Rw5G032520

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017520
Physical Location & Seq
Reverse (-)
55106294 .. 55112700
6407 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G032520.1

Sequence Viewer

Length: 1020 bp
ATGAAATTCTCAAGAAACATAGACACCACAACGAGATTTGAGGCGTTTACGACTGCTATGAGAGGATTAAGTTCCCCTTCCCCAAGAAGGACTGCTCTCACCGCCGATGCCTATCTCTCGCTCATCACCACCTCCAGAGTGCTTGCAGCATGGTTTCGCCTCAAATACCCGCACATTGTTGTGCGGGCATTGGCCTCTTCCGCCCCACTTCTTTACTTTGATGACATTACACCATCAGATGGGCTTCATTCCGTGGTCACAAACAATTTCAGGGAAGCGAGTGAGAACTGCTACAATACCATAAAGAAATCATGGTCTGAAATCGATAGAATTGTTCCAGCAAGACGATGGCCTACTTGGATTATTTATATAACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAATCAGATCTGCAACAGTATAAATGCAGCAGCAGACAGAGGAGATGATATCATTGATCGAATTGCCTCGGCTGTTAATTATACTCGTCGAGGAGAGAATAGAGGTCAATGCTTGGACCTAAGTAGCGAACTTCACCCATCAGATGATGCAGTGGATGGTTTGAACTGGTTATGGCAGGCATGTACTGAAATTTTTATGCCAATCGGTCGAGAAACGGACGAGACTATGTTTCAGGCAGAACCATTTGATGCGGATTCCTACACTCAAAGGTGCCACCAGTTATATCAAGGTGCCCGCCCTAGACCCCATTGGATCACAACTTATTATGGGGGGCATCATATAAGGATGACCTTGGAGAGGTTTGCCAGTAACATCATCTTCTCAAATGGCCAGCGTGATCCTTTTAGCGTTGCCGGAGTATTGACAAATATATTGCCCAGCATAGTTGCCGTGACTACACGTCAAGGTTCTCATTGCTTGGACTTATTACCTGCAAGGATAAATGATCCCAGATGGTTAGTTGTGCAGCGACACATAGAAGTTGGGATTATCACAGGATGCATTCGCAACTACTACAATGATTTGCGTATCTTTTCTTCTGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

339

Amino Acids

38.6

Weight (kDa)

