Rh5BG373600

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010479
Physical Location & Seq
Forward (+)
58826549 .. 58829277
2729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG373600.1

Sequence Viewer

Length: 612 bp
ATGCCGAGTCCGAAGAGTCAGCGAAGGATGCAAGAGGAGCACAAGAACCGGAGGGGCAGATTACTCTCGGAGAGATCCTCGTCGTTTCACGGCCAGATGTTGGCCGCGACGTCGACGGCGACACCGCAGCTCCGGCGGCCGAAGACGGTCCCGGACATGGTCCTGTACAGAAACGCTGTCGGAGTCACGACGCCGCCGACTGCGTCGCCGGAGGTGCGGCCTAAGCTGACGAAGCTGCTGCTGAACGTGACGATACAAGGGAGTGTCGGTGCTGTACAGGTGGTTATGTCGCCGGAATCGACTGTCGGAGATCTTGTGGCGGCTGCAGTACGGCAGTACGAGAAAGAAGGTCGCCGGCCGATCTTCCCCTCCGTCGATCCCTCACGCTTCGACCTCCATTATTCCCAGTTCAGCTTAGAAAGTTTGGATAGGGAGGAGAAGCTGATGGAGTTGGGGTCGCGCAACTTTTTCCTGTGCCCTAAGAAGTCGGCCGCGGACGGCGGGGGTACCGGTGGCGGAATAACGACGTCGTCTGGTTCATGTTCGAAACAGGTGGAGAAAGCTTCCAAAACTGGGTTTTCTTGGCTCAAGTTTATGGATTCCCTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

22.23

Weight (kDa)

9.9

Isoelectric Point (pI)

