG 2 cut(s) 321, 1340
Bsh1236I CGCG 2 cut(s) 506, 671
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 6 cut(s) 235, 518, 563, 662, 893, 1185
BshNI GGYRCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 792
BsiEI CGRYCG 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 2 cut(s) 297, 1209
BslI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1152
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 6 cut(s) 235, 518, 563, 662, 893, 1185
BsoBI CYCGRG 1 cut(s) 881
Bsp119I TTCGAA 1 cut(s) 151
Bsp143I GATC 4 cut(s) 960, 1009, 1192, 1300
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 6 cut(s) 124, 178, 306, 504, 638, 669
BspANI GGCC 6 cut(s) 235, 518, 563, 662, 893, 1185
BspCNI CTCAG 3 cut(s) 829, 937, 1387
BspDI ATCGAT 1 cut(s) 792
BspFNI CGCG 2 cut(s) 506, 671
BspLI GGNNCC 3 cut(s) 122, 295, 361
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 1294
BspPI GGATC 2 cut(s) 1187, 1295
BspT104I TTCGAA 1 cut(s) 151
BspT107I GGYRCC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 642, 1267
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 6 cut(s) 236, 290, 575, 950, 1124, 1161
BssAI RCCGGY 1 cut(s) 296
BssECI CCNNGG 9 cut(s) 41, 116, 236, 325, 342, 487, 720, 1020, 1146
BssMI GATC 4 cut(s) 960, 1009, 1192, 1300
BssT1I CCWWGG 4 cut(s) 41, 325, 342, 1146
Bst2UI CCWGG 1 cut(s) 237
Bst4CI ACNGT 2 cut(s) 57, 971
Bst6I CTCTTC 2 cut(s) 542, 670
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 4 cut(s) 178, 510, 612, 1187
BstDEI CTNAG 4 cut(s) 837, 924, 1073, 1395
BstDSI CCRYGG 2 cut(s) 41, 720
BstENI CCTNNNNNAGG 1 cut(s) 1150
BstF5I GGATG 6 cut(s) 126, 828, 874, 1114, 1146, 1358
BstFNI CGCG 2 cut(s) 506, 671
BstHHI GCGC 3 cut(s) 388, 508, 673
BstKTI GATC 4 cut(s) 963, 1012, 1195, 1303
BstMBI GATC 4 cut(s) 960, 1009, 1192, 1300
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 5 cut(s) 302, 524, 653, 668, 1086
BstNI CCWGG 1 cut(s) 237
BstNSI RCATGY 1 cut(s) 463
BstPAI GACNNNNGTC 1 cut(s) 819
BstSCI CCNGG 1 cut(s) 235
BstSFI CTRYAG 1 cut(s) 257
BstUI CGCG 2 cut(s) 506, 671
BstV1I GCAGC 8 cut(s) 160, 271, 314, 626, 992, 995, 1141, 1333
BstX2I RGATCY 1 cut(s) 960
BstXI CCANNNNNNTGG 1 cut(s) 818
BstYI RGATCY 1 cut(s) 960
BstZI CGGCCG 1 cut(s) 561
Bsu15I ATCGAT 1 cut(s) 792
BsuRI GGCC 6 cut(s) 235, 518, 563, 662, 893, 1185
BsuTUI ATCGAT 1 cut(s) 792
BtgI CCRYGG 2 cut(s) 41, 720
BtrI CACGTC 1 cut(s) 1256
BtsCI GGATG 6 cut(s) 126, 828, 874, 1114, 1146, 1358
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 4 cut(s) 283, 375, 834, 1110
BveI ACCTGC 1 cut(s) 1294
Cac8I GCNNGC 4 cut(s) 178, 510, 612, 1187
CfoI GCGC 3 cut(s) 388, 508, 673
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 1069
ClaI ATCGAT 1 cut(s) 792
Csp6I GTAC 3 cut(s) 196, 322, 427
CviAII CATG 4 cut(s) 42, 268, 460, 618
CviQI GTAC 3 cut(s) 196, 322, 427
