Rw5G034680

Belongs to the histone H2B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0017481
Physical Location & Seq
Forward (+)
59802879 .. 59804057
1179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G034680.1

Sequence Viewer

Length: 1179 bp
ATGTCAACCAGTAGTAAACCCCCCAACACGTACAGTTATTATCTCCTCAATCCTGCTGTAACTTCAGTCTTTATCACAAACAATATCATCGCACCGTTTTCAAATCCATGTTCTGAGAGGGAGAGAGCTAACGACTCGGTTTTGTTTAGTAAGAAAACATCGATCATAGGTCCATTACTGTCGAAAATGGCTCCGAAGCGCCGCTCATCATCCAGGTTGATGATGAAGACCACCAAGCAAGTTGTCAAGGAAACTGTTGAATTCTCTGTGGTCCAAAGTAAGAAGAGGCAGAAGAAAGTGAATGATGATGAACTACAAGAGTCACCCAATAGTACAATTGACATCGAAACCAAAGAAGAAAACCAGACCGAGAAAATTGAAGTCTTGGTTGACCAAAAGCCAGCCAGGATTGTTGCCATTGAAAGCCAAGAAGAAAGTGAAACCCCAACTGGTGAACAAGACAAAGGGCCAGTCAAGGTTGTTGCCATAGAAACCCCACAAGAACAAGAAGGTGCGACTCCAAATGTTGAAGAGACACAGCCAGAGGAAAAAGGCACAGATCCAATTGACAAAGAGCCAGTCAGCAAGGATGTTGCCATAATGGAAACTGAACAAGAACAAGAAGAAAGTGAAACGCAGAACTTTGAAGAAGATAGAGAGCACGCCAAGACTATTTCCATGGATATCCACGAAGAAAGTGAAACACAAAATTTTGAAGACAAAAAACCAAAGGAGACCCCTATCACTCCTGAGAAGAAAGAAGACAACCAAGCATCCAAGGGTGAAGAGGAGAAGAGTGATGATGTCAAGCAGATCGGAGAAGGAGGGGAGAAGAAAGAAAAGAAGAGAAAGAGGAGATCAACAGGGAAGAATAGAGAGGGAGGAGAGGGGTATAAGAGGTATGTGTATAAAGTGTTGAAGCAGGTGCACCCTGAACTGGGGATGTCAGCAAAGGCCATGGTGGTTCTCAACAATTATATGAATGACATGTTCGAGAGGCTAGCTGATGAGGCAGCAAAGCTGGCCATGTACACGGCGCGAAAGACGCTGTCTTCGAGGGAGATTCAAGGGGCAGTGAAGCTGGTTTTGCCTGGAGAGCTTGGGAAACATGCCATGGCGGAAGGGACCAAGGCTGTGAGCAACTACTTGTCCAAGAATGCAGCAAGGTCCAATAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

392

Amino Acids

44.06

Weight (kDa)

