MD03G1134500.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010479
Physical Location & Seq
Reverse (-)
13411328 .. 13417428
6101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1134500.v1.1.491

Sequence Viewer

Length: 594 bp
ATGCCGAGCCCGAAGATTTCTAGCCGGAGGGGGCAGCCGGAAGAGAAGAACCGCAGGCCGAGACCGCTCTCTGTTCGATCGTCATCGTTCCACGGCCAGTTCACGGATGCGGCGGCGCCGCAGCTTCGGAGGCCCAAAACGGTGCCGGATATGGCGTCGTTCAGGAACGCAGTCGGAACAACGGTGTCGTCGACGGAATTACGGCCTAAGCTGACGAAGCTGCTCCTCAACGTTACGATCCAGGGGAGCGTTGGGGCCGTACAGGTTGTGATGTCGCCGGAATCAAAGGTCGGAGATCTGGTCGCGGCGGCCGTGCGGCAGTACGGCAAGGAAGGTCGCCGTCCAATCTTGCCTTCCGTTGAGCCGTCGTTTTTCGACCTACATTACTCGCAGTTCAGCTTAGAAAGTTTGGATAGGGAAAAGAAGCTGATGGAGTTGGGATCCAGGAACTTTTTTCTATGCCCAAACGCAATAGCGGTGGGCGGTGGCGGCTGCGGGGGAGCGACGGCGTCGTCCGCTTCGTGTTCGAAAGAGGCGGAGAAAGTTTCACGAACTGGGTTTGCGTGGCTCAAGTTCATGGATTTCTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

198

Amino Acids

21.39

Weight (kDa)

10.01

Isoelectric Point (pI)