DdeI CTNAG 4 cut(s) 837, 924, 1073, 1395
DpnI GATC 4 cut(s) 962, 1011, 1194, 1302
DpnII GATC 4 cut(s) 960, 1009, 1192, 1300
DriI GACNNNNNGTC 1 cut(s) 1254
EaeI YGGCCR 4 cut(s) 233, 516, 561, 1183
EagI CGGCCG 1 cut(s) 561
Eam1104I CTCTTC 2 cut(s) 542, 670
Eam1105I GACNNNNNGTC 1 cut(s) 1254
EarI CTCTTC 2 cut(s) 542, 670
EclXI CGGCCG 1 cut(s) 561
Eco130I CCWWGG 4 cut(s) 41, 325, 342, 1146
Eco147I AGGCCT 1 cut(s) 893
Eco32I GATATC 2 cut(s) 494, 1003
Eco47I GGWCC 1 cut(s) 1069
Eco52I CGGCCG 1 cut(s) 561
Eco88I CYCGRG 1 cut(s) 881
EcoNI CCTNNNNNAGG 1 cut(s) 1150
EcoRII CCWGG 1 cut(s) 235
EcoRV GATATC 2 cut(s) 494, 1003
EcoT14I CCWWGG 4 cut(s) 41, 325, 342, 1146
ErhI CCWWGG 4 cut(s) 41, 325, 342, 1146
FaeI CATG 4 cut(s) 45, 271, 463, 621
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FatI CATG 4 cut(s) 41, 267, 459, 617
FauI CCCGC 2 cut(s) 185, 645
Fnu4HI GCNGC 9 cut(s) 174, 260, 303, 306, 615, 981, 984, 1130, 1322
FokI GGATG 6 cut(s) 113, 815, 881, 1121, 1153, 1365
Fsp4HI GCNGC 9 cut(s) 174, 260, 303, 306, 615, 981, 984, 1130, 1322
FspBI CTAG 2 cut(s) 215, 450
GlaI GCGC 3 cut(s) 387, 507, 672
GluI GCNGC 9 cut(s) 174, 260, 303, 306, 615, 981, 984, 1130, 1322
GsaI CCCAGC 1 cut(s) 1236
GsuI CTGGAG 2 cut(s) 307, 558
HaeIII GGCC 6 cut(s) 235, 518, 563, 662, 893, 1185
HapII CCGG 2 cut(s) 297, 1209
HhaI GCGC 3 cut(s) 388, 508, 673
Hin1II CATG 4 cut(s) 45, 271, 463, 621
Hin6I GCGC 3 cut(s) 386, 506, 671
HinP1I GCGC 3 cut(s) 386, 506, 671
HinfI GANTC 4 cut(s) 21, 338, 798, 1357
HpaII CCGG 2 cut(s) 297, 1209
HphI GGTGA 1 cut(s) 800
Hpy166II GTNNAC 2 cut(s) 322, 808
Hpy188I TCNGA 8 cut(s) 368, 491, 498, 706, 787, 960, 1096, 1396
Hpy188III TCNNGA 4 cut(s) 32, 190, 450, 824
Hpy8I GTNNAC 2 cut(s) 322, 808
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 2 cut(s) 47, 85
HpyCH4III ACNGT 2 cut(s) 57, 971
HpyCH4IV ACGT 2 cut(s) 194, 1255
HpyF10VI GCNNNNNNNGC 5 cut(s) 302, 524, 653, 668, 1086
HpyF3I CTNAG 4 cut(s) 837, 924, 1073, 1395
HpySE526I ACGT 2 cut(s) 194, 1255
Hsp92II CATG 4 cut(s) 45, 271, 463, 621
HspAI GCGC 3 cut(s) 386, 506, 671
Kzo9I GATC 4 cut(s) 960, 1009, 1192, 1300
LmnI GCTCC 4 cut(s) 19, 126, 313, 365
Lsp1109I GCAGC 8 cut(s) 160, 271, 314, 626, 992, 995, 1141, 1333
LweI GCATC 5 cut(s) 135, 430, 556, 929, 1090
MaeI CTAG 2 cut(s) 215, 450
MaeII ACGT 2 cut(s) 194, 1255
MaeIII GTNAC 4 cut(s) 263, 724, 1163, 1246
MalI GATC 4 cut(s) 962, 1011, 1194, 1302
MbiI CCGCTC 1 cut(s) 308
MboI GATC 4 cut(s) 960, 1009, 1192, 1300
MboII GAAGA 5 cut(s) 87, 559, 657, 1165, 1383
MflI RGATCY 1 cut(s) 960
MlsI TGGCCA 2 cut(s) 235, 1185
MluCI AATT 9 cut(s) 392, 441, 531, 553, 579, 733, 842, 1014, 1030
MluNI TGGCCA 2 cut(s) 235, 1185
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 965
Mox20I TGGCCA 2 cut(s) 235, 1185
MscI TGGCCA 2 cut(s) 235, 1185
MseI TTAA 2 cut(s) 404, 1029
