FvH4_3g30460

Belongs to the histone H2B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0017481
Physical Location & Seq
Forward (+)
23733698 .. 23736188
2491 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g30460.t1

Sequence Viewer

Length: 1239 bp
ATGGTTTGTATCGTGGCAATAGACGCACCAAAGTTTATCAGAAGCCAGTTTTGCTTACAATTTCACTTTTACCACCACGTAGCCATTATGTTAAGCAACCACTCAACACCTGTCCACCACATGTCGACCTGTAGCAAACCTCCCTACACGAACAGTTATTATCTCCTCAATCCTGCTGTAACTTCAGTCTATATCAATAACAATATCACCGCACCGTTAGCCAACTGTTTTGTCAGGAAATTAACGTCGATTATAATGAGTCCGAAGCGCCGGTCATCATCCAGGTTGGTGGTGAAGACCACCAAGCAAGTTGTGAAGGAGACGGTTGAAGTCTCGGTGGTGCGGAGTAAGAAGAGGCAGAAGAAAGTGAATGATGATGAACAACAAGAGCCAGTCGAGAGTACTATTGCCTTCGAAACCAAAAAAGAAAACCAGACAGAGAAAATAGAAGTCTCGGTTGAGAAAGATCCTGCCAAGAGAGCTATTGAAAGCCAAGAAGAAACTCAAATTGCTGAAGAAGACAAAGAGCCAGAGGAAATAAGGACTATTCCTGTAGAGGTCCCCGGCATGGAAACCCCAGAGAAACTAAGAGGAGCAACTCTGAGCGTTGACAAAGACGAAGAGCCTAAAGAACCAAGCACTGTTCTAGATGACAAAGAGCCAGTCAGCAATGATGTTGTCACGGAAACTCAACTAGACAGTGATGAAACTCAAAACTTTGAAGAAGACGGAGAGCAGGCCAGGACTATTTCCATGGATAGCCAAGAAGGAAGTGAAACACAGAATTTTGAAGAGACCCCTATGACACCTGAAAAGAAAGAAGTAAACCAATCATCCAAGGTTGAAAAGGAGAAGAGTGATGACGTCAAGCAAACCGGAGATGGAGAGAAGAAAGACAAGAAGAGGAAGAGGAGATCACCAGGGAATAAAAGAGAGGGAGGAAAAGGGTATAAGAGGTATGTGTATAAAGTGTTCAAGCAGGTGCACCCTGAACTGGGCATGTCAGCAAAGGCCATGGTGGTTCTCAACAATTATATGAATGACATGTTCGAGAGGCTAGCTGGTGAGGCAGCAAAGCTCACCATGTACACGTCTCGAAAGACACTGTCTTCGAGGGAGATTCAAGGGGCAGTGAAGCTGGTTTTGCCTGGGGAGCTTGGGAAACATGCCATGGCAGAAGGGACCAAGGCTGTGAGCAACTACTTGTCCAAGAACGCAGCGATGTCCCATAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

413

Amino Acids

46.5

Weight (kDa)

6.79

Isoelectric Point (pI)

