Rh5BG381600

Belongs to the histone H2B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0017481
Physical Location & Seq
Forward (+)
60545907 .. 60547627
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG381600.1

Sequence Viewer

Length: 1167 bp
ATGTCAACCAGTAGTAAACCCCCCAACACGTACAGTTATTATCTCCTCAATCCTACTGTAACTTCAGTCTTTATCACAAACAATATCATCGCACCGTTTTCAAATCCATGTTCTGAGAGGGAGAGAGCTAACGACTCGGTTTTGTTTAGTAAGAAAACATCGATCATAGGTCCATTACTGTCGAAAATGGCTCCGAAGCGCCGCTCATCATCCAGGTTGATGATGAAGATCACCAAGCAAGTTGTCAAGGAAACTGTTGAAGTCTCTGTGGTCCAAAGTAAGAAGAGGCAGAAGAAAGTGAATGATGATGAACTACAAGAGTCACCCAATAGTACAATTGACATCGAAACCAAAGAAGAAAACCAGACCGAGAAAATTGAAGTCTTGGTTGACCAAAAGCCAGCCAGGATTGTTGCCATTGAAAGCCAAGAAGAAAGTGAAACCCCAACTGGTGAACAAGACAAAGGGCCAGTCAAGGCTGTTGCACAAGAACAAGAAGGTGCGACTCCAAATGTTGAAGAGACACAGCCAGAGGAAAAAGGCACAGATCCAATTGACAAAGAGCCAGTCAGCAAGGATGTTGCCATAATGGAAACTGAACAAGAACAAGAAGAAAGTGAAACGCAAAACTTTGAAGAAGATAGAGAGCACGCCAAGACTATTTCCATGGATATCCACGAAGAAAGTGGAGCACAAAATTTTGAAGACAAAAAACCAAAGGAGACCCCTATGACTCCTGAGAAGAAAGAAGACAACCAAGCATCCAAGGGTGAAGAGGAGAAGAGTGATGATGTCAAGCAGATCGGAGAAGGAGGGGAGAAGAAAGAAAAGAAGAGAAAGAGGAGATCAACAGGGAAGAATAGAGAGGGAGGAGAGGGGTATAAGAGGTATGTGTATAAAGTGTTGAAGCAGGTGCACCCTGAACTGGGGATGTCAGCAAAGGCCATGGTGGTTCTCAACAATTATATGAATGACATGTTCGAGAGGCTAGCTGATGAGGCAGCAAAGCTGGCCATGTACACGGCGCGAAAGACGCTGTCTTCGAGGGAGATTCAAGGGGCAGTGAAGCTGGTTTTGCCTGGAGAGCTTGGGAAACATGCCATGGCGGAAGGGACCAAGGCTGTGAGCAACTACTTGTCCAAGAATGCAGCAAGGTCCAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

388

Amino Acids

43.51

Weight (kDa)

5.67

Isoelectric Point (pI)