60.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 75 - 159 4.1e-39 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 113, 530
Acc65I GGTACC 1 cut(s) 506
AccB1I GGYRCC 1 cut(s) 506
AccB7I CCANNNNNTGG 1 cut(s) 100
AccI GTMKAC 1 cut(s) 113
AccII CGCG 3 cut(s) 107, 460, 494
AclWI GGATC 2 cut(s) 69, 371
AcoI YGGCCR 5 cut(s) 91, 102, 137, 356, 489
AcyI GRCGYC 3 cut(s) 110, 191, 527
AfaI GTAC 5 cut(s) 167, 276, 330, 338, 508
AfiI CCNNNNNNNGG 3 cut(s) 100, 157, 573
AgeI ACCGGT 1 cut(s) 509
AluBI AGCT 6 cut(s) 130, 226, 235, 414, 442, 563
AluI AGCT 6 cut(s) 130, 226, 235, 414, 442, 563
Alw21I GWGCWC 1 cut(s) 42
AlwI GGATC 2 cut(s) 69, 371
AoxI GGCC 6 cut(s) 91, 102, 137, 218, 356, 489
ApeKI GCWGC 4 cut(s) 127, 235, 238, 323
AsiGI ACCGGT 1 cut(s) 509
Asp718I GGTACC 1 cut(s) 506
AspLEI GCGC 1 cut(s) 462
AspS9I GGNCC 2 cut(s) 148, 160
AsuC2I CCSGG 1 cut(s) 152
AsuII TTCGAA 1 cut(s) 545
AvaII GGWCC 2 cut(s) 148, 160
BaeGI GKGCMC 1 cut(s) 479
BanI GGYRCC 1 cut(s) 506
BbsI GAAGAC 1 cut(s) 149
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 4 cut(s) 139, 222, 225, 310
BccI CCATC 1 cut(s) 439
BceAI ACGGC 4 cut(s) 106, 132, 347, 514
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BcnI CCSGG 1 cut(s) 152
BfmI CTRYAG 1 cut(s) 324
BglII AGATCT 1 cut(s) 310
Bme1390I CCNGG 1 cut(s) 152
Bme18I GGWCC 2 cut(s) 148, 160
BmgT120I GGNCC 2 cut(s) 148, 160
BmiI GGNNCC 2 cut(s) 150, 508
BmrFI CCNGG 1 cut(s) 152
BmrI ACTGGG 2 cut(s) 400, 582
BmsI GCATC 1 cut(s) 18
BmuI ACTGGG 2 cut(s) 400, 582
BpiI GAAGAC 1 cut(s) 149
BplI GAGNNNNNCTC 2 cut(s) 62, 94
Bpu10I CCTNAGC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 545
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 1 cut(s) 152
BsaHI GRCGYC 3 cut(s) 110, 191, 527
BsaJI CCNNGG 1 cut(s) 492
BsaWI WCCGGW 2 cut(s) 48, 509
BsaXI ACNNNNNCTCC 8 cut(s) 114, 144, 253, 283, 300, 330, 440, 470
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 157, 573
Bse118I RCCGGY 2 cut(s) 354, 509
Bse1I ACTGG 2 cut(s) 406, 577
BseDI CCNNGG 1 cut(s) 492
BseGI GGATG 1 cut(s) 33
BseLI CCNNNNNNNGG 3 cut(s) 100, 157, 573
BseNI ACTGG 2 cut(s) 406, 577
BseRI GAGGAG 2 cut(s) 50, 449
BseSI GKGCMC 1 cut(s) 479
BseX3I CGGCCG 3 cut(s) 137, 356, 489
BseXI GCAGC 4 cut(s) 139, 222, 225, 310
Bsh1236I CGCG 3 cut(s) 107, 460, 494
Bsh1285I CGRYCG 3 cut(s) 140, 359, 492
BshFI GGCC 6 cut(s) 93, 104, 139, 220, 358, 491
BshNI GGYRCC 1 cut(s) 506
BshTI ACCGGT 1 cut(s) 509
BsiEI CGRYCG 3 cut(s) 140, 359, 492
BsiHKAI GWGCWC 1 cut(s) 42
BsiSI CCGG 7 cut(s) 49, 133, 152, 209, 293, 355, 510
BslFI GGGAC 1 cut(s) 134
BslI CCNNNNNNNGG 3 cut(s) 100, 157, 573
BsmFI GGGAC 1 cut(s) 134
BsnI GGCC 6 cut(s) 93, 104, 139, 220, 358, 491
Bsp119I TTCGAA 1 cut(s) 545
Bsp1286I GDGCHC 2 cut(s) 42, 479
Bsp1407I TGTACA 2 cut(s) 165, 274
Bsp143I GATC 4 cut(s) 74, 310, 360, 376
BspANI GGCC 6 cut(s) 93, 104, 139, 220, 358, 491
BspFNI CGCG 3 cut(s) 107, 460, 494
BspLI GGNNCC 2 cut(s) 150, 508
BspMAI CTGCAG 1 cut(s) 328
