RLG00000034981

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0010479
Physical Location & Seq
Forward (+)
58269738 .. 58270430
693 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034981

Sequence Viewer

Length: 585 bp
ATGCAAGAGGAGCACAAGAACCGGAGGGGCAGATTACTCTCGGAGAGATCCTCGTCGTTTCACGGCCAGATGTTGTCCGCGTCGTCGACGGCGACACCGCAGCTCCGGCGGCCGAAGACGGTCCCGGACATGGTCCTGTACAGAAACGCTGTCGGAGCCACTATGCCGCCGACGGCGTTGCCGGAGCTGCGGCCTAAGCTGACGAAGCTGCTGCTGAACGTGACGATACAAGGGAGCGTCGGTGCCGTACAGGTGGTTATGTCGCCGGAATCGACTGTCGGAGATCTTGTGGCGGCGGCCGTACGGCAGTACGAGAAAGAAGGTCGCCGGCCGATATTCCCCTCCGTCGATCCCTCACGCTTCGACCTCCATTATTCCCAGTTCAGCTTAGAAAGTTTGGATAGGGAGGAGAAGCTGATGGAGTTGGGGTCGCGCAACTTCTTCCTGTGCCCTAAGAAGTCGGCAGCGGACGGTGGGAGTAGCGGTGGCGGGATAACGACGTCGTCTGGTTCATGTTCGAAACAGGCGGAGAAAGCTTCCAAAACTGGGTTTTCTTGGCTCAAGTTTATGGATTCCCTGCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

21.19

Weight (kDa)

9.61

Isoelectric Point (pI)

