Rh5DG370700

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0017520
Physical Location & Seq
Reverse (-)
55797267 .. 55798821
1555 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG370700.1

Sequence Viewer

Length: 957 bp
ATGCAGGCAGCATGGTTTCGCCTCAAATACCCGCACATTGCTGTGGGGGCATTGGCCTCTTCCGCCCCACTTCTTTACTTTGATGACATTACACCATCAGATGGGCTTCATTCCGTGGTCACAAACAATTTCAGGGAAGCGAGTGAGAACTGCTACAATACCATAAAGAAATCATGGTCTGAAATCGATAGAATTGCTGTTCACCAAGACGATGGTCTTGACATCCTCACGGAGAAATTTCAAACTTGCGAACCTTTGGAGGATGGAGATGAACTCGAGCTAAAGGCCTACTTGGAGTTCATTTATATAACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAATCTGATCTGCAACAGTATAAATGCAGCAGCAGACAGAGGAGATGATATCATTGATCGAATTGCCTCGGCTGTTAATTATACTCGTCGAGGAGAGAATAGAGGTCAATGTTTGGACCTAAGTAGCGAACTTCGCCCATCAGATGATGCAGTGGATGGTTTGAACTGGTTATGGCAGGCATGTACTGAAATTTTTATGCCAAACGGTCGAGAAACGGACGAGACTATGTTTCAGGCAGAACCATTTGATGCGGATTCCTACACTCAAAGGTGCCACCAGTTATATCAAGGTGCCCGCCCTAGACCCCATTGGATCACAACTTATTATGGGGGGCATCATATAAGGATGACCTTGGAGAGGTCTGCCAGTAACATCATCTTCTCAAATGGCCAGCGTGATCCTTTTAGCGTTGCCGGAGTATTGACAAATATATCGCCCAGCATAGTAGCAGTGACTACACGTCAAGGTTCTCATTGCTTGGACTTATTACCTGCAAGGATAAATGATCCCAGGTGGTTAGTTGTGCAGCGACACATAGAAGTTGGGATTATCACAGGATGGATTCGCAACTACTACAATGATTTGCGTATCTTTTCTTCTGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

318

Amino Acids

36.14

Weight (kDa)