5.5

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 279 - 361 3.5e-18 Core histone H2A/H2B/H3/H4 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017481)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08170
fragaria_vesca FvH4_3g30460
malus_domestica MD11G1150000.v1.1
prunus_persica Prupe.6G114300_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0056411
rosa_laevigata RLG00000035096
rosa_multiflora Rmu_sc0000317.1_g000038
rosa_rugosa Rorug05G0299300
rosa_samantha Rh5AG370200 Rh5BG381400 Rh5BG381600
rosa_wichuraiana Rw5G034680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 913
Acc36I ACCTGC 1 cut(s) 913
AccBSI CCGCTC 1 cut(s) 204
AccII CGCG 1 cut(s) 1039
AciI CCGC 2 cut(s) 202, 1118
AclWI GGATC 1 cut(s) 554
AcoI YGGCCR 1 cut(s) 1023
AcsI RAATTY 2 cut(s) 260, 709
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 3 cut(s) 32, 334, 1031
AfiI CCNNNNNNNGG 2 cut(s) 937, 938
AflIII ACRYGT 2 cut(s) 27, 987
AgsI TTSAA 9 cut(s) 102, 260, 380, 422, 530, 647, 716, 919, 1067
AjnI CCWGG 3 cut(s) 212, 404, 1090
AluBI AGCT 5 cut(s) 128, 1004, 1021, 1081, 1099
AluI AGCT 5 cut(s) 128, 1004, 1021, 1081, 1099
Alw21I GWGCWC 2 cut(s) 663, 930
Alw26I GTCTC 2 cut(s) 527, 728
Alw44I GTGCAC 1 cut(s) 926
AlwI GGATC 1 cut(s) 554
AoxI GGCC 3 cut(s) 467, 954, 1023
ApaLI GTGCAC 1 cut(s) 926
ApeKI GCWGC 2 cut(s) 1013, 1160
ApoI RAATTY 2 cut(s) 260, 709
ArsI GACNNNNNNTTYG 6 cut(s) 456, 488, 564, 596, 1036, 1068
AspLEI GCGC 2 cut(s) 201, 1039
AspS9I GGNCC 5 cut(s) 170, 271, 467, 1125, 1167
AsuHPI GGTGA 3 cut(s) 315, 464, 794
AsuNHI GCTAGC 1 cut(s) 1000
AvaII GGWCC 4 cut(s) 170, 271, 1125, 1167
BaeGI GKGCMC 1 cut(s) 930
BalI TGGCCA 1 cut(s) 1025
BbsI GAAGAC 4 cut(s) 233, 723, 768, 1044
Bbv12I GWGCWC 2 cut(s) 663, 930
BbvI GCAGC 2 cut(s) 1025, 1172
BceAI ACGGC 1 cut(s) 1050
BciT130I CCWGG 3 cut(s) 214, 406, 1092
BcoDI GTCTC 2 cut(s) 527, 728
BfaI CTAG 1 cut(s) 1001
BfoI RGCGCY 1 cut(s) 202
BfuAI ACCTGC 1 cut(s) 913
BisI GCNGC 3 cut(s) 202, 1014, 1161
BlsI GCNGC 3 cut(s) 203, 1015, 1162
Bme1390I CCNGG 3 cut(s) 214, 406, 1092
Bme18I GGWCC 4 cut(s) 170, 271, 1125, 1167
BmgT120I GGNCC 5 cut(s) 170, 271, 467, 1125, 1167
BmiI GGNNCC 2 cut(s) 192, 1126
BmrFI CCNGG 3 cut(s) 214, 406, 1092
BmrI ACTGGG 1 cut(s) 947
BmsI GCATC 1 cut(s) 782
BmtI GCTAGC 1 cut(s) 1004
BmuI ACTGGG 1 cut(s) 947
BpiI GAAGAC 4 cut(s) 233, 723, 768, 1044
BpmI CTGGAG 1 cut(s) 1113
Bsa29I ATCGAT 1 cut(s) 161
BsaAI YACGTR 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 5 cut(s) 678, 777, 957, 1113, 1128
Bsc4I CCNNNNNNNGG 2 cut(s) 937, 938
