FvH4_3g29930

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010479
Physical Location & Seq
Forward (+)
23032116 .. 23033949
1834 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29930.t1

Sequence Viewer

Length: 603 bp
ATGCCGAGTCCGAAGAGCCAGCGAAGGGTGCAAGAGGAGAGCAAGAACCGGAGGGGCAGATTAGTCTCGGAGAGATCTTTGTCGTTTCACGGCCAGACGTTGGCCGCGACGACGCCGCAGCTCCGGCGGCCGAAGACGGTCCCCGACATGGTCATGTATAGGACAGCAGTCGGAGCCACGACGCCTCCGACGGCGGCGCAGGAGCTTCGGCCGAAGCTGACGAAGCTGCTGCTCAACGTGACTATACAGGGGAGCGTCGGTGCCGTTCAGGTGGTGATGTCGCCGGAATCTACGGTGGGTGATCTGGTGGCGGCAGCCGTAAGGCAGTACGAGAAAGAAGGCCGCCGGCCGATCTTTCCGTCCGTTGATCCGTCACGGTTCGACCTCCATTATTCGCAGTTCAGTTTAGAAAGTTTGGATAGGGAGGAGAAGCTGATGACGTTGGGGTCTCGCAACTTTTTCCTGTGCCCTAAGAAGTCGGCAGTTGACGGTGAAAGTTGCGGTGGTGGGATAACGACGACGGCTGGTTCATGTTCGAAACAGGCGGAGAAAGCTTCCAAAACTGGGTTTTCATGGCTCAAGTTTATGGATTCTCTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

21.95

Weight (kDa)

9.81

Isoelectric Point (pI)

