Rmu_co8020042.1_g000001

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8020042.1
OG0010479
Physical Location & Seq
Reverse (-)
141 .. 539
399 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8020042.1_g000001.1.cds

Sequence Viewer

Length: 291 bp
ggggccgtacggcagtacgagaaagaaggtcgccggccgatcttcccctccgtcgatccctcacgcttcgacctccattattcccagttcagcttagaaagtttggatagggaggagaagctgatggagttggggtcgcgcaactttttcctgtgccctaagaagtcggccgcggacggtgggagtagcggtggcggaataacgacgtcgtctggttcatgttcgaaacaggtggagaaagcttccaaaactgggttttcttggctcaagtttatggattccctgctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

96

Amino Acids

10.59

Weight (kDa)

8.66

Isoelectric Point (pI)

51.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 209
AccII CGCG 2 cut(s) 139, 173
AciI CCGC 4 cut(s) 171, 173, 189, 195
AclWI GGATC 1 cut(s) 50
AcoI YGGCCR 2 cut(s) 35, 168
AcyI GRCGYC 1 cut(s) 206
AfaI GTAC 2 cut(s) 9, 17
AfiI CCNNNNNNNGG 1 cut(s) 252
AluBI AGCT 3 cut(s) 93, 121, 242
AluI AGCT 3 cut(s) 93, 121, 242
AlwI GGATC 1 cut(s) 50
AoxI GGCC 3 cut(s) 3, 35, 168
AspLEI GCGC 1 cut(s) 141
AspS9I GGNCC 1 cut(s) 3
AsuII TTCGAA 1 cut(s) 224
BaeGI GKGCMC 1 cut(s) 158
BccI CCATC 1 cut(s) 118
BceAI ACGGC 1 cut(s) 26
BisI GCNGC 1 cut(s) 171
BlsI GCNGC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 3
BmiI GGNNCC 1 cut(s) 4
BmrI ACTGGG 2 cut(s) 79, 261
BmuI ACTGGG 2 cut(s) 79, 261
Bpu14I TTCGAA 1 cut(s) 224
BpuEI CTTGAG 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 171
BsaXI ACNNNNNCTCC 4 cut(s) 119, 149, 175, 205
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse118I RCCGGY 1 cut(s) 33
Bse1I ACTGG 2 cut(s) 85, 256
BseDI CCNNGG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 252
BseNI ACTGG 2 cut(s) 85, 256
BseRI GAGGAG 1 cut(s) 128
BseSI GKGCMC 1 cut(s) 158
BseX3I CGGCCG 2 cut(s) 35, 168
Bsh1236I CGCG 2 cut(s) 139, 173
Bsh1285I CGRYCG 2 cut(s) 38, 171
BshFI GGCC 3 cut(s) 5, 37, 170
BsiEI CGRYCG 2 cut(s) 38, 171
BsiSI CCGG 1 cut(s) 34
BsiWI CGTACG 1 cut(s) 7
BslI CCNNNNNNNGG 1 cut(s) 252
BsnI GGCC 3 cut(s) 5, 37, 170
Bsp119I TTCGAA 1 cut(s) 224
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 2 cut(s) 39, 55
BspACI CCGC 4 cut(s) 171, 173, 189, 195
BspANI GGCC 3 cut(s) 5, 37, 170
BspFNI CGCG 2 cut(s) 139, 173
BspLI GGNNCC 1 cut(s) 4
BspPI GGATC 1 cut(s) 50
BspT104I TTCGAA 1 cut(s) 224
BsrFI RCCGGY 1 cut(s) 33
BsrI ACTGG 2 cut(s) 85, 256
BssAI RCCGGY 1 cut(s) 33
BssECI CCNNGG 1 cut(s) 171
BssMI GATC 2 cut(s) 39, 55
BssNI GRCGYC 1 cut(s) 206
Bst4CI ACNGT 1 cut(s) 179
BstACI GRCGYC 1 cut(s) 206
BstBI TTCGAA 1 cut(s) 224
BstC8I GCNNGC 1 cut(s) 35
BstDEI CTNAG 2 cut(s) 94, 159
BstDSI CCRYGG 1 cut(s) 171
BstFNI CGCG 2 cut(s) 139, 173
BstHHI GCGC 1 cut(s) 141
BstKTI GATC 2 cut(s) 42, 58
BstMBI GATC 2 cut(s) 39, 55
BstMCI CGRYCG 2 cut(s) 38, 171
BstSLI GKGCMC 1 cut(s) 158
BstUI CGCG 2 cut(s) 139, 173
