MD11G1156700.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010479
Physical Location & Seq
Reverse (-)
15082886 .. 15083485
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1156700.v1.1.491

Sequence Viewer

Length: 354 bp
ATGGCGACGCAGCAGCTTCGGAGGCCGAAAACGGTGCCGGACATGGCGTCGTTCAGGAACGCGGTGGGAACGACAGCGTCGTTGATGGAATTACGGCCGAAGCTGACGAAGCTGCTCCTCAACGTTACGATCCAGGGGAGCGTTGGGGCCATACAGGTCGTGATGTCGCCGGAACTGACGGAGAAGCTGATGGAGTTGGGATCCAGGAACTTCTTTCTGTGCCCTAACGCAAAAGCCGTGGGCGGTGGAGGTAGCGGTGGAGCGACGGCGTCGTCCGCGTCATGTTCAAAAGAGGCGGAGAAAGTTTCAAGGAATGGGTTTGCGTGGCTGAAGTTCATGAATTTCATGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

118

Amino Acids

12.48

Weight (kDa)

10.02

Isoelectric Point (pI)

40.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 34 - 58 8.5e-06 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 271
AccB1I GGYRCC 1 cut(s) 34
AccII CGCG 2 cut(s) 62, 278
AciI CCGC 5 cut(s) 62, 243, 255, 276, 296
AclI AACGTT 1 cut(s) 123
AclWI GGATC 3 cut(s) 124, 195, 208
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 1 cut(s) 340
AcuI CTGAAG 1 cut(s) 350
AcyI GRCGYC 2 cut(s) 47, 269
AgsI TTSAA 2 cut(s) 288, 309
AhdI GACNNNNNGTC 1 cut(s) 46
AjnI CCWGG 2 cut(s) 132, 203
AluBI AGCT 4 cut(s) 16, 103, 112, 187
AluI AGCT 4 cut(s) 16, 103, 112, 187
AlwI GGATC 3 cut(s) 124, 195, 208
AoxI GGCC 3 cut(s) 23, 95, 147
ApeKI GCWGC 3 cut(s) 10, 13, 112
ApoI RAATTY 1 cut(s) 340
AspS9I GGNCC 1 cut(s) 147
BaeGI GKGCMC 1 cut(s) 224
BamHI GGATCC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 34
BbvI GCAGC 3 cut(s) 22, 25, 99
BccI CCATC 2 cut(s) 79, 184
BceAI ACGGC 3 cut(s) 110, 221, 282
BcgI CGANNNNNNTGC 3 cut(s) 16, 33, 50
BciT130I CCWGG 2 cut(s) 134, 205
BisI GCNGC 3 cut(s) 11, 14, 113
BlsI GCNGC 3 cut(s) 12, 15, 114
Bme1390I CCNGG 2 cut(s) 134, 205
BmeRI GACNNNNNGTC 1 cut(s) 46
BmgT120I GGNCC 1 cut(s) 147
BmiI GGNNCC 3 cut(s) 36, 148, 202
BmrFI CCNGG 2 cut(s) 134, 205
BsaHI GRCGYC 2 cut(s) 47, 269
BsaJI CCNNGG 2 cut(s) 133, 237
BseBI CCWGG 2 cut(s) 134, 205
BseDI CCNNGG 2 cut(s) 133, 237
BseRI GAGGAG 1 cut(s) 107
BseSI GKGCMC 1 cut(s) 224
BseX3I CGGCCG 1 cut(s) 95
BseXI GCAGC 3 cut(s) 22, 25, 99
Bsh1236I CGCG 2 cut(s) 62, 278
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 3 cut(s) 25, 97, 149
BshNI GGYRCC 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 98
BsiSI CCGG 2 cut(s) 38, 170
BsnI GGCC 3 cut(s) 25, 97, 149
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 2 cut(s) 129, 200
BspACI CCGC 5 cut(s) 62, 243, 255, 276, 296
BspANI GGCC 3 cut(s) 25, 97, 149
BspFNI CGCG 2 cut(s) 62, 278
BspHI TCATGA 1 cut(s) 336
BspLI GGNNCC 3 cut(s) 36, 148, 202
BspPI GGATC 3 cut(s) 124, 195, 208
BspT107I GGYRCC 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 133, 237
BssMI GATC 2 cut(s) 129, 200
BssNI GRCGYC 2 cut(s) 47, 269
Bst2UI CCWGG 2 cut(s) 134, 205
Bst4CI ACNGT 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 47, 269
BstDSI CCRYGG 1 cut(s) 237
BstFNI CGCG 2 cut(s) 62, 278
BstKTI GATC 2 cut(s) 132, 203
BstMBI GATC 2 cut(s) 129, 200
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 3 cut(s) 22, 109, 275
BstNI CCWGG 2 cut(s) 134, 205
BstSCI CCNGG 2 cut(s) 132, 203
BstSLI GKGCMC 1 cut(s) 224
BstUI CGCG 2 cut(s) 62, 278
BstV1I GCAGC 3 cut(s) 22, 25, 99
