RLG00000034817

Lysosomal Pro-X

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017520
Physical Location & Seq
Reverse (-)
55081255 .. 55083650
2396 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034817

Sequence Viewer

Length: 1563 bp
ATGGCCAGCACAGCTCCAAGAATCCAAGCTCTTGACCTTCCATGGCTACTACTACTTTTTTTCGTCTTTTCAACCTTCATCACCAGTTCTTGCTCTTCTCCCTGGAGAAAAATACCGAGGCTCCGCATCCTACAAAGACCTATAACTTTTCGAAATCACAGCACTTCTGTTGCTGCCCGCCACCATTTCAGGACTTACTATTATGCTCAAACGCTAGACCACTTCAACTACTGGCCAGGGAGTTATGCCACCTTTCTGCAGCGTTACATGGTAAGTTCTATGCATTGGAGAGGTGCCGGTGCAGCCGCTCCTATGTTCGTGTACCTTGGCGGGGAGGAGTCCTTGGACGAAAGTATTGGCTCCATCGGATTTCTCACCGATAATGCGCCGAAATTTGGTGCTTTAATGGTATATATAGAGCATCGATACTATGGGAAATCAATTCCATCTAGACCGAACATGCAGGATATATACAACGCTACCAGCCTCGGATATCTGAACTCCGCGCAAGCTCTGGCCGATTATGCAGAAATTATACTACACTTGAAGAGGAATTTATCGGCCGATGCTTCTCCAGTAATTGTCATTGGAGGCTCTTATGGGGGAATGCTTGCAGCATGGTTTCGCCTCAAATACCCGCACATTGCTGTGGGGGCACTGGCCTCTTCCGCCCCACTGATTTACTTTGATGACATTACACCATCAGATGGGCTTCATTCCGTGGTCACAAACAATTTCAGGGAAGCGAGTGAGAACTGTTACAATACCATAAAGAAATCATGGTCTGAAATTGATAGAATTGCTGTTCACCAAGACGATGGTCTTGACATCCTCACTGAGAAATTTCAAACTTGCGAACCTCTGGAGGATGGAGATGAACTCGAGCTGAAGGCCTACTTGGAGTTCATATATATAACTGCATCTCAGTATGATGACCCTCCAACATATCCAGTCAATCAGATCTGCAACAGTATAAATGCAGCAGCAGACAGAGGAGATGATATCATTGATCGAATTGCCTCGGCTGTTAATTATACTCGTCGAGGAGAGAATAGAGGTCAATGCTTGGACCTAAGTAGCGAACTTCGCCCATCAGATGATGCAGTGGATGGTTTGAACTGGTTATGGCAGGCATGTACTGAAATTTTTATGCCAATCGGTCGAGAAACAAACGAGACTATGTTCCAGGCAGAACCATTTGATGCGGATTCCTACACTCAAAGGTGCCACCAGTTATATCAAGGTGCCCGCCCTAGACCCCATTGGATCACAACTTATTATGGGGGGCATCATATAAGGATGACCTTGGAGAGGTTTGCCAGTAACATCATCTTCTCAAATGGCCAGCGTGATCCTTTTAGCGTTGCCGGGGTATTGACAAATATATCGCCCAGCATAGTAGCCGTGACTACACGACAAGGTTCTCATTGCTTGGACTTATTACCTGCAAGGATAAACGATCCCAGATGGTTAGTTGTGCAGCGACACATAGAAGTTGGGATTATCACAGGATGGATTCGCAACTACTACAATGATTTGCGTATCTTTTCTTCTGAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04614 ko04974 map04614 map04974
Pfam Domains
Protein Families
Peptidase_S28

Protein Analysis

521

Amino Acids

58.93

Weight (kDa)