58.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 66 - 150 3.6e-39 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 242
AccI GTMKAC 1 cut(s) 86
AccII CGCG 2 cut(s) 80, 433
AclWI GGATC 2 cut(s) 42, 344
AcoI YGGCCR 4 cut(s) 64, 110, 297, 329
AcyI GRCGYC 1 cut(s) 500
AfaI GTAC 4 cut(s) 140, 249, 303, 311
AfiI CCNNNNNNNGG 2 cut(s) 130, 546
AluBI AGCT 7 cut(s) 103, 187, 199, 208, 387, 415, 536
AluI AGCT 7 cut(s) 103, 187, 199, 208, 387, 415, 536
Alw21I GWGCWC 1 cut(s) 15
AlwI GGATC 2 cut(s) 42, 344
AoxI GGCC 5 cut(s) 64, 110, 191, 297, 329
ApeKI GCWGC 5 cut(s) 100, 187, 208, 211, 464
AspLEI GCGC 1 cut(s) 435
AspS9I GGNCC 2 cut(s) 121, 133
AsuC2I CCSGG 1 cut(s) 125
AsuII TTCGAA 1 cut(s) 518
AvaII GGWCC 2 cut(s) 121, 133
BaeGI GKGCMC 1 cut(s) 452
BanI GGYRCC 1 cut(s) 242
BbsI GAAGAC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 15
BbvI GCAGC 5 cut(s) 112, 174, 195, 198, 476
BccI CCATC 1 cut(s) 412
BceAI ACGGC 6 cut(s) 79, 105, 189, 230, 284, 320
BcgI CGANNNNNNTGC 4 cut(s) 160, 193, 194, 227
BcnI CCSGG 1 cut(s) 125
BglII AGATCT 1 cut(s) 283
Bme1390I CCNGG 1 cut(s) 125
Bme18I GGWCC 2 cut(s) 121, 133
BmgT120I GGNCC 2 cut(s) 121, 133
BmiI GGNNCC 3 cut(s) 123, 157, 244
BmrFI CCNGG 1 cut(s) 125
BmrI ACTGGG 2 cut(s) 373, 555
BmuI ACTGGG 2 cut(s) 373, 555
BpiI GAAGAC 1 cut(s) 122
BplI GAGNNNNNCTC 2 cut(s) 35, 67
Bpu10I CCTNAGC 1 cut(s) 195
Bpu14I TTCGAA 1 cut(s) 518
BpuEI CTTGAG 1 cut(s) 545
BpuMI CCSGG 1 cut(s) 125
BsaHI GRCGYC 1 cut(s) 500
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 2 cut(s) 130, 546
Bse118I RCCGGY 1 cut(s) 327
Bse1I ACTGG 2 cut(s) 379, 550
BseLI CCNNNNNNNGG 2 cut(s) 130, 546
BseNI ACTGG 2 cut(s) 379, 550
BseRI GAGGAG 2 cut(s) 23, 422
BseSI GKGCMC 1 cut(s) 452
BseX3I CGGCCG 3 cut(s) 110, 297, 329
BseXI GCAGC 5 cut(s) 112, 174, 195, 198, 476
Bsh1236I CGCG 2 cut(s) 80, 433
Bsh1285I CGRYCG 3 cut(s) 113, 300, 332
BshFI GGCC 5 cut(s) 66, 112, 193, 299, 331
BshNI GGYRCC 1 cut(s) 242
BsiEI CGRYCG 3 cut(s) 113, 300, 332
BsiHKAI GWGCWC 1 cut(s) 15
BsiSI CCGG 6 cut(s) 22, 106, 125, 182, 266, 328
BsiWI CGTACG 1 cut(s) 301
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 2 cut(s) 130, 546
BsmFI GGGAC 1 cut(s) 107
BsnI GGCC 5 cut(s) 66, 112, 193, 299, 331
Bsp119I TTCGAA 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 15, 452
Bsp1407I TGTACA 1 cut(s) 138
Bsp143I GATC 3 cut(s) 47, 283, 349
BspANI GGCC 5 cut(s) 66, 112, 193, 299, 331
BspFNI CGCG 2 cut(s) 80, 433
BspLI GGNNCC 3 cut(s) 123, 157, 244
BspPI GGATC 2 cut(s) 42, 344
BspT104I TTCGAA 1 cut(s) 518
BspT107I GGYRCC 1 cut(s) 242
BsrFI RCCGGY 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 138
BsrI ACTGG 2 cut(s) 379, 550
BssAI RCCGGY 1 cut(s) 327
BssMI GATC 3 cut(s) 47, 283, 349
BssNI GRCGYC 1 cut(s) 500
Bst4CI ACNGT 3 cut(s) 121, 277, 473
BstACI GRCGYC 1 cut(s) 500
BstAUI TGTACA 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 518
BstC8I GCNNGC 1 cut(s) 329
BstDEI CTNAG 3 cut(s) 195, 388, 453
BstFNI CGCG 2 cut(s) 80, 433
BstHHI GCGC 1 cut(s) 435
BstKTI GATC 3 cut(s) 50, 286, 352
BstMBI GATC 3 cut(s) 47, 283, 349
BstMCI CGRYCG 3 cut(s) 113, 300, 332
BstMWI GCNNNNNNNGC 8 cut(s) 10, 106, 109, 155, 187, 196, 205, 533
BstSCI CCNGG 1 cut(s) 123
BstSLI GKGCMC 1 cut(s) 452
BstUI CGCG 2 cut(s) 80, 433
BstV1I GCAGC 5 cut(s) 112, 174, 195, 198, 476
BstV2I GAAGAC 1 cut(s) 122
BstX2I RGATCY 2 cut(s) 47, 283
BstYI RGATCY 2 cut(s) 47, 283
BstZI CGGCCG 3 cut(s) 110, 297, 329
BsuRI GGCC 5 cut(s) 66, 112, 193, 299, 331
Cac8I GCNNGC 1 cut(s) 329
CfoI GCGC 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 121, 133
CseI GACGC 2 cut(s) 69, 226
Csp6I GTAC 4 cut(s) 139, 248, 302, 310