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 2 cut(s) 235, 1185
MspI CCGG 2 cut(s) 297, 1209
MspR9I CCNGG 1 cut(s) 237
Mva1269I GAATGC 1 cut(s) 612
MvaI CCWGG 1 cut(s) 237
MvnI CGCG 2 cut(s) 506, 671
MwoI GCNNNNNNNGC 5 cut(s) 302, 524, 653, 668, 1086
NcoI CCATGG 1 cut(s) 41
NdeII GATC 4 cut(s) 960, 1009, 1192, 1300
NlaIII CATG 4 cut(s) 45, 271, 463, 621
NlaIV GGNNCC 3 cut(s) 122, 295, 361
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 2 cut(s) 724, 1246
NspI RCATGY 1 cut(s) 463
NspV TTCGAA 1 cut(s) 151
PaeR7I CTCGAG 1 cut(s) 881
PceI AGGCCT 1 cut(s) 893
PctI GAATGC 1 cut(s) 612
PfeI GAWTC 3 cut(s) 21, 798, 1357
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 9 cut(s) 175, 261, 304, 307, 616, 982, 985, 1131, 1323
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PshAI GACNNNNGTC 1 cut(s) 819
Psp6I CCWGG 1 cut(s) 235
PspFI CCCAGC 1 cut(s) 1232
PspGI CCWGG 1 cut(s) 235
PspN4I GGNNCC 3 cut(s) 122, 295, 361
PspPI GGNCC 1 cut(s) 1069
PspXI VCTCGAGB 1 cut(s) 881
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 960
RsaI GTAC 3 cut(s) 197, 323, 428
RsaNI GTAC 3 cut(s) 196, 322, 427
RseI CAYNNNNRTG 1 cut(s) 647
SaqAI TTAA 2 cut(s) 404, 1029
SatI GCNGC 9 cut(s) 174, 260, 303, 306, 615, 981, 984, 1130, 1322
Sau3AI GATC 4 cut(s) 960, 1009, 1192, 1300
Sau96I GGNCC 1 cut(s) 1069
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 237
SfaNI GCATC 5 cut(s) 135, 430, 556, 929, 1090
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 1 cut(s) 881
SfuI TTCGAA 1 cut(s) 151
SinI GGWCC 1 cut(s) 1069
SlaI CTCGAG 1 cut(s) 881
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 103, 881
SmoI CTYRAG 2 cut(s) 103, 881
Sse9I AATT 9 cut(s) 392, 441, 531, 553, 579, 733, 842, 1014, 1030
SseBI AGGCCT 1 cut(s) 893
SsiI CCGC 6 cut(s) 124, 178, 306, 504, 638, 669
SspMI CTAG 2 cut(s) 215, 450
StuI AGGCCT 1 cut(s) 893
StyD4I CCNGG 1 cut(s) 235
StyI CCWWGG 4 cut(s) 41, 325, 342, 1146
TaaI ACNGT 2 cut(s) 57, 971
TaiI ACGT 2 cut(s) 197, 1258
TaqI TCGA 4 cut(s) 151, 792, 882, 1012
TaqII GACCGA 1 cut(s) 469
TasI AATT 9 cut(s) 392, 441, 531, 553, 579, 733, 842, 1014, 1030
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 3 cut(s) 21, 798, 1357
Tru1I TTAA 2 cut(s) 404, 1029
Tru9I TTAA 2 cut(s) 404, 1029
TscAI CASTG 4 cut(s) 290, 382, 841, 1110
TseFI GTSAC 2 cut(s) 724, 1246
TseI GCWGC 8 cut(s) 173, 259, 302, 614, 980, 983, 1129, 1321
Tsp45I GTSAC 2 cut(s) 724, 1246
TspDTI ATGAA 4 cut(s) 67, 704, 891, 895
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 4 cut(s) 290, 382, 841, 1110
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 1069
XagI CCTNNNNNAGG 1 cut(s) 1150
XapI RAATTY 4 cut(s) 392, 553, 733, 842
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 1 cut(s) 463
XhoI CTCGAG 1 cut(s) 881
XspI CTAG 2 cut(s) 215, 450
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.