FvH4_3g31260

SNF1-related protein kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008047
Physical Location & Seq
Reverse (-)
25139955 .. 25146397
6443 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g31260.t1

Sequence Viewer

Length: 354 bp
ATGAACAACTCATATGATGATGATTATGATGAAGCAACTGTTGCAGGATTTGAAGTTATAAGATCACCTGATACAAGTTACAACAATACGTATCCTGGGAATGAAGATGAAGCACGTGATCCACCCATTGTCCCTCCACACCTTCAACAGACCTTACTTAGTTATCCAGCAAGCGGAGACACTACTGGAACTCTTCCTTTGCCACCAAATGTGACCCTCAACCATCTTTACATTGAGAATCGGGAGAGCCCCCGGTCTGTGGTTGCACTTGGATTCACTCATCGCTTTCGTTCTAAATTTGTTACTGTTGTGCTTTACAAACCAGTTCAAAGAAAGGGGGCTAGCAGCACTTAG

Protein Analysis

118

Amino Acids

13.03

Weight (kDa)

5.2

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPKBI PF04739 38 - 108 8e-25 5'-AMP-activated protein kinase beta subunit, interaction domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 59
AciI CCGC 1 cut(s) 174
AclWI GGATC 1 cut(s) 113
AcsI RAATTY 1 cut(s) 296
AcvI CACGTG 1 cut(s) 116
AfiI CCNNNNNNNGG 2 cut(s) 173, 259
AgsI TTSAA 3 cut(s) 53, 146, 329
AjnI CCWGG 1 cut(s) 94
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 113
ApeKI GCWGC 1 cut(s) 345
ApoI RAATTY 1 cut(s) 296
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 57
AsuNHI GCTAGC 1 cut(s) 341
BanII GRGCYC 1 cut(s) 251
BbrPI CACGTG 1 cut(s) 116
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 96
BciVI GTATCC 1 cut(s) 102
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 342
BfuI GTATCC 1 cut(s) 102
BisI GCNGC 1 cut(s) 346
BlsI GCNGC 1 cut(s) 347
Bme1390I CCNGG 2 cut(s) 96, 253
BmrFI CCNGG 2 cut(s) 96, 253
BmtI GCTAGC 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 253
BsaAI YACGTR 2 cut(s) 90, 116
BsaJI CCNNGG 2 cut(s) 95, 251
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 259
Bse1I ACTGG 2 cut(s) 190, 323
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 95, 251
BseLI CCNNNNNNNGG 2 cut(s) 173, 259
BseNI ACTGG 2 cut(s) 190, 323
BsiSI CCGG 1 cut(s) 253
BslFI GGGAC 1 cut(s) 116
BslI CCNNNNNNNGG 2 cut(s) 173, 259
BsmAI GTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 251
Bsp143I GATC 2 cut(s) 62, 118
BspACI CCGC 1 cut(s) 174
BspOI GCTAGC 1 cut(s) 345
BspPI GGATC 1 cut(s) 113
BsrI ACTGG 2 cut(s) 190, 323
BssECI CCNNGG 2 cut(s) 95, 251
BssMI GATC 2 cut(s) 62, 118
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 40, 307
Bst6I CTCTTC 1 cut(s) 198
BstAPI GCANNNNNTGC 1 cut(s) 41
BstBAI YACGTR 2 cut(s) 90, 116
BstC8I GCNNGC 2 cut(s) 172, 343
BstDEI CTNAG 2 cut(s) 158, 351
BstKTI GATC 2 cut(s) 65, 121
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 2 cut(s) 62, 118
