MD11G1141500.v1.1

SNF1-related protein kinase regulatory subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008047
Physical Location & Seq
Forward (+)
13242753 .. 13247660
4908 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1141500.v1.1.491

Sequence Viewer

Length: 354 bp
ATGAACAACTCATTTGGTGATGATTATGATGAAGCAACTGTTGCAGGATTCGAAGTTTTAAGATCACCCGATGCAAGTTACAACAACGTGTACCCTGGGAATGAAGACGAGGCACGGGATCCACCTCTTGTCCCTCCACATCTGCAACAGACATTACTTAGCTACCCAGCAAGTGCAGACACTGCCGGAACCCTTCCAGCACCGCAAAATGTGACACTCAACCATCTTTACATTGAGAACCGGGAGACCCCGCGCTCTGTGGTCGCACTTGGGTTCACTCATCGCTTTCGTTCAAAATTTGTTTCAGTTGTGCTTTACAAACCAGTTCAAAGAAGTGGGACTACCAGCAGTTAG

Protein Analysis

118

Amino Acids

12.88

Weight (kDa)

5.19

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMPKBI PF04739 38 - 108 8.7e-25 5'-AMP-activated protein kinase beta subunit, interaction domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 253
AciI CCGC 2 cut(s) 203, 251
AclWI GGATC 2 cut(s) 113, 126
AcsI RAATTY 1 cut(s) 296
AfaI GTAC 1 cut(s) 92
AflIII ACRYGT 1 cut(s) 87
AgsI TTSAA 2 cut(s) 294, 329
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 1 cut(s) 162
AluI AGCT 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 2 cut(s) 113, 126
AlwNI CAGNNNCTG 1 cut(s) 182
ApoI RAATTY 1 cut(s) 296
AspLEI GCGC 1 cut(s) 255
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 29, 57
AsuII TTCGAA 1 cut(s) 51
BamHI GGATCC 1 cut(s) 118
BarI GAAGNNNNNNTAC 1 cut(s) 325
BbsI GAAGAC 1 cut(s) 111
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 96
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 96, 242
BmiI GGNNCC 2 cut(s) 120, 190
BmrFI CCNGG 2 cut(s) 96, 242
BmsI GCATC 1 cut(s) 61
BpiI GAAGAC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 51
BpuMI CCSGG 1 cut(s) 242
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 94, 95
Bse1I ACTGG 1 cut(s) 323
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 94, 95
BseNI ACTGG 1 cut(s) 323
BseYI CCCAGC 1 cut(s) 166
BsgI GTGCAG 1 cut(s) 195
Bsh1236I CGCG 1 cut(s) 253
BsiSI CCGG 2 cut(s) 186, 241
BslFI GGGAC 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 239
BsmFI GGGAC 1 cut(s) 116
Bso31I GGTCTC 1 cut(s) 239
Bsp119I TTCGAA 1 cut(s) 51
Bsp143I GATC 2 cut(s) 62, 118
BspACI CCGC 2 cut(s) 203, 251
BspFNI CGCG 1 cut(s) 253
BspLI GGNNCC 2 cut(s) 120, 190
BspPI GGATC 2 cut(s) 113, 126
BspT104I TTCGAA 1 cut(s) 51
BspTNI GGTCTC 1 cut(s) 239
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 94, 95
BssMI GATC 2 cut(s) 62, 118
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 40
BstAPI GCANNNNNTGC 2 cut(s) 41, 182
BstBI TTCGAA 1 cut(s) 51
BstDEI CTNAG 1 cut(s) 158
BstFNI CGCG 1 cut(s) 253
BstHHI GCGC 1 cut(s) 255
BstKTI GATC 2 cut(s) 65, 121
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 2 cut(s) 62, 118
BstMWI GCNNNNNNNGC 2 cut(s) 41, 182
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 94, 240
BstUI CGCG 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
BtgZI GCGATG 1 cut(s) 266
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
CaiI CAGNNNCTG 1 cut(s) 182
CfoI GCGC 1 cut(s) 255
Csp6I GTAC 1 cut(s) 91
CviJI RGCY 1 cut(s) 162
CviKI_1 RGCY 1 cut(s) 162
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 158
DpnI GATC 2 cut(s) 64, 120
DpnII GATC 2 cut(s) 62, 118
Eco31I GGTCTC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 94
FaiI YATR 1 cut(s) 27
FaqI GGGAC 1 cut(s) 116
FauI CCCGC 1 cut(s) 258
GlaI GCGC 1 cut(s) 254
GsaI CCCAGC 1 cut(s) 170
HapII CCGG 2 cut(s) 186, 241
HhaI GCGC 1 cut(s) 255
Hin6I GCGC 1 cut(s) 253
HinP1I GCGC 1 cut(s) 253
HinfI GANTC 1 cut(s) 48
HpaII CCGG 2 cut(s) 186, 241
HphI GGTGA 2 cut(s) 29, 57
Hpy166II GTNNAC 2 cut(s) 91, 276
Hpy8I GTNNAC 2 cut(s) 91, 276
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 1 cut(s) 40
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 4 cut(s) 44, 74, 145, 176
HpyF10VI GCNNNNNNNGC 2 cut(s) 41, 182
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 1 cut(s) 87
HspAI GCGC 1 cut(s) 253
Kzo9I GATC 2 cut(s) 62, 118
LpnPI CCDG 8 cut(s) 30, 81, 108, 180, 199, 210, 254, 336
LweI GCATC 1 cut(s) 61
MaeII ACGT 1 cut(s) 87
MaeIII GTNAC 2 cut(s) 77, 211
MalI GATC 2 cut(s) 64, 120
MboI GATC 2 cut(s) 62, 118
MboII GAAGA 1 cut(s) 116
MflI RGATCY 1 cut(s) 118
MluCI AATT 1 cut(s) 296
MnlI CCTC 3 cut(s) 103, 135, 144
MseI TTAA 1 cut(s) 59
MspI CCGG 2 cut(s) 186, 241
MspR9I CCNGG 2 cut(s) 96, 242
MvaI CCWGG 1 cut(s) 96
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 41, 182
NciI CCSGG 1 cut(s) 242
NdeII GATC 2 cut(s) 62, 118
NlaIV GGNNCC 2 cut(s) 120, 190
NmuCI GTSAC 1 cut(s) 211
NspV TTCGAA 1 cut(s) 51
PasI CCCWGGG 1 cut(s) 95
PfeI GAWTC 1 cut(s) 48
Psp6I CCWGG 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 2 cut(s) 120, 190
PstNI CAGNNNCTG 1 cut(s) 182
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SaqAI TTAA 1 cut(s) 59
Sau3AI GATC 2 cut(s) 62, 118
ScrFI CCNGG 2 cut(s) 96, 242
SetI ASST 3 cut(s) 90, 127, 164
SfaNI GCATC 1 cut(s) 61
SfuI TTCGAA 1 cut(s) 51
Sse9I AATT 1 cut(s) 296
SsiI CCGC 2 cut(s) 203, 251
StyD4I CCNGG 2 cut(s) 94, 240
TaaI ACNGT 1 cut(s) 40
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 51
TasI AATT 1 cut(s) 296
TfiI GAWTC 1 cut(s) 48
Tru1I TTAA 1 cut(s) 59
Tru9I TTAA 1 cut(s) 59
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 1 cut(s) 211
Tsp45I GTSAC 1 cut(s) 211
TspDTI ATGAA 3 cut(s) 17, 45, 117
TspRI CASTG 1 cut(s) 187
XapI RAATTY 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.