55.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 275 - 357 3.4e-18 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08170
fragaria_vesca FvH4_3g30460
malus_domestica MD11G1150000.v1.1
prunus_persica Prupe.6G114300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0056411
rosa_laevigata RLG00000035096
rosa_multiflora Rmu_sc0000317.1_g000038
rosa_rugosa Rorug05G0299300
rosa_samantha Rh5AG370200 Rh5BG381400 Rh5BG381600
rosa_wichuraiana Rw5G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 901
Acc36I ACCTGC 1 cut(s) 901
AccBSI CCGCTC 1 cut(s) 204
AccII CGCG 1 cut(s) 1027
AciI CCGC 2 cut(s) 202, 1106
AclWI GGATC 1 cut(s) 542
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 32, 334, 1019
AfiI CCNNNNNNNGG 2 cut(s) 925, 926
AflIII ACRYGT 2 cut(s) 27, 975
AgsI TTSAA 9 cut(s) 102, 260, 380, 422, 518, 635, 704, 907, 1055
AjnI CCWGG 3 cut(s) 212, 404, 1078
AluBI AGCT 5 cut(s) 128, 992, 1009, 1069, 1087
AluI AGCT 5 cut(s) 128, 992, 1009, 1069, 1087
Alw21I GWGCWC 3 cut(s) 651, 694, 918
Alw26I GTCTC 3 cut(s) 268, 515, 716
Alw44I GTGCAC 1 cut(s) 914
AlwI GGATC 1 cut(s) 542
AoxI GGCC 3 cut(s) 467, 942, 1011
ApaLI GTGCAC 1 cut(s) 914
ApeKI GCWGC 2 cut(s) 1001, 1148
ApoI RAATTY 1 cut(s) 697
ArsI GACNNNNNNTTYG 6 cut(s) 456, 488, 552, 584, 1024, 1056
AspLEI GCGC 2 cut(s) 201, 1027
AspS9I GGNCC 5 cut(s) 170, 271, 467, 1113, 1155
AsuHPI GGTGA 4 cut(s) 223, 315, 464, 782
AsuNHI GCTAGC 1 cut(s) 988
AvaII GGWCC 4 cut(s) 170, 271, 1113, 1155
BaeGI GKGCMC 1 cut(s) 918
BalI TGGCCA 1 cut(s) 1013
BbsI GAAGAC 3 cut(s) 711, 756, 1032
Bbv12I GWGCWC 3 cut(s) 651, 694, 918
BbvI GCAGC 2 cut(s) 1013, 1160
BceAI ACGGC 1 cut(s) 1038
BciT130I CCWGG 3 cut(s) 214, 406, 1080
BcoDI GTCTC 3 cut(s) 268, 515, 716
BfaI CTAG 1 cut(s) 989
BfoI RGCGCY 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 3 cut(s) 202, 1002, 1149
BlsI GCNGC 3 cut(s) 203, 1003, 1150
Bme1390I CCNGG 3 cut(s) 214, 406, 1080
Bme18I GGWCC 4 cut(s) 170, 271, 1113, 1155
BmgT120I GGNCC 5 cut(s) 170, 271, 467, 1113, 1155
BmiI GGNNCC 2 cut(s) 192, 1114
BmrFI CCNGG 3 cut(s) 214, 406, 1080
BmrI ACTGGG 1 cut(s) 935
BmsI GCATC 1 cut(s) 770
BmtI GCTAGC 1 cut(s) 992
BmuI ACTGGG 1 cut(s) 935
BpiI GAAGAC 3 cut(s) 711, 756, 1032
BpmI CTGGAG 1 cut(s) 1101
Bsa29I ATCGAT 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 30
BsaBI GATNNNNATC 1 cut(s) 227
BsaI GGTCTC 1 cut(s) 716
BsaJI CCNNGG 5 cut(s) 666, 765, 945, 1101, 1116
Bsc4I CCNNNNNNNGG 2 cut(s) 925, 926
Bse1I ACTGG 5 cut(s) 9, 454, 470, 566, 930
Bse8I GATNNNNATC 1 cut(s) 227
BseBI CCWGG 3 cut(s) 214, 406, 1080
BseCI ATCGAT 1 cut(s) 161