Bse1I ACTGG 5 cut(s) 9, 454, 470, 578, 942
BseBI CCWGG 3 cut(s) 214, 406, 1092
BseCI ATCGAT 1 cut(s) 161
BseDI CCNNGG 5 cut(s) 678, 777, 957, 1113, 1128
BseGI GGATG 4 cut(s) 209, 595, 773, 948
BseLI CCNNNNNNNGG 2 cut(s) 937, 938
BseMII CTCAG 2 cut(s) 105, 741
BseNI ACTGG 5 cut(s) 9, 454, 470, 578, 942
BseRI GAGGAG 4 cut(s) 35, 803, 868, 897
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 2 cut(s) 1025, 1172
Bsh1236I CGCG 1 cut(s) 1039
BshFI GGCC 3 cut(s) 469, 956, 1025
BshVI ATCGAT 1 cut(s) 161
BsiHKAI GWGCWC 2 cut(s) 663, 930
BslFI GGGAC 1 cut(s) 1138
BslI CCNNNNNNNGG 2 cut(s) 937, 938
BsmAI GTCTC 2 cut(s) 527, 728
BsmFI GGGAC 1 cut(s) 1138
BsmI GAATGC 1 cut(s) 1162
BsnI GGCC 3 cut(s) 469, 956, 1025
Bso31I GGTCTC 1 cut(s) 728
Bsp1286I GDGCHC 2 cut(s) 663, 930
Bsp1407I TGTACA 1 cut(s) 1029
Bsp143I GATC 4 cut(s) 162, 559, 813, 857
Bsp19I CCATGG 3 cut(s) 678, 957, 1113
BspACI CCGC 2 cut(s) 202, 1118
BspANI GGCC 3 cut(s) 469, 956, 1025
BspCNI CTCAG 2 cut(s) 106, 742
BspDI ATCGAT 1 cut(s) 161
BspFNI CGCG 1 cut(s) 1039
BspLI GGNNCC 2 cut(s) 192, 1126
BspMI ACCTGC 1 cut(s) 913
BspOI GCTAGC 1 cut(s) 1004
BspPI GGATC 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 728
BsrBI CCGCTC 1 cut(s) 204
BsrGI TGTACA 1 cut(s) 1029
BsrI ACTGG 5 cut(s) 9, 454, 470, 578, 942
BssECI CCNNGG 5 cut(s) 678, 777, 957, 1113, 1128
BssMI GATC 4 cut(s) 162, 559, 813, 857
BssT1I CCWWGG 5 cut(s) 678, 777, 957, 1113, 1128
Bst2UI CCWGG 3 cut(s) 214, 406, 1092
Bst4CI ACNGT 4 cut(s) 35, 96, 180, 256
Bst6I CTCTTC 5 cut(s) 278, 525, 780, 788, 839
BstAUI TGTACA 1 cut(s) 1029
BstBAI YACGTR 1 cut(s) 30
BstC8I GCNNGC 4 cut(s) 402, 663, 1002, 1023
BstDEI CTNAG 2 cut(s) 114, 750
BstDSI CCRYGG 3 cut(s) 678, 957, 1113
BstF5I GGATG 4 cut(s) 209, 595, 773, 948
BstFNI CGCG 1 cut(s) 1039
BstH2I RGCGCY 1 cut(s) 202
BstHHI GCGC 2 cut(s) 201, 1039
BstKTI GATC 4 cut(s) 165, 562, 816, 860
BstMAI GTCTC 2 cut(s) 527, 728
BstMBI GATC 4 cut(s) 162, 559, 813, 857
BstMWI GCNNNNNNNGC 5 cut(s) 1010, 1022, 1045, 1087, 1096
BstNI CCWGG 3 cut(s) 214, 406, 1092
BstNSI RCATGY 2 cut(s) 991, 1112
BstSCI CCNGG 3 cut(s) 212, 404, 1090
BstSLI GKGCMC 1 cut(s) 930
BstUI CGCG 1 cut(s) 1039
BstV1I GCAGC 2 cut(s) 1025, 1172
BstV2I GAAGAC 4 cut(s) 233, 723, 768, 1044
BstX2I RGATCY 1 cut(s) 559
BstYI RGATCY 1 cut(s) 559
Bsu15I ATCGAT 1 cut(s) 161
BsuRI GGCC 3 cut(s) 469, 956, 1025
BsuTUI ATCGAT 1 cut(s) 161
BtgI CCRYGG 3 cut(s) 678, 957, 1113
BtgZI GCGATG 1 cut(s) 73
BtsCI GGATG 4 cut(s) 209, 595, 773, 948
BtsI GCAGTG 1 cut(s) 1080