BstMWI GCNNNNNNNGC 1 cut(s) 41
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 94, 251
BstSNI TACGTA 1 cut(s) 90
BsuI GTATCC 1 cut(s) 102
BtgZI GCGATG 1 cut(s) 266
Cac8I GCNNGC 2 cut(s) 172, 343
CviJI RGCY 2 cut(s) 249, 341
CviKI_1 RGCY 2 cut(s) 249, 341
DdeI CTNAG 2 cut(s) 158, 351
DpnI GATC 2 cut(s) 64, 120
DpnII GATC 2 cut(s) 62, 118
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
Eco105I TACGTA 1 cut(s) 90
Eco24I GRGCYC 1 cut(s) 251
Eco72I CACGTG 1 cut(s) 116
EcoRII CCWGG 1 cut(s) 94
EcoT38I GRGCYC 1 cut(s) 251
FaiI YATR 4 cut(s) 13, 15, 27, 59
FaqI GGGAC 1 cut(s) 116
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 346
FriOI GRGCYC 1 cut(s) 251
Fsp4HI GCNGC 1 cut(s) 346
FspBI CTAG 1 cut(s) 342
GluI GCNGC 1 cut(s) 346
HapII CCGG 1 cut(s) 253
HinfI GANTC 2 cut(s) 238, 273
HpaII CCGG 1 cut(s) 253
HphI GGTGA 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 242
HpyAV CCTTC 1 cut(s) 152
HpyCH4III ACNGT 2 cut(s) 40, 307
HpyCH4IV ACGT 2 cut(s) 89, 115
HpyCH4V TGCA 2 cut(s) 44, 266
HpyF10VI GCNNNNNNNGC 1 cut(s) 41
HpyF3I CTNAG 2 cut(s) 158, 351
HpySE526I ACGT 2 cut(s) 89, 115
Kzo9I GATC 2 cut(s) 62, 118
LpnPI CCDG 8 cut(s) 30, 81, 81, 108, 171, 180, 266, 336
MaeI CTAG 1 cut(s) 342
MaeII ACGT 2 cut(s) 89, 115
MaeIII GTNAC 3 cut(s) 77, 211, 301
MalI GATC 2 cut(s) 64, 120
MboI GATC 2 cut(s) 62, 118
MboII GAAGA 2 cut(s) 116, 185
MhlI GDGCHC 1 cut(s) 251
MluCI AATT 1 cut(s) 296
MnlI CCTC 2 cut(s) 144, 227
MspI CCGG 1 cut(s) 253
MspR9I CCNGG 2 cut(s) 96, 253
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 1 cut(s) 41
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 13
NdeII GATC 2 cut(s) 62, 118
NheI GCTAGC 1 cut(s) 341
NmuCI GTSAC 1 cut(s) 211
PfeI GAWTC 2 cut(s) 238, 273
PkrI GCNGC 1 cut(s) 347
PmaCI CACGTG 1 cut(s) 116
PmlI CACGTG 1 cut(s) 116
Ppu21I YACGTR 2 cut(s) 90, 116
PsiI TTATAA 1 cut(s) 59
Psp6I CCWGG 1 cut(s) 94
PspCI CACGTG 1 cut(s) 116
PspGI CCWGG 1 cut(s) 94
SatI GCNGC 1 cut(s) 346
Sau3AI GATC 2 cut(s) 62, 118
ScrFI CCNGG 2 cut(s) 96, 253
SduI GDGCHC 1 cut(s) 251
SetI ASST 5 cut(s) 70, 92, 118, 144, 155
SnaBI TACGTA 1 cut(s) 90
Sse9I AATT 1 cut(s) 296
SsiI CCGC 1 cut(s) 174
SspMI CTAG 1 cut(s) 342
StyD4I CCNGG 2 cut(s) 94, 251
TaaI ACNGT 2 cut(s) 40, 307
TaiI ACGT 2 cut(s) 92, 118
TasI AATT 1 cut(s) 296
TfiI GAWTC 2 cut(s) 238, 273
TseFI GTSAC 1 cut(s) 211
TseI GCWGC 1 cut(s) 345
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 4 cut(s) 17, 45, 117, 123
XapI RAATTY 1 cut(s) 296
XspI CTAG 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.