RLG00000035096

Belongs to the histone H2B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0017481
Physical Location & Seq
Forward (+)
60818554 .. 60819543
990 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035096

Sequence Viewer

Length: 990 bp
ATGGCTCCGAAGCGCCGCTCATCATCCAGGTTGGTGGTGAAGACCACCAAGCAAGTTGTCAAGGAAACTGTTGAAGTCTCTGTGGTCCAAAGTAAGAAGAGGCAGAAGAAAGTGAATGATGATGAACTACAAGAGTCGCCCAATAGTACAATTGACATCGAAACCAAAGAAGAAAACCAGACCGAGAAAATTAAAGTCTCGGTTGACAAAAAGCCAGCCAGGATTGTTGCCATTGAAAGCCAAGAAGAAAGTGAAACCCCAACTGGTGAACAAGACAAAGGGCCAGTCAAGGTTGTTGCCATAGAAACCCCACAAGAACAAGAAGGTGCGACTTCAAATGTTGAAGAGACACAGCCAGAGAAAAAAAGCACAGATTTAATTGACAAAGAGCCAGTCAACAAGGACGTTGCCATGGAAACTGAACAAGAACAAGAAGGAAGTGAAACGCAAAACTTTGAAGAAGATAGAGAGCAGGCCAAGACTATTTCCATGGATATCCACGAAGAAAGTGGAACACAAAATTTTGAAGACAAAGAACCAAAGGAGACCCCTATGACTCCTGAGAAGAACGAAGACAACCAAGCATCCAAGGGTGAAGAGGAGAAGAGTGATGGTGTCAAGCAGACCGGAGAAGGAGGGGAGAAGAAAGAAAAGAAGAGAAACAGGAGATCAACAGGGAAGAATAGAGAGGGAGGAGAGGGGTATAAGAGGTATGTGTATAAAGTGTTGAAGCAGGTGCACCCTGAACTGGGGATGTCGGCAAAGGCCATGGTGGTTCTCAACAATTATATGAATGACATGTTCGAGAGGCTAGCTGATGAGGCAGCAAAGCTGGCCATGTACACGGCGCGAAAGACTCTGTCTTCGAGGGAGATTCAAGGGGCAGTGAAGCTGGTTTTGCCTGGAGAGCTTGGGAAACATGCCATGGCAGAAGGGACCAAGGCTGTGAGCAACTACTTGTCCAAGAATGCAGCAAGGTCCAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

330

Amino Acids

36.85

Weight (kDa)

5.48

Isoelectric Point (pI)

