Prupe.6G118800_v2.0.a1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0010479
Physical Location & Seq
Reverse (-)
8751401 .. 8753824
2424 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G118800.2

Sequence Viewer

Length: 582 bp
ATGCCGAGTCCGAAGAGTACTCATCGGAGGGTCCAGGAGGAGAAGAATCGCAGGGGGAGATCACTCAACGAGAAATCCTCATCGTTTCATGGCCAGCTTTCGGATATGGCGACGCCGCAGCTTCGGAGACCAAAAACGGTGCCAGACATGGCGTCGTTCAGGAACACGATCGGAATGACACCGGCCACGGAGTTACTGCCGAAACTGACGAAGCTGCTCCTCAACGTGACGATTCAGGGGAGCGTCGGCGCAATACATGTCGTAATGCCGCCGGAATCCGCGGTGAAAGATCTGGTGGCGGCTTCCATACGACAATACGGAAAGGAAGGTCGCCGTCCGATCTTGACCTCCGTCGACCCCTCGTTGTTTGACCTCCATTACTCTCAGTTCAGCTTAGAAAGTTTGGATAGAGAGGAGAAGCTGATGGCGTTAGGATCGAGGAACTTTTTTCTGTGCCGAAGCTCAAACTCACTGGATGCTGGCGGCAGTGGAACGACGTCGTCGGCTTCATGTTCAAAACAAGCAGAGAAAGTTTCAAGGACTGGATTTTCATGGCTCAAGTTTATGGATTTCTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

21.35

Weight (kDa)

9.89

Isoelectric Point (pI)