4.92

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 3 - 294 7.4e-42 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 847
AccB1I GGYRCC 2 cut(s) 618, 638
AccB7I CCANNNNNTGG 1 cut(s) 101
AciI CCGC 4 cut(s) 32, 63, 599, 643
AclWI GGATC 3 cut(s) 668, 740, 848
AcoI YGGCCR 1 cut(s) 736
AcsI RAATTY 2 cut(s) 236, 537
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 2 cut(s) 101, 705
AflIII ACRYGT 1 cut(s) 806
AgsI TTSAA 2 cut(s) 242, 511
AhdI GACNNNNNGTC 1 cut(s) 807
AjiI CACGTC 1 cut(s) 809
AjnI CCWGG 1 cut(s) 857
AluBI AGCT 1 cut(s) 280
AluI AGCT 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 563
AlwI GGATC 3 cut(s) 668, 740, 848
Ama87I CYCGRG 1 cut(s) 275
AoxI GGCC 3 cut(s) 54, 285, 736
ApeKI GCWGC 4 cut(s) 8, 374, 377, 874
ApoI RAATTY 2 cut(s) 236, 537
AspS9I GGNCC 1 cut(s) 463
AsuHPI GGTGA 1 cut(s) 194
AvaI CYCGRG 1 cut(s) 275
AvaII GGWCC 1 cut(s) 463
BaeGI GKGCMC 1 cut(s) 643
BalI TGGCCA 1 cut(s) 738
BanI GGYRCC 2 cut(s) 618, 638
BarI GAAGNNNNNNTAC 1 cut(s) 928
BbvI GCAGC 4 cut(s) 20, 386, 389, 886
BccI CCATC 7 cut(s) 95, 103, 206, 257, 493, 497, 900
BcgI CGANNNNNNTGC 2 cut(s) 176, 210
BciT130I CCWGG 1 cut(s) 859
BcoDI GTCTC 1 cut(s) 563
BfaI CTAG 1 cut(s) 648
BfuAI ACCTGC 1 cut(s) 847
BisI GCNGC 4 cut(s) 9, 375, 378, 875
BlsI GCNGC 4 cut(s) 10, 376, 379, 876
Bme1390I CCNGG 1 cut(s) 859
Bme18I GGWCC 1 cut(s) 463
BmeRI GACNNNNNGTC 1 cut(s) 807
BmeT110I CYCGRG 1 cut(s) 275
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 1 cut(s) 463
BmiI GGNNCC 2 cut(s) 620, 640
BmrFI CCNGG 1 cut(s) 859
BmsI GCATC 4 cut(s) 323, 484, 586, 691
BoxI GACNNNNGTC 1 cut(s) 213
BplI GAGNNNNNCTC 2 cut(s) 258, 290
Bsa29I ATCGAT 1 cut(s) 186
BsaJI CCNNGG 4 cut(s) 114, 414, 699, 857
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 705
Bse1I ACTGG 4 cut(s) 344, 518, 625, 714
Bse3DI GCAATG 2 cut(s) 36, 820
BseBI CCWGG 1 cut(s) 859
BseCI ATCGAT 1 cut(s) 186
BseDI CCNNGG 4 cut(s) 114, 414, 699, 857
BseGI GGATG 5 cut(s) 222, 268, 508, 699, 911
BseLI CCNNNNNNNGG 2 cut(s) 101, 705
BseMI GCAATG 2 cut(s) 36, 820
BseMII CTCAG 2 cut(s) 332, 939
BseNI ACTGG 4 cut(s) 344, 518, 625, 714
BseRI GAGGAG 2 cut(s) 402, 453
BseSI GKGCMC 1 cut(s) 643
BseXI GCAGC 4 cut(s) 20, 386, 389, 886
BseYI CCCAGC 1 cut(s) 785
BsgI GTGCAG 1 cut(s) 893
Bsh1285I CGRYCG 1 cut(s) 556
BshFI GGCC 3 cut(s) 56, 287, 738
BshNI GGYRCC 2 cut(s) 618, 638
BshVI ATCGAT 1 cut(s) 186
BsiEI CGRYCG 1 cut(s) 556
BsiHKCI CYCGRG 1 cut(s) 275
BsiSI CCGG 1 cut(s) 762
BslI CCNNNNNNNGG 2 cut(s) 101, 705
BsmAI GTCTC 1 cut(s) 563
BsnI GGCC 3 cut(s) 56, 287, 738
BsoBI CYCGRG 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 643
Bsp143I GATC 5 cut(s) 354, 403, 660, 745, 853
BspACI CCGC 4 cut(s) 32, 63, 599, 643
BspANI GGCC 3 cut(s) 56, 287, 738
BspCNI CTCAG 2 cut(s) 331, 940
BspDI ATCGAT 1 cut(s) 186
BspLI GGNNCC 2 cut(s) 620, 640
BspMI ACCTGC 1 cut(s) 847