BstZI CGGCCG 2 cut(s) 35, 168
BsuRI GGCC 3 cut(s) 5, 37, 170
BtgI CCRYGG 1 cut(s) 171
Cac8I GCNNGC 1 cut(s) 35
CfoI GCGC 1 cut(s) 141
Cfr10I RCCGGY 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 3
Cfr42I CCGCGG 1 cut(s) 174
Csp6I GTAC 2 cut(s) 8, 16
CviAII CATG 1 cut(s) 219
CviJI RGCY 7 cut(s) 5, 37, 93, 121, 170, 242, 265
CviKI_1 RGCY 7 cut(s) 5, 37, 93, 121, 170, 242, 265
CviQI GTAC 2 cut(s) 8, 16
DdeI CTNAG 2 cut(s) 94, 159
DpnI GATC 2 cut(s) 41, 57
DpnII GATC 2 cut(s) 39, 55
EaeI YGGCCR 2 cut(s) 35, 168
EagI CGGCCG 2 cut(s) 35, 168
EciI GGCGGA 1 cut(s) 210
EclXI CGGCCG 2 cut(s) 35, 168
Eco52I CGGCCG 2 cut(s) 35, 168
FaeI CATG 1 cut(s) 222
FaiI YATR 2 cut(s) 220, 275
FatI CATG 1 cut(s) 218
Fnu4HI GCNGC 1 cut(s) 171
Fsp4HI GCNGC 1 cut(s) 171
GlaI GCGC 1 cut(s) 140
GluI GCNGC 1 cut(s) 171
HaeIII GGCC 3 cut(s) 5, 37, 170
HapII CCGG 1 cut(s) 34
HhaI GCGC 1 cut(s) 141
Hin1I GRCGYC 1 cut(s) 206
Hin1II CATG 1 cut(s) 222
Hin6I GCGC 1 cut(s) 139
HinP1I GCGC 1 cut(s) 139
HindIII AAGCTT 1 cut(s) 240
HinfI GANTC 1 cut(s) 278
HpaII CCGG 1 cut(s) 34
Hpy99I CGWCG 3 cut(s) 56, 208, 211
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 1 cut(s) 179
HpyCH4IV ACGT 1 cut(s) 206
HpyF3I CTNAG 2 cut(s) 94, 159
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 206
Hsp92II CATG 1 cut(s) 222
HspAI GCGC 1 cut(s) 139
KroI GCCGGC 1 cut(s) 33
KroNI GCCGGC 1 cut(s) 35
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 2 cut(s) 39, 55
LpnPI CCDG 6 cut(s) 47, 98, 164, 198, 215, 237
MaeII ACGT 1 cut(s) 206
MalI GATC 2 cut(s) 41, 57
MboI GATC 2 cut(s) 39, 55
MboII GAAGA 1 cut(s) 34
MhlI GDGCHC 1 cut(s) 158
MnlI CCTC 4 cut(s) 58, 70, 83, 106
MroNI GCCGGC 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 173
MspI CCGG 1 cut(s) 34
MvnI CGCG 2 cut(s) 139, 173
NaeI GCCGGC 1 cut(s) 35
NdeII GATC 2 cut(s) 39, 55
NgoMIV GCCGGC 1 cut(s) 33
NlaIII CATG 1 cut(s) 222
NlaIV GGNNCC 1 cut(s) 4
NspV TTCGAA 1 cut(s) 224
PdiI GCCGGC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 278
Pfl23II CGTACG 1 cut(s) 7
PflFI GACNNNGTC 1 cut(s) 208
PkrI GCNGC 1 cut(s) 172
PspLI CGTACG 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 4
PspPI GGNCC 1 cut(s) 3
PsyI GACNNNGTC 1 cut(s) 208
RsaI GTAC 2 cut(s) 9, 17
RsaNI GTAC 2 cut(s) 8, 16
SacII CCGCGG 1 cut(s) 174
SatI GCNGC 1 cut(s) 171
Sau3AI GATC 2 cut(s) 39, 55
Sau96I GGNCC 1 cut(s) 3
SduI GDGCHC 1 cut(s) 158
SetI ASST 7 cut(s) 31, 75, 95, 123, 209, 234, 244
Sfr303I CCGCGG 1 cut(s) 174
SfuI TTCGAA 1 cut(s) 224
SgrBI CCGCGG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SsiI CCGC 4 cut(s) 171, 173, 189, 195
TaaI ACNGT 1 cut(s) 179
TaiI ACGT 1 cut(s) 209
TaqI TCGA 3 cut(s) 54, 69, 224
TauI GCSGC 1 cut(s) 173
TfiI GAWTC 1 cut(s) 278
TspDTI ATGAA 1 cut(s) 207
TspGWI ACGGA 1 cut(s) 40
Tth111I GACNNNGTC 1 cut(s) 208
ZraI GACGTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.