BstX2I RGATCY 1 cut(s) 200
BstYI RGATCY 1 cut(s) 200
BstZI CGGCCG 1 cut(s) 95
BsuRI GGCC 3 cut(s) 25, 97, 149
BtgI CCRYGG 1 cut(s) 237
CciI TCATGA 1 cut(s) 336
Cfr13I GGNCC 1 cut(s) 147
CseI GACGC 5 cut(s) 16, 36, 66, 258, 267
CspCI CAANNNNNGTGG 2 cut(s) 219, 254
CviAII CATG 4 cut(s) 43, 282, 337, 346
CviJI RGCY 9 cut(s) 16, 25, 97, 103, 112, 149, 187, 236, 328
CviKI_1 RGCY 9 cut(s) 16, 25, 97, 103, 112, 149, 187, 236, 328
DpnI GATC 2 cut(s) 131, 202
DpnII GATC 2 cut(s) 129, 200
DrdI GACNNNNNNGTC 1 cut(s) 271
DriI GACNNNNNGTC 1 cut(s) 46
DseDI GACNNNNNNGTC 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 95
EagI CGGCCG 1 cut(s) 95
Eam1105I GACNNNNNGTC 1 cut(s) 46
EciI GGCGGA 1 cut(s) 311
EclXI CGGCCG 1 cut(s) 95
Eco52I CGGCCG 1 cut(s) 95
Eco57I CTGAAG 1 cut(s) 350
EcoRII CCWGG 2 cut(s) 132, 203
FaeI CATG 4 cut(s) 46, 285, 340, 349
FaiI YATR 5 cut(s) 44, 152, 283, 338, 347
FatI CATG 4 cut(s) 42, 281, 336, 345
Fnu4HI GCNGC 3 cut(s) 11, 14, 113
Fsp4HI GCNGC 3 cut(s) 11, 14, 113
GluI GCNGC 3 cut(s) 11, 14, 113
HaeIII GGCC 3 cut(s) 25, 97, 149
HapII CCGG 2 cut(s) 38, 170
HgaI GACGC 5 cut(s) 16, 36, 66, 258, 267
Hin1I GRCGYC 2 cut(s) 47, 269
Hin1II CATG 4 cut(s) 46, 285, 340, 349
HpaII CCGG 2 cut(s) 38, 170
Hpy188I TCNGA 1 cut(s) 21
Hpy188III TCNNGA 3 cut(s) 55, 160, 337
Hpy99I CGWCG 5 cut(s) 10, 52, 82, 268, 274
HpyCH4III ACNGT 1 cut(s) 34
HpyCH4IV ACGT 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 3 cut(s) 22, 109, 275
HpySE526I ACGT 1 cut(s) 123
Hsp92I GRCGYC 2 cut(s) 47, 269
Hsp92II CATG 4 cut(s) 46, 285, 340, 349
Kzo9I GATC 2 cut(s) 129, 200
LmnI GCTCC 3 cut(s) 120, 138, 260
LpnPI CCDG 8 cut(s) 40, 51, 119, 140, 146, 183, 190, 217
Lsp1109I GCAGC 3 cut(s) 22, 25, 99
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 124
MalI GATC 2 cut(s) 131, 202
MboI GATC 2 cut(s) 129, 200
MflI RGATCY 1 cut(s) 200
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 2 cut(s) 89, 340
MnlI CCTC 4 cut(s) 15, 128, 242, 286
MspI CCGG 2 cut(s) 38, 170
MspR9I CCNGG 2 cut(s) 134, 205
MvaI CCWGG 2 cut(s) 134, 205
MvnI CGCG 2 cut(s) 62, 278
MwoI GCNNNNNNNGC 3 cut(s) 22, 109, 275
NdeII GATC 2 cut(s) 129, 200
NlaIII CATG 4 cut(s) 46, 285, 340, 349
NlaIV GGNNCC 3 cut(s) 36, 148, 202
PagI TCATGA 1 cut(s) 336
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 234
PflFI GACNNNGTC 2 cut(s) 76, 268
PfoI TCCNGGA 1 cut(s) 203
PkrI GCNGC 3 cut(s) 12, 15, 114
Psp1406I AACGTT 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 132, 203
PspGI CCWGG 2 cut(s) 132, 203
PspN4I GGNNCC 3 cut(s) 36, 148, 202
PspPI GGNCC 1 cut(s) 147
PsuI RGATCY 1 cut(s) 200
PsyI GACNNNGTC 2 cut(s) 76, 268
SatI GCNGC 3 cut(s) 11, 14, 113
Sau3AI GATC 2 cut(s) 129, 200
Sau96I GGNCC 1 cut(s) 147
ScrFI CCNGG 2 cut(s) 134, 205
SduI GDGCHC 1 cut(s) 224
SetI ASST 7 cut(s) 18, 105, 114, 126, 159, 189, 253
Sse9I AATT 2 cut(s) 89, 340
SsiI CCGC 5 cut(s) 62, 243, 255, 276, 296
StyD4I CCNGG 2 cut(s) 132, 203
TaaI ACNGT 1 cut(s) 34
TaiI ACGT 1 cut(s) 126
TasI AATT 2 cut(s) 89, 340
TseI GCWGC 3 cut(s) 10, 13, 112
TspDTI ATGAA 3 cut(s) 325, 334, 353
TspGWI ACGGA 1 cut(s) 194
Tth111I GACNNNGTC 2 cut(s) 76, 268
XapI RAATTY 1 cut(s) 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 140
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.