BseDI CCNNGG 5 cut(s) 666, 765, 945, 1101, 1116
BseGI GGATG 4 cut(s) 209, 583, 761, 936
BseJI GATNNNNATC 1 cut(s) 227
BseLI CCNNNNNNNGG 2 cut(s) 925, 926
BseMII CTCAG 2 cut(s) 105, 729
BseNI ACTGG 5 cut(s) 9, 454, 470, 566, 930
BseRI GAGGAG 4 cut(s) 35, 791, 856, 885
BseSI GKGCMC 1 cut(s) 918
BseXI GCAGC 2 cut(s) 1013, 1160
Bsh1236I CGCG 1 cut(s) 1027
BshFI GGCC 3 cut(s) 469, 944, 1013
BshVI ATCGAT 1 cut(s) 161
BsiHKAI GWGCWC 3 cut(s) 651, 694, 918
BslFI GGGAC 1 cut(s) 1126
BslI CCNNNNNNNGG 2 cut(s) 925, 926
BsmAI GTCTC 3 cut(s) 268, 515, 716
BsmFI GGGAC 1 cut(s) 1126
BsmI GAATGC 1 cut(s) 1150
BsnI GGCC 3 cut(s) 469, 944, 1013
Bso31I GGTCTC 1 cut(s) 716
Bsp1286I GDGCHC 3 cut(s) 651, 694, 918
Bsp1407I TGTACA 1 cut(s) 1017
Bsp143I GATC 5 cut(s) 162, 228, 547, 801, 845
Bsp19I CCATGG 3 cut(s) 666, 945, 1101
BspACI CCGC 2 cut(s) 202, 1106
BspANI GGCC 3 cut(s) 469, 944, 1013
BspCNI CTCAG 2 cut(s) 106, 730
BspDI ATCGAT 1 cut(s) 161
BspFNI CGCG 1 cut(s) 1027
BspLI GGNNCC 2 cut(s) 192, 1114
BspMI ACCTGC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 992
BspPI GGATC 1 cut(s) 542
BspTNI GGTCTC 1 cut(s) 716
BsrBI CCGCTC 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 1017
BsrI ACTGG 5 cut(s) 9, 454, 470, 566, 930
BssECI CCNNGG 5 cut(s) 666, 765, 945, 1101, 1116
BssMI GATC 5 cut(s) 162, 228, 547, 801, 845
BssT1I CCWWGG 5 cut(s) 666, 765, 945, 1101, 1116
Bst2UI CCWGG 3 cut(s) 214, 406, 1080
Bst4CI ACNGT 5 cut(s) 35, 58, 96, 180, 256
Bst6I CTCTTC 5 cut(s) 278, 513, 768, 776, 827
BstAUI TGTACA 1 cut(s) 1017
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 4 cut(s) 402, 651, 990, 1011
BstDEI CTNAG 2 cut(s) 114, 738
BstDSI CCRYGG 3 cut(s) 666, 945, 1101
BstF5I GGATG 4 cut(s) 209, 583, 761, 936
BstFNI CGCG 1 cut(s) 1027
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 2 cut(s) 201, 1027
BstKTI GATC 5 cut(s) 165, 231, 550, 804, 848
BstMAI GTCTC 3 cut(s) 268, 515, 716
BstMBI GATC 5 cut(s) 162, 228, 547, 801, 845
BstMWI GCNNNNNNNGC 5 cut(s) 998, 1010, 1033, 1075, 1084
BstNI CCWGG 3 cut(s) 214, 406, 1080
BstNSI RCATGY 2 cut(s) 979, 1100
BstSCI CCNGG 3 cut(s) 212, 404, 1078
BstSLI GKGCMC 1 cut(s) 918
BstUI CGCG 1 cut(s) 1027
BstV1I GCAGC 2 cut(s) 1013, 1160
BstV2I GAAGAC 3 cut(s) 711, 756, 1032
BstX2I RGATCY 1 cut(s) 547
BstYI RGATCY 1 cut(s) 547
Bsu15I ATCGAT 1 cut(s) 161
BsuRI GGCC 3 cut(s) 469, 944, 1013
BsuTUI ATCGAT 1 cut(s) 161
BtgI CCRYGG 3 cut(s) 666, 945, 1101
BtgZI GCGATG 1 cut(s) 73
BtsCI GGATG 4 cut(s) 209, 583, 761, 936
BtsI GCAGTG 1 cut(s) 1068
BtsIMutI CAGTG 1 cut(s) 1068
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 4 cut(s) 402, 651, 990, 1011