5.89

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S28 PF05577 70 - 496 1.1e-81 Serine carboxypeptidase S28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017520)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28680
fragaria_vesca FvH4_3g28620
rosa_chinensis RchiOBHm_Chr5g0052461 RchiOBHm_Chr5g0052471
rosa_laevigata RLG00000034817
rosa_multiflora Rmu_sc0006803.1_g000044
rosa_roxburghii Rroxscaffold_1G00028350
rosa_rugosa Rorug05G0271800
rosa_samantha Rh5BG355500 Rh5CG380900 Rh5DG370700
rosa_wichuraiana Rw5G032520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1453
AccB1I GGYRCC 3 cut(s) 293, 1224, 1244
AccB7I CCANNNNNTGG 1 cut(s) 707
AccBSI CCGCTC 1 cut(s) 308
AccII CGCG 1 cut(s) 506
AciI CCGC 9 cut(s) 124, 178, 306, 330, 504, 638, 669, 1205, 1249
AclWI GGATC 3 cut(s) 1274, 1346, 1454
AcoI YGGCCR 5 cut(s) 3, 233, 516, 561, 1342
AcsI RAATTY 4 cut(s) 392, 553, 842, 1143
AcuI CTGAAG 1 cut(s) 908
AfaI GTAC 2 cut(s) 323, 1138
AfiI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1311
AgsI TTSAA 5 cut(s) 72, 226, 547, 848, 1117
AjnI CCWGG 3 cut(s) 101, 235, 1185
AjuI GAANNNNNNNTTGG 2 cut(s) 881, 913
AluBI AGCT 4 cut(s) 14, 29, 512, 886
AluI AGCT 4 cut(s) 14, 29, 512, 886
Alw26I GTCTC 1 cut(s) 1169
AlwI GGATC 3 cut(s) 1274, 1346, 1454
Ama87I CYCGRG 1 cut(s) 881
AoxI GGCC 7 cut(s) 3, 233, 516, 561, 660, 891, 1342
ApeKI GCWGC 7 cut(s) 173, 259, 302, 614, 980, 983, 1480
ApoI RAATTY 4 cut(s) 392, 553, 842, 1143
AspLEI GCGC 2 cut(s) 388, 508
AspS9I GGNCC 1 cut(s) 1069
AsuC2I CCSGG 1 cut(s) 1369
AsuHPI GGTGA 3 cut(s) 73, 367, 800
AsuII TTCGAA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 881
AvaII GGWCC 1 cut(s) 1069
BaeGI GKGCMC 2 cut(s) 658, 1249
BalI TGGCCA 3 cut(s) 5, 235, 1344
BanI GGYRCC 3 cut(s) 293, 1224, 1244
BarI GAAGNNNNNNTAC 1 cut(s) 1534
BbvI GCAGC 7 cut(s) 160, 271, 314, 626, 992, 995, 1492
BceAI ACGGC 1 cut(s) 1388
BciT130I CCWGG 3 cut(s) 103, 237, 1187
BcnI CCSGG 1 cut(s) 1369
BcoDI GTCTC 1 cut(s) 1169
BfaI CTAG 3 cut(s) 215, 450, 1254
BfmI CTRYAG 1 cut(s) 257
BfuAI ACCTGC 1 cut(s) 1453
BglII AGATCT 1 cut(s) 960
BisI GCNGC 8 cut(s) 174, 260, 303, 306, 615, 981, 984, 1481
BlsI GCNGC 8 cut(s) 175, 261, 304, 307, 616, 982, 985, 1482
Bme1390I CCNGG 4 cut(s) 103, 237, 1187, 1369
Bme18I GGWCC 1 cut(s) 1069
BmeT110I CYCGRG 1 cut(s) 881
BmgT120I GGNCC 1 cut(s) 1069
BmiI GGNNCC 5 cut(s) 122, 295, 361, 1226, 1246
BmrFI CCNGG 4 cut(s) 103, 237, 1187, 1369