60.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 500
AccB1I GGYRCC 1 cut(s) 139
AccI GTMKAC 1 cut(s) 354
AccII CGCG 1 cut(s) 281
AciI CCGC 6 cut(s) 116, 269, 279, 281, 299, 483
AclWI GGATC 1 cut(s) 442
AcoI YGGCCR 2 cut(s) 91, 183
AcyI GRCGYC 3 cut(s) 113, 152, 497
AfaI GTAC 1 cut(s) 19
AfiI CCNNNNNNNGG 1 cut(s) 100
AflIII ACRYGT 1 cut(s) 256
AgsI TTSAA 2 cut(s) 516, 537
AhdI GACNNNNNGTC 1 cut(s) 151
AjnI CCWGG 1 cut(s) 33
AluBI AGCT 6 cut(s) 97, 121, 214, 393, 421, 462
AluI AGCT 6 cut(s) 97, 121, 214, 393, 421, 462
Alw26I GTCTC 1 cut(s) 121
AlwI GGATC 1 cut(s) 442
AoxI GGCC 2 cut(s) 91, 183
ApeKI GCWGC 2 cut(s) 118, 214
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 31
BalI TGGCCA 1 cut(s) 93
BanI GGYRCC 1 cut(s) 139
BbvI GCAGC 2 cut(s) 130, 201
BccI CCATC 1 cut(s) 418
BceAI ACGGC 1 cut(s) 318
BciT130I CCWGG 1 cut(s) 35
BcoDI GTCTC 1 cut(s) 121
BglII AGATCT 1 cut(s) 289
BisI GCNGC 6 cut(s) 116, 119, 215, 269, 300, 484
BlsI GCNGC 6 cut(s) 117, 120, 216, 270, 301, 485
BmcAI AGTACT 1 cut(s) 19
Bme1390I CCNGG 1 cut(s) 35
Bme18I GGWCC 1 cut(s) 31
BmeRI GACNNNNNGTC 1 cut(s) 151
BmgT120I GGNCC 1 cut(s) 31
BmiI GGNNCC 2 cut(s) 32, 141
BmrFI CCNGG 1 cut(s) 35
BmsI GCATC 1 cut(s) 466
BoxI GACNNNNGTC 1 cut(s) 350
BplI GAGNNNNNCTC 2 cut(s) 62, 94
BpuEI CTTGAG 1 cut(s) 542
BsaHI GRCGYC 3 cut(s) 113, 152, 497
BsaI GGTCTC 1 cut(s) 121
BsaJI CCNNGG 2 cut(s) 186, 279
Bsc4I CCNNNNNNNGG 1 cut(s) 100
Bse118I RCCGGY 1 cut(s) 181
Bse1I ACTGG 2 cut(s) 477, 547
BseBI CCWGG 1 cut(s) 35
BseDI CCNNGG 2 cut(s) 186, 279
BseGI GGATG 1 cut(s) 481
BseLI CCNNNNNNNGG 1 cut(s) 100
BseMII CTCAG 1 cut(s) 398
BseNI ACTGG 2 cut(s) 477, 547
BseRI GAGGAG 3 cut(s) 53, 209, 428
BseXI GCAGC 2 cut(s) 130, 201
Bsh1236I CGCG 1 cut(s) 281
Bsh1285I CGRYCG 1 cut(s) 171
BshFI GGCC 2 cut(s) 93, 185
BshNI GGYRCC 1 cut(s) 139
BsiEI CGRYCG 1 cut(s) 171
BsiSI CCGG 2 cut(s) 182, 272
BslI CCNNNNNNNGG 1 cut(s) 100
BsmAI GTCTC 1 cut(s) 121
BsnI GGCC 2 cut(s) 93, 185
Bso31I GGTCTC 1 cut(s) 121
Bsp143I GATC 5 cut(s) 59, 168, 289, 339, 434
BspACI CCGC 6 cut(s) 116, 269, 279, 281, 299, 483
BspANI GGCC 2 cut(s) 93, 185
BspCNI CTCAG 1 cut(s) 397
BspFNI CGCG 1 cut(s) 281
BspLI GGNNCC 2 cut(s) 32, 141
BspPI GGATC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 139
BspTNI GGTCTC 1 cut(s) 121
BsrFI RCCGGY 1 cut(s) 181
BsrI ACTGG 2 cut(s) 477, 547
BssAI RCCGGY 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 186, 279
BssMI GATC 5 cut(s) 59, 168, 289, 339, 434
BssNI GRCGYC 3 cut(s) 113, 152, 497
Bst2UI CCWGG 1 cut(s) 35
Bst4CI ACNGT 1 cut(s) 139
Bst6I CTCTTC 1 cut(s) 8
BstACI GRCGYC 3 cut(s) 113, 152, 497
BstC8I GCNNGC 2 cut(s) 95, 481
BstDEI CTNAG 2 cut(s) 384, 394
BstDSI CCRYGG 2 cut(s) 186, 279
BstF5I GGATG 1 cut(s) 481
BstFNI CGCG 1 cut(s) 281
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 5 cut(s) 62, 171, 292, 342, 437
BstMAI GTCTC 1 cut(s) 121
BstMBI GATC 5 cut(s) 59, 168, 289, 339, 434
BstMCI CGRYCG 1 cut(s) 171
BstNI CCWGG 1 cut(s) 35
BstNSI RCATGY 1 cut(s) 260
BstPAI GACNNNNGTC 1 cut(s) 350
BstSCI CCNGG 1 cut(s) 33
BstUI CGCG 1 cut(s) 281
BstV1I GCAGC 2 cut(s) 130, 201
BstX2I RGATCY 1 cut(s) 289
BstYI RGATCY 1 cut(s) 289
BsuRI GGCC 2 cut(s) 93, 185
BtgI CCRYGG 2 cut(s) 186, 279
BtsCI GGATG 1 cut(s) 481
BtsI GCAGTG 1 cut(s) 493
BtsIMutI CAGTG 2 cut(s) 470, 493
Cac8I GCNNGC 2 cut(s) 95, 481
CfoI GCGC 1 cut(s) 251
Cfr10I RCCGGY 1 cut(s) 181
Cfr13I GGNCC 1 cut(s) 31
Cfr42I CCGCGG 1 cut(s) 282
CseI GACGC 3 cut(s) 121, 141, 232
Csp6I GTAC 1 cut(s) 18
CviAII CATG 5 cut(s) 89, 148, 257, 510, 552
CviQI GTAC 1 cut(s) 18
DdeI CTNAG 2 cut(s) 384, 394
DpnI GATC 5 cut(s) 61, 170, 291, 341, 436
DpnII GATC 5 cut(s) 59, 168, 289, 339, 434
DriI GACNNNNNGTC 1 cut(s) 151
EaeI YGGCCR 2 cut(s) 91, 183
Eam1104I CTCTTC 1 cut(s) 8
Eam1105I GACNNNNNGTC 1 cut(s) 151
EarI CTCTTC 1 cut(s) 8
Eco31I GGTCTC 1 cut(s) 121