57.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 300 - 380 4.8e-17 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08170
fragaria_vesca FvH4_3g30460
malus_domestica MD11G1150000.v1.1
prunus_persica Prupe.6G114300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0056411
rosa_laevigata RLG00000035096
rosa_multiflora Rmu_sc0000317.1_g000038
rosa_rugosa Rorug05G0299300
rosa_samantha Rh5AG370200 Rh5BG381400 Rh5BG381600
rosa_wichuraiana Rw5G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 254
AarI CACCTGC 1 cut(s) 970
AatII GACGTC 1 cut(s) 867
Acc36I ACCTGC 1 cut(s) 970
AccI GTMKAC 1 cut(s) 125
AciI CCGC 2 cut(s) 210, 343
AclWI GGATC 1 cut(s) 461
AcsI RAATTY 1 cut(s) 784
AcuI CTGAAG 2 cut(s) 168, 534
AcyI GRCGYC 1 cut(s) 864
AfaI GTAC 2 cut(s) 403, 1088
AfiI CCNNNNNNNGG 3 cut(s) 568, 994, 995
AflIII ACRYGT 3 cut(s) 120, 1044, 1089
AgsI TTSAA 7 cut(s) 329, 488, 722, 791, 845, 976, 1124
AjiI CACGTC 1 cut(s) 1092
AjnI CCWGG 4 cut(s) 281, 740, 919, 1147
AluBI AGCT 5 cut(s) 482, 1061, 1078, 1138, 1156
AluI AGCT 5 cut(s) 482, 1061, 1078, 1138, 1156
Alw21I GWGCWC 1 cut(s) 987
Alw26I GTCTC 5 cut(s) 314, 337, 457, 788, 1098
Alw44I GTGCAC 1 cut(s) 983
AlwI GGATC 1 cut(s) 461
AoxI GGCC 2 cut(s) 738, 1011
ApaLI GTGCAC 1 cut(s) 983
ApeKI GCWGC 2 cut(s) 1070, 1217
ApoI RAATTY 1 cut(s) 784
ArsI GACNNNNNNTTYG 6 cut(s) 257, 289, 648, 680, 1093, 1125
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 2 cut(s) 559, 1182
AsuC2I CCSGG 1 cut(s) 564
AsuHPI GGTGA 5 cut(s) 199, 304, 909, 1072, 1076
AsuII TTCGAA 1 cut(s) 414
AsuNHI GCTAGC 1 cut(s) 1057
AvaII GGWCC 2 cut(s) 559, 1182
BaeGI GKGCMC 1 cut(s) 987
BbsI GAAGAC 4 cut(s) 302, 525, 732, 1101
Bbv12I GWGCWC 1 cut(s) 987
BbvI GCAGC 2 cut(s) 1082, 1229
BccI CCATC 1 cut(s) 875
BciT130I CCWGG 4 cut(s) 283, 742, 921, 1149
BcnI CCSGG 1 cut(s) 564
BcoDI GTCTC 5 cut(s) 314, 337, 457, 788, 1098
BfaI CTAG 3 cut(s) 647, 695, 1058
BfmI CTRYAG 2 cut(s) 130, 552
BfoI RGCGCY 1 cut(s) 271
BfuAI ACCTGC 1 cut(s) 970
BisI GCNGC 2 cut(s) 1071, 1218
BlsI GCNGC 2 cut(s) 1072, 1219
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 5 cut(s) 283, 564, 742, 921, 1149
Bme18I GGWCC 2 cut(s) 559, 1182
BmgBI CACGTC 1 cut(s) 1092
BmgT120I GGNCC 2 cut(s) 559, 1182
BmiI GGNNCC 2 cut(s) 561, 1183
BmrFI CCNGG 5 cut(s) 283, 564, 742, 921, 1149
BmrI ACTGGG 1 cut(s) 1004
BmtI GCTAGC 1 cut(s) 1061
BmuI ACTGGG 1 cut(s) 1004
BpiI GAAGAC 4 cut(s) 302, 525, 732, 1101
Bpu14I TTCGAA 1 cut(s) 414
BpuMI CCSGG 1 cut(s) 564
BsaAI YACGTR 1 cut(s) 79
BsaHI GRCGYC 1 cut(s) 864
BsaI GGTCTC 1 cut(s) 788
BsaJI CCNNGG 8 cut(s) 562, 753, 837, 920, 1014, 1148, 1170, 1185
BsaWI WCCGGW 1 cut(s) 875
Bsc4I CCNNNNNNNGG 3 cut(s) 568, 994, 995
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 4 cut(s) 46, 392, 662, 999