BspPI GGATC 2 cut(s) 69, 371
BspT104I TTCGAA 1 cut(s) 545
BspT107I GGYRCC 1 cut(s) 506
BsrFI RCCGGY 2 cut(s) 354, 509
BsrGI TGTACA 2 cut(s) 165, 274
BsrI ACTGG 2 cut(s) 406, 577
BssAI RCCGGY 2 cut(s) 354, 509
BssECI CCNNGG 1 cut(s) 492
BssMI GATC 4 cut(s) 74, 310, 360, 376
BssNI GRCGYC 3 cut(s) 110, 191, 527
Bst4CI ACNGT 2 cut(s) 148, 304
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 3 cut(s) 110, 191, 527
BstAUI TGTACA 2 cut(s) 165, 274
BstBI TTCGAA 1 cut(s) 545
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 3 cut(s) 222, 415, 480
BstDSI CCRYGG 1 cut(s) 492
BstF5I GGATG 1 cut(s) 33
BstFNI CGCG 3 cut(s) 107, 460, 494
BstHHI GCGC 1 cut(s) 462
BstKTI GATC 4 cut(s) 77, 313, 363, 379
BstMBI GATC 4 cut(s) 74, 310, 360, 376
BstMCI CGRYCG 3 cut(s) 140, 359, 492
BstMWI GCNNNNNNNGC 7 cut(s) 28, 37, 133, 136, 214, 223, 232
BstSCI CCNGG 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 324
BstSLI GKGCMC 1 cut(s) 479
BstUI CGCG 3 cut(s) 107, 460, 494
BstV1I GCAGC 4 cut(s) 139, 222, 225, 310
BstV2I GAAGAC 1 cut(s) 149
BstX2I RGATCY 2 cut(s) 74, 310
BstYI RGATCY 2 cut(s) 74, 310
BstZI CGGCCG 3 cut(s) 137, 356, 489
BsuRI GGCC 6 cut(s) 93, 104, 139, 220, 358, 491
BtgI CCRYGG 1 cut(s) 492
BtsCI GGATG 1 cut(s) 33
Cac8I GCNNGC 1 cut(s) 356
CfoI GCGC 1 cut(s) 462
Cfr10I RCCGGY 2 cut(s) 354, 509
Cfr13I GGNCC 2 cut(s) 148, 160
Cfr42I CCGCGG 1 cut(s) 495
CseI GACGC 2 cut(s) 192, 199
Csp6I GTAC 5 cut(s) 166, 275, 329, 337, 507
CspAI ACCGGT 1 cut(s) 509
CviAII CATG 2 cut(s) 157, 540
CviQI GTAC 5 cut(s) 166, 275, 329, 337, 507
DdeI CTNAG 3 cut(s) 222, 415, 480
DpnI GATC 4 cut(s) 76, 312, 362, 378
DpnII GATC 4 cut(s) 74, 310, 360, 376
EaeI YGGCCR 5 cut(s) 91, 102, 137, 356, 489
EagI CGGCCG 3 cut(s) 137, 356, 489
Eam1104I CTCTTC 1 cut(s) 8
EarI CTCTTC 1 cut(s) 8
EciI GGCGGA 1 cut(s) 531
EclXI CGGCCG 3 cut(s) 137, 356, 489
Eco47I GGWCC 2 cut(s) 148, 160
Eco52I CGGCCG 3 cut(s) 137, 356, 489
FaeI CATG 2 cut(s) 160, 543
FaiI YATR 4 cut(s) 158, 287, 541, 596
FaqI GGGAC 1 cut(s) 134
FatI CATG 2 cut(s) 156, 539
FauI CCCGC 1 cut(s) 494
FblI GTMKAC 1 cut(s) 113
FokI GGATG 1 cut(s) 40
GlaI GCGC 1 cut(s) 461
HaeIII GGCC 6 cut(s) 93, 104, 139, 220, 358, 491
HapII CCGG 7 cut(s) 49, 133, 152, 209, 293, 355, 510
HgaI GACGC 2 cut(s) 192, 199
HhaI GCGC 1 cut(s) 462
Hin1I GRCGYC 3 cut(s) 110, 191, 527
Hin1II CATG 2 cut(s) 160, 543
Hin6I GCGC 1 cut(s) 460
HinP1I GCGC 1 cut(s) 460
HincII GTYRAC 1 cut(s) 114
HindII GTYRAC 1 cut(s) 114
HindIII AAGCTT 1 cut(s) 561
HinfI GANTC 5 cut(s) 7, 16, 183, 296, 599
HpaII CCGG 7 cut(s) 49, 133, 152, 209, 293, 355, 510
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 4 cut(s) 12, 70, 182, 308
Hpy188III TCNNGA 1 cut(s) 187
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 9 cut(s) 85, 112, 115, 118, 193, 208, 377, 529, 532
HpyAV CCTTC 2 cut(s) 18, 341
HpyCH4III ACNGT 2 cut(s) 148, 304
HpyCH4IV ACGT 3 cut(s) 110, 246, 527
HpyCH4V TGCA 2 cut(s) 31, 326