60.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 72 - 156 7.9e-39 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 445
AccB1I GGYRCC 1 cut(s) 260
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 107
AciI CCGC 8 cut(s) 105, 116, 127, 194, 311, 343, 501, 545
AclWI GGATC 1 cut(s) 362
AcoI YGGCCR 5 cut(s) 91, 102, 128, 209, 347
AcyI GRCGYC 2 cut(s) 113, 182
AfaI GTAC 1 cut(s) 329
AfiI CCNNNNNNNGG 4 cut(s) 25, 100, 148, 564
AluBI AGCT 6 cut(s) 121, 205, 217, 226, 433, 554
AluI AGCT 6 cut(s) 121, 205, 217, 226, 433, 554
Alw26I GTCTC 2 cut(s) 70, 453
AlwI GGATC 1 cut(s) 362
AoxI GGCC 6 cut(s) 91, 102, 128, 209, 340, 347
ApeKI GCWGC 4 cut(s) 118, 226, 229, 314
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 1 cut(s) 139
AsuHPI GGTGA 3 cut(s) 286, 311, 503
AsuII TTCGAA 1 cut(s) 536
AvaII GGWCC 1 cut(s) 139
BaeGI GKGCMC 1 cut(s) 470
BanI GGYRCC 1 cut(s) 260
BbsI GAAGAC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 130, 213, 216, 326
BceAI ACGGC 5 cut(s) 106, 207, 248, 302, 537
BcgI CGANNNNNNTGC 4 cut(s) 188, 211, 222, 245
BcoDI GTCTC 2 cut(s) 70, 453
BglII AGATCT 1 cut(s) 74
Bme18I GGWCC 1 cut(s) 139
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 3 cut(s) 141, 175, 262
BmrI ACTGGG 1 cut(s) 573
BmuI ACTGGG 1 cut(s) 573
BoxI GACNNNNGTC 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 140
Bpu14I TTCGAA 1 cut(s) 536
BpuEI CTTGAG 1 cut(s) 563
BsaHI GRCGYC 2 cut(s) 113, 182
BsaI GGTCTC 1 cut(s) 453
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 4 cut(s) 169, 199, 244, 274
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 100, 148, 564
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 1 cut(s) 568
BseLI CCNNNNNNNGG 4 cut(s) 25, 100, 148, 564
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 2 cut(s) 50, 440
BseSI GKGCMC 1 cut(s) 470
BseX3I CGGCCG 3 cut(s) 128, 209, 347
BseXI GCAGC 4 cut(s) 130, 213, 216, 326
Bsh1236I CGCG 1 cut(s) 107
Bsh1285I CGRYCG 3 cut(s) 131, 212, 350
BshFI GGCC 6 cut(s) 93, 104, 130, 211, 342, 349
BshNI GGYRCC 1 cut(s) 260
BsiEI CGRYCG 3 cut(s) 131, 212, 350
BsiSI CCGG 4 cut(s) 49, 124, 284, 346
BslFI GGGAC 1 cut(s) 125
BslI CCNNNNNNNGG 4 cut(s) 25, 100, 148, 564
BsmAI GTCTC 2 cut(s) 70, 453
BsmFI GGGAC 1 cut(s) 125
BsnI GGCC 6 cut(s) 93, 104, 130, 211, 342, 349
Bso31I GGTCTC 1 cut(s) 453
Bsp119I TTCGAA 1 cut(s) 536
Bsp1286I GDGCHC 1 cut(s) 470
Bsp143I GATC 4 cut(s) 74, 301, 351, 367
BspACI CCGC 8 cut(s) 105, 116, 127, 194, 311, 343, 501, 545
BspANI GGCC 6 cut(s) 93, 104, 130, 211, 342, 349
BspFNI CGCG 1 cut(s) 107
BspLI GGNNCC 3 cut(s) 141, 175, 262
BspPI GGATC 1 cut(s) 362
BspQI GCTCTTC 1 cut(s) 8
BspT104I TTCGAA 1 cut(s) 536
BspT107I GGYRCC 1 cut(s) 260
BspTNI GGTCTC 1 cut(s) 453
BsrFI RCCGGY 1 cut(s) 345
BsrI ACTGG 1 cut(s) 568
BssAI RCCGGY 1 cut(s) 345
BssMI GATC 4 cut(s) 74, 301, 351, 367
BssNI GRCGYC 2 cut(s) 113, 182
Bst4CI ACNGT 4 cut(s) 139, 295, 378, 491
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 2 cut(s) 113, 182
BstBI TTCGAA 1 cut(s) 536
BstC8I GCNNGC 2 cut(s) 20, 347
BstDEI CTNAG 1 cut(s) 471
BstFNI CGCG 1 cut(s) 107
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 4 cut(s) 77, 304, 354, 370
BstMAI GTCTC 2 cut(s) 70, 453
BstMBI GATC 4 cut(s) 74, 301, 351, 367
BstMCI CGRYCG 3 cut(s) 131, 212, 350
BstMWI GCNNNNNNNGC 6 cut(s) 28, 124, 127, 173, 223, 551
BstPAI GACNNNNGTC 1 cut(s) 167
BstSLI GKGCMC 1 cut(s) 470
BstUI CGCG 1 cut(s) 107
BstV1I GCAGC 4 cut(s) 130, 213, 216, 326
BstV2I GAAGAC 1 cut(s) 140
BstX2I RGATCY 1 cut(s) 74
BstYI RGATCY 1 cut(s) 74
BstZI CGGCCG 3 cut(s) 128, 209, 347
BsuRI GGCC 6 cut(s) 93, 104, 130, 211, 342, 349
Cac8I GCNNGC 2 cut(s) 20, 347
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 345
Cfr13I GGNCC 1 cut(s) 139
CseI GACGC 3 cut(s) 121, 190, 244
Csp6I GTAC 1 cut(s) 328
CviAII CATG 4 cut(s) 148, 154, 531, 573
CviQI GTAC 1 cut(s) 328