6.75

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 47 - 112 3.8e-12 Serine carboxypeptidase S28
Peptidase_S28 PF05577 133 - 315 7.9e-21 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 910
AccB1I GGYRCC 2 cut(s) 681, 701
AccB7I CCANNNNNTGG 1 cut(s) 239
AciI CCGC 6 cut(s) 102, 170, 184, 201, 662, 706
AclWI GGATC 3 cut(s) 731, 803, 911
AcoI YGGCCR 1 cut(s) 799
AcsI RAATTY 2 cut(s) 5, 600
AfaI GTAC 1 cut(s) 595
AfiI CCNNNNNNNGG 3 cut(s) 87, 239, 768
AflIII ACRYGT 1 cut(s) 869
AgsI TTSAA 1 cut(s) 574
AhdI GACNNNNNGTC 1 cut(s) 870
AjiI CACGTC 1 cut(s) 872
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 3 cut(s) 731, 803, 911
AoxI GGCC 3 cut(s) 192, 350, 799
ApeKI GCWGC 4 cut(s) 146, 437, 440, 937
ApoI RAATTY 2 cut(s) 5, 600
AspS9I GGNCC 1 cut(s) 526
AsuHPI GGTGA 3 cut(s) 91, 118, 536
AvaII GGWCC 1 cut(s) 526
BaeGI GKGCMC 1 cut(s) 706
BalI TGGCCA 1 cut(s) 801
BanI GGYRCC 2 cut(s) 681, 701
BarI GAAGNNNNNNTAC 1 cut(s) 991
BbvI GCAGC 4 cut(s) 158, 449, 452, 949
BccI CCATC 6 cut(s) 233, 241, 342, 556, 560, 918
BceAI ACGGC 1 cut(s) 845
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 1 cut(s) 711
BfuAI ACCTGC 1 cut(s) 910
BglII AGATCT 1 cut(s) 417
BisI GCNGC 4 cut(s) 147, 438, 441, 938
BlsI GCNGC 4 cut(s) 148, 439, 442, 939
Bme18I GGWCC 1 cut(s) 526
BmeRI GACNNNNNGTC 1 cut(s) 870
BmgBI CACGTC 1 cut(s) 872
BmgT120I GGNCC 1 cut(s) 526
BmiI GGNNCC 2 cut(s) 683, 703
BmsI GCATC 6 cut(s) 97, 386, 547, 649, 754, 959
BpmI CTGGAG 1 cut(s) 118
Bsa29I ATCGAT 1 cut(s) 324
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 3 cut(s) 252, 477, 762
Bsc4I CCNNNNNNNGG 3 cut(s) 87, 239, 768
Bse1I ACTGG 4 cut(s) 407, 581, 688, 777
Bse3DI GCAATG 1 cut(s) 883
Bse8I GATNNNNATC 1 cut(s) 111
BseCI ATCGAT 1 cut(s) 324
BseDI CCNNGG 3 cut(s) 252, 477, 762
BseGI GGATG 3 cut(s) 571, 762, 974
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 3 cut(s) 87, 239, 768
BseMI GCAATG 1 cut(s) 883
BseMII CTCAG 2 cut(s) 395, 1002
BseNI ACTGG 4 cut(s) 407, 581, 688, 777
BseRI GAGGAG 2 cut(s) 465, 516
BseSI GKGCMC 1 cut(s) 706
BseXI GCAGC 4 cut(s) 158, 449, 452, 949
BseYI CCCAGC 1 cut(s) 848
BsgI GTGCAG 1 cut(s) 956
Bsh1285I CGRYCG 1 cut(s) 619
BshFI GGCC 3 cut(s) 194, 352, 801
BshNI GGYRCC 2 cut(s) 681, 701
BshVI ATCGAT 1 cut(s) 324
BsiEI CGRYCG 1 cut(s) 619
BsiSI CCGG 1 cut(s) 825
BslI CCNNNNNNNGG 3 cut(s) 87, 239, 768
BsmAI GTCTC 1 cut(s) 626
BsmI GAATGC 1 cut(s) 972
BsnI GGCC 3 cut(s) 194, 352, 801
Bsp1286I GDGCHC 1 cut(s) 706
Bsp143I GATC 5 cut(s) 417, 466, 723, 808, 916
BspACI CCGC 6 cut(s) 102, 170, 184, 201, 662, 706
BspANI GGCC 3 cut(s) 194, 352, 801
BspCNI CTCAG 2 cut(s) 394, 1003
BspDI ATCGAT 1 cut(s) 324
BspLI GGNNCC 2 cut(s) 683, 703
BspMI ACCTGC 1 cut(s) 910
BspPI GGATC 3 cut(s) 731, 803, 911