HpyF10VI GCNNNNNNNGC 7 cut(s) 28, 37, 133, 136, 214, 223, 232
HpyF3I CTNAG 3 cut(s) 222, 415, 480
HpySE526I ACGT 3 cut(s) 110, 246, 527
Hsp92I GRCGYC 3 cut(s) 110, 191, 527
Hsp92II CATG 2 cut(s) 160, 543
HspAI GCGC 1 cut(s) 460
KpnI GGTACC 1 cut(s) 510
KroI GCCGGC 1 cut(s) 354
KroNI GCCGGC 1 cut(s) 356
KspI CCGCGG 1 cut(s) 495
Kzo9I GATC 4 cut(s) 74, 310, 360, 376
LmnI GCTCC 2 cut(s) 37, 135
Lsp1109I GCAGC 4 cut(s) 139, 222, 225, 310
LweI GCATC 1 cut(s) 18
MaeII ACGT 3 cut(s) 110, 246, 527
MaeIII GTNAC 2 cut(s) 184, 247
MalI GATC 4 cut(s) 76, 312, 362, 378
MboI GATC 4 cut(s) 74, 310, 360, 376
MboII GAAGA 3 cut(s) 25, 154, 355
MflI RGATCY 2 cut(s) 74, 310
MhlI GDGCHC 2 cut(s) 42, 479
MlyI GAGTC 3 cut(s) 16, 25, 192
MmeI TCCRAC 2 cut(s) 160, 286
MnlI CCTC 8 cut(s) 28, 45, 88, 205, 379, 391, 404, 427
MroNI GCCGGC 1 cut(s) 354
MspA1I CMGCKG 1 cut(s) 494
MspI CCGG 7 cut(s) 49, 133, 152, 209, 293, 355, 510
MspR9I CCNGG 1 cut(s) 152
MvnI CGCG 3 cut(s) 107, 460, 494
MwoI GCNNNNNNNGC 7 cut(s) 28, 37, 133, 136, 214, 223, 232
NaeI GCCGGC 1 cut(s) 356
NciI CCSGG 1 cut(s) 152
NdeII GATC 4 cut(s) 74, 310, 360, 376
NgoMIV GCCGGC 1 cut(s) 354
NlaIII CATG 2 cut(s) 160, 543
NlaIV GGNNCC 2 cut(s) 150, 508
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 184, 247
NspV TTCGAA 1 cut(s) 545
PcsI WCGNNNNNNNCGW 3 cut(s) 116, 194, 296
PdiI GCCGGC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 296, 599
PflFI GACNNNGTC 3 cut(s) 158, 202, 529
PflMI CCANNNNNTGG 1 cut(s) 100
PfoI TCCNGGA 1 cut(s) 150
PinAI ACCGGT 1 cut(s) 509
PleI GAGTC 3 cut(s) 15, 24, 191
PpsI GAGTC 3 cut(s) 15, 24, 191
PspN4I GGNNCC 2 cut(s) 150, 508
PspPI GGNCC 2 cut(s) 148, 160
PstI CTGCAG 1 cut(s) 328
PsuI RGATCY 2 cut(s) 74, 310
PsyI GACNNNGTC 3 cut(s) 158, 202, 529
RsaI GTAC 5 cut(s) 167, 276, 330, 338, 508
RsaNI GTAC 5 cut(s) 166, 275, 329, 337, 507
SacII CCGCGG 1 cut(s) 495
SalI GTCGAC 1 cut(s) 112
Sau3AI GATC 4 cut(s) 74, 310, 360, 376
Sau96I GGNCC 2 cut(s) 148, 160
SchI GAGTC 3 cut(s) 16, 25, 192
ScrFI CCNGG 1 cut(s) 152
SduI GDGCHC 2 cut(s) 42, 479
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 1 cut(s) 324
Sfr303I CCGCGG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 545
SgrBI CCGCGG 1 cut(s) 495
SgrDI CGTCGACG 1 cut(s) 112
SinI GGWCC 2 cut(s) 148, 160
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
StyD4I CCNGG 1 cut(s) 150
TaaI ACNGT 2 cut(s) 148, 304
TaiI ACGT 3 cut(s) 113, 249, 530
TaqI TCGA 5 cut(s) 113, 299, 375, 390, 545
TatI WGTACW 2 cut(s) 165, 274
TauI GCSGC 6 cut(s) 107, 139, 196, 220, 323, 494
TfiI GAWTC 2 cut(s) 296, 599
TseFI GTSAC 2 cut(s) 184, 247
TseI GCWGC 4 cut(s) 127, 235, 238, 323
Tsp45I GTSAC 2 cut(s) 184, 247
TspDTI ATGAA 1 cut(s) 528
TspGWI ACGGA 1 cut(s) 361
Tth111I GACNNNGTC 3 cut(s) 158, 202, 529
Van91I CCANNNNNTGG 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 148, 160
XmiI GTMKAC 1 cut(s) 113
ZraI GACGTC 2 cut(s) 111, 528
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.