56.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 217 - 298 5.5e-18 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 240 - 320 8.2e-06 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08170
fragaria_vesca FvH4_3g30460
malus_domestica MD11G1150000.v1.1
prunus_persica Prupe.6G114300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0056411
rosa_laevigata RLG00000035096
rosa_multiflora Rmu_sc0000317.1_g000038
rosa_rugosa Rorug05G0299300
rosa_samantha Rh5AG370200 Rh5BG381400 Rh5BG381600
rosa_wichuraiana Rw5G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 724
Acc36I ACCTGC 1 cut(s) 724
AccBSI CCGCTC 1 cut(s) 18
AccII CGCG 1 cut(s) 850
AciI CCGC 1 cut(s) 16
AcoI YGGCCR 1 cut(s) 834
AcsI RAATTY 1 cut(s) 520
AfaI GTAC 2 cut(s) 148, 842
AfiI CCNNNNNNNGG 2 cut(s) 748, 749
AflIII ACRYGT 1 cut(s) 798
AgsI TTSAA 8 cut(s) 74, 236, 336, 344, 458, 527, 730, 878
AjnI CCWGG 3 cut(s) 26, 218, 901
AluBI AGCT 4 cut(s) 815, 832, 892, 910
AluI AGCT 4 cut(s) 815, 832, 892, 910
Alw21I GWGCWC 1 cut(s) 741
Alw26I GTCTC 4 cut(s) 82, 202, 341, 539
Alw44I GTGCAC 1 cut(s) 737
AoxI GGCC 4 cut(s) 281, 474, 765, 834
ApaLI GTGCAC 1 cut(s) 737
ApeKI GCWGC 2 cut(s) 824, 971
ApoI RAATTY 1 cut(s) 520
ArsI GACNNNNNNTTYG 6 cut(s) 270, 302, 378, 410, 847, 879
AspLEI GCGC 2 cut(s) 15, 850
AspS9I GGNCC 4 cut(s) 85, 281, 936, 978
AsuHPI GGTGA 3 cut(s) 49, 278, 605
AsuNHI GCTAGC 1 cut(s) 811
AvaII GGWCC 3 cut(s) 85, 936, 978
BaeGI GKGCMC 1 cut(s) 741
BalI TGGCCA 1 cut(s) 836
BbsI GAAGAC 4 cut(s) 47, 534, 579, 855
Bbv12I GWGCWC 1 cut(s) 741
BbvI GCAGC 2 cut(s) 836, 983
BccI CCATC 1 cut(s) 605
BceAI ACGGC 1 cut(s) 861
BciT130I CCWGG 3 cut(s) 28, 220, 903
BcoDI GTCTC 4 cut(s) 82, 202, 341, 539
BfaI CTAG 1 cut(s) 812
BfoI RGCGCY 1 cut(s) 16
BfuAI ACCTGC 1 cut(s) 724
BisI GCNGC 3 cut(s) 16, 825, 972
BlsI GCNGC 3 cut(s) 17, 826, 973
Bme1390I CCNGG 3 cut(s) 28, 220, 903
Bme18I GGWCC 3 cut(s) 85, 936, 978
BmgT120I GGNCC 4 cut(s) 85, 281, 936, 978
BmiI GGNNCC 2 cut(s) 6, 937
BmrFI CCNGG 3 cut(s) 28, 220, 903
BmrI ACTGGG 1 cut(s) 758
BmsI GCATC 1 cut(s) 593
BmtI GCTAGC 1 cut(s) 815
BmuI ACTGGG 1 cut(s) 758
BpiI GAAGAC 4 cut(s) 47, 534, 579, 855
BpmI CTGGAG 1 cut(s) 924
BsaI GGTCTC 1 cut(s) 539
BsaJI CCNNGG 6 cut(s) 411, 489, 588, 768, 924, 939
BsaWI WCCGGW 1 cut(s) 626
Bsc4I CCNNNNNNNGG 2 cut(s) 748, 749
Bse1I ACTGG 4 cut(s) 268, 284, 392, 753
BseBI CCWGG 3 cut(s) 28, 220, 903
BseDI CCNNGG 6 cut(s) 411, 489, 588, 768, 924, 939
BseGI GGATG 3 cut(s) 23, 584, 759
BseLI CCNNNNNNNGG 2 cut(s) 748, 749
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 4 cut(s) 268, 284, 392, 753
BseRI GAGGAG 2 cut(s) 614, 708
BseSI GKGCMC 1 cut(s) 741
BseXI GCAGC 2 cut(s) 836, 983
Bsh1236I CGCG 1 cut(s) 850
BshFI GGCC 4 cut(s) 283, 476, 767, 836
BsiHKAI GWGCWC 1 cut(s) 741
BsiSI CCGG 1 cut(s) 627
BslFI GGGAC 1 cut(s) 949
BslI CCNNNNNNNGG 2 cut(s) 748, 749
BsmAI GTCTC 4 cut(s) 82, 202, 341, 539
BsmFI GGGAC 1 cut(s) 949
BsmI GAATGC 1 cut(s) 973
BsnI GGCC 4 cut(s) 283, 476, 767, 836
Bso31I GGTCTC 1 cut(s) 539