Eco47I GGWCC 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 33
FaeI CATG 5 cut(s) 92, 151, 260, 513, 555
FaiI YATR 8 cut(s) 90, 107, 149, 258, 308, 511, 553, 566
FatI CATG 5 cut(s) 88, 147, 256, 509, 551
FblI GTMKAC 1 cut(s) 354
Fnu4HI GCNGC 6 cut(s) 116, 119, 215, 269, 300, 484
FokI GGATG 1 cut(s) 488
Fsp4HI GCNGC 6 cut(s) 116, 119, 215, 269, 300, 484
GlaI GCGC 1 cut(s) 250
GluI GCNGC 6 cut(s) 116, 119, 215, 269, 300, 484
HaeIII GGCC 2 cut(s) 93, 185
HapII CCGG 2 cut(s) 182, 272
HgaI GACGC 3 cut(s) 121, 141, 232
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 3 cut(s) 113, 152, 497
Hin1II CATG 5 cut(s) 92, 151, 260, 513, 555
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 355
HindII GTYRAC 1 cut(s) 355
HinfI GANTC 4 cut(s) 7, 46, 232, 275
HpaII CCGG 2 cut(s) 182, 272
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 1 cut(s) 355
Hpy188I TCNGA 6 cut(s) 12, 27, 103, 126, 173, 339
Hpy188III TCNNGA 2 cut(s) 160, 343
Hpy8I GTNNAC 1 cut(s) 355
Hpy99I CGWCG 7 cut(s) 115, 157, 248, 356, 499, 502, 505
HpyAV CCTTC 1 cut(s) 320
HpyCH4III ACNGT 1 cut(s) 139
HpyCH4IV ACGT 2 cut(s) 225, 497
HpyF3I CTNAG 2 cut(s) 384, 394
HpySE526I ACGT 2 cut(s) 225, 497
Hsp92I GRCGYC 3 cut(s) 113, 152, 497
Hsp92II CATG 5 cut(s) 92, 151, 260, 513, 555
HspAI GCGC 1 cut(s) 249
KspI CCGCGG 1 cut(s) 282
Kzo9I GATC 5 cut(s) 59, 168, 289, 339, 434
LmnI GCTCC 2 cut(s) 222, 240
Lsp1109I GCAGC 2 cut(s) 130, 201
LweI GCATC 1 cut(s) 466
MaeII ACGT 2 cut(s) 225, 497
MaeIII GTNAC 2 cut(s) 192, 226
MalI GATC 5 cut(s) 61, 170, 291, 341, 436
MboI GATC 5 cut(s) 59, 168, 289, 339, 434
MboII GAAGA 2 cut(s) 25, 55
MflI RGATCY 1 cut(s) 289
MlsI TGGCCA 1 cut(s) 93
MluNI TGGCCA 1 cut(s) 93
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 9 cut(s) 21, 31, 88, 230, 358, 370, 383, 406, 432
Mox20I TGGCCA 1 cut(s) 93
MscI TGGCCA 1 cut(s) 93
Msp20I TGGCCA 1 cut(s) 93
MspA1I CMGCKG 1 cut(s) 281
MspI CCGG 2 cut(s) 182, 272
MspR9I CCNGG 1 cut(s) 35
MvaI CCWGG 1 cut(s) 35
MvnI CGCG 1 cut(s) 281
NdeII GATC 5 cut(s) 59, 168, 289, 339, 434
NlaIII CATG 5 cut(s) 92, 151, 260, 513, 555
NlaIV GGNNCC 2 cut(s) 32, 141
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 1 cut(s) 226
NspI RCATGY 1 cut(s) 260
PciI ACATGT 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PfeI GAWTC 3 cut(s) 46, 232, 275
PflFI GACNNNGTC 1 cut(s) 499
PfoI TCCNGGA 1 cut(s) 33
PkrI GCNGC 6 cut(s) 117, 120, 216, 270, 301, 485
Ple19I CGATCG 1 cut(s) 171
PleI GAGTC 1 cut(s) 15
PpsI GAGTC 1 cut(s) 15
PscI ACATGT 1 cut(s) 256
PshAI GACNNNNGTC 1 cut(s) 350
Psp6I CCWGG 1 cut(s) 33
PspGI CCWGG 1 cut(s) 33
PspN4I GGNNCC 2 cut(s) 32, 141
PspPI GGNCC 1 cut(s) 31
PsuI RGATCY 1 cut(s) 289
PsyI GACNNNGTC 1 cut(s) 499
PvuI CGATCG 1 cut(s) 171
RsaI GTAC 1 cut(s) 19
RsaNI GTAC 1 cut(s) 18
SacII CCGCGG 1 cut(s) 282
SalI GTCGAC 1 cut(s) 353
SatI GCNGC 6 cut(s) 116, 119, 215, 269, 300, 484
Sau3AI GATC 5 cut(s) 59, 168, 289, 339, 434
Sau96I GGNCC 1 cut(s) 31
ScaI AGTACT 1 cut(s) 19
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 35
SfaNI GCATC 1 cut(s) 466
Sfr303I CCGCGG 1 cut(s) 282
SgrBI CCGCGG 1 cut(s) 282
SinI GGWCC 1 cut(s) 31
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SsiI CCGC 6 cut(s) 116, 269, 279, 281, 299, 483
StyD4I CCNGG 1 cut(s) 33
TaaI ACNGT 1 cut(s) 139
TaiI ACGT 2 cut(s) 228, 500
TaqI TCGA 2 cut(s) 354, 437
TatI WGTACW 1 cut(s) 17
TauI GCSGC 4 cut(s) 118, 271, 302, 486
TfiI GAWTC 3 cut(s) 46, 232, 275
TscAI CASTG 2 cut(s) 477, 493
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 118, 214
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 3 cut(s) 77, 498, 540
TspGWI ACGGA 3 cut(s) 203, 333, 340
TspRI CASTG 2 cut(s) 477, 493
Tth111I GACNNNGTC 1 cut(s) 499
VpaK11BI GGWCC 1 cut(s) 31
XceI RCATGY 1 cut(s) 260
XmiI GTMKAC 1 cut(s) 354
ZraI GACGTC 1 cut(s) 498
ZrmI AGTACT 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.