BmsI GCATC 7 cut(s) 135, 430, 556, 929, 1090, 1192, 1297
BoxI GACNNNNGTC 1 cut(s) 819
BplI GAGNNNNNCTC 2 cut(s) 864, 896
BpmI CTGGAG 3 cut(s) 124, 558, 884
Bpu14I TTCGAA 1 cut(s) 151
BpuMI CCSGG 1 cut(s) 1369
Bsa29I ATCGAT 1 cut(s) 424
BsaXI ACNNNNNCTCC 2 cut(s) 105, 135
Bsc4I CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1311
Bse118I RCCGGY 1 cut(s) 296
Bse1I ACTGG 8 cut(s) 84, 236, 575, 663, 950, 1124, 1231, 1320
Bse3DI GCAATG 2 cut(s) 642, 1426
BseBI CCWGG 3 cut(s) 103, 237, 1187
BseCI ATCGAT 1 cut(s) 424
BseGI GGATG 6 cut(s) 126, 828, 874, 1114, 1305, 1517
BseLI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1311
BseMI GCAATG 2 cut(s) 642, 1426
BseMII CTCAG 3 cut(s) 828, 938, 1545
BseNI ACTGG 8 cut(s) 84, 236, 575, 663, 950, 1124, 1231, 1320
BseRI GAGGAG 3 cut(s) 350, 1008, 1059
BseSI GKGCMC 2 cut(s) 658, 1249
BseX3I CGGCCG 1 cut(s) 561
BseXI GCAGC 7 cut(s) 160, 271, 314, 626, 992, 995, 1492
BseYI CCCAGC 1 cut(s) 1391
BsgI GTGCAG 2 cut(s) 321, 1499
Bsh1236I CGCG 1 cut(s) 506
Bsh1285I CGRYCG 2 cut(s) 564, 1162
BshFI GGCC 7 cut(s) 5, 235, 518, 563, 662, 893, 1344
BshNI GGYRCC 3 cut(s) 293, 1224, 1244
BshVI ATCGAT 1 cut(s) 424
BsiEI CGRYCG 2 cut(s) 564, 1162
BsiHKCI CYCGRG 1 cut(s) 881
BsiSI CCGG 2 cut(s) 297, 1368
BslI CCNNNNNNNGG 4 cut(s) 331, 395, 707, 1311
BsmAI GTCTC 1 cut(s) 1169
BsmI GAATGC 1 cut(s) 612
BsnI GGCC 7 cut(s) 5, 235, 518, 563, 662, 893, 1344
BsoBI CYCGRG 1 cut(s) 881
Bsp119I TTCGAA 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 658, 1249
Bsp143I GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
Bsp19I CCATGG 1 cut(s) 41
BspACI CCGC 9 cut(s) 124, 178, 306, 330, 504, 638, 669, 1205, 1249
BspANI GGCC 7 cut(s) 5, 235, 518, 563, 662, 893, 1344
BspCNI CTCAG 3 cut(s) 829, 937, 1546
BspDI ATCGAT 1 cut(s) 424
BspFNI CGCG 1 cut(s) 506
BspLI GGNNCC 5 cut(s) 122, 295, 361, 1226, 1246
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 1453
BspPI GGATC 3 cut(s) 1274, 1346, 1454
BspQI GCTCTTC 1 cut(s) 100
BspT104I TTCGAA 1 cut(s) 151
BspT107I GGYRCC 3 cut(s) 293, 1224, 1244
BsrBI CCGCTC 1 cut(s) 308
BsrDI GCAATG 2 cut(s) 642, 1426
BsrFI RCCGGY 1 cut(s) 296
BsrI ACTGG 8 cut(s) 84, 236, 575, 663, 950, 1124, 1231, 1320
BssAI RCCGGY 1 cut(s) 296
BssMI GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
BssT1I CCWWGG 4 cut(s) 41, 325, 342, 1305
Bst2UI CCWGG 3 cut(s) 103, 237, 1187
Bst4CI ACNGT 2 cut(s) 758, 971
Bst6I CTCTTC 3 cut(s) 100, 542, 670
BstBI TTCGAA 1 cut(s) 151