Bsp1286I GDGCHC 1 cut(s) 741
Bsp1407I TGTACA 1 cut(s) 840
Bsp143I GATC 1 cut(s) 668
Bsp19I CCATGG 4 cut(s) 411, 489, 768, 924
BspACI CCGC 1 cut(s) 16
BspANI GGCC 4 cut(s) 283, 476, 767, 836
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 1 cut(s) 850
BspLI GGNNCC 2 cut(s) 6, 937
BspMI ACCTGC 1 cut(s) 724
BspOI GCTAGC 1 cut(s) 815
BspTNI GGTCTC 1 cut(s) 539
BsrBI CCGCTC 1 cut(s) 18
BsrGI TGTACA 1 cut(s) 840
BsrI ACTGG 4 cut(s) 268, 284, 392, 753
BssECI CCNNGG 6 cut(s) 411, 489, 588, 768, 924, 939
BssMI GATC 1 cut(s) 668
BssT1I CCWWGG 6 cut(s) 411, 489, 588, 768, 924, 939
Bst2UI CCWGG 3 cut(s) 28, 220, 903
Bst4CI ACNGT 1 cut(s) 70
Bst6I CTCTTC 5 cut(s) 92, 339, 591, 599, 650
BstAUI TGTACA 1 cut(s) 840
BstC8I GCNNGC 4 cut(s) 216, 474, 813, 834
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 4 cut(s) 411, 489, 768, 924
BstF5I GGATG 3 cut(s) 23, 584, 759
BstFNI CGCG 1 cut(s) 850
BstH2I RGCGCY 1 cut(s) 16
BstHHI GCGC 2 cut(s) 15, 850
BstKTI GATC 1 cut(s) 671
BstMAI GTCTC 4 cut(s) 82, 202, 341, 539
BstMBI GATC 1 cut(s) 668
BstMWI GCNNNNNNNGC 4 cut(s) 821, 833, 898, 907
BstNI CCWGG 3 cut(s) 28, 220, 903
BstNSI RCATGY 2 cut(s) 802, 923
BstSCI CCNGG 3 cut(s) 26, 218, 901
BstSLI GKGCMC 1 cut(s) 741
BstUI CGCG 1 cut(s) 850
BstV1I GCAGC 2 cut(s) 836, 983
BstV2I GAAGAC 4 cut(s) 47, 534, 579, 855
BstXI CCANNNNNNTGG 1 cut(s) 34
BsuRI GGCC 4 cut(s) 283, 476, 767, 836
BtgI CCRYGG 4 cut(s) 411, 489, 768, 924
BtsCI GGATG 3 cut(s) 23, 584, 759
BtsI GCAGTG 1 cut(s) 891
BtsIMutI CAGTG 1 cut(s) 891
BveI ACCTGC 1 cut(s) 724
Cac8I GCNNGC 4 cut(s) 216, 474, 813, 834
CfoI GCGC 2 cut(s) 15, 850
Cfr13I GGNCC 4 cut(s) 85, 281, 936, 978
Csp6I GTAC 2 cut(s) 147, 841
CviAII CATG 7 cut(s) 412, 490, 769, 799, 838, 920, 925
CviQI GTAC 2 cut(s) 147, 841
DdeI CTNAG 1 cut(s) 561
DpnI GATC 1 cut(s) 670
DpnII GATC 1 cut(s) 668
EaeI YGGCCR 1 cut(s) 834
Eam1104I CTCTTC 5 cut(s) 92, 339, 591, 599, 650
EarI CTCTTC 5 cut(s) 92, 339, 591, 599, 650
Eco130I CCWWGG 6 cut(s) 411, 489, 588, 768, 924, 939
Eco31I GGTCTC 1 cut(s) 539
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 3 cut(s) 85, 936, 978
EcoRII CCWGG 3 cut(s) 26, 218, 901
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 6 cut(s) 411, 489, 588, 768, 924, 939
ErhI CCWWGG 6 cut(s) 411, 489, 588, 768, 924, 939
FaeI CATG 7 cut(s) 415, 493, 772, 802, 841, 923, 928
FaqI GGGAC 1 cut(s) 949
FatI CATG 7 cut(s) 411, 489, 768, 798, 837, 919, 924
Fnu4HI GCNGC 3 cut(s) 16, 825, 972
FokI GGATG 3 cut(s) 10, 571, 766
Fsp4HI GCNGC 3 cut(s) 16, 825, 972
FspBI CTAG 1 cut(s) 812
GlaI GCGC 2 cut(s) 14, 849
GluI GCNGC 3 cut(s) 16, 825, 972
GsuI CTGGAG 1 cut(s) 924
HaeII RGCGCY 1 cut(s) 16
HaeIII GGCC 4 cut(s) 283, 476, 767, 836
HapII CCGG 1 cut(s) 627
HhaI GCGC 2 cut(s) 15, 850
Hin1II CATG 7 cut(s) 415, 493, 772, 802, 841, 923, 928
Hin6I GCGC 2 cut(s) 13, 848
HinP1I GCGC 2 cut(s) 13, 848
HincII GTYRAC 2 cut(s) 205, 397
HindII GTYRAC 2 cut(s) 205, 397
HinfI GANTC 4 cut(s) 134, 556, 856, 874
HpaII CCGG 1 cut(s) 627