CfoI GCGC 2 cut(s) 201, 1027
Cfr13I GGNCC 5 cut(s) 170, 271, 467, 1113, 1155
ClaI ATCGAT 1 cut(s) 161
CseI GACGC 1 cut(s) 1042
Csp6I GTAC 3 cut(s) 31, 333, 1018
CviAII CATG 7 cut(s) 108, 667, 946, 976, 1015, 1097, 1102
CviQI GTAC 3 cut(s) 31, 333, 1018
DdeI CTNAG 2 cut(s) 114, 738
DpnI GATC 5 cut(s) 164, 230, 549, 803, 847
DpnII GATC 5 cut(s) 162, 228, 547, 801, 845
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 5 cut(s) 278, 513, 768, 776, 827
EarI CTCTTC 5 cut(s) 278, 513, 768, 776, 827
EciI GGCGGA 1 cut(s) 1121
Eco130I CCWWGG 5 cut(s) 666, 765, 945, 1101, 1116
Eco31I GGTCTC 1 cut(s) 716
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 4 cut(s) 170, 271, 1113, 1155
Eco57I CTGAAG 1 cut(s) 48
EcoRII CCWGG 3 cut(s) 212, 404, 1078
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 5 cut(s) 666, 765, 945, 1101, 1116
ErhI CCWWGG 5 cut(s) 666, 765, 945, 1101, 1116
FaeI CATG 7 cut(s) 111, 670, 949, 979, 1018, 1100, 1105
FaqI GGGAC 1 cut(s) 1126
FatI CATG 7 cut(s) 107, 666, 945, 975, 1014, 1096, 1101
Fnu4HI GCNGC 3 cut(s) 202, 1002, 1149
FokI GGATG 4 cut(s) 196, 590, 748, 943
Fsp4HI GCNGC 3 cut(s) 202, 1002, 1149
FspBI CTAG 1 cut(s) 989
GlaI GCGC 2 cut(s) 200, 1026
GluI GCNGC 3 cut(s) 202, 1002, 1149
GsuI CTGGAG 1 cut(s) 1101
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 3 cut(s) 469, 944, 1013
HgaI GACGC 1 cut(s) 1042
HhaI GCGC 2 cut(s) 201, 1027
Hin1II CATG 7 cut(s) 111, 670, 949, 979, 1018, 1100, 1105
Hin6I GCGC 2 cut(s) 199, 1025
HinP1I GCGC 2 cut(s) 199, 1025
HincII GTYRAC 2 cut(s) 6, 391
HindII GTYRAC 2 cut(s) 6, 391
HinfI GANTC 5 cut(s) 134, 320, 505, 733, 1051
HphI GGTGA 4 cut(s) 223, 315, 464, 782
Hpy166II GTNNAC 6 cut(s) 6, 17, 391, 455, 916, 1020
Hpy188I TCNGA 3 cut(s) 115, 195, 806
Hpy188III TCNNGA 2 cut(s) 737, 982
Hpy8I GTNNAC 6 cut(s) 6, 17, 391, 455, 916, 1020
HpyAV CCTTC 3 cut(s) 491, 803, 1103
HpyCH4III ACNGT 5 cut(s) 35, 58, 96, 180, 256
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 3 cut(s) 485, 916, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 998, 1010, 1033, 1075, 1084
HpyF3I CTNAG 2 cut(s) 114, 738
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 7 cut(s) 111, 670, 949, 979, 1018, 1100, 1105
HspAI GCGC 2 cut(s) 199, 1025
Kzo9I GATC 5 cut(s) 162, 228, 547, 801, 845
LmnI GCTCC 2 cut(s) 196, 689
Lsp1109I GCAGC 2 cut(s) 1013, 1160
LweI GCATC 1 cut(s) 770
MaeI CTAG 1 cut(s) 989
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 58, 321
MalI GATC 5 cut(s) 164, 230, 549, 803, 847
MbiI CCGCTC 1 cut(s) 204
MboI GATC 5 cut(s) 162, 228, 547, 801, 845
MfeI CAATTG 2 cut(s) 336, 552