BtsIMutI CAGTG 1 cut(s) 1080
BveI ACCTGC 1 cut(s) 913
Cac8I GCNNGC 4 cut(s) 402, 663, 1002, 1023
CfoI GCGC 2 cut(s) 201, 1039
Cfr13I GGNCC 5 cut(s) 170, 271, 467, 1125, 1167
ClaI ATCGAT 1 cut(s) 161
CseI GACGC 1 cut(s) 1054
Csp6I GTAC 3 cut(s) 31, 333, 1030
CviAII CATG 7 cut(s) 108, 679, 958, 988, 1027, 1109, 1114
CviQI GTAC 3 cut(s) 31, 333, 1030
DdeI CTNAG 2 cut(s) 114, 750
DpnI GATC 4 cut(s) 164, 561, 815, 859
DpnII GATC 4 cut(s) 162, 559, 813, 857
EaeI YGGCCR 1 cut(s) 1023
Eam1104I CTCTTC 5 cut(s) 278, 525, 780, 788, 839
EarI CTCTTC 5 cut(s) 278, 525, 780, 788, 839
EciI GGCGGA 1 cut(s) 1133
Eco130I CCWWGG 5 cut(s) 678, 777, 957, 1113, 1128
Eco31I GGTCTC 1 cut(s) 728
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 4 cut(s) 170, 271, 1125, 1167
Eco57I CTGAAG 1 cut(s) 48
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 3 cut(s) 212, 404, 1090
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 5 cut(s) 678, 777, 957, 1113, 1128
ErhI CCWWGG 5 cut(s) 678, 777, 957, 1113, 1128
FaeI CATG 7 cut(s) 111, 682, 961, 991, 1030, 1112, 1117
FaqI GGGAC 1 cut(s) 1138
FatI CATG 7 cut(s) 107, 678, 957, 987, 1026, 1108, 1113
Fnu4HI GCNGC 3 cut(s) 202, 1014, 1161
FokI GGATG 4 cut(s) 196, 602, 760, 955
Fsp4HI GCNGC 3 cut(s) 202, 1014, 1161
FspBI CTAG 1 cut(s) 1001
GlaI GCGC 2 cut(s) 200, 1038
GluI GCNGC 3 cut(s) 202, 1014, 1161
GsuI CTGGAG 1 cut(s) 1113
HaeII RGCGCY 1 cut(s) 202
HaeIII GGCC 3 cut(s) 469, 956, 1025
HgaI GACGC 1 cut(s) 1054
HhaI GCGC 2 cut(s) 201, 1039
Hin1II CATG 7 cut(s) 111, 682, 961, 991, 1030, 1112, 1117
Hin6I GCGC 2 cut(s) 199, 1037
HinP1I GCGC 2 cut(s) 199, 1037
HincII GTYRAC 2 cut(s) 6, 391
HindII GTYRAC 2 cut(s) 6, 391
HinfI GANTC 4 cut(s) 134, 320, 517, 1063
HphI GGTGA 3 cut(s) 315, 464, 794
Hpy166II GTNNAC 6 cut(s) 6, 17, 391, 455, 928, 1032
Hpy188I TCNGA 3 cut(s) 115, 195, 818
Hpy188III TCNNGA 2 cut(s) 749, 994
Hpy8I GTNNAC 6 cut(s) 6, 17, 391, 455, 928, 1032
HpyAV CCTTC 3 cut(s) 503, 815, 1115
HpyCH4III ACNGT 4 cut(s) 35, 96, 180, 256
HpyCH4IV ACGT 1 cut(s) 29
HpyCH4V TGCA 2 cut(s) 928, 1160
HpyF10VI GCNNNNNNNGC 5 cut(s) 1010, 1022, 1045, 1087, 1096
HpyF3I CTNAG 2 cut(s) 114, 750
HpySE526I ACGT 1 cut(s) 29
Hsp92II CATG 7 cut(s) 111, 682, 961, 991, 1030, 1112, 1117
HspAI GCGC 2 cut(s) 199, 1037
Kzo9I GATC 4 cut(s) 162, 559, 813, 857
LmnI GCTCC 1 cut(s) 196
Lsp1109I GCAGC 2 cut(s) 1025, 1172
LweI GCATC 1 cut(s) 782
MaeI CTAG 1 cut(s) 1001
MaeII ACGT 1 cut(s) 29
MaeIII GTNAC 2 cut(s) 58, 321
MalI GATC 4 cut(s) 164, 561, 815, 859
MbiI CCGCTC 1 cut(s) 204
MboI GATC 4 cut(s) 162, 559, 813, 857