63.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 70 - 154 8.4e-38 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 511
AccB1I GGYRCC 2 cut(s) 115, 142
AccBSI CCGCTC 1 cut(s) 67
AccI GTMKAC 1 cut(s) 191
AccII CGCG 1 cut(s) 305
AclI AACGTT 1 cut(s) 231
AclWI GGATC 3 cut(s) 232, 435, 448
AcoI YGGCCR 2 cut(s) 94, 309
AcyI GRCGYC 3 cut(s) 116, 155, 509
AfaI GTAC 2 cut(s) 261, 323
AfiI CCNNNNNNNGG 1 cut(s) 103
AjnI CCWGG 2 cut(s) 240, 443
AjuI GAANNNNNNNTTGG 2 cut(s) 337, 369
AluBI AGCT 5 cut(s) 124, 211, 220, 399, 427
AluI AGCT 5 cut(s) 124, 211, 220, 399, 427
Alw26I GTCTC 1 cut(s) 55
AlwI GGATC 3 cut(s) 232, 435, 448
AoxI GGCC 6 cut(s) 56, 94, 131, 203, 255, 309
ApeKI GCWGC 4 cut(s) 34, 121, 220, 492
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 132, 255
AsuII TTCGAA 1 cut(s) 527
BamHI GGATCC 1 cut(s) 440
BanI GGYRCC 2 cut(s) 115, 142
BanII GRGCYC 1 cut(s) 11
BbvI GCAGC 4 cut(s) 46, 133, 207, 479
BccI CCATC 1 cut(s) 424
BceAI ACGGC 8 cut(s) 109, 218, 242, 296, 324, 340, 349, 522
BciT130I CCWGG 2 cut(s) 242, 445
BcoDI GTCTC 1 cut(s) 55
BfaI CTAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 119
BglII AGATCT 1 cut(s) 295
Bme1390I CCNGG 2 cut(s) 242, 445
BmgT120I GGNCC 2 cut(s) 132, 255
BmiI GGNNCC 4 cut(s) 117, 144, 256, 442
BmrFI CCNGG 2 cut(s) 242, 445
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 1 cut(s) 97
BmuI ACTGGG 1 cut(s) 564
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 527
BpuEI CTTGAG 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 82
BsaHI GRCGYC 3 cut(s) 116, 155, 509
BsaI GGTCTC 1 cut(s) 55
BsaJI CCNNGG 2 cut(s) 91, 241
BsaXI ACNNNNNCTCC 2 cut(s) 285, 315
Bsc4I CCNNNNNNNGG 1 cut(s) 103
Bse1I ACTGG 2 cut(s) 97, 559
Bse8I GATNNNNATC 1 cut(s) 82
BseBI CCWGG 2 cut(s) 242, 445
BseDI CCNNGG 2 cut(s) 91, 241
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 1 cut(s) 103
BseNI ACTGG 2 cut(s) 97, 559
BseRI GAGGAG 1 cut(s) 215
BseX3I CGGCCG 1 cut(s) 309
BseXI GCAGC 4 cut(s) 46, 133, 207, 479
Bsh1236I CGCG 1 cut(s) 305
Bsh1285I CGRYCG 2 cut(s) 80, 312
BshFI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BshNI GGYRCC 2 cut(s) 115, 142
BsiEI CGRYCG 2 cut(s) 80, 312
BsiSI CCGG 4 cut(s) 25, 38, 146, 278
BslI CCNNNNNNNGG 1 cut(s) 103
BsmAI GTCTC 1 cut(s) 55
BsnI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
Bso31I GGTCTC 1 cut(s) 55
Bsp119I TTCGAA 1 cut(s) 527
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 4 cut(s) 77, 237, 295, 440
BspANI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 4 cut(s) 117, 144, 256, 442
BspPI GGATC 3 cut(s) 232, 435, 448
BspT104I TTCGAA 1 cut(s) 527
BspT107I GGYRCC 2 cut(s) 115, 142
BspTNI GGTCTC 1 cut(s) 55
BsrBI CCGCTC 1 cut(s) 67
BsrI ACTGG 2 cut(s) 97, 559
BssECI CCNNGG 2 cut(s) 91, 241
BssMI GATC 4 cut(s) 77, 237, 295, 440
BssNI GRCGYC 3 cut(s) 116, 155, 509
Bst2UI CCWGG 2 cut(s) 242, 445
Bst4CI ACNGT 2 cut(s) 142, 184
Bst6I CTCTTC 1 cut(s) 36
BstACI GRCGYC 3 cut(s) 116, 155, 509
BstBI TTCGAA 1 cut(s) 527
BstC8I GCNNGC 1 cut(s) 56
BstDEI CTNAG 2 cut(s) 207, 400
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 112
BstFNI CGCG 1 cut(s) 305
BstH2I RGCGCY 1 cut(s) 119
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 4 cut(s) 80, 240, 298, 443
BstMAI GTCTC 1 cut(s) 55
BstMBI GATC 4 cut(s) 77, 237, 295, 440
BstMCI CGRYCG 2 cut(s) 80, 312
BstMWI GCNNNNNNNGC 5 cut(s) 64, 130, 217, 489, 515
BstNI CCWGG 2 cut(s) 242, 445
BstSCI CCNGG 2 cut(s) 240, 443
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 4 cut(s) 46, 133, 207, 479
BstX2I RGATCY 2 cut(s) 295, 440
BstYI RGATCY 2 cut(s) 295, 440
BstZI CGGCCG 1 cut(s) 309
BsuRI GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
BtgI CCRYGG 1 cut(s) 91
BtsCI GGATG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 56
CfoI GCGC 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 132, 255
CseI GACGC 2 cut(s) 144, 498
Csp6I GTAC 2 cut(s) 260, 322
CspCI CAANNNNNGTGG 2 cut(s) 459, 494
CviAII CATG 1 cut(s) 577
CviQI GTAC 2 cut(s) 260, 322