Bse3DI GCAATG 1 cut(s) 676
BseBI CCWGG 4 cut(s) 283, 742, 921, 1149
BseDI CCNNGG 8 cut(s) 562, 753, 837, 920, 1014, 1148, 1170, 1185
BseGI GGATG 2 cut(s) 278, 833
BseLI CCNNNNNNNGG 3 cut(s) 568, 994, 995
BseMI GCAATG 1 cut(s) 676
BseMII CTCAG 1 cut(s) 593
BseNI ACTGG 4 cut(s) 46, 392, 662, 999
BseRI GAGGAG 3 cut(s) 155, 606, 925
BseSI GKGCMC 1 cut(s) 987
BseXI GCAGC 2 cut(s) 1082, 1229
BshFI GGCC 2 cut(s) 740, 1013
BsiHKAI GWGCWC 1 cut(s) 987
BsiSI CCGG 3 cut(s) 271, 564, 876
BslFI GGGAC 3 cut(s) 545, 1195, 1210
BslI CCNNNNNNNGG 3 cut(s) 568, 994, 995
BsmAI GTCTC 5 cut(s) 314, 337, 457, 788, 1098
BsmBI CGTCTC 2 cut(s) 314, 1098
BsmFI GGGAC 3 cut(s) 545, 1195, 1210
BsnI GGCC 2 cut(s) 740, 1013
Bso31I GGTCTC 1 cut(s) 788
Bsp119I TTCGAA 1 cut(s) 414
Bsp1286I GDGCHC 1 cut(s) 987
Bsp1407I TGTACA 1 cut(s) 1086
Bsp143I GATC 2 cut(s) 466, 914
Bsp19I CCATGG 3 cut(s) 753, 1014, 1170
BspACI CCGC 2 cut(s) 210, 343
BspANI GGCC 2 cut(s) 740, 1013
BspCNI CTCAG 1 cut(s) 594
BspLI GGNNCC 2 cut(s) 561, 1183
BspMI ACCTGC 1 cut(s) 970
BspOI GCTAGC 1 cut(s) 1061
BspPI GGATC 1 cut(s) 461
BspQI GCTCTTC 1 cut(s) 615
BspT104I TTCGAA 1 cut(s) 414
BspTNI GGTCTC 1 cut(s) 788
BsrDI GCAATG 1 cut(s) 676
BsrFI RCCGGY 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 1086
BsrI ACTGG 4 cut(s) 46, 392, 662, 999
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 8 cut(s) 562, 753, 837, 920, 1014, 1148, 1170, 1185
BssMI GATC 2 cut(s) 466, 914
BssNI GRCGYC 1 cut(s) 864
BssT1I CCWWGG 5 cut(s) 753, 837, 1014, 1170, 1185
Bst2UI CCWGG 4 cut(s) 283, 742, 921, 1149
Bst4CI ACNGT 7 cut(s) 155, 216, 227, 325, 643, 701, 1107
Bst6I CTCTTC 6 cut(s) 347, 615, 786, 848, 896, 902
BstACI GRCGYC 1 cut(s) 864
BstAUI TGTACA 1 cut(s) 1086
BstBAI YACGTR 1 cut(s) 79
BstBI TTCGAA 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 738, 1059
BstDEI CTNAG 2 cut(s) 587, 602
BstDSI CCRYGG 3 cut(s) 753, 1014, 1170
BstF5I GGATG 2 cut(s) 278, 833
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 2 cut(s) 469, 917
BstMAI GTCTC 5 cut(s) 314, 337, 457, 788, 1098
BstMBI GATC 2 cut(s) 466, 914
BstMWI GCNNNNNNNGC 7 cut(s) 23, 51, 218, 479, 1067, 1144, 1153
BstNI CCWGG 4 cut(s) 283, 742, 921, 1149
BstNSI RCATGY 4 cut(s) 124, 1003, 1048, 1169
BstSCI CCNGG 5 cut(s) 281, 562, 740, 919, 1147
BstSFI CTRYAG 2 cut(s) 130, 552
BstSLI GKGCMC 1 cut(s) 987
BstV1I GCAGC 2 cut(s) 1082, 1229
BstV2I GAAGAC 4 cut(s) 302, 525, 732, 1101
BstX2I RGATCY 1 cut(s) 466
BstXI CCANNNNNNTGG 1 cut(s) 289
BstYI RGATCY 1 cut(s) 466
BsuRI GGCC 2 cut(s) 740, 1013
BtgI CCRYGG 3 cut(s) 753, 1014, 1170
BtgZI GCGATG 1 cut(s) 1235
BtrI CACGTC 1 cut(s) 1092
BtsCI GGATG 2 cut(s) 278, 833
BtsI GCAGTG 1 cut(s) 1137