BstC8I GCNNGC 7 cut(s) 7, 178, 510, 612, 1131, 1249, 1346
BstDEI CTNAG 4 cut(s) 837, 924, 1073, 1554
BstDSI CCRYGG 2 cut(s) 41, 720
BstENI CCTNNNNNAGG 1 cut(s) 1309
BstF5I GGATG 6 cut(s) 126, 828, 874, 1114, 1305, 1517
BstFNI CGCG 1 cut(s) 506
BstHHI GCGC 2 cut(s) 388, 508
BstKTI GATC 5 cut(s) 963, 1012, 1269, 1354, 1462
BstMAI GTCTC 1 cut(s) 1169
BstMBI GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
BstMCI CGRYCG 2 cut(s) 564, 1162
BstMWI GCNNNNNNNGC 6 cut(s) 11, 302, 524, 653, 668, 1086
BstNI CCWGG 3 cut(s) 103, 237, 1187
BstNSI RCATGY 2 cut(s) 463, 1137
BstPAI GACNNNNGTC 1 cut(s) 819
BstSCI CCNGG 4 cut(s) 101, 235, 1185, 1367
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 2 cut(s) 658, 1249
BstUI CGCG 1 cut(s) 506
BstV1I GCAGC 7 cut(s) 160, 271, 314, 626, 992, 995, 1492
BstX2I RGATCY 1 cut(s) 960
BstXI CCANNNNNNTGG 1 cut(s) 818
BstYI RGATCY 1 cut(s) 960
BstZI CGGCCG 1 cut(s) 561
Bsu15I ATCGAT 1 cut(s) 424
BsuRI GGCC 7 cut(s) 5, 235, 518, 563, 662, 893, 1344
BsuTUI ATCGAT 1 cut(s) 424
BtgI CCRYGG 2 cut(s) 41, 720
BtsCI GGATG 6 cut(s) 126, 828, 874, 1114, 1305, 1517
BtsI GCAGTG 1 cut(s) 1110
BtsIMutI CAGTG 4 cut(s) 656, 674, 834, 1110
BveI ACCTGC 1 cut(s) 1453
Cac8I GCNNGC 7 cut(s) 7, 178, 510, 612, 1131, 1249, 1346
CfoI GCGC 2 cut(s) 388, 508
Cfr10I RCCGGY 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 1069
ClaI ATCGAT 1 cut(s) 424
Csp6I GTAC 2 cut(s) 322, 1137
CviAII CATG 6 cut(s) 42, 268, 460, 618, 780, 1134
CviQI GTAC 2 cut(s) 322, 1137
DdeI CTNAG 4 cut(s) 837, 924, 1073, 1554
DpnI GATC 5 cut(s) 962, 1011, 1268, 1353, 1461
DpnII GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
EaeI YGGCCR 5 cut(s) 3, 233, 516, 561, 1342
EagI CGGCCG 1 cut(s) 561
Eam1104I CTCTTC 3 cut(s) 100, 542, 670
EarI CTCTTC 3 cut(s) 100, 542, 670
EciI GGCGGA 1 cut(s) 658
EclXI CGGCCG 1 cut(s) 561
Eco130I CCWWGG 4 cut(s) 41, 325, 342, 1305
Eco147I AGGCCT 1 cut(s) 893
Eco32I GATATC 2 cut(s) 494, 1003
Eco47I GGWCC 1 cut(s) 1069
Eco52I CGGCCG 1 cut(s) 561
Eco57I CTGAAG 1 cut(s) 908
Eco88I CYCGRG 1 cut(s) 881
EcoNI CCTNNNNNAGG 1 cut(s) 1309
EcoRII CCWGG 3 cut(s) 101, 235, 1185
EcoRV GATATC 2 cut(s) 494, 1003
EcoT14I CCWWGG 4 cut(s) 41, 325, 342, 1305
EcoT22I ATGCAT 1 cut(s) 285
ErhI CCWWGG 4 cut(s) 41, 325, 342, 1305
FaeI CATG 6 cut(s) 45, 271, 463, 621, 783, 1137
FalI AAGNNNNNCTT 2 cut(s) 881, 913
FatI CATG 6 cut(s) 41, 267, 459, 617, 779, 1133
FauI CCCGC 4 cut(s) 185, 323, 645, 1256
Fnu4HI GCNGC 8 cut(s) 174, 260, 303, 306, 615, 981, 984, 1481