MfeI CAATTG 2 cut(s) 336, 564
MflI RGATCY 1 cut(s) 559
MhlI GDGCHC 2 cut(s) 663, 930
MlsI TGGCCA 1 cut(s) 1025
MluCI AATT 6 cut(s) 260, 336, 375, 564, 709, 973
MluNI TGGCCA 1 cut(s) 1025
MlyI GAGTC 3 cut(s) 128, 329, 511
Mox20I TGGCCA 1 cut(s) 1025
MscI TGGCCA 1 cut(s) 1025
Msp20I TGGCCA 1 cut(s) 1025
MspR9I CCNGG 3 cut(s) 214, 406, 1092
MunI CAATTG 2 cut(s) 336, 564
Mva1269I GAATGC 1 cut(s) 1162
MvaI CCWGG 3 cut(s) 214, 406, 1092
MvnI CGCG 1 cut(s) 1039
MwoI GCNNNNNNNGC 5 cut(s) 1010, 1022, 1045, 1087, 1096
NcoI CCATGG 3 cut(s) 678, 957, 1113
NdeII GATC 4 cut(s) 162, 559, 813, 857
NheI GCTAGC 1 cut(s) 1000
NlaIII CATG 7 cut(s) 111, 682, 961, 991, 1030, 1112, 1117
NlaIV GGNNCC 2 cut(s) 192, 1126
NmuCI GTSAC 1 cut(s) 321
NspI RCATGY 2 cut(s) 991, 1112
PaqCI CACCTGC 1 cut(s) 913
PciI ACATGT 1 cut(s) 987
PcsI WCGNNNNNNNCGW 1 cut(s) 1052
PctI GAATGC 1 cut(s) 1162
PfeI GAWTC 1 cut(s) 1063
PflFI GACNNNGTC 1 cut(s) 1048
PkrI GCNGC 3 cut(s) 203, 1015, 1162
PleI GAGTC 3 cut(s) 128, 328, 511
PpsI GAGTC 3 cut(s) 128, 328, 511
Ppu21I YACGTR 1 cut(s) 30
PscI ACATGT 1 cut(s) 987
Psp6I CCWGG 3 cut(s) 212, 404, 1090
PspGI CCWGG 3 cut(s) 212, 404, 1090
PspN4I GGNNCC 2 cut(s) 192, 1126
PspPI GGNCC 5 cut(s) 170, 271, 467, 1125, 1167
PsuI RGATCY 1 cut(s) 559
PsyI GACNNNGTC 1 cut(s) 1048
RsaI GTAC 3 cut(s) 32, 334, 1031
RsaNI GTAC 3 cut(s) 31, 333, 1030
SatI GCNGC 3 cut(s) 202, 1014, 1161
Sau3AI GATC 4 cut(s) 162, 559, 813, 857
Sau96I GGNCC 5 cut(s) 170, 271, 467, 1125, 1167
SchI GAGTC 3 cut(s) 128, 329, 511
ScrFI CCNGG 3 cut(s) 214, 406, 1092
SduI GDGCHC 2 cut(s) 663, 930
SfaNI GCATC 1 cut(s) 782
SinI GGWCC 4 cut(s) 170, 271, 1125, 1167
Sse9I AATT 6 cut(s) 260, 336, 375, 564, 709, 973
SsiI CCGC 2 cut(s) 202, 1118
SspMI CTAG 1 cut(s) 1001
StyD4I CCNGG 3 cut(s) 212, 404, 1090
StyI CCWWGG 5 cut(s) 678, 777, 957, 1113, 1128
TaaI ACNGT 4 cut(s) 35, 96, 180, 256
TaiI ACGT 1 cut(s) 32
TaqI TCGA 5 cut(s) 161, 182, 345, 993, 1055
TaqII GACCGA 1 cut(s) 383
TasI AATT 6 cut(s) 260, 336, 375, 564, 709, 973
TatI WGTACW 2 cut(s) 332, 1029
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 1 cut(s) 1063
TscAI CASTG 1 cut(s) 1080
TseFI GTSAC 1 cut(s) 321
TseI GCWGC 2 cut(s) 1013, 1160
Tsp45I GTSAC 1 cut(s) 321
TspDTI ATGAA 3 cut(s) 239, 324, 995
TspRI CASTG 1 cut(s) 1080
Tth111I GACNNNGTC 1 cut(s) 1048
VneI GTGCAC 1 cut(s) 926
VpaK11BI GGWCC 4 cut(s) 170, 271, 1125, 1167
XapI RAATTY 2 cut(s) 260, 709
XceI RCATGY 2 cut(s) 991, 1112
XspI CTAG 1 cut(s) 1001
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.