BtsIMutI CAGTG 4 cut(s) 639, 706, 1103, 1137
BveI ACCTGC 1 cut(s) 970
Cac8I GCNNGC 2 cut(s) 738, 1059
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 2 cut(s) 559, 1182
CseI GACGC 1 cut(s) 32
Csp6I GTAC 2 cut(s) 402, 1087
CviAII CATG 9 cut(s) 121, 568, 754, 1000, 1015, 1045, 1084, 1166, 1171
CviQI GTAC 2 cut(s) 402, 1087
DdeI CTNAG 2 cut(s) 587, 602
DpnI GATC 2 cut(s) 468, 916
DpnII GATC 2 cut(s) 466, 914
Eam1104I CTCTTC 6 cut(s) 347, 615, 786, 848, 896, 902
EarI CTCTTC 6 cut(s) 347, 615, 786, 848, 896, 902
Eco130I CCWWGG 5 cut(s) 753, 837, 1014, 1170, 1185
Eco31I GGTCTC 1 cut(s) 788
Eco47I GGWCC 2 cut(s) 559, 1182
Eco57I CTGAAG 2 cut(s) 168, 534
EcoO109I RGGNCCY 1 cut(s) 559
EcoRII CCWGG 4 cut(s) 281, 740, 919, 1147
EcoT14I CCWWGG 5 cut(s) 753, 837, 1014, 1170, 1185
ErhI CCWWGG 5 cut(s) 753, 837, 1014, 1170, 1185
Esp3I CGTCTC 2 cut(s) 314, 1098
FaeI CATG 9 cut(s) 124, 571, 757, 1003, 1018, 1048, 1087, 1169, 1174
FaqI GGGAC 3 cut(s) 545, 1195, 1210
FatI CATG 9 cut(s) 120, 567, 753, 999, 1014, 1044, 1083, 1165, 1170
FblI GTMKAC 1 cut(s) 125
Fnu4HI GCNGC 2 cut(s) 1071, 1218
FokI GGATG 2 cut(s) 265, 820
Fsp4HI GCNGC 2 cut(s) 1071, 1218
FspBI CTAG 3 cut(s) 647, 695, 1058
GlaI GCGC 1 cut(s) 269
GluI GCNGC 2 cut(s) 1071, 1218
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 2 cut(s) 740, 1013
HapII CCGG 3 cut(s) 271, 564, 876
HgaI GACGC 1 cut(s) 32
HhaI GCGC 1 cut(s) 270
Hin1I GRCGYC 1 cut(s) 864
Hin1II CATG 9 cut(s) 124, 571, 757, 1003, 1018, 1048, 1087, 1169, 1174
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HincII GTYRAC 2 cut(s) 126, 610
HindII GTYRAC 2 cut(s) 126, 610
HinfI GANTC 2 cut(s) 259, 1120
HpaII CCGG 3 cut(s) 271, 564, 876
HphI GGTGA 5 cut(s) 199, 304, 909, 1072, 1076
Hpy166II GTNNAC 6 cut(s) 115, 126, 610, 826, 985, 1089
Hpy188I TCNGA 3 cut(s) 41, 264, 603
Hpy188III TCNNGA 6 cut(s) 235, 397, 647, 1051, 1095, 1236
Hpy8I GTNNAC 6 cut(s) 115, 126, 610, 826, 985, 1089
Hpy99I CGWCG 1 cut(s) 250
HpyAV CCTTC 4 cut(s) 310, 421, 761, 1172
HpyCH4III ACNGT 7 cut(s) 155, 216, 227, 325, 643, 701, 1107
HpyCH4IV ACGT 4 cut(s) 78, 245, 864, 1091
HpyCH4V TGCA 1 cut(s) 985
HpyF10VI GCNNNNNNNGC 7 cut(s) 23, 51, 218, 479, 1067, 1144, 1153
HpyF3I CTNAG 2 cut(s) 587, 602
HpySE526I ACGT 4 cut(s) 78, 245, 864, 1091
Hsp92I GRCGYC 1 cut(s) 864
Hsp92II CATG 9 cut(s) 124, 571, 757, 1003, 1018, 1048, 1087, 1169, 1174
HspAI GCGC 1 cut(s) 268
Kzo9I GATC 2 cut(s) 466, 914
LguI GCTCTTC 1 cut(s) 615
LmnI GCTCC 2 cut(s) 593, 1153
Lsp1109I GCAGC 2 cut(s) 1082, 1229
MaeI CTAG 3 cut(s) 647, 695, 1058
MaeII ACGT 4 cut(s) 78, 245, 864, 1091
MaeIII GTNAC 2 cut(s) 178, 679
MalI GATC 2 cut(s) 468, 916
MboI GATC 2 cut(s) 466, 914
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 987