DdeI CTNAG 1 cut(s) 471
DpnI GATC 4 cut(s) 76, 303, 353, 369
DpnII GATC 4 cut(s) 74, 301, 351, 367
DrdI GACNNNNNNGTC 1 cut(s) 445
DseDI GACNNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 5 cut(s) 91, 102, 128, 209, 347
EagI CGGCCG 3 cut(s) 128, 209, 347
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 560
EclXI CGGCCG 3 cut(s) 128, 209, 347
Eco31I GGTCTC 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 139
Eco52I CGGCCG 3 cut(s) 128, 209, 347
FaeI CATG 4 cut(s) 151, 157, 534, 576
FaiI YATR 7 cut(s) 149, 155, 159, 245, 532, 574, 587
FaqI GGGAC 1 cut(s) 125
FatI CATG 4 cut(s) 147, 153, 530, 572
GlaI GCGC 1 cut(s) 198
HaeIII GGCC 6 cut(s) 93, 104, 130, 211, 342, 349
HapII CCGG 4 cut(s) 49, 124, 284, 346
HgaI GACGC 3 cut(s) 121, 190, 244
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 2 cut(s) 113, 182
Hin1II CATG 4 cut(s) 151, 157, 534, 576
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HincII GTYRAC 1 cut(s) 487
HindII GTYRAC 1 cut(s) 487
HindIII AAGCTT 1 cut(s) 552
HinfI GANTC 3 cut(s) 7, 287, 590
HpaII CCGG 4 cut(s) 49, 124, 284, 346
HphI GGTGA 3 cut(s) 286, 311, 503
Hpy166II GTNNAC 1 cut(s) 487
Hpy188I TCNGA 4 cut(s) 12, 70, 173, 189
Hpy8I GTNNAC 1 cut(s) 487
Hpy99I CGWCG 7 cut(s) 112, 115, 184, 193, 260, 520, 523
HpyAV CCTTC 2 cut(s) 18, 332
HpyCH4III ACNGT 4 cut(s) 139, 295, 378, 491
HpyCH4IV ACGT 3 cut(s) 98, 237, 440
HpyCH4V TGCA 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 124, 127, 173, 223, 551
HpyF3I CTNAG 1 cut(s) 471
HpySE526I ACGT 3 cut(s) 98, 237, 440
Hsp92I GRCGYC 2 cut(s) 113, 182
Hsp92II CATG 4 cut(s) 151, 157, 534, 576
HspAI GCGC 1 cut(s) 197
KroI GCCGGC 1 cut(s) 345
KroNI GCCGGC 1 cut(s) 347
Kzo9I GATC 4 cut(s) 74, 301, 351, 367
LguI GCTCTTC 1 cut(s) 8
LmnI GCTCC 4 cut(s) 126, 173, 202, 252
Lsp1109I GCAGC 4 cut(s) 130, 213, 216, 326
MaeII ACGT 3 cut(s) 98, 237, 440
MaeIII GTNAC 2 cut(s) 238, 372
MalI GATC 4 cut(s) 76, 303, 353, 369
MboI GATC 4 cut(s) 74, 301, 351, 367
MboII GAAGA 2 cut(s) 25, 145
MflI RGATCY 1 cut(s) 74
MhlI GDGCHC 1 cut(s) 470
MlyI GAGTC 1 cut(s) 16
MmeI TCCRAC 2 cut(s) 151, 212
MnlI CCTC 5 cut(s) 28, 45, 195, 395, 418
MroNI GCCGGC 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 152
MspI CCGG 4 cut(s) 49, 124, 284, 346
MvnI CGCG 1 cut(s) 107
MwoI GCNNNNNNNGC 6 cut(s) 28, 124, 127, 173, 223, 551
NaeI GCCGGC 1 cut(s) 347
NdeII GATC 4 cut(s) 74, 301, 351, 367
NgoMIV GCCGGC 1 cut(s) 345
NlaIII CATG 4 cut(s) 151, 157, 534, 576
NlaIV GGNNCC 3 cut(s) 141, 175, 262
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 238, 372
NspV TTCGAA 1 cut(s) 536
PciSI GCTCTTC 1 cut(s) 8
PcsI WCGNNNNNNNCGW 2 cut(s) 104, 185
PdiI GCCGGC 1 cut(s) 347
PfeI GAWTC 2 cut(s) 287, 590
PflFI GACNNNGTC 1 cut(s) 149
PflMI CCANNNNNTGG 1 cut(s) 100
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PshAI GACNNNNGTC 1 cut(s) 167
PspN4I GGNNCC 3 cut(s) 141, 175, 262
PspPI GGNCC 1 cut(s) 139
PsuI RGATCY 1 cut(s) 74
PsyI GACNNNGTC 1 cut(s) 149
RsaI GTAC 1 cut(s) 329
RsaNI GTAC 1 cut(s) 328
RseI CAYNNNNRTG 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 8
Sau3AI GATC 4 cut(s) 74, 301, 351, 367
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 1 cut(s) 16
SduI GDGCHC 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 536
SinI GGWCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 152
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
SsiI CCGC 8 cut(s) 105, 116, 127, 194, 311, 343, 501, 545
TaaI ACNGT 4 cut(s) 139, 295, 378, 491
TaiI ACGT 3 cut(s) 101, 240, 443
TaqI TCGA 2 cut(s) 381, 536
TauI GCSGC 6 cut(s) 107, 118, 130, 197, 314, 345
TfiI GAWTC 2 cut(s) 287, 590
TseFI GTSAC 2 cut(s) 238, 372
TseI GCWGC 4 cut(s) 118, 226, 229, 314
Tsp45I GTSAC 2 cut(s) 238, 372
TspDTI ATGAA 2 cut(s) 519, 561
TspGWI ACGGA 3 cut(s) 348, 352, 360
Tth111I GACNNNGTC 1 cut(s) 149
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.