BspT107I GGYRCC 2 cut(s) 681, 701
BsrDI GCAATG 1 cut(s) 883
BsrI ACTGG 4 cut(s) 407, 581, 688, 777
BssECI CCNNGG 3 cut(s) 252, 477, 762
BssMI GATC 5 cut(s) 417, 466, 723, 808, 916
BssT1I CCWWGG 1 cut(s) 762
Bst4CI ACNGT 1 cut(s) 428
Bst6I CTCTTC 1 cut(s) 202
BstC8I GCNNGC 5 cut(s) 144, 186, 588, 706, 803
BstDEI CTNAG 3 cut(s) 381, 530, 1011
BstDSI CCRYGG 1 cut(s) 252
BstENI CCTNNNNNAGG 1 cut(s) 766
BstF5I GGATG 3 cut(s) 571, 762, 974
BstKTI GATC 5 cut(s) 420, 469, 726, 811, 919
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 5 cut(s) 417, 466, 723, 808, 916
BstMCI CGRYCG 1 cut(s) 619
BstMWI GCNNNNNNNGC 2 cut(s) 101, 200
BstNSI RCATGY 1 cut(s) 594
BstSLI GKGCMC 1 cut(s) 706
BstV1I GCAGC 4 cut(s) 158, 449, 452, 949
BstX2I RGATCY 1 cut(s) 417
BstYI RGATCY 1 cut(s) 417
Bsu15I ATCGAT 1 cut(s) 324
BsuRI GGCC 3 cut(s) 194, 352, 801
BsuTUI ATCGAT 1 cut(s) 324
BtgI CCRYGG 1 cut(s) 252
BtrI CACGTC 1 cut(s) 872
BtsCI GGATG 3 cut(s) 571, 762, 974
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 567
BveI ACCTGC 1 cut(s) 910
Cac8I GCNNGC 5 cut(s) 144, 186, 588, 706, 803
Cfr13I GGNCC 1 cut(s) 526
ClaI ATCGAT 1 cut(s) 324
Csp6I GTAC 1 cut(s) 594
CviAII CATG 3 cut(s) 150, 312, 591
CviJI RGCY 5 cut(s) 194, 244, 352, 482, 801
CviKI_1 RGCY 5 cut(s) 194, 244, 352, 482, 801
CviQI GTAC 1 cut(s) 594
DdeI CTNAG 3 cut(s) 381, 530, 1011
DpnI GATC 5 cut(s) 419, 468, 725, 810, 918
DpnII GATC 5 cut(s) 417, 466, 723, 808, 916
DriI GACNNNNNGTC 1 cut(s) 870
EaeI YGGCCR 1 cut(s) 799
Eam1104I CTCTTC 1 cut(s) 202
Eam1105I GACNNNNNGTC 1 cut(s) 870
EarI CTCTTC 1 cut(s) 202
EciI GGCGGA 1 cut(s) 190
Eco130I CCWWGG 1 cut(s) 762
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 1 cut(s) 526
EcoNI CCTNNNNNAGG 1 cut(s) 766
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 1 cut(s) 762
EcoT22I ATGCAT 1 cut(s) 974
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 3 cut(s) 153, 315, 594
FalI AAGNNNNNCTT 2 cut(s) 61, 93
FatI CATG 3 cut(s) 149, 311, 590
FauI CCCGC 3 cut(s) 177, 177, 713
Fnu4HI GCNGC 4 cut(s) 147, 438, 441, 938
FokI GGATG 3 cut(s) 578, 769, 981
Fsp4HI GCNGC 4 cut(s) 147, 438, 441, 938
FspBI CTAG 1 cut(s) 711
GluI GCNGC 4 cut(s) 147, 438, 441, 938
GsaI CCCAGC 1 cut(s) 852
GsuI CTGGAG 1 cut(s) 118
HaeIII GGCC 3 cut(s) 194, 352, 801
HapII CCGG 1 cut(s) 825
Hin1II CATG 3 cut(s) 153, 315, 594
HinfI GANTC 1 cut(s) 665
HpaII CCGG 1 cut(s) 825
HphI GGTGA 3 cut(s) 91, 118, 536
Hpy166II GTNNAC 1 cut(s) 48
Hpy188I TCNGA 5 cut(s) 238, 319, 417, 553, 1012
Hpy188III TCNNGA 3 cut(s) 12, 135, 620
Hpy8I GTNNAC 1 cut(s) 48
Hpy99I CGWCG 1 cut(s) 501
HpyAV CCTTC 2 cut(s) 81, 87
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4IV ACGT 1 cut(s) 871
HpyCH4V TGCA 8 cut(s) 146, 377, 423, 437, 560, 905, 937, 972