BspPI GGATC 3 cut(s) 668, 740, 848
BspT107I GGYRCC 2 cut(s) 618, 638
BsrDI GCAATG 2 cut(s) 36, 820
BsrI ACTGG 4 cut(s) 344, 518, 625, 714
BssECI CCNNGG 4 cut(s) 114, 414, 699, 857
BssMI GATC 5 cut(s) 354, 403, 660, 745, 853
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 1 cut(s) 859
Bst4CI ACNGT 2 cut(s) 365, 554
Bst6I CTCTTC 1 cut(s) 64
BstC8I GCNNGC 4 cut(s) 6, 525, 643, 740
BstDEI CTNAG 3 cut(s) 318, 467, 948
BstDSI CCRYGG 1 cut(s) 114
BstENI CCTNNNNNAGG 1 cut(s) 703
BstF5I GGATG 5 cut(s) 222, 268, 508, 699, 911
BstKTI GATC 5 cut(s) 357, 406, 663, 748, 856
BstMAI GTCTC 1 cut(s) 563
BstMBI GATC 5 cut(s) 354, 403, 660, 745, 853
BstMCI CGRYCG 1 cut(s) 556
BstMWI GCNNNNNNNGC 3 cut(s) 47, 62, 480
BstNI CCWGG 1 cut(s) 859
BstNSI RCATGY 1 cut(s) 531
BstPAI GACNNNNGTC 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 857
BstSLI GKGCMC 1 cut(s) 643
BstV1I GCAGC 4 cut(s) 20, 386, 389, 886
BstXI CCANNNNNNTGG 1 cut(s) 212
Bsu15I ATCGAT 1 cut(s) 186
BsuRI GGCC 3 cut(s) 56, 287, 738
BsuTUI ATCGAT 1 cut(s) 186
BtgI CCRYGG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 809
BtsCI GGATG 5 cut(s) 222, 268, 508, 699, 911
BtsI GCAGTG 2 cut(s) 504, 804
BtsIMutI CAGTG 2 cut(s) 504, 804
BveI ACCTGC 1 cut(s) 847
Cac8I GCNNGC 4 cut(s) 6, 525, 643, 740
Cfr13I GGNCC 1 cut(s) 463
ClaI ATCGAT 1 cut(s) 186
Csp6I GTAC 1 cut(s) 531
CviAII CATG 3 cut(s) 12, 174, 528
CviJI RGCY 6 cut(s) 56, 106, 280, 287, 419, 738
CviKI_1 RGCY 6 cut(s) 56, 106, 280, 287, 419, 738
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 3 cut(s) 318, 467, 948
DpnI GATC 5 cut(s) 356, 405, 662, 747, 855
DpnII GATC 5 cut(s) 354, 403, 660, 745, 853
DriI GACNNNNNGTC 1 cut(s) 807
EaeI YGGCCR 1 cut(s) 736
Eam1104I CTCTTC 1 cut(s) 64
Eam1105I GACNNNNNGTC 1 cut(s) 807
EarI CTCTTC 1 cut(s) 64
EciI GGCGGA 1 cut(s) 52
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 287
Eco32I GATATC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 463
Eco88I CYCGRG 1 cut(s) 275
EcoNI CCTNNNNNAGG 1 cut(s) 703
EcoRII CCWGG 1 cut(s) 857
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 699
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 3 cut(s) 15, 177, 531
FalI AAGNNNNNCTT 2 cut(s) 275, 307
FatI CATG 3 cut(s) 11, 173, 527
FauI CCCGC 2 cut(s) 39, 650
Fnu4HI GCNGC 4 cut(s) 9, 375, 378, 875
FokI GGATG 5 cut(s) 209, 275, 515, 706, 918
Fsp4HI GCNGC 4 cut(s) 9, 375, 378, 875
FspBI CTAG 1 cut(s) 648
GluI GCNGC 4 cut(s) 9, 375, 378, 875
GsaI CCCAGC 1 cut(s) 789
HaeIII GGCC 3 cut(s) 56, 287, 738
HapII CCGG 1 cut(s) 762
Hin1II CATG 3 cut(s) 15, 177, 531
HinfI GANTC 2 cut(s) 602, 910
HpaII CCGG 1 cut(s) 762
HphI GGTGA 1 cut(s) 194
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 5 cut(s) 100, 181, 354, 490, 949
Hpy188III TCNNGA 2 cut(s) 218, 557
Hpy8I GTNNAC 1 cut(s) 202
Hpy99I CGWCG 1 cut(s) 438
HpyCH4III ACNGT 2 cut(s) 365, 554
HpyCH4IV ACGT 1 cut(s) 808