FokI GGATG 6 cut(s) 113, 815, 881, 1121, 1312, 1524
Fsp4HI GCNGC 8 cut(s) 174, 260, 303, 306, 615, 981, 984, 1481
FspBI CTAG 3 cut(s) 215, 450, 1254
GlaI GCGC 2 cut(s) 387, 507
GluI GCNGC 8 cut(s) 174, 260, 303, 306, 615, 981, 984, 1481
GsaI CCCAGC 1 cut(s) 1395
GsuI CTGGAG 3 cut(s) 124, 558, 884
HaeIII GGCC 7 cut(s) 5, 235, 518, 563, 662, 893, 1344
HapII CCGG 2 cut(s) 297, 1368
HhaI GCGC 2 cut(s) 388, 508
Hin1II CATG 6 cut(s) 45, 271, 463, 621, 783, 1137
Hin6I GCGC 2 cut(s) 386, 506
HinP1I GCGC 2 cut(s) 386, 506
HinfI GANTC 4 cut(s) 21, 338, 1208, 1516
HpaII CCGG 2 cut(s) 297, 1368
HphI GGTGA 3 cut(s) 73, 367, 800
Hpy166II GTNNAC 2 cut(s) 322, 808
Hpy188I TCNGA 8 cut(s) 368, 491, 498, 706, 787, 960, 1096, 1555
Hpy188III TCNNGA 6 cut(s) 32, 190, 450, 824, 863, 1163
Hpy8I GTNNAC 2 cut(s) 322, 808
Hpy99I CGWCG 1 cut(s) 1044
HpyAV CCTTC 3 cut(s) 47, 85, 883
HpyCH4III ACNGT 2 cut(s) 758, 971
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 302, 524, 653, 668, 1086
HpyF3I CTNAG 4 cut(s) 837, 924, 1073, 1554
Hsp92II CATG 6 cut(s) 45, 271, 463, 621, 783, 1137
HspAI GCGC 2 cut(s) 386, 506
Kzo9I GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
LguI GCTCTTC 1 cut(s) 100
LmnI GCTCC 4 cut(s) 19, 126, 313, 365
Lsp1109I GCAGC 7 cut(s) 160, 271, 314, 626, 992, 995, 1492
LweI GCATC 7 cut(s) 135, 430, 556, 929, 1090, 1192, 1297
MaeI CTAG 3 cut(s) 215, 450, 1254
MaeIII GTNAC 5 cut(s) 263, 724, 758, 1322, 1405
MalI GATC 5 cut(s) 962, 1011, 1268, 1353, 1461
MbiI CCGCTC 1 cut(s) 308
MboI GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
MboII GAAGA 5 cut(s) 87, 559, 657, 1324, 1542
MflI RGATCY 1 cut(s) 960
MhlI GDGCHC 2 cut(s) 658, 1249
MlsI TGGCCA 3 cut(s) 5, 235, 1344
MluNI TGGCCA 3 cut(s) 5, 235, 1344
MlyI GAGTC 1 cut(s) 347
MmeI TCCRAC 1 cut(s) 965
Mox20I TGGCCA 3 cut(s) 5, 235, 1344
Mph1103I ATGCAT 1 cut(s) 285
MscI TGGCCA 3 cut(s) 5, 235, 1344
MseI TTAA 2 cut(s) 404, 1029
MslI CAYNNNNRTG 1 cut(s) 647
Msp20I TGGCCA 3 cut(s) 5, 235, 1344
MspI CCGG 2 cut(s) 297, 1368
MspR9I CCNGG 4 cut(s) 103, 237, 1187, 1369
Mva1269I GAATGC 1 cut(s) 612
MvaI CCWGG 3 cut(s) 103, 237, 1187
MvnI CGCG 1 cut(s) 506
MwoI GCNNNNNNNGC 6 cut(s) 11, 302, 524, 653, 668, 1086
NciI CCSGG 1 cut(s) 1369
NcoI CCATGG 1 cut(s) 41
NdeII GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
NlaIII CATG 6 cut(s) 45, 271, 463, 621, 783, 1137
NlaIV GGNNCC 5 cut(s) 122, 295, 361, 1226, 1246
NmeAIII GCCGAG 1 cut(s) 1001