MflI RGATCY 1 cut(s) 547
MhlI GDGCHC 3 cut(s) 651, 694, 918
MlsI TGGCCA 1 cut(s) 1013
MluCI AATT 5 cut(s) 336, 375, 552, 697, 961
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 4 cut(s) 128, 329, 499, 727
Mox20I TGGCCA 1 cut(s) 1013
MscI TGGCCA 1 cut(s) 1013
Msp20I TGGCCA 1 cut(s) 1013
MspR9I CCNGG 3 cut(s) 214, 406, 1080
MunI CAATTG 2 cut(s) 336, 552
Mva1269I GAATGC 1 cut(s) 1150
MvaI CCWGG 3 cut(s) 214, 406, 1080
MvnI CGCG 1 cut(s) 1027
MwoI GCNNNNNNNGC 5 cut(s) 998, 1010, 1033, 1075, 1084
NcoI CCATGG 3 cut(s) 666, 945, 1101
NdeII GATC 5 cut(s) 162, 228, 547, 801, 845
NheI GCTAGC 1 cut(s) 988
NlaIII CATG 7 cut(s) 111, 670, 949, 979, 1018, 1100, 1105
NlaIV GGNNCC 2 cut(s) 192, 1114
NmuCI GTSAC 1 cut(s) 321
NspI RCATGY 2 cut(s) 979, 1100
PaqCI CACCTGC 1 cut(s) 901
PciI ACATGT 1 cut(s) 975
PcsI WCGNNNNNNNCGW 1 cut(s) 1040
PctI GAATGC 1 cut(s) 1150
PfeI GAWTC 1 cut(s) 1051
PflFI GACNNNGTC 1 cut(s) 1036
PkrI GCNGC 3 cut(s) 203, 1003, 1150
PleI GAGTC 4 cut(s) 128, 328, 499, 727
PpsI GAGTC 4 cut(s) 128, 328, 499, 727
Ppu21I YACGTR 1 cut(s) 30
PscI ACATGT 1 cut(s) 975
Psp6I CCWGG 3 cut(s) 212, 404, 1078
PspGI CCWGG 3 cut(s) 212, 404, 1078
PspN4I GGNNCC 2 cut(s) 192, 1114
PspPI GGNCC 5 cut(s) 170, 271, 467, 1113, 1155
PsuI RGATCY 1 cut(s) 547
PsyI GACNNNGTC 1 cut(s) 1036
RsaI GTAC 3 cut(s) 32, 334, 1019
RsaNI GTAC 3 cut(s) 31, 333, 1018
SatI GCNGC 3 cut(s) 202, 1002, 1149
Sau3AI GATC 5 cut(s) 162, 228, 547, 801, 845
Sau96I GGNCC 5 cut(s) 170, 271, 467, 1113, 1155
SchI GAGTC 4 cut(s) 128, 329, 499, 727
ScrFI CCNGG 3 cut(s) 214, 406, 1080
SduI GDGCHC 3 cut(s) 651, 694, 918
SfaNI GCATC 1 cut(s) 770
SinI GGWCC 4 cut(s) 170, 271, 1113, 1155
Sse9I AATT 5 cut(s) 336, 375, 552, 697, 961
SsiI CCGC 2 cut(s) 202, 1106
SspMI CTAG 1 cut(s) 989
StyD4I CCNGG 3 cut(s) 212, 404, 1078
StyI CCWWGG 5 cut(s) 666, 765, 945, 1101, 1116
TaaI ACNGT 5 cut(s) 35, 58, 96, 180, 256
TaiI ACGT 1 cut(s) 32
TaqI TCGA 5 cut(s) 161, 182, 345, 981, 1043
TaqII GACCGA 1 cut(s) 383
TasI AATT 5 cut(s) 336, 375, 552, 697, 961
TatI WGTACW 2 cut(s) 332, 1017
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 1 cut(s) 1051
TscAI CASTG 1 cut(s) 1068
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 1001, 1148
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 3 cut(s) 239, 324, 983
TspRI CASTG 1 cut(s) 1068
Tth111I GACNNNGTC 1 cut(s) 1036
VneI GTGCAC 1 cut(s) 914
VpaK11BI GGWCC 4 cut(s) 170, 271, 1113, 1155
XapI RAATTY 1 cut(s) 697
XceI RCATGY 2 cut(s) 979, 1100
XcmI CCANNNNNNNNNTGG 1 cut(s) 683
XspI CTAG 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.