HpyCH4V TGCA 7 cut(s) 4, 314, 360, 374, 497, 842, 874
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 62, 480
HpyF3I CTNAG 3 cut(s) 318, 467, 948
HpySE526I ACGT 1 cut(s) 808
Hsp92II CATG 3 cut(s) 15, 177, 531
Kzo9I GATC 5 cut(s) 354, 403, 660, 745, 853
Lsp1109I GCAGC 4 cut(s) 20, 386, 389, 886
LweI GCATC 4 cut(s) 323, 484, 586, 691
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 808
MaeIII GTNAC 3 cut(s) 118, 716, 799
MalI GATC 5 cut(s) 356, 405, 662, 747, 855
MboI GATC 5 cut(s) 354, 403, 660, 745, 853
MboII GAAGA 3 cut(s) 51, 718, 936
MhlI GDGCHC 1 cut(s) 643
MlsI TGGCCA 1 cut(s) 738
MluCI AATT 6 cut(s) 127, 192, 236, 408, 424, 537
MluNI TGGCCA 1 cut(s) 738
MmeI TCCRAC 1 cut(s) 359
Mox20I TGGCCA 1 cut(s) 738
MscI TGGCCA 1 cut(s) 738
MseI TTAA 1 cut(s) 423
MslI CAYNNNNRTG 1 cut(s) 41
Msp20I TGGCCA 1 cut(s) 738
MspI CCGG 1 cut(s) 762
MspR9I CCNGG 1 cut(s) 859
MvaI CCWGG 1 cut(s) 859
MwoI GCNNNNNNNGC 3 cut(s) 47, 62, 480
NdeII GATC 5 cut(s) 354, 403, 660, 745, 853
NlaIII CATG 3 cut(s) 15, 177, 531
NlaIV GGNNCC 2 cut(s) 620, 640
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 2 cut(s) 118, 799
NspI RCATGY 1 cut(s) 531
PaeR7I CTCGAG 1 cut(s) 275
PceI AGGCCT 1 cut(s) 287
PfeI GAWTC 2 cut(s) 602, 910
PflMI CCANNNNNTGG 1 cut(s) 101
PkrI GCNGC 4 cut(s) 10, 376, 379, 876
PshAI GACNNNNGTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 857
PspFI CCCAGC 1 cut(s) 785
PspGI CCWGG 1 cut(s) 857
PspN4I GGNNCC 2 cut(s) 620, 640
PspPI GGNCC 1 cut(s) 463
PspXI VCTCGAGB 1 cut(s) 275
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 1 cut(s) 423
SatI GCNGC 4 cut(s) 9, 375, 378, 875
Sau3AI GATC 5 cut(s) 354, 403, 660, 745, 853
Sau96I GGNCC 1 cut(s) 463
ScrFI CCNGG 1 cut(s) 859
SduI GDGCHC 1 cut(s) 643
SfaNI GCATC 4 cut(s) 323, 484, 586, 691
Sfr274I CTCGAG 1 cut(s) 275
SinI GGWCC 1 cut(s) 463
SlaI CTCGAG 1 cut(s) 275
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 6 cut(s) 127, 192, 236, 408, 424, 537
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 4 cut(s) 32, 63, 599, 643
SspMI CTAG 1 cut(s) 648
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 857
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 2 cut(s) 365, 554
TaiI ACGT 1 cut(s) 811
TaqI TCGA 5 cut(s) 186, 276, 406, 436, 556
TasI AATT 6 cut(s) 127, 192, 236, 408, 424, 537
TatI WGTACW 1 cut(s) 530
TfiI GAWTC 2 cut(s) 602, 910
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 2 cut(s) 504, 804
TseFI GTSAC 2 cut(s) 118, 799
TseI GCWGC 4 cut(s) 8, 374, 377, 874
Tsp45I GTSAC 2 cut(s) 118, 799
TspDTI ATGAA 3 cut(s) 98, 285, 289
TspGWI ACGGA 3 cut(s) 103, 245, 578
TspRI CASTG 2 cut(s) 504, 804
Van91I CCANNNNNTGG 1 cut(s) 101
VpaK11BI GGWCC 1 cut(s) 463
XagI CCTNNNNNAGG 1 cut(s) 703
XapI RAATTY 2 cut(s) 236, 537
XceI RCATGY 1 cut(s) 531
XhoI CTCGAG 1 cut(s) 275
XspI CTAG 1 cut(s) 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.