NmuCI GTSAC 2 cut(s) 724, 1405
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 2 cut(s) 463, 1137
NspV TTCGAA 1 cut(s) 151
PaeR7I CTCGAG 1 cut(s) 881
PceI AGGCCT 1 cut(s) 893
PciSI GCTCTTC 1 cut(s) 100
PctI GAATGC 1 cut(s) 612
PfeI GAWTC 3 cut(s) 21, 1208, 1516
PflMI CCANNNNNTGG 1 cut(s) 707
PkrI GCNGC 8 cut(s) 175, 261, 304, 307, 616, 982, 985, 1482
PleI GAGTC 1 cut(s) 346
PpsI GAGTC 1 cut(s) 346
PshAI GACNNNNGTC 1 cut(s) 819
Psp6I CCWGG 3 cut(s) 101, 235, 1185
PspFI CCCAGC 1 cut(s) 1391
PspGI CCWGG 3 cut(s) 101, 235, 1185
PspN4I GGNNCC 5 cut(s) 122, 295, 361, 1226, 1246
PspPI GGNCC 1 cut(s) 1069
PspXI VCTCGAGB 1 cut(s) 881
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 960
RsaI GTAC 2 cut(s) 323, 1138
RsaNI GTAC 2 cut(s) 322, 1137
RseI CAYNNNNRTG 1 cut(s) 647
SapI GCTCTTC 1 cut(s) 100
SaqAI TTAA 2 cut(s) 404, 1029
SatI GCNGC 8 cut(s) 174, 260, 303, 306, 615, 981, 984, 1481
Sau3AI GATC 5 cut(s) 960, 1009, 1266, 1351, 1459
Sau96I GGNCC 1 cut(s) 1069
SchI GAGTC 1 cut(s) 347
ScrFI CCNGG 4 cut(s) 103, 237, 1187, 1369
SduI GDGCHC 2 cut(s) 658, 1249
SfaNI GCATC 7 cut(s) 135, 430, 556, 929, 1090, 1192, 1297
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 1 cut(s) 881
SfuI TTCGAA 1 cut(s) 151
SinI GGWCC 1 cut(s) 1069
SlaI CTCGAG 1 cut(s) 881
SmiMI CAYNNNNRTG 1 cut(s) 647
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SseBI AGGCCT 1 cut(s) 893
SsiI CCGC 9 cut(s) 124, 178, 306, 330, 504, 638, 669, 1205, 1249
SspMI CTAG 3 cut(s) 215, 450, 1254
StuI AGGCCT 1 cut(s) 893
StyD4I CCNGG 4 cut(s) 101, 235, 1185, 1367
StyI CCWWGG 4 cut(s) 41, 325, 342, 1305
TaaI ACNGT 2 cut(s) 758, 971
TaqI TCGA 6 cut(s) 151, 424, 882, 1012, 1042, 1162
TaqII GACCGA 2 cut(s) 469, 1148
TatI WGTACW 1 cut(s) 1136
TauI GCSGC 1 cut(s) 308
TfiI GAWTC 3 cut(s) 21, 1208, 1516
Tru1I TTAA 2 cut(s) 404, 1029
Tru9I TTAA 2 cut(s) 404, 1029
TscAI CASTG 4 cut(s) 663, 681, 841, 1110
TseFI GTSAC 2 cut(s) 724, 1405
TseI GCWGC 7 cut(s) 173, 259, 302, 614, 980, 983, 1480
Tsp45I GTSAC 2 cut(s) 724, 1405
TspDTI ATGAA 4 cut(s) 67, 704, 891, 895
TspGWI ACGGA 1 cut(s) 709
TspRI CASTG 4 cut(s) 663, 681, 841, 1110
Van91I CCANNNNNTGG 1 cut(s) 707
VpaK11BI GGWCC 1 cut(s) 1069
XagI CCTNNNNNAGG 1 cut(s) 1309
XapI RAATTY 4 cut(s) 392, 553, 842, 1143
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 2 cut(s) 463, 1137
XhoI CTCGAG 1 cut(s) 881
XspI CTAG 3 cut(s) 215, 450, 1254
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.