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 200
HpyF3I CTNAG 3 cut(s) 381, 530, 1011
HpySE526I ACGT 1 cut(s) 871
Hsp92II CATG 3 cut(s) 153, 315, 594
Kzo9I GATC 5 cut(s) 417, 466, 723, 808, 916
Lsp1109I GCAGC 4 cut(s) 158, 449, 452, 949
LweI GCATC 6 cut(s) 97, 386, 547, 649, 754, 959
MaeI CTAG 1 cut(s) 711
MaeII ACGT 1 cut(s) 871
MaeIII GTNAC 3 cut(s) 256, 779, 862
MalI GATC 5 cut(s) 419, 468, 725, 810, 918
MboI GATC 5 cut(s) 417, 466, 723, 808, 916
MboII GAAGA 3 cut(s) 189, 781, 999
MflI RGATCY 1 cut(s) 417
MhlI GDGCHC 1 cut(s) 706
MlsI TGGCCA 1 cut(s) 801
MluCI AATT 6 cut(s) 5, 265, 330, 471, 487, 600
MluNI TGGCCA 1 cut(s) 801
MmeI TCCRAC 1 cut(s) 422
Mox20I TGGCCA 1 cut(s) 801
Mph1103I ATGCAT 1 cut(s) 974
MscI TGGCCA 1 cut(s) 801
MseI TTAA 2 cut(s) 68, 486
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 801
MspI CCGG 1 cut(s) 825
Mva1269I GAATGC 1 cut(s) 972
MwoI GCNNNNNNNGC 2 cut(s) 101, 200
NdeII GATC 5 cut(s) 417, 466, 723, 808, 916
NlaIII CATG 3 cut(s) 153, 315, 594
NlaIV GGNNCC 2 cut(s) 683, 703
NmeAIII GCCGAG 1 cut(s) 458
NmuCI GTSAC 2 cut(s) 256, 862
NsiI ATGCAT 1 cut(s) 974
NspI RCATGY 1 cut(s) 594
PctI GAATGC 1 cut(s) 972
PfeI GAWTC 1 cut(s) 665
PflMI CCANNNNNTGG 1 cut(s) 239
PkrI GCNGC 4 cut(s) 148, 439, 442, 939
PspFI CCCAGC 1 cut(s) 848
PspN4I GGNNCC 2 cut(s) 683, 703
PspPI GGNCC 1 cut(s) 526
PsuI RGATCY 1 cut(s) 417
RsaI GTAC 1 cut(s) 595
RsaNI GTAC 1 cut(s) 594
RseI CAYNNNNRTG 1 cut(s) 179
SaqAI TTAA 2 cut(s) 68, 486
SatI GCNGC 4 cut(s) 147, 438, 441, 938
Sau3AI GATC 5 cut(s) 417, 466, 723, 808, 916
Sau96I GGNCC 1 cut(s) 526
SduI GDGCHC 1 cut(s) 706
SfaNI GCATC 6 cut(s) 97, 386, 547, 649, 754, 959
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 10
SmoI CTYRAG 1 cut(s) 10
Sse9I AATT 6 cut(s) 5, 265, 330, 471, 487, 600
SsiI CCGC 6 cut(s) 102, 170, 184, 201, 662, 706
SspMI CTAG 1 cut(s) 711
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 1 cut(s) 428
TaiI ACGT 1 cut(s) 874
TaqI TCGA 4 cut(s) 324, 469, 499, 619
TaqII GACCGA 1 cut(s) 605
TasI AATT 6 cut(s) 5, 265, 330, 471, 487, 600
TatI WGTACW 1 cut(s) 593
TfiI GAWTC 1 cut(s) 665
Tru1I TTAA 2 cut(s) 68, 486
Tru9I TTAA 2 cut(s) 68, 486
TscAI CASTG 1 cut(s) 567
TseFI GTSAC 2 cut(s) 256, 862
TseI GCWGC 4 cut(s) 146, 437, 440, 937
Tsp45I GTSAC 2 cut(s) 256, 862
TspDTI ATGAA 2 cut(s) 17, 236
TspGWI ACGGA 2 cut(s) 241, 641
TspRI CASTG 1 cut(s) 567
Van91I CCANNNNNTGG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 526
XagI CCTNNNNNAGG 1 cut(s) 766
XapI RAATTY 2 cut(s) 5, 600
XceI RCATGY 1 cut(s) 594
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XspI CTAG 1 cut(s) 711
Zsp2I ATGCAT 1 cut(s) 974
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.