CviAII CATG 2 cut(s) 130, 513
CviQI GTAC 4 cut(s) 139, 248, 302, 310
DdeI CTNAG 3 cut(s) 195, 388, 453
DpnI GATC 3 cut(s) 49, 285, 351
DpnII GATC 3 cut(s) 47, 283, 349
EaeI YGGCCR 4 cut(s) 64, 110, 297, 329
EagI CGGCCG 3 cut(s) 110, 297, 329
EciI GGCGGA 1 cut(s) 542
EclXI CGGCCG 3 cut(s) 110, 297, 329
Eco47I GGWCC 2 cut(s) 121, 133
Eco52I CGGCCG 3 cut(s) 110, 297, 329
FaeI CATG 2 cut(s) 133, 516
FaiI YATR 5 cut(s) 131, 164, 260, 514, 569
FaqI GGGAC 1 cut(s) 107
FatI CATG 2 cut(s) 129, 512
FauI CCCGC 1 cut(s) 482
FblI GTMKAC 1 cut(s) 86
GlaI GCGC 1 cut(s) 434
HaeIII GGCC 5 cut(s) 66, 112, 193, 299, 331
HapII CCGG 6 cut(s) 22, 106, 125, 182, 266, 328
HgaI GACGC 2 cut(s) 69, 226
HhaI GCGC 1 cut(s) 435
Hin1I GRCGYC 1 cut(s) 500
Hin1II CATG 2 cut(s) 133, 516
Hin6I GCGC 1 cut(s) 433
HinP1I GCGC 1 cut(s) 433
HincII GTYRAC 1 cut(s) 87
HindII GTYRAC 1 cut(s) 87
HindIII AAGCTT 1 cut(s) 534
HinfI GANTC 2 cut(s) 269, 572
HpaII CCGG 6 cut(s) 22, 106, 125, 182, 266, 328
Hpy166II GTNNAC 1 cut(s) 87
Hpy188I TCNGA 3 cut(s) 43, 155, 281
Hpy8I GTNNAC 1 cut(s) 87
Hpy99I CGWCG 9 cut(s) 58, 85, 88, 91, 175, 242, 350, 502, 505
HpyAV CCTTC 1 cut(s) 314
HpyCH4III ACNGT 3 cut(s) 121, 277, 473
HpyCH4IV ACGT 2 cut(s) 219, 500
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 106, 109, 155, 187, 196, 205, 533
HpyF3I CTNAG 3 cut(s) 195, 388, 453
HpySE526I ACGT 2 cut(s) 219, 500
Hsp92I GRCGYC 1 cut(s) 500
Hsp92II CATG 2 cut(s) 133, 516
HspAI GCGC 1 cut(s) 433
KroI GCCGGC 1 cut(s) 327
KroNI GCCGGC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 47, 283, 349
LmnI GCTCC 5 cut(s) 10, 108, 155, 184, 234
Lsp1109I GCAGC 5 cut(s) 112, 174, 195, 198, 476
MaeII ACGT 2 cut(s) 219, 500
MaeIII GTNAC 1 cut(s) 220
MalI GATC 3 cut(s) 49, 285, 351
MboI GATC 3 cut(s) 47, 283, 349
MboII GAAGA 2 cut(s) 127, 433
MflI RGATCY 2 cut(s) 47, 283
MhlI GDGCHC 2 cut(s) 15, 452
MmeI TCCRAC 2 cut(s) 133, 259
MnlI CCTC 6 cut(s) 18, 61, 352, 364, 377, 400
MroNI GCCGGC 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 6 cut(s) 22, 106, 125, 182, 266, 328
MspR9I CCNGG 1 cut(s) 125
MvnI CGCG 2 cut(s) 80, 433
MwoI GCNNNNNNNGC 8 cut(s) 10, 106, 109, 155, 187, 196, 205, 533
NaeI GCCGGC 1 cut(s) 329
NciI CCSGG 1 cut(s) 125
NdeII GATC 3 cut(s) 47, 283, 349
NgoMIV GCCGGC 1 cut(s) 327
NlaIII CATG 2 cut(s) 133, 516
NlaIV GGNNCC 3 cut(s) 123, 157, 244
NmuCI GTSAC 1 cut(s) 220
NspV TTCGAA 1 cut(s) 518
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 269
PdiI GCCGGC 1 cut(s) 329
PfeI GAWTC 2 cut(s) 269, 572
Pfl23II CGTACG 1 cut(s) 301
PflFI GACNNNGTC 2 cut(s) 131, 502
PfoI TCCNGGA 1 cut(s) 123
PspLI CGTACG 1 cut(s) 301
PspN4I GGNNCC 3 cut(s) 123, 157, 244
PspPI GGNCC 2 cut(s) 121, 133
PsuI RGATCY 2 cut(s) 47, 283
PsyI GACNNNGTC 2 cut(s) 131, 502
RsaI GTAC 4 cut(s) 140, 249, 303, 311
RsaNI GTAC 4 cut(s) 139, 248, 302, 310
SalI GTCGAC 1 cut(s) 85
Sau3AI GATC 3 cut(s) 47, 283, 349
Sau96I GGNCC 2 cut(s) 121, 133
ScrFI CCNGG 1 cut(s) 125
SduI GDGCHC 2 cut(s) 15, 452
SfuI TTCGAA 1 cut(s) 518
SgrDI CGTCGACG 1 cut(s) 85
SinI GGWCC 2 cut(s) 121, 133
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
StyD4I CCNGG 1 cut(s) 123
TaaI ACNGT 3 cut(s) 121, 277, 473
TaiI ACGT 2 cut(s) 222, 503
TaqI TCGA 5 cut(s) 86, 272, 348, 363, 518
TatI WGTACW 1 cut(s) 138
TauI GCSGC 5 cut(s) 112, 169, 193, 296, 299
TfiI GAWTC 2 cut(s) 269, 572
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 5 cut(s) 100, 187, 208, 211, 464
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 501
TspGWI ACGGA 1 cut(s) 334
Tth111I GACNNNGTC 2 cut(s) 131, 502
VpaK11BI GGWCC 2 cut(s) 121, 133
XmiI GTMKAC 1 cut(s) 86
ZraI GACGTC 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.