DdeI CTNAG 2 cut(s) 207, 400
DinI GGCGCC 1 cut(s) 117
DpnI GATC 4 cut(s) 79, 239, 297, 442
DpnII GATC 4 cut(s) 77, 237, 295, 440
DrdI GACNNNNNNGTC 1 cut(s) 511
DseDI GACNNNNNNGTC 1 cut(s) 511
EaeI YGGCCR 2 cut(s) 94, 309
EagI CGGCCG 1 cut(s) 309
Eam1104I CTCTTC 1 cut(s) 36
EarI CTCTTC 1 cut(s) 36
EciI GGCGGA 1 cut(s) 551
EclXI CGGCCG 1 cut(s) 309
Eco24I GRGCYC 1 cut(s) 11
Eco31I GGTCTC 1 cut(s) 55
Eco52I CGGCCG 1 cut(s) 309
EcoRII CCWGG 2 cut(s) 240, 443
EcoT38I GRGCYC 1 cut(s) 11
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
FaeI CATG 1 cut(s) 580
FaiI YATR 3 cut(s) 152, 460, 578
FatI CATG 1 cut(s) 576
FauI CCCGC 1 cut(s) 488
FblI GTMKAC 1 cut(s) 191
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 11
FspBI CTAG 1 cut(s) 21
GlaI GCGC 1 cut(s) 117
HaeII RGCGCY 1 cut(s) 119
HaeIII GGCC 6 cut(s) 58, 96, 133, 205, 257, 311
HapII CCGG 4 cut(s) 25, 38, 146, 278
HgaI GACGC 2 cut(s) 144, 498
HhaI GCGC 1 cut(s) 118
Hin1I GRCGYC 3 cut(s) 116, 155, 509
Hin1II CATG 1 cut(s) 580
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 192
HindII GTYRAC 1 cut(s) 192
HinfI GANTC 1 cut(s) 281
HpaII CCGG 4 cut(s) 25, 38, 146, 278
Hpy166II GTNNAC 2 cut(s) 102, 192
Hpy188I TCNGA 3 cut(s) 129, 176, 293
Hpy188III TCNNGA 2 cut(s) 163, 549
Hpy8I GTNNAC 2 cut(s) 102, 192
Hpy99I CGWCG 6 cut(s) 160, 193, 196, 370, 508, 514
HpyAV CCTTC 2 cut(s) 326, 363
HpyCH4III ACNGT 2 cut(s) 142, 184
HpyCH4IV ACGT 1 cut(s) 231
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 130, 217, 489, 515
HpyF3I CTNAG 2 cut(s) 207, 400
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 3 cut(s) 116, 155, 509
Hsp92II CATG 1 cut(s) 580
HspAI GCGC 1 cut(s) 116
KasI GGCGCC 1 cut(s) 115
Kzo9I GATC 4 cut(s) 77, 237, 295, 440
LmnI GCTCC 3 cut(s) 228, 246, 500
Lsp1109I GCAGC 4 cut(s) 46, 133, 207, 479
LweI GCATC 1 cut(s) 97
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 232
MalI GATC 4 cut(s) 79, 239, 297, 442
MbiI CCGCTC 1 cut(s) 67
MboI GATC 4 cut(s) 77, 237, 295, 440
MboII GAAGA 3 cut(s) 25, 53, 58
MflI RGATCY 2 cut(s) 295, 440
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 1 cut(s) 197
Mly113I GGCGCC 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 154, 271
MnlI CCTC 4 cut(s) 21, 123, 236, 526
MspI CCGG 4 cut(s) 25, 38, 146, 278
MspR9I CCNGG 2 cut(s) 242, 445
MvaI CCWGG 2 cut(s) 242, 445
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 5 cut(s) 64, 130, 217, 489, 515
NarI GGCGCC 1 cut(s) 116
NdeII GATC 4 cut(s) 77, 237, 295, 440
NlaIII CATG 1 cut(s) 580
NlaIV GGNNCC 4 cut(s) 117, 144, 256, 442
NmeAIII GCCGAG 2 cut(s) 30, 84
NspV TTCGAA 1 cut(s) 527
PcsI WCGNNNNNNNCGW 3 cut(s) 188, 309, 518
PfeI GAWTC 1 cut(s) 281
PflFI GACNNNGTC 1 cut(s) 508
PfoI TCCNGGA 1 cut(s) 443
Ple19I CGATCG 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 119
Psp1406I AACGTT 1 cut(s) 231
Psp6I CCWGG 2 cut(s) 240, 443
PspGI CCWGG 2 cut(s) 240, 443
PspN4I GGNNCC 4 cut(s) 117, 144, 256, 442
PspPI GGNCC 2 cut(s) 132, 255
PsuI RGATCY 2 cut(s) 295, 440
PsyI GACNNNGTC 1 cut(s) 508
PvuI CGATCG 1 cut(s) 80
RsaI GTAC 2 cut(s) 261, 323
RsaNI GTAC 2 cut(s) 260, 322
SalI GTCGAC 1 cut(s) 190
Sau3AI GATC 4 cut(s) 77, 237, 295, 440
Sau96I GGNCC 2 cut(s) 132, 255
ScrFI CCNGG 2 cut(s) 242, 445
SduI GDGCHC 1 cut(s) 11
SfaNI GCATC 1 cut(s) 97
SfoI GGCGCC 1 cut(s) 117
SfuI TTCGAA 1 cut(s) 527
SgrDI CGTCGACG 1 cut(s) 190
SmlI CTYRAG 1 cut(s) 569
SmoI CTYRAG 1 cut(s) 569
Sse9I AATT 1 cut(s) 197
SspDI GGCGCC 1 cut(s) 115
SspMI CTAG 1 cut(s) 21
StyD4I CCNGG 2 cut(s) 240, 443
TaaI ACNGT 2 cut(s) 142, 184
TaiI ACGT 1 cut(s) 234
TaqI TCGA 4 cut(s) 76, 191, 375, 527
TasI AATT 1 cut(s) 197
TauI GCSGC 7 cut(s) 113, 116, 121, 308, 311, 319, 492
TfiI GAWTC 1 cut(s) 281
TseI GCWGC 4 cut(s) 34, 121, 220, 492
TspDTI ATGAA 1 cut(s) 565
TspGWI ACGGA 3 cut(s) 119, 209, 346
Tth111I GACNNNGTC 1 cut(s) 508
XcmI CCANNNNNNNNNTGG 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 191
XspI CTAG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.