MluCI AATT 5 cut(s) 59, 239, 507, 784, 1030
MlyI GAGTC 1 cut(s) 268
MseI TTAA 2 cut(s) 92, 242
MspI CCGG 3 cut(s) 271, 564, 876
MspR9I CCNGG 5 cut(s) 283, 564, 742, 921, 1149
MvaI CCWGG 4 cut(s) 283, 742, 921, 1149
MwoI GCNNNNNNNGC 7 cut(s) 23, 51, 218, 479, 1067, 1144, 1153
NciI CCSGG 1 cut(s) 564
NcoI CCATGG 3 cut(s) 753, 1014, 1170
NdeII GATC 2 cut(s) 466, 914
NheI GCTAGC 1 cut(s) 1057
NlaIII CATG 9 cut(s) 124, 571, 757, 1003, 1018, 1048, 1087, 1169, 1174
NlaIV GGNNCC 2 cut(s) 561, 1183
NmuCI GTSAC 1 cut(s) 679
NspI RCATGY 4 cut(s) 124, 1003, 1048, 1169
NspV TTCGAA 1 cut(s) 414
PaqCI CACCTGC 1 cut(s) 970
PciI ACATGT 2 cut(s) 120, 1044
PciSI GCTCTTC 1 cut(s) 615
PfeI GAWTC 1 cut(s) 1120
PflFI GACNNNGTC 1 cut(s) 1105
PkrI GCNGC 2 cut(s) 1072, 1219
PleI GAGTC 1 cut(s) 267
PpsI GAGTC 1 cut(s) 267
Ppu21I YACGTR 1 cut(s) 79
PpuMI RGGWCCY 1 cut(s) 559
PscI ACATGT 2 cut(s) 120, 1044
PsiI TTATAA 1 cut(s) 254
Psp5II RGGWCCY 1 cut(s) 559
Psp6I CCWGG 4 cut(s) 281, 740, 919, 1147
PspGI CCWGG 4 cut(s) 281, 740, 919, 1147
PspN4I GGNNCC 2 cut(s) 561, 1183
PspPI GGNCC 2 cut(s) 559, 1182
PspPPI RGGWCCY 1 cut(s) 559
PsrI GAACNNNNNNTAC 2 cut(s) 956, 988
PsuI RGATCY 1 cut(s) 466
PsyI GACNNNGTC 1 cut(s) 1105
RsaI GTAC 2 cut(s) 403, 1088
RsaNI GTAC 2 cut(s) 402, 1087
SalI GTCGAC 1 cut(s) 124
SapI GCTCTTC 1 cut(s) 615
SaqAI TTAA 2 cut(s) 92, 242
SatI GCNGC 2 cut(s) 1071, 1218
Sau3AI GATC 2 cut(s) 466, 914
Sau96I GGNCC 2 cut(s) 559, 1182
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 1 cut(s) 268
ScrFI CCNGG 5 cut(s) 283, 564, 742, 921, 1149
SduI GDGCHC 1 cut(s) 987
SfcI CTRYAG 2 cut(s) 130, 552
SfuI TTCGAA 1 cut(s) 414
SinI GGWCC 2 cut(s) 559, 1182
Sse9I AATT 5 cut(s) 59, 239, 507, 784, 1030
SsiI CCGC 2 cut(s) 210, 343
SspMI CTAG 3 cut(s) 647, 695, 1058
StyD4I CCNGG 5 cut(s) 281, 562, 740, 919, 1147
StyI CCWWGG 5 cut(s) 753, 837, 1014, 1170, 1185
TaaI ACNGT 7 cut(s) 155, 216, 227, 325, 643, 701, 1107
TaiI ACGT 4 cut(s) 81, 248, 867, 1094
TaqI TCGA 7 cut(s) 125, 248, 396, 414, 1050, 1096, 1112
TasI AATT 5 cut(s) 59, 239, 507, 784, 1030
TatI WGTACW 2 cut(s) 401, 1086
TfiI GAWTC 1 cut(s) 1120
Tru1I TTAA 2 cut(s) 92, 242
Tru9I TTAA 2 cut(s) 92, 242
TscAI CASTG 4 cut(s) 646, 706, 1110, 1137
TseFI GTSAC 1 cut(s) 679
TseI GCWGC 2 cut(s) 1070, 1217
Tsp45I GTSAC 1 cut(s) 679
TspDTI ATGAA 3 cut(s) 393, 720, 1052
TspGWI ACGGA 2 cut(s) 698, 744
TspRI CASTG 4 cut(s) 646, 706, 1110, 1137
Tth111I GACNNNGTC 1 cut(s) 1105
VneI GTGCAC 1 cut(s) 983
VpaK11BI GGWCC 2 cut(s) 559, 1182
XapI RAATTY 1 cut(s) 784
XbaI TCTAGA 1 cut(s) 646
XceI RCATGY 4 cut(s) 124, 1003, 1048, 1169
XmiI GTMKAC 1 cut(s) 125
XspI CTAG 3 cut(s) 647, 695, 1058
ZraI GACGTC 1 cut(s) 865
ZrmI AGTACT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.