HphI GGTGA 3 cut(s) 49, 278, 605
Hpy166II GTNNAC 5 cut(s) 205, 269, 397, 739, 843
Hpy188I TCNGA 1 cut(s) 9
Hpy188III TCNNGA 2 cut(s) 560, 805
Hpy8I GTNNAC 5 cut(s) 205, 269, 397, 739, 843
HpyAV CCTTC 4 cut(s) 317, 428, 626, 926
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 2 cut(s) 739, 971
HpyF10VI GCNNNNNNNGC 4 cut(s) 821, 833, 898, 907
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 7 cut(s) 415, 493, 772, 802, 841, 923, 928
HspAI GCGC 2 cut(s) 13, 848
Kzo9I GATC 1 cut(s) 668
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 2 cut(s) 836, 983
LweI GCATC 1 cut(s) 593
MaeI CTAG 1 cut(s) 812
MaeII ACGT 1 cut(s) 405
MalI GATC 1 cut(s) 670
MbiI CCGCTC 1 cut(s) 18
MboI GATC 1 cut(s) 668
MfeI CAATTG 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 741
MlsI TGGCCA 1 cut(s) 836
MluCI AATT 5 cut(s) 150, 189, 378, 520, 784
MluNI TGGCCA 1 cut(s) 836
MlyI GAGTC 3 cut(s) 143, 550, 850
Mox20I TGGCCA 1 cut(s) 836
MscI TGGCCA 1 cut(s) 836
MseI TTAA 2 cut(s) 192, 377
Msp20I TGGCCA 1 cut(s) 836
MspI CCGG 1 cut(s) 627
MspR9I CCNGG 3 cut(s) 28, 220, 903
MunI CAATTG 1 cut(s) 150
Mva1269I GAATGC 1 cut(s) 973
MvaI CCWGG 3 cut(s) 28, 220, 903
MvnI CGCG 1 cut(s) 850
MwoI GCNNNNNNNGC 4 cut(s) 821, 833, 898, 907
NcoI CCATGG 4 cut(s) 411, 489, 768, 924
NdeII GATC 1 cut(s) 668
NheI GCTAGC 1 cut(s) 811
NlaIII CATG 7 cut(s) 415, 493, 772, 802, 841, 923, 928
NlaIV GGNNCC 2 cut(s) 6, 937
NspI RCATGY 2 cut(s) 802, 923
PaqCI CACCTGC 1 cut(s) 724
PciI ACATGT 1 cut(s) 798
PctI GAATGC 1 cut(s) 973
PfeI GAWTC 1 cut(s) 874
PflFI GACNNNGTC 1 cut(s) 859
PkrI GCNGC 3 cut(s) 17, 826, 973
PleI GAGTC 3 cut(s) 142, 550, 850
PpsI GAGTC 3 cut(s) 142, 550, 850
PscI ACATGT 1 cut(s) 798
Psp6I CCWGG 3 cut(s) 26, 218, 901
PspGI CCWGG 3 cut(s) 26, 218, 901
PspN4I GGNNCC 2 cut(s) 6, 937
PspPI GGNCC 4 cut(s) 85, 281, 936, 978
PsyI GACNNNGTC 1 cut(s) 859
RsaI GTAC 2 cut(s) 148, 842
RsaNI GTAC 2 cut(s) 147, 841
SaqAI TTAA 2 cut(s) 192, 377
SatI GCNGC 3 cut(s) 16, 825, 972
Sau3AI GATC 1 cut(s) 668
Sau96I GGNCC 4 cut(s) 85, 281, 936, 978
SchI GAGTC 3 cut(s) 143, 550, 850
ScrFI CCNGG 3 cut(s) 28, 220, 903
SduI GDGCHC 1 cut(s) 741
SfaNI GCATC 1 cut(s) 593
SinI GGWCC 3 cut(s) 85, 936, 978
Sse9I AATT 5 cut(s) 150, 189, 378, 520, 784
SsiI CCGC 1 cut(s) 16
SspMI CTAG 1 cut(s) 812
StyD4I CCNGG 3 cut(s) 26, 218, 901
StyI CCWWGG 6 cut(s) 411, 489, 588, 768, 924, 939
TaaI ACNGT 1 cut(s) 70
TaiI ACGT 1 cut(s) 408
TaqI TCGA 3 cut(s) 159, 804, 866
TaqII GACCGA 1 cut(s) 197
TasI AATT 5 cut(s) 150, 189, 378, 520, 784
TatI WGTACW 2 cut(s) 146, 840
TauI GCSGC 1 cut(s) 18
TfiI GAWTC 1 cut(s) 874
Tru1I TTAA 2 cut(s) 192, 377
Tru9I TTAA 2 cut(s) 192, 377
TscAI CASTG 1 cut(s) 891
TseI GCWGC 2 cut(s) 824, 971
TspDTI ATGAA 2 cut(s) 138, 806
TspRI CASTG 1 cut(s) 891
Tth111I GACNNNGTC 1 cut(s) 859
VneI GTGCAC 1 cut(s) 737
VpaK11BI GGWCC 3 cut(s) 85, 936, 978
XapI RAATTY 1 cut(s) 520
XceI RCATGY 2 cut(s) 802, 923
XcmI CCANNNNNNNNNTGG 1 cut(s) 506
XspI CTAG 1 cut(s) 812
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.